##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937039_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557845 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95256029900779 33.0 31.0 34.0 30.0 34.0 2 32.03142987747493 33.0 31.0 34.0 30.0 34.0 3 32.004157068719806 33.0 31.0 34.0 30.0 34.0 4 35.611035323432134 37.0 35.0 37.0 33.0 37.0 5 35.65940001254829 37.0 35.0 37.0 33.0 37.0 6 35.74788875045936 37.0 35.0 37.0 35.0 37.0 7 35.69733886653103 37.0 35.0 37.0 33.0 37.0 8 35.681969005727396 37.0 35.0 37.0 33.0 37.0 9 37.30196201453809 39.0 37.0 39.0 34.0 39.0 10-11 37.19539119289408 39.0 37.0 39.0 34.0 39.0 12-13 37.0972797103138 39.0 37.0 39.0 33.5 39.0 14-15 38.19308768564745 40.0 38.0 41.0 33.0 41.0 16-17 38.149622206885425 40.0 38.0 41.0 33.0 41.0 18-19 38.22880459625881 40.0 38.0 41.0 33.5 41.0 20-21 38.21711945074348 40.0 38.0 41.0 34.0 41.0 22-23 38.09198791779079 40.0 38.0 41.0 33.5 41.0 24-25 37.92572488773763 40.0 38.0 41.0 33.0 41.0 26-27 37.83080246304977 40.0 37.0 41.0 33.0 41.0 28-29 37.820291478815804 40.0 37.0 41.0 33.0 41.0 30-31 37.554632559223435 40.0 37.0 41.0 33.0 41.0 32-33 37.207295037151894 39.0 35.5 41.0 32.5 41.0 34-35 36.93635597701871 39.0 35.0 41.0 31.5 41.0 36-37 36.71195403741183 39.0 35.0 41.0 31.0 41.0 38-39 36.45595461104787 39.0 35.0 41.0 30.5 41.0 40-41 36.153781068217874 39.0 35.0 41.0 30.0 41.0 42-43 35.78461848721419 39.0 35.0 41.0 29.5 41.0 44-45 35.25081698321218 39.0 35.0 40.5 26.5 41.0 46-47 34.52066972008353 38.0 34.5 40.0 22.0 41.0 48-49 33.88180229275157 38.0 34.0 40.0 12.5 41.0 50-51 33.099040055929514 37.5 33.5 39.5 7.5 40.5 52-53 33.327439521730945 38.0 33.5 40.0 4.5 40.5 54-55 33.563225447929085 39.0 34.0 40.0 2.0 41.0 56-57 33.42673143973684 38.0 34.0 41.0 2.0 41.0 58-59 33.19590836164167 38.0 34.0 40.0 2.0 41.0 60-61 32.92153465568393 38.0 33.0 40.0 2.0 41.0 62-63 32.62875081787952 37.5 33.0 40.0 2.0 41.0 64-65 32.32517993349407 37.0 33.0 40.0 2.0 41.0 66-67 31.962394571968915 36.0 32.0 39.5 2.0 41.0 68-69 31.514184047540088 36.0 32.0 39.0 2.0 41.0 70-71 31.183506171069027 35.0 31.0 39.0 2.0 40.5 72-73 30.827975512911294 35.0 31.0 38.0 2.0 40.0 74-75 30.416022371805788 35.0 31.0 37.0 2.0 39.5 76-77 30.02458568240282 35.0 30.5 37.0 2.0 39.0 78-79 29.61529994891054 35.0 30.0 36.0 2.0 39.0 80-81 29.206117290645253 35.0 29.5 36.0 2.0 37.5 82-83 28.83136803233873 34.0 29.0 35.5 2.0 37.0 84-85 28.5101228835967 34.0 29.0 35.0 2.0 37.0 86-87 28.207875843648324 34.0 29.0 35.0 2.0 36.0 88-89 27.861224892219163 34.0 28.5 35.0 2.0 36.0 90-91 27.5499565291434 34.0 27.0 35.0 2.0 36.0 92-93 27.2433444774086 34.0 26.5 35.0 2.0 35.0 94-95 26.885965635615626 34.0 25.5 35.0 2.0 35.0 96-97 26.50999919332431 34.0 25.0 35.0 2.0 35.0 98-99 26.009333237727326 34.0 23.5 35.0 2.0 35.0 100-101 24.18293343132949 31.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11098084234104633 1101 2 0.06068580462083162 1101 3 0.3412215007262773 1101 4 0.17856043080100648 1101 5 -0.21025175686509812 1101 6 0.07046972677557761 1101 7 0.1054982403510678 1101 8 -0.06257997420237871 1101 9 0.1614565753552526 1101 10-11 -0.00795724096573025 1101 12-13 0.23705358923992748 1101 14-15 0.3585724164068864 1101 16-17 0.37906470492904276 1101 18-19 0.503081327151925 1101 20-21 0.25836917435847795 1101 22-23 0.25720935541307455 1101 24-25 0.46330034337054116 1101 26-27 0.3946311069624997 1101 28-29 0.31628662809174557 