##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994957 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1541592124542 33.0 31.0 34.0 31.0 34.0 2 32.28044313737088 33.0 31.0 34.0 31.0 34.0 3 32.40070537861217 34.0 31.0 34.0 31.0 34.0 4 36.06770772502866 37.0 35.0 37.0 35.0 37.0 5 35.919778220783705 37.0 35.0 37.0 35.0 37.0 6 36.01708407750142 37.0 35.0 37.0 35.0 37.0 7 35.97339942665431 37.0 35.0 37.0 35.0 37.0 8 35.92492469762506 37.0 35.0 37.0 35.0 37.0 9 37.61425384106023 39.0 37.0 39.0 35.0 39.0 10-11 37.535364170756566 39.0 37.0 39.0 35.0 39.0 12-13 37.43865857760343 39.0 37.0 39.0 34.5 39.0 14-15 38.61345708203235 40.0 38.0 41.0 34.0 41.0 16-17 38.624071596530655 40.0 38.0 41.0 34.5 41.0 18-19 38.780836629561435 40.0 38.0 41.0 35.0 41.0 20-21 38.79955407560163 40.0 38.0 41.0 35.0 41.0 22-23 38.700910094804044 40.0 38.0 41.0 34.0 41.0 24-25 38.60864269254926 40.0 38.0 41.0 34.0 41.0 26-27 38.40396209041097 40.0 38.0 41.0 34.0 41.0 28-29 38.250822699436625 40.0 38.0 41.0 34.0 41.0 30-31 38.196721783978305 40.0 37.5 41.0 34.0 41.0 32-33 38.033737819912915 40.0 37.0 41.0 33.5 41.0 34-35 37.89690504607367 40.0 37.0 41.0 33.0 41.0 36-37 37.73245638878432 40.0 37.0 41.0 33.0 41.0 38-39 37.55579719262119 40.0 37.0 41.0 33.0 41.0 40-41 37.216505669044494 40.0 36.0 41.0 32.0 41.0 42-43 36.98668066529755 40.0 36.0 41.0 31.0 41.0 44-45 36.70404875894568 39.0 36.0 41.0 31.0 41.0 46-47 36.24665794801592 39.0 35.5 41.0 30.0 41.0 48-49 35.814568434307105 39.0 35.0 40.0 28.5 41.0 50-51 35.52047437613943 39.0 35.0 40.0 27.5 41.0 52-53 35.26633857271109 39.0 35.0 40.0 27.0 41.0 54-55 34.882233551901116 38.0 34.5 40.0 26.0 41.0 56-57 34.69932184001961 38.0 34.0 40.0 26.0 41.0 58-59 34.391175599273566 38.0 34.0 40.0 25.0 41.0 60-61 34.03137360855396 37.5 33.5 40.0 24.0 41.0 62-63 33.67093776958601 37.0 33.0 39.5 23.0 41.0 64-65 33.352651961921985 36.5 33.0 39.0 22.5 40.0 66-67 33.033853862514334 36.0 32.5 39.0 22.0 40.0 68-69 32.59228619965242 35.5 32.0 39.0 20.5 40.0 70-71 32.16112327233118 35.0 31.5 38.0 19.5 40.0 72-73 31.759267492983557 35.0 31.0 37.5 18.5 39.5 74-75 31.276348312269388 35.0 31.0 37.0 17.5 39.0 76-77 29.820126448840753 33.5 29.5 35.5 13.0 37.5 78-79 30.486506225447464 34.0 30.0 36.0 12.5 38.0 80-81 30.3529534721801 34.0 30.0 36.0 9.5 37.0 82-83 30.011309266315013 34.0 30.0 35.0 7.0 37.0 84-85 29.56662449366077 34.0 29.5 35.0 4.0 36.5 86-87 29.20037424365538 34.0 29.0 35.0 2.0 36.0 88-89 28.82169966570708 34.0 29.0 35.0 2.0 35.5 90-91 28.381823518000637 33.5 29.0 35.0 2.0 35.0 92-93 27.918896497518492 33.0 27.5 35.0 2.0 35.0 94-95 27.38398296304131 33.0 27.0 34.5 2.0 35.0 96-97 26.635074089316213 32.5 25.5 34.0 2.0 35.0 98-99 25.81627649117249 32.0 24.5 34.0 2.0 35.0 100-101 22.644396596016854 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16084680299777432 1101 2 0.2252940661134346 1101 3 0.12176947952590922 1101 4 0.07178056282448608 1101 5 -0.0613821658839413 1101 6 0.030082004124899697 1101 7 0.07531619124890199 1101 8 0.052083792008765784 1101 9 0.0666890243301026 1101 10-11 0.1425326803979985 1101 12-13 0.19500667303211117 1101 14-15 0.2814928140529318 1101 16-17 0.21619773251637042 1101 18-19 0.10979183597128639 1101 20-21 -0.03810341052253108 1101 22-23 0.011722548201511529 1101 24-25 