##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480698 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.953245072789983 33.0 31.0 34.0 30.0 34.0 2 32.02347627824538 33.0 31.0 34.0 30.0 34.0 3 32.00393594314934 33.0 31.0 34.0 30.0 34.0 4 35.64121132186945 37.0 35.0 37.0 33.0 37.0 5 35.6678496686069 37.0 35.0 37.0 33.0 37.0 6 35.762301902649895 37.0 35.0 37.0 35.0 37.0 7 35.727639807113825 37.0 35.0 37.0 35.0 37.0 8 35.69236194034508 37.0 35.0 37.0 33.0 37.0 9 37.32446983345052 39.0 37.0 39.0 34.0 39.0 10-11 37.22203545677328 39.0 37.0 39.0 34.0 39.0 12-13 37.14444516099505 39.0 37.0 39.0 33.5 39.0 14-15 38.2390388559969 40.0 38.0 41.0 33.5 41.0 16-17 38.207571073730286 40.0 38.0 41.0 33.0 41.0 18-19 38.287799200329516 40.0 38.0 41.0 33.5 41.0 20-21 38.27392354451236 40.0 38.0 41.0 34.0 41.0 22-23 38.170360600626594 40.0 38.0 41.0 34.0 41.0 24-25 38.09634843498412 40.0 38.0 41.0 33.5 41.0 26-27 38.05008134005134 40.0 38.0 41.0 33.0 41.0 28-29 37.92476461312508 40.0 38.0 41.0 33.0 41.0 30-31 37.63279647512576 40.0 37.0 41.0 33.0 41.0 32-33 37.26586651078224 39.5 35.5 41.0 33.0 41.0 34-35 37.02039014100329 39.0 35.0 41.0 32.0 41.0 36-37 36.77021227465062 39.0 35.0 41.0 31.0 41.0 38-39 36.50112544674619 39.0 35.0 41.0 31.0 41.0 40-41 36.217232025096834 39.0 35.0 41.0 30.0 41.0 42-43 35.821569883793984 39.0 35.0 41.0 29.5 41.0 44-45 35.243888054454146 39.0 35.0 40.5 26.5 41.0 46-47 34.49522257217629 38.0 34.5 40.0 21.5 41.0 48-49 33.895146224864675 38.5 34.0 40.0 11.5 41.0 50-51 33.057893937565794 38.0 33.5 39.5 6.0 40.5 52-53 33.18331884051941 38.0 33.5 40.0 2.0 40.5 54-55 33.49633761738139 39.0 34.0 40.5 2.0 41.0 56-57 33.3486554967984 38.0 34.0 41.0 2.0 41.0 58-59 33.130661038739504 38.0 34.0 40.0 2.0 41.0 60-61 32.86647853746011 38.0 33.5 40.0 2.0 41.0 62-63 32.566665557168946 37.5 33.0 40.0 2.0 41.0 64-65 32.25431976001565 37.0 33.0 40.0 2.0 41.0 66-67 31.892923415533247 36.5 32.0 39.5 2.0 41.0 68-69 31.4873933321961 36.0 32.0 39.0 2.0 41.0 70-71 31.098400659041644 35.5 31.0 39.0 2.0 40.5 72-73 30.737407894353627 35.0 31.0 38.5 2.0 40.0 74-75 30.349402327448836 35.0 31.0 37.0 2.0 39.5 76-77 29.944043869539712 35.0 30.5 37.0 2.0 39.0 78-79 29.518787263520963 35.0 30.0 36.0 2.0 39.0 80-81 29.106073875905455 35.0 29.5 36.0 2.0 38.5 82-83 28.73056888108542 34.5 29.0 35.5 2.0 37.0 84-85 28.40706743111059 34.0 29.0 35.0 2.0 37.0 86-87 28.09030826007181 34.0 29.0 35.0 2.0 36.0 88-89 27.74370810779325 34.0 27.5 35.0 2.0 36.0 90-91 27.416300879138255 34.0 27.0 35.0 2.0 36.0 92-93 27.086629234987456 34.0 26.0 35.0 2.0 35.0 94-95 26.75815272790817 34.0 25.5 35.0 2.0 35.0 96-97 26.350594344058017 34.0 25.0 35.0 2.0 35.0 98-99 25.910685087102507 34.0 23.5 35.0 2.0 35.0 100-101 24.057550686709742 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28453071810224984 1101 2 0.37312791404362855 1101 3 0.33204650497764376 1101 4 0.04058052490334063 1101 5 0.2366782125168001 1101 6 0.06654797570545412 1101 7 0.10944429387901522 1101 8 0.11612101749529558 1101 9 0.10285911681771864 1101 10-11 0.2107511045958077 1101 12-13 0.22871982297987614 1101 14-15 0.3767161944417339 1101 16-17 0.23975879995590788 1101 18-19 0.3414190640845618 1101 20-21 0.3225486024836357 1101 22-23 0.4510134669206849 1101 24-25 