##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256459 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.736889524693336 33.0 31.0 34.0 30.0 34.0 2 31.840035205603115 33.0 31.0 34.0 30.0 34.0 3 31.852530890213384 33.0 31.0 34.0 30.0 34.0 4 35.413864820942905 37.0 35.0 37.0 33.0 37.0 5 35.44221011771098 37.0 35.0 37.0 33.0 37.0 6 35.49486030989262 37.0 35.0 37.0 33.0 37.0 7 35.43619228180082 37.0 35.0 37.0 33.0 37.0 8 35.40181408126627 37.0 35.0 37.0 33.0 37.0 9 37.01887780810553 39.0 37.0 39.0 33.0 39.0 10-11 36.888093468571775 39.0 37.0 39.0 33.0 39.0 12-13 36.78056370623176 39.0 37.0 39.0 33.0 39.0 14-15 37.93802191841287 40.0 38.0 41.0 33.0 41.0 16-17 37.8721915620891 40.0 38.0 41.0 33.0 41.0 18-19 37.82687387628138 40.0 38.0 41.0 32.5 41.0 20-21 37.755617762166295 40.0 37.5 41.0 32.0 41.0 22-23 37.619525991830564 40.0 37.0 41.0 32.0 41.0 24-25 37.448764856795535 40.0 37.0 41.0 32.0 41.0 26-27 37.418583719003976 40.0 37.0 41.0 32.0 41.0 28-29 37.49863990438116 40.0 37.0 41.0 32.0 41.0 30-31 37.37607929060532 40.0 37.0 41.0 31.5 41.0 32-33 37.208604942522776 40.0 36.5 41.0 31.0 41.0 34-35 37.02056540801318 39.5 36.0 41.0 31.0 41.0 36-37 36.881028416647496 39.0 36.0 41.0 30.0 41.0 38-39 36.73495020295073 39.0 36.0 41.0 30.0 41.0 40-41 36.54253855266149 39.0 35.5 41.0 30.0 41.0 42-43 36.33874180740709 39.0 35.0 41.0 30.0 41.0 44-45 36.10487759804189 39.0 35.0 40.5 29.5 41.0 46-47 35.77971414503875 39.0 35.0 40.0 28.0 41.0 48-49 35.46172941764065 39.0 35.0 40.0 27.0 41.0 50-51 34.81406907903046 38.5 34.0 39.5 26.0 40.5 52-53 35.135005333577965 38.5 34.5 40.0 27.0 40.5 54-55 35.45289367101286 39.0 35.0 40.5 27.0 41.0 56-57 35.333122826517126 39.0 35.0 41.0 26.5 41.0 58-59 35.0987234866665 38.5 34.5 40.0 26.0 41.0 60-61 34.806649710896586 38.0 34.0 40.0 26.0 41.0 62-63 34.50748252017874 38.0 34.0 40.0 26.0 41.0 64-65 34.19394724211696 37.0 34.0 40.0 25.0 41.0 66-67 33.822053934948514 37.0 33.5 39.5 24.5 41.0 68-69 33.37790205804758 36.0 33.0 39.0 23.0 41.0 70-71 33.01492825706117 36.0 33.0 39.0 22.0 40.5 72-73 32.63283467592365 35.0 32.0 38.5 22.0 40.0 74-75 32.203144838882515 35.0 32.0 37.5 20.5 39.5 76-77 31.765797650212125 35.0 31.5 37.0 20.0 39.0 78-79 31.34019297492221 35.0 31.0 36.5 18.0 39.0 80-81 30.915293165087423 35.0 31.0 36.0 17.5 37.5 82-83 30.511609118534835 34.5 31.0 35.5 12.5 37.0 84-85 30.169442254434934 34.0 30.0 35.0 8.5 37.0 86-87 29.856724629164546 34.0 30.0 35.0 7.0 36.0 88-89 29.491803972507366 34.0 30.0 35.0 2.0 36.0 90-91 29.15977866205413 34.0 29.0 35.0 2.0 36.0 92-93 28.809445906174233 34.0 29.0 35.0 2.0 35.0 94-95 28.423197806829194 34.0 29.0 35.0 2.0 35.0 96-97 27.994869173337516 34.0 29.0 35.0 2.0 35.0 98-99 27.413271191721186 34.0 27.5 35.0 2.0 35.0 100-101 25.26836915716173 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4034968421735563 1101 2 0.29460286289814164 1101 3 0.4394318395849659 1101 4 0.33271184651344754 1101 5 0.3446894643733174 1101 6 0.22000772451567485 1101 7 0.27120221204874895 1101 8 0.2655046008799147 1101 9 0.34887123119234786 1101 10-11 0.2865328974280317 1101 12-13 0.4268955228493283 1101 14-15 0.69875984362411 1101 16-17 0.6365072243189118 1101 18-19 0.6412019490187291 1101 20-21 0.5977075744253781 1101 22-23 0.6863320254639547 1101 24-25 0.759698003376343 1101 26-27 0.8799580843470736 1101 28-29 0.7641098037091467 1101 30-31 0.7447388872303904 1101 32-33 0.7010007250752963 1101 34-35 0.6816114162062092 1101 36-37 0.6893855478853368 1101 