1101 30-31 0.2622577256688672 1101 32-33 0.31871739435128177 1101 34-35 0.4050352570083078 1101 36-37 0.3792379030230677 1101 38-39 0.5235117886416134 1101 40-41 0.5597993404747186 1101 42-43 0.7205715001069208 1101 44-45 0.7103546441468467 1101 46-47 0.5450724289257138 1101 48-49 0.5834677278927174 1101 50-51 0.704370780542142 1101 52-53 0.8864748650946055 1101 54-55 0.7893355111056835 1101 56-57 0.8493562666110606 1101 58-59 0.69259895548619 1101 60-61 0.9508302911784057 1101 62-63 0.8913184519357742 1101 64-65 0.7208868877132915 1101 66-67 0.9262871110950002 1101 68-69 1.1271386560091088 1101 70-71 0.9882725772470806 1101 72-73 1.232098044198711 1101 74-75 1.1458566729387378 1101 76-77 1.0971035206236515 1101 78-79 1.1282008815002804 1101 80-81 1.1541031668336146 1101 82-83 0.8857033125644733 1101 84-85 1.0429247741571892 1101 86-87 1.3071105418647484 1101 88-89 1.4549069666018326 1101 90-91 1.298962099975018 1101 92-93 1.1558271237744329 1101 94-95 1.3136781114621812 1101 96-97 1.507263289464511 1101 98-99 1.1961852719103305 1101 100-101 1.5268773567741256 1102 1 0.34204094996548307 1102 2 0.3176304895388711 1102 3 0.26692324113946597 1102 4 0.3418877849989812 1102 5 0.13598173414195713 1102 6 0.2461074050242189 1102 7 0.12428403724976533 1102 8 0.18562109096096435 1102 9 0.19463038812752131 1102 10-11 0.23468253575166642 1102 12-13 0.32044521337162735 1102 14-15 0.3761670033271187 1102 16-17 0.18354524734511557 1102 18-19 0.27811231938828485 1102 20-21 0.20517160664303447 1102 22-23 0.3689597415174646 1102 24-25 0.4251746984203706 1102 26-27 0.4531285202361701 1102 28-29 0.5371331489967872 1102 30-31 0.4587727439179332 1102 32-33 0.6569846686273735 1102 34-35 0.5889611942204667 1102 36-37 0.779136442405715 1102 38-39 0.6456367825816756 1102 40-41 0.7746153608637982 1102 42-43 0.9937654664898687 1102 44-45 0.9639940676566283 1102 46-47 0.6811872144732902 1102 48-49 0.6839033506115797 1102 50-51 0.5681140193866696 1102 52-53 0.5523629744643088 1102 54-55 0.6708603291345057 1102 56-57 0.8172818990950859 1102 58-59 0.775800124534122 1102 60-61 0.7054912465011043 1102 62-63 0.8292370202675983 1102 64-65 0.8617041824200271 1102 66-67 0.8225999006732962 1102 68-69 0.7259640675652292 1102 70-71 0.6733793359785807 1102 72-73 0.7537164276159594 1102 74-75 0.9276921058950869 1102 76-77 0.943172527694724 1102 78-79 0.9164373366810068 1102 80-81 0.8891074261018481 1102 82-83 0.957229584977572 1102 84-85 0.8662586384562125 1102 86-87 1.0419866768039086 1102 88-89 1.0598840773635807 1102 90-91 1.0317986341080285 1102 92-93 0.7514719354316703 1102 94-95 0.7916511153197661 1102 96-97 0.674801432423557 1102 98-99 0.6530420012078615 1102 100-101 1.0184124940056698 1103 1 0.4737201227197083 1103 2 0.4766008295436919 1103 3 0.3759389718590498 1103 4 0.321238555931906 1103 5 0.2571146580132222 1103 6 0.14152081514351522 1103 7 0.286977834324901 1103 8 0.39425880213417486 1103 9 0.3947715248812287 1103 10-11 0.4444833839906366 1103 12-13 0.2194278230540334 1103 14-15 0.34774284128227606 1103 16-17 0.48216295823463184 1103 18-19 0.6315038568569022 1103 20-21 0.5078124242432906 1103 22-23 0.27544423065915424 1103 24-25 0.6188884390408091 1103 26-27 0.801931340134324 1103 28-29 0.8250131189254404 1103 30-31 0.9310524925668489 1103 32-33 0.7215653718997572 1103 34-35 0.61662648025289 1103 36-37 0.9020122988008481 1103 38-39 1.018663350334407 1103 40-41 