FastQCFastQC Report
Thu 26 May 2016
SRR937041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952926
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132390.6779058704733308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98820.5060099563424317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67310.34466231695414984No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28600.14644692118390557No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.12022984997895467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168300.029.7554861
TATCGCG856.4063835E-427.9429767
GGTATCA114200.027.6256521
GTACATG117950.024.8540861
ATCAACG205150.024.169783
TCAACGC206300.024.0580714
TATCAAC207050.024.0180322
CAACGCA207150.023.9865825
AACGCAG210150.023.6667656
TACATGG125300.023.123032
ACATGGG120750.022.6951163
AGTACTT115900.022.08762712-13
GAGTACT109350.022.02079212-13
TACCGTG1100.002877003321.59237
CATTCCG3550.021.409839
ACGCAGA231800.021.3948217
CATATAG6900.021.3381273
CGCAGAG232550.021.2032578
CATGGGA78000.020.7636434
GTACTTT117300.020.32588414-15
Produced by FastQC (version 0.11.5)