##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937041_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1952926 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.852887923044705 33.0 31.0 34.0 30.0 34.0 2 31.970690645728514 33.0 31.0 34.0 30.0 34.0 3 31.971262095952433 33.0 31.0 34.0 30.0 34.0 4 35.5530644786336 37.0 35.0 37.0 33.0 37.0 5 35.55126563935346 37.0 35.0 37.0 33.0 37.0 6 35.661241644588685 37.0 35.0 37.0 33.0 37.0 7 35.60853969889284 37.0 35.0 37.0 33.0 37.0 8 35.57389271278072 37.0 35.0 37.0 33.0 37.0 9 37.17887057676533 39.0 37.0 39.0 34.0 39.0 10-11 37.06197546655633 39.0 37.0 39.0 33.0 39.0 12-13 36.969564899028434 39.0 37.0 39.0 33.0 39.0 14-15 38.1283184821135 40.0 38.0 41.0 33.0 41.0 16-17 38.063148834108404 40.0 38.0 41.0 33.0 41.0 18-19 38.05821956387493 40.0 38.0 41.0 33.0 41.0 20-21 37.968303970554956 40.0 38.0 41.0 33.0 41.0 22-23 37.83609081962143 40.0 37.5 41.0 33.0 41.0 24-25 37.95297261647395 40.0 38.0 41.0 33.0 41.0 26-27 37.90269626191673 40.0 38.0 41.0 33.0 41.0 28-29 37.8054485935463 40.0 37.5 41.0 33.0 41.0 30-31 37.60950645339352 40.0 37.0 41.0 32.5 41.0 32-33 37.38768212415627 40.0 36.5 41.0 32.0 41.0 34-35 37.19662342556758 40.0 36.0 41.0 31.0 41.0 36-37 37.00135028157749 39.0 36.0 41.0 31.0 41.0 38-39 36.83438952628005 39.0 36.0 41.0 31.0 41.0 40-41 36.64259014422461 39.0 35.0 41.0 30.0 41.0 42-43 36.39395143492381 39.0 35.0 41.0 30.0 41.0 44-45 36.078765145223116 39.0 35.0 40.5 29.5 41.0 46-47 35.66269945712229 39.0 35.0 40.0 27.5 41.0 48-49 35.338366891525844 39.0 35.0 40.0 26.5 41.0 50-51 34.61875539574976 38.0 34.0 39.5 25.0 40.5 52-53 34.808439234256696 38.5 34.5 40.0 26.0 40.5 54-55 35.214345295213434 39.0 35.0 40.5 26.0 41.0 56-57 35.135084995540026 39.0 35.0 41.0 26.0 41.0 58-59 34.89751736624941 38.5 35.0 40.5 26.0 41.0 60-61 34.63487684633212 38.0 34.0 40.0 25.0 41.0 62-63 34.31379811626247 38.0 34.0 40.0 23.5 41.0 64-65 33.988765319320855 37.5 34.0 40.0 23.0 41.0 66-67 33.62293937404694 37.0 33.5 40.0 22.0 41.0 68-69 33.23600535811393 36.0 33.0 39.0 21.5 41.0 70-71 32.84551770010743 36.0 33.0 39.0 20.5 40.5 72-73 32.450492747805086 35.5 32.0 38.5 19.5 40.0 74-75 32.01046813857771 35.0 32.0 37.5 18.0 40.0 76-77 31.572868864462862 35.0 31.5 37.0 15.5 39.0 78-79 31.13901473993382 35.0 31.0 36.5 12.0 39.0 80-81 30.69626268481243 35.0 31.0 36.0 7.5 38.5 82-83 30.278576607613395 34.5 30.5 36.0 6.5 37.0 84-85 29.884064987613456 34.0 30.0 35.0 2.0 37.0 86-87 29.541646739302976 34.0 30.0 35.0 2.0 36.0 88-89 29.181649995954785 34.0 29.5 35.0 2.0 36.0 90-91 28.8030772799379 34.0 29.0 35.0 2.0 36.0 92-93 28.458068303663325 34.0 29.0 35.0 2.0 35.0 94-95 28.063712859575837 34.0 29.0 35.0 2.0 35.0 96-97 27.574061946023555 34.0 27.5 35.0 2.0 35.0 98-99 26.950586709378648 34.0 26.5 35.0 2.0 35.0 100-101 24.400142401709026 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2706829416512342 1101 2 0.34865970696007764 1101 3 0.23090811860630112 1101 4 0.28933769576333646 1101 5 0.3374789033841026 1101 6 0.11010014590999617 1101 7 0.19996073598868946 1101 8 0.11099742404817903 1101 9 0.25772403813213884 1101 10-11 0.28272150291501674 1101 