FastQCFastQC Report
Thu 26 May 2016
SRR937035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256459
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79040.3502833421746196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59000.2614716243459332No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41920.18577780495900878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36820.16317602048164848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29490.13069149494850116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140250.027.4179821
GGTATCA101000.025.1780571
ACCGCGC1401.7735283E-523.7619848
GTACATG104400.023.2198541
TATCAAC163750.023.1934282
ATCAACG162600.023.1497483
TCAACGC163000.023.0929374
CAACGCA164550.022.9042975
AACGCAG167150.022.6333226
GAGTACT69400.022.28475412-13
TACATGG106850.022.1096212
ACATGGG104600.021.8097743
ACGCAGA182550.020.4371247
CGCAGAG182400.020.270648
GTACTTT76100.020.22977814-15
AGAGTAC128750.020.07579610-11
AGTACTT75950.019.6747512-13
GCAGAGT189100.018.7984819
CATGGGA63550.018.0236664
CATGGGG47750.017.5178554
Produced by FastQC (version 0.11.5)