##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937038_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 428263 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.043260800022416 33.0 31.0 34.0 30.0 34.0 2 32.145952370389224 33.0 31.0 34.0 30.0 34.0 3 32.11969514060285 34.0 31.0 34.0 30.0 34.0 4 35.74008027777324 37.0 35.0 37.0 35.0 37.0 5 35.76122849744199 37.0 35.0 37.0 35.0 37.0 6 35.85495594996532 37.0 35.0 37.0 35.0 37.0 7 35.81660801890427 37.0 35.0 37.0 35.0 37.0 8 35.777083240905704 37.0 35.0 37.0 35.0 37.0 9 37.4369838160196 39.0 37.0 39.0 35.0 39.0 10-11 37.28388747101664 39.0 37.0 39.0 34.0 39.0 12-13 37.23173377107058 39.0 37.0 39.0 34.0 39.0 14-15 38.34910090294983 40.0 38.0 41.0 34.0 41.0 16-17 38.321705353953064 40.0 38.0 41.0 34.0 41.0 18-19 38.421828409178474 40.0 38.0 41.0 34.0 41.0 20-21 38.408884260372716 40.0 38.0 41.0 34.0 41.0 22-23 38.293519402796875 40.0 38.0 41.0 34.0 41.0 24-25 38.15163579389301 40.0 38.0 41.0 34.0 41.0 26-27 38.13629825597822 40.0 38.0 41.0 34.0 41.0 28-29 38.02469860809829 40.0 38.0 41.0 33.5 41.0 30-31 37.741115856377974 40.0 37.0 41.0 33.0 41.0 32-33 37.37034952821047 40.0 35.5 41.0 33.0 41.0 34-35 37.107010178325 39.5 35.0 41.0 32.5 41.0 36-37 36.862670835444575 39.0 35.0 41.0 31.5 41.0 38-39 36.59959534211454 39.0 35.0 41.0 31.0 41.0 40-41 36.331186677345464 39.0 35.0 41.0 30.5 41.0 42-43 35.939346849949686 39.0 35.0 41.0 30.0 41.0 44-45 35.355264171782295 39.0 35.0 41.0 27.0 41.0 46-47 34.56282121033104 39.0 35.0 40.5 21.5 41.0 48-49 33.88848674762937 38.0 34.0 40.0 11.0 41.0 50-51 33.06488536249921 38.0 33.5 39.5 3.5 40.5 52-53 33.21958352694489 38.0 33.5 40.0 2.0 40.5 54-55 33.52621636704548 39.0 34.0 40.5 2.0 41.0 56-57 33.39613625272321 39.0 34.0 41.0 2.0 41.0 58-59 33.15752936863563 38.0 34.0 40.5 2.0 41.0 60-61 32.89480295986345 38.0 34.0 40.0 2.0 41.0 62-63 32.598551590961634 37.5 33.0 40.0 2.0 41.0 64-65 32.27921394096619 37.0 33.0 40.0 2.0 41.0 66-67 31.916267573897347 36.5 32.5 39.5 2.0 41.0 68-69 31.52166075519015 36.0 32.0 39.0 2.0 41.0 70-71 31.142026044743535 35.5 31.5 39.0 2.0 41.0 72-73 30.768035062566696 35.0 31.0 38.5 2.0 40.0 74-75 30.35948354165548 35.0 31.0 37.5 2.0 40.0 76-77 29.963779266478774 35.0 31.0 37.0 2.0 39.0 78-79 29.54636987085039 35.0 30.0 36.5 2.0 39.0 80-81 29.141856756245577 35.0 30.0 36.0 2.0 38.5 82-83 28.75750648550073 35.0 29.0 35.5 2.0 37.0 84-85 28.446326206092984 34.0 29.0 35.0 2.0 37.0 86-87 28.137363022255016 34.0 29.0 35.0 2.0 36.0 88-89 27.799442165211566 34.0 28.5 35.0 2.0 36.0 90-91 27.48055050284521 34.0 27.0 35.0 2.0 36.0 92-93 27.169553522018013 34.0 26.5 35.0 2.0 35.0 94-95 26.79800379673238 34.0 25.5 35.0 2.0 35.0 96-97 26.41123211671333 34.0 25.0 35.0 2.0 35.0 98-99 25.937160109558846 34.0 23.5 35.0 2.0 35.0 100-101 24.109916102955427 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28176114159248655 1101 2 0.19473002201125666 1101 3 0.19025908586324647 1101 4 0.15727119913425724 1101 5 0.13429831710405438 1101 6 -0.030411932594375912 1101 7 0.13054006088439962 1101 8 0.13210808093386106 1101 9 0.03721461811386462 1101 10-11 0.039386386330853895 1101 12-13 0.09647165923492906 1101 14-15 0.4334507271993644 1101 16-17 0.3899486654598121 1101 18-19 0.27716930604754 