##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986235 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27888240817426 33.0 31.0 34.0 31.0 34.0 2 32.34733150911146 33.0 31.0 34.0 31.0 34.0 3 32.49012881154546 34.0 31.0 34.0 31.0 34.0 4 36.13775409254192 37.0 35.0 37.0 35.0 37.0 5 36.05853688007713 37.0 35.0 37.0 35.0 37.0 6 36.09235160995552 37.0 35.0 37.0 35.0 37.0 7 36.055593623111065 37.0 35.0 37.0 35.0 37.0 8 36.03682595463276 37.0 35.0 37.0 35.0 37.0 9 37.74701684342487 39.0 37.0 39.0 35.0 39.0 10-11 37.64879004750193 39.0 37.0 39.0 35.0 39.0 12-13 37.58016674764064 39.0 37.0 39.0 35.0 39.0 14-15 38.84385206181545 40.0 38.0 41.0 35.0 41.0 16-17 38.77393661877875 40.0 38.0 41.0 35.0 41.0 18-19 38.79953630864424 40.0 38.0 41.0 35.0 41.0 20-21 38.882488728675106 40.0 38.0 41.0 35.0 41.0 22-23 38.81890738004314 40.0 38.0 41.0 35.0 41.0 24-25 38.739640576266154 40.0 38.0 41.0 34.5 41.0 26-27 38.58449654748809 40.0 38.0 41.0 34.0 41.0 28-29 38.4377606375882 40.0 38.0 41.0 34.0 41.0 30-31 38.4240449896412 40.0 38.0 41.0 34.0 41.0 32-33 38.36637759378925 40.0 38.0 41.0 34.0 41.0 34-35 38.25726613416842 40.0 38.0 41.0 34.0 41.0 36-37 38.113871218662446 40.0 38.0 41.0 34.0 41.0 38-39 37.991753996883546 40.0 38.0 41.0 33.0 41.0 40-41 37.79725183575962 40.0 37.0 41.0 33.0 41.0 42-43 37.589266375831656 40.0 37.0 41.0 33.0 41.0 44-45 37.371331942091444 40.0 37.0 41.0 32.5 41.0 46-47 37.05437120985181 40.0 37.0 41.0 31.5 41.0 48-49 36.72117825937011 39.5 36.0 41.0 31.0 41.0 50-51 36.502951060675095 39.0 36.0 41.0 30.5 41.0 52-53 36.28789317477539 39.0 36.0 41.0 30.0 41.0 54-55 36.03448182113395 39.0 35.0 40.0 29.5 41.0 56-57 35.78962811550497 39.0 35.0 40.0 29.0 41.0 58-59 35.457392503908146 38.0 35.0 40.0 28.0 41.0 60-61 35.12377815313898 38.0 34.0 40.0 28.0 41.0 62-63 34.80157433536314 37.5 34.0 40.0 27.5 41.0 64-65 34.399313273605586 37.0 34.0 39.5 26.5 41.0 66-67 33.975139900364255 36.0 33.0 39.0 26.0 40.5 68-69 33.53928387124384 36.0 33.0 39.0 26.0 40.0 70-71 33.14532973187966 35.0 33.0 38.5 25.0 40.0 72-73 32.76024790621452 35.0 32.0 37.5 24.5 40.0 74-75 32.286716073375004 35.0 32.0 37.0 23.5 39.0 76-77 30.808216550408183 33.5 30.0 35.5 21.5 38.0 78-79 31.520947924087533 34.0 31.0 36.0 22.5 38.5 80-81 31.223080098779853 34.0 31.0 36.0 20.0 37.5 82-83 31.036769063076626 34.0 31.0 35.0 20.0 37.0 84-85 30.721232885333308 34.0 31.0 35.0 20.0 36.5 86-87 30.377105931574057 34.0 31.0 35.0 18.5 36.0 88-89 30.02604424954751 34.0 30.0 35.0 17.0 36.0 90-91 29.60052158984209 34.0 30.0 35.0 9.0 35.0 92-93 29.202188311050808 34.0 29.0 35.0 4.5 35.0 94-95 28.751720969573086 34.0 29.0 35.0 2.0 35.0 96-97 28.158814792811526 33.5 29.0 35.0 2.0 35.0 98-99 27.442149846317278 33.0 28.0 35.0 2.0 35.0 100-101 24.26158913723703 30.0 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17265948892202942 1101 2 0.07753063072057387 1101 3 0.11717164173563077 1101 4 0.08661599092118166 1101 5 0.13456600555610976 1101 6 0.07662791159449256 1101 7 0.05512598829032811 1101 8 0.07049525081545482 1101 9 0.12570082264159055 1101 10-11 0.14459737957546537 1101 12-13 0.09679785566334687 1101 14-15 0.19963088494437642 1101 16-17 0.19582415893604832 