##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937035_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256459 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.179796752345155 33.0 31.0 34.0 31.0 34.0 2 32.16775886466362 33.0 31.0 34.0 30.0 34.0 3 32.35561027255536 34.0 31.0 34.0 31.0 34.0 4 36.07285086943747 37.0 35.0 37.0 35.0 37.0 5 35.999111882821715 37.0 35.0 37.0 35.0 37.0 6 36.046537074238884 37.0 35.0 37.0 35.0 37.0 7 35.98805739435106 37.0 35.0 37.0 35.0 37.0 8 35.96695175937165 37.0 35.0 37.0 35.0 37.0 9 37.66348601946678 39.0 37.0 39.0 35.0 39.0 10-11 37.591672173081804 39.0 37.0 39.0 35.0 39.0 12-13 37.50046510927076 39.0 37.0 39.0 35.0 39.0 14-15 38.73403771129898 40.0 38.0 41.0 35.0 41.0 16-17 38.655507811132395 40.0 38.0 41.0 34.0 41.0 18-19 38.70725814207127 40.0 38.0 41.0 34.5 41.0 20-21 38.779639913687774 40.0 38.0 41.0 35.0 41.0 22-23 38.715022741383734 40.0 38.0 41.0 34.0 41.0 24-25 38.61537922913733 40.0 38.0 41.0 34.0 41.0 26-27 38.46902979402684 40.0 38.0 41.0 34.0 41.0 28-29 38.30952833621174 40.0 38.0 41.0 34.0 41.0 30-31 38.19907341547088 40.0 38.0 41.0 34.0 41.0 32-33 38.20430550699126 40.0 38.0 41.0 34.0 41.0 34-35 38.12786582871659 40.0 38.0 41.0 34.0 41.0 36-37 37.98779060465978 40.0 37.5 41.0 33.0 41.0 38-39 37.84534374433571 40.0 37.0 41.0 33.0 41.0 40-41 37.68755647676293 40.0 37.0 41.0 33.0 41.0 42-43 37.48264803393281 40.0 37.0 41.0 33.0 41.0 44-45 37.175756572576766 40.0 37.0 41.0 31.5 41.0 46-47 36.93035725444159 40.0 36.0 41.0 31.0 41.0 48-49 36.6296365234201 39.0 36.0 41.0 30.5 41.0 50-51 36.386033825564745 39.0 36.0 41.0 30.0 41.0 52-53 36.143761087615594 39.0 35.0 40.5 30.0 41.0 54-55 35.91431397601286 39.0 35.0 40.0 29.0 41.0 56-57 35.648263496035156 38.5 35.0 40.0 28.5 41.0 58-59 35.32894858714472 38.0 34.5 40.0 28.0 41.0 60-61 35.00109419227205 38.0 34.0 40.0 27.5 41.0 62-63 34.6428867087769 37.5 34.0 40.0 26.5 41.0 64-65 34.248748149201916 37.0 34.0 39.5 26.0 41.0 66-67 33.87024647910731 36.0 33.0 39.0 26.0 40.5 68-69 33.436401237514175 36.0 33.0 39.0 25.5 40.0 70-71 32.95953106172104 35.0 32.0 38.0 25.0 40.0 72-73 32.61854392213641 35.0 32.0 37.5 24.0 39.5 74-75 32.17632294670544 35.0 31.5 37.0 23.0 39.0 76-77 30.756920910151702 33.5 29.5 35.5 21.5 38.0 78-79 31.495751529276625 34.0 31.0 36.0 22.0 38.5 80-81 31.32594099870638 34.0 31.0 36.0 21.5 37.0 82-83 31.00356399119151 34.0 31.0 35.0 20.5 37.0 84-85 30.636525192791005 34.0 31.0 35.0 19.5 36.5 86-87 30.293435200905492 34.0 31.0 35.0 18.0 36.0 88-89 29.952712413564793 34.0 30.0 35.0 16.0 36.0 90-91 29.55108357829679 34.0 30.0 35.0 8.5 35.0 92-93 29.115238078777413 34.0 29.0 35.0 4.5 35.0 94-95 28.665435312584897 34.0 29.0 35.0 2.0 35.0 96-97 28.073788621907156 33.5 29.0 35.0 2.0 35.0 98-99 27.38369852942154 33.0 27.5 35.0 2.0 35.0 100-101 24.403010867912954 30.5 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2793316127098393 1101 2 0.12124140066634936 1101 3 0.16871843423759714 1101 4 0.18393891868711165 1101 5 0.24977820821168706 1101 6 0.1623408334918537 1101 7 0.2126139659594557 1101 8 0.24236868830355718 1101 9 0.3243847508973374 1101 10-11 0.30859402470263575 1101 12-13 0.30574785173495655 1101 14-15 0.3756698588086209 1101 16-17 0.3811492034682544 1101 18-19 0.35173962503822054 1101 20-21 