1.2375492322662396 1103 42-43 1.1854543556939632 1103 44-45 1.5407238794270057 1103 46-47 1.2960026728485872 1103 48-49 1.2861317510144303 1103 50-51 1.4398568809875414 1103 52-53 1.3174500678982497 1103 54-55 1.3605314592214555 1103 56-57 1.3453106654605662 1103 58-59 1.2883215787391507 1103 60-61 1.1450435805767682 1103 62-63 1.1911659128760022 1103 64-65 1.1975103640679876 1103 66-67 1.2273964226097718 1103 68-69 1.4784375069047968 1103 70-71 1.4409540533504526 1103 72-73 1.5428605998737233 1103 74-75 1.5111720134115068 1103 76-77 1.6561329952190889 1103 78-79 1.5643578745609794 1103 80-81 1.661843564203096 1103 82-83 1.436700924996579 1103 84-85 1.459623437956914 1103 86-87 1.5947374250414725 1103 88-89 1.5685154054675259 1103 90-91 1.5687991589381696 1103 92-93 1.492624292202688 1103 94-95 1.4073779341398875 1103 96-97 1.252564946799648 1103 98-99 1.1122346847920248 1103 100-101 1.1213643089250276 1104 1 0.33829231523307257 1104 2 0.43609280815331886 1104 3 0.3684523408430067 1104 4 0.398511283204634 1104 5 0.2607242836816681 1104 6 0.527216002309288 1104 7 0.43884949207890855 1104 8 0.48476682352454503 1104 9 0.37605494734112455 1104 10-11 0.47436306313502286 1104 12-13 0.6293877161021655 1104 14-15 0.7194674402127532 1104 16-17 0.6041549368442602 1104 18-19 0.6192712365360435 1104 20-21 0.5217830124785863 1104 22-23 0.6122757814612996 1104 24-25 0.330185230484652 1104 26-27 0.5815436395995661 1104 28-29 0.41966552534255186 1104 30-31 0.5309856476470571 1104 32-33 0.7309236606698164 1104 34-35 0.8142869080603745 1104 36-37 0.9103679137741025 1104 38-39 0.7122462380349432 1104 40-41 0.6656240985763944 1104 42-43 0.7046388476725767 1104 44-45 0.5645206708708628 1104 46-47 0.7432620311373555 1104 48-49 0.7124686494101482 1104 50-51 0.9516215868698907 1104 52-53 1.1058190518554696 1104 54-55 1.1136731704514062 1104 56-57 1.170578045139706 1104 58-59 1.0809686375626768 1104 60-61 1.1846826180099157 1104 62-63 1.2193076241059515 1104 64-65 1.2069354977578328 1104 66-67 1.038358989454693 1104 68-69 1.096285100487684 1104 70-71 0.998574374206072 1104 72-73 1.2031547175207784 1104 74-75 1.247000890416853 1104 76-77 1.2249832625987693 1104 78-79 0.8957749868604417 1104 80-81 1.0213355428127215 1104 82-83 1.1645084116275939 1104 84-85 1.0927116732510314 1104 86-87 0.9789620239719561 1104 88-89 1.175266742365924 1104 90-91 1.18524300920555 1104 92-93 1.129723222683971 1104 94-95 0.9994233886853436 1104 96-97 0.7778323264787943 1104 98-99 0.750403133989888 1104 100-101 1.233997998764604 1105 1 0.45564435558980065 1105 2 0.45262059844247915 1105 3 0.3894144163317037 1105 4 0.39931301277854203 1105 5 0.23484598442934868 1105 6 0.28610033705390947 1105 7 0.34332206211765026 1105 8 0.3604964875187804 1105 9 0.5339010873285162 1105 10-11 0.5025575951591676 1105 12-13 0.3657076422349448 1105 14-15 0.5109580716873907 1105 16-17 0.6504852224041215 1105 18-19 0.5755916130787 1105 20-21 0.6255157843009371 1105 22-23 0.6797280380147583 1105 24-25 0.6440837531402721 1105 26-27 0.5950007605796586 1105 28-29 0.5200979188206176 1105 30-31 0.40601379826411943 1105 32-33 0.6499512257540374 1105 34-35 0.6178169954398882 1105 36-37 0.5710009648505832 1105 38-39 0.5336947561864633 1105 40-41 0.7070370343479411 1105 42-43 0.8235797088562506 1105 44-45 0.8906872324418913 1105 46-47 1.013391974385712 1105 48-49 1.254386044239446 1105 50-51 0.8967076151556341 