12-13 0.4255578267335878 1101 14-15 0.4542750273201648 1101 16-17 0.5281040170819438 1101 18-19 0.544156800475804 1101 20-21 0.5293055250996019 1101 22-23 0.5318487238851475 1101 24-25 0.5154543384424315 1101 26-27 0.5438019613990264 1101 28-29 0.5416764774285454 1101 30-31 0.6074210346371203 1101 32-33 0.6097286175929924 1101 34-35 0.6027559118859145 1101 36-37 0.4444386811440495 1101 38-39 0.7660188663474585 1101 40-41 0.6606897683196635 1101 42-43 0.5273723389019764 1101 44-45 0.5767556597097894 1101 46-47 0.5103570063870393 1101 48-49 0.6103202844262015 1101 50-51 0.5290174113896597 1101 52-53 0.679806074402606 1101 54-55 0.6176807987501007 1101 56-57 0.553226236868241 1101 58-59 0.6167814875022373 1101 60-61 0.7007411611641814 1101 62-63 0.7667282257660304 1101 64-65 0.7860163607315229 1101 66-67 0.7708756328432784 1101 68-69 0.7228903946010234 1101 70-71 0.6092539541188131 1101 72-73 0.5500054748311314 1101 74-75 0.4537801949726621 1101 76-77 0.38584419002258485 1101 78-79 0.4554440265608619 1101 80-81 0.39769991891616385 1101 82-83 0.34468698396547026 1101 84-85 0.2995164715323497 1101 86-87 0.2790493551930133 1101 88-89 0.4032931880636603 1101 90-91 0.3773388068076642 1101 92-93 0.5120006937578978 1101 94-95 0.538071158910622 1101 96-97 0.5890401713698488 1101 98-99 0.49111830645367505 1101 100-101 0.606563762974698 1102 1 0.07361663725577827 1102 2 0.16255111324431937 1102 3 0.006473743743249827 1102 4 -0.02893164119270608 1102 5 0.12946400351995635 1102 6 0.080857146654985 1102 7 0.17872737782224846 1102 8 0.09300417281017559 1102 9 0.2636727650702184 1102 10-11 0.13674788443918828 1102 12-13 0.13957728420324145 1102 14-15 0.07279214025237479 1102 16-17 0.11815984774936794 1102 18-19 0.2496190030263108 1102 20-21 0.2906749102610817 1102 22-23 0.2242784534964386 1102 24-25 -0.06248562386967649 1102 26-27 0.17846036010185884 1102 28-29 0.059454381806475 1102 30-31 0.016299425451791194 1102 32-33 0.24315479753076374 1102 34-35 0.15382519625421054 1102 36-37 0.0504158054702728 1102 38-39 0.19556862751139903 1102 40-41 0.1138359184577098 1102 42-43 0.2150619834963976 1102 44-45 0.0888107015608739 1102 46-47 0.05046893066067071 1102 48-49 -0.026626295178523662 1102 50-51 0.11498840047769932 1102 52-53 0.1383889220295842 1102 54-55 0.22402165505405947 1102 56-57 0.2523895656731838 1102 58-59 0.10274704252218214 1102 60-61 0.07977503644355721 1102 62-63 0.21356691282506546 1102 64-65 0.296444425063882 1102 66-67 0.13690911371448777 1102 68-69 0.20713124652266401 1102 70-71 0.15788272838586437 1102 72-73 0.015471884402110447 1102 74-75 0.013498324601478373 1102 76-77 0.07374208213004252 1102 78-79 0.050729972483122054 1102 80-81 0.12387880493220393 1102 82-83 0.19343486949945188 1102 84-85 0.15932321152971696 1102 86-87 0.13560187721413186 1102 88-89 0.23881560359457765 1102 90-91 0.2013914822097398 1102 92-93 0.11489564971565613 1102 94-95 0.2324675829432259 1102 96-97 0.32576211361096696 1102 98-99 0.16026033721751176 1102 100-101 0.23186215466582993 1103 1 0.17022547708712565 1103 2 0.32718382914748645 1103 3 0.3061207189670867 1103 4 0.2688166178437186 1103 5 0.37442643660536845 