0.5950398382493773 1101 26-27 0.3493277881036718 1101 28-29 0.3502221491357318 1101 30-31 0.5482315409418277 1101 32-33 0.6731656773663062 1101 34-35 0.7467466266867078 1101 36-37 0.791315330766281 1101 38-39 0.537029208299991 1101 40-41 0.7856724720648103 1101 42-43 0.6502036791709287 1101 44-45 1.0801530490430906 1101 46-47 0.8457701989678981 1101 48-49 1.1064784566975234 1101 50-51 0.96396858664761 1101 52-53 1.0355051203786942 1101 54-55 1.294639460466641 1101 56-57 1.2891149395905899 1101 58-59 1.1807640241538522 1101 60-61 1.1911697991838892 1101 62-63 1.3349421834766702 1101 64-65 1.1917523066068938 1101 66-67 1.1073155357165803 1101 68-69 1.0979938712452473 1101 70-71 0.9580262203197378 1101 72-73 0.8949300746672115 1101 74-75 0.8718022201023139 1101 76-77 0.8991115453657521 1101 78-79 0.9184892263199096 1101 80-81 1.1571863221300696 1101 82-83 0.9506879345471795 1101 84-85 0.9993858124352286 1101 86-87 1.0325854873016347 1101 88-89 0.9707202942509063 1101 90-91 1.026773932478605 1101 92-93 1.2692400182648065 1101 94-95 1.2398860407628476 1101 96-97 1.112287851861307 1101 98-99 1.0673421459105015 1101 100-101 1.3414904827585197 1102 1 0.42581045208699564 1102 2 0.22666905631162848 1102 3 0.3044845756240129 1102 4 0.25030717194085383 1102 5 0.2707177618513015 1102 6 0.3538147247278687 1102 7 0.2721984434011375 1102 8 0.12716659489886695 1102 9 0.3446692094214896 1102 10-11 0.19835315915494078 1102 12-13 0.31324654060802715 1102 14-15 0.24129641234389254 1102 16-17 0.47763067794555525 1102 18-19 0.33059268919253526 1102 20-21 0.28592599304492694 1102 22-23 0.10829498424789819 1102 24-25 0.0036890492132073405 1102 26-27 0.07176725198633704 1102 28-29 -0.2553814472715814 1102 30-31 -0.016200993912299566 1102 32-33 -0.12001210887408575 1102 34-35 -0.07901431254032332 1102 36-37 0.11498548671586661 1102 38-39 0.0831518316710671 1102 40-41 0.04879443597069866 1102 42-43 0.24513601432785492 1102 44-45 0.16431938288108938 1102 46-47 0.23346606477137755 1102 48-49 0.6078642624060748 1102 50-51 0.5252756280638806 1102 52-53 0.7832847661251421 1102 54-55 0.38857609607961763 1102 56-57 0.5490454635403168 1102 58-59 0.4548489001093756 1102 60-61 0.4117051734535906 1102 62-63 0.5791114895521048 1102 64-65 0.6436921559516051 1102 66-67 0.3009642358271165 1102 68-69 0.5409092646487359 1102 70-71 0.28718015435464395 1102 72-73 0.6115746043570773 1102 74-75 0.7848484848357877 1102 76-77 0.5997635799551695 1102 78-79 0.5956559409758242 1102 80-81 0.4500845322858318 1102 82-83 0.7493857230167578 1102 84-85 0.4405373686801788 1102 86-87 0.5693594950851839 1102 88-89 0.7571891832842788 1102 90-91 0.7043307532991285 1102 92-93 1.0644073459446979 1102 94-95 0.9522728403132952 1102 96-97 0.8405306093545946 1102 98-99 1.0160143917145348 1102 100-101 0.7799865306115414 1103 1 0.10042475783734872 1103 2 0.1796231110563724 1103 3 0.04319646649673814 1103 4 0.12184839952209359 1103 5 -0.03292377138772906 1103 6 0.105750993368666 1103 7 0.31695044607337763 1103 8 0.18357947236812322 1103 9 0.06076765577844867 1103 10-11 -0.006154840466756184 1103 12-13 0.11368661538251246 1103 14-15 0.27959418621346543 1103 16-17 0.3734253177663902 1103 18-19 0.42341285013072394 1103 20-21 0.1893419552632878 1103 22-23 0.2357446231531597 1103 24-25 