1105 52-53 1.1628274294791154 1105 54-55 1.4094449477687618 1105 56-57 1.478593088829463 1105 58-59 1.320841171568624 1105 60-61 1.1938270644561868 1105 62-63 1.1630818336366175 1105 64-65 1.3802047978208876 1105 66-67 1.600947945404613 1105 68-69 1.5489256283680533 1105 70-71 1.597252421228859 1105 72-73 1.6310170722635924 1105 74-75 1.5806374997313277 1105 76-77 1.5874393476361526 1105 78-79 1.6677392243165272 1105 80-81 1.4751640267330899 1105 82-83 1.6568469396881298 1105 84-85 1.5737685600179887 1105 86-87 1.5239196854438966 1105 88-89 1.5361677873168276 1105 90-91 1.6660037514810213 1105 92-93 1.9569538775799273 1105 94-95 1.7190195962342116 1105 96-97 1.923366512588153 1105 98-99 2.1485981164239014 1105 100-101 2.264406295314462 1106 1 0.5796036432577374 1106 2 0.40762504550240664 1106 3 0.39172488130023453 1106 4 0.479477119320741 1106 5 0.38117766153936117 1106 6 0.3828222185949315 1106 7 0.3941924152743894 1106 8 0.3945215114259071 1106 9 0.2982871590030811 1106 10-11 0.5150996416092113 1106 12-13 0.4194050549154156 1106 14-15 0.6627984188924358 1106 16-17 0.6552883814884893 1106 18-19 0.720147809872806 1106 20-21 0.6160515714305461 1106 22-23 0.5290462669480647 1106 24-25 0.556459954348 1106 26-27 0.5465545566301344 1106 28-29 0.4388538120109331 1106 30-31 0.06994981409966528 1106 32-33 0.3002468047746163 1106 34-35 0.3812885777238719 1106 36-37 0.27793470031404865 1106 38-39 0.08488944378886032 1106 40-41 -0.0156820920221179 1106 42-43 0.23956371281514066 1106 44-45 0.31319136185467755 1106 46-47 -0.0025605992248287635 1106 48-49 0.017304251773367696 1106 50-51 0.0978969529115119 1106 52-53 -0.18366206810342334 1106 54-55 0.0648579893571295 1106 56-57 0.21349481883608235 1106 58-59 0.18859129361199223 1106 60-61 0.03824349650979286 1106 62-63 -0.0758021883779989 1106 64-65 -0.09893080072662741 1106 66-67 0.08431326636196701 1106 68-69 0.07918068230119246 1106 70-71 -0.23921781690618005 1106 72-73 -0.17756709085322697 1106 74-75 -0.09955703406298255 1106 76-77 -0.08952354449088062 1106 78-79 0.13652119800865492 1106 80-81 0.11762375241971057 1106 82-83 -0.048669086445869425 1106 84-85 0.16964784073112682 1106 86-87 0.2768621010333483 1106 88-89 0.19272435860019144 1106 90-91 0.38522374385749103 1106 92-93 0.5295145147466478 1106 94-95 0.5889012977528978 1106 96-97 0.5945482268127265 1106 98-99 0.8295098640181422 1106 100-101 0.7878285921373198 1107 1 0.1597330654462077 1107 2 0.10258897183618387 1107 3 0.3085418549094996 1107 4 0.5574626898542476 1107 5 0.20865693500307003 1107 6 0.3334819869640171 1107 7 0.2928776250712417 1107 8 0.324003822672033 1107 9 0.16580345544001318 1107 10-11 0.4834956292049313 1107 12-13 0.592388568617082 1107 14-15 0.30829949477371343 1107 16-17 0.3689141013796373 1107 18-19 0.38591659032973524 1107 20-21 0.5022177950872759 1107 22-23 0.4022267618534556 1107 24-25 0.4539703967250617 1107 26-27 0.24712334974449846 1107 28-29 0.27762588339881944 1107 30-31 0.47706407901961256 1107 32-33 0.5361772838582226 1107 34-35 0.8103120572504849 1107 36-37 0.717859388624909 1107 38-39 0.7394840896973491 1107 40-41 0.9842941753027361 1107 42-43 0.9244491631030982 1107 44-45 0.6021485481087083 1107 46-47 0.8485049978892718 1107 48-49 0.6595381293760525 1107 50-51 0.4243517658980238 1107 52-53 0.5329183698435358 1107 54-55 0.8032724884394682 1107 56-57 0.66632612698114 1107 58-59 0.6513863698729878 1107 