1103 6 0.2802107872822077 1103 7 0.20482354533373837 1103 8 0.24455886512151181 1103 9 0.3028132264847798 1103 10-11 0.3813943385582874 1103 12-13 0.44414832025420736 1103 14-15 0.5583948689462943 1103 16-17 0.5689308932749739 1103 18-19 0.569652543222432 1103 20-21 0.529837447567445 1103 22-23 0.4718295454477399 1103 24-25 0.3603165931941348 1103 26-27 0.48166183814440444 1103 28-29 0.464186297103268 1103 30-31 0.5255463817263646 1103 32-33 0.5589100677946348 1103 34-35 0.3782271101298065 1103 36-37 0.6479210402042739 1103 38-39 0.7268820009540136 1103 40-41 0.7152115402304133 1103 42-43 0.6334716665699602 1103 44-45 0.6958847458074047 1103 46-47 0.7211755486908942 1103 48-49 0.7401638891030657 1103 50-51 0.6672745343542488 1103 52-53 0.7866445475921395 1103 54-55 0.8007277046063876 1103 56-57 0.8091905510348809 1103 58-59 0.8384082296935276 1103 60-61 0.906803128726466 1103 62-63 0.9523474329823429 1103 64-65 0.8916768851519947 1103 66-67 0.7249095874460707 1103 68-69 0.8515193274360229 1103 70-71 0.9640194473209149 1103 72-73 0.7420478472615528 1103 74-75 0.7679282833868335 1103 76-77 0.765929679837182 1103 78-79 0.8127921260669027 1103 80-81 0.8030400157901312 1103 82-83 0.8186630660033138 1103 84-85 0.695754117778236 1103 86-87 0.7955127209300876 1103 88-89 0.7280478741900573 1103 90-91 0.8364600311698993 1103 92-93 0.8368597408616125 1103 94-95 0.9091077838054211 1103 96-97 0.8541605218728456 1103 98-99 0.9046716309784664 1103 100-101 1.0863377811758532 1104 1 0.27546822153660955 1104 2 0.25901289413597794 1104 3 0.2725972376461314 1104 4 0.2336795251385979 1104 5 0.2888722800842345 1104 6 0.10125560479942664 1104 7 0.19192294619966077 1104 8 0.21430282247440857 1104 9 0.37527731305925727 1104 10-11 0.42661702066067164 1104 12-13 0.38654908958033474 1104 14-15 0.49303427945574896 1104 16-17 0.4283015723972383 1104 18-19 0.3220830142750657 1104 20-21 0.5317583789512383 1104 22-23 0.5397604288170541 1104 24-25 0.4939194283740136 1104 26-27 0.4172680887740725 1104 28-29 0.44533757897489323 1104 30-31 0.30260173581083194 1104 32-33 0.3469369013393404 1104 34-35 0.39541650755872126 1104 36-37 0.40518726659659876 1104 38-39 0.4151008343527778 1104 40-41 0.451242110122962 1104 42-43 0.5631082391631992 1104 44-45 0.4703820366235405 1104 46-47 0.4546206919154798 1104 48-49 0.4817003066784977 1104 50-51 0.2766986964848712 1104 52-53 0.4321392674925093 1104 54-55 0.42650336370154207 1104 56-57 0.4601044678526307 1104 58-59 0.4932823767121022 1104 60-61 0.47039774244854726 1104 62-63 0.6075145964052737 1104 64-65 0.4843649079846841 1104 66-67 0.46135993676474385 1104 68-69 0.3807564980439224 1104 70-71 0.3923281694874703 1104 72-73 0.46009143881554593 1104 74-75 0.47678305877685645 1104 76-77 0.3267995623113791 1104 78-79 0.5042396333233867 1104 80-81 0.5489340547564403 1104 82-83 0.5372131913981661 1104 84-85 0.6113879888576506 1104 86-87 0.5993340404180891 1104 88-89 0.5337329106808077 1104 90-91 0.3421083965591798 1104 92-93 0.318460342193049 1104 94-95 0.48703200874749086 1104 96-97 0.5384759248548754 1104 98-99 0.5255033249263086 1104 100-101 0.5844000586150173 1105 1 