FastQCFastQC Report
Thu 26 May 2016
SRR937042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891693
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131500.6951445081205038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89060.47079520831339966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59010.31194279410031117No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20190.10672979177911003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141700.032.7310141
GGTATCA92150.030.2706811
GTACATG113450.027.770811
ATCAACG170650.026.8441853
TCAACGC170700.026.782064
CAACGCA171900.026.5681615
TATCAAC173450.026.520432
TACATGG118900.026.1781482
AACGCAG174600.026.1566186
ACATGGG115800.025.6068123
GAGTACT91650.023.9011712-13
ACGCAGA193050.023.4592467
CGCAGAG193900.023.2583738
GCAGAGT198450.022.1504029
AGTACTT99250.022.0230812-13
CATGGGG39150.021.7281974
CATGGGA75700.021.7211444
GTACTTT100200.021.72029514-15
AGAGTAC166800.019.8278410-11
CATGGGT13500.019.7131824
Produced by FastQC (version 0.11.5)