##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2062455 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.830439452012286 33.0 31.0 34.0 30.0 34.0 2 31.956567294801584 33.0 31.0 34.0 30.0 34.0 3 31.96591004409793 33.0 31.0 34.0 30.0 34.0 4 35.524774601142816 37.0 35.0 37.0 33.0 37.0 5 35.51659890761253 37.0 35.0 37.0 33.0 37.0 6 35.62304001784281 37.0 35.0 37.0 33.0 37.0 7 35.56581258742615 37.0 35.0 37.0 33.0 37.0 8 35.52733126298513 37.0 35.0 37.0 33.0 37.0 9 37.13764373040866 39.0 37.0 39.0 33.0 39.0 10-11 37.01455328722324 39.0 37.0 39.0 33.0 39.0 12-13 36.92489605833825 39.0 37.0 39.0 33.0 39.0 14-15 38.11886877531873 40.0 38.0 41.0 33.0 41.0 16-17 38.041230960190646 40.0 38.0 41.0 33.0 41.0 18-19 37.98186990746465 40.0 38.0 41.0 33.0 41.0 20-21 37.866666909096196 40.0 38.0 41.0 32.5 41.0 22-23 37.73850799168952 40.0 37.5 41.0 32.0 41.0 24-25 37.865652826364695 40.0 38.0 41.0 33.0 41.0 26-27 37.82181938515022 40.0 38.0 41.0 33.0 41.0 28-29 37.733690916892726 40.0 37.5 41.0 32.0 41.0 30-31 37.56497984198443 40.0 37.0 41.0 32.0 41.0 32-33 37.372911166546665 40.0 37.0 41.0 31.5 41.0 34-35 37.1940132027123 40.0 36.5 41.0 31.0 41.0 36-37 37.01913205379026 39.5 36.0 41.0 31.0 41.0 38-39 36.870070134863546 39.0 36.0 41.0 30.5 41.0 40-41 36.69309803122977 39.0 36.0 41.0 30.0 41.0 42-43 36.47090263787574 39.0 35.5 41.0 30.0 41.0 44-45 36.21268924655325 39.0 35.0 41.0 30.0 41.0 46-47 35.8782402525146 39.0 35.0 40.0 28.5 41.0 48-49 35.643627618541984 39.0 35.0 40.0 27.5 41.0 50-51 34.94710187616215 38.0 34.0 39.5 26.5 40.5 52-53 35.14737921554652 38.5 34.5 40.0 27.5 40.5 54-55 35.56215578036854 39.0 35.0 40.5 28.0 41.0 56-57 35.45636971473317 39.0 35.0 41.0 27.0 41.0 58-59 35.21073211294307 38.5 35.0 40.5 26.5 41.0 60-61 34.92529170333414 38.0 34.0 40.0 26.0 41.0 62-63 34.6025602982853 38.0 34.0 40.0 26.0 41.0 64-65 34.268839320130624 37.0 34.0 40.0 25.5 41.0 66-67 33.88511991776791 37.0 34.0 39.5 24.5 41.0 68-69 33.48521713201015 36.0 33.0 39.0 23.0 41.0 70-71 33.08378364618864 36.0 33.0 39.0 22.5 40.5 72-73 32.67505133445336 35.0 32.5 38.5 22.0 40.0 74-75 32.2267004613434 35.0 32.0 37.5 20.5 39.5 76-77 31.793626285179556 35.0 31.5 37.0 20.0 39.0 78-79 31.3604963502234 35.0 31.0 36.5 18.5 39.0 80-81 30.913774361137577 35.0 31.0 36.0 17.0 37.5 82-83 30.50007733502064 34.5 31.0 35.5 12.0 37.0 84-85 30.109523844156598 34.0 30.0 35.0 7.5 37.0 86-87 29.764412072020967 34.0 30.0 35.0 7.0 36.0 88-89 29.4189264735473 34.0 29.5 35.0 2.0 36.0 90-91 29.045021345920276 34.0 29.0 35.0 2.0 35.5 92-93 28.6838275259339 34.0 29.0 35.0 2.0 35.0 94-95 28.27761017816146 34.0 29.0 35.0 2.0 35.0 96-97 27.773922824982847 34.0 28.0 35.0 2.0 35.0 98-99 27.13963456172377 34.0 27.0 35.0 2.0 35.0 100-101 24.614193764227586 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2647029452277927 1101 2 0.22388212317179423 1101 3 0.168286918933088 1101 4 0.19022087297777546 1101 5 0.2412364334797985 1101 6 0.00932732479006404 1101 7 0.04783668963162313 1101 8 0.02867331388299732 1101 9 0.20012830394802705 1101 10-11 0.2095183395666922 1101 12-13 0.2878437338454205 1101 14-15 0.26917384533638966 1101 16-17 0.45679778531842885 1101 18-19 0.406347002806946 1101 20-21 0.3406069362934687 1101 22-23 0.4334437744307138 1101 24-25 0.41226815030531583 1101 26-27 0.3341413911143576 1101 28-29 0.2378045291200621 1101 30-31 0.24090572934263577 1101 32-33 0.32314404869999436 1101 34-35 0.29909608550921263 1101 36-37 0.31891256387670097 1101 