0.2222679434128203 1105 2 0.2639789727906958 1105 3 0.274322810040033 1105 4 0.3269521532127797 1105 5 0.22534666772072143 1105 6 0.2597558135271427 1105 7 0.12481085885278986 1105 8 0.2285237330577914 1105 9 0.3614288977151503 1105 10-11 0.2679422539647547 1105 12-13 0.4180304449234882 1105 14-15 0.3936724669842846 1105 16-17 0.4319967386589312 1105 18-19 0.4474226042695335 1105 20-21 0.36297431176630823 1105 22-23 0.3692992338514287 1105 24-25 0.20675965624128878 1105 26-27 0.16747990750624098 1105 28-29 0.11268764064904246 1105 30-31 -0.08781564993800117 1105 32-33 0.13435081099047608 1105 34-35 -0.12403563444626542 1105 36-37 0.056870610124654775 1105 38-39 0.1352284358903333 1105 40-41 0.0667036074459233 1105 42-43 0.13948313887385666 1105 44-45 0.036476674692828226 1105 46-47 0.22278826486733294 1105 48-49 0.024477349731704123 1105 50-51 7.548077110115514E-4 1105 52-53 0.12082328025310574 1105 54-55 0.16520199976649508 1105 56-57 0.20840682698165125 1105 58-59 0.285217259059138 1105 60-61 0.3484920999662009 1105 62-63 0.3934726948496703 1105 64-65 0.43190737232955456 1105 66-67 0.3438076778407577 1105 68-69 0.4889989611973462 1105 70-71 0.4387940843746705 1105 72-73 0.22091813546167316 1105 74-75 0.24057207045953888 1105 76-77 0.36641095783320665 1105 78-79 0.30468132230716805 1105 80-81 0.2744188886700982 1105 82-83 0.212065986090348 1105 84-85 0.2523385463528669 1105 86-87 0.3144997221192263 1105 88-89 0.287556606908133 1105 90-91 0.3201864784852049 1105 92-93 0.4135255333559442 1105 94-95 0.45807011049197044 1105 96-97 0.1998215521047122 1105 98-99 -0.030573394256119713 1105 100-101 0.42329530562522066 1106 1 0.09906487088993643 1106 2 0.21502359946746452 1106 3 0.2613611595513561 1106 4 0.1962840231578511 1106 5 0.31951229572239015 1106 6 0.18872371748265948 1106 7 0.1800011844937046 1106 8 0.2195056769498862 1106 9 0.2239185388369549 1106 10-11 0.3275964322122604 1106 12-13 0.2521636058495389 1106 14-15 0.40129934148762914 1106 16-17 0.21893007376965556 1106 18-19 0.11525050210785537 1106 20-21 0.3653500959491254 1106 22-23 0.31535456562805564 1106 24-25 0.39618178821135785 1106 26-27 0.2550239998537478 1106 28-29 0.4533753146393167 1106 30-31 0.2585034027698967 1106 32-33 0.2809392130460324 1106 34-35 0.3944822257779421 1106 36-37 0.2786461385107586 1106 38-39 0.2469624991404018 1106 40-41 0.19871774365803674 1106 42-43 0.2392671854238202 1106 44-45 0.4498716391856661 1106 46-47 0.5458386295665036 1106 48-49 0.3691026934539181 1106 50-51 0.302448294522371 1106 52-53 0.43853257402755474 1106 54-55 0.31058066258806605 1106 56-57 0.48451916128589545 1106 58-59 0.3511804477347624 1106 60-61 0.21580921901682615 1106 62-63 0.35533610058962495 1106 64-65 0.3814345483234689 1106 66-67 0.3077917375730692 1106 68-69 0.3891938080859063 1106 70-71 0.34271035131268235 1106 72-73 0.29279116135016636 1106 74-75 0.4585102778533532 1106 76-77 0.41250139315418366 1106 78-79 0.40405212460089857 1106 80-81 0.4254507773518732 1106 82-83 0.5342820861754412 1106 84-85 0.4892526633839367 1106 86-87 0.509939816143401 1106 88-89 0.40759147346652824 1106 90-91 0.39456785257419824 1106 92-93 0.4141550272946546 