##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937039_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557845 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.105290896216694 33.0 31.0 34.0 31.0 34.0 2 32.08307684034096 33.0 31.0 34.0 30.0 34.0 3 32.23477130744203 33.0 31.0 34.0 30.0 34.0 4 36.00666672642042 37.0 35.0 37.0 35.0 37.0 5 35.96197510061038 37.0 35.0 37.0 35.0 37.0 6 36.02353521139385 37.0 35.0 37.0 35.0 37.0 7 35.963566940637634 37.0 35.0 37.0 35.0 37.0 8 35.95671736772759 37.0 35.0 37.0 35.0 37.0 9 37.60926242952792 39.0 37.0 39.0 35.0 39.0 10-11 37.54898941462234 39.0 37.0 39.0 35.0 39.0 12-13 37.44768170369906 39.0 37.0 39.0 34.5 39.0 14-15 38.57517052227769 40.0 38.0 41.0 34.0 41.0 16-17 38.51750575876812 40.0 38.0 41.0 34.0 41.0 18-19 38.66796870098325 40.0 38.0 41.0 34.5 41.0 20-21 38.76002473805448 40.0 38.0 41.0 35.0 41.0 22-23 38.662966415402124 40.0 38.0 41.0 34.0 41.0 24-25 38.5450089182479 40.0 38.0 41.0 34.0 41.0 26-27 38.35402665615001 40.0 38.0 41.0 34.0 41.0 28-29 38.149654473913 40.0 37.5 41.0 34.0 41.0 30-31 37.86914913640886 40.0 37.0 41.0 33.0 41.0 32-33 37.67662791635669 40.0 36.0 41.0 33.0 41.0 34-35 37.489878012709624 40.0 36.0 41.0 33.0 41.0 36-37 37.251302781238515 40.0 35.5 41.0 33.0 41.0 38-39 37.00371339709059 40.0 35.0 41.0 32.0 41.0 40-41 36.73372352535202 39.0 35.0 41.0 31.0 41.0 42-43 36.35156002115283 39.0 35.0 41.0 30.5 41.0 44-45 35.739242083374414 39.0 35.0 41.0 29.0 41.0 46-47 35.10620961019638 39.0 35.0 41.0 25.0 41.0 48-49 34.49111670804614 39.0 35.0 40.5 21.0 41.0 50-51 34.11139115704183 39.0 34.0 40.0 16.5 41.0 52-53 33.82526328998198 38.0 34.0 40.0 10.5 41.0 54-55 33.540405488979914 38.0 34.0 40.0 7.5 41.0 56-57 33.24218017549678 38.0 33.0 40.0 4.5 41.0 58-59 32.92950550780235 37.5 33.0 40.0 2.0 41.0 60-61 32.608024630497724 37.0 33.0 40.0 2.0 41.0 62-63 32.23716623793348 36.5 32.0 39.5 2.0 41.0 64-65 31.85377927560523 36.0 32.0 39.0 2.0 40.5 66-67 31.48806568132725 35.5 31.0 39.0 2.0 40.0 68-69 31.026056521076644 35.0 31.0 38.5 2.0 40.0 70-71 30.57091127463722 35.0 30.5 38.0 2.0 40.0 72-73 30.25198397404297 35.0 30.0 37.0 2.0 39.0 74-75 29.820990597746686 35.0 30.0 37.0 2.0 39.0 76-77 28.458403319918617 33.5 28.0 35.0 2.0 37.5 78-79 29.149866898511235 34.0 29.0 36.0 2.0 38.0 80-81 28.974708028215723 34.0 29.0 35.0 2.0 37.0 82-83 28.668925059828446 34.0 29.0 35.0 2.0 37.0 84-85 28.32334519445366 34.0 29.0 35.0 2.0 36.0 86-87 27.988965572874186 34.0 29.0 35.0 2.0 36.0 88-89 27.64536654447024 34.0 27.5 35.0 2.0 35.5 90-91 27.275817655441923 34.0 26.5 35.0 2.0 35.0 92-93 26.863183321531967 33.0 25.5 35.0 2.0 35.0 94-95 26.43524903871147 33.0 25.0 35.0 2.0 35.0 96-97 25.868033235038407 33.0 24.0 35.0 2.0 35.0 98-99 25.24687413170325 33.0 19.0 34.5 2.0 35.0 100-101 22.64032840663625 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.009067859884858365 1101 2 0.058578484865535074 1101 3 0.08658237275641767 1101 4 0.03155060209281402 1101 5 0.041492843741799845 1101 6 0.05778561703570517 1101 7 0.12160501127829804 1101 8 0.1538989638914643 1101 9 0.21972766034852498 1101 10-11 0.16960475182566626 1101 12-13 0.02272145488002053 1101 14-15 0.048419939573527415 1101 16-17 0.10819528203509776 1101 18-19 