0.34426586426243233 1101 22-23 0.2970977481525736 1101 24-25 0.39526598853949935 1101 26-27 0.40891081929904516 1101 28-29 0.312714226591261 1101 30-31 0.34236108112386887 1101 32-33 0.4312333306646181 1101 34-35 0.3308234969909165 1101 36-37 0.41914319426162194 1101 38-39 0.4095231047406003 1101 40-41 0.36112434269480076 1101 42-43 0.4815260737625522 1101 44-45 0.5950957224332214 1101 46-47 0.5136746077180092 1101 48-49 0.4257838315467808 1101 50-51 0.5161836620289364 1101 52-53 0.5107549287707016 1101 54-55 0.5188356413462856 1101 56-57 0.6802831768642577 1101 58-59 0.6592182646089029 1101 60-61 0.623111809865776 1101 62-63 0.7222786415781925 1101 64-65 0.6395242127928427 1101 66-67 0.6245310645547377 1101 68-69 0.4884450102847637 1101 70-71 0.44137776860413425 1101 72-73 0.5235454541443403 1101 74-75 0.5746635398331037 1101 76-77 0.4242921467403278 1101 78-79 0.5949050695122828 1101 80-81 0.6619544860052713 1101 82-83 0.8164079520055729 1101 84-85 0.8595271900023569 1101 86-87 0.6226582839428829 1101 88-89 0.7001306952580144 1101 90-91 0.7925495818383226 1101 92-93 0.8023693505732865 1101 94-95 0.7394433411193084 1101 96-97 0.7358047981410429 1101 98-99 0.8185997929932576 1101 100-101 0.7317020115809072 1102 1 0.23279761521354914 1102 2 0.11549707833285083 1102 3 0.14626750770474928 1102 4 0.17352868814188582 1102 5 0.15695383440712618 1102 6 0.19761771879412038 1102 7 0.13976904805526402 1102 8 0.21273824032264343 1102 9 0.32690608747940075 1102 10-11 0.3211063484760359 1102 12-13 0.2672814764958744 1102 14-15 0.3101660649668858 1102 16-17 0.39967977650022135 1102 18-19 0.3381864125293532 1102 20-21 0.2529202593162623 1102 22-23 0.2587248086068996 1102 24-25 0.2441982695725713 1102 26-27 0.25656060519447266 1102 28-29 0.2785262490870153 1102 30-31 0.3334693275079985 1102 32-33 0.44413895742419385 1102 34-35 0.3120644976141591 1102 36-37 0.3741866901299673 1102 38-39 0.4679878244973281 1102 40-41 0.3007202236666231 1102 42-43 0.39882062605026647 1102 44-45 0.5695102088777375 1102 46-47 0.5779963938659591 1102 48-49 0.48283165316033205 1102 50-51 0.6279915159327842 1102 52-53 0.7088239076503768 1102 54-55 0.5592943879345782 1102 56-57 0.5095836365040682 1102 58-59 0.6760910886401845 1102 60-61 0.6177296452873193 1102 62-63 0.6633559777895286 1102 64-65 0.5396658018678622 1102 66-67 0.5893661677655047 1102 68-69 0.4815603191648705 1102 70-71 0.4793764616797205 1102 72-73 0.6699064654862497 1102 74-75 0.3861243300858348 1102 76-77 0.42518788694578546 1102 78-79 0.5194542779700875 1102 80-81 0.6582581095304505 1102 82-83 0.6128663950328033 1102 84-85 0.597036693075573 1102 86-87 0.5130799584716925 1102 88-89 0.6388930425372443 1102 90-91 0.6276980413330691 1102 92-93 0.47000137948949217 1102 94-95 0.648909443019118 1102 96-97 0.6540682945198029 1102 98-99 0.7512316506526666 1102 100-101 0.932950406468187 1103 1 -0.08234053641296413 1103 2 -0.1926247277228086 1103 3 -0.06631994295538135 1103 4 0.05894141071263448 1103 5 0.06353801017872485 1103 6 0.11259402328451529 1103 7 0.12267742954265515 1103 8 0.1373112055816037 1103 9 0.052644154140295996 1103 10-11 0.14542433437834035 1103 12-13 0.15962120699811777 1103 14-15 0.1708806010999595 1103 16-17 0.2158705119477915 1103 18-19 0.30829938381015154 1103 20-21 