1106 94-95 0.4726969575938149 1106 96-97 0.4510685055391903 1106 98-99 0.3685183570774804 1106 100-101 0.22275297808249306 1107 1 0.13809113430922082 1107 2 0.2606679594137944 1107 3 0.19071719019722622 1107 4 0.1311510655742083 1107 5 0.2823044830972776 1107 6 0.22503292393602692 1107 7 0.06851420976697398 1107 8 0.20909175663459934 1107 9 0.12983391117872856 1107 10-11 0.2512110016917788 1107 12-13 0.2799166461215492 1107 14-15 0.1731776310062827 1107 16-17 0.3082190836770238 1107 18-19 0.0820965482406919 1107 20-21 0.34120234370956837 1107 22-23 0.3367482767030978 1107 24-25 0.22846296381575115 1107 26-27 0.0890618638612537 1107 28-29 0.1548509475451496 1107 30-31 0.02829617408011842 1107 32-33 0.10799060326130672 1107 34-35 0.20656600096869937 1107 36-37 0.26409889833757205 1107 38-39 0.26971229749717907 1107 40-41 0.45811746692346844 1107 42-43 0.3729546882312036 1107 44-45 0.31269944368645497 1107 46-47 0.36829030842940824 1107 48-49 0.15738731994036215 1107 50-51 0.031855297449865816 1107 52-53 0.18357425021491025 1107 54-55 0.31893823704959345 1107 56-57 0.3225537599426431 1107 58-59 0.18137293156912193 1107 60-61 0.23050842396641968 1107 62-63 0.2146372237374834 1107 64-65 0.16661115946745753 1107 66-67 0.1940977948768463 1107 68-69 0.17792163465516353 1107 70-71 0.16879209640050163 1107 72-73 0.17141793195342103 1107 74-75 0.2300758494947992 1107 76-77 0.08328221624912047 1107 78-79 0.1526208483213658 1107 80-81 0.10955824000637548 1107 82-83 0.13586486340313186 1107 84-85 0.22706960453926683 1107 86-87 -0.026460813295923202 1107 88-89 -0.05052325029690863 1107 90-91 0.12024750740787127 1107 92-93 0.08561755583229669 1107 94-95 -0.15808575987327345 1107 96-97 -0.14639486667759272 1107 98-99 -0.2572270637172558 1107 100-101 -0.2521768617942364 1108 1 -0.12261630093000164 1108 2 0.10933454298673695 1108 3 0.06858035576369303 1108 4 0.2161243106632753 1108 5 0.2306538040439392 1108 6 0.13467634719452803 1108 7 0.09744533585187298 1108 8 0.14862275598571273 1108 9 0.0471446335042387 1108 10-11 0.012512144769665667 1108 12-13 0.13099540565274737 1108 14-15 0.28001197890766605 1108 16-17 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-2.0178948433577872 2316 76-77 -2.3249695394435754 2316 78-79 -2.3951210724520493 2316 80-81 -2.6885161983239705 2316 82-83 -2.269426535982756 2316 84-85 -1.9463259507460506 2316 86-87 -2.171428967614471 2316 88-89 -2.3517328541639912 2316 90-91 -2.315698210526037 2316 92-93 -2.8333868676012592 2316 94-95 -2.238060982565031 2316 96-97 -2.430511168018306 2316 98-99 -2.465814377127405 2316 100-101 -2.4065030102148732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3192.0 3 1089.0 4 1082.0 5 1280.0 6 1421.0 7 1656.0 8 2040.0 9 2604.0 10 3396.0 11 4907.0 12 6656.0 13 8345.0 14 9884.0 15 12591.0 16 16900.0 17 23463.0 18 20491.0 19 11058.0 20 10444.0 21 10918.0 22 11559.0 23 12784.0 24 14622.0 25 16723.0 26 19728.0 27 23023.0 28 28263.0 29 33779.0 30 41954.0 31 52371.0 32 66416.0 33 88304.0 34 122200.0 35 175515.0 36 264297.0 37 393458.0 38 370100.0 39 64397.