38-39 0.8368257672990893 1101 40-41 0.8681422675168946 1101 42-43 0.9389069307628759 1101 44-45 0.8292607922379887 1101 46-47 0.9185679942887006 1101 48-49 0.9172203548692082 1101 50-51 0.8210500859682242 1101 52-53 0.949032981700725 1101 54-55 1.0314690692447073 1101 56-57 1.0447327124410606 1101 58-59 1.0155141074527378 1101 60-61 1.0832992640634984 1101 62-63 1.0876485842364119 1101 64-65 1.0359560071736595 1101 66-67 0.8723603840678109 1101 68-69 0.8556420316258055 1101 70-71 0.9638181783221995 1101 72-73 0.9479948165440177 1101 74-75 0.8507106178529114 1101 76-77 0.9069886969195515 1101 78-79 0.9681105821114393 1101 80-81 0.9111674852969749 1101 82-83 0.762146582668791 1101 84-85 0.9535073010859314 1101 86-87 1.0728785454362573 1101 88-89 1.1055967072133122 1101 90-91 0.9901751585218754 1101 92-93 0.8000392229153128 1101 94-95 0.9471210903204259 1101 96-97 0.8803507387996028 1101 98-99 0.9623878334677691 1101 100-101 1.0386478452966905 1102 1 0.3857677341595469 1102 2 0.3863775332791768 1102 3 0.36141026525817566 1102 4 0.27486247593254376 1102 5 0.24047903208086296 1102 6 0.24526054655904517 1102 7 0.32056647995616316 1102 8 0.2330885435740342 1102 9 0.22587359252135997 1102 10-11 0.36499411481536725 1102 12-13 0.3356843209086904 1102 14-15 0.41900137279284166 1102 16-17 0.4774627163564773 1102 18-19 0.38028046656617676 1102 20-21 0.44110834487948836 1102 22-23 0.49635954743854427 1102 24-25 0.5735472382949922 1102 26-27 0.4761477381531378 1102 28-29 0.5276918108656616 1102 30-31 0.49546521752632344 1102 32-33 0.530385637821631 1102 34-35 0.3701147795553794 1102 36-37 0.45723445170752086 1102 38-39 0.49033800913786507 1102 40-41 0.47108539951491935 1102 42-43 0.4346473376958855 1102 44-45 0.42640881250941476 1102 46-47 0.3904794451225584 1102 48-49 0.4237019209219497 1102 50-51 0.21403372859357006 1102 52-53 0.30339930804768045 1102 54-55 0.38724280745149287 1102 56-57 0.558819675299155 1102 58-59 0.3440386226952583 1102 60-61 0.4743975708374748 1102 62-63 0.49067182076152704 1102 64-65 0.5699435974360583 1102 66-67 0.34733137115168944 1102 68-69 0.32092458519224465 1102 70-71 0.4750056964552698 1102 72-73 0.5221767967024036 1102 74-75 0.6539941479188798 1102 76-77 0.6761113651407449 1102 78-79 0.6764324703418723 1102 80-81 0.6863413109454655 1102 82-83 0.539457508449452 1102 84-85 0.6244378702226321 1102 86-87 0.7878596366711292 1102 88-89 0.7335612127311428 1102 90-91 0.5810388938542346 1102 92-93 0.564444521936398 1102 94-95 0.5994505689207159 1102 96-97 0.5388194377583595 1102 98-99 0.5883871672452514 1102 100-101 0.9524182013496443 1103 1 0.2543584184627683 1103 2 0.2652389939454558 1103 3 0.24596260779336987 1103 4 0.25672982232418207 1103 5 0.15260181152908103 1103 6 0.16920080665283876 1103 7 0.18004126042726654 1103 8 0.23487744112446052 1103 9 0.26655364746029164 1103 10-11 0.2864500791133082 1103 12-13 0.1353642562358317 1103 14-15 0.23274617071076875 1103 16-17 0.4037799515916376 1103 18-19 0.4891910505604642 1103 20-21 0.27924066852425966 1103 22-23 0.21248932211467064 1103 24-25 0.5998767646735246 1103 26-27 0.5599686339217698 1103 28-29 0.521139641561355 1103 30-31 0.5950475661245918 1103 32-33 0.6344181227361148 1103 34-35 0.49818238909297463 1103 36-37 0.4816580259555181 1103 38-39 0.6359562165282284 1103 40-41 0.6754419086652277 1103 42-43 0.6608424039154599 1103 44-45 0.7763639787079484 1103 46-47 0.7253078434380882 1103 48-49 0.7417611576336967 1103 50-51 0.6520525447667325 1103 52-53 