0.20241603372016215 1103 22-23 0.2612584339579982 1103 24-25 0.29075052136777657 1103 26-27 0.261755881765815 1103 28-29 0.21447932826590232 1103 30-31 0.26419438123019034 1103 32-33 0.32555425371086955 1103 34-35 0.31018288029767405 1103 36-37 0.41249471938123605 1103 38-39 0.38268398990807384 1103 40-41 0.3619923982503579 1103 42-43 0.2991621383558467 1103 44-45 0.4256879107638909 1103 46-47 0.38805450870153635 1103 48-49 0.37871395515124817 1103 50-51 0.07258650626072694 1103 52-53 0.22039366813806538 1103 54-55 0.17105404246271405 1103 56-57 0.34956763327042495 1103 58-59 0.4522741524345193 1103 60-61 0.4562364775957022 1103 62-63 0.3853334495260867 1103 64-65 0.23807941946481748 1103 66-67 0.4017833572182141 1103 68-69 0.4279932891258085 1103 70-71 0.4371122515919055 1103 72-73 0.3956747238147358 1103 74-75 0.3408430155109343 1103 76-77 0.4435393888987562 1103 78-79 0.40803646571111685 1103 80-81 0.447153432709154 1103 82-83 0.4186931394058888 1103 84-85 0.48656018442021676 1103 86-87 0.29666031079030475 1103 88-89 0.5236659657258507 1103 90-91 0.7285205580477871 1103 92-93 0.7580752583351149 1103 94-95 0.6992448097530151 1103 96-97 1.020417371240459 1103 98-99 1.082311332732754 1103 100-101 1.1960838231173199 1104 1 0.26038726058818895 1104 2 0.09302904180639615 1104 3 0.1749487998358461 1104 4 0.11721183118028478 1104 5 0.18556660058101926 1104 6 0.13139815456269588 1104 7 0.25499297251563746 1104 8 0.22600384128116247 1104 9 0.2572422108317767 1104 10-11 0.2183711031036566 1104 12-13 0.2811956014617891 1104 14-15 0.3045385473444071 1104 16-17 0.3538424458193816 1104 18-19 0.3335358313303374 1104 20-21 0.2307569690247675 1104 22-23 0.3128223429440524 1104 24-25 0.33678037002739103 1104 26-27 0.1961635396596293 1104 28-29 0.2836119171303224 1104 30-31 0.4135016406758609 1104 32-33 0.4292435690091807 1104 34-35 0.3764777494039606 1104 36-37 0.43411158002924566 1104 38-39 0.3042001899709845 1104 40-41 0.45133303646642986 1104 42-43 0.28463713049174544 1104 44-45 0.4629099582281526 1104 46-47 0.45237330217257465 1104 48-49 0.5198909500133624 1104 50-51 0.49419882320176356 1104 52-53 0.5406625332598693 1104 54-55 0.5256920313481004 1104 56-57 0.5101397034532198 1104 58-59 0.4813334303346295 1104 60-61 0.3287323707836407 1104 62-63 0.47681233876449625 1104 64-65 0.420345243798117 1104 66-67 0.42571567346022476 1104 68-69 0.412518209610937 1104 70-71 0.4580285949563532 1104 72-73 0.3445159911594615 1104 74-75 0.4013835418819056 1104 76-77 0.4088677591060197 1104 78-79 0.4340303142309132 1104 80-81 0.580799670170272 1104 82-83 0.6540527912880343 1104 84-85 0.8315709320347686 1104 86-87 0.6743038744546297 1104 88-89 0.7524414366985503 1104 90-91 0.9594555131349907 1104 92-93 1.012444644235643 1104 94-95 0.8140184981945247 1104 96-97 1.037064814531501 1104 98-99 1.1832611422465824 1104 100-101 1.1982225110345581 1105 1 0.07952805912651684 1105 2 -0.016378906033949647 1105 3 0.05399952660459206 1105 4 0.036636078913630854 1105 5 0.044531854590296405 1105 6 0.08850612805737512 1105 7 0.06838422626211127 1105 8 0.13573888198396133 1105 9 0.1434346997409648 1105 10-11 0.16200350766637683 1105 12-13 0.20780384714279165 1105 14-15 0.304617152874485 1105 16-17 0.2907358926992458 1105 18-19 0.22241029399700807 1105 20-21 