FastQCFastQC Report
Thu 26 May 2016
SRR937041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952926
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129930.665309387042827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87060.44579262091856015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59940.30692407187983567No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21580.1105008587114924No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.10184717700517071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141550.032.7324521
GGTATCA93200.030.2868671
ATCAACG171250.026.9175853
TCAACGC171500.026.8783454
GTACATG110150.026.7911221
CAACGCA173100.026.6573565
TATCAAC174450.026.4510652
AACGCAG177250.026.0573626
GTATAGG10400.025.1311971
TACATGG115150.024.8856092
ACATGGG112850.024.508483
ACGCAGA194900.023.6232767
CGCAGAG195100.023.4772878
GAGTACT89100.023.46015212-13
CATTCCG4350.022.9357919
AGTACTT95800.022.73681812-13
GCAGAGT199150.022.4965049
CATGGGA72050.022.4253834
GTACTTT98000.021.25306314-15
AGAGTAC165150.020.49698410-11
Produced by FastQC (version 0.11.5)