##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937036_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480698 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.133397684200894 33.0 31.0 34.0 31.0 34.0 2 32.24745474289471 33.0 31.0 34.0 30.0 34.0 3 32.33895293926748 34.0 31.0 34.0 30.0 34.0 4 36.046894307860654 37.0 35.0 37.0 35.0 37.0 5 35.91087751561271 37.0 35.0 37.0 35.0 37.0 6 36.01792809622674 37.0 35.0 37.0 35.0 37.0 7 35.975722803090505 37.0 35.0 37.0 35.0 37.0 8 35.9326978685162 37.0 35.0 37.0 35.0 37.0 9 37.605078448422915 39.0 37.0 39.0 35.0 39.0 10-11 37.53175070418433 39.0 37.0 39.0 35.0 39.0 12-13 37.42525972648107 39.0 37.0 39.0 34.5 39.0 14-15 38.54799375075412 40.0 38.0 41.0 34.0 41.0 16-17 38.5679210231788 40.0 38.0 41.0 34.5 41.0 18-19 38.75327128467354 40.0 38.0 41.0 35.0 41.0 20-21 38.781219185434516 40.0 38.0 41.0 35.0 41.0 22-23 38.6651910347037 40.0 38.0 41.0 34.0 41.0 24-25 38.561404457684446 40.0 38.0 41.0 34.0 41.0 26-27 38.331879891324704 40.0 38.0 41.0 34.0 41.0 28-29 38.13614161074105 40.0 37.5 41.0 34.0 41.0 30-31 37.97661733562444 40.0 37.0 41.0 34.0 41.0 32-33 37.68275611714631 40.0 36.5 41.0 33.0 41.0 34-35 37.461437326554304 40.0 36.0 41.0 33.0 41.0 36-37 37.24820573416157 40.0 35.5 41.0 33.0 41.0 38-39 36.96933729701392 39.0 35.0 41.0 32.0 41.0 40-41 36.51503854811129 39.0 35.0 41.0 30.5 41.0 42-43 36.16337388547487 39.0 35.0 41.0 30.0 41.0 44-45 35.687346317230364 39.0 35.0 41.0 29.0 41.0 46-47 34.951783656266514 39.0 35.0 40.0 25.0 41.0 48-49 34.30086457609559 39.0 34.5 40.0 19.5 41.0 50-51 33.89912377417838 38.0 34.0 40.0 13.5 41.0 52-53 33.6022710724821 38.0 34.0 40.0 8.5 41.0 54-55 33.19397209890617 38.0 33.0 40.0 6.5 41.0 56-57 32.956139821675976 38.0 33.0 40.0 2.0 41.0 58-59 32.62453869165256 37.0 33.0 40.0 2.0 41.0 60-61 32.25607033938148 37.0 32.0 40.0 2.0 41.0 62-63 31.881003249441434 36.0 31.5 39.0 2.0 40.0 64-65 31.55347016213922 36.0 31.0 39.0 2.0 40.0 66-67 31.234732617984683 35.0 31.0 39.0 2.0 40.0 68-69 30.7590805453736 35.0 30.5 38.0 2.0 40.0 70-71 30.33117570699275 35.0 30.0 37.5 2.0 39.5 72-73 29.932320292574545 35.0 29.5 37.0 2.0 39.0 74-75 29.458543617822418 34.0 29.0 36.5 2.0 39.0 76-77 28.06284299081752 32.5 27.0 35.0 2.0 37.0 78-79 28.70844584333615 34.0 29.0 35.0 2.0 37.0 80-81 28.559359514705697 34.0 29.0 35.0 2.0 37.0 82-83 28.239792967726096 34.0 28.5 35.0 2.0 36.5 84-85 27.84183000553362 34.0 27.5 35.0 2.0 36.0 86-87 27.50132723664338 33.5 27.0 35.0 2.0 36.0 88-89 27.134872622727784 33.0 26.0 35.0 2.0 35.0 90-91 26.72574672663502 33.0 25.5 35.0 2.0 35.0 92-93 26.260720868403865 33.0 25.0 35.0 2.0 35.0 94-95 25.771347082783784 32.0 23.5 34.0 2.0 35.0 96-97 25.065781426176102 32.0 19.5 34.0 2.0 35.0 98-99 24.303687554348052 32.0 8.5 34.0 2.0 35.0 100-101 21.50067714032511 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16434459722189132 1101 2 0.2646202388870975 1101 3 0.2721969388861609 1101 4 0.09200519423168885 1101 5 0.02831558160166736 1101 6 0.11295457879133153 1101 7 0.22741264733887334 1101 8 0.19840844792886259 1101 9 0.14442492726718825 1101 10-11 0.20339597079993865 1101 12-13 0.10268961613704874 1101 14-15 0.27614873701202214 1101 16-17 0.38446821036581724 1101 18-19 0.22797135412562142 1101 20-21 0.08525318618116984 1101 22-23 0.1735036536937713 1101 24-25 0.053945079351102265 1101 26-27 0.2983303533311741 1101 28-29 0.2549162120471493 1101 30-31 0.13764684960493412 1101 32-33 0.4146346447473803 1101 34-35 0.44480716788201846 1101 