##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994957 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795331929460133 33.0 31.0 34.0 30.0 34.0 2 31.876311118485262 33.0 31.0 34.0 30.0 34.0 3 31.886643170755057 33.0 31.0 34.0 30.0 34.0 4 35.4987711514584 37.0 35.0 37.0 33.0 37.0 5 35.51180401382085 37.0 35.0 37.0 33.0 37.0 6 35.590109962269864 37.0 35.0 37.0 33.0 37.0 7 35.53935147474357 37.0 35.0 37.0 33.0 37.0 8 35.50285043737785 37.0 35.0 37.0 33.0 37.0 9 37.11803412304125 39.0 37.0 39.0 33.0 39.0 10-11 36.99481993847486 39.0 37.0 39.0 33.0 39.0 12-13 36.914260307365026 39.0 37.0 39.0 33.0 39.0 14-15 38.03998883183948 40.0 38.0 41.0 33.0 41.0 16-17 37.98049983032216 40.0 38.0 41.0 33.0 41.0 18-19 37.987697228561814 40.0 38.0 41.0 33.0 41.0 20-21 37.94507250030953 40.0 38.0 41.0 33.0 41.0 22-23 37.831151749135444 40.0 37.5 41.0 33.0 41.0 24-25 37.78897690526663 40.0 37.5 41.0 32.5 41.0 26-27 37.804674737350226 40.0 37.0 41.0 33.0 41.0 28-29 37.730156088577345 40.0 37.0 41.0 32.0 41.0 30-31 37.555091162365905 40.0 37.0 41.0 32.0 41.0 32-33 37.34707214240708 40.0 36.5 41.0 32.0 41.0 34-35 37.1741428511993 40.0 36.0 41.0 31.0 41.0 36-37 36.990972487126285 39.0 36.0 41.0 31.0 41.0 38-39 36.826088732739606 39.0 36.0 41.0 30.5 41.0 40-41 36.637123506922705 39.0 35.0 41.0 30.0 41.0 42-43 36.39761007380109 39.0 35.0 41.0 30.0 41.0 44-45 36.098802881465616 39.0 35.0 41.0 29.5 41.0 46-47 35.68988178692574 39.0 35.0 40.0 27.5 41.0 48-49 35.38895901014408 39.0 35.0 40.0 27.0 41.0 50-51 34.68011039836949 38.0 34.0 40.0 25.0 40.5 52-53 34.85633600122709 38.5 34.5 40.0 26.0 40.5 54-55 35.24731009239798 39.0 35.0 40.5 26.0 41.0 56-57 35.14998518765066 39.0 35.0 41.0 26.0 41.0 58-59 34.944503816372986 39.0 35.0 40.5 26.0 41.0 60-61 34.679048220086955 38.0 34.0 40.0 25.5 41.0 62-63 34.38285662297483 38.0 34.0 40.0 24.5 41.0 64-65 34.065553041995386 37.5 34.0 40.0 23.5 41.0 66-67 33.704684862881756 37.0 34.0 40.0 22.5 41.0 68-69 33.32088260548974 36.5 33.0 39.0 22.0 41.0 70-71 32.91586209627576 36.0 33.0 39.0 21.0 41.0 72-73 32.537365216393134 35.5 32.5 38.5 20.0 40.0 74-75 32.14213138428548 35.0 32.0 37.5 19.0 40.0 76-77 31.68681355036725 35.0 32.0 37.0 17.5 39.0 78-79 31.22955206553324 35.0 31.0 36.5 13.0 39.0 80-81 30.797701153458448 35.0 31.0 36.0 9.0 38.5 82-83 30.39904318739702 35.0 31.0 36.0 7.0 37.0 84-85 30.030527224396316 34.0 30.5 35.0 4.0 37.0 86-87 29.692468559472708 34.0 30.0 35.0 2.0 36.5 88-89 29.330724170997172 34.0 30.0 35.0 2.0 36.0 90-91 28.974035279958414 34.0 29.0 35.0 2.0 36.0 92-93 28.62176127104494 34.0 29.0 35.0 2.0 35.0 94-95 28.270243168148486 34.0 29.0 35.0 2.0 35.0 96-97 27.80987108995332 34.0 29.0 35.0 2.0 35.0 98-99 27.280945403835773 34.0 27.0 35.0 2.0 35.0 100-101 25.096296311148564 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29533626116428024 1101 2 0.21640886548057026 1101 3 0.266578201516932 1101 4 0.261325804312591 1101 5 0.2798112541047999 1101 6 0.15526982638456133 1101 7 0.1970365572776629 1101 8 0.34743894126066976 1101 9 0.22165324177166212 1101 10-11 0.2826137479750912 1101 12-13 0.3475448612416443 1101 14-15 0.4260851533762633 1101 16-17 0.4608408661722123 1101 18-19 0.5547627220654405 1101 20-21 0.5315141247594042 1101 22-23 0.48625368643912026 1101 24-25 0.5687400252445229 1101 26-27 0.5069156421711014 1101 28-29 0.4831428936209008 