0.3576373469669605 1103 26-27 0.13441904961231188 1103 28-29 0.3152719842854239 1103 30-31 0.38571384447541845 1103 32-33 0.5571899250883376 1103 34-35 0.6286377969813444 1103 36-37 0.818576915419527 1103 38-39 0.7370140059664365 1103 40-41 0.5527062117438462 1103 42-43 0.6838796984398812 1103 44-45 0.840644084417022 1103 46-47 0.6319208730963766 1103 48-49 0.9695415368803282 1103 50-51 0.9858409440544733 1103 52-53 1.0487482531995838 1103 54-55 0.8833070709448521 1103 56-57 1.2267103104800228 1103 58-59 1.3652794972789764 1103 60-61 1.2634188894192349 1103 62-63 1.1969144962266753 1103 64-65 1.1635880833986292 1103 66-67 1.0135855481634977 1103 68-69 0.9865408132008362 1103 70-71 1.1617165867909875 1103 72-73 0.9689929933252266 1103 74-75 0.9909904154903053 1103 76-77 1.0874276018804316 1103 78-79 1.096310921950188 1103 80-81 1.087927340876444 1103 82-83 1.0371588078262306 1103 84-85 1.0917333377803686 1103 86-87 1.1065334383121161 1103 88-89 1.1980084829878805 1103 90-91 1.2940993669327945 1103 92-93 1.374396459778577 1103 94-95 1.2905126619491973 1103 96-97 1.0785975804046366 1103 98-99 0.9612545614662871 1103 100-101 1.0210889511234136 1104 1 0.17522150580483142 1104 2 -0.051940733993845356 1104 3 0.05859579696063122 1104 4 -0.03836202611157091 1104 5 0.15521587471461373 1104 6 -0.10677889663611495 1104 7 -0.04316433154498611 1104 8 -0.16964864814837455 1104 9 -0.017950660816481445 1104 10-11 0.03817801462649584 1104 12-13 -0.12375958260888353 1104 14-15 -0.3685645894919318 1104 16-17 -0.10873632737947503 1104 18-19 -0.05190996192093422 1104 20-21 0.24362220394812795 1104 22-23 0.14067049594130765 1104 24-25 0.15610697872210721 1104 26-27 0.19702067562857195 1104 28-29 0.2667784405264513 1104 30-31 0.04855459052994604 1104 32-33 -0.40263790848411674 1104 34-35 -0.1113430734155898 1104 36-37 -0.2296775083011866 1104 38-39 -0.09584108729038121 1104 40-41 0.16375355751141996 1104 42-43 -0.01463775732290884 1104 44-45 -0.2065103871276932 1104 46-47 -0.41695526272381045 1104 48-49 -0.2187894052526289 1104 50-51 -0.28393428310956637 1104 52-53 -0.3131838367095483 1104 54-55 -0.185702972096756 1104 56-57 -0.0754895938719713 1104 58-59 -0.19445268827817586 1104 60-61 -0.10453902545403082 1104 62-63 -0.0843384927738029 1104 64-65 -0.1660628013455181 1104 66-67 -0.1792886746963802 1104 68-69 -0.3001162886643023 1104 70-71 -0.3775469234911739 1104 72-73 -0.3778745341735821 1104 74-75 -0.315991889626865 1104 76-77 -0.1512141963022593 1104 78-79 0.08282455179236692 1104 80-81 -0.3415322478370868 1104 82-83 -0.1928268449714743 1104 84-85 -0.3657032954094923 1104 86-87 -0.20226617909582245 1104 88-89 -0.2628714787529134 1104 90-91 -0.10250512852392646 1104 92-93 -0.24808082381778718 1104 94-95 -0.3965270319771541 1104 96-97 -0.42843254872018477 1104 98-99 -0.18087838927498368 1104 100-101 0.010041605355841199 1105 1 -0.05971152836367466 1105 2 0.08573325944114885 1105 3 0.18109825537533553 1105 4 0.13451646587607513 1105 5 0.07156883381490076 1105 6 -0.07095667810728656 1105 7 -0.0844656985620631 1105 8 -0.1249318159715358 1105 9 -0.015296957959186841 1105 10-11 -0.07180245655811035 1105 12-13 -0.09484031556271333 1105 14-15 0.046703897094126035 1105 16-17 -0.010819401021116448 1105 18-19 -0.05530465174516763 