38-39 0.2988115048325142 1101 40-41 0.23003593771347397 1101 42-43 0.277889171806315 1101 44-45 0.2731723378220394 1101 46-47 0.28020001988823395 1101 48-49 0.1919936325513092 1101 50-51 0.3771812953133278 1101 52-53 0.44219385834910696 1101 54-55 0.522979482286857 1101 56-57 0.4363753931758083 1101 58-59 0.4740116705955373 1101 60-61 0.6128453575197597 1101 62-63 0.6317022440404543 1101 64-65 0.5393607357028074 1101 66-67 0.592619988318809 1101 68-69 0.559699861858654 1101 70-71 0.5404510663530644 1101 72-73 0.4602396052469686 1101 74-75 0.38340126484013126 1101 76-77 0.48571123311900877 1101 78-79 0.43353740885821423 1101 80-81 0.47493483294165273 1101 82-83 0.3448167188282305 1101 84-85 0.46336996797363383 1101 86-87 0.5835521804384172 1101 88-89 0.6291520487003233 1101 90-91 0.427094129694364 1101 92-93 0.44749027407452147 1101 94-95 0.6250224187371707 1101 96-97 0.4339607755582726 1101 98-99 0.22309737694036258 1101 100-101 0.6408820344097066 1102 1 0.1994217843592203 1102 2 0.25491773063610523 1102 3 0.17286016371619084 1102 4 0.08312956769460556 1102 5 0.056474915356844235 1102 6 0.11466046381254102 1102 7 0.09881448961635186 1102 8 0.11028283233905967 1102 9 0.2097026243805118 1102 10-11 0.224080029793555 1102 12-13 0.1655597485297733 1102 14-15 0.27524328370862605 1102 16-17 0.3362676289675832 1102 18-19 0.3186937227703055 1102 20-21 0.2446614404884997 1102 22-23 0.2311365065107438 1102 24-25 0.04370209502388178 1102 26-27 0.13627082990296202 1102 28-29 0.1794973930538859 1102 30-31 0.17515562004277996 1102 32-33 0.3566050720242302 1102 34-35 0.11898709793817375 1102 36-37 0.1006211461708233 1102 38-39 0.14869127499054002 1102 40-41 0.19891792162730582 1102 42-43 0.21030369237897872 1102 44-45 -0.10086582344209916 1102 46-47 -0.06922086436495078 1102 48-49 -0.013335863142621918 1102 50-51 -0.04318230887545127 1102 52-53 0.11920740459161294 1102 54-55 0.19296643570082495 1102 56-57 0.17916489900166255 1102 58-59 0.24500470316342415 1102 60-61 0.23204327006599357 1102 62-63 0.36456454704047303 1102 64-65 0.4093178485210771 1102 66-67 0.3918965273648851 1102 68-69 0.3344234893647453 1102 70-71 0.3032597950742897 1102 72-73 0.13280950510748113 1102 74-75 0.13352669943717643 1102 76-77 0.25742842060816784 1102 78-79 0.028771414645902382 1102 80-81 0.15905356381417945 1102 82-83 0.17349075542806602 1102 84-85 0.1258181806607439 1102 86-87 0.026030615403268342 1102 88-89 0.20792965992594148 1102 90-91 0.0809897153681014 1102 92-93 -0.036919402606095275 1102 94-95 0.13707559051490747 1102 96-97 0.006288654308299613 1102 98-99 -0.03455083713467744 1102 100-101 0.05707610931654372 1103 1 0.1702792150098773 1103 2 0.2851658528373271 1103 3 0.23838109961637954 1103 4 0.28660278807706874 1103 5 0.2337875976193331 1103 6 0.28741719691424095 1103 7 0.22698713015417127 1103 8 0.13076130791730378 1103 9 0.20305591463105088 1103 10-11 0.224641105882597 1103 12-13 0.3717846844903292 1103 14-15 0.3907649741236412 1103 16-17 0.3854869890635584 1103 18-19 0.4690821092780695 1103 20-21 0.4114537338740476 1103 22-23 0.5112174093631907 1103 24-25 0.2113498195957888 1103 26-27 0.2251500358515628 1103 28-29 0.3221763356768079 1103 30-31 0.4053849736876529 1103 32-33 0.5988407371856468 1103 34-35 0.35132079343861733 1103 36-37 0.4132810561572029 1103 38-39 0.49387737607288784 1103 40-41 0.34858621804600176 1103 42-43 0.3377712388099283 1103 44-45 0.3083948086149704 1103 46-47 0.6069558958238304 1103 48-49 0.7121347654814016 