FastQCFastQC Report
Thu 26 May 2016
SRR937033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062455
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.353074370107469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.2717150192367834No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41160.1995679905743398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34120.1654339124974848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26650.1292149404471855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136100.025.6957051
GTACATG88300.024.3230821
GAGTACT68650.023.07956912-13
TACATGG91150.022.9381242
GGTATCA100950.022.9225791
TATCAAC157250.022.240732
ACATGGG90650.022.2259313
TATCTCG1503.0256546E-522.1752595
TCAACGC157950.022.0819974
ATCAACG158350.021.9962083
CAACGCA159550.021.7116415
AACGCAG162750.021.4296936
GTACTTT75350.020.86746614-15
AGAGTAC118150.020.28689610-11
CCGACTA1908.240946E-620.0043569
AGTACTT74850.019.51756912-13
ACGCAGA176850.019.3440427
CATGGGA52700.019.2057344
CGCAGAG176500.019.193968
GTCCTAC4600.018.593261
Produced by FastQC (version 0.11.5)