0.13293964883435905 1101 20-21 0.11365394015892605 1101 22-23 -0.10299001547453912 1101 24-25 -0.01901761712914407 1101 26-27 0.16817109947775322 1101 28-29 0.15311927440065887 1101 30-31 0.19342214529049784 1101 32-33 0.2710147242531846 1101 34-35 0.3803409717614912 1101 36-37 0.4088875652414714 1101 38-39 0.5659018764091215 1101 40-41 0.4563086193164807 1101 42-43 0.5840129587265892 1101 44-45 0.5830279837859536 1101 46-47 0.7756856714964329 1101 48-49 0.58477635148855 1101 50-51 0.7488252526024226 1101 52-53 0.6549847795238293 1101 54-55 0.6767970523037121 1101 56-57 0.7221022269585617 1101 58-59 0.6528830128720315 1101 60-61 0.7281289735556271 1101 62-63 0.8453268259337605 1101 64-65 0.5436313298856241 1101 66-67 0.44164606256380523 1101 68-69 0.6720313423951723 1101 70-71 0.5383844374224473 1101 72-73 0.7863466430631405 1101 74-75 0.6749275190628126 1101 76-77 0.5410459631402098 1101 78-79 0.36537566802948973 1101 80-81 0.6936001802770804 1101 82-83 0.7567414457120982 1101 84-85 0.5419238012795908 1101 86-87 0.35265309146339163 1101 88-89 0.24186503826487282 1101 90-91 0.4533138626511004 1101 92-93 0.5405751091141582 1101 94-95 0.4034408722101368 1101 96-97 0.17878472528817113 1101 98-99 0.4647325961077584 1101 100-101 0.35442611137004576 1102 1 0.13923178976968842 1102 2 0.008765823411565066 1102 3 0.07944550134476458 1102 4 0.1578985046629171 1102 5 0.1974720945261268 1102 6 0.21376833063973777 1102 7 0.206406003029862 1102 8 0.22440197308178966 1102 9 0.38658016500602344 1102 10-11 0.28031456060876536 1102 12-13 0.330349700407794 1102 14-15 0.37500539157537816 1102 16-17 0.27767087261900514 1102 18-19 0.2779382983346679 1102 20-21 0.18411539551279077 1102 22-23 -0.028013043225577405 1102 24-25 0.11122595760890164 1102 26-27 0.15703293989717082 1102 28-29 0.12965174526103596 1102 30-31 -0.1999472475386881 1102 32-33 -0.2726306553064788 1102 34-35 -0.024009022351712872 1102 36-37 -0.002453861855784112 1102 38-39 0.1534263758585368 1102 40-41 0.03607813403693427 1102 42-43 -0.0014719399166764902 1102 44-45 0.05529426076997623 1102 46-47 -0.13719151613991443 1102 48-49 0.08349337678791358 1102 50-51 0.13135082552594923 1102 52-53 0.2964877125321266 1102 54-55 0.2330470955889581 1102 56-57 0.33360848427376055 1102 58-59 -0.0021733964609538248 1102 60-61 0.16959000644548894 1102 62-63 -0.00748775392232659 1102 64-65 -0.011964240475403898 1102 66-67 0.2899918735908109 1102 68-69 0.24502238754341477 1102 70-71 -0.08854002462851085 1102 72-73 0.168858364707841 1102 74-75 0.09262495172828267 1102 76-77 0.16580616287565064 1102 78-79 0.11160292596107624 1102 80-81 0.10325348276823121 1102 82-83 0.16557579134306266 1102 84-85 0.11527497967713529 1102 86-87 0.15040018096342678 1102 88-89 0.21695005048862726 1102 90-91 0.2940362760979234 1102 92-93 0.4664344078239111 1102 94-95 0.5058347194720874 1102 96-97 0.5982793613804489 1102 98-99 0.6871287288307144 1102 100-101 0.8262820442190488 1103 1 0.07285850787916104 1103 2 -0.20992167570189935 1103 3 0.039026488309119145 1103 4 0.08957129434787703 1103 5 0.013969491652325416 1103 6 0.2501872869369919 1103 7 0.1395327140470357 1103 8 0.24044829998003792 1103 9 0.21800330575978677 1103 10-11 0.27149351350171713 1103 12-13 0.22349357478898924 