1101 20-21 0.4108257865674929 1101 22-23 0.476886643822624 1101 24-25 0.2947648052967722 1101 26-27 0.34262589552422895 1101 28-29 0.4146134802773602 1101 30-31 0.522378441960953 1101 32-33 0.45575739050508446 1101 34-35 0.3341758238187609 1101 36-37 0.3036950884160845 1101 38-39 0.17803972976995652 1101 40-41 0.2878399037899442 1101 42-43 0.4222257523692008 1101 44-45 0.7424683202695306 1101 46-47 0.9126550829071576 1101 48-49 0.959649444724711 1101 50-51 1.2176650613739426 1101 52-53 1.2154129967518728 1101 54-55 1.1320391204430962 1101 56-57 1.2810253060806147 1101 58-59 1.2313407891048982 1101 60-61 1.166857505203616 1101 62-63 1.020890838458918 1101 64-65 1.2118461679301085 1101 66-67 1.3654792436969494 1101 68-69 1.3466011858617861 1101 70-71 1.3415924907584547 1101 72-73 1.4446831243287086 1101 74-75 1.4204676256638002 1101 76-77 1.4193777648164456 1101 78-79 1.2512655051001573 1101 80-81 1.2967376199648477 1101 82-83 1.3861271298669209 1101 84-85 1.4539692394954855 1101 86-87 1.397983665604709 1101 88-89 1.4858380072936832 1101 90-91 1.448476568849582 1101 92-93 1.4648573874359023 1101 94-95 1.61243238128381 1101 96-97 1.5278282068589313 1101 98-99 1.2679327783906515 1101 100-101 1.2342210436505567 1102 1 0.1499047524730237 1102 2 0.20451143808399763 1102 3 0.15283362479522822 1102 4 0.03605873097728818 1102 5 0.15658032948824996 1102 6 -0.049289722139363334 1102 7 0.03141496905243457 1102 8 0.05663317849041505 1102 9 -0.1974725807488511 1102 10-11 0.16684151985691642 1102 12-13 0.16697761757900054 1102 14-15 0.07583845367845754 1102 16-17 0.15004827332540316 1102 18-19 0.056765913273878255 1102 20-21 0.11186766194316533 1102 22-23 0.0918583548071581 1102 24-25 0.11846365370008982 1102 26-27 0.21630709310845475 1102 28-29 0.09656480218059471 1102 30-31 0.2925056232682621 1102 32-33 0.3420288696437268 1102 34-35 0.4268531191408229 1102 36-37 0.2947084362517174 1102 38-39 0.4711072513087231 1102 40-41 0.7365008267846704 1102 42-43 0.5929120352975161 1102 44-45 0.6317032288191982 1102 46-47 0.8770023365351136 1102 48-49 1.2931430665773789 1102 50-51 1.4181506974235916 1102 52-53 1.4555585921569332 1102 54-55 1.4795894729824326 1102 56-57 1.384090824012894 1102 58-59 1.2789798727888666 1102 60-61 1.2222133361755638 1102 62-63 1.2918862492459482 1102 64-65 1.3679576447789081 1102 66-67 1.2974739770741728 1102 68-69 1.2003140595105393 1102 70-71 1.3783548534875543 1102 72-73 1.4982948635545554 1102 74-75 1.3751336435436023 1102 76-77 1.3371154068578335 1102 78-79 1.4103432736549806 1102 80-81 1.4204316978765164 1102 82-83 1.264763914173912 1102 84-85 1.2394745776756402 1102 86-87 1.2762645712348437 1102 88-89 1.2891992957080696 1102 90-91 1.2409859428656667 1102 92-93 1.4770240493320799 1102 94-95 1.2735710022300815 1102 96-97 1.2129270991507717 1102 98-99 1.0395149596502549 1102 100-101 0.866024003263572 1103 1 0.3283514850469409 1103 2 0.21756061766414803 1103 3 0.3698509225979407 1103 4 0.3146297523116246 1103 5 0.256282720621563 1103 6 0.18206525340197288 1103 7 0.15469801674966988 1103 8 -0.016423526831196966 1103 9 0.0026368845814275232 1103 10-11 0.2723546956057845 1103 12-13 0.2740392716457549 1103 14-15 0.4401538962718732 1103 16-17 0.38108853604219206 1103 18-19 0.324788353666591 