36-37 0.21303339255855036 1101 38-39 0.3336083832729031 1101 40-41 0.5568345493037654 1101 42-43 0.6208982898115494 1101 44-45 0.4475408119881976 1101 46-47 0.5317736793495769 1101 48-49 0.39804475340886825 1101 50-51 0.4887888684126409 1101 52-53 0.5847074016132083 1101 54-55 0.48722367419082957 1101 56-57 0.5871417293314352 1101 58-59 0.583860716741782 1101 60-61 0.659635637339413 1101 62-63 0.7071316081512364 1101 64-65 0.8158736226622416 1101 66-67 0.7101085521503876 1101 68-69 0.1385497222107155 1101 70-71 0.4292641933762589 1101 72-73 0.5238035550858768 1101 74-75 0.519589392250964 1101 76-77 0.4966406053280039 1101 78-79 0.6412064482480382 1101 80-81 0.6737939753296551 1101 82-83 0.9150267599295603 1101 84-85 0.6735573638018941 1101 86-87 0.37800795665376796 1101 88-89 0.31958413955886655 1101 90-91 0.6219448488080666 1101 92-93 0.638984961702473 1101 94-95 0.3729477399592618 1101 96-97 0.3100508310232577 1101 98-99 0.41664235780745074 1101 100-101 0.9582920814882847 1102 1 -0.014069528336563053 1102 2 0.006436833260281105 1102 3 0.08913311937527624 1102 4 0.13463450978112945 1102 5 -0.13874831221514938 1102 6 0.024993069266237455 1102 7 0.16158501853417562 1102 8 0.06392956474707745 1102 9 0.2213074217174622 1102 10-11 0.303651397455333 1102 12-13 0.37855597691094545 1102 14-15 0.18578528810199657 1102 16-17 0.02708730884904753 1102 18-19 0.16091767737501783 1102 20-21 0.11212128385631814 1102 22-23 -0.03755492724860687 1102 24-25 0.01432794068694676 1102 26-27 -0.12842164131880196 1102 28-29 -0.22016250580391983 1102 30-31 -0.1183863279955446 1102 32-33 -0.17691862796468882 1102 34-35 -0.29133431062034276 1102 36-37 -0.18346029608100167 1102 38-39 -0.2550460291064667 1102 40-41 -0.1701412919787515 1102 42-43 -0.25122398060633344 1102 44-45 -0.3167561145325166 1102 46-47 -0.2386488427796607 1102 48-49 -0.05753144268449262 1102 50-51 0.02909705228267967 1102 52-53 0.04205672310713027 1102 54-55 0.2484499079015734 1102 56-57 0.15991510551452848 1102 58-59 -0.07854976782579826 1102 60-61 0.13098977014269764 1102 62-63 -0.08147357654545573 1102 64-65 0.17720295585934664 1102 66-67 0.12879092213822219 1102 68-69 0.07308526366878354 1102 70-71 -0.1371829611707973 1102 72-73 0.059639450425965634 1102 74-75 -0.1512501719447208 1102 76-77 -0.33594904942803794 1102 78-79 -0.5811457061596954 1102 80-81 -0.33047490070017815 1102 82-83 -0.24072219746527423 1102 84-85 -0.19534838840638713 1102 86-87 -0.2705528302460962 1102 88-89 -0.2625462116295658 1102 90-91 -0.1408863931227735 1102 92-93 -0.30546763575440394 1102 94-95 -0.3280303119248593 1102 96-97 0.25378080325141283 1102 98-99 0.028777445999310203 1102 100-101 0.27739335119096964 1103 1 -0.0013361347704687887 1103 2 0.18212667612521471 1103 3 0.11928236649929858 1103 4 0.03901936090627345 1103 5 -0.05157648183006813 1103 6 9.247822175595388E-4 1103 7 0.06493960772729679 1103 8 0.11474240519130063 1103 9 0.22645671914723664 1103 10-11 0.1443320544887854 1103 12-13 0.10942520004917355 1103 14-15 0.09964774410960331 1103 16-17 -0.001954205475016124 1103 18-19 -0.1685558628021795 1103 20-21 -0.06272442838266556 1103 22-23 0.09445341948282504 1103 24-25 0.06486510557513014 1103 26-27 0.05624174272944771 1103 28-29 -0.05899392315860297 1103 30-31 0.0911210578959043 1103 32-33 0.034874651588431504 1103 34-35 0.2595781352255031 1103 36-37 0.15162736673358523 1103 38-39 -0.034917085386538815 1103 40-41 -0.0012155613882214311 1103 42-43 -0.37034801649647164 1103 44-45 -0.37632115082309525 