0.050184591481681196 1101 26-27 0.07414542813710767 1101 28-29 0.08075317240573554 1101 30-31 0.19937262630729435 1101 32-33 0.21432151450830617 1101 34-35 -0.0739797401850808 1101 36-37 -0.026276151508042744 1101 38-39 -0.04281888630210773 1101 40-41 0.1651550681247258 1101 42-43 0.033170902246233425 1101 44-45 -0.06017456876368499 1101 46-47 -0.08315687569007224 1101 48-49 -0.18740796795027137 1101 50-51 -0.1922474792263884 1101 52-53 -0.20108511247526906 1101 54-55 -0.45239097118017213 1101 56-57 -0.3128045654816276 1101 58-59 -0.12323348194178863 1101 60-61 -0.28712366912015597 1101 62-63 -0.21784165139524703 1101 64-65 -0.25463901828562996 1101 66-67 -0.286955739141284 1101 68-69 -0.6059785175319661 1101 70-71 -0.5051503046177146 1101 72-73 -0.403430020019357 1101 74-75 -0.3946228031867989 1101 76-77 -0.2414581562835778 1101 78-79 -0.3599933156441786 1101 80-81 -0.3109095284132195 1101 82-83 -0.2033305431805168 1101 84-85 -0.41402169909107656 1101 86-87 -0.49293555374745424 1101 88-89 -0.4094527281089597 1101 90-91 -0.391540207171591 1101 92-93 -0.5088953833085803 1101 94-95 -0.6332887681608668 1101 96-97 -0.5866087843321353 1101 98-99 -0.31312171449082626 1101 100-101 -0.11426625490419795 1102 1 -0.13441036657910388 1102 2 -0.06217492048863704 1102 3 0.0202050730317751 1102 4 0.0068334388858275474 1102 5 -0.032240986196647725 1102 6 0.044974732710898024 1102 7 0.0654158697049354 1102 8 0.13575064929386826 1102 9 0.14460724009956039 1102 10-11 0.19831280107013782 1102 12-13 0.21363701572020943 1102 14-15 0.1061094794455002 1102 16-17 0.14295232157212467 1102 18-19 0.11476465054025198 1102 20-21 0.12725137680204313 1102 22-23 -0.11799449275427065 1102 24-25 -0.08495204240199428 1102 26-27 -0.02495198861281267 1102 28-29 -0.04567285787408082 1102 30-31 -0.2071822979546738 1102 32-33 -0.15039735711918922 1102 34-35 0.0913037620254542 1102 36-37 0.030999207231019454 1102 38-39 0.3126442911317042 1102 40-41 0.34945761369427686 1102 42-43 0.27466964946501804 1102 44-45 0.2031212420681925 1102 46-47 0.008144223710068843 1102 48-49 0.14414950595718778 1102 50-51 0.0860859920123076 1102 52-53 0.2234663729578088 1102 54-55 0.476744015886176 1102 56-57 0.38284552064652644 1102 58-59 0.1141028268817692 1102 60-61 0.17923089411551274 1102 62-63 0.17872373559720245 1102 64-65 0.1179032852748989 1102 66-67 0.11582999564904384 1102 68-69 0.2561737401244457 1102 70-71 0.17238321644182975 1102 72-73 -0.1331345357870113 1102 74-75 -0.235899543195206 1102 76-77 0.07447187129807631 1102 78-79 -0.20447097593903507 1102 80-81 -0.022631040301387628 1102 82-83 0.14420546717270355 1102 84-85 0.039780561425491356 1102 86-87 -0.09765023748382973 1102 88-89 -0.10072914449169801 1102 90-91 -0.16874212562456847 1102 92-93 -0.033725869056713265 1102 94-95 -0.010124884645815513 1102 96-97 0.3199252354608113 1102 98-99 0.1921086886190615 1102 100-101 0.2645357563331352 1103 1 0.16145152287603537 1103 2 0.2115802163909848 1103 3 0.20401155901585355 1103 4 0.16765885551102144 1103 5 0.025153864858012298 1103 6 0.09451415895572524 1103 7 0.09955163871344297 1103 8 0.15427581794534717 1103 9 0.16082023111508192 1103 10-11 0.1542475216145789 1103 12-13 0.2371277451696585 1103 14-15 0.2219282581373747 1103 16-17 0.25841369374106904 1103 18-19 