0.6333052271818573 1103 54-55 0.6163167234180165 1103 56-57 0.6321351081082582 1103 58-59 0.6669682043426874 1103 60-61 0.6405990713468626 1103 62-63 0.755317460499036 1103 64-65 0.7740339909588769 1103 66-67 0.6854624574330401 1103 68-69 0.7807284218437758 1103 70-71 0.5445341372536205 1103 72-73 0.5560371643643194 1103 74-75 0.6134766000432279 1103 76-77 0.5392644146229024 1103 78-79 0.5761142204686998 1103 80-81 0.6942389067483283 1103 82-83 0.633145552631575 1103 84-85 0.6638028553124045 1103 86-87 0.6484463119168531 1103 88-89 0.5998108054323446 1103 90-91 0.5332075382401094 1103 92-93 0.31853545165003183 1103 94-95 0.29276245462132877 1103 96-97 0.3551187312238433 1103 98-99 0.553905643143473 1103 100-101 0.874676719565695 1104 1 0.3139952714214225 1104 2 0.44980996315310406 1104 3 0.4350297498016893 1104 4 0.43541826383269466 1104 5 0.42135753485227667 1104 6 0.354439351268546 1104 7 0.32719724938256434 1104 8 0.4239727191643752 1104 9 0.40982039801077974 1104 10-11 0.44445111018527683 1104 12-13 0.5347381858433238 1104 14-15 0.5912878155326879 1104 16-17 0.6347771205126875 1104 18-19 0.7073556550831981 1104 20-21 0.6056808488744778 1104 22-23 0.6091289886764812 1104 24-25 0.5697678904643055 1104 26-27 0.6584229871425578 1104 28-29 0.669030924634292 1104 30-31 0.635705355086877 1104 32-33 0.6563452661424947 1104 34-35 0.6550907460186295 1104 36-37 0.646490782587577 1104 38-39 0.606679277588654 1104 40-41 0.6541073695019648 1104 42-43 0.6472905357528589 1104 44-45 0.5978629651117799 1104 46-47 0.5486352350007806 1104 48-49 0.6095976540861088 1104 50-51 0.6526694403663313 1104 52-53 0.732193067522978 1104 54-55 0.7007702552763888 1104 56-57 0.7449317597457465 1104 58-59 0.7106651216122728 1104 60-61 0.6647293883577916 1104 62-63 0.6140461837173845 1104 64-65 0.6575142957367603 1104 66-67 0.6159749351513994 1104 68-69 0.5934743936727926 1104 70-71 0.5261709203090845 1104 72-73 0.6187764080118754 1104 74-75 0.572521023516714 1104 76-77 0.5411030431224297 1104 78-79 0.6160437056317498 1104 80-81 0.7212864583893079 1104 82-83 0.6790091190920258 1104 84-85 0.8284290480354812 1104 86-87 0.8786580311856653 1104 88-89 0.8734903342848845 1104 90-91 0.8426574875602988 1104 92-93 0.6899122822396642 1104 94-95 0.7991822756691782 1104 96-97 0.7656097137131006 1104 98-99 0.8206672907615236 1104 100-101 0.8332022866552791 1105 1 0.3462180637401353 1105 2 0.32762177291051486 1105 3 0.34706038484051405 1105 4 0.3042330645109743 1105 5 0.31845546561058313 1105 6 0.2255732106201762 1105 7 0.3117436368131905 1105 8 0.25888624525985193 1105 9 0.33192270877871977 1105 10-11 0.44650697720818044 1105 12-13 0.48304038460816656 1105 14-15 0.34208951372499996 1105 16-17 0.5528725046082386 1105 18-19 0.53156670584751 1105 20-21 0.4965755909857634 1105 22-23 0.5510903413485977 1105 24-25 0.5622671239626129 1105 26-27 0.5860249475097277 1105 28-29 0.5075888533903097 1105 30-31 0.2904033847082559 1105 32-33 0.378202531014189 1105 34-35 0.3818469734537615 1105 36-37 0.499255976528147 1105 38-39 0.41280636356825084 1105 40-41 0.4233837136956353 1105 42-43 0.48223964942662434 1105 44-45 0.3485563362800548 1105 46-47 0.4163354796556007 1105 48-49 0.5782766744656698 1105 50-51 0.48807415325669723 1105 52-53 0.45399284476820156 1105 54-55 0.5480956336779386 1105 56-57 0.6264055704167006 1105 58-59 0.7117980580333025 1105 60-61 0.565097856668423 1105 62-63 0.5609264436396657 1105 64-65 0.6547376824853544 1105 66-67 0.6705901356619819 1105 