1101 18-19 0.06209868628446458 1101 20-21 -0.0014795963551890168 1101 22-23 0.12042154163356145 1101 24-25 0.12043932990209782 1101 26-27 0.041577687493052906 1101 28-29 0.13508541585410683 1101 30-31 0.017497122063723225 1101 32-33 0.15650477186679268 1101 34-35 0.13261344860593027 1101 36-37 0.17013921204571147 1101 38-39 0.18281084774937995 1101 40-41 0.34101593677588227 1101 42-43 0.2988273559321897 1101 44-45 0.24282032569818313 1101 46-47 0.22212610829091517 1101 48-49 0.19444717929363264 1101 50-51 -0.03910463249294338 1101 52-53 0.027017491434556007 1101 54-55 0.08172969992772039 1101 56-57 0.009633136763405048 1101 58-59 0.1618573833481065 1101 60-61 -0.04176955637443314 1101 62-63 -0.1437093742610287 1101 64-65 0.11815131578788396 1101 66-67 0.046549698201673095 1101 68-69 0.01099878557703704 1101 70-71 0.11143952917327482 1101 72-73 0.13503868780158967 1101 74-75 0.015507215894572823 1101 76-77 -0.08983849282089196 1101 78-79 0.09370287967766089 1101 80-81 0.535564946332233 1101 82-83 0.4006469351085862 1101 84-85 0.18327265858425434 1101 86-87 0.034690760908041796 1101 88-89 -0.023463567836461863 1101 90-91 -0.07224357887654875 1101 92-93 0.05000268794594831 1101 94-95 0.1385592327774745 1101 96-97 0.09829881144339225 1101 98-99 0.36630960868874496 1101 100-101 0.45742272313137633 1102 1 0.20166493280812858 1102 2 0.08460768264686891 1102 3 0.07416494156812092 1102 4 0.06803349510878576 1102 5 0.046940377415403134 1102 6 0.10742565028795781 1102 7 0.14580438025012654 1102 8 0.13575906991093234 1102 9 0.08714554625967708 1102 10-11 0.08522970788367701 1102 12-13 0.10841741177724629 1102 14-15 0.02128080119228315 1102 16-17 0.15643973682549728 1102 18-19 0.17114077171159892 1102 20-21 -0.028411129018500958 1102 22-23 0.06278335067879226 1102 24-25 0.04361144045486043 1102 26-27 0.022593181630405468 1102 28-29 0.06989697364305414 1102 30-31 0.12777036326974667 1102 32-33 0.05721143015824737 1102 34-35 -0.01063403883125602 1102 36-37 -0.03511099733452738 1102 38-39 -0.043088440357820446 1102 40-41 0.16948850796516268 1102 42-43 0.14496324370438884 1102 44-45 0.1488044128003665 1102 46-47 0.21899063927918405 1102 48-49 0.25473821781959316 1102 50-51 0.09081999062264146 1102 52-53 0.09914793532065858 1102 54-55 0.19424540344530783 1102 56-57 0.29208602621063307 1102 58-59 0.23680294448713113 1102 60-61 0.06693542687514764 1102 62-63 0.19876131250614293 1102 64-65 0.1417202018850361 1102 66-67 0.011564354818084155 1102 68-69 0.07545649076966754 1102 70-71 0.0497110953374289 1102 72-73 0.09545849517177629 1102 74-75 0.12643581723460073 1102 76-77 0.14571531789937708 1102 78-79 0.18513294667933522 1102 80-81 0.25550841366891675 1102 82-83 0.18491410428781663 1102 84-85 0.13072136042680071 1102 86-87 0.03508544265008595 1102 88-89 -0.017912897819712725 1102 90-91 -0.1011705051747036 1102 92-93 0.027320527141927897 1102 94-95 0.2205127503654154 1102 96-97 0.2467012402541151 1102 98-99 0.38993188674569623 1102 100-101 0.753292698005751 1103 1 0.14536988776308135 1103 2 0.07222029525948415 1103 3 0.06402980643299117 1103 4 0.05570241402770648 1103 5 0.05043136840639306 1103 6 0.11815763226994136 1103 7 0.12143501088075936 