##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1891693 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875993091902334 33.0 31.0 34.0 30.0 34.0 2 31.98806201640541 33.0 31.0 34.0 30.0 34.0 3 31.991451044117625 33.0 31.0 34.0 30.0 34.0 4 35.585235553549126 37.0 35.0 37.0 33.0 37.0 5 35.6008691685173 37.0 35.0 37.0 33.0 37.0 6 35.6834940976152 37.0 35.0 37.0 33.0 37.0 7 35.62889961531813 37.0 35.0 37.0 33.0 37.0 8 35.576898577094695 37.0 35.0 37.0 33.0 37.0 9 37.22412939097412 39.0 37.0 39.0 34.0 39.0 10-11 37.05034220668999 39.0 37.0 39.0 33.0 39.0 12-13 36.99094197631434 39.0 37.0 39.0 33.0 39.0 14-15 38.14303060803206 40.0 38.0 41.0 33.0 41.0 16-17 38.08758160018566 40.0 38.0 41.0 33.0 41.0 18-19 38.1076406161042 40.0 38.0 41.0 33.0 41.0 20-21 38.054533425878304 40.0 38.0 41.0 33.0 41.0 22-23 37.94595476115839 40.0 38.0 41.0 33.0 41.0 24-25 37.79448594460095 40.0 37.5 41.0 32.5 41.0 26-27 37.878902654923394 40.0 38.0 41.0 33.0 41.0 28-29 37.83214083892048 40.0 38.0 41.0 33.0 41.0 30-31 37.66375331515209 40.0 37.0 41.0 33.0 41.0 32-33 37.44723324556363 40.0 36.5 41.0 32.0 41.0 34-35 37.273446325593 40.0 36.0 41.0 32.0 41.0 36-37 37.0919136984701 40.0 36.0 41.0 31.0 41.0 38-39 36.926657761063765 39.0 36.0 41.0 31.0 41.0 40-41 36.75458094944581 39.0 36.0 41.0 30.5 41.0 42-43 36.510696503079515 39.0 35.0 41.0 30.0 41.0 44-45 36.204298741920596 39.0 35.0 41.0 30.0 41.0 46-47 35.779858042504785 39.0 35.0 40.5 28.5 41.0 48-49 35.39980853129974 39.0 35.0 40.0 26.5 41.0 50-51 34.726641426489394 38.0 34.0 39.5 25.0 40.5 52-53 34.95065319795548 38.5 35.0 40.0 26.5 40.5 54-55 35.335313393875225 39.0 35.0 40.5 26.5 41.0 56-57 35.240562554283386 39.0 35.0 41.0 26.0 41.0 58-59 35.01910352261176 39.0 35.0 41.0 26.0 41.0 60-61 34.75569397359931 38.0 34.5 40.0 25.5 41.0 62-63 34.45389579598803 38.0 34.0 40.0 24.5 41.0 64-65 34.15156449804488 37.5 34.0 40.0 23.5 41.0 66-67 33.789430420263756 37.0 34.0 40.0 22.5 41.0 68-69 33.417267495307115 36.5 33.5 39.0 22.0 41.0 70-71 33.00727998676318 36.0 33.0 39.0 21.5 41.0 72-73 32.619450936277715 35.5 33.0 39.0 20.0 40.0 74-75 32.192322961495336 35.0 32.0 37.5 18.5 40.0 76-77 31.76862762615287 35.0 32.0 37.0 18.0 39.0 78-79 31.326865934377302 35.0 31.5 36.5 14.0 39.0 80-81 30.899072418198934 35.0 31.0 36.0 9.5 38.5 82-83 30.493416214998945 35.0 31.0 36.0 7.0 37.0 84-85 30.137187958088337 34.0 31.0 35.0 4.5 37.0 86-87 29.819009744181535 34.0 30.0 35.0 2.0 36.5 88-89 29.46979742484642 34.0 30.0 35.0 2.0 36.0 90-91 29.13124804077617 34.0 29.5 35.0 2.0 36.0 92-93 28.790947315447063 34.0 29.0 35.0 2.0 35.5 94-95 28.403816845545236 34.0 29.0 35.0 2.0 35.0 96-97 27.971989905338763 34.0 29.0 35.0 2.0 35.0 98-99 27.412317167743392 34.0 28.0 35.0 2.0 35.0 100-101 25.212607172516893 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32008337931177167 1101 2 0.32622118885774043 1101 3 0.3161100163884214 1101 4 0.19548620987756493 1101 5 0.16761485005131505 1101 6 0.2099780411863108 1101 7 0.21749528734810752 1101 8 0.24592838691548735 1101 9 0.21254842884953007 1101 10-11 0.20851989176534147 1101 12-13 0.2307108302748162 1101 14-15 0.3514695439856794 1101 16-17 0.5617317502915498 1101 18-19 0.483607983770419 1101 20-21 0.3749268070432663 1101 22-23 0.5174073246995405 1101 24-25 0.4589422235011895 1101 26-27 0.5200866958865902 1101 28-29 0.541942189309033 1101 30-31 0.49273366443626543 1101 