1103 14-15 0.32030248798809424 1103 16-17 0.21371251859979878 1103 18-19 0.2606047191349674 1103 20-21 0.22086237064719683 1103 22-23 0.30716901609441294 1103 24-25 0.27818222649214164 1103 26-27 0.08816139561994163 1103 28-29 0.07084417352243122 1103 30-31 0.015747301320161 1103 32-33 -0.08508544189919576 1103 34-35 -0.3339903696048694 1103 36-37 -0.35545354979461763 1103 38-39 -0.33685185503835413 1103 40-41 -0.2626495074898969 1103 42-43 -0.3517269080722514 1103 44-45 0.039088357140137475 1103 46-47 -0.04345082478258888 1103 48-49 -0.2450378789745784 1103 50-51 -0.02873300545038404 1103 52-53 -0.07056359316015914 1103 54-55 0.03190239850105314 1103 56-57 0.17880487003809975 1103 58-59 0.12665467202228342 1103 60-61 0.1416215246230479 1103 62-63 0.12502831013531335 1103 64-65 0.14005032710711518 1103 66-67 -0.0316438942924222 1103 68-69 0.2874095136019399 1103 70-71 0.48651068531063757 1103 72-73 0.5234733237852716 1103 74-75 0.789453984764215 1103 76-77 0.5983371207615811 1103 78-79 0.5658877385116092 1103 80-81 0.576712326156084 1103 82-83 0.8187623613653265 1103 84-85 0.9091834039101059 1103 86-87 0.5040594527261497 1103 88-89 0.6099414404374208 1103 90-91 0.9093393300419486 1103 92-93 0.9916404391151374 1103 94-95 0.7972342428695818 1103 96-97 0.8949591015186051 1103 98-99 0.9661096363613169 1103 100-101 1.0635425018141902 1104 1 0.16229701055296175 1104 2 0.057591693282056156 1104 3 0.17178049900430636 1104 4 0.13288680236927064 1104 5 -0.04351713936371482 1104 6 0.0642247200920707 1104 7 0.12268779426094056 1104 8 0.1925873374131939 1104 9 0.27014234319294417 1104 10-11 0.17069137446427618 1104 12-13 0.1595898314734825 1104 14-15 0.20566345055494395 1104 16-17 0.1621750854447086 1104 18-19 0.18787744640768977 1104 20-21 0.08657092679692369 1104 22-23 0.2107117968430785 1104 24-25 -0.004371249443686054 1104 26-27 0.08762663626165335 1104 28-29 0.1276623553406111 1104 30-31 0.13145585746988786 1104 32-33 0.3320268575660421 1104 34-35 0.0833224646197337 1104 36-37 0.16954645020538095 1104 38-39 0.05713210218089415 1104 40-41 0.23701626791117292 1104 42-43 0.11438271759620022 1104 44-45 0.2665616191722222 1104 46-47 0.3595571966077884 1104 48-49 -0.4022571303114759 1104 50-51 -0.3311394225626785 1104 52-53 -0.1851357856735234 1104 54-55 -0.025717922354559164 1104 56-57 0.14544925506483963 1104 58-59 -0.05489613011675232 1104 60-61 0.01368034815246233 1104 62-63 -0.004049229971634816 1104 64-65 -0.031005822625505886 1104 66-67 0.28700583832790016 1104 68-69 0.36468224087466794 1104 70-71 0.25268715589887236 1104 72-73 0.3795562114858093 1104 74-75 0.024948637170631116 1104 76-77 0.1276820405476755 1104 78-79 -0.04487429357395101 1104 80-81 -0.08892938507386461 1104 82-83 0.08473829719420678 1104 84-85 0.10483848412400931 1104 86-87 0.005730575720800601 1104 88-89 0.016425397656671947 1104 90-91 0.14630457068366098 1104 92-93 0.2522687813611242 1104 94-95 0.25498825356477184 1104 96-97 0.41367567905871283 1104 98-99 0.4401069625645242 1104 100-101 0.4850665659853135 1105 1 0.3027415650975982 1105 2 0.15746332646826033 1105 3 0.12039098454614816 1105 4 0.04457944267194591 1105 5 0.15988795927604826 1105 6 0.13091465793551293 1105 7 0.1045317722809429 1105 8 