##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937041_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1952926 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24204962195188 33.0 31.0 34.0 31.0 34.0 2 32.36154518911623 34.0 31.0 34.0 31.0 34.0 3 32.460617555401484 34.0 31.0 34.0 31.0 34.0 4 36.100469244610395 37.0 35.0 37.0 35.0 37.0 5 36.021159531902384 37.0 35.0 37.0 35.0 37.0 6 36.07390551408502 37.0 35.0 37.0 35.0 37.0 7 36.02466504107171 37.0 35.0 37.0 35.0 37.0 8 35.99412778569183 37.0 35.0 37.0 35.0 37.0 9 37.67795502748184 39.0 37.0 39.0 35.0 39.0 10-11 37.60153994570199 39.0 37.0 39.0 35.0 39.0 12-13 37.509953270118785 39.0 37.0 39.0 35.0 39.0 14-15 38.70426273192123 40.0 38.0 41.0 34.5 41.0 16-17 38.633868359579424 40.0 38.0 41.0 34.0 41.0 18-19 38.822540895046714 40.0 38.0 41.0 35.0 41.0 20-21 38.86222519440061 40.0 38.0 41.0 35.0 41.0 22-23 38.77565330176361 40.0 38.0 41.0 35.0 41.0 24-25 38.672114816434416 40.0 38.0 41.0 34.0 41.0 26-27 38.49360267618947 40.0 38.0 41.0 34.0 41.0 28-29 38.41033761647907 40.0 38.0 41.0 34.0 41.0 30-31 38.362304050435085 40.0 38.0 41.0 34.0 41.0 32-33 38.17049980388402 40.0 37.0 41.0 34.0 41.0 34-35 38.01126463573121 40.0 37.0 41.0 33.0 41.0 36-37 37.840773536734105 40.0 37.0 41.0 33.0 41.0 38-39 37.66974836732165 40.0 37.0 41.0 33.0 41.0 40-41 37.48030442525728 40.0 37.0 41.0 33.0 41.0 42-43 37.200724195386826 40.0 36.5 41.0 32.5 41.0 44-45 36.86842332991624 40.0 36.0 41.0 31.0 41.0 46-47 36.433833130389985 39.5 36.0 41.0 30.5 41.0 48-49 36.009705180841465 39.0 35.5 41.0 29.5 41.0 50-51 35.70932718392811 39.0 35.0 40.5 28.0 41.0 52-53 35.468206936668366 39.0 35.0 40.0 27.5 41.0 54-55 35.181615432433176 38.5 35.0 40.0 27.0 41.0 56-57 34.91802735997165 38.0 34.5 40.0 26.0 41.0 58-59 34.58726111486047 38.0 34.0 40.0 26.0 41.0 60-61 34.26904091604085 38.0 34.0 40.0 25.0 41.0 62-63 33.918784173081825 37.0 33.5 40.0 24.0 41.0 64-65 33.53629579410587 37.0 33.0 39.0 23.0 40.5 66-67 33.1499892981096 36.0 33.0 39.0 22.0 40.0 68-69 32.85166079001458 36.0 32.0 39.0 22.0 40.0 70-71 32.39387232286323 35.0 32.0 38.5 20.5 40.0 72-73 31.900026165865988 35.0 31.0 37.5 18.5 39.5 74-75 31.39241271814703 35.0 31.0 37.0 18.0 39.0 76-77 29.969484506837432 33.5 29.5 35.5 15.0 38.0 78-79 30.704476001650857 34.0 31.0 36.0 15.5 38.0 80-81 30.46798009755618 34.0 31.0 36.0 9.5 37.5 82-83 30.14570239732586 34.0 30.0 35.0 7.5 37.0 84-85 29.791016147053192 34.0 30.0 35.0 7.0 36.5 86-87 29.449039031688862 34.0 30.0 35.0 2.0 36.0 88-89 29.067216320536467 34.0 29.0 35.0 2.0 36.0 90-91 28.616642156436036 34.0 29.0 35.0 2.0 35.0 92-93 28.16316798485964 33.5 29.0 35.0 2.0 35.0 94-95 27.593312035376663 33.0 27.0 35.0 2.0 35.0 96-97 26.95069296020433 33.0 26.0 34.5 2.0 35.0 98-99 26.16618243599604 32.5 25.0 34.0 2.0 35.0 100-101 22.60618118658874 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.161155702682926 1101 2 0.1525995765833983 1101 3 0.12138540642419571 1101 4 0.12528176604481445 1101 5 0.1184170346197817 1101 6 0.09529601602487503 1101 7 0.13697241454413955 1101 8 0.14202217798213468 1101 9 0.18786784290595904 1101 10-11 0.18851555411787047 1101 12-13 0.21566876707726834 1101 14-15 0.21955068463324778 1101 16-17 