1101 30-31 0.5175173489277256 1101 32-33 0.40215078596751397 1101 34-35 0.4233327295528113 1101 36-37 0.5283023405297413 1101 38-39 0.43812119069362865 1101 40-41 0.4923951088032368 1101 42-43 0.4838214787072417 1101 44-45 0.6950436892650558 1101 46-47 0.4824530697970886 1101 48-49 0.5470938202225639 1101 50-51 0.5399884753381343 1101 52-53 0.4864572252067063 1101 54-55 0.5628771685512959 1101 56-57 0.5700391128227764 1101 58-59 0.5859208148542905 1101 60-61 0.6420699673759458 1101 62-63 0.6650216362741901 1101 64-65 0.6388580016277388 1101 66-67 0.4517575632260531 1101 68-69 0.3911954975507328 1101 70-71 0.4340245104960019 1101 72-73 0.5973858746558491 1101 74-75 0.6058338333294344 1101 76-77 0.5703105749063333 1101 78-79 0.6076500325870171 1101 80-81 0.6173232464131253 1101 82-83 0.4534754936138192 1101 84-85 0.44673812312402816 1101 86-87 0.6032684057425222 1101 88-89 0.4193164376658949 1101 90-91 0.35082341046844334 1101 92-93 0.4398274911607345 1101 94-95 0.4104629958785857 1101 96-97 0.5195302004663596 1101 98-99 0.6155669136741118 1101 100-101 0.9545034561021097 1102 1 0.1362508028202356 1102 2 0.18428105818093954 1102 3 0.1482860214152204 1102 4 0.2058021351182049 1102 5 0.2065626943702341 1102 6 0.15449137544595004 1102 7 0.12674418684907351 1102 8 0.06681909838880529 1102 9 0.2513005020354484 1102 10-11 0.24131613848421551 1102 12-13 0.09189466315648787 1102 14-15 -0.08473907572552264 1102 16-17 0.17987142010915136 1102 18-19 0.149413522094342 1102 20-21 0.19818274484228482 1102 22-23 0.1169100290387064 1102 24-25 0.11894617479701708 1102 26-27 0.1542965221215411 1102 28-29 -0.04686280394023612 1102 30-31 0.007932256280696492 1102 32-33 -0.028008432614683443 1102 34-35 0.03272835248937156 1102 36-37 0.17702565475050136 1102 38-39 0.2413708647500883 1102 40-41 0.09239010572529338 1102 42-43 0.16128616371187832 1102 44-45 0.23794823141258092 1102 46-47 0.3031611242802583 1102 48-49 0.35706170081203226 1102 50-51 0.34795932313767963 1102 52-53 0.47849349717192524 1102 54-55 0.30532711284395475 1102 56-57 0.43903517875914133 1102 58-59 0.2666211443052191 1102 60-61 0.28397746256398193 1102 62-63 0.15250204598778794 1102 64-65 0.3189831520480766 1102 66-67 0.04550912122126505 1102 68-69 0.29136003933788857 1102 70-71 0.3086513132440558 1102 72-73 0.3204857152745859 1102 74-75 0.3492081225118895 1102 76-77 0.2787545459437659 1102 78-79 0.36778314594819506 1102 80-81 0.39000358944234037 1102 82-83 0.4294503984547511 1102 84-85 0.4011769640823033 1102 86-87 0.34189449406504124 1102 88-89 0.3625408619227066 1102 90-91 0.3727358422599565 1102 92-93 0.38946276502939625 1102 94-95 0.47008927645224574 1102 96-97 0.1812535578955199 1102 98-99 0.37170946011133665 1102 100-101 0.23324775701031086 1103 1 0.2620113545921754 1103 2 0.3189170710099809 1103 3 0.3010551566722448 1103 4 0.2345795810390925 1103 5 0.12815613491776645 1103 6 0.22560605415780088 1103 7 0.23734346978041998 1103 8 0.23703751288942954 1103 9 0.18086537011100035 1103 10-11 0.23210000895070948 1103 12-13 0.24524685528563595 1103 14-15 0.520216800277602 1103 16-17 0.5612513010846385 1103 18-19 0.42568205120135616 1103 20-21 0.31806358785265587 1103 22-23 0.3453977903939034 1103 24-25 0.4006015801298446 1103 26-27 0.4852603328537981 1103 28-29 0.5548970002586273 1103 30-31 0.4667433250882027 1103 32-33 0.406290080330713 