60-61 0.6072422940542523 1107 62-63 0.5765220316080786 1107 64-65 0.7460552761039523 1107 66-67 0.6351407456354252 1107 68-69 0.6159781951168384 1107 70-71 0.48420949782073563 1107 72-73 0.33479367745257704 1107 74-75 0.38828392536996503 1107 76-77 0.3895719241503457 1107 78-79 0.7139015853259174 1107 80-81 0.49760365875475543 1107 82-83 0.7175561524630609 1107 84-85 0.7675411702672292 1107 86-87 0.8932629617383654 1107 88-89 0.7764176375065865 1107 90-91 0.82758103137094 1107 92-93 0.915166530441855 1107 94-95 0.9024178473383699 1107 96-97 0.5590876546970378 1107 98-99 0.7494120853965391 1107 100-101 0.9129195673920556 1108 1 -0.005601004806806031 1108 2 0.05695522091503591 1108 3 -0.00562542585689485 1108 4 0.08564986745189174 1108 5 0.13926366553610592 1108 6 0.11338250280779505 1108 7 0.046875414312211205 1108 8 0.04381467995525412 1108 9 -0.18511155315737682 1108 10-11 0.017645715178893795 1108 12-13 -0.1792737993514777 1108 14-15 0.21858259474914377 1108 16-17 0.242876272836277 1108 18-19 0.1866565805041418 1108 20-21 0.19996097858027184 1108 22-23 0.04897817428282991 1108 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-1.3814066475025584 2316 92-93 -1.7498857029051926 2316 94-95 -1.7331545478520454 2316 96-97 -1.7315989223614388 2316 98-99 -1.9139571369323605 2316 100-101 -1.8803901066352857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1261.0 3 336.0 4 297.0 5 296.0 6 372.0 7 496.0 8 620.0 9 814.0 10 1116.0 11 1756.0 12 2744.0 13 3877.0 14 4553.0 15 6185.0 16 8957.0 17 12673.0 18 10453.0 19 5167.0 20 3816.0 21 3471.0 22 3450.0 23 3797.0 24 4171.0 25 4724.0 26 5364.0 27 6114.0 28 7439.0 29 8894.0 30 10954.0 31 13471.0 32 17520.0 33 23140.0 34 32563.0 35 46762.0 36 69063.0 37 105599.0 38 105044.0 39 20511.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.378699286149875 19.505326972055816 15.426157764465328 20.689815977328983 2 15.461407484358991 20.816400495919073 41.54793495637384 22.174257063348097 3 18.023559293759316 26.787530471562565 33.08991958556881 22.098990649109307 4 11.621458147494701 18.150474101647823 40.08568469682802 30.14238305402946 5 11.66489316841295 38.561474593515996 34.84035824066581 14.93327399740525 6 26.96731430376022 38.61455425007357 19.326655996325194 15.091475449841022 7 24.937597685865583 36.545773959168876 21.481828410234876 17.03479994473066 8 26.003602814788767 32.598117493388266 20.92126557840608 20.477014113416896 9 27.75440838139812 15.44952389839816 22.002605727575848 34.79346199262787 10-11 23.774993382949994 26.8290647753119 28.554073635429706 20.841868206308405 12-13 25.60673112030738 26.080848441243994 28.842032361983417 19.470388076465213 14-15 22.732761559578705 25.78292932373997 29.835465523119158 21.648843593562162 16-17 19.49479959229378 29.071050704872377 29.733483591260157 21.70066611157369 18-19 19.372539310482168 26.40406753266791 34.84556131753468 19.37783183931524 20-21 21.006898976454508 25.00627831970639 35.129082253163375 18.857740450675728 22-23 20.442033054263455 25.174590588881095 34.85622848450166 19.527147872353783 24-25 19.88267538156061 25.651419787520652 35.28258533881721 19.183319492101532 26-27 19.747749945295602 25.76595126465809 35.332584809754245 19.153713980292068 28-29 19.304824345049642 26.145636205458445 35.22426612375221 19.325273325739705 30-31 19.988163027395416 25.55001569295611 35.36152087163162 19.10030040801686 32-33 19.43603240863214 