0.27716747141128906 1103 20-21 0.17821228388601895 1103 22-23 0.1499785888865759 1103 24-25 0.28286187563277565 1103 26-27 0.16561085013123744 1103 28-29 0.10233014101100224 1103 30-31 0.21572136465213276 1103 32-33 0.22834247742655833 1103 34-35 0.1935690194261852 1103 36-37 0.2101573497267566 1103 38-39 0.21505118641920262 1103 40-41 0.34473882719727555 1103 42-43 0.10572654879828747 1103 44-45 0.2529365328017903 1103 46-47 0.06307741831751201 1103 48-49 0.10786741813739553 1103 50-51 0.07320324569807468 1103 52-53 0.08351947642436386 1103 54-55 0.30643063311085683 1103 56-57 0.08069871849059496 1103 58-59 0.09288856126847378 1103 60-61 -0.01908497197725012 1103 62-63 0.28875437248792934 1103 64-65 0.21295838298571113 1103 66-67 0.34483833710375933 1103 68-69 0.2667290923967798 1103 70-71 0.1624670427270445 1103 72-73 0.23401567842417847 1103 74-75 0.14109246335823045 1103 76-77 0.2417639919208554 1103 78-79 0.24695055860507154 1103 80-81 0.14983385276487127 1103 82-83 0.30924833447346955 1103 84-85 0.0600666262767362 1103 86-87 -0.09860000973898408 1103 88-89 0.26135158981983153 1103 90-91 0.10537176040204344 1103 92-93 -0.03948769542642694 1103 94-95 0.13964402290216427 1103 96-97 0.18858294795881392 1103 98-99 -0.09618017461874828 1103 100-101 0.4210178736548471 1104 1 -0.030429513731611735 1104 2 0.07396068642133713 1104 3 -0.05910813732043607 1104 4 0.030121151556770087 1104 5 -0.08229571519225942 1104 6 -0.026896451745571426 1104 7 0.0631970381135929 1104 8 0.058468606242442434 1104 9 -0.11721700709014726 1104 10-11 0.12207283431793314 1104 12-13 0.09297725820790959 1104 14-15 0.13548867094380768 1104 16-17 0.09511960328570268 1104 18-19 0.11371486728865676 1104 20-21 0.04848266263577017 1104 22-23 0.10687877123061895 1104 24-25 0.10067809440219122 1104 26-27 0.12000981284106871 1104 28-29 0.02499873944382358 1104 30-31 0.05949068199048213 1104 32-33 0.036875709259440725 1104 34-35 0.1267849330737576 1104 36-37 0.04042750742991785 1104 38-39 -0.009866522204177386 1104 40-41 0.22072675029788513 1104 42-43 0.11711064166578211 1104 44-45 0.06634335701392047 1104 46-47 -0.018339273103784137 1104 48-49 -0.05132693687581735 1104 50-51 0.014561138728588219 1104 52-53 -0.007083639479283477 1104 54-55 -0.06092968677845079 1104 56-57 -0.03844674644792434 1104 58-59 -0.16631463560801052 1104 60-61 -0.2115489704694795 1104 62-63 -0.07622504303387956 1104 64-65 -0.031362271063976266 1104 66-67 -0.12961100483668275 1104 68-69 -0.06634641789119655 1104 70-71 0.006874583595454453 1104 72-73 0.03552922702844796 1104 74-75 -0.23188990488055694 1104 76-77 0.05316908421361788 1104 78-79 0.06440195356412559 1104 80-81 0.08152813215689036 1104 82-83 0.1651453531367828 1104 84-85 0.17140846822300304 1104 86-87 0.058471134685095905 1104 88-89 0.10374237147932419 1104 90-91 0.14946531817392383 1104 92-93 0.05181927933005781 1104 94-95 0.10587052894682003 1104 96-97 0.2070876492705871 1104 98-99 0.23957455597200195 1104 100-101 0.7609962421139009 1105 1 -0.0029160492801665328 1105 2 0.12394681837982802 1105 3 0.08316508799960332 1105 4 0.004792010980338546 1105 5 -0.11391356177308154 1105 6 0.0029546149343815387 1105 7 0.12926301392567296 1105 8 0.10729849560724602 1105 9 0.2340830251970587 1105 10-11 0.06816790725610389 1105 12-13 