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19474925543602 19.92337969305782 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27.667581575532985 31.39858146875521 20.38208519842358 20-21 21.73240838800999 26.667467270824076 31.16354243270316 20.436581908462777 22-23 21.405220139667335 26.814474521746657 31.076468813995206 20.703836524590802 24-25 21.037764350453173 27.047314250601666 31.35526652670388 20.559654872241282 26-27 21.005481006448782 27.042294485300516 31.29301878309777 20.659205725152923 28-29 20.56778136108582 26.914990255427618 31.706652014817077 20.810576368669484 30-31 20.824393755830993 26.83565583130134 31.582635491564965 20.7573149213027 32-33 20.719096451831433 27.093797348897013 31.309631767355906 20.877474431915648 34-35 21.057070262774932 26.990782036800166 31.10332905599086 20.848818644434044 36-37 21.29194859405835 26.60730616521056 31.02380735368365 21.076937887047436 38-39 20.86035049989119 27.052498175813206 31.171268737918762 20.915882586376846 40-41 20.93274935475009 26.985412397963977 31.299657872253704 20.782180375032226 42-43 20.897269480394794 27.077255911005288 30.764647899944954 21.260826708654967 44-45 20.963380816821132 27.13015787348666 30.548236759792815 21.358224549899393 46-47 21.32835550348554 27.42328690385606 29.942404371696625 21.305953220961776 48-49 21.292952061235532 27.61190495148671 29.61191160819051 21.483231379087247 50-51 21.23503013942171 27.57664916811919 29.537836597839956 21.650484094619145 52-53 21.411413979367317 27.924990309387837 29.242606331746142 21.42098937949871 54-55 21.32674617445269 27.676846192861298 29.288610907780033 21.70779672490598 56-57 21.341807490125035 28.105429925885268 28.94635105006969 21.60641153392001 58-59 21.57840121890116 27.628050805299694 29.170008907236472 21.62353906856268 60-61 21.496702028351137 27.71315199652613 29.449752594518724 21.340393380604002 62-63 21.420853896958445 27.710634846345044 29.618968273556725 21.24954298313979 64-65 21.37741131469697 27.6735525711089 29.448167535245616 21.500868578948516 66-67 21.2979826882724 27.982240180056305 28.891739609698767 21.82803752197253 68-69 21.196723582417306 27.835978371280724 29.425868990923508 21.541429055378465 70-71 21.38043852923537 27.712484782182262 29.344203605566456 21.562873083015916 72-73 21.386988392855084 27.780149218990662 29.039371699809514 21.79349068834474 74-75 21.24599604066069 28.263926250071382 28.844153060652257 21.645924648615676 76-77 21.25280455602543 27.965838004285487 29.100772458152218 21.68058498153686 78-79 21.269057448518193 27.89074779407585 28.957463011815634 21.882731745590327 80-81 21.1890703451625 28.35925832106687 28.65677819478485 21.794893138985785 82-83 21.35482826726547 28.215942337794313 28.82731251178076 21.601916883159454 84-85 21.213524151386054 28.053341923542867 28.650183946055808 22.08294997901527 86-87 21.227359728579202 27.92896968921038 28.865243414524855 21.97842716768556 88-89 21.43813871122283 27.699150642580843 28.752276001856902 22.11043464433942 90-91 21.17192291490345 27.690558471306915 29.148368708603122 21.98914990518651 92-93 21.290001055119223 27.811406146120692 28.990092123176463 21.90850067558362 94-95 21.060681052876582 27.619382437837558 