1103 50-51 0.5736598768030987 1103 52-53 0.4413749559269249 1103 54-55 0.7134081286811949 1103 56-57 0.6698233452292754 1103 58-59 0.6590546180918793 1103 60-61 0.7269917932918517 1103 62-63 0.6098487350504769 1103 64-65 0.7041216935286414 1103 66-67 0.6039589559217617 1103 68-69 0.6626827104790962 1103 70-71 0.7120822426449394 1103 72-73 0.44105408863415363 1103 74-75 0.6098574749652457 1103 76-77 0.4456554428022237 1103 78-79 0.4179934532695171 1103 80-81 0.6517556134537088 1103 82-83 0.6440212925294482 1103 84-85 0.5729109407137116 1103 86-87 0.6914166817779375 1103 88-89 0.5423842966081871 1103 90-91 0.5013891555181011 1103 92-93 0.7017574047398654 1103 94-95 0.6206682883204024 1103 96-97 0.4017501533029062 1103 98-99 0.5744465635617395 1103 100-101 0.7753795072740708 1104 1 0.35879176537558877 1104 2 0.2715505619543386 1104 3 0.2735707438946058 1104 4 0.18704529306717887 1104 5 0.355789588640647 1104 6 0.2740223046574215 1104 7 0.23518186047726886 1104 8 0.26105868533437615 1104 9 0.3227084603155461 1104 10-11 0.39536977749366997 1104 12-13 0.45670244687167383 1104 14-15 0.4897120031119897 1104 16-17 0.5398057071753186 1104 18-19 0.40165570021862607 1104 20-21 0.5517477058611178 1104 22-23 0.4846090468727482 1104 24-25 0.4901373056952991 1104 26-27 0.4491070852423533 1104 28-29 0.44825992039918106 1104 30-31 0.3775885279922093 1104 32-33 0.47628433412337046 1104 34-35 0.43761659973695544 1104 36-37 0.4893187830979713 1104 38-39 0.30024924702014744 1104 40-41 0.52242849741382 1104 42-43 0.5422157770125295 1104 44-45 0.5603232965940066 1104 46-47 0.5958074823624386 1104 48-49 0.5197235502652617 1104 50-51 0.5394585104119827 1104 52-53 0.6737010253474622 1104 54-55 0.45741221117899045 1104 56-57 0.6169494737174617 1104 58-59 0.5733759078557981 1104 60-61 0.5655086130019598 1104 62-63 0.8554215253633899 1104 64-65 0.7090436951178418 1104 66-67 0.4993751868641638 1104 68-69 0.5411796802491224 1104 70-71 0.5361125238490771 1104 72-73 0.42226264460123986 1104 74-75 0.4688486640429588 1104 76-77 0.5256960065967782 1104 78-79 0.6586755840038734 1104 80-81 0.702394960334427 1104 82-83 0.6071210977468873 1104 84-85 0.7346519894053323 1104 86-87 0.6902903784977745 1104 88-89 0.631739263965283 1104 90-91 0.5272922223137506 1104 92-93 0.4224255521156195 1104 94-95 0.6844366226432221 1104 96-97 0.6950591363048915 1104 98-99 0.853768471124905 1104 100-101 0.9772058825805807 1105 1 0.20341695629952383 1105 2 0.2615325837219835 1105 3 0.2147434233841743 1105 4 0.3470743076089917 1105 5 0.32495426638462277 1105 6 0.19609837768577876 1105 7 0.18921247233532057 1105 8 0.25064537975750056 1105 9 0.3764858267825204 1105 10-11 0.3635021180084621 1105 12-13 0.3050352269854244 1105 14-15 0.32750679262597515 1105 16-17 0.5249265926748734 1105 18-19 0.46932617960726475 1105 20-21 0.3912421712783001 1105 22-23 0.296838141280368 1105 24-25 0.32280926782475206 1105 26-27 0.33666457304133957 1105 28-29 0.19188076503911589 1105 30-31 0.07814080682161517 1105 32-33 0.3853903214476233 1105 34-35 0.0545699928123895 1105 36-37 0.29951521676932913 1105 38-39 0.2037381951250623 1105 40-41 0.3499415114130855 1105 42-43 0.5681120348611515 1105 44-45 0.2974079712040094 1105 46-47 0.19550337306421994 1105 48-49 0.16297453110673388 1105 50-51 0.29474667182179104 1105 52-53 0.41957536222112424 1105 54-55 0.3913748528502481 1105 56-57 0.3413312297712139 1105 58-59 0.4520560115802539 1105 60-61 0.3347736074290424 1105 62-63 