1103 46-47 -0.2651468979590419 1103 48-49 -0.2607274681271363 1103 50-51 -0.48784894549179114 1103 52-53 -0.6182637044515715 1103 54-55 -0.413071821167712 1103 56-57 -0.4918201413157135 1103 58-59 -0.6160205150272944 1103 60-61 -1.1096347456643763 1103 62-63 -0.7844301465781172 1103 64-65 -0.9711587868126124 1103 66-67 -0.9571323193233816 1103 68-69 -1.0456826074518872 1103 70-71 -0.9751853396270569 1103 72-73 -0.5791561492287371 1103 74-75 -0.8293174649516182 1103 76-77 -0.6287919485690168 1103 78-79 -0.5800530650872595 1103 80-81 -0.5624065814558108 1103 82-83 -0.1174881811138917 1103 84-85 -0.8796888094857067 1103 86-87 -1.1434985946018479 1103 88-89 -0.7988287368176579 1103 90-91 -0.7822405436496744 1103 92-93 -0.7585313570524512 1103 94-95 -0.6147630736506287 1103 96-97 -0.41998232906681565 1103 98-99 -0.33698767440249355 1103 100-101 0.3564991562742641 1104 1 0.056435266472952605 1104 2 0.07662691524525655 1104 3 -0.029832889359141745 1104 4 -0.045150892560968714 1104 5 -0.20863052295871398 1104 6 -0.08496713552514734 1104 7 0.17302186502045913 1104 8 -0.032938130437585755 1104 9 0.01010090853031187 1104 10-11 0.0927299502324459 1104 12-13 0.10129511874836794 1104 14-15 0.12504229217273632 1104 16-17 0.005267219680419544 1104 18-19 0.1029888526449767 1104 20-21 -0.024465222261191855 1104 22-23 -0.08928641791554526 1104 24-25 0.11005879280612163 1104 26-27 0.03180367481935065 1104 28-29 -0.04254246452637034 1104 30-31 -0.1333648340218403 1104 32-33 -0.1638341002049728 1104 34-35 -0.17887236692657638 1104 36-37 -0.3154218919942906 1104 38-39 -0.005744440718913779 1104 40-41 -0.1791208698530724 1104 42-43 -0.3269238175485967 1104 44-45 0.06437707968861872 1104 46-47 -0.22573991565392504 1104 48-49 -0.20859929978662706 1104 50-51 -0.14351609039853486 1104 52-53 -0.23973668388725144 1104 54-55 -0.15123537927387787 1104 56-57 -0.2099454402157619 1104 58-59 -0.34079335099094266 1104 60-61 -0.3741015079790593 1104 62-63 -0.4762044172619966 1104 64-65 -0.541699198099078 1104 66-67 -0.6244685220971746 1104 68-69 -0.41416658640932624 1104 70-71 -0.4684421545481463 1104 72-73 -0.23797967864049951 1104 74-75 -0.34089087480336033 1104 76-77 -0.039600174298335844 1104 78-79 -0.05221949263388481 1104 80-81 -0.01506081388048841 1104 82-83 0.1475524692926129 1104 84-85 0.01811128616230917 1104 86-87 -0.17903183228716912 1104 88-89 0.021161698380812055 1104 90-91 -0.04092060103848283 1104 92-93 -0.04499716288698252 1104 94-95 -0.249531127213519 1104 96-97 -0.3399249402576352 1104 98-99 -0.36572990300124886 1104 100-101 0.27032029448564643 1105 1 -0.08410018317455936 1105 2 0.023838172707058902 1105 3 -0.048242606676076605 1105 4 -0.0024091022810566187 1105 5 -0.22971887353557463 1105 6 -0.06398958673773336 1105 7 0.07623650193684028 1105 8 0.056102187563546124 1105 9 -0.0799892564258542 1105 10-11 -0.013932007358164356 1105 12-13 0.07248665349165151 1105 14-15 0.07220230380691817 1105 16-17 -0.08529385288090907 1105 18-19 -0.12592487431171406 1105 20-21 0.040326124119054896 1105 22-23 0.15316451766706507 1105 24-25 -0.066136827753148 1105 26-27 -0.2878650278302004 1105 28-29 -0.3982771496415296 1105 30-31 -0.26730480715240645 1105 32-33 -0.3232016989916886 1105 34-35 -0.3396485334616628 1105 36-37 -0.3138831321433244 1105 38-39 -0.6840106697185178 1105 40-41 -0.636240577336352 1105 42-43 -0.6002136611795734 1105 44-45 -0.5163004732538568 1105 46-47 -0.5606369392408581 1105 48-49 -0.5777700868948443 1105 50-51 -0.5547223063789275 1105 52-53 -0.5526686377383072 1105 54-55 -0.378164369177064 1105 56-57 -0.5626884770157972 1105 58-59 -0.4905765949903369 1105 60-61 -0.5383166850541699 1105 62-63 -0.5247286759287775 1105 64-65 -0.25225029849935154 1105 66-67 -0.3212677354044686 1105 68-69 -0.3149505090696749 1105 70-71 -0.6584824586990585 1105 72-73 -0.5235491305871491 1105 74-75 -0.4646260031256837 1105 76-77 -0.5954304259568985 1105 78-79 -0.3104943087721672 1105 80-81 -0.3878326591457899 1105 82-83 -0.47542643681672203 1105 84-85 -0.6758791017824564 1105 86-87 -0.4102294611288748 1105 88-89 -0.3418055735406291 1105 90-91 -0.4599882039802736 1105 92-93 -0.4795479308818855 1105 94-95 -0.5677497114436534 1105 96-97 -0.270838639513876 1105 98-99 0.048344694303498414 1105 100-101 0.4635336848819023 1106 1 0.039791513396593814 1106 2 0.12030191566957882 1106 3 0.05563649978151375 1106 4 0.08966588358173766 1106 5 -0.10331626731093735 1106 6 0.029756967874547513 1106 7 0.1280942549927886 1106 8 0.09413215487904836 1106 9 -0.003922208736355515 1106 10-11 0.08370539025825252 1106 12-13 0.2820409136802198 1106 14-15 0.3044098770803174 1106 16-17 0.17484136636133485 1106 18-19 0.1528963496322575 1106 20-21 0.1296074562526499 1106 22-23 0.22083459978732378 1106 24-25 0.25186845724932283 1106 26-27 0.4108827182193906 1106 28-29 0.08219572648810924 1106 30-31 0.08206195281388062 1106 32-33 0.18908610958168737 1106 34-35 0.2766090730652806 1106 36-37 0.1731586535188825 1106 38-39 0.4949005344155921 1106 40-41 0.5449485837850929 1106 42-43 0.8671356220019604 1106 44-45 0.0856527726125691 1106 46-47 0.4256544187487066 1106 48-49 0.4748452068922546 1106 50-51 0.49923557720164524 1106 52-53 0.2787554321403789 1106 54-55 0.7176734246367076 1106 56-57 0.6512100836568067 1106 58-59 0.6489549389762956 1106 60-61 0.5856639247483386 1106 62-63 0.38319100341454515 1106 64-65 0.5413074044095332 1106 66-67 0.5107182660963865 1106 68-69 0.35220814414382673 1106 70-71 0.2903337505386716 1106 72-73 0.29068267687658533 1106 74-75 0.20608028801891365 1106 76-77 0.35206097613362886 1106 78-79 0.5123051776650094 1106 80-81 0.37182242864713544 1106 82-83 0.37439022410022105 1106 84-85 0.41353026540429383 1106 86-87 0.40380673722587446 1106 88-89 0.9191334213130737 1106 90-91 0.43380316290061316 1106 92-93 0.7864182180726722 1106 94-95 0.9799629067664881 1106 96-97 1.24889831958248 1106 98-99 1.3323317769892462 1106 100-101 1.7054985961642792 1107 1 0.27063975931514506 1107 2 0.3485346138211014 1107 3 0.2622314845875593 1107 4 0.12176578799535775 1107 5 0.21744499975551435 1107 6 0.2023117873779725 1107 7 0.10931462202547237 1107 8 0.08672777692763844 1107 9 0.21581233694299584 1107 10-11 0.3350452640461796 1107 12-13 0.2442938038605007 1107 14-15 0.3992853056734873 1107 16-17 0.14079882328802285 1107 18-19 0.23563325881568886 1107 20-21 0.40587350026042657 1107 22-23 0.20570960038176622 1107 24-25 0.33743253579184085 1107 26-27 0.2516529257036595 1107 28-29 0.24206038378002148 1107 30-31 0.14717482558888406 1107 32-33 -0.034854712461203974 1107 34-35 0.08112541072774349 1107 36-37 0.21166664996174234 1107 38-39 0.23823207649499523 1107 40-41 0.31079523659728636 1107 42-43 0.08504166201507957 1107 44-45 0.01630033468426717 1107 46-47 0.03988965635933539 1107 48-49 0.34494997146918394 1107 50-51 0.15533384570902342 1107 52-53 0.44643106617293427 1107 54-55 0.6079179070198037 1107 56-57 0.4815133445315425 1107 58-59 0.40990576192273664 1107 60-61 0.3747271306490134 1107 62-63 0.2879398870512091 1107 