0.2538897718426725 1101 18-19 0.15494069489663786 1101 20-21 0.21528971635863314 1101 22-23 0.18703090235329256 1101 24-25 0.24534199906914722 1101 26-27 0.11616236745438613 1101 28-29 0.26339295957961184 1101 30-31 0.21158596374770156 1101 32-33 0.32194686848074383 1101 34-35 0.30025081364561856 1101 36-37 0.23534186299883686 1101 38-39 0.21498531132117904 1101 40-41 0.3956636177957975 1101 42-43 0.4455197338350345 1101 44-45 0.43313843560162724 1101 46-47 0.576406718490027 1101 48-49 0.6238250426275016 1101 50-51 0.5557797845866119 1101 52-53 0.44818493081778144 1101 54-55 0.428532963075007 1101 56-57 0.4619002705077193 1101 58-59 0.4489143422674218 1101 60-61 0.4843072786114817 1101 62-63 0.43003556592501724 1101 64-65 0.40883484674741766 1101 66-67 0.25180784536771483 1101 68-69 0.2765566870195215 1101 70-71 0.20640743132974393 1101 72-73 0.41254739347716907 1101 74-75 0.43967124062034557 1101 76-77 0.34400611191410846 1101 78-79 0.32946963636497983 1101 80-81 0.5099206976223023 1101 82-83 0.46993585215777145 1101 84-85 0.3574742832046205 1101 86-87 -0.04692471233852302 1101 88-89 0.21765094905656568 1101 90-91 0.4037076311462897 1101 92-93 0.4621973861043571 1101 94-95 0.34732894094806355 1101 96-97 0.47977590702872774 1101 98-99 0.47442362694746265 1101 100-101 0.7528437852729155 1102 1 0.09947096627904983 1102 2 0.17301589631695435 1102 3 0.17247265827572278 1102 4 0.11296343482959514 1102 5 0.09163111060908591 1102 6 0.011280239698130856 1102 7 -0.011559072374659252 1102 8 0.03992462769509331 1102 9 0.0426986418016142 1102 10-11 0.10811238132328072 1102 12-13 0.06007430632087818 1102 14-15 0.10740124776047111 1102 16-17 0.1527869189569131 1102 18-19 0.12037406182770383 1102 20-21 0.04282168533185171 1102 22-23 0.09919629084533454 1102 24-25 0.12127775436078991 1102 26-27 0.06898370055399994 1102 28-29 0.019415046437053718 1102 30-31 -0.0020009594303544986 1102 32-33 -0.1065381659204121 1102 34-35 0.009792160768633096 1102 36-37 0.001274725963909873 1102 38-39 0.043327657611548887 1102 40-41 0.06643998836418064 1102 42-43 0.07695020843952705 1102 44-45 0.03237810429877186 1102 46-47 -0.037173455926030385 1102 48-49 0.12815906252319564 1102 50-51 0.2170346160866714 1102 52-53 0.2380081483119696 1102 54-55 0.20369602894117378 1102 56-57 0.12211018329968937 1102 58-59 0.25204392726237757 1102 60-61 0.22775493188288465 1102 62-63 0.2317852482073235 1102 64-65 0.0376553910306896 1102 66-67 0.05203441094902672 1102 68-69 0.04691086555495616 1102 70-71 -0.07433729893889307 1102 72-73 0.02918274121370601 1102 74-75 0.13034804503771724 1102 76-77 0.016165978691791594 1102 78-79 -0.03327421744106118 1102 80-81 0.059498330296829494 1102 82-83 -0.13475909503535632 1102 84-85 -0.10656761170751494 1102 86-87 -0.22059989530009005 1102 88-89 -0.017992856544029934 1102 90-91 0.06908911149161767 1102 92-93 0.02617898038982247 1102 94-95 -0.11587426690504188 1102 96-97 -0.055999806192708945 1102 98-99 0.28135732255200097 1102 100-101 0.6695382065884843 1103 1 0.24891073130818597 1103 2 0.21737233196051875 1103 3 0.18763870119580162 1103 4 0.14251537949775184 1103 5 0.12631811615175081 1103 6 0.15999007910312457 1103 7 0.10918279128539154 1103 8 0.04501743171233841 1103 9 