0.3893534380737691 1105 64-65 0.4353900827484125 1105 66-67 0.4553878061679342 1105 68-69 0.42804466069126335 1105 70-71 0.4408515867400382 1105 72-73 0.47875928189672123 1105 74-75 0.5530304312172589 1105 76-77 0.6180454852364612 1105 78-79 0.5796906759464413 1105 80-81 0.4871334292865903 1105 82-83 0.5636270864190855 1105 84-85 0.5388656481441103 1105 86-87 0.4087975112393032 1105 88-89 0.4183125193648607 1105 90-91 0.41362950288285205 1105 92-93 0.38571497469759564 1105 94-95 0.33796685160030293 1105 96-97 0.3714680138785198 1105 98-99 0.11241786273781784 1105 100-101 0.14297072410002087 1106 1 0.19068371434392262 1106 2 0.42491202264581673 1106 3 0.4749964666158668 1106 4 0.488366139871367 1106 5 0.45388315391664236 1106 6 0.3950831970849151 1106 7 0.43467333044647916 1106 8 0.4441516120749611 1106 9 0.4693801514283251 1106 10-11 0.5602815739637066 1106 12-13 0.6302555028761105 1106 14-15 0.7181566631238638 1106 16-17 0.6259213672940973 1106 18-19 0.5057786008302045 1106 20-21 0.70341937854419 1106 22-23 0.6467727560761318 1106 24-25 0.642481879781343 1106 26-27 0.5262959336605562 1106 28-29 0.5613877963832508 1106 30-31 0.5831951940850217 1106 32-33 0.5146404729637268 1106 34-35 0.555427791430489 1106 36-37 0.5802962019275455 1106 38-39 0.44689437766189144 1106 40-41 0.5110722156889622 1106 42-43 0.41266680850197446 1106 44-45 0.5507498187312407 1106 46-47 0.6925599064661654 1106 48-49 0.5676415670892041 1106 50-51 0.4428476698804005 1106 52-53 0.6615575779891287 1106 54-55 0.5028288820917197 1106 56-57 0.5444600941115212 1106 58-59 0.5478605100460712 1106 60-61 0.6485164811891693 1106 62-63 0.5726721935489536 1106 64-65 0.5104744697491057 1106 66-67 0.5545310946609519 1106 68-69 0.5828717058417041 1106 70-71 0.5372229683586198 1106 72-73 0.6343688205720568 1106 74-75 0.7946494839267544 1106 76-77 0.8712381947069225 1106 78-79 0.6248894723332477 1106 80-81 0.5175757113864883 1106 82-83 0.6671390894847384 1106 84-85 0.8044633146600937 1106 86-87 0.8353473013815638 1106 88-89 0.7573128346270863 1106 90-91 0.6567805895863081 1106 92-93 0.44947506105045676 1106 94-95 0.3952929387340731 1106 96-97 0.5367485383733985 1106 98-99 0.6161040753604432 1106 100-101 0.35327927794327607 1107 1 0.2502935789119043 1107 2 0.35299772914266825 1107 3 0.31561885521541555 1107 4 0.18680607031123486 1107 5 0.32406637289030726 1107 6 0.22470741377613734 1107 7 0.19024402111234195 1107 8 0.26513292847914016 1107 9 0.30990766100085665 1107 10-11 0.23102685094809772 1107 12-13 0.319979703353269 1107 14-15 0.23983596399461504 1107 16-17 0.4828256428192219 1107 18-19 0.34130725518725313 1107 20-21 0.45928717865144364 1107 22-23 0.4261616460538775 1107 24-25 0.47634647627375415 1107 26-27 0.3015399910262815 1107 28-29 0.39455327253342176 1107 30-31 0.3397150091021004 1107 32-33 0.4089757496095956 1107 34-35 0.46479582583995693 1107 36-37 0.4681422876554251 1107 38-39 0.2269804948557521 1107 40-41 0.36229889460230424 1107 42-43 0.5555967174002845 1107 44-45 0.5089827792597887 1107 46-47 0.572480363987971 1107 48-49 0.5043115983235609 1107 50-51 0.4051202274804311 1107 52-53 0.6460647905769505 1107 54-55 0.6257181578217583 1107 56-57 0.6039932313912288 1107 58-59 0.6655390117948699 1107 60-61 0.6810252430223258 1107 62-63 0.7083940804032665 1107 64-65 0.6266255527954243 1107 66-67 0.6339785824603084 1107 68-69 0.7157935477955206 1107 70-71 0.5661951478679512 1107 72-73 0.6050097751708918 1107 74-75 0.5612940715459729 1107 76-77 