FastQCFastQC Report
Thu 26 May 2016
SRR937039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557845
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56971.0212514228862857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.8312344826968064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.5186028377058143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.19270585915442462No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9370.16796780467692637No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7760.1391067411198451No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6750.12100135342254568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.11508573169966567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6260.11221755147039052No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.11024567756276385No Hit
ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT5900.10576414595452141No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5740.10289596572524626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5670.10164113687493838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.047.7301861
GTATCAA52400.040.0673981
CCGACCA702.0528812E-433.926049
TACACGG851.630534E-533.529922
GTACACG902.4105046E-531.6671451
GATAGTC753.077777E-431.6643077
TATCAAC68350.030.5783672
ATCAACG68900.030.4004943
TCAACGC69150.030.3592684
CAACGCA70600.029.7357435
TACGACG650.00584365529.228595
AACGCAG72100.029.0512336
CGGTGTA908.9456455E-426.386929
ACGCAGA81600.025.6108367
CGCAGAG81650.025.478818
GTACATG44600.024.4958861
TACATGG43750.024.3203682
GCAGAGT85100.023.9435739
CGTAACG500.001653469723.7503678-79
GATCGTC1000.001653299323.748237
Produced by FastQC (version 0.11.5)