1103 34-35 0.3999428336385691 1103 36-37 0.37427288160527183 1103 38-39 0.3190663705040393 1103 40-41 0.33134192091721104 1103 42-43 0.3339358913561199 1103 44-45 0.3068854424351386 1103 46-47 0.26359095015444467 1103 48-49 0.3721560174665157 1103 50-51 0.4208611239724718 1103 52-53 0.4529681552324263 1103 54-55 0.39749288359250556 1103 56-57 0.3676190361932399 1103 58-59 0.49354206339046414 1103 60-61 0.5337077741458529 1103 62-63 0.46560931484700063 1103 64-65 0.4694948887304946 1103 66-67 0.4271466965066679 1103 68-69 0.5032334545939676 1103 70-71 0.4187253616458335 1103 72-73 0.278448424697082 1103 74-75 0.3905826043485163 1103 76-77 0.3906855329192389 1103 78-79 0.5231069367451688 1103 80-81 0.3465134006228574 1103 82-83 0.3524781195240685 1103 84-85 0.35830536691821635 1103 86-87 0.2933207725474709 1103 88-89 0.22278291734766142 1103 90-91 0.24988421976144792 1103 92-93 0.28126624210346307 1103 94-95 0.4447697140259592 1103 96-97 0.45614983571200796 1103 98-99 0.3406546552715888 1103 100-101 0.46243162418289785 1104 1 0.12678281181656104 1104 2 0.029624295975676063 1104 3 0.09989811354644118 1104 4 0.08606827159792374 1104 5 0.2228530722979869 1104 6 0.029758782609441425 1104 7 0.0852362753734397 1104 8 0.03049488308661097 1104 9 0.1399857132993958 1104 10-11 0.2101663710126047 1104 12-13 0.1042197177457993 1104 14-15 -0.02325773011475718 1104 16-17 0.1940679154110967 1104 18-19 0.14112161202445606 1104 20-21 0.10040435093647915 1104 22-23 -2.2948163532987564E-4 1104 24-25 0.08360536661901108 1104 26-27 -0.05366344950915902 1104 28-29 0.04664381893295655 1104 30-31 0.1593717512132713 1104 32-33 -0.006088896665701782 1104 34-35 -0.03891374280748039 1104 36-37 -0.021100341975198944 1104 38-39 0.15599584733720206 1104 40-41 0.15519280832228333 1104 42-43 0.2762342988124473 1104 44-45 0.10844387900755947 1104 46-47 0.16501968667545697 1104 48-49 0.19492746073006373 1104 50-51 0.05991939572946592 1104 52-53 0.2806234676022683 1104 54-55 0.35930747437323873 1104 56-57 0.3675209225349576 1104 58-59 0.3295684485963193 1104 60-61 0.3575988959068468 1104 62-63 0.20118156566739032 1104 64-65 0.2006103363188032 1104 66-67 0.009195406641694603 1104 68-69 -0.03268060404619888 1104 70-71 0.04018386577320143 1104 72-73 0.13735022262017793 1104 74-75 0.058940018832572605 1104 76-77 0.18503174305620362 1104 78-79 0.3613296330325966 1104 80-81 0.33779573062446033 1104 82-83 0.2987484819861699 1104 84-85 0.1563924996027808 1104 86-87 0.4109511958477974 1104 88-89 0.30474921976575686 1104 90-91 0.35778859345892045 1104 92-93 0.25101892588042674 1104 94-95 0.464597398520354 1104 96-97 0.4092975584280687 1104 98-99 0.38459047497823917 1104 100-101 0.40959034183350695 1105 1 0.115258383454659 1105 2 0.26523953038421766 1105 3 0.2308847710307198 1105 4 0.1991078371632753 1105 5 0.21475873011544167 1105 6 0.14032228020679582 1105 7 0.23579726556018699 1105 8 0.20834178162579065 1105 9 0.17793970918449276 1105 10-11 0.21389421220077054 1105 12-13 0.14223478882917817 1105 14-15 0.30415749879809084 1105 16-17 0.3423724507329524 1105 18-19 0.17478767817201657 1105 20-21 0.13512640618882443 1105 22-23 0.2676328134353767 1105 24-25 0.13365396916695715 1105 26-27 0.3576151109280232 1105 28-29 0.19481332332436097 1105 30-31 0.22213988099409931 1105 32-33 0.18724448246508274 1105 34-35 0.017431616078546597 