0.5421310241687713 1107 78-79 0.4702270813104974 1107 80-81 0.4618539937999273 1107 82-83 0.4647753767481433 1107 84-85 0.5367151458698594 1107 86-87 0.5872853057326317 1107 88-89 0.6308336349606343 1107 90-91 0.6148849288986504 1107 92-93 0.5216474878688828 1107 94-95 0.6433740883649826 1107 96-97 0.5263085045911211 1107 98-99 0.5444559331185204 1107 100-101 0.5838903256037291 1108 1 -0.09889283972573537 1108 2 0.10003641119996942 1108 3 0.03222359906605732 1108 4 0.0826138252995392 1108 5 0.19562522349025357 1108 6 0.05117456231490536 1108 7 -0.0027335093494613716 1108 8 0.09978951017081528 1108 9 0.14557942504115573 1108 10-11 -0.005699993437048079 1108 12-13 0.09086894313811911 1108 14-15 0.02765008155226667 1108 16-17 0.15793488733272198 1108 18-19 0.27108836506928924 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64-65 -1.3771891475137394 2316 66-67 -1.3806608682953723 2316 68-69 -1.1796667775079825 2316 70-71 -1.1344111696202823 2316 72-73 -1.6396405779665493 2316 74-75 -1.8219192071470083 2316 76-77 -2.096232326602326 2316 78-79 -2.2203467783593034 2316 80-81 -2.3153964073895885 2316 82-83 -1.8466019725904594 2316 84-85 -1.8290876411506396 2316 86-87 -1.9912402887984975 2316 88-89 -1.9771057242241135 2316 90-91 -1.9307222725664808 2316 92-93 -2.29593345268254 2316 94-95 -1.8469965699484838 2316 96-97 -1.9282362699721034 2316 98-99 -1.9472992823451847 2316 100-101 -2.3397637629027592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3644.0 3 1352.0 4 1416.0 5 1543.0 6 1807.0 7 2035.0 8 2576.0 9 3250.0 10 4015.0 11 5032.0 12 6519.0 13 7970.0 14 8974.0 15 10963.0 16 13729.0 17 18430.0 18 16365.0 19 10806.0 20 10651.0 21 11375.0 22 12486.0 23 13734.0 24 16026.0 25 18167.0 26 21193.0 27 25237.0 28 30436.0 29 36658.0 30 44978.0 31 56071.0 32 72117.0 33 94646.0 34 129943.0 35 188899.0 36 287027.0 37 426476.0 38 381640.0 39 64239.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91938913824166 18.646026561526284 14.715822706540479 22.71876159369158 2 15.192868784055527 20.217460772347966 40.76863358931501 23.8210368542815 3 19.397802263878656 24.758693694589258 29.915096064276582 25.928407977255503 4 12.698338031679771 16.71773986662936 38.03599581586103 32.547926285829845 5 13.81807297823964 37.14299297228255 34.21810033816676 14.82083371131105 6 31.153567440827214 36.08557900036572 18.098545162814293 14.66230839599277 7 27.970864793827683 32.71484062440898 21.147126002007653 18.16716857975569 8 26.053767895064126 34.38772372164948 20.181247398867484 19.37726098441891 9 26.216078325628672 17.05463162162529 20.74263466339728 35.986655389348755 10-11 24.545900048908386 26.618214143083584 27.75908654553778 21.076799262470246 12-13 25.920230632700015 24.083588833384702 28.077222571565276 21.918957962350007 14-15 22.790411556450227 25.25376602775878 26.775986361386877 25.17983605440412 16-17 21.91233446540725 28.55469148742971 27.135949978287165 22.397024068875872 18-19 21.82833500271684 27.26359068510675 29.40719064214164 21.500883670034774 20-21 22.860204072726003 26.146030028037938 29.368186301465514 21.625579597770543 22-23 22.469850395117295 26.197195460018165 29.32246972917118 22.01048441569336 24-25 22.3672889526758 26.317885884196862 29.3512604716056 21.963564691521743 26-27 22.173259537783853 26.354320457900897 29.41996310222526 22.052456902089983 28-29 21.855009782221327 26.709246199237302 29.421260997422927 22.014483021118448 30-31 22.154762164507833 26.219747824801026 29.414508437759856 