0.21839033728695512 1103 10-11 0.2068697833792683 1103 12-13 0.2855402213334344 1103 14-15 0.3373727291191315 1103 16-17 0.2762591819726907 1103 18-19 0.3241703572467216 1103 20-21 0.2211805843796526 1103 22-23 0.2885694492138029 1103 24-25 0.25981070239243564 1103 26-27 0.3489126408164438 1103 28-29 0.2563496241053471 1103 30-31 0.2955375312608197 1103 32-33 0.3458683905247071 1103 34-35 0.3357614629620116 1103 36-37 0.23516349378806467 1103 38-39 0.16836986709565593 1103 40-41 0.2916848602197888 1103 42-43 0.2945217655952419 1103 44-45 0.14141690291547349 1103 46-47 0.17803313841761081 1103 48-49 0.32144969684978975 1103 50-51 0.3682017012162433 1103 52-53 0.4971802549963229 1103 54-55 0.4592478788630032 1103 56-57 0.3595856676902329 1103 58-59 0.4022829722195027 1103 60-61 0.24154753219553982 1103 62-63 0.5220862572555944 1103 64-65 0.5252428655676269 1103 66-67 0.5248744317932221 1103 68-69 0.3414116945852257 1103 70-71 0.25450603802638483 1103 72-73 0.4620041917798119 1103 74-75 0.4548449894690023 1103 76-77 0.2607618382777517 1103 78-79 0.36399813084640087 1103 80-81 0.41697703606214276 1103 82-83 0.3873510949310095 1103 84-85 0.3293479693242709 1103 86-87 0.23974327585913358 1103 88-89 0.2155386229197056 1103 90-91 0.20405571341496653 1103 92-93 0.14815984163384144 1103 94-95 0.2021665687574732 1103 96-97 0.31013198593387514 1103 98-99 -0.0022714613790206784 1103 100-101 0.31494774754257904 1104 1 0.15923154229301417 1104 2 0.18042262132846787 1104 3 0.0873806560453616 1104 4 0.03230286123993409 1104 5 0.019359976763418274 1104 6 -0.030830453894445498 1104 7 0.1255749187523989 1104 8 0.060539527450238495 1104 9 0.13152833464265257 1104 10-11 0.146382884850631 1104 12-13 0.1498088453400115 1104 14-15 0.1119285817564375 1104 16-17 0.13174000872879077 1104 18-19 0.1456836693333443 1104 20-21 0.10596522820561916 1104 22-23 0.0746528594894258 1104 24-25 0.06873374117259345 1104 26-27 -0.07948729268220234 1104 28-29 -0.07495398069087855 1104 30-31 0.037845824734105804 1104 32-33 -0.07145610332495522 1104 34-35 0.010669660829236705 1104 36-37 -0.07207037331122024 1104 38-39 -0.046435100273491514 1104 40-41 -0.029679093310527094 1104 42-43 -0.09044177682071819 1104 44-45 -0.14110004062595038 1104 46-47 -0.03582903545522953 1104 48-49 -0.11274878978836966 1104 50-51 0.017061186427774544 1104 52-53 -0.08894998400638343 1104 54-55 -0.13941085849852897 1104 56-57 -0.15922221263718228 1104 58-59 -0.1364368601506456 1104 60-61 -0.02319107892950001 1104 62-63 -0.07580029326035032 1104 64-65 -0.03716765922506937 1104 66-67 -0.08644215817991352 1104 68-69 -0.011583934318984745 1104 70-71 -0.07613991233557726 1104 72-73 0.03807527194172522 1104 74-75 0.08776073383017291 1104 76-77 0.03258974654071167 1104 78-79 0.2014803705489534 1104 80-81 0.38465889176498536 1104 82-83 0.38650842660153373 1104 84-85 0.38942773363418937 1104 86-87 0.34450529512876926 1104 88-89 0.24004918083869242 1104 90-91 0.1629910242652599 1104 92-93 0.1684584053455751 1104 94-95 0.4594745657939363 1104 96-97 0.47880433451373605 1104 98-99 0.40620107633997193 1104 100-101 0.7945036602964528 1105 1 0.24229843025377562 1105 2 0.25135655029450277 1105 3 0.20164632450571673 1105 4 0.15998015672217747 