1105 36-37 0.1421451833273153 1105 38-39 0.39307194896633035 1105 40-41 0.3835187339174553 1105 42-43 0.5222620494933992 1105 44-45 0.31851229181665275 1105 46-47 0.4065996816606301 1105 48-49 0.3168867960258197 1105 50-51 0.2090766223959477 1105 52-53 0.5203493097315786 1105 54-55 0.3897770213562666 1105 56-57 0.4770736202339805 1105 58-59 0.4926249520637569 1105 60-61 0.5118941675487179 1105 62-63 0.5995075685457678 1105 64-65 0.5955863270036019 1105 66-67 0.6165794517819592 1105 68-69 0.6376546891319279 1105 70-71 0.3896279204622246 1105 72-73 0.4554736490482867 1105 74-75 0.5195314886560567 1105 76-77 0.46794268790347715 1105 78-79 0.5732221441184393 1105 80-81 0.5091301238826169 1105 82-83 0.35627541518256933 1105 84-85 0.5226577802095065 1105 86-87 0.609693862530488 1105 88-89 0.5507601184148463 1105 90-91 0.5533267979811889 1105 92-93 0.5448864822198836 1105 94-95 0.5450531847996665 1105 96-97 0.45156427907848595 1105 98-99 0.46927762398368955 1105 100-101 0.5793974257836751 1106 1 0.2629604800719676 1106 2 0.2508667786897867 1106 3 0.26217674583425676 1106 4 0.23164940669285272 1106 5 0.21566480690906786 1106 6 0.23169998010335036 1106 7 0.31048539759459004 1106 8 0.3807694498723819 1106 9 0.3650305754672445 1106 10-11 0.42282934520783044 1106 12-13 0.5029300652646143 1106 14-15 0.4390722368753188 1106 16-17 0.48129554131361374 1106 18-19 0.5152554415228963 1106 20-21 0.48031175148224037 1106 22-23 0.4026102724834075 1106 24-25 0.3598392990213668 1106 26-27 0.34278256627068515 1106 28-29 0.28924601696811436 1106 30-31 0.4996367545949383 1106 32-33 0.44172758447061256 1106 34-35 0.5547182682839988 1106 36-37 0.3445554054946456 1106 38-39 0.21720620658161494 1106 40-41 0.44052534181295755 1106 42-43 0.43454580688482736 1106 44-45 0.47662027995949785 1106 46-47 0.6647572676173112 1106 48-49 0.7309088892465425 1106 50-51 0.838481564422473 1106 52-53 0.855247450874991 1106 54-55 0.9469698622881708 1106 56-57 0.8262880641981027 1106 58-59 0.836664433467476 1106 60-61 0.8675925124870361 1106 62-63 0.808249713950346 1106 64-65 0.713685090339041 1106 66-67 0.7138331417049173 1106 68-69 0.6673868416384465 1106 70-71 0.7577435702128881 1106 72-73 0.6994775242393416 1106 74-75 0.7633187352633328 1106 76-77 0.7859954236957272 1106 78-79 0.7468839680828694 1106 80-81 0.7506024841981898 1106 82-83 0.7113412432537736 1106 84-85 0.6606458458561661 1106 86-87 0.7480037350042856 1106 88-89 0.8326609691041931 1106 90-91 0.9340995396402647 1106 92-93 1.055946119248798 1106 94-95 1.2563770218563768 1106 96-97 1.1594818849514859 1106 98-99 1.0712185003017929 1106 100-101 1.584424752828184 1107 1 0.3191289276495368 1107 2 0.41759992979038074 1107 3 0.40281639148312465 1107 4 0.20270361003494486 1107 5 0.33212918651120305 1107 6 0.2459984930318484 1107 7 0.28957222844510966 1107 8 0.25888713385987927 1107 9 0.36070254780971567 1107 10-11 0.39947859513262074 1107 12-13 0.44868912131644123 1107 14-15 0.48242063274761193 1107 16-17 0.521541511920951 1107 18-19 0.6844904781656282 1107 20-21 0.7038771045908803 1107 22-23 0.6242688302289565 1107 24-25 0.5722025699683257 1107 26-27 0.6626337283314001 1107 28-29 0.6087582174066952 1107 30-31 0.7941132060264238 1107 32-33 0.579934008828765 1107 34-35 0.5529199188418161 1107 36-37 0.4387894612194998 1107 38-39 0.5960116229921582 1107 40-41 