68-69 0.6927089869888974 1105 70-71 0.7101454036243808 1105 72-73 0.7527957082114725 1105 74-75 0.6347734747578215 1105 76-77 0.43786543385683174 1105 78-79 0.4947256512320273 1105 80-81 0.6017149328319533 1105 82-83 0.7736857648806463 1105 84-85 0.777197013896437 1105 86-87 0.6568586450127292 1105 88-89 0.6446692451642058 1105 90-91 0.5247841543103959 1105 92-93 0.5618122522053106 1105 94-95 0.6466855919621928 1105 96-97 0.6633454833746448 1105 98-99 0.7904667332488664 1105 100-101 0.9143341437167898 1106 1 0.6409035461807981 1106 2 0.606495441127084 1106 3 0.6879292918227442 1106 4 0.5129173804126523 1106 5 0.5306453408567364 1106 6 0.5873648535934279 1106 7 0.5691423828752065 1106 8 0.5643407292126241 1106 9 0.6800861412556785 1106 10-11 0.770390902922685 1106 12-13 0.7694967847566332 1106 14-15 1.035131602378307 1106 16-17 1.0404506014675476 1106 18-19 1.0484614083343615 1106 20-21 1.1032903667195626 1106 22-23 1.122129914080844 1106 24-25 0.995467454177259 1106 26-27 1.0514449746802157 1106 28-29 1.03652994500019 1106 30-31 1.1212015222684357 1106 32-33 1.1575582193544705 1106 34-35 1.1151833389351822 1106 36-37 1.0984965834209675 1106 38-39 1.1611440912307671 1106 40-41 1.2513900656649426 1106 42-43 1.2264972333013233 1106 44-45 1.2229401550313383 1106 46-47 1.0532150244494218 1106 48-49 1.2018363279414288 1106 50-51 1.132794238754137 1106 52-53 1.250547212249927 1106 54-55 1.1656477485326633 1106 56-57 1.3909221698275758 1106 58-59 1.4058320705068539 1106 60-61 1.4051309421274638 1106 62-63 1.4259203549644255 1106 64-65 1.2852878848012423 1106 66-67 1.475581340908846 1106 68-69 1.4090219741514858 1106 70-71 1.4251487770795848 1106 72-73 1.5213625108524624 1106 74-75 1.5177516048945705 1106 76-77 1.4484904464273036 1106 78-79 1.5708478050506223 1106 80-81 1.6799649232326885 1106 82-83 1.6576130792629016 1106 84-85 1.745796911138612 1106 86-87 1.7608917981237155 1106 88-89 1.7500734929751154 1106 90-91 1.7501531316872203 1106 92-93 1.803980396548969 1106 94-95 1.8987233783173387 1106 96-97 1.7943223957763443 1106 98-99 1.900356807976845 1106 100-101 2.076507974480876 1107 1 0.2123341164015642 1107 2 0.3337381925738079 1107 3 0.39875343115614825 1107 4 0.41057435622406757 1107 5 0.3461490924564359 1107 6 0.3472093049196374 1107 7 0.36576547676033755 1107 8 0.37151578023512855 1107 9 0.43310178566894564 1107 10-11 0.41233326521789593 1107 12-13 0.41071974842711967 1107 14-15 0.24698598583619713 1107 16-17 0.41049246417130547 1107 18-19 0.45148064391094067 1107 20-21 0.5812662751439674 1107 22-23 0.6775287384179194 1107 24-25 0.6517826314594615 1107 26-27 0.6323030481749967 1107 28-29 0.48790629331877966 1107 30-31 0.6928993310058758 1107 32-33 0.6323496520514098 1107 34-35 0.7282466851904772 1107 36-37 0.6931309656849081 1107 38-39 0.8191979512182996 1107 40-41 0.8513216470080565 1107 42-43 0.87125119663974 1107 44-45 0.8537156317699299 1107 46-47 0.726028332461162 1107 48-49 0.5945302694157206 1107 50-51 0.49118324289189985 1107 52-53 0.5571812568512442 1107 54-55 0.7193074237795685 1107 56-57 0.6389174145676861 1107 58-59 0.6962872248056229 1107 60-61 0.7919183527214884 1107 62-63 0.7287780814688816 1107 64-65 0.7798185897139476 1107 66-67 0.7655435264255459 1107 68-69 0.7858590640592951 1107 70-71 0.6893952017733511 1107 72-73 0.7246846167479433 1107 74-75 0.885773554830962 1107 76-77 0.911167299560848 1107 78-79 0.914995747920333 1107 80-81 0.8259226258582473 1107 82-83 0.7566251828824235 1107 84-85 