25.735269720706462 35.201746873696905 19.626950996964492 34-35 19.760131636171252 25.8908083339685 34.7947219992916 19.554338030568644 36-37 20.235720972943113 25.434388036573246 34.60496156508826 19.72492942539538 38-39 19.511628636891498 26.349903823303904 34.50783985795442 19.63062768185018 40-41 19.940645154346814 26.178464540905782 34.33881410489251 19.542076199854886 42-43 19.79121825226001 26.25896828813316 33.77080642846894 20.179007031137896 44-45 20.262330539708273 26.336537064702938 33.14463062633277 20.256501769256015 46-47 20.37737067110435 26.690672252029955 32.33258256104209 20.5993745158236 48-49 20.632347270917585 27.024058249118998 31.84933779288206 20.494256687081357 50-51 20.739274388981084 27.50900805188494 30.927450553214076 20.824267005919896 52-53 21.010794976153136 27.65635409745517 30.4523416911681 20.880509235223595 54-55 21.28214003253234 27.236957024671852 30.236065703130745 21.244837239665067 56-57 21.376917516953423 27.989066588707523 29.66557679304537 20.96843910129368 58-59 21.252819291802865 27.677172592223908 29.821493428752067 21.248514687221153 60-61 21.215354967263934 27.323247223868762 30.526637677546404 20.934760131320896 62-63 21.085148820307147 27.045853228057208 30.29902173562109 21.56997621601455 64-65 21.132534636259866 27.035247342931925 30.783791369157836 21.048426651650374 66-67 21.180462132871273 27.561605160160862 29.91863706318542 21.339295643782442 68-69 21.158447344443886 27.213890104529114 30.152202142237083 21.475460408789914 70-71 20.66326644964807 27.580308386355263 30.662737500437053 21.093687663559614 72-73 21.271504773858084 27.184454704379394 30.37715720112959 21.16688332063293 74-75 20.586244336913417 27.61524095407935 30.132148399990317 21.666366309016915 76-77 20.290543830404612 27.611886095914794 30.312700913452872 21.78486916022772 78-79 20.36052308942404 27.655828612069516 30.057641720874877 21.92600657763157 80-81 20.158710232775007 27.199078225314732 30.345037839388834 22.29717370252143 82-83 20.368681283693917 27.893966014380254 30.08840350815829 21.64894919376754 84-85 20.406911303739513 27.373103674040454 29.648560294533112 22.57142472768692 86-87 20.19801589472636 27.508316434386494 29.645162476535052 22.64850519435209 88-89 20.39988086822662 27.12050968304489 29.828066297549544 22.651543151178938 90-91 19.98564996681555 26.89492188200685 30.565570682882203 22.553857468295398 92-93 20.153306054207846 27.00875598935863 30.56236736407228 22.275570592361245 94-95 19.888502179724078 27.53511777686084 30.089790280045207 22.48658976336987 96-97 20.0711743133751 27.437365886566052 29.730481337151303 22.760978462907545 98-99 19.952117001066956 27.122003835306174 29.676780783626455 23.24909838000041 100-101 20.25752728936825 27.398480858185014 29.761361036210847 22.582630816235884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 437.0 1 300.0 2 170.0 3 181.5 4 175.5 5 199.5 6 315.5 7 586.0 8 949.0 9 1117.5 10 995.5 11 902.5 12 959.0 13 1047.5 14 1143.5 15 1245.0 16 1375.0 17 1502.5 18 1692.5 19 1908.5 20 2117.5 21 2397.0 22 2729.0 23 3089.0 24 3489.0 25 4276.5 26 6160.0 27 8276.5 28 8772.0 29 9435.0 30 11128.0 31 12451.5 32 13901.5 33 15652.5 34 18057.5 35 19573.0 36 20011.5 37 20522.5 38 18231.5 39 15351.5 40 14936.0 41 15169.0 42 15475.0 43 16242.5 44 16995.0 45 16547.5 46 16160.5 47 16491.0 48 17562.5 49 17974.5 50 16866.5 51 15585.5 52 14789.5 53 14354.5 54 13254.0 55 12140.0 56 11342.5 57 10591.0 58 10018.5 59 9236.5 60 7988.5 61 6521.0 62 5138.0 63 3678.5 64 2519.5 65 1983.0 66 1555.0 67 1097.0 68 768.5 69 560.5 70 417.0 71 311.0 72 231.0 73 214.0 74 163.0 75 120.5 76 88.5 77 44.0 78 28.5 79 16.5 80 10.0 81 13.5 82 10.0 83 4.5 84 5.0 85 4.0 86 3.5 87 3.0 88 2.0 89 2.