FastQCFastQC Report
Thu 26 May 2016
SRR937038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428263
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59611.3919017052605525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44081.0292740675706282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.6601083913389669No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.22976535446676458No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA6110.1426693410357654No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5840.13636480387051883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.1344967928585939No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5560.1298267653287816No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5240.12235472128108195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5060.11815169650425089No Hit
GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC5000.1167506882453072No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4840.11301466622145738No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4330.10110609602043605No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA4290.10017209051447358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.049.064751
GTATCAA41200.043.239221
TATCAAC52450.033.9688072
TATAGAG851.6272295E-533.5372055
ATCAACG53550.033.1823123
TCAACGC53700.033.0896264
CAACGCA54250.032.7541545
AACGCAG55300.032.0425836
TACTAGG600.003943716631.6740282
CGTCACT902.4075493E-531.6703281
ACGCAGA60300.029.2280887
CGCAGAG60600.028.9299898
GCAGAGT62800.027.3869389
TAGATTG700.00837628927.1491665
TGACGAG350.00839283627.1412460-61
GAGTACT40900.025.32373412-13
GTACATG26700.025.265091
TACATGG26250.025.1582282
CGGGACT950.00122363825.005814
TCACTAC1351.3377576E-524.6353533
Produced by FastQC (version 0.11.5)