FastQCFastQC Report
Thu 26 May 2016
SRR937036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480698
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48451.0079093318466064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.8144406675293011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.5115477909207028No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.1984614040416228No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA8430.1753699828166541No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7410.1541508389883045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.13064335611964267No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5860.12190606160208696No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG5680.11816150680884879No Hit
ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT5610.11670529105592285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.11670529105592285No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT5100.10609571914174803No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.10276722599220299No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4890.10172707188297017No Hit
ATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCAAAA4860.1011029794174305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.046.6280821
GTATCAA47700.040.5360531
TAGACCG550.00257867934.543984
TATCAAC61600.031.3825872
ATCAACG62800.030.6316553
TCAACGC62950.030.5586644
CAACGCA63550.030.2701475
AACGCAG65000.029.597976
GTATAGA1205.366228E-627.7129141
ACGCAGA72550.026.2559197
CGCAGAG72300.026.1495978
GCAGAGT74600.024.897649
ACTATAC3000.023.7489853
GAGTACT45050.023.6184212-13
GTACATG38100.023.3175051
TACATGG37950.023.1544782
GTATAGC1852.5312147E-723.111931
AGTACTT45650.022.89177912-13
ACATGGG38200.022.8786033
ATAGCCC1503.0294766E-522.165723
Produced by FastQC (version 0.11.5)