0.6680377317520758 1107 86-87 0.8130154348808105 1107 88-89 0.6793117186232998 1107 90-91 0.6927425563175653 1107 92-93 0.8052746144962022 1107 94-95 0.7586174063526663 1107 96-97 0.6869841566962833 1107 98-99 0.5036943978492019 1107 100-101 0.4383811066095653 1108 1 0.01706668223758001 1108 2 0.13837297812395732 1108 3 0.13253836982087108 1108 4 -0.0015707684224182117 1108 5 -0.01728782970476317 1108 6 0.011789585583620976 1108 7 0.023993697980408513 1108 8 -0.14252514355886348 1108 9 -0.11404172895703368 1108 10-11 -0.022055959679200043 1108 12-13 -0.08197529173395424 1108 14-15 0.23155337007327859 1108 16-17 0.0944181983162693 1108 18-19 0.1531783007619083 1108 20-21 0.13390845703074916 1108 22-23 0.14876801917785087 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2316 56-57 -2.0497959341579772 2316 58-59 -2.0118535325796074 2316 60-61 -2.0376594919396425 2316 62-63 -1.9115410689293242 2316 64-65 -2.0521564046169942 2316 66-67 -1.8656700971631999 2316 68-69 -1.3584849408332715 2316 70-71 -1.831389343362602 2316 72-73 -1.6651625421985088 2316 74-75 -2.0186931420706458 2316 76-77 -2.333977501747505 2316 78-79 -2.565866480394682 2316 80-81 -2.3496852510305786 2316 82-83 -2.4378639840762872 2316 84-85 -2.0601619322278033 2316 86-87 -1.9231749284194706 2316 88-89 -2.228516038425223 2316 90-91 -2.361738570895941 2316 92-93 -2.705509466808298 2316 94-95 -2.9111571887396543 2316 96-97 -2.8293166843847537 2316 98-99 -2.949052429200073 2316 100-101 -2.673119680186012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5727.0 3 1715.0 4 1819.0 5 1890.0 6 2176.0 7 2530.0 8 2995.0 9 3813.0 10 4810.0 11 5822.0 12 7261.0 13 8909.0 14 9909.0 15 11819.0 16 14929.0 17 19369.0 18 17231.0 19 12052.0 20 11635.0 21 12504.0 22 13503.0 23 15347.0 24 17424.0 25 20436.0 26 23608.0 27 28096.0 28 33874.0 29 40611.0 30 50892.0 31 62718.0 32 79979.0 33 104828.0 34 142495.0 35 204441.0 36 309231.0 37 457553.0 38 417273.0 39 75202.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.900436060046275 18.65715322052116 14.70547544911879 22.736935270313772 2 15.212404811850504 20.147040101563597 40.70870064155937 23.93185444502653 3 19.330707142467528 24.755064020608636 29.85201179435533 26.062217042568502 4 12.70059188611506 16.81561809035415 37.890162727636984 32.5936272958938 5 13.843475020462304 37.22107495303097 34.10166604189172 14.833783984615009 6 31.28098135994584 36.04954888212023 18.041299311766863 14.628170446167074 7 28.123476343535035 32.71049473499746 21.108412247369717 18.057616674097783 8 26.05462766587454 34.38141419052389 20.158862323607682 19.405095819993885 9 26.25095558413866 17.069095333022457 20.655747015005833 36.02420206783305 10-11 24.516669273060206 26.677602428493312 27.67519678271218 21.1305315157343 12-13 25.983879824808092 24.080481999536914 27.975104260832612 21.96053391482238 14-15 22.83075136447192 25.268857747148626 26.656462699702622 25.243928188676833 16-17 21.98103826175415 28.590695345809603 27.00214346033866 22.42612293209758 18-19 21.939304826472746 27.320789782417293 29.188261933117328 21.551643457992633 20-21 22.936149453960862 26.255069571484007 29.120860358112978 21.687920616442153 22-23 22.561978339734434 26.25001108735952 29.12270159037085 22.065308982535193 24-25 22.449401765381346 26.34906268853154 29.206372152640235 21.99516339344688 26-27 22.217379235974825 26.469560575116198 29.224401910905414 22.08865827800357 28-29 21.91705615584327 26.73933090862971 29.241233915902264 22.102379019624756 