0.15789235831542214 1105 14-15 0.15309185260742453 1105 16-17 -0.03954895481580678 1105 18-19 0.02785719976443346 1105 20-21 0.11584871367015381 1105 22-23 0.18284300799887632 1105 24-25 0.0915645799742748 1105 26-27 0.02135909170291228 1105 28-29 -0.01669726621673817 1105 30-31 -0.022197020019220304 1105 32-33 0.1353352618068655 1105 34-35 -0.044326626947821524 1105 36-37 0.034413049459956824 1105 38-39 0.10735503070733898 1105 40-41 0.1421254388877955 1105 42-43 0.15071048040149293 1105 44-45 0.08818240959053725 1105 46-47 -0.04075322684377625 1105 48-49 -0.17135129396543647 1105 50-51 -0.22824594178676705 1105 52-53 0.03780791930575589 1105 54-55 0.2633112418908681 1105 56-57 0.07264936122646759 1105 58-59 -0.07000860099201134 1105 60-61 -0.06638063047834208 1105 62-63 -0.050758724771348795 1105 64-65 0.04552872011885967 1105 66-67 -0.015745582372339584 1105 68-69 0.055641898605848894 1105 70-71 -0.034135855647924984 1105 72-73 0.019025076920421213 1105 74-75 -0.08175043016590067 1105 76-77 -0.1944323456973862 1105 78-79 -0.11307248286957972 1105 80-81 -0.135834956354703 1105 82-83 -0.008367782431694337 1105 84-85 -0.36794392429323963 1105 86-87 -0.28522079557454205 1105 88-89 -0.07652435002979274 1105 90-91 -0.14996416004423452 1105 92-93 0.03713278723183677 1105 94-95 -0.03307608381624405 1105 96-97 0.14400072627557492 1105 98-99 0.3079551637271152 1105 100-101 0.5534381380254523 1106 1 -0.009447714685975939 1106 2 0.08755418124283665 1106 3 0.10724608103660671 1106 4 0.10360552320031502 1106 5 0.040586523141463715 1106 6 0.11384529131732535 1106 7 0.14598731955627642 1106 8 0.15484581616737358 1106 9 0.21020177432861686 1106 10-11 0.10269580168417036 1106 12-13 0.21800317179676654 1106 14-15 0.34682149241536564 1106 16-17 0.23148476397809503 1106 18-19 0.2923896486999169 1106 20-21 0.30435707389309385 1106 22-23 0.2089137032174122 1106 24-25 0.2102032261276463 1106 26-27 0.2962197546539187 1106 28-29 0.26900929999344214 1106 30-31 0.18012828760301858 1106 32-33 0.059701008051327165 1106 34-35 -0.023110052400568293 1106 36-37 -0.00805435493398221 1106 38-39 0.10884151771541184 1106 40-41 0.20951284992050745 1106 42-43 0.3254168497643235 1106 44-45 0.0767290778525691 1106 46-47 0.11646943067270144 1106 48-49 -0.025590170160029402 1106 50-51 0.20127112603679365 1106 52-53 0.1273884221542474 1106 54-55 0.30051973411733 1106 56-57 0.323096127526199 1106 58-59 0.3466188248621549 1106 60-61 0.15615723368926382 1106 62-63 0.08732100998648917 1106 64-65 -0.0017657866820215418 1106 66-67 0.18181100159269903 1106 68-69 0.04400185136248069 1106 70-71 -0.061098636058908085 1106 72-73 0.16325484410017665 1106 74-75 -0.10379106177572694 1106 76-77 0.17095783663729947 1106 78-79 0.013488786834454203 1106 80-81 -0.021075843480115708 1106 82-83 0.06998373676671221 1106 84-85 0.2409560849701684 1106 86-87 0.19367311528062459 1106 88-89 0.32624946078059835 1106 90-91 0.25564560358455424 1106 92-93 0.45030332265664086 1106 94-95 0.4490262459300922 1106 96-97 0.7294855497305157 1106 98-99 0.5863687284397194 1106 100-101 1.0029067466620312 1107 1 0.3017059755270779 1107 2 0.33502886266251153 1107 3 0.2703967020666127 1107 4 0.145565920275871 1107 5 0.07406634021118208 1107 6 0.13850447685031497 1107 7 0.1969242051202187 1107 8 0.17181787526658354 