1105 20-21 0.14887282374564137 1105 22-23 0.0780439395224235 1105 24-25 -0.3487973854833797 1105 26-27 0.01865888008557448 1105 28-29 -0.019763297122608492 1105 30-31 0.13466835626725526 1105 32-33 0.2802473701131305 1105 34-35 0.049012046319596436 1105 36-37 -0.09010326417714509 1105 38-39 0.3377817157178882 1105 40-41 0.5010452847728217 1105 42-43 0.4001986022843056 1105 44-45 0.4771588141299077 1105 46-47 0.647658800793316 1105 48-49 0.7895318091349921 1105 50-51 0.5139646379580185 1105 52-53 0.5945639784176606 1105 54-55 0.5372413084533747 1105 56-57 0.5835303643003584 1105 58-59 0.6436091114198916 1105 60-61 0.6672633041535931 1105 62-63 0.6822519976626111 1105 64-65 0.698451540561102 1105 66-67 0.684463904907787 1105 68-69 0.6988366344254082 1105 70-71 0.4380041076579815 1105 72-73 0.6153222359542561 1105 74-75 0.5907254688584764 1105 76-77 0.693420919554864 1105 78-79 0.4521850869650095 1105 80-81 0.5555605902122451 1105 82-83 0.6191480584232174 1105 84-85 0.5337371555537906 1105 86-87 0.5230104791222097 1105 88-89 0.752678167373869 1105 90-91 0.7446479579984242 1105 92-93 0.7459303394625003 1105 94-95 0.8297108317643485 1105 96-97 0.578128302237559 1105 98-99 0.5206866860009818 1105 100-101 0.32546105938002157 1106 1 0.1985458434323384 1106 2 0.1680704682058618 1106 3 0.3812632783327281 1106 4 0.12486760200934555 1106 5 0.09752794084153749 1106 6 0.1702684750902108 1106 7 0.22874360674107663 1106 8 0.00436224853678624 1106 9 0.18394692392136847 1106 10-11 0.16627516824939192 1106 12-13 0.3668967644556318 1106 14-15 0.22476331247184334 1106 16-17 0.29395559515561587 1106 18-19 0.38001630723737634 1106 20-21 0.32250436020184026 1106 22-23 -0.031086382379037047 1106 24-25 0.22697447027802298 1106 26-27 0.23348213836428044 1106 28-29 0.1730414885431628 1106 30-31 0.26661559159924764 1106 32-33 0.5548701112724856 1106 34-35 0.5918682238330817 1106 36-37 0.3565505752740137 1106 38-39 0.2944791937829976 1106 40-41 0.6957154700324324 1106 42-43 0.5975913106880171 1106 44-45 0.5063348018669984 1106 46-47 0.6820806691804009 1106 48-49 1.118542773914676 1106 50-51 1.171319718282227 1106 52-53 0.9887341156641298 1106 54-55 0.9321085254336694 1106 56-57 1.0203112785417687 1106 58-59 1.1464472217647668 1106 60-61 1.345395628381027 1106 62-63 1.0063602868653518 1106 64-65 0.7921767559978647 1106 66-67 0.8960987347100726 1106 68-69 1.0683902544387074 1106 70-71 1.0734723069516576 1106 72-73 1.148138331383052 1106 74-75 1.1126585847693207 1106 76-77 0.9542816707649742 1106 78-79 0.906176046267646 1106 80-81 1.1902836966271586 1106 82-83 1.3098260395949488 1106 84-85 1.4221083718512268 1106 86-87 1.5300844158088758 1106 88-89 1.420647709257338 1106 90-91 1.4702599477698435 1106 92-93 1.3255770338463826 1106 94-95 1.3246943472512562 1106 96-97 1.5796024836134386 1106 98-99 1.6751716472170273 1106 100-101 2.097617347501572 1107 1 0.1391491314136566 1107 2 0.15822649751901707 1107 3 0.3807544354035066 1107 4 0.028382422654097184 1107 5 0.017748978405563776 1107 6 0.09602496283756068 1107 7 0.23157315387086896 1107 8 0.21822845613519348 1107 9 0.172267314256203 1107 10-11 0.2633405236547759 1107 12-13 0.04722213859785285 1107 14-15 0.12299234885649213 1107 16-17 0.3670197331424063 1107 18-19 0.40898230987195205 1107 