FastQCFastQC Report
Thu 26 May 2016
SRR937038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428263
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43931.025771546923269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.8427064677546274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.5368196645519225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.19730866313456916No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8190.1912376273458132No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6540.15270990022486183No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5630.13146127496421592No Hit
ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT5270.1230552254105538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.12282172403406318No Hit
ATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCAAAA5190.12118721439862887No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG5130.11978620613968519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.11745119237477905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.10974564695058876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4580.10694363043270141No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4440.1036746111618328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA250.00502142656.9934167
GGTATCA25800.045.127721
GTATCAA42050.036.842461
GTGTAGG902.4017496E-531.6815031
ATGGTCG650.00584333129.227396
TATCAAC54400.028.2970832
TCAACGC55950.027.5067374
ATCAACG56000.027.4853843
CAACGCA56550.027.2988835
GCCGTTC1056.8739275E-527.1555751
AACGCAG58050.026.5085666
GTACATG35850.025.5838071
TACATGG35800.025.2154162
ACATGGG36050.025.037633
ATGGGAT4500.024.2778075
AGTACTT37250.023.97038512-13
ATAGCCC1000.001650822623.7528043
ACGCAGA64950.023.5461647
GAGTACT36850.023.45726212-13
CGCAGAG65200.023.3830348
Produced by FastQC (version 0.11.5)