0.17950107947609695 1105 22-23 0.24675928331960506 1105 24-25 0.20449547542919078 1105 26-27 0.3103507819429936 1105 28-29 0.23667972280806993 1105 30-31 0.4282747874624988 1105 32-33 0.28158718702228924 1105 34-35 0.2617742916992114 1105 36-37 0.20230895577775954 1105 38-39 0.2709175917492672 1105 40-41 0.09373340689010234 1105 42-43 0.09640856281745869 1105 44-45 0.3181029289384014 1105 46-47 0.19054962258905306 1105 48-49 0.28664370472656486 1105 50-51 0.23950204501171157 1105 52-53 0.3000564807122714 1105 54-55 0.18089649958364618 1105 56-57 0.22469330225684558 1105 58-59 0.24210199688261724 1105 60-61 0.25589828554865335 1105 62-63 0.11538450255182653 1105 64-65 0.11570266822730702 1105 66-67 0.1616186906611432 1105 68-69 0.043940007667465864 1105 70-71 -0.055987826951202635 1105 72-73 0.06859689306601524 1105 74-75 0.160456418985099 1105 76-77 0.16018176199682443 1105 78-79 0.07276320872899689 1105 80-81 0.2104076749507371 1105 82-83 0.20602395205315815 1105 84-85 0.2853497071337934 1105 86-87 0.3596572786606913 1105 88-89 0.227697510833341 1105 90-91 0.3626408751427874 1105 92-93 0.4602306275790937 1105 94-95 0.4073604142610243 1105 96-97 0.6772337264745367 1105 98-99 0.8968523121471534 1105 100-101 0.7747663080023415 1106 1 0.3777268717434197 1106 2 0.24022081567885323 1106 3 0.27735380156770617 1106 4 0.18951713764832334 1106 5 0.17776276531349566 1106 6 0.2742344926904039 1106 7 0.2766813718984693 1106 8 0.28847869442351026 1106 9 0.4610546493859786 1106 10-11 0.4231697358143407 1106 12-13 0.4938072535097504 1106 14-15 0.6533457990858764 1106 16-17 0.624587510037891 1106 18-19 0.5442717464660305 1106 20-21 0.4215645461447153 1106 22-23 0.5024537031310246 1106 24-25 0.5504298219570884 1106 26-27 0.6158928020301389 1106 28-29 0.6160291030613863 1106 30-31 0.6435345983661591 1106 32-33 0.7378243765775565 1106 34-35 0.6048709133072379 1106 36-37 0.6969850217987457 1106 38-39 0.8505578753754861 1106 40-41 0.8714014534126946 1106 42-43 0.8133592152930618 1106 44-45 1.0101213247827445 1106 46-47 0.7662791208492976 1106 48-49 0.6917568206106992 1106 50-51 0.7474596052996461 1106 52-53 0.8074538554510369 1106 54-55 0.8977865852822049 1106 56-57 0.7965801242060664 1106 58-59 0.8598819799513464 1106 60-61 0.9215159661107819 1106 62-63 0.9954282294793089 1106 64-65 0.8786598445447353 1106 66-67 0.8543963424803422 1106 68-69 1.021595438016135 1106 70-71 0.8953064279357292 1106 72-73 0.7911983115627876 1106 74-75 0.755671277912505 1106 76-77 0.7593773437761442 1106 78-79 0.8221595979434433 1106 80-81 0.9265652454778888 1106 82-83 1.0902545574283806 1106 84-85 1.0391057615259562 1106 86-87 0.9872339950545879 1106 88-89 1.0899135783536416 1106 90-91 1.205995305522535 1106 92-93 1.2882730910607556 1106 94-95 1.2720000670785474 1106 96-97 1.7469986542423719 1106 98-99 1.8312685489801659 1106 100-101 1.8906348950091427 1107 1 0.1966016530089263 1107 2 0.1506236229425184 1107 3 0.15095557979984164 1107 4 0.187075146595447 1107 5 0.23326256196970263 1107 6 0.1877159063215288 1107 7 0.20835039536322597 1107 8 0.1387533018207492 1107 9 0.30791158863281964 1107 10-11 0.2571595835490186 1107 12-13 0.2171027568136239 1107 14-15 0.32299351995641956 1107 16-17 0.3101653048957189 1107 18-19 0.32706629647927343 1107 20-21 0.26712027167163654 