1107 9 0.22292754892676925 1107 10-11 0.2698709107840287 1107 12-13 0.2228587152906627 1107 14-15 0.40502043999946835 1107 16-17 0.3075009948992431 1107 18-19 0.3594550039883657 1107 20-21 0.3235754349319109 1107 22-23 0.25775959883903 1107 24-25 0.2769159959345515 1107 26-27 0.24042187605231646 1107 28-29 0.2735945461166409 1107 30-31 0.21678518868725405 1107 32-33 0.18369611218121662 1107 34-35 0.15139905269715115 1107 36-37 0.10439044118768948 1107 38-39 0.02077961525788652 1107 40-41 0.2948679639903631 1107 42-43 0.2628124910012346 1107 44-45 0.13845987475025368 1107 46-47 0.024662601740423895 1107 48-49 -0.0213689535602839 1107 50-51 -0.13589923106091106 1107 52-53 0.07606189421117193 1107 54-55 0.12984710782960462 1107 56-57 0.022626812817229336 1107 58-59 -0.048641488944376476 1107 60-61 -0.010598260540760407 1107 62-63 0.1777354804870228 1107 64-65 0.15368026937417056 1107 66-67 0.09227227277507666 1107 68-69 -0.1637690890543908 1107 70-71 -0.10583830045420939 1107 72-73 0.17268200985785853 1107 74-75 0.0955963005143019 1107 76-77 -0.16470735647273216 1107 78-79 0.2393870896285435 1107 80-81 0.3104329826009682 1107 82-83 0.42752339854622434 1107 84-85 0.4027197971121872 1107 86-87 0.49404324829308877 1107 88-89 0.4415672563747499 1107 90-91 0.3347468212306097 1107 92-93 0.39403569338255906 1107 94-95 0.6539639685439553 1107 96-97 0.685726258258395 1107 98-99 0.9471486889414251 1107 100-101 1.5742031277290103 1108 1 0.010058476831524388 1108 2 0.05848649707244391 1108 3 0.08137194785403778 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-1.162640574613171 2316 72-73 -1.3190759667727292 2316 74-75 -0.9764330501774694 2316 76-77 -0.8568718798474464 2316 78-79 -0.8971239577105301 2316 80-81 -1.1497782499032603 2316 82-83 -0.9900160683562973 2316 84-85 -0.8101037381635798 2316 86-87 -0.8173038441049592 2316 88-89 -1.346468221947351 2316 90-91 -1.263955431930242 2316 92-93 -0.9677126962320735 2316 94-95 -1.1642475439350726 2316 96-97 -1.1428538665000723 2316 98-99 -1.4100045081799273 2316 100-101 -2.4930926338342694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 15.0 5 37.0 6 90.0 7 235.0 8 511.0 9 1150.0 10 1904.0 11 3438.0 12 5363.0 13 7721.0 14 9669.0 15 13584.0 16 18619.0 17 26374.0 18 23042.0 19 12474.0 20 10244.0 21 10199.0 22 10868.0 23 11908.0 24 13593.0 25 16278.0 26 19679.0 27 24211.0 28 30571.0 29 37885.0 30 47882.0 31 59905.0 32 76997.0 33 100849.0 34 138866.0 35 198398.0 36 303383.0 37 449489.0 38 294843.0 39 14677.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37434213593927 20.833001173714216 15.470114909680161 21.322541780666356 2 15.023870966448309 22.054527262628753 40.3058912462429 22.615710524680033 3 18.44422165969072 27.44996115190857 30.701771974234944 23.40404521416577 4 12.33039108111102 18.365107618860957 38.85066194409203 30.45383935593599 5 12.591000391496554 39.113162555284475 34.17885226844934 14.116984784769631 6 29.09711838400527 38.79126216755549 17.932867725971036 14.178751722468203 7 26.929242478773947 34.603855527495874 21.619646367785926 16.847255625944257 8 26.18061441925816 34.71453269418839 20.228155293572744 18.8766975929807 9 27.066160857683073 16.88037229893792 21.206432623075397 34.847034220303605 10-11 23.951944828886035 27.957820644755753 