32-33 0.654978082624659 1101 34-35 0.6157997578322778 1101 36-37 0.6098113402577567 1101 38-39 0.583222889913479 1101 40-41 0.6777494597893821 1101 42-43 0.5611564208025541 1101 44-45 0.7328550453440812 1101 46-47 0.6297804775170377 1101 48-49 0.8294673616738208 1101 50-51 0.8250331838129057 1101 52-53 0.8122117314945569 1101 54-55 0.7600418670731415 1101 56-57 0.9367498699074588 1101 58-59 0.8394688441789455 1101 60-61 0.8472531455230339 1101 62-63 0.8814008464675069 1101 64-65 0.8809197026054179 1101 66-67 0.8747652857432726 1101 68-69 0.7819896278132177 1101 70-71 0.8397561540498941 1101 72-73 0.8381873043922141 1101 74-75 0.7446265194603541 1101 76-77 0.8301135135687367 1101 78-79 0.882407676247766 1101 80-81 0.7099834218926055 1101 82-83 0.7649648466119068 1101 84-85 0.7612895714739842 1101 86-87 0.7638280249931739 1101 88-89 0.9550359878072072 1101 90-91 0.9775499308195101 1101 92-93 0.9589650548264288 1101 94-95 0.8026484248041861 1101 96-97 0.8888184616735977 1101 98-99 1.0243135678177708 1101 100-101 1.2575209452544662 1102 1 0.15902682811485036 1102 2 0.20958781298617524 1102 3 0.1418327040652798 1102 4 0.16267165810113227 1102 5 0.12400236723614455 1102 6 -0.009801314821750395 1102 7 0.06223380048255933 1102 8 0.1270586710585846 1102 9 0.10217094822291983 1102 10-11 0.27779634099379535 1102 12-13 0.2751740778535279 1102 14-15 0.23751022040531922 1102 16-17 0.2713651199981584 1102 18-19 0.13578853084914755 1102 20-21 0.20495880319633386 1102 22-23 0.13734589085266435 1102 24-25 0.2715427894447089 1102 26-27 0.26317423218279856 1102 28-29 0.28909937118157103 1102 30-31 0.2921450646840995 1102 32-33 0.36546452895559156 1102 34-35 0.3004280414451941 1102 36-37 0.16585315719537164 1102 38-39 0.26390328595064005 1102 40-41 0.2484746751006881 1102 42-43 0.19915370205428928 1102 44-45 0.3233466702431258 1102 46-47 0.33728308221351 1102 48-49 0.5362525189111835 1102 50-51 0.4981322177633487 1102 52-53 0.5175997542062802 1102 54-55 0.5120678277283517 1102 56-57 0.5885612898831667 1102 58-59 0.5162491310962736 1102 60-61 0.54356865595107 1102 62-63 0.4668063776714604 1102 64-65 0.4928045164543846 1102 66-67 0.3859701042776109 1102 68-69 0.2749145632352423 1102 70-71 0.4635994530443952 1102 72-73 0.5350237541138654 1102 74-75 0.44215498617263904 1102 76-77 0.48740712605155423 1102 78-79 0.49677465794399467 1102 80-81 0.5528007787267732 1102 82-83 0.2922247675770926 1102 84-85 0.4590068401491969 1102 86-87 0.5295902917264215 1102 88-89 0.5506437605913632 1102 90-91 0.4956995791084253 1102 92-93 0.6114176308558221 1102 94-95 0.6532644586374978 1102 96-97 0.6498653030552894 1102 98-99 0.8762304086446164 1102 100-101 0.9629444164979972 1103 1 0.245293138004417 1103 2 0.3096463722572871 1103 3 0.3686007892996557 1103 4 0.299434292161763 1103 5 0.2393498350025567 1103 6 0.20812365207387984 1103 7 0.15294571337665985 1103 8 0.22765770603744784 1103 9 0.18824973476490925 1103 10-11 0.282079207152627 1103 12-13 0.16060715281310678 1103 14-15 0.3730877440425431 1103 16-17 0.4823422540324245 1103 18-19 0.3421780268680905 1103 20-21 0.4113744782172546 1103 22-23 0.553550154650857 1103 24-25 0.5785671541118944 1103 26-27 0.5375520817021808 1103 28-29 0.4824510743763071 1103 30-31 0.3577829708392457 1103 32-33 0.4879303644296513 1103 34-35 0.4247664182282449 1103 36-37 0.346911312455795 1103 38-39 0.4223834444951322 1103 40-41 