1107 22-23 0.27882356870026115 1107 24-25 0.3418613529306427 1107 26-27 0.34714063334060796 1107 28-29 0.41491626218070365 1107 30-31 0.3799760913200174 1107 32-33 0.372275432286294 1107 34-35 0.3208215548662423 1107 36-37 0.4626096137120044 1107 38-39 0.4208632921556372 1107 40-41 0.40675972092358137 1107 42-43 0.40816079652424264 1107 44-45 0.33049716567462184 1107 46-47 0.3813392938221156 1107 48-49 0.5036281338384896 1107 50-51 0.538681962669834 1107 52-53 0.5357650945796024 1107 54-55 0.31181273641328744 1107 56-57 0.40449085068441804 1107 58-59 0.5021641036067663 1107 60-61 0.5008186461446584 1107 62-63 0.4795942307702603 1107 64-65 0.4387714877491433 1107 66-67 0.5829468370348323 1107 68-69 0.5796839037592321 1107 70-71 0.6449260910939429 1107 72-73 0.7137135996580355 1107 74-75 0.5964479579239921 1107 76-77 0.5605215723569543 1107 78-79 0.6427033373798317 1107 80-81 0.8090089808100913 1107 82-83 0.8602256229125445 1107 84-85 0.8890490024902142 1107 86-87 0.8352921084719185 1107 88-89 0.8654320583867765 1107 90-91 0.9922037926074054 1107 92-93 0.8783504269958904 1107 94-95 0.9153285251356245 1107 96-97 0.9465192572593182 1107 98-99 0.9171299991012383 1107 100-101 1.098634607716079 1108 1 -0.051060971259445864 1108 2 -0.11781029872592086 1108 3 -0.07274274301414607 1108 4 0.007692028392291661 1108 5 0.005391805959362728 1108 6 0.01671892145083831 1108 7 0.027763014346177783 1108 8 0.10318038659401907 1108 9 0.09006321028125086 1108 10-11 0.16326550500928505 1108 12-13 0.02584421272751314 1108 14-15 0.07483152505969315 1108 16-17 0.13629752335761225 1108 18-19 0.027776392771514224 1108 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-1.17326390975159 2316 60-61 -0.6848797122355705 2316 62-63 -0.7498936821256237 2316 64-65 -0.7817151745197108 2316 66-67 -0.8400009664655457 2316 68-69 -0.9383867120027958 2316 70-71 -0.8838741487241037 2316 72-73 -0.82156696683478 2316 74-75 -0.791999374873356 2316 76-77 -1.088261299549977 2316 78-79 -0.9546202692361199 2316 80-81 -1.0316321888697644 2316 82-83 -1.0768714449855104 2316 84-85 -0.9611831709209007 2316 86-87 -1.0004527189484236 2316 88-89 -1.0152415451369698 2316 90-91 -0.9955875622614485 2316 92-93 -1.161957471194821 2316 94-95 -0.9750715380828829 2316 96-97 -0.9937974021921896 2316 98-99 -0.9860030964755246 2316 100-101 -0.9861553281721065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 13.0 5 38.0 6 87.0 7 218.0 8 558.0 9 1050.0 10 1738.0 11 2805.0 12 4325.0 13 5897.0 14 7472.0 15 10350.0 16 13689.0 17 19089.0 18 17315.0 19 10988.0 20 9822.0 21 10140.0 22 10519.0 23 11724.0 24 13733.0 25 16267.0 26 20002.0 27 25253.0 28 32113.0 29 40604.0 30 50980.0 31 64391.0 32 82291.0 33 109303.0 34 150013.0 35 216113.0 36 338188.0 37 521159.0 38 405010.0 39 33195.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94907140640929 19.51739727848943 15.027746997030272 22.505784318071008 2 15.468585605477077 21.028405600814754 39.66302751390941 23.839981279798756 3 19.679806983636244 24.894067467976488 29.872730278334096 25.55339527005317 4 13.298109956498156 17.360143517090517 37.11736457524543 32.224381951165896 5 13.91384967745481 37.63953469820442 33.77466509013361 14.671950534207163 6 31.578494863827956 36.53673230525689 17.422833330452697 14.461939500462453 7 