20-21 0.32914547375941794 1107 22-23 0.2861248730363286 1107 24-25 0.21543990462539142 1107 26-27 0.38698863681160134 1107 28-29 0.2203091922348861 1107 30-31 0.10180754902763312 1107 32-33 0.05491528891573694 1107 34-35 -0.13895386660779963 1107 36-37 0.17869332711559593 1107 38-39 0.05713963386079257 1107 40-41 -0.17855990434757274 1107 42-43 -0.3195861803834461 1107 44-45 -0.5399603936095332 1107 46-47 -0.5582334818841304 1107 48-49 -0.6265602073342151 1107 50-51 -0.8790747538764592 1107 52-53 -0.7156210091450603 1107 54-55 -0.7983925659630842 1107 56-57 -0.3881588162361709 1107 58-59 -0.7047162214374225 1107 60-61 -0.6586846261355532 1107 62-63 -0.6027246994149138 1107 64-65 -0.6363926344843946 1107 66-67 -0.6447941657421765 1107 68-69 -0.8920602855530184 1107 70-71 -0.9605107918542295 1107 72-73 -0.9412373135872514 1107 74-75 -0.7072395515340055 1107 76-77 -0.7508771405399024 1107 78-79 -0.8630446631640183 1107 80-81 -0.7162104707013235 1107 82-83 -0.5674809924241124 1107 84-85 -0.8011904521033841 1107 86-87 -0.7701329124328602 1107 88-89 -0.8181541796437024 1107 90-91 -0.7716330430376921 1107 92-93 -0.5301113069927794 1107 94-95 -0.6985864056460578 1107 96-97 -0.5299770789718643 1107 98-99 -0.40182770127379897 1107 100-101 -0.24617262876827795 1108 1 -0.03750627664540929 1108 2 0.0017672757028854846 1108 3 0.023308275728421535 1108 4 0.17349832778608487 1108 5 0.20271665803888084 1108 6 0.2282958902172325 1108 7 0.1807014973769938 1108 8 0.06103457551955671 1108 9 -0.09111479610895401 1108 10-11 -0.03101507892716171 1108 12-13 -0.012123506816074325 1108 14-15 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scores pass #Quality Count 2 861.0 3 294.0 4 236.0 5 267.0 6 316.0 7 374.0 8 514.0 9 628.0 10 965.0 11 1496.0 12 2414.0 13 3399.0 14 4071.0 15 5637.0 16 7958.0 17 11576.0 18 9802.0 19 4128.0 20 3253.0 21 3027.0 22 3143.0 23 3356.0 24 3570.0 25 3974.0 26 4649.0 27 5308.0 28 6151.0 29 7401.0 30 9126.0 31 11381.0 32 14674.0 33 19427.0 34 27245.0 35 39089.0 36 59424.0 37 91193.0 38 92428.0 39 17939.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48664137625351 19.48013842443362 15.49367288799732 20.539547311315552 2 15.439022157114083 20.834720580646234 41.783680977509945 21.942576284729736 3 17.814783679856173 26.855658948279594 33.47344064721256 21.856116724651674 4 11.540486820059733 18.1898785551497 40.17358184258999 30.096052782200577 5 11.553356819828599 38.344826724633336 34.99591901323406 15.105897442304009 6 26.83645526389771 38.80051228121324 19.40296331774971 14.960069137139348 7 24.76870786528547 36.82462176321097 21.512822273978934 16.89384809752463 8 25.977162247967605 32.461251808595904 21.039044853178446 20.52254109025804 9 27.72471991973322 15.45718341874862 22.045470630602125 34.77262603091603 10-11 23.666825913771337 26.754138082852208 28.64782554843974 20.931210454936714 12-13 25.60425892871082 26.05190439267073 28.991626812932903 19.352209865685545 14-15 22.67018300501224 25.60821274540822 30.192912926914563 21.52869132266498 16-17 19.267414514349934 29.01548419321942 30.117692355720205 21.59940893671044 18-19 19.16998309868536 26.279150604867247 35.38504608314115 19.165820213306247 20-21 20.73046985984445 25.016937933790633 35.582461979412216 18.670130226952704 