1103 20-21 0.42975726159652794 1103 22-23 0.5442169905954728 1103 24-25 0.4492861260201835 1103 26-27 0.3218693002031756 1103 28-29 0.3689191049662597 1103 30-31 0.40508957519472943 1103 32-33 0.4911149481622985 1103 34-35 0.6628136204008186 1103 36-37 0.43696205373879593 1103 38-39 0.7339050027084042 1103 40-41 0.9860977435111948 1103 42-43 0.9484577085663162 1103 44-45 1.1208821248157363 1103 46-47 1.340264175324613 1103 48-49 1.3217864946666396 1103 50-51 1.4119408204580637 1103 52-53 1.3737172940805564 1103 54-55 1.5439687703518388 1103 56-57 1.7327093943500742 1103 58-59 1.7988204639016487 1103 60-61 1.8125518805147252 1103 62-63 1.5520340274418274 1103 64-65 1.6533759505748726 1103 66-67 1.5575316744253378 1103 68-69 1.720634701707148 1103 70-71 1.6208524874400503 1103 72-73 1.4936129401574796 1103 74-75 1.695280965633934 1103 76-77 1.7565272587603644 1103 78-79 1.6238223327092491 1103 80-81 1.6954268517551263 1103 82-83 1.77512663210684 1103 84-85 1.7698477860360526 1103 86-87 1.78125892692875 1103 88-89 1.8535831541329095 1103 90-91 1.8110118268552178 1103 92-93 1.9004804171355865 1103 94-95 2.040223985730467 1103 96-97 2.272991638085081 1103 98-99 2.383496076880782 1103 100-101 2.42653894642142 1104 1 0.32914688289261207 1104 2 0.26941997019269337 1104 3 0.3923539310215247 1104 4 0.3162117428607729 1104 5 0.2739898615918861 1104 6 0.3798903832778251 1104 7 0.3854308980857084 1104 8 0.16974652981521388 1104 9 0.17176605601754602 1104 10-11 0.35067350045448364 1104 12-13 0.27714161674862225 1104 14-15 0.43062526488447617 1104 16-17 0.47299220778648987 1104 18-19 0.5931999060131545 1104 20-21 0.6742863332410352 1104 22-23 0.26536253959609724 1104 24-25 0.37355603297918094 1104 26-27 0.28578142488398584 1104 28-29 0.3716912572365203 1104 30-31 0.33327630291038446 1104 32-33 0.5336203046238026 1104 34-35 0.5078944809567147 1104 36-37 0.40836735443441086 1104 38-39 0.7050123960928047 1104 40-41 0.5065007255193592 1104 42-43 0.5407502740912449 1104 44-45 0.6246830112000552 1104 46-47 0.9481741280703417 1104 48-49 0.8453035619672775 1104 50-51 0.6950011943831029 1104 52-53 0.5798706209567897 1104 54-55 0.7377919630964058 1104 56-57 0.6814121033988201 1104 58-59 0.8135910899472591 1104 60-61 0.7803573455729875 1104 62-63 0.7858457735015278 1104 64-65 0.7961630715868786 1104 66-67 0.6369114989095017 1104 68-69 0.8291830272626015 1104 70-71 0.97974421354143 1104 72-73 0.6572902610462101 1104 74-75 0.5300847577151764 1104 76-77 0.41862258909994665 1104 78-79 0.40584076480692843 1104 80-81 0.4649023587845633 1104 82-83 0.390534163438474 1104 84-85 0.40070540688663314 1104 86-87 0.34705535204101423 1104 88-89 0.5493992440627622 1104 90-91 0.7617529263239753 1104 92-93 0.45748490775811135 1104 94-95 0.6403223098183979 1104 96-97 0.6149275953507889 1104 98-99 0.6691877208008279 1104 100-101 0.898937760662271 1105 1 0.017700587793502365 1105 2 0.2056910261312126 1105 3 0.19091061408016685 1105 4 0.13065435521358637 1105 5 0.3421933060855622 1105 6 0.19242988018707763 1105 7 0.230875406290167 1105 8 0.12116623940851667 1105 9 0.37039465069027244 1105 10-11 0.27272930096149395 1105 12-13 0.2007736617260676 1105 14-15 0.0647240816115584 1105 16-17 0.13892825729654845 1105 18-19 0.09058312227826093 