0.7719061036155779 1107 42-43 0.7155842283481775 1107 44-45 0.7332463802223756 1107 46-47 0.7552060702435526 1107 48-49 0.6522774222729666 1107 50-51 0.542686323400055 1107 52-53 0.5837991365278725 1107 54-55 0.621884958396322 1107 56-57 0.65209666750183 1107 58-59 0.7146115467351493 1107 60-61 0.7924193259862093 1107 62-63 0.8814834078254918 1107 64-65 0.8588080756427132 1107 66-67 0.8457486127416871 1107 68-69 0.6601896291890696 1107 70-71 0.6503668497919222 1107 72-73 0.7136948389657505 1107 74-75 0.6991699757974814 1107 76-77 0.7278515165036374 1107 78-79 0.6609773601840843 1107 80-81 0.8176247880909102 1107 82-83 0.8388687416516092 1107 84-85 0.7479862287193164 1107 86-87 0.6748163622511321 1107 88-89 0.6895984562977695 1107 90-91 0.6953822750496563 1107 92-93 0.7742412185830823 1107 94-95 0.6806534314353634 1107 96-97 0.7201881676214974 1107 98-99 0.8426962421852444 1107 100-101 0.9574923446094061 1108 1 -0.050406399564451476 1108 2 0.008399766772573258 1108 3 -0.02842693832021581 1108 4 -0.02385383264381602 1108 5 0.003442220834479315 1108 6 0.08786427503876126 1108 7 0.045572703913705936 1108 8 -0.01608114250470294 1108 9 -0.1104093900541514 1108 10-11 -0.22004000834817816 1108 12-13 -0.06996633170118827 1108 14-15 -0.027006921583939913 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-2.971869145000465 2316 78-79 -3.1649870151433532 2316 80-81 -3.0107891016287134 2316 82-83 -3.4962881396836174 2316 84-85 -3.2051341646877027 2316 86-87 -3.659512260294054 2316 88-89 -3.464349771695044 2316 90-91 -3.892291728229445 2316 92-93 -3.892960210561018 2316 94-95 -4.101469700210064 2316 96-97 -4.269331601356868 2316 98-99 -4.820179629289353 2316 100-101 -5.052293251959632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3091.0 3 1177.0 4 1055.0 5 1181.0 6 1367.0 7 1685.0 8 2141.0 9 2668.0 10 3480.0 11 4890.0 12 6694.0 13 8733.0 14 9947.0 15 12853.0 16 17164.0 17 23435.0 18 20463.0 19 11288.0 20 10636.0 21 10897.0 22 11937.0 23 13044.0 24 14920.0 25 16998.0 26 20073.0 27 23616.0 28 28580.0 29 34646.0 30 42562.0 31 52867.0 32 67902.0 33 88827.0 34 122061.0 35 176210.0 36 264553.0 37 399116.0 38 390680.0 39 71496.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.318051377657525 19.912038915083105 15.07257466437947 21.6973350428799 2 14.68165766748678 21.153947396789377 41.326266357340224 22.838128578383618 3 18.168891919399556 27.107037748241442 30.73385709916622 23.99021323319278 4 11.857199669613632 17.622612309048666 39.621740706880395 30.89844731445731 5 12.62359844828382 38.50707523977145 34.54409808002216 14.325228231922566 6 28.898048567035595 38.08139534883721 18.707973536626 14.312582547501194 7 26.84420936590484 34.20414484747842 22.063907547662836 16.887738238953904 8 25.71826473641093 34.31135432453842 20.881437969288285 19.08894296976237 9 26.816419809068254 16.82821760921994 21.566549223712776 34.788813357999025 10-11 23.67295140042762 27.513766012276864 28.325067246860247 20.48821534043527 12-13 25.353172400699535 25.38161329067367 28.65655418303042 20.608660125596376 14-15 22.631331329724695 26.036345835155707 27.773235774445563 23.55908706067403 16-17 20.60731195651683 29.616513212104312 28.13221081392019 21.643964017458664 18-19 20.516923058403748 27.72132940841897 31.353588874081574 20.408158659095708 20-21 21.705043007247284 26.77869448554305 31.15392331419114 