22-23 20.19297101064743 25.095908028180887 35.318398005570835 19.392722955600853 24-25 19.634016816793075 25.586054732076636 35.7185730588026 19.061355392327687 26-27 19.518936017283686 25.63230143680522 35.813823828311506 19.03493871759959 28-29 19.09444395900688 26.144037647763728 35.62910628170081 19.132412111528588 30-31 19.773732733754205 25.458708200316018 35.819027795548095 18.948531270381686 32-33 19.213249268454554 25.58011319840095 35.71887017967983 19.487767353464662 34-35 19.50613893561269 25.885576439968666 35.23800013106937 19.370284493349267 36-37 20.036595286094418 25.487919810761383 34.906680980229396 19.568803922914807 38-39 19.30273488697215 26.23617057572845 35.02417418729378 19.436920350005618 40-41 19.652044563261313 26.12370361894459 34.895821414082576 19.328430403711526 42-43 19.512530009195192 26.1618811448638 34.36411711595511 19.961471729985895 44-45 20.159397029040758 26.237162890990284 33.550661397813336 20.052778682155626 46-47 20.184005367256614 26.539419691382744 32.80818818683462 20.468386754526023 48-49 20.383547623084418 27.07956059592695 32.19099785415538 20.34589392683325 50-51 20.53194366431588 27.570263579437892 31.263288189893697 20.634504566352536 52-53 20.843614121954772 27.731603803024946 30.693199076289346 20.73158299873094 54-55 21.036255200157292 27.214962721152975 30.63783394658519 21.11094813210454 56-57 21.226940129116553 27.907254952137826 30.054134912857773 20.811670005887848 58-59 21.09855451163797 27.598467133239573 30.23146953822597 21.071508816896486 60-61 20.99294129395048 27.315403290734224 30.817711464728315 20.873943950586977 62-63 20.935953929437808 26.966071227951915 30.6803854747906 21.41758936781968 64-65 20.8804536868296 26.91560359820589 31.232992984996383 20.970949729968126 66-67 21.087971102848307 27.42971820699499 30.253994477747653 21.228316212409045 68-69 20.998262172596437 27.13404164542077 30.4364340197925 21.431262162190286 70-71 20.340704616860812 27.444632117482627 31.061587630646976 21.153075635009593 72-73 21.04046675688636 27.10782778926039 30.75704446943227 21.09466098442098 74-75 20.52214312538946 27.429262776600517 30.392949320117467 21.655644777892558 76-77 20.202956540375432 27.285419859320363 30.677773720311574 21.833849879992634 78-79 20.133591360259693 27.460931810142124 30.499823126703706 21.90565370289448 80-81 20.035725104068103 27.10860868832033 30.58504021676561 22.270625990845964 82-83 20.108996474989908 27.78432098816812 30.36003379348518 21.746648743356793 84-85 20.28805342876165 27.174704560585884 29.948402130492674 22.588839880159785 86-87 20.053415846631808 27.465566859285556 29.86688696108155 22.61413033300109 88-89 20.248016874387996 26.99412996900049 30.170356811722996 22.587496344888518 90-91 19.798795645844542 26.6997428754866 30.864618896934797 22.636842581734065 92-93 19.996212326172827 26.775107907081285 30.925212172168504 22.303467594577384 94-95 19.731774858990388 27.28148451755052 30.483042437078815 22.503698186380273 96-97 19.91800183304707 27.284416707152527 29.99965669379479 22.79792476600561 98-99 19.732896311326154 26.9649862129962 29.998855418552623 23.303262057125018 100-101 20.159345297762734 27.336753823589728 29.93709670535797 22.566804173289572 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 