1105 5 0.18246545855802765 1105 6 0.1881918011786965 1105 7 0.17621314000108868 1105 8 0.1952182497443573 1105 9 0.20086010695791856 1105 10-11 0.1600190880608352 1105 12-13 0.13146317380437722 1105 14-15 0.20720890746610365 1105 16-17 0.14809102108632288 1105 18-19 0.14747904243441923 1105 20-21 0.22587508750492447 1105 22-23 0.1938753897639387 1105 24-25 0.13741471462381583 1105 26-27 0.18843041397004612 1105 28-29 0.2197224621212115 1105 30-31 0.29120137290121306 1105 32-33 0.14477569706794924 1105 34-35 0.3847767231996002 1105 36-37 0.31820909703067457 1105 38-39 0.3283561439078184 1105 40-41 0.4134435587836407 1105 42-43 0.39532404933752474 1105 44-45 0.35582985226312047 1105 46-47 0.5115913844325561 1105 48-49 0.6133417703909672 1105 50-51 0.6969330004076539 1105 52-53 0.6131712830888816 1105 54-55 0.6366080014052713 1105 56-57 0.5932920178274443 1105 58-59 0.6071173648827894 1105 60-61 0.6782759048864051 1105 62-63 0.751997315711364 1105 64-65 0.6117237684163257 1105 66-67 0.5550171239540944 1105 68-69 0.5849579745426539 1105 70-71 0.43538338637615226 1105 72-73 0.36604159405591474 1105 74-75 0.5327729506399344 1105 76-77 0.4694426990431815 1105 78-79 0.6122023436542108 1105 80-81 0.6947199274815894 1105 82-83 0.7648380383544868 1105 84-85 0.7623036733866257 1105 86-87 0.5338928225854005 1105 88-89 0.5212520639563429 1105 90-91 0.6590390228732907 1105 92-93 0.6106359940807096 1105 94-95 0.444910608900873 1105 96-97 0.20488185623569066 1105 98-99 -0.010789809553518381 1105 100-101 0.07885982689107252 1106 1 0.13599541853259467 1106 2 0.0708206509957563 1106 3 0.06743525444748855 1106 4 -0.0019745626679394945 1106 5 0.059366807970853586 1106 6 0.07366907978012449 1106 7 0.06702038081066064 1106 8 0.09112735378193548 1106 9 0.0707962910841573 1106 10-11 0.10896268614450833 1106 12-13 0.12025373820864615 1106 14-15 0.13630937543929633 1106 16-17 0.04392532643235114 1106 18-19 -0.11105708051982077 1106 20-21 0.0540480517030133 1106 22-23 0.04518558882472945 1106 24-25 0.09137982288567059 1106 26-27 0.1333326609671701 1106 28-29 0.14814714983667443 1106 30-31 0.007361445063423844 1106 32-33 0.012760079693627802 1106 34-35 -0.01970090421552584 1106 36-37 -3.5038385159680274E-4 1106 38-39 0.09938846212663321 1106 40-41 0.0716953726519094 1106 42-43 -0.09644179965354027 1106 44-45 -0.18516575535034718 1106 46-47 -0.12717499999865112 1106 48-49 -0.26314078040734046 1106 50-51 -0.21660082304224915 1106 52-53 -0.29607198373285826 1106 54-55 -0.2765529394585897 1106 56-57 -0.09480965803353314 1106 58-59 -0.03271841866312997 1106 60-61 -0.23027195627834374 1106 62-63 0.034291650784318506 1106 64-65 -0.1614317958287117 1106 66-67 -0.05857283554352932 1106 68-69 -0.18896816434148178 1106 70-71 -0.31224619865403014 1106 72-73 -0.3059317065141087 1106 74-75 0.0021658444680063837 1106 76-77 -0.07403533643213223 1106 78-79 -0.029858515819785936 1106 80-81 -0.12657637087187368 1106 82-83 -0.0726269969601212 1106 84-85 -0.009295189377031221 1106 86-87 -0.2925060795771728 1106 88-89 -0.2368248987131878 1106 90-91 -0.147924439076764 1106 92-93 -0.19812930169627307 1106 94-95 -0.15503599520150146 1106 96-97 -0.23334368181499698 1106 98-99 -0.22096783784519758 1106 100-101 -0.10962213736700122 1107 1 0.08457175880089807 1107 2 0.10040971871077176 1107 3 0.03904652537590181 1107 4 0.0718087914644272 1107 5 0.02881908053389992 1107 6 0.014476541262851583 1107 7 0.09343686341075141 1107 8 0.0503241399893426 1107 9 0.07919914982844034 1107 10-11 0.1509603796975867 1107 12-13 0.14539029900529243 1107 14-15 0.1689800935235226 1107 16-17 0.1462042121899998 1107 18-19 0.08989174116117482 1107 20-21 0.12218197795530727 1107 22-23 0.008443801513898563 1107 24-25 0.05527958325735938 1107 26-27 0.07637650689417086 1107 28-29 0.05472555243176913 1107 30-31 0.10307118037863461 1107 32-33 0.11853549796904161 1107 34-35 0.14303164461338014 1107 36-37 0.18946350873164164 1107 38-39 0.22388374377289466 1107 40-41 0.37262236999392684 1107 42-43 0.2611413281306483 1107 44-45 0.33880464362004403 1107 46-47 0.2650692792903868 1107 48-49 0.32924989771929347 1107 50-51 0.4050571724776546 1107 52-53 0.3399596825529798 1107 54-55 0.4957171628570407 1107 56-57 0.4805800065063508 1107 58-59 0.5122779244174254 1107 60-61 0.5329290834780878 1107 62-63 0.38582731282833294 1107 64-65 0.40885453754562917 1107 66-67 0.3972223808738491 1107 68-69 0.5242791850022215 1107 70-71 0.5986714391224766 1107 72-73 0.4431396605390461 1107 74-75 0.4455332716176805 1107 76-77 0.42065053467634783 1107 78-79 0.5890775403317505 1107 80-81 0.8273894093458019 1107 82-83 0.808896869199554 1107 84-85 0.5942854669350375 1107 86-87 0.46717108620773473 1107 88-89 0.49824703372217627 1107 90-91 0.6092745412823106 1107 92-93 0.6048771108511097 1107 94-95 0.48861282921363625 1107 96-97 0.644517161903206 1107 98-99 0.6002777655513931 1107 100-101 0.6010536506039266 1108 1 0.10252499859986841 1108 2 0.08672708409417851 1108 3 0.06855732534013015 1108 4 0.1673477050171428 1108 5 0.1656035626855612 1108 6 0.1700367959482918 1108 7 0.07070494152900153 1108 8 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5 33.0 6 92.0 7 209.0 8 474.0 9 1057.0 10 1777.0 11 3026.0 12 5055.0 13 7075.0 14 8974.0 15 12550.0 16 17840.0 17 25372.0 18 22441.0 19 12091.0 20 9644.0 21 9891.0 22 10093.0 23 10841.0 24 12646.0 25 15090.0 26 18068.0 27 22689.0 28 28686.0 29 35463.0 30 44273.0 31 56109.0 32 72026.0 33 95162.0 34 131203.0 35 189089.0 36 291706.0 37 443357.0 38 319288.0 39 19521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.438248603630946 20.76439841527158 15.467799639927577 21.329553341169902 2 15.001863967259723 22.082481575400546 40.30609005215012 22.609564405189612 3 18.428795415280284 27.42526327216346 30.699177949759388 23.446763362796876 4 12.364676857912109 18.31044621433158 38.81350881091782 30.511368116838494 5 12.640481657248312 39.14842564277975 34.04341387580879 14.167678824163152 6 29.178320120680457 38.69665312459356 17.968115535355665 14.156911219370318 7 26.91013381971462 34.61360031050844 21.61024022415596 16.86602564562098 8 26.109437838402478 34.73710729438801 20.23430483285081 18.9191500343587 9 27.018497894184364 16.919748582254822 21.174289847280363 34.88746367628045 10-11 23.95919831557471 27.98618435320901 27.732358075058116 20.322259256158162 12-13 25.66615427312658 25.723760142473395 28.22982027992868 20.380265304471344 14-15 22.63680241852482 26.471561134420863 