22.21098157293129 32-33 22.027526498837062 26.418447195542775 29.3756354702589 22.178390835361263 34-35 22.44505816857006 26.38948553519254 29.07916976572568 22.08628653051171 36-37 22.359906034313475 26.14360070886395 29.262311177698425 22.23418207912415 38-39 22.155114920278127 26.38748111167705 29.378048405017037 22.079355563027786 40-41 22.33217750985537 26.358860280311635 29.178292175657695 22.1306700341753 42-43 22.161105692807133 26.450269060611546 28.868844800622174 22.519780445959146 44-45 22.31548493093299 26.41601374285529 28.766006118440973 22.502495207770746 46-47 22.532127973701243 26.651757250461223 28.459481540203303 22.35663323563423 48-49 22.59072479958263 26.761694609216434 28.20022924236562 22.447351348835316 50-51 22.25564256103178 26.89599747872676 28.40000969720478 22.44835026303668 52-53 22.580060200305656 27.012608317882435 27.955468453022814 22.45186302878909 54-55 22.406824400232153 26.748998629282163 28.021894635581546 22.82228233490414 56-57 22.489512462859672 27.027081410052524 27.903733815349526 22.579672311738282 58-59 22.85356109663657 26.72716477888863 27.870765287705034 22.548508836769766 60-61 22.611108417377814 26.649098138091542 28.47018037238169 22.269613072148953 62-63 22.412538298510924 26.78483546246952 28.393312312689435 22.409313926330128 64-65 22.281440278816195 26.984354408431976 28.183859459260276 22.550345853491553 66-67 22.39176595465088 26.9758474533123 27.837835740958855 22.794550851077965 68-69 22.22261823711626 26.844912162280117 28.22922667504602 22.703242925557603 70-71 22.359711825498803 26.778351946603674 28.05048170126835 22.811454526629177 72-73 22.425950540426122 26.695969147048572 28.049301487899392 22.828778824625914 74-75 22.307392396076708 27.190024372668088 27.81944149444317 22.68314173681204 76-77 22.386596786932202 26.900367545158684 27.785317932503318 22.9277177354058 78-79 22.38759864351677 26.951399473448628 27.86032858784044 22.800673295194162 80-81 22.426174832760935 27.09451120210104 27.710160505363557 22.769153459774465 82-83 22.38522530072916 26.986422688282502 27.968151469913504 22.660200541074836 84-85 22.390583166879797 26.950585727330434 27.78246547149874 22.87636563429103 86-87 22.387673937463372 26.939294670568977 27.850918400474832 22.822112991492823 88-89 22.45365504402889 26.880232059975985 27.576276212276497 23.089836683718634 90-91 22.406724022502964 26.68976515726299 27.897549974188173 23.00596084604587 92-93 22.5232770971212 26.78743080404559 27.865757665995115 22.823534432838095 94-95 22.327742194521782 26.7438294092075 27.875739827171813 23.052688569098905 96-97 22.164125796704216 26.77813615222233 28.022869042024862 23.03486900904859 98-99 22.18755721201819 26.872959311721996 27.632989525729794 23.30649395053002 100-101 22.2011989413773 26.67058892859923 27.763697137678427 23.364514992345043 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 188.0 1 154.0 2 151.0 3 191.0 4 178.0 5 195.5 6 337.5 7 746.5 8 1344.0 9 1733.5 10 1747.0 11 1660.0 12 1728.0 13 1903.5 14 2078.0 15 2227.5 16 2374.5 17 2478.5 18 2591.5 19 2744.0 20 3049.0 21 3526.5 22 4257.0 23 5287.5 24 6464.5 25 8030.0 26 10357.5 27 13139.0 28 15645.5 29 18528.0 30 22436.0 31 26681.5 32 30737.5 33 34678.5 34 39573.0 35 43869.0 36 48382.5 37 53644.5 38 56687.5 39 58635.0 40 61093.5 41 64567.0 42 67792.0 43 71502.5 44 75865.0 45 78677.0 46 80146.5 47 83723.0 48 91518.0 49 91161.5 50 82439.5 51 76747.0 52 72685.5 53 69835.0 54 67045.0 55 61962.0 56 56444.0 57 50747.0 58 45509.0 59 40631.5 60 34524.5 61 27617.5 62 21222.5 63 16292.0 64 12347.0 65 9168.5 66 6491.5 67 4796.5 68 3406.0 69 2075.5 70 1367.0 71 917.5 72 607.0 73 415.5 74 281.0 75 189.0 76 132.5 77 74.5 78 37.0 79 26.0 80 19.0 81 16.0 82 14.0 83 7.0 84 8.5 85 11.0 86 8.0 87 5.5 88 4.0 89 3.5 90 2.5 91 1.