0.41217615204199376 1103 42-43 0.5561405063519516 1103 44-45 0.5546926459920911 1103 46-47 0.4963427064687025 1103 48-49 0.5851259306255585 1103 50-51 0.4881816905071048 1103 52-53 0.5921505893721459 1103 54-55 0.4206222529451509 1103 56-57 0.5766612624787655 1103 58-59 0.5463445860501963 1103 60-61 0.660605690512547 1103 62-63 0.6253253405522372 1103 64-65 0.5552163711976874 1103 66-67 0.7067995293273199 1103 68-69 0.57955988788828 1103 70-71 0.4607188344921269 1103 72-73 0.386884579855284 1103 74-75 0.42211412464953213 1103 76-77 0.5592637520126509 1103 78-79 0.5361318167684708 1103 80-81 0.44636633280441984 1103 82-83 0.696256816616863 1103 84-85 0.5472069613524084 1103 86-87 0.6764515222212069 1103 88-89 0.6759852944506548 1103 90-91 0.6711393677751829 1103 92-93 0.6319777092517214 1103 94-95 0.7049196213966233 1103 96-97 0.7344300706488802 1103 98-99 0.7956626733548049 1103 100-101 0.8185253526888161 1104 1 0.2829178149477478 1104 2 0.23997663194359475 1104 3 0.2561646587496895 1104 4 0.28145944606892215 1104 5 0.22721941452979877 1104 6 0.2362002036901032 1104 7 0.2814425758826644 1104 8 0.16453834098206244 1104 9 0.17395420063555633 1104 10-11 0.3090178439209197 1104 12-13 0.32545740019762803 1104 14-15 0.43835139605318574 1104 16-17 0.4087972365239594 1104 18-19 0.38602190214528065 1104 20-21 0.4260037791199238 1104 22-23 0.33416610704080085 1104 24-25 0.4997147808930933 1104 26-27 0.26269390290548955 1104 28-29 0.2912605532231751 1104 30-31 0.41315577418878746 1104 32-33 0.4861365703550149 1104 34-35 0.3653260414052326 1104 36-37 0.2405642403338959 1104 38-39 0.35592360603695994 1104 40-41 0.4076220107117834 1104 42-43 0.38136563436062687 1104 44-45 0.4532280864552405 1104 46-47 0.634075725423159 1104 48-49 0.42435178163371745 1104 50-51 0.43301952819912515 1104 52-53 0.49288027929168976 1104 54-55 0.6332289897895294 1104 56-57 0.5422665587197102 1104 58-59 0.6289918432383743 1104 60-61 0.593866364196991 1104 62-63 0.5884079081715683 1104 64-65 0.568801639274028 1104 66-67 0.4742567833321445 1104 68-69 0.5396598128905765 1104 70-71 0.6138216353057757 1104 72-73 0.6128460882264335 1104 74-75 0.6242397431108344 1104 76-77 0.6296690094058697 1104 78-79 0.8298879069599536 1104 80-81 1.0010362080060808 1104 82-83 0.8419834041132859 1104 84-85 0.752499317521373 1104 86-87 0.6976663771626939 1104 88-89 0.8684848762979733 1104 90-91 0.8785847516393446 1104 92-93 0.7406305022418636 1104 94-95 0.7431738474364735 1104 96-97 0.7744497103361532 1104 98-99 0.9184181646437395 1104 100-101 0.76852864395509 1105 1 0.1309152620073526 1105 2 0.2542974233551156 1105 3 0.3397710483259857 1105 4 0.22933297918188345 1105 5 0.20641824884757654 1105 6 0.21162839320979998 1105 7 0.2192112818911056 1105 8 0.16797975280626787 1105 9 0.2434883492239166 1105 10-11 0.3240906689541916 1105 12-13 0.1812104875093965 1105 14-15 0.33584662434265766 1105 16-17 0.2976131899930081 1105 18-19 0.3329878167783207 1105 20-21 0.3249858543473749 1105 22-23 0.450916406589819 1105 24-25 0.44594190829324276 1105 26-27 0.37079455137644146 1105 28-29 0.3561246272102849 1105 30-31 -0.02771583819779977 1105 32-33 0.2318668377514328 1105 34-35 0.24703061080727906 1105 36-37 0.2515239412063579 1105 38-39 0.29297736831244237 1105 40-41 0.29443956578966635 1105 42-43 0.4471170959792161 1105 44-45 0.6040381031336466 1105 46-47 0.5296604631747712 1105 48-49 0.5730475542293405 