368.0 1 255.5 2 151.5 3 155.5 4 144.5 5 142.5 6 234.0 7 482.5 8 831.5 9 1033.5 10 934.0 11 830.0 12 898.0 13 953.0 14 996.5 15 1127.0 16 1293.0 17 1421.5 18 1538.0 19 1687.0 20 1883.0 21 2152.5 22 2471.0 23 2788.5 24 3178.0 25 3914.0 26 5502.0 27 7328.0 28 7875.5 29 8488.5 30 9878.0 31 10825.0 32 12041.0 33 13688.0 34 15672.0 35 16997.5 36 17393.0 37 17624.0 38 15666.0 39 13479.0 40 13064.0 41 13147.5 42 13380.0 43 13976.0 44 14617.5 45 14251.0 46 13808.5 47 14071.0 48 15259.5 49 15507.5 50 14382.0 51 13241.5 52 12414.5 53 12129.0 54 11204.0 55 10194.5 56 9555.5 57 8786.5 58 8304.0 59 7607.5 60 6533.5 61 5380.0 62 4251.5 63 3052.5 64 2116.5 65 1671.0 66 1265.5 67 903.0 68 648.5 69 466.0 70 347.0 71 260.5 72 191.0 73 167.5 74 133.5 75 95.0 76 65.0 77 36.5 78 18.0 79 10.0 80 8.0 81 8.5 82 7.5 83 5.0 84 2.0 85 3.0 86 2.5 87 2.0 88 2.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03058053081144502 2 0.027044006840053418 3 0.023715513690508387 4 0.04847118149024959 5 0.08778900681925034 6 0.10255919517035644 7 0.05096755135240837 8 0.07343488011183737 9 0.06240924655396944 10-11 0.0650096318270515 12-13 0.06147310785565989 14-15 0.05700044518595875 16-17 0.04327041094408548 18-19 0.05450407532379997 20-21 0.055648244843956085 22-23 0.033180916084527085 24-25 0.009881464037711826 26-27 0.0028084160949286246 28-29 0.006656986299090074 30-31 0.0057208476007805315 32-33 0.00780115581924618 34-35 0.007593124997399615 36-37 0.007281078764629767 38-39 0.004160616436931296 40-41 0.006865017120936638 42-43 0.0024963698621587775 44-45 0.0023923544512354953 46-47 0.0011441695201561063 48-49 3.120462327698472E-4 50-51 0.0016642465747725185 52-53 0.005824863011703814 54-55 0.013209957187256864 56-57 0.009777448626788544 58-59 0.0062409246553969434 60-61 0.0031204623276984717 62-63 0.00915335616124885 64-65 0.002912431505851907 66-67 0.020178989719116785 68-69 0.0443105650533183 70-71 0.05252778251625761 72-73 0.004056601026008013 74-75 0.013209957187256864 76-77 0.02132315923927289 78-79 0.026627945196360296 80-81 0.0756192037412263 82-83 0.02746006848374655 84-85 0.012065787667100758 86-87 0.026835976018206857 88-89 0.04295836471131563 90-91 0.03983790238361715 92-93 0.03994191779454044 94-95 0.011857756845254194 96-97 0.01612238869310877 98-99 0.035989332179455706 100-101 0.07437101881014692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 480698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.67289459880786 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8043244827795 46.36087859139239 2 10.997116137841813 12.464768075415158 3 3.1982351178749044 5.437597252125908 4 1.2956514124137881 2.937132637300925 5 0.6483164264673111 1.8370984251928841 6 0.40319687605610866 1.3710200435577864 7 0.24277728997696485 0.9631224236094116 8 0.17483426151711565 0.7926690940175921 9 0.13267331523059508 0.6767082729124132 >10 0.9094694174527147 10.342457088117309 >50 0.10993949329122195 4.320153099313236 >100 0.07868579113196125 8.302103971673175 >500 0.003309215514895567 1.0723281804731346 >1k 0.0011030718382985221 1.7981189019243449 >5k 3.6769061276617406E-4 1.323843942974315 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6353 1.321619811191226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.976080616104082 