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054674685620557675 2 0.08891358710752986 3 0.06417553263003165 4 0.12297322733017235 5 0.10146187561060867 6 0.09626329894504747 7 0.10289596572524626 8 0.09016841595783774 9 0.10952863250544506 10-11 0.10262707382875172 12-13 0.08846543394670563 14-15 0.09231955112979412 16-17 0.10415079457555415 18-19 0.08165350590217713 20-21 0.0665059290663177 22-23 0.07134598320321954 24-25 0.05951473975745951 26-27 0.05431616309189829 28-29 0.06453405515869104 30-31 0.04840054136901828 32-33 0.049745000851491014 34-35 0.04436716292160008 36-37 0.05001389274798555 38-39 0.049745000851491014 40-41 0.06283107314755891 42-43 0.05825991090715163 44-45 0.04768349631169949 46-47 0.03531446907295037 48-49 0.043022703439127355 50-51 0.02679955901728975 52-53 0.04024415384201705 54-55 0.044098271025105544 56-57 0.05162724412695283 58-59 0.05422653245973344 60-61 0.0501035233801504 62-63 0.058618433435811024 64-65 0.04096119889933584 66-67 0.061486613665086176 68-69 0.0532405955059201 70-71 0.02428990131667399 72-73 0.021511351719563677 74-75 0.03163961315419157 76-77 0.04221602774964372 78-79 0.06265181188322921 80-81 0.04033378447418189 82-83 0.06175550556158073 84-85 0.0631895956762183 86-87 0.06534073084817467 88-89 0.08586614561392501 90-91 0.06363774883704255 92-93 0.07134598320321954 94-95 0.07797864998341833 96-97 0.0862246681425844 98-99 0.1171472362394572 100-101 0.11159013704523657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557845.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.37589396028848 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34311221170185 44.4907166227476 2 11.868654429459498 13.144746982741145 3 3.5167221872532295 5.8422490478738585 4 1.3791642336763954 3.0548980943154636 5 0.7124671381538551 1.9726752346299041 6 0.44845222331884543 1.4900065658855997 7 0.2758634363045618 1.0693329077425546 8 0.20142043628064155 0.8923069376729478 9 0.1450739691712626 0.7230240659903399 >10 0.9094181755002775 10.093113507524109 >50 0.11293094769969164 4.36573011896736 >100 0.08089609279836055 8.381864832781991 >500 0.004530181196708185 1.4885125417602654 >1k 6.471687423868835E-4 0.7975646230137164 >5k 6.471687423868835E-4 2.193257916353154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 1.2535740214575735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5247 0.9405838539379218 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3250 0.5825991090715162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.2152927784599665 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 716 0.1283510652600633 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.1274547589384148 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 656 0.11759538940028144 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 635 0.1138309028493578 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 610 0.10934937124111535 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 610 0.10934937124111535 No Hit GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC 606 0.10863232618379658 