28.291850195372486 32.89091448149512 20.762974199841434 18.054261123290964 8 26.334890197428802 34.74403922251634 19.690851905574174 19.23021867448068 9 26.382797117075913 17.0764458826861 20.310273751927248 36.23048324831074 10-11 24.774330903343362 26.953211816846455 27.257339106692285 21.015118173117894 12-13 26.086184817997722 24.256733445117014 27.731142154582777 21.92593958230249 14-15 22.851339831864845 25.582766091199993 26.333721626167733 25.232172450767433 16-17 22.09450736751698 28.89664735765197 26.720161988318864 22.288683286512185 18-19 21.943762328497883 27.6139517713373 28.998488339473482 21.44379756069133 20-21 22.901363514589427 26.46148541611315 29.083945902597996 21.553205166699424 22-23 22.56276316121853 26.59385789859244 28.948077496644075 21.895301443544955 24-25 22.577868089594837 26.61524211347379 29.059777165193807 21.74711263173756 26-27 22.38847146229112 26.702964610280546 29.078809865902507 21.829754061525836 28-29 21.986926870603586 27.061173984570004 29.09484821732471 21.8570509275017 30-31 22.354500080780653 26.60881486470404 29.05173707215895 21.984947982356353 32-33 22.293632707834206 26.800780699491856 29.00480757725229 21.90077901542165 34-35 22.61130138504057 26.762589545452126 28.821448008239685 21.804661061267613 36-37 22.610932014171357 26.491635724801917 28.873745668951443 22.02368659207528 38-39 22.30388382194014 26.786336721993038 29.002257358352256 21.907522097714565 40-41 22.518946444716267 26.732724387952278 28.825542023435975 21.922787143895476 42-43 22.369099819448028 26.90047127876356 28.518769162042073 22.21165973974634 44-45 22.560968178463142 26.793026282288924 28.363595188623158 22.282410350624776 46-47 22.8059823067521 27.10943808293898 27.99325844887598 22.09132116143294 48-49 22.88185863347062 27.2094239921186 27.700216290741203 22.208501083669574 50-51 22.551844619921347 27.372040378399028 27.857115633644646 22.21899936803498 52-53 22.878436224897925 27.514040941537477 27.39063842686501 22.216884406699588 54-55 22.606064675303898 27.248205045704555 27.566744201867184 22.57898607712437 56-57 22.694758870181285 27.523160822716918 27.443721898619096 22.3383584084827 58-59 23.067053884461313 27.248664820985635 27.365042883942355 22.3192384106107 60-61 22.82848203364592 27.190397262945172 27.9774999556824 22.003620747726508 62-63 22.628042432856084 27.323607475252153 27.86201743528245 22.186332656609313 64-65 22.562448619626803 27.461482724007347 27.60351968020634 22.372548976159507 66-67 22.580588692543802 27.52448749323054 27.381852443076717 22.513071371148943 68-69 22.47752396066668 27.3221510610543 27.63374553531563 22.566579442963388 70-71 22.647274022948366 27.333026953316637 27.516677694279128 22.50302132945587 72-73 22.68665493883884 27.223613661508654 27.563615784306055 22.526115615346452 74-75 22.59978031810665 27.67561976374779 27.263843626554962 22.4607562915906 76-77 22.646894848708854 27.43671015101638 27.241225574399365 22.675169425875406 78-79 22.64339208483403 27.516815107604724 27.212908932736468 22.62688387482478 80-81 22.74044777098406 27.61497421478754 27.129806251916825 22.514771762311575 82-83 22.66529989620699 27.587158207669336 27.30088538716743 22.44665650895625 84-85 22.60435569073758 27.582037877903183 27.20594899399769 22.607657437361546 