1105 20-21 0.3047895692636686 1105 22-23 0.27851546436492214 1105 24-25 0.35684203055307506 1105 26-27 0.34696622320460335 1105 28-29 0.32517206991040126 1105 30-31 0.030148874077461585 1105 32-33 0.027090148960581928 1105 34-35 0.1613004764967414 1105 36-37 0.3234517234644656 1105 38-39 0.3881000919429951 1105 40-41 0.31473460533253217 1105 42-43 0.21063238262694028 1105 44-45 0.09685176378777527 1105 46-47 0.07270107313423324 1105 48-49 0.45817392493933085 1105 50-51 0.496356256161711 1105 52-53 0.46584894577936353 1105 54-55 0.3161629108043158 1105 56-57 0.5693552859598938 1105 58-59 0.5821791526193252 1105 60-61 0.39975968015810537 1105 62-63 0.2577021827149295 1105 64-65 0.5242826918354417 1105 66-67 0.6156085912105986 1105 68-69 0.944090885967185 1105 70-71 0.6708729353306495 1105 72-73 0.8961730160600503 1105 74-75 0.974985207344357 1105 76-77 1.040245830352518 1105 78-79 0.9813316160515768 1105 80-81 0.9217735498297515 1105 82-83 0.8605546473527887 1105 84-85 0.6578784087970106 1105 86-87 0.536816663592635 1105 88-89 0.7230421847393131 1105 90-91 1.0695745855210355 1105 92-93 1.2962648800103977 1105 94-95 0.8167727089641765 1105 96-97 0.7388371174654296 1105 98-99 0.8730970976394801 1105 100-101 1.216859732929084 1106 1 0.31716938233752856 1106 2 0.13512640835622847 1106 3 0.2972617046784265 1106 4 0.18091491630221412 1106 5 0.011558013693395708 1106 6 0.19508819912118014 1106 7 0.1998968240908212 1106 8 0.24177600277229772 1106 9 0.36541259434307705 1106 10-11 0.4762419486013201 1106 12-13 0.23740575496889704 1106 14-15 0.4284439864620708 1106 16-17 0.4301745963788761 1106 18-19 0.21704347895132514 1106 20-21 0.3023649990634212 1106 22-23 0.4374943948957224 1106 24-25 0.43350932360523586 1106 26-27 0.16998864016650828 1106 28-29 0.3946779434202483 1106 30-31 0.6127492599920359 1106 32-33 0.6335191558034623 1106 34-35 0.501017594695675 1106 36-37 0.45335185317210147 1106 38-39 0.7851337746772131 1106 40-41 0.6183421274584546 1106 42-43 0.7496225880966563 1106 44-45 0.5862505712109041 1106 46-47 0.7944690385577573 1106 48-49 0.9231161981686782 1106 50-51 0.7963658811095797 1106 52-53 0.9720134957213915 1106 54-55 0.8479023222158375 1106 56-57 0.8041948472455971 1106 58-59 0.9232162636683015 1106 60-61 0.9933989646119841 1106 62-63 1.1379999144355466 1106 64-65 1.1070641184360461 1106 66-67 1.0162997541334668 1106 68-69 0.8593775948308391 1106 70-71 0.6808576729133229 1106 72-73 0.8237109085631324 1106 74-75 0.6697276941558883 1106 76-77 0.7043947328792939 1106 78-79 0.7569649147045467 1106 80-81 0.9305528772750584 1106 82-83 0.8758285229526308 1106 84-85 0.9284288183156235 1106 86-87 0.9912150356014529 1106 88-89 1.0702025959537522 1106 90-91 1.2774803343648955 1106 92-93 1.3186314255249414 1106 94-95 1.0560007881454148 1106 96-97 1.0553443613845204 1106 98-99 0.9403159002042969 1106 100-101 0.9416916400895872 1107 1 0.03680161932203774 1107 2 -0.09286501600394814 1107 3 0.13621593013941435 1107 4 -0.008542818780604478 1107 5 -0.0432096091433678 1107 6 0.04561232326181397 1107 7 0.143382120785283 1107 8 0.2599992373964426 1107 9 0.20861856430772718 1107 10-11 0.05954521909472277 1107 12-13 0.1870032564892128 1107 14-15 -0.0132710384299628 1107 16-17 0.07121655723190656 