FastQCFastQC Report
Thu 26 May 2016
SRR937039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557845
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69931.2535740214575735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52470.9405838539379218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.5825991090715162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.2152927784599665No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA7160.1283510652600633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.1274547589384148No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6560.11759538940028144No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT6350.1138309028493578No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT6100.10934937124111535No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6100.10934937124111535No Hit
GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC6060.10863232618379658No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA5780.10361301078256505No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5610.10056556928896018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.050.4377561
GTACGGG554.9348164E-543.219081
GTATCAA50400.042.7302971
TACGGGA500.001608270838.0327952
CCCGCTA500.001611111238.0191359
TATCAAC64350.033.1715742
ATCAACG65750.032.606943
TCAACGC66000.032.483434
CAACGCA67150.031.992175
AACGCAG68350.031.4304926
ACGCAGA76050.028.1831537
CGCAGAG76150.028.021338
TACATGG38650.027.9218752
GTACATG39200.027.6513941
ACATGGG39700.026.462513
GCAGAGT79350.026.2324879
GTGGTAT12000.024.9590231
GAGTACT49750.024.92319912-13
TGGTATC12300.023.9637552
CATGGGG21700.023.658834
Produced by FastQC (version 0.11.5)