FastQCFastQC Report
Thu 26 May 2016
SRR937036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480698
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63531.321619811191226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.976080616104082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28900.6012090751365723No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.21780827047335333No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA6760.1406288355682778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.13105941776333582No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6230.12960320201040987No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5690.11836953763069537No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5590.1162892294122297No Hit
GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC5400.11233664379714499No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT5190.10796799653836713No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5170.107551934894674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.10671981160728773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4990.10380738010143584No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT4970.10339131845774269No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC4810.10006282530819766No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG250.005015105557.012627
GGTATCA23500.053.805871
TGCACCG502.808694E-547.5451665
GTATCAA44900.046.158961
TATCAAC57100.036.3836672
ATCAACG58250.035.5837443
TCAACGC58600.035.37494
CAACGCA59900.034.527795
TATAGGA1255.5915734E-934.228952
AACGCAG61050.033.8668066
GTATAGG753.0624392E-431.6901761
GTCGGTA300.003947359531.6720390-91
GTGTAGG1252.1355663E-730.4225651
ACGCAGA68600.029.9191597
CGCAGAG69100.029.7026678
GCAGAGT70950.028.2526639
TCGGTAC350.0083839727.14745390-91
TGGTATC9400.026.2988412
GTGGTAT9400.026.29611
AGGACCG1109.404395E-525.9337275
Produced by FastQC (version 0.11.5)