1107 18-19 -0.05846956488544919 1107 20-21 0.30597769348410253 1107 22-23 0.13886989866301747 1107 24-25 0.25369145287888273 1107 26-27 0.29510073161251427 1107 28-29 0.0415950421328759 1107 30-31 0.15951329590253494 1107 32-33 0.5071856541182314 1107 34-35 0.45810847380183617 1107 36-37 0.6884243647419979 1107 38-39 0.15551225724248496 1107 40-41 0.4996153832124861 1107 42-43 0.5095252875678966 1107 44-45 0.564164691125562 1107 46-47 0.7723651207684483 1107 48-49 0.776887109021196 1107 50-51 0.7564097900346312 1107 52-53 0.7443184050690448 1107 54-55 0.9041387295174914 1107 56-57 0.8251752737599958 1107 58-59 0.8954433532074617 1107 60-61 1.0201926854295564 1107 62-63 0.572914724936588 1107 64-65 0.6090173440928801 1107 66-67 0.47485684088966806 1107 68-69 0.5263423130819049 1107 70-71 0.6140614783534915 1107 72-73 0.7369985120222502 1107 74-75 0.6094663020574309 1107 76-77 0.7167505600883999 1107 78-79 0.7323717937694667 1107 80-81 0.7546053824544607 1107 82-83 0.7457663701784298 1107 84-85 0.6530781138144448 1107 86-87 0.8533812092375435 1107 88-89 1.020073239848248 1107 90-91 1.128530128735072 1107 92-93 0.9612297825477931 1107 94-95 0.730162550274482 1107 96-97 0.5556793473151131 1107 98-99 0.7597017936890822 1107 100-101 0.5392215053737068 1108 1 0.35379194892788846 1108 2 0.2517880571954052 1108 3 0.06278525343750374 1108 4 0.12034311119084862 1108 5 0.16357546437193804 1108 6 0.1124587817761622 1108 7 0.21919936500505344 1108 8 0.5118695615859679 1108 9 0.11895584328223663 1108 10-11 0.2770218894098946 1108 12-13 0.1942306630858468 1108 14-15 0.35699391005854153 1108 16-17 0.15300022024631232 1108 18-19 0.3641898499259355 1108 20-21 0.40487637438366164 1108 22-23 0.23358503390184637 1108 24-25 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2316 54-55 -0.5086121696587398 2316 56-57 -0.803900024998562 2316 58-59 -0.6538663114868157 2316 60-61 -0.8597817895675419 2316 62-63 -0.9758728374256371 2316 64-65 -0.9710383468225423 2316 66-67 -0.48508630796750296 2316 68-69 -1.03030578994629 2316 70-71 -0.777805327540463 2316 72-73 -1.0160180792296352 2316 74-75 -1.0726894004602592 2316 76-77 -1.1181780859262176 2316 78-79 -1.257940307402361 2316 80-81 -1.1797547521319203 2316 82-83 -1.1781807234519235 2316 84-85 -1.3164369111304453 2316 86-87 -1.7176827192924442 2316 88-89 -1.533471254872719 2316 90-91 -1.0731754055496516 2316 92-93 -1.293179081589578 2316 94-95 -2.210201761130552 2316 96-97 -2.3558533130863246 2316 98-99 -2.3683873117755923 2316 100-101 -2.713563040397265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 691.0 3 243.0 4 229.0 5 232.0 6 298.0 7 331.0 8 430.0 9 574.0 10 814.0 11 1265.0 12 2106.0 13 3088.0 14 3533.0 15 5002.0 16 6951.0 17 10738.0 18 8936.0 19 4020.0 20 3099.0 21 2753.0 22 2730.0 23 2923.0 24 3148.0 25 3553.0 26 3996.0 27 4551.0 28 5129.0 29 6214.0 30 7631.0 31 9472.0 32 12218.0 33 16564.0 34 23171.0 35 33675.0 36 52135.0 37 82182.0 38 86309.0 39 17326.