1103 8 0.1313671780190404 1103 9 0.09122212283625686 1103 10-11 0.07556754572151192 1103 12-13 0.16935772709256725 1103 14-15 0.16227179218327592 1103 16-17 0.1835793764651399 1103 18-19 0.1849301861260173 1103 20-21 0.1291057628733867 1103 22-23 0.10246803536347926 1103 24-25 0.14067225126566996 1103 26-27 0.18814498343220976 1103 28-29 0.16969427094035439 1103 30-31 0.19714536326974752 1103 32-33 0.10256615988798501 1103 34-35 0.11397181702459847 1103 36-37 0.12122346212493085 1103 38-39 0.0993496227052475 1103 40-41 0.08688152598318055 1103 42-43 0.06249139685754557 1103 44-45 0.1828809893769403 1103 46-47 0.2013386122521581 1103 48-49 0.354681911513282 1103 50-51 0.3122614320640835 1103 52-53 0.20490807045579373 1103 54-55 0.196356889931792 1103 56-57 -0.036050235050616664 1103 58-59 0.0038975390817199695 1103 60-61 0.12220006651478599 1103 62-63 0.23781536656019142 1103 64-65 0.1672888955787215 1103 66-67 0.08078732779105735 1103 68-69 0.09668396824714165 1103 70-71 0.10696897822030849 1103 72-73 0.17080196364025113 1103 74-75 -0.047871615197827566 1103 76-77 0.1225002278092866 1103 78-79 0.14007664037303158 1103 80-81 0.4397989542094578 1103 82-83 0.1921438340175463 1103 84-85 0.16467969376013158 1103 86-87 0.2582217039113459 1103 88-89 0.17631007515326402 1103 90-91 0.06174053086133213 1103 92-93 -0.013456499885098339 1103 94-95 0.1123708584735219 1103 96-97 0.3019827717856458 1103 98-99 0.5067562110700194 1103 100-101 0.6730843646724125 1104 1 0.1469710149566268 1104 2 0.13117166368952837 1104 3 0.12720601581772684 1104 4 0.12935853460325575 1104 5 0.033295037762954394 1104 6 0.10189444965605077 1104 7 0.11094656034491379 1104 8 0.1186777113011388 1104 9 0.21290778954561773 1104 10-11 0.1728797868726133 1104 12-13 0.17544841493680252 1104 14-15 0.17191271272466935 1104 16-17 0.14626991060116978 1104 18-19 0.1749391524382986 1104 20-21 0.23472571932273212 1104 22-23 0.07066644704529779 1104 24-25 0.08448229353543013 1104 26-27 0.14759910580102797 1104 28-29 0.12966395626548888 1104 30-31 0.22806953388586493 1104 32-33 0.28140673031622754 1104 34-35 0.25752354410712996 1104 36-37 0.3075380942926458 1104 38-39 0.39157881872590394 1104 40-41 0.26902148584825625 1104 42-43 0.36261628477863894 1104 44-45 0.39042763554918025 1104 46-47 0.24329454923179128 1104 48-49 0.29459801244834694 1104 50-51 0.36189424022769145 1104 52-53 0.25879347244545414 1104 54-55 0.33878035605194157 1104 56-57 0.3647054969847332 1104 58-59 0.27194117513484173 1104 60-61 0.203571485326961 1104 62-63 0.15959069639239232 1104 64-65 0.1665256916164708 1104 66-67 0.19596601358585986 1104 68-69 0.1797515144663535 1104 70-71 0.23415718538481656 1104 72-73 0.3886684082839409 1104 74-75 0.41354975878278566 1104 76-77 0.3942253889894225 1104 78-79 0.5220543526824919 1104 80-81 0.5157671024524859 1104 82-83 0.41363843288181457 1104 84-85 0.3106117632704013 1104 86-87 0.25154180125987935 1104 88-89 0.1851301511534338 1104 90-91 0.2397645264208741 1104 92-93 0.26329406584650883 1104 94-95 0.3866482164001681 1104 96-97 0.4879867852778119 1104 98-99 0.7540748567299005 1104 100-101 0.7173181719393966 1105 1 0.16386511132159853 1105 2 0.09875081799253849 1105 