29.06436205991981 22.25557444936605 96-97 21.209426286493905 27.60409371385435 28.813299781548075 22.373180218103673 98-99 20.99145104781836 27.675885030255397 28.911402350315228 22.421261571611016 100-101 20.992383910142586 27.624966801101397 28.975127910202005 22.40752137855401 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 607.0 1 444.0 2 351.5 3 429.5 4 382.0 5 416.0 6 728.0 7 1570.5 8 2791.0 9 3406.0 10 3067.5 11 2658.0 12 2676.0 13 2843.5 14 3060.0 15 3265.5 16 3381.0 17 3535.5 18 3792.0 19 4035.0 20 4475.5 21 5169.0 22 5959.5 23 6941.0 24 8244.0 25 10411.5 26 13625.0 27 16643.5 28 18621.0 29 21709.0 30 26427.5 31 31555.5 32 37737.5 33 45034.5 34 52736.5 35 58490.5 36 65867.0 37 73012.5 38 72606.5 39 70474.0 40 69073.0 41 68074.5 42 67268.5 43 68307.5 44 71247.5 45 72430.0 46 69904.5 47 67927.5 48 73125.0 49 70370.5 50 60027.5 51 54058.5 52 50726.5 53 49263.0 54 47354.5 55 44586.0 56 40921.0 57 36809.5 58 33863.5 59 30967.0 60 26811.0 61 22098.5 62 17831.5 63 13925.0 64 10556.5 65 8040.0 66 5665.0 67 4201.0 68 2908.0 69 1693.0 70 1169.5 71 837.5 72 581.5 73 416.5 74 318.0 75 235.0 76 154.0 77 90.0 78 58.0 79 33.5 80 17.0 81 13.5 82 15.0 83 14.5 84 15.0 85 9.5 86 5.5 87 5.5 88 4.5 89 4.5 90 2.5 91 2.0 92 2.0 93 1.5 94 3.5 95 3.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022581500783951877 2 0.009319349529884901 3 0.010804300828602825 4 0.017921826019009424 5 0.05110280676277545 6 0.037738245074314135 7 0.015207949507559427 8 0.040912968540538656 9 0.02396404164827546 10-11 0.020840523399248103 12-13 0.03282254422338583 14-15 0.01725615819544622 16-17 0.014695897335587727 18-19 0.013953421686228767 20-21 0.01198202082413773 22-23 0.007322346059195279 24-25 0.0013313356471264144 26-27 0.0 28-29 0.0022530295566754706 30-31 0.0 32-33 1.5361565159150936E-4 34-35 0.0 36-37 0.0 38-39 5.1205217197169784E-5 40-41 2.5602608598584892E-5 42-43 5.1205217197169784E-5 44-45 7.680782579575468E-5 46-47 0.0 48-49 3.840391289787734E-4 50-51 1.0241043439433957E-4 52-53 2.5602608598584894E-4 54-55 0.0018177852104995272 56-57 4.0964173757735827E-4 58-59 9.985017353448108E-4 60-61 0.003021107814633017 62-63 0.0022530295566754706 64-65 0.00120332260413349 66-67 0.010420261699624052 68-69 0.012519675604708014 70-71 0.022786321652740554 72-73 0.014977526030172163 74-75 0.016769708632073103 76-77 0.026754725985521213 78-79 0.017563389498629237 80-81 0.0403241085427712 82-83 0.030211078146330176 84-85 0.01615524602570707 86-87 0.012212444301524994 88-89 0.010983519088792918 90-91 0.02091733122504386 92-93 0.027650817286471688 94-95 0.010215440830835372 96-97 0.016513682546087257 98-99 0.023784823388085366 100-101 0.03469153465108253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1952926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.68269045570807 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85116270312801 34.942536587199655 2 13.507983525524981 12.611780272057734 3 4.720427212334086 6.610867271162793 4 2.175779277575747 4.062849220600509 5 1.1779714129188368 2.7495437417481567 6 0.7003619484413539 1.9616868027626582 7 0.4706379656115955 1.5379452525745205 8 