FastQCFastQC Report
Thu 26 May 2016
SRR937037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453640
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61441.35437792081827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44200.9743408870469976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.6154660082885107No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.21999823648708228No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA6320.13931752050083768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.12719336919142932No Hit
GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC5680.12520941715898068No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5620.12388678247068159No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5500.12124151309408343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5350.11793492637333569No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5320.11727360902918614No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5210.11484877876730447No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT4610.10162243188431355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.046.894291
GTATCAA42500.043.1465841
TATCAAC54250.033.801472
ATCAACG55800.032.7774053
TCAACGC56450.032.315834
CAACGCA57450.031.8360165
AACGCAG58350.031.4298556
GTGTAGG1153.854024E-628.9166681
ACGCAGA65600.027.8838567
CGCAGAG66100.027.45738
ACTATAC1754.9367372E-927.146263
TCGGTAC350.00838552627.1462690-91
ATATAGA1257.370818E-626.6033341
GTCGGTA458.9395436E-426.39219790-91
GCAGAGT67300.026.1176879
TACATGG32450.026.0587372
GTACATG32850.026.030661
ACATGGG31600.025.5570013
CATGGGT4650.024.5192014
CTATACC1751.4802208E-724.4316334
Produced by FastQC (version 0.11.5)