64-65 0.5664642183870221 1107 66-67 0.3576564107694651 1107 68-69 0.3845814921876354 1107 70-71 0.16079280294949072 1107 72-73 0.4760822136868512 1107 74-75 0.22603691748139454 1107 76-77 -0.2128783193804722 1107 78-79 0.20511147019738374 1107 80-81 0.27945554813962303 1107 82-83 0.15136008823440505 1107 84-85 0.18948006590933275 1107 86-87 0.5367597857656854 1107 88-89 0.19713999347127853 1107 90-91 0.11359353863594279 1107 92-93 0.39624031326910014 1107 94-95 0.5191152790683127 1107 96-97 -0.07508244900811789 1107 98-99 -0.3515031867021925 1107 100-101 0.1308199518740203 1108 1 -0.02617163667518696 1108 2 -0.026996165610299272 1108 3 0.013459381291760053 1108 4 0.06850533527919822 1108 5 -0.1637320252921981 1108 6 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-1.9910690591442552 2316 58-59 -1.819894872775393 2316 60-61 -1.7377662984731188 2316 62-63 -1.64375705432683 2316 64-65 -1.3661988854036444 2316 66-67 -1.6038723161351136 2316 68-69 -1.5271278121754257 2316 70-71 -1.7078261445418939 2316 72-73 -1.7167268122656836 2316 74-75 -1.222452267340362 2316 76-77 -1.205849913718808 2316 78-79 -1.6453037119376184 2316 80-81 -1.8268442201760884 2316 82-83 -1.809691641610037 2316 84-85 -1.6511299062496185 2316 86-87 -1.8785367311823116 2316 88-89 -2.210300673935219 2316 90-91 -2.1837338176321914 2316 92-93 -2.13351602884487 2316 94-95 -2.2234523279639866 2316 96-97 -2.348404198127138 2316 98-99 -2.595805992223678 2316 100-101 -2.810480205827048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 9.0 5 14.0 6 51.0 7 108.0 8 212.0 9 472.0 10 886.0 11 1426.0 12 2334.0 13 3062.0 14 3865.0 15 5683.0 16 7816.0 17 10958.0 18 9276.0 19 4516.0 20 3180.0 21 2998.0 22 3169.0 23 3422.0 24 3885.0 25 4388.0 26 5195.0 27 6242.0 28 7447.0 29 9252.0 30 11354.0 31 14066.0 32 17939.0 33 23635.0 34 32538.0 35 46342.0 36 70276.0 37 101999.0 38 60208.0 39 2471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06412171806919 20.811259587253893 15.340854706545759 19.783763988131156 2 16.094249616689655 22.201626442484727 39.77003459674919 21.934089344076423 3 18.661482517016992 27.374473679830913 32.79349110456505 21.17055269858705 4 12.327698471805583 19.21081427424287 38.63215574019447 29.82933151375708 5 11.843233032653197 40.107304530173415 33.64906134540492 14.400401091768464 6 27.46963790155149 39.34154084269125 18.217467099925525 14.97135415583173 7 25.532667351787207 37.64702700678602 20.057874656226208 16.762430985200563 8 27.10475184003262 33.3007418379107 19.706551722703235 19.887954599353442 9 28.108559029908655 15.78832403780344 20.97100668404421 35.1321102482437 10-11 24.31068987181141 27.87904255894553 26.981701608910374 20.828565960332682 12-13 26.21970946047861 27.076868664277576 27.44147339841779 19.261948476826017 14-15 23.03230651768235 26.912874965933465 28.50874911843088 21.546069397953303 16-17 20.04283354621821 30.212628303009375 28.27794165983632 21.466596490936098 18-19 20.007177108145495 27.421337854564744 33.388427173155534 19.183057864134224 20-21 21.643082709582394 26.316013929859743 33.50884346382522 18.53205989673264 22-23 21.11708401898109 26.486668095171723 33.085425295773355 19.31082259007383 24-25 20.411818671637228 26.67179116990537 33.732371951562 19.184018206895402 26-27 20.436635891973758 26.808411934312186 34.06473503114221 18.690217142571843 28-29 19.79704407467929 27.498593184250737 33.73372540412798 18.97063733694199 30-31 20.481674564903535 26.812780581570966 33.80625673499786 18.89928811852764 32-33 