0.2134953637171293 1105 9 0.1881001848288335 1105 10-11 0.31555895396162015 1105 12-13 0.18786205920071097 1105 14-15 0.41170907707507354 1105 16-17 0.3954795174778596 1105 18-19 0.3647128666401045 1105 20-21 0.3806851957817088 1105 22-23 0.37253167793486597 1105 24-25 0.4456343806797278 1105 26-27 0.44146187633011635 1105 28-29 0.20844336606037217 1105 30-31 0.3729960340305638 1105 32-33 0.584098742981773 1105 34-35 0.5814093486842324 1105 36-37 0.404151440746773 1105 38-39 0.6059430201162215 1105 40-41 0.4160485622990606 1105 42-43 0.2838618185780888 1105 44-45 0.5301989041015034 1105 46-47 0.665366357944599 1105 48-49 0.7279856406100578 1105 50-51 0.6966868993902935 1105 52-53 0.7390624847525658 1105 54-55 0.7575290824197793 1105 56-57 0.9522650824677825 1105 58-59 0.8236823763241077 1105 60-61 0.7822845279560795 1105 62-63 0.7871328707400167 1105 64-65 1.0297398309192225 1105 66-67 0.8954183687305601 1105 68-69 0.9560388754120979 1105 70-71 0.7719579423145113 1105 72-73 0.7828070447440716 1105 74-75 0.780996515740128 1105 76-77 0.7454000389261992 1105 78-79 0.8764852686276505 1105 80-81 1.2594221147910112 1105 82-83 1.2255398015631833 1105 84-85 1.2683361419926662 1105 86-87 1.1872176039164586 1105 88-89 1.238316443508399 1105 90-91 1.4481748976812305 1105 92-93 1.7355169121601506 1105 94-95 1.7884154222883062 1105 96-97 2.1504899300270957 1105 98-99 2.0318070346301056 1105 100-101 1.975734073417076 1106 1 0.27621301363541306 1106 2 0.20703299818829635 1106 3 0.21514679748876375 1106 4 0.14254516353032898 1106 5 0.270636469678152 1106 6 0.22084557804223692 1106 7 0.32711882431488704 1106 8 0.22541549050849596 1106 9 0.3479101315309876 1106 10-11 0.286482538091299 1106 12-13 0.34133912507738273 1106 14-15 0.6345903706679579 1106 16-17 0.4520190361254208 1106 18-19 0.44286074977271284 1106 20-21 0.2794844896019626 1106 22-23 0.33624801569742857 1106 24-25 0.3897752956372287 1106 26-27 0.42715270869936717 1106 28-29 0.6844309142925766 1106 30-31 0.7223594022939324 1106 32-33 0.8194401634997703 1106 34-35 0.438745660098796 1106 36-37 0.6074606551886887 1106 38-39 0.5356961915721072 1106 40-41 0.6617369203642909 1106 42-43 0.8306779911641371 1106 44-45 0.820899533377208 1106 46-47 0.6557266032350597 1106 48-49 0.6200842516561593 1106 50-51 0.5407973870956795 1106 52-53 0.6043132253719392 1106 54-55 0.6122155479768026 1106 56-57 0.7620078217229462 1106 58-59 0.6148444298626927 1106 60-61 0.7578557912554658 1106 62-63 0.8262519516363795 1106 64-65 0.7821556209912437 1106 66-67 0.6914721881866228 1106 68-69 0.8606878920434333 1106 70-71 0.8102327505559508 1106 72-73 0.6734394634765621 1106 74-75 0.7529315552286846 1106 76-77 0.7254183621505526 1106 78-79 0.6833976047050108 1106 80-81 0.33526789209027896 1106 82-83 0.4813443850441921 1106 84-85 0.5270931720253671 1106 86-87 0.6329300362910537 1106 88-89 0.8033538589975429 1106 90-91 0.7452802963451042 1106 92-93 0.8855817790492821 1106 94-95 1.1492792887561372 1106 96-97 1.3501963571989641 1106 98-99 1.216824789433261 1106 100-101 1.5334739638789685 1107 1 0.08586904806361417 1107 2 0.10514071288989868 1107 3 0.1745085467451375 1107 4 0.1108066659668836 1107 5 0.24623136873517382 1107 6 0.14636453253879012 1107 7 0.13023255129419198 