1105 50-51 0.45606463215317206 1105 52-53 0.43114018548318 1105 54-55 0.5366746163738298 1105 56-57 0.662167210803311 1105 58-59 0.5783772710064241 1105 60-61 0.6945497574401216 1105 62-63 0.7407611291514442 1105 64-65 0.6394177769882958 1105 66-67 0.6100881447598638 1105 68-69 0.6475388854025113 1105 70-71 0.5308947854776775 1105 72-73 0.6459887939819922 1105 74-75 0.6926379536441232 1105 76-77 0.6980807071714388 1105 78-79 0.4651900149421131 1105 80-81 0.7022481321644811 1105 82-83 0.6696175388531458 1105 84-85 0.5696632656677814 1105 86-87 0.7340266307708276 1105 88-89 0.7893409542830767 1105 90-91 0.633933944512787 1105 92-93 0.6071719303230019 1105 94-95 0.6881260701203118 1105 96-97 0.49972923924639545 1105 98-99 0.3531955952055661 1105 100-101 0.4124264540861695 1106 1 0.21380029008155432 1106 2 0.2860168577555662 1106 3 0.19940740661742495 1106 4 0.14822647361114605 1106 5 0.049246834554729446 1106 6 0.07191148695704896 1106 7 0.196009647414229 1106 8 0.2340129126969117 1106 9 0.130427232723342 1106 10-11 0.35406097791735647 1106 12-13 0.33419649450589617 1106 14-15 0.296472928837467 1106 16-17 0.40993570520598865 1106 18-19 0.45009470451092426 1106 20-21 0.4545240264377952 1106 22-23 0.27509990307687104 1106 24-25 0.2745643617723843 1106 26-27 0.24117553735403163 1106 28-29 0.29108361830297014 1106 30-31 0.3425732819480132 1106 32-33 0.40679775464592893 1106 34-35 0.4782752825890384 1106 36-37 0.3280959493199447 1106 38-39 0.289905374897387 1106 40-41 0.34765927946224195 1106 42-43 0.3747635668614251 1106 44-45 0.266512013237417 1106 46-47 0.3130592049803411 1106 48-49 0.35005243414232723 1106 50-51 0.23362438169016286 1106 52-53 0.32949615455746795 1106 54-55 0.48965033439716876 1106 56-57 0.41358929387941856 1106 58-59 0.514611359844352 1106 60-61 0.6518137724914226 1106 62-63 0.5678255381234223 1106 64-65 0.5646536884443378 1106 66-67 0.649454490998508 1106 68-69 0.46027957388180596 1106 70-71 0.3794117034892537 1106 72-73 0.47107625025385147 1106 74-75 0.36711689064621567 1106 76-77 0.6235040249243262 1106 78-79 0.3905251414964823 1106 80-81 0.5665916956768413 1106 82-83 0.722800506193007 1106 84-85 0.747537563812827 1106 86-87 0.6720061704127716 1106 88-89 0.7250422212966292 1106 90-91 0.7256821880380819 1106 92-93 0.6735137594084613 1106 94-95 0.5740287070371473 1106 96-97 0.6486841735439555 1106 98-99 0.6570730682337569 1106 100-101 0.6622794687553046 1107 1 0.251413304443421 1107 2 0.21886262818988556 1107 3 0.28876137728210693 1107 4 0.26588882186356244 1107 5 0.2208635387654354 1107 6 0.22502747256459799 1107 7 0.1580036947299135 1107 8 0.2896119015560146 1107 9 0.3560247945690804 1107 10-11 0.359935686144091 1107 12-13 0.30462189738235423 1107 14-15 0.3516134896537224 1107 16-17 0.3513733702044135 1107 18-19 0.2912928351234214 1107 20-21 0.39246015739685447 1107 22-23 0.31016416964296667 1107 24-25 0.4651842971490723 1107 26-27 0.30869620356541816 1107 28-29 0.32536965293861897 1107 30-31 0.364102071942618 1107 32-33 0.4691498294214327 1107 34-35 0.5698976948531964 1107 36-37 0.6251940157168434 1107 38-39 0.3055724526798045 1107 40-41 0.5962839953336854 1107 42-43 0.6090129068677399 1107 44-45 0.6631766243253168 1107 46-47 0.6154252441008836 1107 48-49 0.560654378957679 1107 50-51 0.438456350866673 1107 52-53 0.6249218122577318 1107 54-55 0.7125617984109525 1107 56-57 0.8450358267465887 1107 58-59 