27.742427531019466 20.347806995338747 12-13 25.659639882204882 25.74736711767656 28.271304747121228 20.321688252997326 14-15 22.672899904105325 26.458408629618475 27.52974213706272 23.338949329213488 16-17 20.623000896761184 29.99423045208493 27.885262689872516 21.49750596128137 18-19 20.45228804496163 27.906451579754936 31.432913831480608 20.208346543802826 20-21 21.67210209039078 26.89299116062755 31.37588737093584 20.059019378045832 22-23 21.381151051945363 27.01327319400298 31.202607819950945 20.402967934100715 24-25 21.04193348688811 27.236640609139602 31.542523728724355 20.17890217524794 26-27 21.095855116422513 27.22487015361511 31.468603152905565 20.210671577056807 28-29 20.524558460997095 27.17022547888322 31.807826145364754 20.497389914754926 30-31 20.84291060983034 27.176081282975346 31.584924282810178 20.39608382438414 32-33 20.838786678182394 27.404430079305126 31.348733529060585 20.408049713451895 34-35 21.068649599966317 27.10133601877133 31.3989725091819 20.431041872080453 36-37 21.35851859946474 26.693611733385197 31.27449029361033 20.67337937353973 38-39 20.97820259309009 27.26251208453573 31.297750678450964 20.46153464392322 40-41 20.998199196217435 27.25918165561163 31.322531852421815 20.420087295749127 42-43 21.006568061366735 27.47249690093571 30.665773748506858 20.855161289190693 44-45 21.06790841014027 27.453699039117524 30.458405453405295 21.01998709733691 46-47 21.537736246285625 27.78961599076068 29.798285873544827 20.874361889408867 48-49 21.539338365862477 28.075201715126834 29.34588232463447 21.039577594376212 50-51 21.533412935682506 28.030152648031297 29.184361647307455 21.252072768978746 52-53 21.627799379533634 28.412552488757868 28.970435865342054 20.98921226636644 54-55 21.530614444321355 28.198652903295663 28.962554080113005 21.308178572269977 56-57 21.551735805304688 28.530672151173487 28.62824337214962 21.289348671372206 58-59 21.71185400766133 28.106888680139992 28.881367690683597 21.299889621515085 60-61 21.605576156467233 28.27034634177518 29.1083989478288 21.015678553928787 62-63 21.64693816632092 28.16780716772521 29.26166560524001 20.923589060713855 64-65 21.553588538691713 28.11897307985065 29.123281195996604 21.20415718546103 66-67 21.523922570762178 28.476729072355944 28.65111879604423 21.34822956083765 68-69 21.468319938284274 28.244016715176567 29.02162882493251 21.266034521606645 70-71 21.556022067765245 28.224523370557396 29.04767848352787 21.171776078149485 72-73 21.645997377877173 28.21195634874785 28.710164698487205 21.431881574887772 74-75 21.587367947211217 28.72336292639181 28.3289559066687 21.360313219728273 76-77 21.574059778762038 28.485590659643623 28.589878674015868 21.350470887578474 78-79 21.500653399507055 28.400998320259497 28.446688665536147 21.6516596146973 80-81 21.513856250300766 28.86001989060169 28.18452643525128 21.441597423846265 82-83 21.660257584692992 28.816682878390022 28.21368697196504 21.30937256495195 84-85 21.428548155013782 28.661894680276994 28.110440263741683 21.799116900967537 86-87 21.365850479774533 28.538695880468033 28.258337625426233 21.837116014331205 88-89 21.59050379655146 28.386725667043876 28.18506167594657 21.837708860458093 90-91 21.417453915491432 28.386999591963995 28.45444583798264 21.741100654561933 92-93 21.383373522179554 