FastQCFastQC Report
Thu 26 May 2016
SRR937042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891693
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131430.6947744692188426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96810.5117638009973077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.3660741991433071No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29250.15462339819410442No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.12454452175908036No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19050.10070344395205777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161950.029.9218961
GGTATCA111500.029.1867391
ATCAACG198600.024.248193
TCAACGC198750.024.2053554
TATCAAC201950.023.9883232
CAACGCA201000.023.9343995
AACGCAG205000.023.4667666
GAGTACT109700.023.07466712-13
GTACATG111550.022.9130331
AGTACTT116600.021.99429712-13
GTACTTT116100.021.76321414-15
TAGGACG1100.00288129621.586714
CGATTCG1100.00288173821.586149
TACATGG117250.021.5497572
ACATGGG112850.021.3787753
ACGCAGA224650.021.3084457
CGCAGAG225300.021.2048138
ACCGCTC1604.9999726E-520.7766598
CATTCCG4600.020.647619
GTATAGG9100.020.3607181
Produced by FastQC (version 0.11.5)