0.8239376566427481 1107 60-61 0.9231664792299838 1107 62-63 0.7158898964260985 1107 64-65 0.804332304649094 1107 66-67 0.6906623049159677 1107 68-69 0.6293352987536309 1107 70-71 0.5816057365348115 1107 72-73 0.6173420620715646 1107 74-75 0.5324022423540384 1107 76-77 0.6707073335567451 1107 78-79 0.5752726620607618 1107 80-81 0.49364200809084124 1107 82-83 0.5402663852842018 1107 84-85 0.6108337608222136 1107 86-87 0.375330601900842 1107 88-89 0.4054080463651779 1107 90-91 0.43999360312568925 1107 92-93 0.592713204911842 1107 94-95 0.37741735412654975 1107 96-97 0.3509617888007668 1107 98-99 0.5186125098638144 1107 100-101 0.617179730714188 1108 1 0.22655185728995875 1108 2 0.23005645741833192 1108 3 0.195783628455672 1108 4 0.24184369913055548 1108 5 0.22098658196497212 1108 6 0.2307014184255891 1108 7 0.24578364385826745 1108 8 0.20640729829278825 1108 9 0.2438161174385698 1108 10-11 0.27103232158466284 1108 12-13 0.3433670635423667 1108 14-15 0.45378225529261584 1108 16-17 0.4409454590436681 1108 18-19 0.4659394736659337 1108 20-21 0.44667076806335615 1108 22-23 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-1.6532468544072216 2316 68-69 -1.762955867221173 2316 70-71 -1.7374460441075286 2316 72-73 -2.021227597822108 2316 74-75 -2.319572607741847 2316 76-77 -2.7108065158837 2316 78-79 -2.721566606747043 2316 80-81 -2.5397058908240275 2316 82-83 -2.5902108802706287 2316 84-85 -2.639740365849793 2316 86-87 -3.0454836141067325 2316 88-89 -3.033789147332783 2316 90-91 -2.4215799651990224 2316 92-93 -2.430934016703482 2316 94-95 -3.0532698985620144 2316 96-97 -3.2212519368322283 2316 98-99 -3.286501223051971 2316 100-101 -3.2227809422156817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3165.0 3 1064.0 4 1123.0 5 1216.0 6 1319.0 7 1570.0 8 1922.0 9 2523.0 10 3248.0 11 4309.0 12 6283.0 13 8011.0 14 9129.0 15 12110.0 16 16193.0 17 22395.0 18 19420.0 19 10887.0 20 9886.0 21 10063.0 22 10886.0 23 11768.0 24 13515.0 25 15365.0 26 17940.0 27 21199.0 28 25869.0 29 31098.0 30 38531.0 31 48045.0 32 61242.0 33 81876.0 34 113575.0 35 163834.0 36 251667.0 37 384825.0 38 382529.0 39 72070.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28426009118009 19.91715419969402 15.088078528469056 21.710507180656833 2 14.6838006848319 21.09610121762583 41.43032033739208 22.789777760150187 3 18.14605975171661 27.12917440855826 30.76353645730336 23.961229382421774 4 11.813839452869663 17.59938737648634 39.65219442334908 30.934578747294918 5 12.578740771811637 38.3948704575785 34.63625981796726 14.390128952642605 6 28.789157069417037 38.18701241273084 18.728746365492956 14.29508415235917 7 26.824573504488704 34.13836587104951 22.146293831763046 16.890766792698745 8 25.73339024946297 34.26155288994822 20.863675819266955 19.14138104132185 9 26.821123744800417 16.767096738702676 21.637726020557132 34.774053495939775 10-11 23.66216956427328 27.453748189243825 28.40458528294611 20.479496963536786 12-13 25.33498127289313 25.44424146422939 28.672323369964108 20.548453892913372 14-15 22.580626391974086 26.03252376125051 27.831494470717267 23.555355376058138 16-17 20.581589871853794 29.635416143280036 28.167438942927365 21.615555041938805 18-19 20.500463416536764 27.673939860300738 31.453091801427668 20.37250492173483 20-21 21.672814568371816 26.659239292694686 31.298580169228828 20.36936596970467 22-23 21.328833104285565 26.78473510744447 