28.55936959026425 28.27026554213087 21.786991345425328 94-95 21.17993019398413 28.297702657250255 28.296725192573074 22.22564195619254 96-97 21.497389786167734 28.308432560891834 28.07409102426801 22.120086628672425 98-99 21.14928510542524 28.43229473301137 28.011681527982358 22.406738633581032 100-101 20.797892244720774 28.846190959680808 27.99076754263395 22.36514925296447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 980.0 1 701.0 2 453.5 3 502.0 4 473.5 5 469.0 6 773.5 7 1616.0 8 2736.0 9 3309.5 10 3142.0 11 2876.5 12 2948.5 13 3126.0 14 3344.0 15 3640.5 16 3850.5 17 4063.0 18 4394.5 19 4802.0 20 5342.5 21 5903.5 22 6694.0 23 7755.0 24 9031.0 25 11174.0 26 14327.5 27 17532.5 28 19692.0 29 22628.0 30 27434.5 31 32427.5 32 38740.5 33 45813.5 34 53546.0 35 59461.0 36 66451.5 37 73270.5 38 73974.0 39 73175.0 40 71349.0 41 69333.0 42 67986.0 43 69263.5 44 72525.0 45 73649.0 46 70761.0 47 69144.5 48 74870.0 49 71499.5 50 60741.5 51 54897.0 52 51190.0 53 49754.0 54 47602.0 55 44788.5 56 41099.0 57 36923.5 58 34470.5 59 31515.0 60 27074.0 61 22385.0 62 18191.0 63 14360.0 64 10678.5 65 7969.0 66 5738.0 67 4368.5 68 2981.5 69 1662.5 70 1156.5 71 871.0 72 658.5 73 480.5 74 330.5 75 253.0 76 171.0 77 75.5 78 44.5 79 24.5 80 9.5 81 8.5 82 9.0 83 4.5 84 0.0 85 0.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021754854866545995 2 0.0032582155906117276 3 3.5088475591203217E-4 4 0.0 5 0.0024060668976825066 6 0.0 7 1.5037918110515666E-4 8 0.0 9 5.0126393701718884E-5 10-11 0.0 12-13 0.006892379133986346 14-15 0.0030075836221031328 16-17 0.0 18-19 5.764535275697672E-4 20-21 9.524014803326588E-4 22-23 0.0017042973858584422 24-25 6.015167244206266E-4 26-27 1.7544237795601609E-4 28-29 1.5037918110515666E-4 30-31 2.5063196850859442E-5 32-33 2.0050557480687554E-4 34-35 0.0 36-37 0.0017042973858584422 38-39 0.007794654220617286 40-41 7.268327086749238E-4 42-43 0.0 44-45 0.0010025278740343777 46-47 2.506319685085944E-4 48-49 1.253159842542972E-4 50-51 0.0020551821417704743 52-53 3.007583622103133E-4 54-55 0.0 56-57 2.0050557480687554E-4 58-59 1.5037918110515666E-4 60-61 0.0017293605827093017 62-63 2.5063196850859442E-5 64-65 7.518959055257833E-5 66-67 0.0 68-69 1.7544237795601609E-4 70-71 5.513903307189077E-4 72-73 4.2607434646461055E-4 74-75 0.003183026000059149 76-77 5.0126393701718884E-5 78-79 3.007583622103133E-4 80-81 0.0022556877165773497 82-83 7.518959055257833E-5 84-85 0.0025313828819368035 86-87 4.762007401663294E-4 88-89 0.002731888456743679 90-91 0.001754423779560161 92-93 2.5063196850859442E-5 94-95 0.0 96-97 2.7569516535945384E-4 98-99 6.26579921271486E-4 100-101 0.001929866157516177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.946120896585725 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68506461722025 33.11857822558313 2 14.02651161334694 12.608745734617106 3 4.903917969770758 6.61236269808767 4 2.2460795625262446 4.0381025424261985 5 1.2906882380107263 2.9005714792715658 6 0.7779761726584826 2.0980206666582726 7 0.5127275155528307 1.6131579030730498 8 0.36624168053729983 1.3168914280638564 9 0.26874218588553767 1.0871026899141683 >10 1.6111236671696085 13.878462411691512 >50 0.17961324325787315 5.6347310794783505 >100 0.12147159535392782 10.157273138017162 >500 0.00816433552049581 