86-87 22.601913616262305 27.46577137960613 27.1979785575174 22.734336446614165 88-89 22.62344906869335 27.460500042877534 26.976194438418442 22.93985645001067 90-91 22.622200477208505 27.323488692001973 27.1763996284439 22.877911202345626 92-93 22.71103447175571 27.412743700741338 27.11249410580131 22.763727721701642 94-95 22.51450947858057 27.344650194818776 27.217459591501008 22.923380735099645 96-97 22.397233850147234 27.32871495861921 27.29201968692463 22.982031504308924 98-99 22.37043084057 27.473357349555695 26.871755189646834 23.284456620227473 100-101 22.150471872666397 27.554704655795536 26.940217644204246 23.354605827333824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 220.0 2 155.5 3 212.0 4 219.5 5 222.0 6 371.0 7 781.0 8 1360.0 9 1793.0 10 1830.0 11 1769.5 12 1923.0 13 2109.0 14 2303.0 15 2518.5 16 2661.5 17 2865.5 18 3118.0 19 3278.0 20 3558.5 21 4081.5 22 4895.0 23 5939.0 24 7148.0 25 8978.5 26 11411.0 27 14249.5 28 17119.0 29 20118.0 30 24241.5 31 28925.5 32 33331.0 33 37643.5 34 43137.5 35 48097.5 36 52640.0 37 58172.5 38 61455.0 39 63059.0 40 65744.5 41 70075.5 42 73612.5 43 77501.5 44 82702.5 45 85852.0 46 86809.0 47 91364.0 48 101022.5 49 99949.0 50 90270.0 51 84365.5 52 79746.5 53 76791.0 54 73729.5 55 68067.0 56 61629.5 57 55232.0 58 50380.0 59 45304.5 60 38477.0 61 30788.5 62 23620.0 63 18326.5 64 13574.5 65 9977.0 66 7412.0 67 5505.0 68 3690.5 69 2258.5 70 1533.5 71 989.5 72 676.5 73 493.0 74 342.0 75 234.5 76 158.5 77 80.5 78 41.0 79 23.0 80 11.0 81 7.5 82 7.0 83 4.5 84 3.0 85 4.0 86 2.5 87 1.0 88 0.5 89 0.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01590988358308305 2 0.0032351573859751054 3 0.003102205712578868 4 1.329516733962372E-4 5 0.0013738339584277843 6 5.318066935849488E-4 7 0.0 8 0.0 9 0.0 10-11 1.1079306116353101E-4 12-13 7.75551428144717E-4 14-15 2.21586122327062E-5 16-17 0.0 18-19 0.0 20-21 0.002105068162107089 22-23 0.0 24-25 0.00350106073276758 26-27 0.0018170062030819084 28-29 0.00332379183490593 30-31 0.015488869950661633 32-33 9.306617137736603E-4 34-35 0.0027919851413209813 36-37 0.0034124262838367545 38-39 0.0026811920801574503 40-41 0.004387405222075828 42-43 0.001994275100943558 44-45 6.869169792138921E-4 46-47 2.2158612232706202E-4 48-49 2.4374473455976818E-4 50-51 2.2158612232706202E-4 52-53 9.749789382390727E-4 54-55 0.0028584609780191 56-57 3.766964079560054E-4 58-59 8.863444893082481E-4 60-61 8.420272648428356E-4 62-63 0.0 64-65 1.772688978616496E-4 66-67 4.43172244654124E-5 68-69 2.21586122327062E-5 70-71 8.86344489308248E-5 72-73 1.1079306116353101E-4 74-75 6.425997547484799E-4 76-77 6.64758366981186E-4 78-79 2.659033467924744E-4 80-81 0.005628287507107375 82-83 0.001994275100943558 84-85 0.003434584896069461 86-87 0.002238019835503326 88-89 0.001440309795125903 90-91 1.329516733962372E-4 92-93 1.329516733962372E-4 94-95 1.329516733962372E-4 96-97 0.001661895917452965 98-99 0.0029914126514153368 100-101 0.0010636133871698976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2256459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.926839576823454 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4245187195101 42.741817981619825 2 13.44492313148303 15.038641181943484 3 4.355650597061677 7.307933165836912 4 1.9131629025546542 