20.362339193018535 22-23 21.401377875912946 26.789855997200025 31.090058348750176 20.718707778136856 24-25 21.076940853925848 27.069952178127267 31.282382656694395 20.57072431125249 26-27 20.994512526147837 27.023991594595635 31.259752960914973 20.72174291834156 28-29 20.593411892801267 26.96917091471634 31.602907906761303 20.83450928572109 30-31 20.771925761504022 26.900301172809428 31.520757263137213 20.80701580254934 32-33 20.710731327185908 27.096199478799697 31.310643048477843 20.88242614553655 34-35 21.079022114120775 26.97282079611637 31.084817082524157 20.863340007238698 36-37 21.272622683528695 26.625718894084294 31.0200807843324 21.081577638054608 38-39 20.84443054212528 27.102805143791254 31.132188497401586 20.92057581668188 40-41 20.900798744346915 27.01403661536125 31.24675252410182 20.83841211619002 42-43 20.88555769185943 27.103755943031118 30.731495677556303 21.27919068755315 44-45 20.93887427121174 27.201001944437902 30.50080679886572 21.35931698548464 46-47 21.32420815766844 27.400264822583154 29.895301592218836 21.380225427529567 48-49 21.305951220881507 27.619812406514043 29.566525911400376 21.50771046120408 50-51 21.249350847257116 27.6278655456793 29.493991702875523 21.628791904188063 52-53 21.35362634073935 27.971294962532113 29.22450229755096 21.450576399177578 54-55 21.293566632869066 27.754982848448464 29.261511968486765 21.689938550195702 56-57 21.31816337226921 28.104190170773602 28.976243191276414 21.601403265680773 58-59 21.56201288765026 27.62726423041669 29.15587118966762 21.65485169226543 60-61 21.463134529916758 27.759308477308657 29.43841351605242 21.339143476722164 62-63 21.36650181850995 27.74394477544974 29.602659962451717 21.286893443588593 64-65 21.38663592160008 27.7519424532558 29.368890671211588 21.49253095393253 66-67 21.293155984374213 27.982322594845332 28.906728850274266 21.81779257050619 68-69 21.160541518173595 27.912693722181448 29.394694895164914 21.53206986448004 70-71 21.310936862093282 27.78043319728 29.315547093878802 21.59308284674791 72-73 21.390559699790412 27.820041676838574 29.009014608950046 21.780384014420967 74-75 21.199465683125226 28.342833107693327 28.800070587179555 21.657630622001893 76-77 21.24150835270503 27.99607025903551 29.08687670575601 21.675544682503453 78-79 21.20613413295368 27.926194059354316 28.950939521315817 21.916732286376195 80-81 21.174666778363406 28.41544966548666 28.623953031186964 21.785930524962968 82-83 21.322984317279573 28.253691286945777 28.81105399263184 21.612270403142812 84-85 21.220785008796987 28.03405897529799 28.618452797678266 22.12670321822675 86-87 21.13900020458019 28.004746460963293 28.86024766237048 21.996005672086035 88-89 21.460061935030904 27.732626220834746 28.74968969797269 22.057622146161656 90-91 21.14435601295539 27.717634279779556 29.126659573982604 22.01135013328245 92-93 21.28789620685012 27.920899923097426 28.95992465704135 21.831279213011104 94-95 21.05248513740366 27.65050783618595 29.004102645601055 22.292904380809333 96-97 21.142223432155102 27.657536519835297 28.84173325893249 22.358506789077115 98-99 20.96531839009133 27.682957558434723 28.878216338529622 22.473507712944333 100-101 20.811618717685164 27.77577122940717 29.059560876921836 22.353049175985827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 595.0 1 445.0 2 371.5 3 450.0 4 398.5 5 421.0 6 