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.5 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02181865786162607 2 0.009600209459115472 3 0.01173358933891891 4 0.018521616229202578 5 0.05071625805169082 6 0.037867492866511025 7 0.016727637693913323 8 0.0413584781243712 9 0.024194467273225353 10-11 0.02116409812577729 12-13 0.03144311027392113 14-15 0.017867056493353795 16-17 0.015054873924521988 18-19 0.015345789362677003 20-21 0.012072990683433094 22-23 0.007563801392030372 24-25 0.001575791956672994 26-27 0.0 28-29 0.0019394362543667621 30-31 0.0 32-33 2.9091543815501427E-4 34-35 4.848590635916905E-5 36-37 0.0 38-39 1.697006722570917E-4 40-41 2.4242953179584523E-5 42-43 7.272885953875357E-5 44-45 7.272885953875357E-5 46-47 0.0 48-49 4.3637315723252143E-4 50-51 2.4242953179584527E-4 52-53 3.394013445141834E-4 54-55 0.0015515490034934095 56-57 3.394013445141834E-4 58-59 0.0010424469867221346 60-61 0.002666724849754298 62-63 0.0018424644416484236 64-65 0.00101820403354255 66-67 0.009115350395523782 68-69 0.011782075245278079 70-71 0.021358041751213967 72-73 0.013866969218722349 74-75 0.014982145064983235 76-77 0.025770259229898347 78-79 0.01735795447658252 80-81 0.039297827104106516 82-83 0.030691578725354007 84-85 0.015418518222215757 86-87 0.012388149074767693 88-89 0.011127515509429297 90-91 0.020145894092234738 92-93 0.02700664984205716 94-95 0.011321459134865973 96-97 0.01774584172745587 98-99 0.024121738413686603 100-101 0.03488560962542213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2062455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.863303065616556 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41910707218706 46.16007665725182 2 12.542261044495376 14.765578259804155 3 3.9466129884801036 6.9693202927080895 4 1.7178938689062881 4.044836297599816 5 0.8728695227710642 2.568999162780662 6 0.5436782460286368 1.9201618419699897 7 0.36456327924291365 1.5021579154869436 8 0.24902557248391877 1.1726774195367657 9 0.18133421233015118 0.9606537626899097 >10 1.0510079977586673 11.39658812550523 >50 0.07419123310567849 3.00207197164269 >100 0.03505863793755105 3.4286326808551295 >500 0.0014873736864970625 0.5609633777487716 >1k 7.436868432485165E-4 0.9205091410643659 >5k 1.6526374294411478E-4 0.6267730933556364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7282 0.353074370107469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5604 0.2717150192367834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4116 0.1995679905743398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3412 0.1654339124974848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2665 0.1292149404471855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.848590635916905E-5 0.0 2 0.0 0.0 0.0 4.848590635916905E-5 0.0 3 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 4 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 5 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 6 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 7 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 8 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 9 0.0 4.848590635916905E-5 0.0 