4.2798861894201625 5 1.0418398135997553 2.913340405997058 6 0.6107458735233171 2.0494251894447277 7 0.4129883843290872 1.6167994582225058 8 0.28567328114043 1.2781443012580491 9 0.21193170865700553 1.0667403604172192 >10 1.167685042057007 12.04616693442507 >50 0.08470223123486852 3.213402056335109 >100 0.04403808579398935 4.350934850920255 >500 0.0010304806561266591 0.3957955901409895 >1k 8.719451705686189E-4 0.8274557224441053 >5k 2.3780322833689605E-4 0.8735166115745325 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7895 0.3498844871544309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6488 0.2875301523315956 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5321 0.2358119513804594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.17274854096617753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.43172244654124E-5 0.0 8 0.0 0.0 0.0 4.43172244654124E-5 0.0 9 0.0 0.0 0.0 4.43172244654124E-5 0.0 10-11 0.0 0.0 0.0 4.43172244654124E-5 0.0 12-13 0.0 0.0 0.0 4.43172244654124E-5 0.0 14-15 0.0 0.0 0.0 4.43172244654124E-5 0.0 16-17 0.0 0.0 0.0 8.86344489308248E-5 0.0 18-19 0.0 0.0 0.0 2.2158612232706202E-4 0.0 20-21 0.0 0.0 0.0 4.210136324214178E-4 0.0 22-23 0.0 0.0 0.0 5.318066935849488E-4 0.0 24-25 0.0 0.0 0.0 7.755514281447169E-4 0.0 26-27 0.0 0.0 0.0 8.641858770755418E-4 0.0 28-29 0.0 0.0 0.0 0.0015732614685221402 0.0 30-31 0.0 0.0 0.0 0.0030800471003461618 0.0 32-33 0.0 0.0 0.0 0.004741943017799127 0.0 34-35 0.0 0.0 0.0 0.005960666690597968 0.0 36-37 0.0 0.0 0.0 0.007799831505912583 0.0 38-39 0.0 0.0 0.0 0.010037851341415908 0.0 40-41 0.0 0.0 0.0 0.014491732400189854 0.0 42-43 0.0 0.0 0.0 0.019898433784970165 0.0 44-45 0.0 0.0 0.0 0.03049025043220373 0.0 46-47 0.0 0.0 0.0 0.05256022821597911 0.0 48-49 0.0 0.0 0.0 0.06689685033054002 0.0 50-51 0.0 0.0 0.0 0.08418056787205086 0.0 52-53 0.0 0.0 0.0 0.10412331888148643 0.0 54-55 0.0 0.0 0.0 0.1319323772335327 0.0 56-57 0.0 0.0 0.0 0.1593868977898557 0.0 58-59 0.0 0.0 0.0 0.19951614454328664 0.0 60-61 0.0 0.0 0.0 0.24354530704967386 0.0 62-63 0.0 0.0 0.0 0.2961941697145838 0.0 64-65 0.0 0.0 0.0 0.3397136841396188 0.0 66-67 0.0 0.0 0.0 0.40858265095886964 0.0 68-69 0.0 0.0 0.0 0.49307343940217835 0.0 70-71 0.0 0.0 0.0 0.549511424758881 0.0 72-73 0.0 0.0 0.0 0.6560943495981979 0.0 74-75 0.0 0.0 0.0 0.7421362408977961 0.0 76-77 0.0 0.0 0.0 0.8135312895115754 0.0 78-79 0.0 0.0 0.0 0.9036282068497589 0.0 80-81 0.0 0.0 0.0 0.9976028813286658 0.0 82-83 0.0 0.0 0.0 1.1192758210984555 0.0 84-85 0.0 0.0 0.0 1.3020400547938165 0.0 86-87 0.0 0.0 0.0 1.5469813544141506 0.0 88-89 0.0 0.0 0.0 1.8373034918870674 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15670 0.0 24.768467 1 GTACATG 9815 0.0 23.038954 1 GGTATCA 11590 0.0 22.830627 1 GAGTACT 8310 0.0 22.404976 12-13 TACATGG 10070 0.0 21.886526 2 GTACTTT 8685 0.0 21.356493 14-15 ACATGGG 9905 0.0 20.812008 3 TCAACGC 18435 0.0 20.792154 4 TATCAAC 18550 0.0 20.689777 2 CAACGCA 18555 0.0 20.657684 5 ATCAACG 18540 0.0 20.649239 3 AGAGTAC 13945 0.0 20.503946 10-11 AACGCAG 18915 0.0 20.339401 6 AGTACTT 8820 0.0 20.328619 12-13 GTACCGT 175 9.9481724E-5 18.998507 6 GTGTAGC 1055 0.0 18.912237 1 CATGGGG 4350 0.0 18.56217 4 ACGCAGA 20835 0.0 18.305496 7 CGCAGAG 20905 0.0 18.22148 8 TACTTTT 9870 0.0 18.02243 14-15 >>END_MODULE