31.19493137291709 20.69150041535288 24-25 20.98805901495465 26.997200464210092 31.491658229287005 20.523082291548256 26-27 20.894240900807258 27.007934086860992 31.462326172547222 20.63549883978453 28-29 20.515432195042514 26.88440802938348 31.797136834402824 20.803022941171186 30-31 20.736610541541896 26.843072755417957 31.689891767751106 20.730424935289044 32-33 20.678514842115668 27.02674483212911 31.430530524550594 20.864209801204623 34-35 21.028869057138333 26.941219604197357 31.21967556839764 20.810235770266665 36-37 21.226666455204352 26.589482921774803 31.1501699628355 21.033680660185347 38-39 20.79669562086764 27.07019981755708 31.237178177373536 20.895926384201744 40-41 20.828902498369743 26.996175406348204 31.39719392347165 20.7777281718104 42-43 20.816532164804627 27.093303348286206 30.84983977929906 21.240324707610107 44-45 20.92335295090954 27.14354134519163 30.63738356194074 21.29572214195809 46-47 21.26198269274535 27.443911601316216 30.022501704214 21.271604001724434 48-49 21.24714704277024 27.625956327122353 29.686402676989076 21.44049395311833 50-51 21.19533483770711 27.616078596084627 29.61759001858484 21.570996547623423 52-53 21.331735749480664 27.948386728423696 29.274825393124836 21.445052128970797 54-55 21.29985255501696 27.742310154671852 29.332828633403345 21.625008656907845 56-57 21.318588529888707 28.13095314257193 29.01998220302823 21.530476124511125 58-59 21.49247403122465 27.611343181162756 29.298028257770543 21.598154529842052 60-61 21.458704425400118 27.72591080466771 29.54699142476619 21.268393345165983 62-63 21.410335374960678 27.679470674847273 29.69896350183588 21.21123044835617 64-65 21.342612956297767 27.72605005054115 29.497924429350242 21.433412563810844 66-67 21.295080268414363 27.986643784194175 28.955606522985043 21.76266942440642 68-69 21.14739128549659 27.880837842725242 29.468792399475902 21.50297847230227 70-71 21.316862201872695 27.740339115676832 29.427167321387977 21.5156313610625 72-73 21.339973527692692 27.782519976493735 29.1283938626755 21.749112633138072 74-75 21.14082081256452 28.3165134037325 28.88909156518532 21.65357421851765 76-77 21.233623660448142 27.944053096947403 29.17981818974941 21.642505052855046 78-79 21.213618712125477 27.896377134054685 29.028484733588378 21.861519420231456 80-81 21.126240349459234 28.337644201241517 28.76217413685479 21.77394131244446 82-83 21.302971487583967 28.207359276112847 28.912865817594675 21.576803418708508 84-85 21.169766578060003 28.03973949376559 28.743481726362706 22.0470122018117 86-87 21.141345383097477 28.00055306810034 28.88453672351036 21.973564825291813 88-89 21.387717680984554 27.680992487555706 28.841367324608076 22.089922506851664 90-91 21.08882921436355 27.73940173556642 29.19593776154969 21.975831288520336 92-93 21.268377726121912 27.80015101938082 29.10657970714085 21.82489154735642 94-95 20.981140734791467 27.60257040334987 29.167394781944783 22.248894079913878 96-97 21.108664768408143 27.617837143379155 28.94105677245461 22.332441315758093 98-99 20.88966742457539 27.725840138909387 28.934712483914932 22.449779952600295 100-101 20.89796501287322 27.65083223026073 29.113076732859838 22.33812602400621 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 612.0 1 446.5 2 360.0 3 462.0 4 425.5 5 445.5 6 764.5 7 1583.5 8 2783.0 9 3407.5 10 3023.5 11 2669.0 12 2757.0 13 2922.5 14 3124.5 15 3201.0 16 3314.0 17 3537.0 