4.848590635916905E-5 0.0 10-11 0.0 4.848590635916905E-5 0.0 7.272885953875357E-5 0.0 12-13 0.0 4.848590635916905E-5 0.0 1.4545771907750713E-4 0.0 14-15 0.0 4.848590635916905E-5 0.0 1.4545771907750713E-4 0.0 16-17 0.0 4.848590635916905E-5 0.0 1.4545771907750713E-4 0.0 18-19 0.0 4.848590635916905E-5 0.0 2.1818657861626071E-4 0.0 20-21 0.0 4.848590635916905E-5 0.0 2.6667248497542974E-4 0.0 22-23 0.0 4.848590635916905E-5 0.0 4.121302040529369E-4 0.0 24-25 0.0 4.848590635916905E-5 0.0 8.000174549262892E-4 0.0 26-27 0.0 4.848590635916905E-5 0.0 0.0012121476589792262 0.0 28-29 0.0 4.848590635916905E-5 0.0 0.001987922160725931 0.0 30-31 0.0 4.848590635916905E-5 0.0 0.0032727986792439106 0.0 32-33 0.0 4.848590635916905E-5 0.0 0.004800104729557736 0.0 34-35 0.0 4.848590635916905E-5 0.0 0.006012252388536962 0.0 36-37 0.0 4.848590635916905E-5 0.0 0.00841230475331583 0.0 38-39 0.0 4.848590635916905E-5 0.0 0.01088508597763345 0.0 40-41 0.0 4.848590635916905E-5 0.0 0.014642743720469052 0.0 42-43 2.4242953179584523E-5 4.848590635916905E-5 0.0 0.0200489222795164 0.0 44-45 4.848590635916905E-5 4.848590635916905E-5 0.0 0.03098249416350902 0.0 46-47 4.848590635916905E-5 4.848590635916905E-5 0.0 0.05427997216908975 0.0 48-49 4.848590635916905E-5 4.848590635916905E-5 0.0 0.06969849039130552 0.0 50-51 4.848590635916905E-5 4.848590635916905E-5 0.0 0.08746857507194097 0.0 52-53 4.848590635916905E-5 4.848590635916905E-5 0.0 0.1082447859468449 0.0 54-55 4.848590635916905E-5 4.848590635916905E-5 0.0 0.1372636009028076 0.0 56-57 4.848590635916905E-5 4.848590635916905E-5 0.0 0.16715516217323528 0.0 58-59 4.848590635916905E-5 4.848590635916905E-5 0.0 0.20701057720047225 0.0 60-61 4.848590635916905E-5 4.848590635916905E-5 0.0 0.25445403657291915 0.0 62-63 4.848590635916905E-5 4.848590635916905E-5 0.0 0.309776455728731 0.0 64-65 4.848590635916905E-5 4.848590635916905E-5 0.0 0.35489259159593783 0.0 66-67 4.848590635916905E-5 4.848590635916905E-5 0.0 0.42973058806131526 0.0 68-69 4.848590635916905E-5 4.848590635916905E-5 0.0 0.5206416624847572 0.0 70-71 4.848590635916905E-5 4.848590635916905E-5 0.0 0.5828975662499303 0.0 72-73 4.848590635916905E-5 4.848590635916905E-5 0.0 0.6919908555580607 0.0 74-75 4.848590635916905E-5 4.848590635916905E-5 0.0 0.7861747286607466 0.0 76-77 4.848590635916905E-5 4.848590635916905E-5 0.0 0.8618369855342298 0.0 78-79 4.848590635916905E-5 4.848590635916905E-5 0.0 0.9571602774363561 0.0 80-81 4.848590635916905E-5 4.848590635916905E-5 0.0 1.0579867197102482 0.0 82-83 4.848590635916905E-5 4.848590635916905E-5 0.0 1.18683801585974 0.0 84-85 4.848590635916905E-5 4.848590635916905E-5 0.0 1.3771209553662989 0.0 86-87 4.848590635916905E-5 4.848590635916905E-5 0.0 1.6339992872571765 0.0 88-89 4.848590635916905E-5 4.848590635916905E-5 0.0 1.9408665886043575 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13610 0.0 25.695705 1 GTACATG 8830 0.0 24.323082 1 GAGTACT 6865 0.0 23.079569 12-13 TACATGG 9115 0.0 22.938124 2 GGTATCA 10095 0.0 22.922579 1 TATCAAC 15725 0.0 22.24073 2 ACATGGG 9065 0.0 22.225931 3 TATCTCG 150 3.0256546E-5 22.175259 5 TCAACGC 15795 0.0 22.081997 4 ATCAACG 15835 0.0 21.996208 3 CAACGCA 15955 0.0 21.711641 5 AACGCAG 16275 0.0 21.429693 6 GTACTTT 7535 0.0 20.867466 14-15 AGAGTAC 11815 0.0 20.286896 10-11 CCGACTA 190 8.240946E-6 20.004356 9 AGTACTT 7485 0.0 19.517569 12-13 ACGCAGA 17685 0.0 19.344042 7 CATGGGA 5270 0.0 19.205734 4 CGCAGAG 17650 0.0 19.19396 8 GTCCTAC 460 0.0 18.59326 1 >>END_MODULE