3 0.13997961212962906 1105 4 0.1266893698247884 1105 5 0.19496455787629685 1105 6 0.2007975263022388 1105 7 0.1647576421780741 1105 8 0.14257098162790527 1105 9 0.12626515028434682 1105 10-11 0.09465927620564685 1105 12-13 0.1969992439745809 1105 14-15 0.2600182241069149 1105 16-17 0.2472909215057939 1105 18-19 0.22673128615496552 1105 20-21 0.2628954678656257 1105 22-23 0.22528903065282435 1105 24-25 0.1789120733662486 1105 26-27 0.28427404823215596 1105 28-29 0.2838218357008273 1105 30-31 0.25958450640509767 1105 32-33 0.22075106826276425 1105 34-35 0.21169434481041804 1105 36-37 0.26152868945547425 1105 38-39 0.257334920563963 1105 40-41 0.31466032717970194 1105 42-43 0.3386874809650209 1105 44-45 0.37042320540016505 1105 46-47 0.4553860871857083 1105 48-49 0.6922848747491628 1105 50-51 0.6031147001325294 1105 52-53 0.619367222889629 1105 54-55 0.5806104229195057 1105 56-57 0.40888515798603464 1105 58-59 0.5101468263436715 1105 60-61 0.3736834388842283 1105 62-63 0.3787467068586281 1105 64-65 0.5084134427159341 1105 66-67 0.4491483372913123 1105 68-69 0.4529698792798911 1105 70-71 0.4277131644708234 1105 72-73 0.4093604344772004 1105 74-75 0.28253225507945956 1105 76-77 0.18247834126841056 1105 78-79 0.3259999541444465 1105 80-81 0.621101970955511 1105 82-83 0.4480113129862886 1105 84-85 0.1633570712349801 1105 86-87 0.31323762538296407 1105 88-89 0.22359838098587304 1105 90-91 0.22367593267015096 1105 92-93 0.2840590049088867 1105 94-95 0.42552208175132833 1105 96-97 0.698737510945449 1105 98-99 0.8061579497122651 1105 100-101 0.9036913104692346 1106 1 0.07757940328153268 1106 2 0.03491270948923386 1106 3 0.05365493668769261 1106 4 0.09022419789063463 1106 5 0.12432568732267413 1106 6 0.11562904218644832 1106 7 -0.011367410867492822 1106 8 0.03614340373231073 1106 9 -0.021420827581216884 1106 10-11 0.11330315834731408 1106 12-13 0.04564807307544072 1106 14-15 0.16364292905953448 1106 16-17 0.10310274317005508 1106 18-19 0.1640736048009117 1106 20-21 0.1385681901615854 1106 22-23 0.10266622037131867 1106 24-25 0.05085948828306641 1106 26-27 0.13486685171337598 1106 28-29 0.19360243972407432 1106 30-31 0.06555678347472593 1106 32-33 0.0740687386013974 1106 34-35 0.030578137840286956 1106 36-37 0.030419444344339297 1106 38-39 0.04907328487399809 1106 40-41 0.11874149722821414 1106 42-43 0.06620744819438329 1106 44-45 -0.03010359110398042 1106 46-47 -0.1585060054255436 1106 48-49 0.10990232226078689 1106 50-51 -0.12647137101718897 1106 52-53 -0.2268595683396697 1106 54-55 -0.08405346185483609 1106 56-57 -0.2148328976546665 1106 58-59 -0.08628026683545897 1106 60-61 -0.15631219391548257 1106 62-63 -0.11467695006096079 1106 64-65 -0.2560412061188657 1106 66-67 -0.08296163346887653 1106 68-69 -0.12021517833721163 1106 70-71 -0.030855339508839563 1106 72-73 0.02768732630891435 1106 74-75 0.04627384798130407 1106 76-77 -0.12988161963112788 1106 78-79 -0.0345101719150982 1106 80-81 0.07129355276115845 1106 82-83 0.005422889060877623 1106 84-85 -0.07186252195484855 1106 86-87 -0.009359713523657831 1106 88-89 0.051958380852813235 1106 90-91 0.06416227911030958 1106 92-93 0.12002489512630987 1106 94-95 0.23881619106819585 1106 96-97 