1107 8 0.14787292991106682 1107 9 0.17061122639839965 1107 10-11 0.15446162169360633 1107 12-13 0.07565973769683865 1107 14-15 0.28249635763081216 1107 16-17 0.26835581860753877 1107 18-19 0.26041580957905097 1107 20-21 0.13288379977220188 1107 22-23 0.016572836911279865 1107 24-25 0.21129371219314663 1107 26-27 0.2303376337041172 1107 28-29 0.14668409447104835 1107 30-31 0.26520479182624257 1107 32-33 0.4907544790494214 1107 34-35 0.5161656018746399 1107 36-37 0.5628115823451907 1107 38-39 0.44959800158413543 1107 40-41 0.8200299081498699 1107 42-43 0.71980684376841 1107 44-45 0.9451954640145104 1107 46-47 1.1502008453716357 1107 48-49 1.3248102183671193 1107 50-51 1.2759684205745785 1107 52-53 1.485804112024688 1107 54-55 1.2746657093589988 1107 56-57 1.4268431169574214 1107 58-59 1.2769346456718225 1107 60-61 1.1925592910339589 1107 62-63 1.2375748109490843 1107 64-65 1.1836254646720228 1107 66-67 0.7251132552076953 1107 68-69 0.8620287881892459 1107 70-71 0.9609352377403049 1107 72-73 0.7509543359529864 1107 74-75 1.0156562245534104 1107 76-77 1.1910771812126377 1107 78-79 1.1576832511830837 1107 80-81 1.3386814418656066 1107 82-83 1.4641500619000887 1107 84-85 1.4746914253004775 1107 86-87 1.3135950258486773 1107 88-89 1.463153871654967 1107 90-91 1.4377048264381358 1107 92-93 1.3983578691701624 1107 94-95 1.3673390634539402 1107 96-97 1.419270308214724 1107 98-99 1.411132111754636 1107 100-101 1.6065073161984422 1108 1 0.0934286157374089 1108 2 -0.1327123944547317 1108 3 -0.01837403738652199 1108 4 0.1212217973842229 1108 5 0.014807885561509693 1108 6 -0.08973839057592414 1108 7 -0.009266343326295612 1108 8 0.11661525366444181 1108 9 0.2618173182677168 1108 10-11 0.13282995871153958 1108 12-13 0.12010827613948294 1108 14-15 0.22260014048514876 1108 16-17 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sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 27.0 6 49.0 7 87.0 8 260.0 9 508.0 10 989.0 11 1581.0 12 2680.0 13 3533.0 14 4455.0 15 6200.0 16 8640.0 17 12722.0 18 10688.0 19 5185.0 20 3651.0 21 3387.0 22 3424.0 23 3529.0 24 4017.0 25 4779.0 26 5494.0 27 6705.0 28 8190.0 29 10014.0 30 12624.0 31 15560.0 32 19723.0 33 25761.0 34 35647.0 35 50367.0 36 76005.0 37 116828.0 38 88079.0 39 6446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0224835945064 20.721662423351383 15.349625273424895 19.906228708717325 2 15.951935349711025 22.228914798720762 39.744044802019246 22.075105049548966 3 18.695480702005987 27.239481562483192 32.65349658498109 21.411541150529732 4 12.282981831870861 19.10369367835151 38.78407084405167 29.829253645725963 5 11.86783401811259 40.05259534923742 33.62212374785783 14.457446884792164 6 27.497472411641965 39.2107829429016 18.30314568230545 14.98859896315099 7 25.55530658157732 37.43566761376368 20.16635445329796 16.84267135136104 8 27.131909401357007 33.253681578216174 19.78757540176931 19.826833618657513 9 28.140433274475885 15.680341313447283 21.04867839632873 35.13054701574811 10-11 24.32568574217367 27.848889789179605 27.065696623342166 20.75972784530456 12-13 26.240117022697344 26.81106875531626 27.62268067664503 19.326133545341364 14-15 23.130887612150328 26.732067151269618 28.59306796690837 21.543977269671682 16-17 