760.5 7 1586.5 8 2821.0 9 3483.5 10 3094.0 11 2662.5 12 2757.5 13 2934.0 14 3015.5 15 3178.0 16 3433.0 17 3678.0 18 3845.0 19 4194.0 20 4734.0 21 5324.5 22 6158.0 23 7337.0 24 8612.0 25 10629.5 26 13821.5 27 16938.5 28 19160.5 29 22189.5 30 26780.0 31 31972.0 32 38553.5 33 46143.5 34 53854.0 35 59794.0 36 67493.5 37 74721.0 38 74471.0 39 72258.0 40 70558.5 41 69898.5 42 68880.0 43 69638.5 44 73035.0 45 74181.0 46 71317.5 47 69057.5 48 74555.5 49 71916.5 50 60777.5 51 55084.0 52 52173.0 53 50635.5 54 48605.0 55 45328.0 56 41528.5 57 37429.0 58 34349.5 59 31335.5 60 27132.0 61 22575.0 62 18198.5 63 14349.5 64 10948.5 65 8094.0 66 5754.0 67 4315.5 68 2942.5 69 1643.5 70 1111.0 71 815.0 72 593.5 73 459.0 74 332.0 75 235.5 76 160.0 77 80.0 78 41.5 79 22.5 80 16.5 81 13.5 82 8.5 83 9.5 84 9.5 85 8.0 86 8.0 87 6.0 88 3.5 89 3.0 90 2.5 91 1.5 92 2.5 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033384178205344776 2 0.02586521915008694 3 0.021403970110633965 4 0.046517293355195125 5 0.09067864620640946 6 0.10852364236422138 7 0.05554004422150453 8 0.08250804403302928 9 0.06842252740284628 10-11 0.06864809617450401 12-13 0.06759544190676792 14-15 0.06120432670979876 16-17 0.049625129764701695 18-19 0.060051419654659224 20-21 0.055489917827802805 22-23 0.03285785107147673 24-25 0.007945033401722444 26-27 0.002506319685085944 28-29 0.0060151672442062655 30-31 0.00446124903945298 32-33 0.006942505527688066 34-35 0.006165546425311422 36-37 0.006992631921389785 38-39 0.004310869858347824 40-41 0.006942505527688066 42-43 0.003934921905584933 44-45 0.0018045501732618797 46-47 0.0018296133701127392 48-49 2.506319685085944E-4 50-51 0.0024561932913842255 52-53 0.005789598472548531 54-55 0.012005271291561672 56-57 0.009097940456861978 58-59 0.005087828960724467 60-61 0.002756951653594539 62-63 0.009624267590730026 64-65 0.0028572044409979763 66-67 0.021729791669695138 68-69 0.04493831195359098 70-71 0.05669295127664406 72-73 0.0042106170709443865 74-75 0.01305792555929777 76-77 0.021228527732677947 78-79 0.025589523984727493 80-81 0.07338504037931645 82-83 0.028296349244620312 84-85 0.012055397685263392 86-87 0.031529501638381176 88-89 0.046717798930002 90-91 0.04428666883546863 92-93 0.04388565768585488 94-95 0.013458936708911521 96-97 0.01784499615781192 98-99 0.03731910011092971 100-101 0.07438756825335081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.44711799613895 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62265382830817 34.66007207548459 2 13.668472669311251 12.69722325797 3 4.771922646294232 6.649261626626275 4 2.168842789878071 4.029459879061678 5 1.1713590517402916 2.7203126046013377 6 0.7227103854048579 2.014068872876069 7 0.45579744410576306 1.4819334368103245 8 0.35028108504960026 1.3015637511291491 9 0.25416006086795095 1.0624502103335618 >10 1.5129452451071135 13.507373927204444 >50 0.177218272073047 5.7098474100639125 >100 0.1155601955442808 9.914699245359342 >500 0.006784114104841618 2.1406966775220866 >1k 9.69159157834517E-4 0.6931255737640438 >5k 2.1536870174100375E-4 0.7497940161853645 >10k+ 1.0768435087050188E-4 0.6681174350079447 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13358 0.6695883670675609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8967 0.44948337232331326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6024 