0.3716957497709501 1106 98-99 0.6503674650765916 1106 100-101 0.4582234569125312 1107 1 0.007704726150926433 1107 2 -0.0356123836096387 1107 3 -0.0156402327495897 1107 4 0.027239402980690386 1107 5 0.053989990919113495 1107 6 0.06091141302655956 1107 7 0.04208218432017219 1107 8 0.02900130054668182 1107 9 0.011348496251791573 1107 10-11 -0.015930175378670697 1107 12-13 0.048016224681489916 1107 14-15 0.014528947587606922 1107 16-17 0.02263905848506198 1107 18-19 0.05855804967341527 1107 20-21 0.12475528129858304 1107 22-23 0.05780149234151111 1107 24-25 0.20090971305306482 1107 26-27 0.1588585626053245 1107 28-29 0.07158454266024705 1107 30-31 0.06268014793609922 1107 32-33 0.05568467120573928 1107 34-35 0.08167251583036261 1107 36-37 0.08424783292261395 1107 38-39 0.15297688934717968 1107 40-41 0.2658504144172795 1107 42-43 0.0846300878438484 1107 44-45 0.051879424631884774 1107 46-47 0.10560179640176415 1107 48-49 0.06429709934664629 1107 50-51 0.09444359055953555 1107 52-53 0.015221744281056715 1107 54-55 -0.058113505688623945 1107 56-57 -0.10049492109965996 1107 58-59 -0.16096839168577048 1107 60-61 -0.04063723837174393 1107 62-63 0.10401687332015541 1107 64-65 -0.009174162368005057 1107 66-67 -0.12398876870297215 1107 68-69 -0.0375729697130609 1107 70-71 -0.16866118341316394 1107 72-73 -0.08483325037168044 1107 74-75 0.11698706743180054 1107 76-77 0.01887285457708643 1107 78-79 0.151644028868958 1107 80-81 0.27778815022042025 1107 82-83 0.23641236899833729 1107 84-85 0.17896822507422172 1107 86-87 -0.028824381454661818 1107 88-89 -0.07784683850436025 1107 90-91 -0.08704199515735311 1107 92-93 0.04305072906652185 1107 94-95 0.13347871502860897 1107 96-97 0.18675507366632615 1107 98-99 0.42273792870815186 1107 100-101 0.7159539024544017 1108 1 0.011043742561099634 1108 2 0.009463313060727785 1108 3 -0.014486004854717294 1108 4 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-0.9178746907347914 2316 100-101 -1.495045512696592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 7.0 5 41.0 6 67.0 7 165.0 8 447.0 9 795.0 10 1474.0 11 2340.0 12 3570.0 13 5055.0 14 6372.0 15 8848.0 16 11883.0 17 16989.0 18 15404.0 19 9569.0 20 8319.0 21 8537.0 22 8951.0 23 9827.0 24 11437.0 25 13724.0 26 16666.0 27 21178.0 28 26740.0 29 33577.0 30 42882.0 31 54009.0 32 69549.0 33 92553.0 34 129288.0 35 188930.0 36 299484.0 37 471667.0 38 367327.0 39 28560.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.959667948488104 19.512306895850163 15.008943877170752 22.519081278490983 2 15.51405803063206 20.963864157145025 39.67365007308333 23.848427739139584 3 19.64535403922485 24.95890056428959 29.825980079928016 25.569765316557547 4 13.255205462003161 17.421891008649045 37.1728162534972 32.150087275850595 5 13.920932855359085 37.705522213718936 33.73872195593507 14.63482297498691 6 31.457103514941586 36.60407756383308 17.48468836769063 14.454130553534702 7 28.27037082721833 32.95229416458778 20.69966544744202 18.077669560751875 8 26.34784171629584 34.77234017594127 19.6834607857797 19.196357321983193 9 26.343082609140016 17.078258190252605 20.396146877028023 36.18251232357936 10-11 24.73866385397498 27.024873693193403 27.265404144021225 