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42967693410914 19.418757022029023 15.680059423008197 20.471506620853646 2 15.530631263999478 20.84873908361965 41.7920731721559 21.82855648022497 3 17.862789747182813 26.84486483330414 33.48841011268757 21.80393530682548 4 11.398519563577246 18.273275763401127 40.29015432697137 30.03805034605026 5 11.38809324667193 38.46658847827001 35.226598384210305 14.91871989084775 6 26.661355438012823 38.82539296861858 19.38138982860774 15.131861764760854 7 24.500714933226174 36.794762763660835 21.730699139276467 16.973823163836528 8 26.003794162036886 32.41018068995808 21.069047207412634 20.516977940592394 9 27.666985958928116 15.353363082026961 22.116206807934024 34.863444151110905 10-11 23.591175388281748 26.911794769951463 28.639217770716634 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19.951453533512588 25.37854685177683 35.24522503820787 19.424774576502717 38-39 19.123021275303977 26.17684352108499 35.38026307493642 19.31987212867461 40-41 19.455066196053338 26.17228137321637 35.126349870573556 19.246302560156735 42-43 19.38769195922461 26.262321992955023 34.57571947128828 19.774266576532085 44-45 19.975995834296537 26.32087124847347 33.832447187332896 19.870685729897094 46-47 20.038318336409773 26.537607894371224 33.08780176273688 20.336272006482126 48-49 20.283169764091355 26.899609815555543 32.613558617565545 20.203661802787565 50-51 20.429546343485292 27.511748600384127 31.47644150218626 20.58226355394432 52-53 20.761310313988258 27.62368111038334 30.95317321601827 20.661835359610134 54-55 21.036230471144403 27.191942920457823 30.8537529378661 20.91807367053167 56-57 21.132355723083606 27.932305277834217 30.260960748458032 20.67437825062414 58-59 21.013247337338903 27.566978098576257 30.368464191557475 21.05131037252736 60-61 20.90724071455511 27.086794929129482 31.155121137851594 20.850843218463815 62-63 20.826546446824086 26.877938794835778 30.961396773353606 21.33411798498653 64-65 20.83440849166864 26.93227845219233 31.29930890184398 20.93400415429505 66-67 20.95975488862448 27.374312112999704 30.52052305683964 21.145409941536172 68-69 20.8900410455822 26.988024965989972 30.74845422220665 21.37347976622118 70-71 20.375910175753244 27.32325426072011 31.258786494603164 21.042049068923482 72-73 20.91788003689735 26.958887474749833 30.98385156988896 21.139380918463857 74-75 20.325590573706844 27.314041239448724 30.692551374161763 21.667816812682666 76-77 20.014619609894627 27.317535124430165 30.995441297361932 21.67240396831328 78-79 20.099057655946908 27.37562611749345 30.621451903549 21.903864323010644 80-81 19.87023557754627 26.967807632885332 30.920881305024633 22.241075484543764 82-83 19.979166593679675 27.695818254221276 30.626275082708858 21.69874006939019 84-85 20.16646193654456 27.030447961962235 30.175663115416413 22.627426986076795 86-87 19.92349199713679 27.20471378977348 30.149314382266773 22.722479830822966 88-89 20.023679942458205 26.713030156897133 30.559619626328637 22.70367027431602 90-91 19.743513097576184 26.694207735007907 31.072560361879365 22.489718805536548 92-93 19.888570474328702 26.667063711717265 31.12693063155854 22.31743518239549 94-95 19.66762496175772 27.221730489431117 30.636094640008782 22.47454990880238 96-97 19.906781303940598 27.20498069525124 30.150911048669453 22.7373269521387 98-99 19.718999152021716 26.84365195981097 30.098557035299724 23.33879185286759 100-101 20.25822745182328 27.155351158262146 29.90747219551741 22.67894919439717 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 