FastQCFastQC Report
Thu 26 May 2016
SRR937033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062455
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71650.34740151906344624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57570.2791333629097362No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51410.24926604459248805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.16834306687903494No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20770.1007052275079941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145150.023.5979421
GAGTACT72900.022.31323812-13
GGTATCA110050.021.800041
GTACATG91900.021.4530241
GTATACG1353.7936788E-421.114141
TACATGG92800.020.7285142
GTACTTT78100.020.46372214-15
TCAACGC166850.020.3521084
ATCAACG167250.020.3034323
CAACGCA168800.020.1781585
AGAGTAC126150.020.05243910-11
TATCAAC170850.019.9882622
AGTACTT79050.019.91641812-13
AACGCAG171650.019.8984816
ACATGGG91700.019.732643
GTGTAGC9950.018.1433051
ACGCAGA188200.018.0476767
CGCAGAG188350.017.9071968
ACTTTTT92300.017.59890216-17
TACTTTT88500.017.5491114-15
Produced by FastQC (version 0.11.5)