20.971058308810385 12-13 26.07276536217922 24.378604026163085 27.660192016698943 21.88843859495875 14-15 22.847699290365945 25.673825101259418 26.337492794155775 25.14098281421886 16-17 22.026186749946568 28.988787068589872 26.71141132198203 22.273614859481526 18-19 21.882906301780377 27.663780182090704 29.027471700317147 21.425841815811772 20-21 22.89582182687268 26.521231716999722 29.072909465950865 21.510036990176733 22-23 22.524776776161932 26.671541887037538 28.979199339453793 21.82448199734674 24-25 22.485578760334782 26.693454063751016 29.078062748063697 21.7429044278505 26-27 22.37723260645782 26.78553761171057 29.08431005735602 21.752919724475586 28-29 21.96517341542514 27.184176380578922 29.07421645471532 21.776433749280613 30-31 22.287047018583976 26.706498285034534 29.049281366392215 21.95717332998927 32-33 22.179726585765806 26.88371915052585 29.016290767826476 21.92026349588187 34-35 22.53043094732687 26.827669404310683 28.864186541102928 21.777713107259522 36-37 22.5001560986221 26.600564774885644 28.933408327811133 21.965870798681117 38-39 22.283294584425832 26.860255956779127 29.048589493612408 21.807859965182626 40-41 22.47476711934924 26.87876107821079 28.80031439296956 21.84615740947041 42-43 22.290231268239335 27.01556624768641 28.480981747069116 22.213220737005134 44-45 22.492674306198897 26.92706025697829 28.357383090989646 22.222882345833163 46-47 22.777277098364205 27.18972001671542 27.96940373277481 22.063599152145567 48-49 22.856759497290806 27.339343811644156 27.705243888071585 22.098652802993456 50-51 22.491723348502475 27.510655614516356 27.84232585550153 22.155295181479634 52-53 22.85793907909487 27.6305272647219 27.347620326704895 22.163913329478333 54-55 22.552842057353683 27.40803805453826 27.49516413738381 22.543955750724244 56-57 22.66688148060507 27.618007114033478 27.428853371865586 22.28625803349587 58-59 22.965485540055916 27.33649647494652 27.374911326138058 22.32310665885951 60-61 22.70846601736451 27.362472215019874 27.957291055690792 21.971770711924822 62-63 22.599659103524335 27.368635157950965 27.90580765051038 22.125898088014324 64-65 22.520906771583608 27.57020239376178 27.619873126899787 22.289017707754827 66-67 22.51129535890896 27.637102547862412 27.382875158467314 22.46872693476131 68-69 22.432763159319034 27.449789334593355 27.585626167269112 22.531821338818496 70-71 22.58250725589566 27.375388535621926 27.591259141144636 22.45084506733778 72-73 22.62115436372732 27.31402839471019 27.564107889928767 22.500709351633724 74-75 22.529427743457195 27.862635872883125 27.232705811685097 22.375230571974583 76-77 22.581446681160397 27.59202999175287 27.272869164703906 22.553654162382824 78-79 22.57787821408997 27.552622694393737 27.23449604645314 22.635003045063158 80-81 22.81434252678311 27.68381151657434 27.073258776790034 22.428587179852517 82-83 22.5818949696165 27.704065885542146 27.317053276478504 22.396985868362844 84-85 22.552808959347825 27.726088199873526 27.18634327787109 22.53475956290756 86-87 22.51473264744605 27.573700997112628 27.218304984052743 22.69326137138858 88-89 22.522079828738466 27.649604622289175 26.931878026504858 22.8964375224675 90-91 22.581185986785897 