27.539445939067843 23.352190507986478 16-17 20.661663575578388 30.008791935792757 27.8585824552492 21.470962033379656 18-19 20.527998287694906 27.911289897246334 31.34813814984001 20.212573665218752 20-21 21.68018585664521 26.900619231984386 31.29520513819683 20.123989773173577 22-23 21.36695569175277 27.11278225519449 31.120387405927737 20.399874647125 24-25 21.10415165570002 27.22227599284902 31.476812825472322 20.19675952597864 26-27 21.03446766117182 27.252160389752206 31.447455373110976 20.26591657596499 28-29 20.551353870102208 27.16716506564529 31.789243799029148 20.49223726522336 30-31 20.871061780006052 27.15966820302927 31.54293579588311 20.42633422108157 32-33 20.840390354060812 27.41327065546761 31.31394364880936 20.432395341662218 34-35 21.14434955548751 27.114545046765727 31.28001265792969 20.461092739817076 36-37 21.397441743815403 26.765954406945397 31.131100323616312 20.70550352562289 38-39 20.948833188574426 27.31157613772308 31.1752562642369 20.564334409465594 40-41 21.011652131479035 27.35832704569015 31.27441715659931 20.355603666231502 42-43 21.044678600213217 27.548483659903138 30.57156287539825 20.835274864485392 44-45 21.07325111140924 27.507852320057186 30.384049370022215 21.03484719851136 46-47 21.561118335334186 27.81429146757382 29.686666712751492 20.937923484340505 48-49 21.560710483612045 28.090443435216017 29.306732102083753 21.04211397908818 50-51 21.532968849176225 28.07811633058182 29.120206799909155 21.2687080203328 52-53 21.587095465982838 28.452178935607392 28.87661898095473 21.08410661745504 54-55 21.54436053957426 28.217631151641708 28.916217286019112 21.32179102276492 56-57 21.530867965339148 28.553367204918313 28.58790618506339 21.327858644679154 58-59 21.71667246566494 28.121173205197238 28.835819733850304 21.326334595287527 60-61 21.62044388413726 28.262371236168626 29.063770388300046 21.05341449139407 62-63 21.648409439819257 28.181171569114426 29.22708740220623 20.943331588860087 64-65 21.555450641754987 28.200966140038076 29.04406004118948 21.19952317701746 66-67 21.529069495035667 28.48274638412335 28.594529977640722 21.393654143200262 68-69 21.512800568993832 28.26591551834406 28.95992720647686 21.261356706185246 70-71 21.582488651521707 28.258696659591738 28.96467058049419 21.19414410839237 72-73 21.60463325287287 28.238473961635002 28.668700196525627 21.488192588966506 74-75 21.559931098259742 28.73211785802432 28.322322504795366 21.385628538920574 76-77 21.53028327750258 28.523200571859864 28.58912728900122 21.357388861636334 78-79 21.517606391980685 28.35369936112453 28.431891389378123 21.696802857516666 80-81 21.59329135093672 28.854841203923225 28.079602351806205 21.472265093333853 82-83 21.691441751706993 28.82771095011474 28.146244611950323 21.33460268622795 84-85 21.45956219637689 28.64079521889541 28.10499236853486 21.79465021619284 86-87 21.350399444500486 28.598572231066903 28.241681205343816 21.80934711908879 88-89 21.533832977158106 28.372755833622858 28.222545213601446 21.870865975617594 90-91 21.42363374470214 28.400014132748495 28.453472790106666 21.722879332442695 92-93 21.37843049703931 28.58918216991547 28.24974243749373 21.782644895551492 94-95 21.162609633598414 28.32130565144446 28.262650060127744 