FastQCFastQC Report
Thu 26 May 2016
SRR937034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986235
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.34784403658177404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57840.2912042129959446No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49670.25007111444516883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.17329268691771116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85000.024.703921
GTATCAA134100.023.806911
TACATGG88750.023.2801112
ACATGGG87850.022.0047683
GAGTACT68150.021.01050212-13
GGTATCA100750.020.7477111
TCAACGC156200.020.3107874
ATCAACG156600.020.2604393
TATCAAC157200.020.2435382
CAACGCA157400.020.1559415
AGAGTAC117100.020.03454810-11
AACGCAG160450.019.80146
AGTACTT72900.019.7066512-13
CATGGGG42050.019.6523554
GTACTTT74450.019.2011714-15
CGCGGTA757.729147E-418.99871358-59
ACGCAGA175900.017.9001717
GTGTAGC8800.017.8153291
CGCAGAG177650.017.6169078
GTATAGG6900.017.2128771
Produced by FastQC (version 0.11.5)