20.10056209928063 27.0604412749793 33.48620131558692 19.352795310153155 34-35 20.24119093484891 27.155199314330414 33.5767987385011 19.026811012319587 36-37 20.992531569202605 26.4446940855853 33.33915829328674 19.223616051925358 38-39 20.180231911013742 27.751615752140037 32.79584153822309 19.27231079862314 40-41 20.410154421600303 27.60002662800059 32.79009438378022 19.19972456661889 42-43 20.345934453648653 27.696287481953323 32.13857765166487 19.819200412733153 44-45 21.031193343811207 27.712241949425366 31.406784544477738 19.849780162285686 46-47 21.257941576623992 27.883723252437083 30.604662386779225 20.2536727841597 48-49 21.107661263060447 28.44866054015259 30.229510242928242 20.214167953858716 50-51 21.224688491747724 29.059541009662244 29.235851715934846 20.479918782655187 52-53 21.481768102917435 29.218258525138552 28.74457037295921 20.555402998984807 54-55 21.508514701538182 28.75193988741372 28.73456931378953 21.00497609725857 56-57 21.704257992577432 29.693090851598512 28.03466223975236 20.567988916071698 58-59 21.34937112282556 29.390698525893594 28.246008928682876 21.013921422597974 60-61 21.56472371228173 29.07597587195786 28.788677836131942 20.570622579628466 62-63 21.404417539096833 28.647642228220448 28.83102158842099 21.116918644261723 64-65 21.272503734153254 28.779295940486545 29.136172815364326 20.812027509995882 66-67 21.38546447041594 29.135444707487864 28.336398320775206 21.142692501320994 68-69 21.411809983461445 28.737349046692813 28.637285596896163 21.213555372949582 70-71 20.869765109955836 29.164352299700642 29.040364640217355 20.92551795012617 72-73 21.458060073622796 28.73109372188307 28.82886912346433 20.981977081029804 74-75 20.8631611795912 29.003068601445886 28.47547719352993 21.658293025432982 76-77 20.51710382746702 29.327236624356534 28.59829434996926 21.557365198207183 78-79 20.612532323125155 29.433442966716804 28.00706480019472 21.946959909963322 80-81 20.434528892832365 28.869948188145372 28.29066648081942 22.404856438202845 82-83 20.230124413221233 29.676105401225094 28.29030375715238 21.80346642840129 84-85 20.323231944977547 29.114591622432133 27.9707870053976 22.591389427192716 86-87 20.06126577801817 29.09427544636124 27.921904335932012 22.922554439688575 88-89 20.220187837867357 28.969554621000853 27.99905550568413 22.81120203544766 90-91 19.939627634399766 28.856929767716178 28.39508741198731 22.80835518589675 92-93 19.808486825408053 28.713766231604875 28.53985246454115 22.937894478445926 94-95 19.729643143928204 29.392050726235595 28.09820302976089 22.78010310007531 96-97 20.355652082559715 29.313108429574292 27.374663117635006 22.956576370230987 98-99 19.64785926852837 29.29995204794789 27.333085771762793 23.71910291176094 100-101 19.85262606047241 29.42985175230194 27.171914072089237 23.54560811513641 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 425.0 1 296.5 2 175.0 3 182.5 4 166.5 5 169.0 6 233.5 7 394.5 8 614.0 9 722.0 10 707.5 11 707.0 12 737.5 13 790.0 14 887.5 15 972.5 16 1048.0 17 1166.5 18 1327.5 19 1520.5 20 1737.0 21 2025.5 22 2362.0 23 2623.0 24 2856.0 25 3709.0 26 5183.5 27 6846.5 28 7507.0 29 7771.0 30 9022.0 31 10508.0 32 11984.5 33 13292.0 34 15382.5 35 16792.0 36 17635.0 37 17617.5 38 15382.0 39 13851.0 40 13615.0 41 13620.0 42 13507.5 43 14286.5 44 14976.0 45 14345.0 46 13818.0 47 14102.0 48 15364.0 49 15513.5 50 14306.5 51 13279.0 52 12613.0 53 12176.0 54 11340.0 55 10622.0 56 9997.0 57 9216.0 58 8789.5 59 8205.0 60 7108.5 61 5867.0 62 4634.5 63 