22.25343465482938 96-97 21.4152358333807 28.373614804368717 28.088293051862294 22.122856310388283 98-99 21.140985047044346 28.467838587038756 27.973929939272104 22.417246426644798 100-101 20.79656575970047 28.848657984629178 27.91354591404363 22.44123034162672 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 858.0 1 627.5 2 430.0 3 459.0 4 446.0 5 485.5 6 785.0 7 1589.0 8 2681.0 9 3295.5 10 3104.0 11 2866.5 12 2905.5 13 3015.5 14 3321.5 15 3610.5 16 3754.5 17 3982.5 18 4231.0 19 4536.5 20 5034.0 21 5627.5 22 6462.5 23 7563.5 24 8745.0 25 10733.5 26 13899.5 27 16872.5 28 18958.5 29 22139.0 30 27094.0 31 32041.5 32 37997.5 33 44921.0 34 52593.5 35 58395.5 36 65017.0 37 71539.5 38 72220.0 39 71126.5 40 69451.5 41 67973.5 42 66656.0 43 68015.5 44 71037.5 45 71905.5 46 69335.5 47 67483.0 48 73117.0 49 70324.5 50 59793.0 51 53966.5 52 50061.0 53 48593.5 54 46866.0 55 43942.5 56 40283.0 57 36284.0 58 33736.5 59 30946.5 60 26897.5 61 22097.5 62 17716.5 63 14094.0 64 10409.5 65 7706.5 66 5677.0 67 4407.5 68 2999.5 69 1698.0 70 1201.5 71 817.0 72 578.0 73 441.0 74 322.5 75 229.5 76 155.5 77 74.0 78 34.5 79 21.0 80 13.0 81 9.5 82 9.5 83 3.5 84 2.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027702022503668856 2 0.005222932154111319 3 2.0482086878867913E-4 4 4.0964173757735827E-4 5 1.0241043439433957E-4 6 0.0 7 0.0 8 0.0 9 5.1205217197169784E-5 10-11 0.01088110865439858 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.5361565159150936E-4 20-21 0.0011777199955349052 22-23 0.0019970034706896216 24-25 0.009575375615870749 26-27 0.004890098242329714 28-29 3.072313031830187E-4 30-31 0.00230423477387264 32-33 0.0042756356359636765 34-35 0.0 36-37 0.007706385188174052 38-39 0.013006125168081126 40-41 0.0011009121697391504 42-43 0.0 44-45 0.0 46-47 2.8162869458443383E-4 48-49 0.003021107814633017 50-51 0.006170228672258958 52-53 0.0 54-55 0.0027138765114499987 56-57 0.003072313031830187 58-59 1.2801304299292447E-4 60-61 0.0014849512987179239 62-63 0.004710879982139621 64-65 0.0 66-67 0.0023298373824712252 68-69 2.5602608598584894E-4 70-71 0.0010753095611405654 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002125016513682546 80-81 0.0022530295566754706 82-83 0.0115211738693632 84-85 0.0079624112741599 86-87 0.004557264330548111 88-89 7.680782579575468E-5 90-91 7.680782579575468E-4 92-93 1.0241043439433957E-4 94-95 2.5602608598584892E-5 96-97 0.0022018243394783007 98-99 0.0017665799933023576 100-101 2.5602608598584894E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1952926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.768136392507714 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6088765760105 32.953322262541036 2 14.00859645206673 12.542775132674459 3 4.880473674789856 6.554691333991068 4 2.3358031991039137 4.182782248141594 5 1.2665362899412431 2.8350234687075133 6 0.7918971293719552 2.1271055217935406 7 0.5072473512342676 1.5895963023355768 8 0.3687294222212402 1.3205863252744838 9 0.28538094486871934 1.1498375757334998 >10 1.6324050708810205 13.963420500704382 >50 0.1810578973036627 5.663793005669394 >100 0.12304786921391636 10.240795597200645 >500 0.008347275614871254 2.4782944520685843 >1k 0.0012578086542956684 0.8698320560500152 >5k 2.2869248259921245E-4 0.8504307878506886 >10k+ 1.1434624129960622E-4 0.6777134292635447 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13239 0.6779058704733308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9882 0.5060099563424317 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6731 0.34466231695414984 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2860 0.14644692118390557 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2348 0.12022984997895467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0241043439433957E-4 0.0 16-17 0.0 0.0 0.0 1.5361565159150936E-4 0.0 18-19 0.0 0.0 0.0 1.5361565159150936E-4 0.0 20-21 0.0 0.0 0.0 2.3042347738726405E-4 0.0 22-23 0.0 0.0 0.0 4.8644956337311294E-4 0.0 24-25 0.0 0.0 0.0 7.936808665561317E-4 0.0 26-27 0.0 0.0 0.0 0.0013569382557249991 0.0 28-29 0.0 0.0 0.0 0.0025858634684570742 0.0 30-31 0.0 0.0 0.0 0.00611902345506179 0.0 32-33 0.0 0.0 0.0 0.011290750391975938 0.0 34-35 0.0 0.0 0.0 0.015822412113925464 0.0 36-37 0.0 0.0 0.0 0.01866430166836839 0.0 38-39 0.0 0.0 0.0 0.02109654948523395 0.0 40-41 0.0 0.0 0.0 0.02506495381801461 0.0 42-43 0.0 0.0 0.0 0.0293149868453797 2.5602608598584892E-5 44-45 0.0 0.0 0.0 0.03384664856732923 5.1205217197169784E-5 46-47 0.0 0.0 0.0 0.04073375028034856 5.1205217197169784E-5 48-49 0.0 0.0 0.0 0.048542545902916956 5.1205217197169784E-5 50-51 0.0 0.0 0.0 0.05870678151655516 5.1205217197169784E-5 52-53 0.0 0.0 0.0 0.08177473186388015 5.1205217197169784E-5 54-55 0.0 0.0 0.0 0.11096170566626692 5.1205217197169784E-5 56-57 0.0 0.0 0.0 0.14565324031734944 5.1205217197169784E-5 58-59 0.0 0.0 0.0 0.19365813143969612 5.1205217197169784E-5 60-61 0.0 0.0 0.0 0.2609673894453758 5.1205217197169784E-5 62-63 0.0 0.0 0.0 0.3057207492757022 5.1205217197169784E-5 64-65 0.0 0.0 0.0 0.34916837606750073 7.680782579575468E-5 66-67 0.0 0.0 0.0 0.4445892983144267 1.0241043439433957E-4 68-69 0.0 0.0 0.0 0.5657920474201275 1.0241043439433957E-4 70-71 0.0 0.0 0.0 0.6545306888228228 1.0241043439433957E-4 72-73 0.0 0.0 0.0 0.7370222937274633 1.0241043439433957E-4 74-75 0.0 0.0 0.0 0.9086109765551793 1.0241043439433957E-4 76-77 0.0 0.0 0.0 1.0289944421857253 1.0241043439433957E-4 78-79 0.0 0.0 0.0 1.120472562708469 1.0241043439433957E-4 80-81 0.0 0.0 0.0 1.2418545300743602 1.0241043439433957E-4 82-83 0.0 0.0 0.0 1.4658517527033794 1.0241043439433957E-4 84-85 0.0 0.0 0.0 1.701779790939339 1.0241043439433957E-4 86-87 0.0 0.0 0.0 2.0198153949509607 1.0241043439433957E-4 88-89 0.0 0.0 0.0 2.4234917247248484 1.0241043439433957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16830 0.0 29.755486 1 TATCGCG 85 6.4063835E-4 27.942976 7 GGTATCA 11420 0.0 27.625652 1 GTACATG 11795 0.0 24.854086 1 ATCAACG 20515 0.0 24.16978 3 TCAACGC 20630 0.0 24.058071 4 TATCAAC 20705 0.0 24.018032 2 CAACGCA 20715 0.0 23.986582 5 AACGCAG 21015 0.0 23.666765 6 TACATGG 12530 0.0 23.12303 2 ACATGGG 12075 0.0 22.695116 3 AGTACTT 11590 0.0 22.087627 12-13 GAGTACT 10935 0.0 22.020792 12-13 TACCGTG 110 0.0028770033 21.5923 7 CATTCCG 355 0.0 21.40983 9 ACGCAGA 23180 0.0 21.394821 7 CATATAG 690 0.0 21.338127 3 CGCAGAG 23255 0.0 21.203257 8 CATGGGA 7800 0.0 20.763643 4 GTACTTT 11730 0.0 20.325884 14-15 >>END_MODULE