27.430217635487868 27.16727981065456 22.821316567071676 92-93 22.595512047078167 27.54086450778448 27.12862593603908 22.734997509098275 94-95 22.447521588100823 27.501539984337093 27.21562021154237 22.835318216019715 96-97 22.287270142199183 27.488964006146322 27.29132808519465 22.93243776645984 98-99 22.260608725925817 27.624764598066538 26.840817317023376 23.27380935898427 100-101 22.00738639410196 27.76014936919107 26.924566997756287 23.30789723895068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 200.0 2 162.5 3 191.5 4 184.0 5 200.5 6 331.5 7 686.5 8 1178.5 9 1541.5 10 1613.0 11 1596.0 12 1751.5 13 1947.0 14 2072.5 15 2280.0 16 2489.5 17 2581.5 18 2700.5 19 2974.0 20 3310.0 21 3733.0 22 4401.5 23 5278.5 24 6361.0 25 7910.0 26 10134.0 27 12805.5 28 15240.0 29 17970.0 30 21884.0 31 26070.0 32 29875.5 33 33518.0 34 38612.5 35 42702.5 36 46211.5 37 51242.0 38 54474.0 39 56158.5 40 58338.0 41 61989.5 42 65346.0 43 68854.5 44 73302.5 45 75888.0 46 76752.0 47 80794.5 48 89205.0 49 87970.5 50 78814.0 51 73369.0 52 69341.0 53 66904.0 54 64380.0 55 59356.5 56 53512.0 57 47933.0 58 43564.5 59 39059.0 60 33178.5 61 26588.5 62 20475.5 63 15817.5 64 11778.5 65 8713.0 66 6348.5 67 4773.0 68 3276.0 69 1907.5 70 1299.0 71 904.0 72 605.0 73 410.0 74 299.0 75 200.5 76 124.0 77 63.5 78 38.5 79 18.0 80 10.5 81 9.5 82 5.0 83 4.0 84 3.5 85 2.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026129838614262664 2 0.007350590438694314 3 0.009968608951105986 4 2.0138603941628256E-4 5 0.003775988239055298 6 0.0 7 0.0 8 2.0138603941628256E-4 9 1.0069301970814128E-4 10-11 0.0 12-13 7.551976478110596E-5 14-15 0.0 16-17 7.551976478110596E-5 18-19 0.004128413808033793 20-21 0.0016110883153302605 22-23 0.0 24-25 0.005135344005115205 26-27 0.006620566045810289 28-29 0.008357520635775727 30-31 0.031542088423575254 32-33 9.062371773732715E-4 34-35 0.004984304475552993 36-37 0.01565776456461597 38-39 0.007929575302016125 40-41 0.009339277577930105 42-43 0.007199550909132101 44-45 0.003775988239055298 46-47 0.0022655929434331787 48-49 0.0018628208646006138 50-51 0.0038011614939823333 52-53 0.00349908243485791 54-55 0.001661434825184331 56-57 0.0010069301970814129 58-59 0.0012083162364976954 60-61 0.0 62-63 7.551976478110596E-5 64-65 0.00785405553723502 66-67 0.0010572767069354835 68-69 0.0014348755308410132 70-71 0.004606705651647463 72-73 0.013417344876109826 74-75 0.007124031144350996 76-77 0.004480839377012286 78-79 0.011755910050925494 80-81 1.0069301970814128E-4 82-83 0.006242967221904759 84-85 9.565836872273422E-4 86-87 0.0 88-89 0.001661434825184331 90-91 0.0012083162364976954 92-93 3.2725231405145914E-4 94-95 6.796778830299537E-4 96-97 3.524255689784945E-4 98-99 2.2655929434331788E-4 100-101 7.803709027380948E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1986235.