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 578 0.10361301078256505 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 561 0.10056556928896018 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.7926126432969732E-4 0.0 0.0 0.0 0.0 8 1.7926126432969732E-4 0.0 0.0 0.0 0.0 9 1.7926126432969732E-4 0.0 0.0 1.7926126432969732E-4 0.0 10-11 1.7926126432969732E-4 0.0 0.0 1.7926126432969732E-4 0.0 12-13 1.7926126432969732E-4 0.0 0.0 1.7926126432969732E-4 0.0 14-15 1.7926126432969732E-4 0.0 0.0 1.7926126432969732E-4 0.0 16-17 1.7926126432969732E-4 0.0 0.0 3.5852252865939464E-4 0.0 18-19 1.7926126432969732E-4 0.0 0.0 7.170450573187893E-4 0.0 20-21 1.7926126432969732E-4 0.0 0.0 7.170450573187893E-4 0.0 22-23 1.7926126432969732E-4 0.0 0.0 0.0010755675859781839 0.0 24-25 1.7926126432969732E-4 0.0 0.0 0.0019718739076266703 0.0 26-27 1.7926126432969732E-4 0.0 0.0 0.0033163333900994006 0.0 28-29 1.7926126432969732E-4 0.0 0.0 0.005467468562055768 0.0 30-31 1.7926126432969732E-4 0.0 0.0 0.010755675859781839 0.0 32-33 1.7926126432969732E-4 0.0 0.0 0.0167609282148267 0.0 34-35 1.7926126432969732E-4 0.0 0.0 0.022945441834201257 0.0 36-37 1.7926126432969732E-4 0.0 0.0 0.02948847798223521 0.0 38-39 1.7926126432969732E-4 0.0 0.0 0.03585225286593946 0.0 40-41 1.7926126432969732E-4 0.0 0.0 0.04607014493273221 0.0 42-43 1.7926126432969732E-4 0.0 0.0 0.06516146958384497 0.0 44-45 1.7926126432969732E-4 0.0 0.0 0.09214028986546441 0.0 46-47 1.7926126432969732E-4 0.0 0.0 0.11822280382543539 0.0 48-49 1.7926126432969732E-4 0.0 0.0 0.16644408393012397 0.0 50-51 1.7926126432969732E-4 0.0 0.0 0.22318027409047314 0.0 52-53 1.7926126432969732E-4 0.0 0.0 0.28610097787019695 0.0 54-55 1.7926126432969732E-4 0.0 0.0 0.404503042959962 0.0 56-57 1.7926126432969732E-4 0.0 0.0 0.5148383511548906 0.0 58-59 1.7926126432969732E-4 0.0 0.0 0.6630874167555504 0.0 60-61 1.7926126432969732E-4 0.0 0.0 0.8256773835025859 0.0 62-63 1.7926126432969732E-4 0.0 0.0 1.0402531169052334 0.0 64-65 1.7926126432969732E-4 0.0 0.0 1.2066075702031926 0.0 66-67 1.7926126432969732E-4 0.0 0.0 1.4270092946965556 0.0 68-69 1.7926126432969732E-4 0.0 0.0 1.7182192186001488 0.0 70-71 1.7926126432969732E-4 0.0 0.0 1.8981975279871648 0.0 72-73 1.7926126432969732E-4 0.0 0.0 2.1780243616058224 0.0 74-75 1.7926126432969732E-4 0.0 0.0 2.530989791070996 0.0 76-77 1.7926126432969732E-4 0.0 0.0 2.7902015792917387 0.0 78-79 1.7926126432969732E-4 0.0 0.0 3.013919637175201 0.0 80-81 1.7926126432969732E-4 0.0 0.0 3.366885066640375 0.0 82-83 1.7926126432969732E-4 0.0 0.0 3.80804703815576 0.0 84-85 1.7926126432969732E-4 0.0 0.0 4.336419614767543 0.0 86-87 1.7926126432969732E-4 0.0 0.0 5.074527870645071 0.0 88-89 1.7926126432969732E-4 0.0 0.0 5.842931280194319 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2790 0.0 50.437756 1 GTACGGG 55 4.9348164E-5 43.21908 1 GTATCAA 5040 0.0 42.730297 1 TACGGGA 50 0.0016082708 38.032795 2 CCCGCTA 50 0.0016111112 38.019135 9 TATCAAC 6435 0.0 33.171574 2 ATCAACG 6575 0.0 32.60694 3 TCAACGC 6600 0.0 32.48343 4 CAACGCA 6715 0.0 31.99217 5 AACGCAG 6835 0.0 31.430492 6 ACGCAGA 7605 0.0 28.183153 7 CGCAGAG 7615 0.0 28.02133 8 TACATGG 3865 0.0 27.921875 2 GTACATG 3920 0.0 27.651394 1 ACATGGG 3970 0.0 26.46251 3 GCAGAGT 7935 0.0 26.232487 9 GTGGTAT 1200 0.0 24.959023 1 GAGTACT 4975 0.0 24.923199 12-13 TGGTATC 1230 0.0 23.963755 2 CATGGGG 2170 0.0 23.65883 4 >>END_MODULE