3332.0 64 2241.0 65 1678.0 66 1269.0 67 955.5 68 714.5 69 506.5 70 372.5 71 281.0 72 216.0 73 197.5 74 163.0 75 136.5 76 101.0 77 50.0 78 28.0 79 16.5 80 7.5 81 3.5 82 5.0 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023299452046815255 2 0.003536523971391601 3 4.1606164369312963E-4 4 0.0 5 0.002080308218465648 6 0.0 7 8.321232873862593E-4 8 0.0 9 2.0803082184656481E-4 10-11 0.0 12-13 0.008425248284785873 14-15 0.00436864725877786 16-17 0.0 18-19 6.240924655396944E-4 20-21 8.321232873862593E-4 22-23 0.0018722773966190832 24-25 2.0803082184656481E-4 26-27 0.0 28-29 5.20077054616412E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0016642465747725185 38-39 0.008217217462939309 40-41 2.0803082184656481E-4 42-43 0.0 44-45 5.20077054616412E-4 46-47 0.0 48-49 1.0401541092328241E-4 50-51 0.0026003852730820598 52-53 4.1606164369312963E-4 54-55 0.0 56-57 4.1606164369312963E-4 58-59 0.0 60-61 0.0026003852730820598 62-63 1.0401541092328241E-4 64-65 0.0 66-67 0.0 68-69 6.240924655396944E-4 70-71 0.001040154109232824 72-73 9.361386983095416E-4 74-75 0.004784708902470991 76-77 3.120462327698472E-4 78-79 0.001040154109232824 80-81 0.003432508560468319 82-83 3.120462327698472E-4 84-85 0.004472662669701143 86-87 0.0011441695201561063 88-89 0.0036405393823148837 90-91 0.0030164469167751895 92-93 0.0 94-95 0.0 96-97 7.281078764629767E-4 98-99 0.0019762928075423656 100-101 0.003328493149545037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 480698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.47741229737415 #Duplication Level Percentage of deduplicated Percentage of total 1 80.52047044610366 44.67067337317023 2 11.513553873731762 12.774843505220925 3 3.464296706793128 5.765706501695936 4 1.4809816193544976 3.286441116070492 5 0.7485045833096676 2.0762548687372333 6 0.46313097449618135 1.5415984811885597 7 0.31880358599926717 1.2380478587653907 8 0.18226824714047776 0.8089416556265577 9 0.13600441664660332 0.6790655786910721 >10 0.9713675936419266 10.732277460722305 >50 0.11409909935014696 4.387139524382732 >100 0.08198511438823038 8.439966898557795 >500 0.0034003042833828266 1.2475397466962028 >1k 0.0011334347611276089 2.3515034304745797 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4845 1.0079093318466064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3915 0.8144406675293011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.5115477909207028 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.1984614040416228 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 843 0.1753699828166541 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 741 0.1541508389883045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 628 0.13064335611964267 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 586 0.12190606160208696 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 568 0.11816150680884879 No Hit ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT 561 0.11670529105592285 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.11670529105592285 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 510 0.10609571914174803 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 0.10276722599220299 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 489 0.10172707188297017 No Hit ATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCAAAA 486 0.1011029794174305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.080308218465648E-4 0.0 0.0 0.0 0.0 9 2.080308218465648E-4 0.0 0.0 0.0 0.0 10-11 2.080308218465648E-4 