2.461012772192291 >1k 0.0013420825513143794 0.9367411456718686 >5k 1.1184021260953162E-4 0.3481550402427348 >10k+ 2.2368042521906325E-4 1.1900910450110809 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13523 0.6778592220283445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10152 0.5088831488598501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6926 0.347175402778105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2996 0.15017867553034978 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.12215802145108892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.0126393701718884E-5 0.0 2 0.0 0.0 0.0 5.0126393701718884E-5 0.0 3 0.0 0.0 0.0 5.0126393701718884E-5 0.0 4 0.0 0.0 0.0 5.0126393701718884E-5 0.0 5 0.0 0.0 0.0 5.0126393701718884E-5 0.0 6 0.0 0.0 0.0 5.0126393701718884E-5 0.0 7 0.0 0.0 0.0 5.0126393701718884E-5 0.0 8 0.0 0.0 0.0 5.0126393701718884E-5 0.0 9 0.0 0.0 0.0 5.0126393701718884E-5 0.0 10-11 0.0 0.0 0.0 5.0126393701718884E-5 0.0 12-13 0.0 0.0 0.0 1.0025278740343777E-4 0.0 14-15 0.0 0.0 0.0 1.7544237795601609E-4 0.0 16-17 0.0 0.0 0.0 2.506319685085944E-4 0.0 18-19 0.0 0.0 0.0 2.506319685085944E-4 0.0 20-21 0.0 0.0 0.0 3.007583622103133E-4 0.0 22-23 0.0 0.0 0.0 4.010111496137511E-4 0.0 24-25 0.0 0.0 0.0 8.52148692929221E-4 0.0 26-27 0.0 0.0 0.0 0.0011529070551395343 0.0 28-29 0.0 0.0 0.0 0.0029323940315505548 0.0 30-31 0.0 0.0 0.0 0.0077695910237664265 0.0 32-33 0.0 0.0 0.0 0.012556661622280581 0.0 34-35 0.0 0.0 0.0 0.017143226645987858 0.0 36-37 0.0 0.0 0.0 0.020401442236599586 0.0 38-39 0.0 0.0 0.0 0.02285763552798381 0.0 40-41 0.0 0.0 0.0 0.026767494236717884 0.0 42-43 0.0 0.0 0.0 0.0314543120478286 0.0 44-45 0.0 0.0 0.0 0.03644188822114963 0.0 46-47 0.0 0.0 0.0 0.04228161308739988 0.0 48-49 0.0 0.0 0.0 0.048346906725307864 0.0 50-51 0.0 0.0 0.0 0.058898512599519684 0.0 52-53 0.0 0.0 0.0 0.08260829682043272 0.0 54-55 0.0 0.0 0.0 0.11040338212803585 0.0 56-57 0.0 0.0 0.0 0.14381262353023147 0.0 58-59 0.0 0.0 0.0 0.18885118827122588 0.0 60-61 0.0 0.0 0.0 0.2560205558315292 0.0 62-63 0.0 0.0 0.0 0.3001819086827435 0.0 64-65 0.0 0.0 0.0 0.34070909799058324 0.0 66-67 0.0 0.0 0.0 0.42973357320483596 0.0 68-69 0.0 0.0 0.0 0.5515657730968637 0.0 70-71 0.0 0.0 0.0 0.6401140475709501 0.0 72-73 0.0 0.0 0.0 0.7197147607692798 0.0 74-75 0.0 0.0 0.0 0.8933275253551831 0.0 76-77 0.0 0.0 0.0 1.0080668405384174 0.0 78-79 0.0 0.0 0.0 1.0892966615320532 0.0 80-81 0.0 0.0 0.0 1.2016048466207543 0.0 82-83 0.0 0.0 0.0 1.4157698637113483 0.0 84-85 0.0 0.0 0.0 1.6317895573689056 0.0 86-87 0.0 0.0 0.0 1.9421220607762473 0.0 88-89 0.0 0.0 0.0 2.3339600803425835 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16640 0.0 30.263252 1 GGTATCA 11225 0.0 28.779657 1 CGATAGC 70 0.008395714 27.141075 4 GTACATG 11520 0.0 25.527084 1 TCAACGC 20270 0.0 24.603712 4 ATCAACG 20330 0.0 24.5311 3 TATCAAC 20495 0.0 24.427528 2 CAACGCA 20480 0.0 24.37462 5 AACGCAG 20860 0.0 23.957567 6 TACATGG 12125 0.0 23.93571 2 ACATGGG 11895 0.0 23.718493 3 GAGTACT 11335 0.0 22.798292 12-13 GTATAGG 1105 0.0 22.786448 1 CATATAG 610 0.0 22.580484 3 AGTACTT 11985 0.0 21.997831 12-13 CATGGGG 3910 0.0 21.622622 4 ACGCAGA 23045 0.0 21.562899 7 CGCAGAG 23105 0.0 21.445759 8 GTACTTT 12105 0.0 21.384655 14-15 GCAGAGT 24050 0.0 20.346287 9 >>END_MODULE