30-31 22.21209572400669 26.338201590079453 29.17958959083676 22.270113095077104 32-33 22.0404517557589 26.478709998944744 29.240553024814464 22.240285220481887 34-35 22.523515106640982 26.42495006572924 28.91558946824712 22.135945359382657 36-37 22.432278683625483 26.245995071266226 29.06540725901924 22.256318986089052 38-39 22.231590064785912 26.45711765993017 29.16634059036917 22.144951684914744 40-41 22.39250874313293 26.43138938265599 29.005206302017843 22.170895572193235 42-43 22.202824529690332 26.465471158841158 28.741918726427425 22.589785585041085 44-45 22.380350978001857 26.502361733772183 28.600710149525117 22.516577138700843 46-47 22.56840846198111 26.69016182532512 28.35782451293246 22.38360519976131 48-49 22.696331114972793 26.81196961464548 28.04553502568949 22.44616424469224 50-51 22.33292513312683 26.936758520948427 28.214044672987303 22.516271672937435 52-53 22.640271415476935 27.08034399096464 27.776572127155625 22.5028124664028 54-55 22.459618136901078 26.76151911709676 27.949232709131767 22.829630036870398 56-57 22.570817711344667 27.089858506461027 27.77435877426655 22.564965007927754 58-59 22.860163145620312 26.812408254699253 27.76464161328506 22.562786986395366 60-61 22.66023055943408 26.702514125634007 28.340413701610036 22.29684161332188 62-63 22.48361468633163 26.85067409205929 28.23349013777166 22.432221083837423 64-65 22.37866006018894 27.030290428469332 27.99563656806014 22.59541294328159 66-67 22.415201418664267 27.058697278454545 27.687123156217584 22.8389781466636 68-69 22.258142325192857 26.934143094930462 28.062544727696455 22.74516985218023 70-71 22.449579380268826 26.82300503458158 27.882073542791723 22.845342042357863 72-73 22.484046491846108 26.804619752097565 27.90758545457608 22.803748301480248 74-75 22.360742000048763 27.235542236772403 27.648966328943896 22.754749434234935 76-77 22.470138669364655 26.98133769362038 27.603348069105465 22.945175567909505 78-79 22.4405194256944 27.03358511750667 27.68027312241642 22.845622334382508 80-81 22.502032165742264 27.10921292065055 27.58777770068565 22.800977212921538 82-83 22.441185626119108 27.007364489926594 27.83604697877654 22.715402905177758 84-85 22.42097204019174 27.024614413798776 27.633339622946906 22.92107392306258 86-87 22.44039283126402 26.992094062387196 27.72667930221276 22.840833804136025 88-89 22.478885971493707 26.883041333839287 27.500854408180675 23.137218286486334 90-91 22.43734889322882 26.762306277349996 27.75083058835633 23.049514241064855 92-93 22.545510586706552 26.84514259928398 27.74004277479591 22.869304039213556 94-95 22.38481768218974 26.769218009420868 27.759737487722735 23.086226820666656 96-97 22.178403151956832 26.863091033490093 27.903667044064633 23.054838770488438 98-99 22.20310116145469 26.9087086805943 27.493484801123095 23.39470535682791 100-101 22.184305262606806 26.705531118921765 27.74774590909454 23.362417709376896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 331.0 1 257.0 2 215.0 3 263.0 4 259.0 5 261.5 6 397.0 7 812.5 8 1437.5 9 1867.0 10 1841.5 11 1763.0 12 1882.5 13 2037.5 14 2234.5 15 2378.5 16 2502.5 17 2653.5 18 2797.5 19 3006.0 20 3375.0 21 3906.0 22 4556.5 23 5562.5 24 6925.0 25 8699.0 26 11201.5 27 14236.0 28 16950.5 29 19924.0 30 24120.0 31 29050.5 32 33626.5 33 37596.5 34 43046.0 35 47992.0 36 52687.5 37 58471.5 38 61617.0 39 63498.5 40 66062.5 41 69893.0 42 73702.5 43 78197.5 44 83011.0 45 85944.0 46 87294.5 47 90872.5 48 99823.5 49 99516.0 50 90334.5 51 84195.5 52 79927.0 53 77389.0 54 74329.0 55 68588.5 56 62131.0 57 55684.0 58 50206.0 59 44996.5 60 38384.0 61 30819.5 62 23666.5 63 18235.0 64 13628.0 65 10078.5 66 7300.5 67 5335.0 68 3723.0 69 2304.0 70 1539.0 71 1050.0 72 705.0 73 477.5 74 343.0 75 236.5 76 156.5 77 78.5 78 39.0 79 35.0 80 28.0 81 16.0 82 17.5 83 18.0 84 13.0 85 11.5 86 10.5 87 7.0 88 4.0 89 4.5 90 5.0 91 5.5 92 3.0 93 2.5 94 3.5 95 1.