20.104330055839885 29.89369807025249 28.457098297914296 21.544873575993332 18-19 20.005646729826385 27.305792827756814 33.417705635077844 19.270854807338957 20-21 21.649407839836655 26.24444163594497 33.51985952722198 18.5862909969964 22-23 21.1520225152148 26.296014125787632 33.22042861368301 19.331534745314556 24-25 20.46121908982371 26.578269926464525 33.75281897939501 19.20769200431676 26-27 20.384504033895297 26.66090630660831 34.0826283017126 18.871961357783793 28-29 19.913037320810897 27.26470912498622 33.756434583933505 19.065818970269376 30-31 20.595891847700393 26.646066740890944 33.82448648096255 18.933554930446117 32-33 20.2541584429972 26.719710455129686 33.68119231107072 19.344938790802395 34-35 20.357046501017788 26.894204560020007 33.562138048299964 19.18661089066224 36-37 21.0655812644422 26.3130863152913 33.308534724923675 19.31279769534282 38-39 20.2545203900447 27.426194375203355 32.926581139354305 19.392704095397644 40-41 20.36036617448413 27.500284589245844 32.92253884082991 19.216810395440117 42-43 20.454111554191954 27.518457469168833 32.2624102223501 19.765020754289115 44-45 21.00521204461833 27.526228281280112 31.551468380412036 19.917091293689527 46-47 21.262306307856676 27.7367866285198 30.742107112382673 20.25879995124085 48-49 21.249149627090752 28.28113978203564 30.176653487940612 20.29305710293299 50-51 21.351540302413753 28.826286871801305 29.355555754734734 20.4666170710502 52-53 21.612803763113174 28.917997404255015 28.84216866605956 20.627030166572254 54-55 21.568001778316372 28.53292484641406 28.805050275083318 21.094023100186256 56-57 21.790181108304665 29.24908979766709 28.226042094281006 20.73468699974724 58-59 21.540291411812788 29.1441391376677 28.272287912576456 21.043281537943052 60-61 21.749204291938586 28.83066991851555 28.786212447554078 20.633913341991782 62-63 21.57059756742464 28.41595783775063 28.753058645322625 21.2603859495021 64-65 21.450748687354686 28.431207346869996 29.177743558671526 20.94030040710379 66-67 21.455889589303112 29.015343881673118 28.347326181400014 21.181440347623756 68-69 21.54523209852199 28.547804497665126 28.62802391345266 21.278939490360223 70-71 21.079888750359643 28.982001256622592 29.05388508804873 20.884224904969038 72-73 21.56310724862148 28.643491728870433 28.862639017359694 20.93076200514839 74-75 21.042569325060995 28.91431818629973 28.413998203795693 21.629114284843585 76-77 20.680101749058426 29.09178747425351 28.643094920972388 21.585015855715675 78-79 20.73014971017909 29.213517387013766 28.139831081959223 21.916501820847927 80-81 20.594132710912003 28.724063163574726 28.40935675659687 22.2724473689164 82-83 20.482127491753907 29.486591137243657 28.358400258138534 21.672881112863905 84-85 20.563056340590915 28.95297048117663 28.01908465026563 22.464888527966828 86-87 20.260487111436174 28.913244759469347 27.938681287359536 22.887586841734944 88-89 20.443478019254606 28.782689675633087 28.10274621509195 22.671086090020356 90-91 20.21959504880388 28.6391381118411 28.434152856080093 22.707113983274922 92-93 20.09502648139401 28.549533068803225 28.552759774453275 22.802680675349492 94-95 19.974544900465183 29.121888696680976 28.22190751911373 22.68165888374011 96-97 20.601355766309126 