0.30196139565915453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2193 0.10992718138786951 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2057 0.10310999184443574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.0126393701718884E-5 0.0 6 0.0 0.0 0.0 5.0126393701718884E-5 0.0 7 0.0 0.0 0.0 5.0126393701718884E-5 0.0 8 0.0 0.0 0.0 5.0126393701718884E-5 0.0 9 0.0 0.0 0.0 5.0126393701718884E-5 0.0 10-11 0.0 0.0 0.0 1.5037918110515666E-4 0.0 12-13 0.0 0.0 0.0 2.0050557480687554E-4 0.0 14-15 0.0 0.0 0.0 2.25568771657735E-4 0.0 16-17 0.0 0.0 0.0 3.007583622103133E-4 0.0 18-19 0.0 0.0 0.0 3.007583622103133E-4 0.0 20-21 0.0 0.0 0.0 4.010111496137511E-4 0.0 22-23 0.0 0.0 0.0 4.7620074016632935E-4 0.0 24-25 0.0 0.0 0.0 9.0227508663094E-4 0.0 26-27 0.0 0.0 0.0 0.0012030334488412533 0.0 28-29 0.0 0.0 0.0 0.0030326468189539922 0.0 30-31 0.0 0.0 0.0 0.007844780614319005 0.0 32-33 0.0 0.0 0.0 0.01258172481913144 0.0 34-35 0.0 0.0 0.0 0.0169928474648827 5.0126393701718884E-5 36-37 0.0 0.0 0.0 0.02020093666179271 5.0126393701718884E-5 38-39 0.0 0.0 0.0 0.02250675077207178 5.0126393701718884E-5 40-41 0.0 0.0 0.0 0.026391546283954993 5.0126393701718884E-5 42-43 0.0 0.0 0.0 0.030827732126557112 5.0126393701718884E-5 44-45 0.0 0.0 0.0 0.035715055512474704 5.0126393701718884E-5 46-47 0.0 0.0 0.0 0.04170515955983011 5.0126393701718884E-5 48-49 0.0 0.0 0.0 0.04787070598514154 5.0126393701718884E-5 50-51 0.0 0.0 0.0 0.05864788063101109 5.0126393701718884E-5 52-53 0.0 0.0 0.0 0.0822574120645207 5.0126393701718884E-5 54-55 0.0 0.0 0.0 0.10980186540361522 5.0126393701718884E-5 56-57 0.0 0.0 0.0 0.14286022204989882 5.0126393701718884E-5 58-59 0.0 0.0 0.0 0.18757296523183206 5.0126393701718884E-5 60-61 0.0 0.0 0.0 0.2548676487763897 5.0126393701718884E-5 62-63 0.0 0.0 0.0 0.29880343285594624 1.0025278740343777E-4 64-65 0.0 0.0 0.0 0.3393807485574877 1.0025278740343777E-4 66-67 0.0 0.0 0.0 0.42880623492135417 1.0025278740343777E-4 68-69 0.0 0.0 0.0 0.5500870444826631 1.0025278740343777E-4 70-71 0.0 0.0 0.0 0.6383345605945392 1.0025278740343777E-4 72-73 0.0 0.0 0.0 0.717885147399167 1.0025278740343777E-4 74-75 0.0 0.0 0.0 0.891021711244904 1.0025278740343777E-4 76-77 0.0 0.0 0.0 1.005685836837586 1.253159842542972E-4 78-79 0.0 2.5063196850859442E-5 0.0 1.0860635091382922 1.5037918110515666E-4 80-81 0.0 5.0126393701718884E-5 0.0 1.1983215678332917 1.5037918110515666E-4 82-83 0.0 5.0126393701718884E-5 0.0 1.4119101313963158 1.5037918110515666E-4 84-85 0.0 5.0126393701718884E-5 0.0 1.627453624313707 1.5037918110515666E-4 86-87 0.0 5.0126393701718884E-5 0.0 1.9371595477997772 1.5037918110515666E-4 88-89 0.0 5.0126393701718884E-5 0.0 2.3297494632716393 1.5037918110515666E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14160 0.0 33.703037 1 GGTATCA 9165 0.0 30.65163 1 GTATAGG 835 0.0 27.893831 1 ATCAACG 17330 0.0 27.375574 3 TCAACGC 17410 0.0 27.277086 4 CAACGCA 17550 0.0 27.003963 5 TATCAAC 17745 0.0 26.922869 2 AACGCAG 17940 0.0 26.4646 6 GTACATG 12020 0.0 25.743942 1 TACATGG 12585 0.0 24.174526 2 ACGCAGA 19795 0.0 23.886755 7 GAGTACT 9210 0.0 23.83662 12-13 ACATGGG 12395 0.0 23.663004 3 CGCAGAG 19885 0.0 23.587458 8 CGCGGAA 270 0.0 22.870937 82-83 GCAGAGT 20380 0.0 22.430487 9 AGTACTT 9770 0.0 22.129885 12-13 GTACTTT 9855 0.0 22.108608 14-15 CATGGGG 4170 0.0 21.317526 4 CATGGGA 8110 0.0 20.222252 4 >>END_MODULE