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05606128894874669 2 0.09342070917308934 3 0.06567812665774118 4 0.12479730409460131 5 0.10255005741296429 6 0.10108758900560569 7 0.10742495210415966 8 0.09661154933459903 9 0.11531341805900307 10-11 0.10576305618670669 12-13 0.08938784174673682 14-15 0.09337639194862393 16-17 0.10607327675796459 18-19 0.08482316762679934 20-21 0.07019848355321325 22-23 0.07263593089881093 24-25 0.06051517000752063 26-27 0.052870448787237 28-29 0.065390064698716 30-31 0.04874894691195364 32-33 0.0479069196471108 34-35 0.044051321118619924 36-37 0.049325070830004 38-39 0.04908132609544423 40-41 0.06219922453720631 42-43 0.0586095293555079 44-45 0.04513709311802253 46-47 0.036207172388241934 48-49 0.04274396299689026 50-51 0.027543155005253804 52-53 0.03884404724393397 54-55 0.04391836944522369 56-57 0.048350091891764924 58-59 0.05470961360255161 60-61 0.05149661482880921 62-63 0.05825499155978461 64-65 0.0396639158965441 66-67 0.06115776976226912 68-69 0.050743222012897195 70-71 0.024884121537329062 72-73 0.019964909621668286 74-75 0.029426637045033833 76-77 0.04210136324214178 78-79 0.059961204701702984 80-81 0.03813497165248737 82-83 0.05818851572308648 84-85 0.06286398290418749 86-87 0.06403838935252093 88-89 0.08750435970695679 90-91 0.06341794821000514 92-93 0.07208196559299326 94-95 0.07903976983406302 96-97 0.08803616640054174 98-99 0.11923549242419207 100-101 0.1133191429580595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2256459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.76675964200957 #Duplication Level Percentage of deduplicated Percentage of total 1 77.70102957741094 44.88536699534975 2 12.824673339380425 14.816796445665542 3 4.133752784176894 7.163805105091036 4 1.7345790345535703 4.008040406765004 5 0.9655714952116345 2.7888968240533143 6 0.5675044626645364 1.966973633430604 7 0.3879807926359407 1.568867523574172 8 0.2779173849112057 1.2843509419601182 9 0.20024141980382695 1.0410568171364165 >10 1.0871627553642236 11.534288520000088 >50 0.07900275560388569 3.1222304816213775 >100 0.03828083255673971 3.737545592467294 >500 0.0013052405782227009 0.47741201170724845 >1k 8.445674329673447E-4 0.9921246088699862 >5k 1.5355771508497175E-4 0.6122440923081626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7904 0.3502833421746196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5900 0.2614716243459332 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4192 0.18577780495900878 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3682 0.16317602048164848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2949 0.13069149494850116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 4 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 5 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 6 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 7 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 8 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 9 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 10-11 