FastQCFastQC Report
Thu 26 May 2016
SRR937040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994957
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133580.6695883670675609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89670.44948337232331326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60240.30196139565915453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21930.10992718138786951No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.10310999184443574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141600.033.7030371
GGTATCA91650.030.651631
GTATAGG8350.027.8938311
ATCAACG173300.027.3755743
TCAACGC174100.027.2770864
CAACGCA175500.027.0039635
TATCAAC177450.026.9228692
AACGCAG179400.026.46466
GTACATG120200.025.7439421
TACATGG125850.024.1745262
ACGCAGA197950.023.8867557
GAGTACT92100.023.8366212-13
ACATGGG123950.023.6630043
CGCAGAG198850.023.5874588
CGCGGAA2700.022.87093782-83
GCAGAGT203800.022.4304879
AGTACTT97700.022.12988512-13
GTACTTT98550.022.10860814-15
CATGGGG41700.021.3175264
CATGGGA81100.020.2222524
Produced by FastQC (version 0.11.5)