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.3812123399826 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4275005915361 44.42883852397061 2 13.084503240251678 15.016093175841435 3 4.117821413136361 7.0885675465591405 4 1.745552391527085 4.006476497151204 5 0.9508886628108696 2.728157213621631 6 0.5878563646795505 2.023914653225252 7 0.3788494346936509 1.5217187899927125 8 0.27137505796601785 1.2457463859938522 9 0.2048756247453177 1.0580410554117903 >10 1.1110651820337742 11.758098743189693 >50 0.07703056532272691 3.0209050979348717 >100 0.04048690418884559 4.014868772440788 >500 0.0010533922118933152 0.39838240798697655 >1k 9.656095275688695E-4 1.0508349722372343 >5k 1.7556536864888537E-4 0.6393561644428729 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6909 0.34784403658177404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5784 0.2912042129959446 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4967 0.25007111444516883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3442 0.17329268691771116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.034650985407064E-5 0.0 8 0.0 0.0 0.0 1.0069301970814128E-4 0.0 9 0.0 0.0 0.0 1.5103952956221193E-4 0.0 10-11 0.0 0.0 0.0 2.0138603941628256E-4 0.0 12-13 0.0 0.0 0.0 2.0138603941628256E-4 0.0 14-15 0.0 0.0 0.0 2.0138603941628256E-4 0.0 16-17 0.0 0.0 0.0 2.0138603941628256E-4 0.0 18-19 0.0 0.0 0.0 2.517325492703532E-4 0.0 20-21 0.0 0.0 0.0 4.2794533375960047E-4 0.0 22-23 0.0 0.0 0.0 5.286383534677417E-4 0.0 24-25 0.0 0.0 0.0 6.796778830299537E-4 0.0 26-27 0.0 0.0 0.0 0.0010321034520084482 0.0 28-29 0.0 0.0 0.0 0.0017369545899654372 0.0 30-31 0.0 0.0 0.0 0.0030711371010983092 0.0 32-33 0.0 0.0 0.0 0.004984304475552993 0.0 34-35 0.0 0.0 0.0 0.006696085810591395 0.0 36-37 0.0 0.0 0.0 0.009263757813148997 0.0 38-39 0.0 0.0 0.0 0.011806256560779566 0.0 40-41 0.0 0.0 0.0 0.015632591309688935 0.0 42-43 0.0 0.0 0.0 0.020314816726117502 0.0 44-45 0.0 0.0 0.0 0.03111414308981566 0.0 46-47 0.0 0.0 0.0 0.05475182946630182 0.0 48-49 0.0 0.0 0.0 0.07093823238438554 0.0 50-51 0.0 0.0 0.0 0.08956644103039167 0.0 52-53 0.0 0.0 0.0 0.1127510088181912 0.0 54-55 0.0 0.0 0.0 0.14205267755326031 0.0 56-57 0.0 0.0 0.0 0.1715557323277457 0.0 58-59 0.0 0.0 0.0 0.2146019982529761 0.0 60-61 0.0 0.0 0.0 0.26693719524628257 0.0 62-63 0.0 0.0 0.0 0.32652228965857516 0.0 64-65 0.0 0.0 0.0 0.3714817229582602 0.0 66-67 0.0 0.0 0.0 0.44461002852129783 0.0 68-69 0.0 0.0 0.0 0.5399159716750535 0.0 70-71 0.0 0.0 0.0 0.6023708171490282 0.0 72-73 0.0 0.0 0.0 0.7149204399278031 0.0 74-75 0.0 0.0 0.0 0.8087663342957907 0.0 76-77 0.0 0.0 0.0 0.8883389931201495 0.0 78-79 0.0 0.0 0.0 0.9889564930635095 0.0 80-81 0.0 0.0 0.0 1.0898508988110671 0.0 82-83 0.0 0.0 0.0 1.2170765292123038 0.0 84-85 0.0 0.0 0.0 1.419016380236981 0.0 86-87 0.0 0.0 0.0 1.6825048395582596 0.0 88-89 0.0 0.0 0.0 1.996314635478682 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8500 0.0 24.70392 1 GTATCAA 13410 0.0 23.80691 1 TACATGG 8875 0.0 23.280111 2 ACATGGG 8785 0.0 22.004768 3 GAGTACT 6815 0.0 21.010502 12-13 GGTATCA 10075 0.0 20.747711 1 TCAACGC 15620 0.0 20.310787 4 ATCAACG 15660 0.0 20.260439 3 TATCAAC 15720 0.0 20.243538 2 CAACGCA 15740 0.0 20.155941 5 AGAGTAC 11710 0.0 20.034548 10-11 AACGCAG 16045 0.0 19.8014 6 AGTACTT 7290 0.0 19.70665 12-13 CATGGGG 4205 0.0 19.652355 4 GTACTTT 7445 0.0 19.20117 14-15 CGCGGTA 75 7.729147E-4 18.998713 58-59 ACGCAGA 17590 0.0 17.900171 7 GTGTAGC 880 0.0 17.815329 1 CGCAGAG 17765 0.0 17.616907 8 GTATAGG 690 0.0 17.212877 1 >>END_MODULE