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 12-13 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 14-15 0.0 4.43172244654124E-5 0.0 4.43172244654124E-5 0.0 16-17 0.0 4.43172244654124E-5 0.0 1.329516733962372E-4 0.0 18-19 0.0 4.43172244654124E-5 0.0 3.9885502018871164E-4 0.0 20-21 0.0 4.43172244654124E-5 0.0 5.539653058176551E-4 0.0 22-23 0.0 4.43172244654124E-5 0.0 6.64758366981186E-4 0.0 24-25 0.0 4.43172244654124E-5 0.0 9.085031015409542E-4 0.0 26-27 4.43172244654124E-5 4.43172244654124E-5 0.0 9.97137550471779E-4 0.0 28-29 4.43172244654124E-5 4.43172244654124E-5 0.0 0.0017062131419183775 0.0 30-31 4.43172244654124E-5 4.43172244654124E-5 0.0 0.0033016332226732236 0.0 32-33 4.43172244654124E-5 4.43172244654124E-5 0.0 0.005052163589057013 0.0 34-35 4.43172244654124E-5 4.43172244654124E-5 0.0 0.006293045874088561 0.0 36-37 4.43172244654124E-5 4.43172244654124E-5 0.0 0.007954941791541527 0.0 38-39 4.43172244654124E-5 4.43172244654124E-5 0.0 0.01014864440257944 0.0 40-41 4.43172244654124E-5 4.43172244654124E-5 0.0 0.014447415175724444 0.0 42-43 4.43172244654124E-5 4.43172244654124E-5 0.0 0.01976548211157393 0.0 44-45 4.43172244654124E-5 4.43172244654124E-5 0.0 0.030224347085411257 0.0 46-47 4.43172244654124E-5 4.43172244654124E-5 0.0 0.052294324869186636 0.0 48-49 4.43172244654124E-5 4.43172244654124E-5 0.0 0.06680821588160919 0.0 50-51 4.43172244654124E-5 4.43172244654124E-5 0.0 0.08409193342312003 0.0 52-53 4.43172244654124E-5 4.43172244654124E-5 0.0 0.10407900165702103 0.0 54-55 4.43172244654124E-5 4.43172244654124E-5 0.0 0.13213180474362707 0.0 56-57 4.43172244654124E-5 4.43172244654124E-5 0.0 0.15987438725897524 0.0 58-59 4.43172244654124E-5 4.43172244654124E-5 0.0 0.19956046176775205 0.0 60-61 4.43172244654124E-5 4.43172244654124E-5 0.0 0.2434345139885103 0.0 62-63 4.43172244654124E-5 4.43172244654124E-5 0.0 0.29597258359225675 0.0 64-65 4.43172244654124E-5 4.43172244654124E-5 0.0 0.339093242997103 0.0 66-67 4.43172244654124E-5 4.43172244654124E-5 0.0 0.4075411961839324 0.0 68-69 4.43172244654124E-5 4.43172244654124E-5 0.0 0.49209846046393924 0.0 70-71 4.43172244654124E-5 4.43172244654124E-5 0.0 0.5489796180652962 0.0 72-73 4.43172244654124E-5 4.43172244654124E-5 0.0 0.6555847015168457 0.0 74-75 4.43172244654124E-5 4.43172244654124E-5 0.0 0.7411169447350916 0.0 76-77 4.43172244654124E-5 4.43172244654124E-5 0.0 0.8121131383286821 0.0 78-79 4.43172244654124E-5 4.43172244654124E-5 0.0 0.9018998350956078 0.0 80-81 4.43172244654124E-5 4.43172244654124E-5 0.0 0.9952762270442317 0.0 82-83 4.43172244654124E-5 4.43172244654124E-5 0.0 1.115929870651317 0.0 84-85 4.43172244654124E-5 4.43172244654124E-5 0.0 1.2980293459796965 0.0 86-87 4.43172244654124E-5 4.43172244654124E-5 0.0 1.542660425028773 0.0 88-89 4.43172244654124E-5 4.43172244654124E-5 0.0 1.832805293603828 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14025 0.0 27.417982 1 GGTATCA 10100 0.0 25.178057 1 ACCGCGC 140 1.7735283E-5 23.761984 8 GTACATG 10440 0.0 23.219854 1 TATCAAC 16375 0.0 23.193428 2 ATCAACG 16260 0.0 23.149748 3 TCAACGC 16300 0.0 23.092937 4 CAACGCA 16455 0.0 22.904297 5 AACGCAG 16715 0.0 22.633322 6 GAGTACT 6940 0.0 22.284754 12-13 TACATGG 10685 0.0 22.109621 2 ACATGGG 10460 0.0 21.809774 3 ACGCAGA 18255 0.0 20.437124 7 CGCAGAG 18240 0.0 20.27064 8 GTACTTT 7610 0.0 20.229778 14-15 AGAGTAC 12875 0.0 20.075796 10-11 AGTACTT 7595 0.0 19.67475 12-13 GCAGAGT 18910 0.0 18.798481 9 CATGGGA 6355 0.0 18.023666 4 CATGGGG 4775 0.0 17.517855 4 >>END_MODULE