FastQCFastQC Report
Thu 26 May 2016
SRR937035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256459
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.3498844871544309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64880.2875301523315956No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53210.2358119513804594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.17274854096617753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA156700.024.7684671
GTACATG98150.023.0389541
GGTATCA115900.022.8306271
GAGTACT83100.022.40497612-13
TACATGG100700.021.8865262
GTACTTT86850.021.35649314-15
ACATGGG99050.020.8120083
TCAACGC184350.020.7921544
TATCAAC185500.020.6897772
CAACGCA185550.020.6576845
ATCAACG185400.020.6492393
AGAGTAC139450.020.50394610-11
AACGCAG189150.020.3394016
AGTACTT88200.020.32861912-13
GTACCGT1759.9481724E-518.9985076
GTGTAGC10550.018.9122371
CATGGGG43500.018.562174
ACGCAGA208350.018.3054967
CGCAGAG209050.018.221488
TACTTTT98700.018.0224314-15
Produced by FastQC (version 0.11.5)