0.0 0.0 0.0 0.0 12-13 2.080308218465648E-4 0.0 0.0 0.0 0.0 14-15 2.080308218465648E-4 0.0 0.0 0.0 0.0 16-17 2.080308218465648E-4 0.0 0.0 0.0 0.0 18-19 2.080308218465648E-4 0.0 0.0 0.0 0.0 20-21 2.080308218465648E-4 1.040154109232824E-4 0.0 5.20077054616412E-4 0.0 22-23 2.080308218465648E-4 2.080308218465648E-4 0.0 0.001352200342002671 0.0 24-25 2.080308218465648E-4 2.080308218465648E-4 0.0 0.003224477738621754 0.0 26-27 2.080308218465648E-4 2.080308218465648E-4 0.0 0.004264631847854578 0.0 28-29 2.080308218465648E-4 2.080308218465648E-4 0.0 0.00863327910663244 0.0 30-31 2.080308218465648E-4 2.080308218465648E-4 0.0 0.015810342460338925 0.0 32-33 2.080308218465648E-4 2.080308218465648E-4 0.0 0.021947251704812584 0.0 34-35 2.080308218465648E-4 2.080308218465648E-4 0.0 0.028916284236672505 0.0 36-37 2.080308218465648E-4 2.080308218465648E-4 0.0 0.03557327053576258 0.0 38-39 2.080308218465648E-4 2.080308218465648E-4 0.0 0.04379048799870189 0.0 40-41 2.080308218465648E-4 2.080308218465648E-4 0.0 0.053671952036413714 0.0 42-43 2.080308218465648E-4 2.080308218465648E-4 0.0 0.07530715750845646 0.0 44-45 2.080308218465648E-4 2.080308218465648E-4 0.0 0.11160853592068201 0.0 46-47 3.120462327698472E-4 2.080308218465648E-4 0.0 0.14166898967751063 0.0 48-49 4.160616436931296E-4 2.080308218465648E-4 0.0 0.2056384673953293 0.0 50-51 4.160616436931296E-4 2.080308218465648E-4 0.0 0.2690878680585316 0.0 52-53 4.160616436931296E-4 2.080308218465648E-4 0.0 0.3359697772822021 0.0 54-55 4.160616436931296E-4 2.080308218465648E-4 0.0 0.4731661042900116 0.0 56-57 4.160616436931296E-4 2.080308218465648E-4 0.0 0.6032893833550379 0.0 58-59 4.160616436931296E-4 2.080308218465648E-4 0.0 0.7703381332978294 0.0 60-61 4.160616436931296E-4 2.080308218465648E-4 0.0 0.9527811640572668 0.0 62-63 4.160616436931296E-4 2.080308218465648E-4 0.0 1.1905603934278903 0.0 64-65 4.160616436931296E-4 2.080308218465648E-4 0.0 1.3737315320637906 0.0 66-67 4.160616436931296E-4 2.080308218465648E-4 0.0 1.6296094429350654 0.0 68-69 4.160616436931296E-4 2.080308218465648E-4 0.0 1.9572579873434046 0.0 70-71 4.160616436931296E-4 2.080308218465648E-4 0.0 2.1567595454942605 0.0 72-73 4.160616436931296E-4 2.080308218465648E-4 0.0 2.470261994017034 0.0 74-75 4.160616436931296E-4 2.080308218465648E-4 0.0 2.8643763860053504 0.0 76-77 4.160616436931296E-4 2.080308218465648E-4 0.0 3.161444399602245 0.0 78-79 4.160616436931296E-4 2.080308218465648E-4 0.0 3.4113934320508923 0.0 80-81 4.160616436931296E-4 2.080308218465648E-4 0.0 3.7967705295216536 0.0 82-83 4.160616436931296E-4 2.080308218465648E-4 0.0 4.271808911208285 0.0 84-85 4.160616436931296E-4 2.080308218465648E-4 0.0 4.834740315125089 0.0 86-87 4.160616436931296E-4 2.080308218465648E-4 0.0 5.675704912439826 0.0 88-89 4.160616436931296E-4 2.080308218465648E-4 0.0 6.539448884746764 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2700 0.0 46.628082 1 GTATCAA 4770 0.0 40.536053 1 TAGACCG 55 0.002578679 34.54398 4 TATCAAC 6160 0.0 31.382587 2 ATCAACG 6280 0.0 30.631655 3 TCAACGC 6295 0.0 30.558664 4 CAACGCA 6355 0.0 30.270147 5 AACGCAG 6500 0.0 29.59797 6 GTATAGA 120 5.366228E-6 27.712914 1 ACGCAGA 7255 0.0 26.255919 7 CGCAGAG 7230 0.0 26.149597 8 GCAGAGT 7460 0.0 24.89764 9 ACTATAC 300 0.0 23.748985 3 GAGTACT 4505 0.0 23.61842 12-13 GTACATG 3810 0.0 23.317505 1 TACATGG 3795 0.0 23.154478 2 GTATAGC 185 2.5312147E-7 23.11193 1 AGTACTT 4565 0.0 22.891779 12-13 ACATGGG 3820 0.0 22.878603 3 ATAGCCC 150 3.0294766E-5 22.16572 3 >>END_MODULE