FastQCFastQC Report
Thu 26 May 2016
SRR937034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986235
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.36551566154055287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55460.2792217436506758No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37390.18824560034437013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.1684090754618663No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27310.13749631841146692No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20600.10371381029938552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126200.027.1080631
GTACATG85650.024.4645141
GGTATCA90850.023.482631
TCAACGC145200.023.33184
CAACGCA145700.023.2861045
TATCAAC146350.023.2452742
ATCAACG146300.023.2213293
TACATGG88000.023.1626912
AACGCAG147550.023.154586
GAGTACT65350.022.72140912-13
ACATGGG87300.022.5870343
CATGGGG38550.021.0764314
ACGCAGA165900.020.4502727
CGCAGAG165800.020.4063138
AGAGTAC114800.020.40599310-11
GTACTTT74750.019.67294114-15
GTATAGG7300.019.526471
AGTACTT73050.019.3507412-13
GCAGAGT170500.019.1470329
TCGTATA1300.00755289318.2743112
Produced by FastQC (version 0.11.5)