305.0 1 212.5 2 126.0 3 145.0 4 137.0 5 139.0 6 224.0 7 423.0 8 772.0 9 972.0 10 873.5 11 815.0 12 849.5 13 915.5 14 977.0 15 1029.0 16 1128.5 17 1282.5 18 1478.0 19 1598.0 20 1743.0 21 1993.0 22 2296.5 23 2584.5 24 2855.5 25 3472.5 26 4912.5 27 6629.0 28 7059.5 29 7516.0 30 8842.0 31 9844.0 32 11018.5 33 12367.0 34 14063.0 35 15237.0 36 15679.5 37 15988.0 38 14101.0 39 12007.0 40 11655.5 41 11755.5 42 12007.0 43 12530.5 44 13020.0 45 12671.0 46 12385.0 47 12586.0 48 13416.0 49 13498.5 50 12508.5 51 11554.5 52 10923.0 53 10657.0 54 9831.0 55 9019.0 56 8390.0 57 7790.5 58 7397.5 59 6552.5 60 5545.5 61 4645.0 62 3684.5 63 2655.0 64 1784.5 65 1418.5 66 1097.0 67 730.5 68 540.0 69 403.5 70 290.0 71 211.5 72 155.0 73 137.0 74 105.0 75 83.0 76 70.0 77 36.5 78 16.5 79 12.5 80 11.5 81 7.0 82 4.0 83 1.5 84 0.5 85 1.5 86 2.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03409120096762971 2 0.028487167931854955 3 0.020548121131174067 4 0.03409120096762971 5 0.055339826228275615 6 0.05580682898125685 7 0.05860884549914422 8 0.055339826228275615 9 0.05440582072231316 10-11 0.05965960169335198 12-13 0.05697433586370991 14-15 0.05312156315161478 16-17 0.04343125602725428 18-19 0.03420795165587501 20-21 0.04097949157410283 22-23 0.021482126637136525 24-25 0.011091315383304184 26-27 0.008989802994888656 28-29 0.01167506882453072 30-31 0.00793904680068089 32-33 0.005720783724020053 34-35 0.006187786477001282 36-37 0.0031522685826232944 38-39 0.004670027529812288 40-41 0.006421287853491896 42-43 0.0033857699591139092 44-45 9.340055059624576E-4 46-47 0.0019847617001702224 48-49 9.340055059624576E-4 50-51 0.004786778218057596 52-53 0.0033857699591139092 54-55 0.004319775465076367 56-57 0.017746104613286698 58-59 0.006071035788755975 60-61 0.012258822265757256 62-63 0.011091315383304184 64-65 0.007355293359454354 66-67 0.011558318136285413 68-69 0.01879686080749446 70-71 0.011441567448040106 72-73 0.011208066071549491 74-75 0.013192827771719715 76-77 0.016578597730833623 78-79 0.030004926879043953 80-81 0.023933891090287977 82-83 0.024400893843269204 84-85 0.013309578459965022 86-87 0.016928849795569544 88-89 0.012258822265757256 90-91 0.012609074330493178 92-93 0.02335013764906144 94-95 0.016812099107324237 96-97 0.03035517894377987 98-99 0.04343125602725428 100-101 0.053588565904596014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 428263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.58882233104404 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49611075734704 47.508538654069895 2 10.756201818073265 12.388739909157485 3 2.9605462938323273 5.114831235550224 4 1.1876372759834224 2.735785283213377 5 0.6025678965013581 1.7350587767003822 6 0.3625638058228585 1.252777355831985 7 0.2525671474603515 1.0181531207226953 8 0.1722015660069925 0.7933508311923394 9 0.13293997136727756 0.6890270752587807 >10 0.8861285028578041 10.17047112366037 >50 0.11148322405576294 4.45024057116189 >100 0.07499780527142706 8.04881407656847 >500 0.002837754794053997 1.013455792620482 >1k 8.107870840154277E-4 1.6890929877008032 >5k 4.0539354200771387E-4 1.391663206590807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5961 1.3919017052605525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4408 