FastQCFastQC Report
Thu 26 May 2016
SRR937037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453640
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44520.9813949387179262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.8434000529053876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.5140640155189137No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.20941715898068952No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8380.18472797813243982No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6750.1487964024336478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.13005907768274402No Hit
ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT5510.12146195220879992No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG5450.12013931752050083No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5340.11771448725861916No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.11088087470240719No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT4980.10977867912882462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.10889692266995855No Hit
ATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCAAAA4560.10052023631073098No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCA200.002072195871.259267
GGTATCA29250.045.481011
TGAACCG459.6127496E-442.227715
GTATCAA48350.036.8495981
ACCGAAC702.049814E-433.9329838
GTATAGG851.6273549E-533.5374681
TATCAAC61200.028.9475422
TCAACGC61650.028.8132884
ATCAACG61550.028.7829363
CAACGCA63200.028.1885
AACGCAG64800.027.6386226
CGCAGAA700.00838099427.1463858
ATATCGT350.008394361527.140494-95
TTTGCGT700.00839001427.14043
ATATACT1109.47006E-525.9067464
ATAGATT6800.025.1447854
TACATGG38550.025.1339132
GTACATG38700.025.0447751
ACGGGCG1351.3390487E-524.632836
ACGCAGA73150.024.4837027
Produced by FastQC (version 0.11.5)