0.3485666376666587 1.3017622759503664 9 0.2504488657865528 1.0522464188847138 >10 1.5010106368738088 13.523698834621303 >50 0.17332634804863276 5.620737439842836 >100 0.11440403591068724 9.836411606037322 >500 0.00659952514926581 2.077407645332511 >1k 9.899287723898716E-4 0.6885668229746644 >5k 2.199841716421937E-4 0.7548047947596992 >10k+ 1.0999208582109685E-4 0.6671550134906648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12993 0.665309387042827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8706 0.44579262091856015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5994 0.30692407187983567 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2158 0.1105008587114924 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1989 0.10184717700517071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.1205217197169784E-5 0.0 12-13 0.0 0.0 0.0 1.0241043439433957E-4 0.0 14-15 0.0 0.0 0.0 2.0482086878867916E-4 0.0 16-17 0.0 0.0 0.0 2.5602608598584894E-4 0.0 18-19 0.0 0.0 0.0 2.5602608598584894E-4 0.0 20-21 0.0 0.0 0.0 3.328339117816036E-4 0.0 22-23 0.0 0.0 0.0 5.888599977674526E-4 0.0 24-25 0.0 0.0 0.0 9.472965181476409E-4 0.0 26-27 0.0 0.0 0.0 0.0015105539073165088 0.0 28-29 0.0 0.0 0.0 0.0027394791200485834 0.0 30-31 0.0 0.0 0.0 0.006221433889456129 0.0 32-33 0.0 0.0 0.0 0.011239545174778768 0.0 34-35 0.0 0.0 0.0 0.015566386027939614 0.0 36-37 0.0 0.0 0.0 0.01815224949639669 0.0 38-39 0.0 0.0 0.0 0.020533292096065085 0.0 40-41 0.0 0.0 0.0 0.024296875560057062 0.0 42-43 0.0 0.0 0.0 0.0284701007616264 0.0 44-45 0.0 0.0 0.0 0.03295055726637876 0.0 46-47 0.0 0.0 0.0 0.03976085115360234 0.0 48-49 0.0 0.0 0.0 0.04744163373317781 0.0 50-51 0.0 0.0 0.0 0.057477856303823086 0.0 52-53 0.0 0.0 0.0 0.08011056230497213 0.0 54-55 0.0 0.0 0.0 0.10927193349876033 0.0 56-57 0.0 0.0 0.0 0.1439122629326457 0.0 58-59 0.0 0.0 0.0 0.19171233318620368 0.0 60-61 0.0 0.0 0.0 0.2587655651058975 0.0 62-63 0.0 0.0 0.0 0.3036725405878154 0.0 64-65 0.0 0.0 0.0 0.3471713725968112 0.0 66-67 0.0 0.0 0.0 0.4425410896265399 0.0 68-69 0.0 0.0 0.0 0.5636926335150436 0.0 70-71 0.0 0.0 0.0 0.6531737505670978 0.0 72-73 0.0 0.0 0.0 0.7369454859016675 0.0 74-75 0.0 0.0 0.0 0.9095070678561297 0.0 76-77 0.0 0.0 0.0 1.0309402404392178 0.0 78-79 0.0 0.0 0.0 1.1234680679145037 0.0 80-81 0.0 0.0 0.0 1.2449524457147891 0.0 82-83 0.0 0.0 0.0 1.4692568996469912 0.0 84-85 0.0 0.0 0.0 1.7050057196227608 0.0 86-87 0.0 0.0 0.0 2.0229133105913895 0.0 88-89 0.0 0.0 0.0 2.4273577186232353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14155 0.0 32.732452 1 GGTATCA 9320 0.0 30.286867 1 ATCAACG 17125 0.0 26.917585 3 TCAACGC 17150 0.0 26.878345 4 GTACATG 11015 0.0 26.791122 1 CAACGCA 17310 0.0 26.657356 5 TATCAAC 17445 0.0 26.451065 2 AACGCAG 17725 0.0 26.057362 6 GTATAGG 1040 0.0 25.131197 1 TACATGG 11515 0.0 24.885609 2 ACATGGG 11285 0.0 24.50848 3 ACGCAGA 19490 0.0 23.623276 7 CGCAGAG 19510 0.0 23.477287 8 GAGTACT 8910 0.0 23.460152 12-13 CATTCCG 435 0.0 22.935791 9 AGTACTT 9580 0.0 22.736818 12-13 GCAGAGT 19915 0.0 22.496504 9 CATGGGA 7205 0.0 22.425383 4 GTACTTT 9800 0.0 21.253063 14-15 AGAGTAC 16515 0.0 20.496984 10-11 >>END_MODULE