1.0292740675706282 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2827 0.6601083913389669 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.22976535446676458 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 611 0.1426693410357654 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 584 0.13636480387051883 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 576 0.1344967928585939 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 556 0.1298267653287816 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 524 0.12235472128108195 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 506 0.11815169650425089 No Hit GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC 500 0.1167506882453072 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 484 0.11301466622145738 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 433 0.10110609602043605 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 429 0.10017209051447358 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.167506882453072E-4 0.0 16-17 0.0 0.0 0.0 2.335013764906144E-4 0.0 18-19 0.0 0.0 0.0 2.335013764906144E-4 0.0 20-21 0.0 0.0 0.0 4.670027529812288E-4 0.0 22-23 0.0 0.0 0.0 0.0018680110119249152 0.0 24-25 0.0 0.0 0.0 0.0032690192708686016 0.0 26-27 0.0 0.0 0.0 0.004670027529812288 0.0 28-29 0.0 0.0 0.0 0.008172548177171505 0.0 30-31 0.0 0.0 0.0 0.015994844289607086 0.0 32-33 0.0 0.0 0.0 0.023350137649061443 0.0 34-35 0.0 0.0 0.0 0.030121677567289257 0.0 36-37 0.0 0.0 0.0 0.03607596266779993 0.0 38-39 0.0 0.0 0.0 0.04576626979216042 0.0 40-41 0.0 0.0 0.0 0.056740834487219304 0.0 42-43 0.0 0.0 0.0 0.07974072007154481 0.0 44-45 0.0 0.0 0.0 0.11756794306302436 0.0 46-47 0.0 0.0 0.0 0.15014138508346508 0.0 48-49 0.0 0.0 0.0 0.22322731592502737 0.0 50-51 0.0 0.0 0.0 0.2953792412606272 0.0 52-53 0.0 0.0 0.0 0.3794397367972484 0.0 54-55 0.0 0.0 0.0 0.5521140047120577 0.0 56-57 0.0 0.0 0.0 0.7007376308483338 0.0 58-59 0.0 0.0 0.0 0.884736715522938 0.0 60-61 0.0 0.0 0.0 1.0947712036762458 0.0 62-63 0.0 0.0 0.0 1.377307869229889 0.0 64-65 0.0 0.0 0.0 1.5782358037000628 0.0 66-67 0.0 0.0 0.0 1.8585542061770455 0.0 68-69 0.0 0.0 0.0 2.219897586296271 0.0 70-71 0.0 0.0 0.0 2.440322885703411 0.0 72-73 0.0 0.0 0.0 2.8006155096284293 0.0 74-75 0.0 0.0 0.0 3.2476538949197105 0.0 76-77 0.0 0.0 0.0 3.5764238330184956 0.0 78-79 0.0 0.0 0.0 3.859310750636875 0.0 80-81 0.0 0.0 0.0 4.293973562974154 0.0 82-83 0.0 0.0 0.0 4.828925216514151 0.0 84-85 0.0 0.0 0.0 5.426688740330125 0.0 86-87 0.0 0.0 0.0 6.2999838884050225 0.0 88-89 0.0 0.0 0.0 7.222314325542949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2285 0.0 49.06475 1 GTATCAA 4120 0.0 43.23922 1 TATCAAC 5245 0.0 33.968807 2 TATAGAG 85 1.6272295E-5 33.537205 5 ATCAACG 5355 0.0 33.182312 3 TCAACGC 5370 0.0 33.089626 4 CAACGCA 5425 0.0 32.754154 5 AACGCAG 5530 0.0 32.042583 6 TACTAGG 60 0.0039437166 31.674028 2 CGTCACT 90 2.4075493E-5 31.670328 1 ACGCAGA 6030 0.0 29.228088 7 CGCAGAG 6060 0.0 28.929989 8 GCAGAGT 6280 0.0 27.386938 9 TAGATTG 70 0.008376289 27.149166 5 TGACGAG 35 0.008392836 27.14124 60-61 GAGTACT 4090 0.0 25.323734 12-13 GTACATG 2670 0.0 25.26509 1 TACATGG 2625 0.0 25.158228 2 CGGGACT 95 0.001223638 25.00581 4 TCACTAC 135 1.3377576E-5 24.635353 3 >>END_MODULE