FastQCFastQC Report
Thu 26 May 2016
SRR937040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994957
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135230.6778592220283445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101520.5088831488598501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69260.347175402778105No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29960.15017867553034978No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.12215802145108892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA166400.030.2632521
GGTATCA112250.028.7796571
CGATAGC700.00839571427.1410754
GTACATG115200.025.5270841
TCAACGC202700.024.6037124
ATCAACG203300.024.53113
TATCAAC204950.024.4275282
CAACGCA204800.024.374625
AACGCAG208600.023.9575676
TACATGG121250.023.935712
ACATGGG118950.023.7184933
GAGTACT113350.022.79829212-13
GTATAGG11050.022.7864481
CATATAG6100.022.5804843
AGTACTT119850.021.99783112-13
CATGGGG39100.021.6226224
ACGCAGA230450.021.5628997
CGCAGAG231050.021.4457598
GTACTTT121050.021.38465514-15
GCAGAGT240500.020.3462879
Produced by FastQC (version 0.11.5)