28.922745700152102 27.48469765945223 22.99120087408654 98-99 19.861051605050257 28.9041702444997 27.586661240909017 23.648116909541027 100-101 19.939606555261165 29.14436345775327 27.468171991352392 23.44785799563317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 504.0 1 343.5 2 186.0 3 182.0 4 170.0 5 179.0 6 265.5 7 480.5 8 742.0 9 905.0 10 864.0 11 781.5 12 804.5 13 905.0 14 1053.5 15 1142.0 16 1249.5 17 1385.0 18 1536.5 19 1707.0 20 1955.0 21 2267.0 22 2587.0 23 2935.0 24 3257.0 25 4150.0 26 5855.0 27 7934.5 28 8679.0 29 9116.0 30 10549.5 31 11957.0 32 13641.0 33 15295.0 34 17890.5 35 19461.0 36 20312.5 37 20383.5 38 17601.5 39 15602.0 40 15403.0 41 15562.5 42 15632.5 43 16466.5 44 17312.5 45 16697.0 46 16100.0 47 16502.5 48 17967.0 49 18127.5 50 16743.5 51 15651.5 52 14783.0 53 14136.5 54 13074.5 55 12281.5 56 11664.0 57 10874.0 58 10355.0 59 9696.5 60 8504.5 61 6980.5 62 5585.5 63 4121.0 64 2794.5 65 2083.0 66 1598.0 67 1162.0 68 841.5 69 598.0 70 460.0 71 371.5 72 285.0 73 249.5 74 186.5 75 149.0 76 119.5 77 60.5 78 35.5 79 20.5 80 8.0 81 7.5 82 10.0 83 6.5 84 6.0 85 5.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01882243275461822 2 0.002330396436286065 3 0.0026889189649454597 4 0.0 5 1.7926126432969732E-4 6 1.7926126432969732E-4 7 0.0 8 0.0 9 0.0 10-11 8.963063216484866E-5 12-13 8.06675689483638E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002599288332780611 22-23 0.0 24-25 0.003405964022264249 26-27 0.0017029820111321245 28-29 0.003495594654429098 30-31 0.013623856089056996 32-33 7.170450573187893E-4 34-35 0.0033163333900994006 36-37 0.003226702757934552 38-39 0.0020615045397915192 40-41 0.004212639711747887 42-43 0.001523720746802427 44-45 4.481531608242433E-4 46-47 1.7926126432969732E-4 48-49 2.6889189649454597E-4 50-51 0.0 52-53 0.001613351378967276 54-55 0.0025096577006157627 56-57 3.5852252865939464E-4 58-59 0.001613351378967276 60-61 0.001523720746802427 62-63 0.0 64-65 3.5852252865939464E-4 66-67 8.963063216484866E-5 68-69 0.0 70-71 8.963063216484866E-5 72-73 1.7926126432969732E-4 74-75 3.5852252865939464E-4 76-77 3.5852252865939464E-4 78-79 8.963063216484866E-5 80-81 0.004212639711747887 82-83 8.963063216484866E-4 84-85 0.002599288332780611 86-87 9.859369538133354E-4 88-89 8.06675689483638E-4 90-91 0.0 92-93 8.963063216484866E-5 94-95 0.0 96-97 9.859369538133354E-4 98-99 0.0021511351719563677 100-101 5.377837929890919E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557845.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91226645861139 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25844828577331 42.73002583078682 2 12.112613903235795 13.060369365230585 3 3.7807492241605574 6.114862787583968 4 1.6553671964081886 3.569783895184111 5 0.8318241865592959 2.2422763596251216 6 0.46447353918832696 1.502449272461722 7 0.31098612948715426 1.173617695449058 8 0.22716998060346638 0.979779882055333 9 0.15630686299227878 0.7584171522254457 >10 0.9883836366963038 10.537117802700065 >50 0.12267263138420494 4.593009289709061 >100 0.08567083092438536 8.801066175843287 >500 0.004000194440049118 1.3669221051511888 >1k 0.0010000486100122795 1.5464586793092268 >5k 3.3334953667075984E-4 1.0238437066850279 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5697 