18 3752.5 19 3980.5 20 4426.0 21 5030.5 22 5854.5 23 7017.0 24 8205.0 25 10212.0 26 13563.0 27 16457.0 28 18229.5 29 21090.0 30 25774.0 31 30687.5 32 36696.0 33 43796.0 34 51313.5 35 57151.5 36 64050.0 37 70734.5 38 70758.0 39 68953.5 40 67206.0 41 65959.5 42 64748.5 43 65735.0 44 69071.5 45 70077.5 46 67426.5 47 65405.5 48 70491.5 49 68037.5 50 57560.5 51 52235.0 52 49137.0 53 47586.0 54 45891.5 55 42813.5 56 39163.0 57 35341.5 58 32575.5 59 29621.5 60 25546.0 61 21002.0 62 16970.0 63 13421.0 64 10207.5 65 7604.0 66 5340.5 67 3996.5 68 2758.5 69 1629.0 70 1135.0 71 767.5 72 536.0 73 425.5 74 339.0 75 248.0 76 167.0 77 85.5 78 39.0 79 24.0 80 18.0 81 13.5 82 12.0 83 11.0 84 7.5 85 7.0 86 7.0 87 5.5 88 4.0 89 2.0 90 1.5 91 2.5 92 4.0 93 3.5 94 1.5 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037532517168483465 2 0.037955418770381874 3 0.02468688101081941 4 0.042765924491976234 5 0.06100355607384496 6 0.05994630206909894 7 0.06179649657740448 8 0.06301233868286238 9 0.061162144174556864 10-11 0.06626339474745638 12-13 0.06615766934698178 14-15 0.061717202527048515 16-17 0.05085391762828323 18-19 0.044695413050637706 20-21 0.049664506872943964 22-23 0.028017231125769353 24-25 0.016281711673088606 26-27 0.012369871855528354 28-29 0.015330183068817192 30-31 0.010836853548646636 32-33 0.007929405035595099 34-35 0.008960227690222462 36-37 0.0054448581244419675 38-39 0.007532934783815345 40-41 0.006317092678357429 42-43 0.004414035469814605 44-45 0.0011629794052206146 46-47 0.0030924679638820885 48-49 8.193718536781602E-4 50-51 0.0051541132731368145 52-53 0.004572623570526508 54-55 0.007400778033222093 56-57 0.019030572085428236 58-59 0.007532934783815345 60-61 0.01670461327498701 62-63 0.01205269565410455 64-65 0.00872234553915461 66-67 0.015277320368579893 68-69 0.0240525286079718 70-71 0.016017398171902102 72-73 0.014933712817037436 74-75 0.017735435929614372 76-77 0.023021705953344438 78-79 0.035021538907211686 80-81 0.028678014878735607 82-83 0.030105307785142726 84-85 0.0198499439391064 86-87 0.02246664760085278 88-89 0.01926845423649609 90-91 0.017021789476410816 92-93 0.02881017162932886 94-95 0.020854335243615112 96-97 0.034968676206974386 98-99 0.05095964302875784 100-101 0.06028990962064141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1891693.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.85544482643072 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12963570913027 35.20232500798993 2 13.451131013138976 12.605174540784486 3 4.590575238861279 6.4528033447813105 4 2.108814625773233 3.952377893883515 5 1.1472450562060474 2.687733871672893 6 0.7035922287218079 1.9780276111908055 7 0.48438853081893385 1.5887368056239672 8 0.3419754797185369 1.2818730577555268 9 0.23839720432341413 1.0053186348555956 >10 1.5100636936984757 13.665009698622221 >50 0.1730564963664409 5.641026582872056 >100 0.113547931873875 9.79297726112934 >500 0.006219754108656345 1.967254056244208 >1k 0.0010177779450528564 0.6979997759825721 >5k 2.2617287667841252E-4 0.7845807851646508 >10k+ 1.1308643833920626E-4 0.6967810714469614 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13150 0.6951445081205038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8906 0.47079520831339966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5901 0.31194279410031117 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2019 