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2890 0.6012090751365723 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.21780827047335333 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 676 0.1406288355682778 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.13105941776333582 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 623 0.12960320201040987 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 569 0.11836953763069537 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 559 0.1162892294122297 No Hit GTATAGTAATGTTCTTTTATAAGAAAATGTAGCCCATTTCTTCCCATTTC 540 0.11233664379714499 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 519 0.10796799653836713 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 517 0.107551934894674 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.10671981160728773 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 499 0.10380738010143584 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 497 0.10339131845774269 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 481 0.10006282530819766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.20077054616412E-4 0.0 22-23 0.0 0.0 0.0 0.0011441695201561063 0.0 24-25 0.0 0.0 0.0 0.003224477738621754 0.0 26-27 0.0 0.0 0.0 0.004368647258777861 0.0 28-29 0.0 0.0 0.0 0.00863327910663244 0.0 30-31 0.0 0.0 0.0 0.015810342460338925 0.0 32-33 0.0 0.0 0.0 0.021947251704812584 0.0 34-35 0.0 0.0 0.0 0.02870825341482594 0.0 36-37 0.0 0.0 0.0 0.03557327053576258 0.0 38-39 0.0 0.0 0.0 0.04337442635500876 0.0 40-41 0.0 0.0 0.0 0.052527782516257615 0.0 42-43 0.0 0.0 0.0 0.0743710188101469 0.0 44-45 0.0 0.0 0.0 0.10984027393498622 0.0 46-47 0.0 0.0 0.0 0.1406288355682778 0.0 48-49 0.0 0.0 0.0 0.20480634410794302 0.0 50-51 0.0 0.0 0.0 0.26846377559299184 0.0 52-53 0.0 0.0 0.0 0.33680190056958836 0.0 54-55 0.0 0.0 0.0 0.4726460272353952 0.0 56-57 0.0 0.0 0.0 0.6001689210273393 0.0 58-59 0.0 0.0 0.0 0.7658654706281283 0.0 60-61 0.0 0.0 0.0 0.9479964551547957 0.0 62-63 0.0 0.0 0.0 1.1837993917178768 0.0 64-65 0.0 0.0 0.0 1.365826360833621 0.0 66-67 0.0 0.0 0.0 1.6207681330065862 0.0 68-69 0.0 0.0 0.0 1.947688569538463 0.0 70-71 0.0 0.0 0.0 2.1470861122783953 0.0 72-73 0.0 0.0 0.0 2.459548406691935 0.0 74-75 0.0 0.0 0.0 2.8489821051887048 0.0 76-77 0.0 0.0 0.0 3.1432417026906707 0.0 78-79 0.0 0.0 0.0 3.391318457742699 0.0 80-81 0.0 0.0 0.0 3.7763835089806905 0.0 82-83 0.0 0.0 0.0 4.249237567037932 0.0 84-85 0.0 0.0 0.0 4.811544878489197 0.0 86-87 0.0 0.0 0.0 5.6531335682694746 0.0 88-89 0.0 0.0 0.0 6.516877540576411 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGTG 25 0.0050151055 57.01262 7 GGTATCA 2350 0.0 53.80587 1 TGCACCG 50 2.808694E-5 47.545166 5 GTATCAA 4490 0.0 46.15896 1 TATCAAC 5710 0.0 36.383667 2 ATCAACG 5825 0.0 35.583744 3 TCAACGC 5860 0.0 35.3749 4 CAACGCA 5990 0.0 34.52779 5 TATAGGA 125 5.5915734E-9 34.22895 2 AACGCAG 6105 0.0 33.866806 6 GTATAGG 75 3.0624392E-4 31.690176 1 GTCGGTA 30 0.0039473595 31.67203 90-91 GTGTAGG 125 2.1355663E-7 30.422565 1 ACGCAGA 6860 0.0 29.919159 7 CGCAGAG 6910 0.0 29.702667 8 GCAGAGT 7095 0.0 28.252663 9 TCGGTAC 35 0.00838397 27.147453 90-91 TGGTATC 940 0.0 26.298841 2 GTGGTAT 940 0.0 26.2961 1 AGGACCG 110 9.404395E-5 25.933727 5 >>END_MODULE