1.0212514228862857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4637 0.8312344826968064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2893 0.5186028377058143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1075 0.19270585915442462 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 937 0.16796780467692637 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 776 0.1391067411198451 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 675 0.12100135342254568 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.11508573169966567 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 626 0.11221755147039052 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.11024567756276385 No Hit ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT 590 0.10576414595452141 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 574 0.10289596572524626 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 567 0.10164113687493838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7926126432969732E-4 0.0 18-19 0.0 0.0 0.0 7.170450573187893E-4 0.0 20-21 0.0 0.0 0.0 8.963063216484866E-4 0.0 22-23 0.0 0.0 0.0 0.0012548288503078811 0.0 24-25 0.0 0.0 0.0 0.0021511351719563677 0.0 26-27 0.0 0.0 0.0 0.0037644865509236436 0.0 28-29 0.0 0.0 0.0 0.006274144251539406 0.0 30-31 0.0 0.0 0.0 0.011831243445760024 0.0 32-33 0.0 0.0 0.0 0.017746865168640033 0.0 34-35 0.0 0.0 0.0 0.02330396436286065 0.0 36-37 0.0 0.0 0.0 0.029847000510894603 0.0 38-39 0.0 0.0 0.0 0.035941883498104316 0.0 40-41 0.0 0.0 0.0 0.046518298093556454 0.0 42-43 0.0 0.0 0.0 0.06605777590549347 0.0 44-45 0.0 0.0 0.0 0.09303659618711291 0.0 46-47 0.0 0.0 0.0 0.11929837141141357 0.0 48-49 0.0 0.0 0.0 0.16734039025177244 0.0 50-51 0.0 0.0 0.0 0.2245247335729459 0.0 52-53 0.0 0.0 0.0 0.2875350679848345 0.0 54-55 0.0 0.0 0.0 0.4057578718102699 0.0 56-57 0.0 0.0 0.0 0.5151968736835502 0.0 58-59 0.0 0.0 0.0 0.6650592906631771 0.0 60-61 0.0 0.0 0.0 0.8280077799388719 0.0 62-63 0.0 0.0 0.0 1.0433901890310033 0.0 64-65 0.0 0.0 0.0 1.2092068585359732 0.0 66-67 0.0 0.0 0.0 1.4293396911328415 0.0 68-69 0.0 0.0 0.0 1.7218044438867428 0.0 70-71 0.0 0.0 0.0 1.9024101676989127 0.0 72-73 0.0 0.0 0.0 2.1831333076392188 0.0 74-75 0.0 0.0 0.0 2.538518764172844 0.0 76-77 0.0 0.0 0.0 2.8007779938871913 0.0 78-79 0.0 0.0 0.0 3.0270953401034335 0.0 80-81 0.0 0.0 0.0 3.3823015353727293 0.0 82-83 0.0 0.0 0.0 3.8274072547033673 0.0 84-85 0.0 0.0 0.0 4.35595909257948 0.0 86-87 0.0 0.0 0.0 5.09666663678979 0.0 88-89 0.0 0.0 0.0 5.864621893178213 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3105 0.0 47.730186 1 GTATCAA 5240 0.0 40.067398 1 CCGACCA 70 2.0528812E-4 33.92604 9 TACACGG 85 1.630534E-5 33.52992 2 GTACACG 90 2.4105046E-5 31.667145 1 GATAGTC 75 3.077777E-4 31.664307 7 TATCAAC 6835 0.0 30.578367 2 ATCAACG 6890 0.0 30.400494 3 TCAACGC 6915 0.0 30.359268 4 CAACGCA 7060 0.0 29.735743 5 TACGACG 65 0.005843655 29.22859 5 AACGCAG 7210 0.0 29.051233 6 CGGTGTA 90 8.9456455E-4 26.38692 9 ACGCAGA 8160 0.0 25.610836 7 CGCAGAG 8165 0.0 25.47881 8 GTACATG 4460 0.0 24.495886 1 TACATGG 4375 0.0 24.320368 2 GCAGAGT 8510 0.0 23.943573 9 CGTAACG 50 0.0016534697 23.75036 78-79 GATCGTC 100 0.0016532993 23.74823 7 >>END_MODULE