0.10672979177911003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.9294050355951E-5 0.0 18-19 0.0 0.0 0.0 1.3215675059325164E-4 0.0 20-21 0.0 0.0 0.0 2.3788215106785297E-4 2.643135011865033E-5 22-23 0.0 0.0 0.0 5.021956522543562E-4 5.286270023730066E-5 24-25 0.0 0.0 0.0 8.458032037968106E-4 5.286270023730066E-5 26-27 0.0 0.0 0.0 0.0015065869567630688 5.286270023730066E-5 28-29 2.643135011865033E-5 0.0 0.0 0.002986742563407487 5.286270023730066E-5 30-31 5.286270023730066E-5 0.0 0.0 0.006872151030849085 5.286270023730066E-5 32-33 5.286270023730066E-5 0.0 0.0 0.011735519452680747 5.286270023730066E-5 34-35 5.286270023730066E-5 0.0 0.0 0.015805947370952898 5.286270023730066E-5 36-37 5.286270023730066E-5 0.0 0.0 0.018713395884004433 5.286270023730066E-5 38-39 5.286270023730066E-5 0.0 0.0 0.021197942795157566 5.286270023730066E-5 40-41 5.286270023730066E-5 0.0 0.0 0.024739743711056708 5.286270023730066E-5 42-43 5.286270023730066E-5 0.0 0.0 0.02947095538229512 7.9294050355951E-5 44-45 5.286270023730066E-5 0.0 0.0 0.033937853552347026 1.0572540047460132E-4 46-47 5.286270023730066E-5 0.0 0.0 0.041259337535213166 1.0572540047460132E-4 48-49 5.286270023730066E-5 0.0 0.0 0.04781431236463845 1.0572540047460132E-4 50-51 5.286270023730066E-5 0.0 0.0 0.05706528490616607 1.0572540047460132E-4 52-53 5.286270023730066E-5 0.0 0.0 0.0800869908595105 1.0572540047460132E-4 54-55 5.286270023730066E-5 0.0 0.0 0.1082363787358731 1.0572540047460132E-4 56-57 5.286270023730066E-5 0.0 0.0 0.14328434899320344 1.0572540047460132E-4 58-59 5.286270023730066E-5 0.0 0.0 0.19144226890938434 1.0572540047460132E-4 60-61 5.286270023730066E-5 0.0 0.0 0.2616703661746383 1.0572540047460132E-4 62-63 5.286270023730066E-5 0.0 0.0 0.3058107208727843 1.0572540047460132E-4 64-65 5.286270023730066E-5 0.0 0.0 0.3464357060051499 1.0572540047460132E-4 66-67 5.286270023730066E-5 0.0 0.0 0.43960621517339227 1.0572540047460132E-4 68-69 5.286270023730066E-5 0.0 0.0 0.564045011531998 1.0572540047460132E-4 70-71 5.286270023730066E-5 0.0 0.0 0.6544666602879008 1.0572540047460132E-4 72-73 5.286270023730066E-5 0.0 0.0 0.7359545127036998 1.58588100711902E-4 74-75 5.286270023730066E-5 0.0 0.0 0.9169299669660986 1.58588100711902E-4 76-77 5.286270023730066E-5 0.0 0.0 1.038064844559873 1.58588100711902E-4 78-79 5.286270023730066E-5 0.0 0.0 1.1246803788986903 1.58588100711902E-4 80-81 5.286270023730066E-5 0.0 0.0 1.2409783194207518 1.58588100711902E-4 82-83 5.286270023730066E-5 0.0 0.0 1.4670985196858053 2.1145080094920265E-4 84-85 5.286270023730066E-5 0.0 0.0 1.708839647870981 2.1145080094920265E-4 86-87 5.286270023730066E-5 0.0 0.0 2.039865876756958 2.1145080094920265E-4 88-89 5.286270023730066E-5 0.0 0.0 2.462185988952753 2.643135011865033E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14170 0.0 32.731014 1 GGTATCA 9215 0.0 30.270681 1 GTACATG 11345 0.0 27.77081 1 ATCAACG 17065 0.0 26.844185 3 TCAACGC 17070 0.0 26.78206 4 CAACGCA 17190 0.0 26.568161 5 TATCAAC 17345 0.0 26.52043 2 TACATGG 11890 0.0 26.178148 2 AACGCAG 17460 0.0 26.156618 6 ACATGGG 11580 0.0 25.606812 3 GAGTACT 9165 0.0 23.90117 12-13 ACGCAGA 19305 0.0 23.459246 7 CGCAGAG 19390 0.0 23.258373 8 GCAGAGT 19845 0.0 22.150402 9 AGTACTT 9925 0.0 22.02308 12-13 CATGGGG 3915 0.0 21.728197 4 CATGGGA 7570 0.0 21.721144 4 GTACTTT 10020 0.0 21.720295 14-15 AGAGTAC 16680 0.0 19.82784 10-11 CATGGGT 1350 0.0 19.713182 4 >>END_MODULE