##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937033_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2062455 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.283213936788925 33.0 31.0 34.0 31.0 34.0 2 32.4058808555823 34.0 31.0 34.0 31.0 34.0 3 32.518141244293815 34.0 31.0 34.0 31.0 34.0 4 36.133996135673264 37.0 35.0 37.0 35.0 37.0 5 36.048193536343824 37.0 35.0 37.0 35.0 37.0 6 36.09456885119918 37.0 35.0 37.0 35.0 37.0 7 36.048082018759196 37.0 35.0 37.0 35.0 37.0 8 36.01677612360027 37.0 35.0 37.0 35.0 37.0 9 37.72106494444727 39.0 37.0 39.0 35.0 39.0 10-11 37.63459784577118 39.0 37.0 39.0 35.0 39.0 12-13 37.549373198445544 39.0 37.0 39.0 35.0 39.0 14-15 38.79191133867163 40.0 38.0 41.0 35.0 41.0 16-17 38.71024070828212 40.0 38.0 41.0 35.0 41.0 18-19 38.85680366359509 40.0 38.0 41.0 35.0 41.0 20-21 38.87601474941272 40.0 38.0 41.0 35.0 41.0 22-23 38.79802856304744 40.0 38.0 41.0 35.0 41.0 24-25 38.6911074423442 40.0 38.0 41.0 34.0 41.0 26-27 38.52821443376946 40.0 38.0 41.0 34.0 41.0 28-29 38.46206680873037 40.0 38.0 41.0 34.0 41.0 30-31 38.485054219364784 40.0 38.0 41.0 34.0 41.0 32-33 38.34493552586602 40.0 38.0 41.0 34.0 41.0 34-35 38.20736767590081 40.0 38.0 41.0 34.0 41.0 36-37 38.06907181005161 40.0 38.0 41.0 33.5 41.0 38-39 37.928499530898854 40.0 37.0 41.0 33.0 41.0 40-41 37.77690082935143 40.0 37.0 41.0 33.0 41.0 42-43 37.548827731999 40.0 37.0 41.0 33.0 41.0 44-45 37.30192925421403 40.0 37.0 41.0 32.0 41.0 46-47 36.9802897517764 40.0 36.5 41.0 31.0 41.0 48-49 36.64912761732983 39.0 36.0 41.0 31.0 41.0 50-51 36.375916565452336 39.0 36.0 41.0 30.0 41.0 52-53 36.14417163041133 39.0 35.0 40.0 30.0 41.0 54-55 35.8691273264144 39.0 35.0 40.0 29.0 41.0 56-57 35.578734808759464 38.0 35.0 40.0 28.0 41.0 58-59 35.23555544242177 38.0 34.5 40.0 28.0 41.0 60-61 34.903351588277076 38.0 34.0 40.0 27.0 41.0 62-63 34.540705857824776 37.0 34.0 40.0 26.0 41.0 64-65 34.15262102688301 37.0 33.5 39.0 26.0 41.0 66-67 33.75915207846959 36.0 33.0 39.0 26.0 40.0 68-69 33.44695908516792 36.0 33.0 39.0 25.5 40.0 70-71 32.977509327476234 35.0 32.0 38.0 24.5 40.0 72-73 32.47241200414069 35.0 32.0 37.5 23.5 39.5 74-75 31.961122788133558 35.0 31.0 37.0 22.0 39.0 76-77 30.536234487540334 33.5 29.5 35.5 20.5 37.5 78-79 31.297246970236927 34.0 31.0 36.0 21.5 38.0 80-81 31.06724631567719 34.0 31.0 36.0 20.0 37.0 82-83 30.74859645422567 34.0 31.0 35.0 19.5 37.0 84-85 30.399136466007743 34.0 30.5 35.0 18.0 36.5 86-87 30.06014119095932 34.0 30.0 35.0 17.5 36.0 88-89 29.681499717569594 34.0 30.0 35.0 11.5 36.0 90-91 29.234670089771654 34.0 29.0 35.0 6.5 35.0 92-93 28.772476247966623 34.0 29.0 35.0 2.0 35.0 94-95 28.200995658087088 33.0 29.0 35.0 2.0 35.0 96-97 27.55696948539483 33.0 27.0 35.0 2.0 35.0 98-99 26.754462521606534 33.0 26.0 34.0 2.0 35.0 100-101 23.198149777813335 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04070372947163747 1101 2 0.03144781883668912 1101 3 0.03012421884716332 1101 4 0.08285263996718584 1101 5 0.07876412419323486 1101 6 0.06089000999966032 1101 7 0.11535804890286272 1101 8 0.13820174029050492 1101 9 0.13516490186319174 1101 10-11 0.10997026880696836 1101 12-13 0.11546940470059752 1101 14-15 0.10912100010876458 1101 16-17 0.17514239981025526 1101 18-19 0.16475045755226603 1101 20-21 0.09089554243747244 1101 22-23 0.19307727636310545 1101 24-25 0.1556626176840794 1101 26-27 0.09605585149434859 1101 28-29 0.13780026819502922 1101 30-31 0.22052739865628723 1101 32-33 0.1878067637686769 1101 34-35 0.20095574925429815 1101 36-37 0.18407245978063003 1101 38-39 0.21247071761168712 1101 40-41 0.2977382136171727 1101 42-43 0.3161247527470934 1101 44-45 0.2736822692468408 1101 46-47 0.11134593428736395 1101 48-49 0.060530353795627434 1101 50-51 0.042962908968007696 1101 52-53 -0.02691197695064318 1101 54-55 0.013472002053937615 1101 56-57 0.13978544088802636 1101 58-59 0.14833137745383596 1101 60-61 0.34976209573062533 1101 62-63 0.2575768251229533 1101 64-65 0.1269227918931506 1101 66-67 -0.06797676631541805 1101 68-69 -0.07491274383186663 1101 70-71 -0.14319690304972 1101 72-73 0.14394779050034856 1101 74-75 -0.010756168782290132 1101 76-77 -0.21638277031932063 1101 78-79 -0.0282538650316404 1101 80-81 0.19865423638416502 1101 82-83 0.265749132294566 1101 84-85 0.11322659989598449 1101 86-87 -0.1709160688560729 1101 88-89 -0.11956230246155997 1101 90-91 0.15420975089359246 1101 92-93 0.1567398935151516 1101 94-95 0.04526438860343163 1101 96-97 -0.0289554434081829 1101 98-99 0.03469950417956014 1101 100-101 0.6479474678857855 1102 1 0.18679218254903418 1102 2 0.13109771231270173 1102 3 0.14199730609968242 1102 4 0.06767800308559657 1102 5 0.07449518408955669 1102 6 -0.006624684758804733 1102 7 0.018935103566676048 1102 8 -0.00874202753578146 1102 9 0.13774541942403573 1102 10-11 0.14974865033633478 1102 12-13 0.10179738060605104 1102 14-15 0.21636237582962536 1102 16-17 0.19268016898284657 1102 18-19 0.21110018933389085 1102 20-21 0.16060039812696658 1102 22-23 0.21927489050337812 1102 24-25 0.2362117540417188 1102 26-27 0.12266325335001937 1102 28-29 0.2852400491789311 1102 30-31 0.2426323403977264 1102 32-33 0.22337640686127003 1102 34-35 0.21213157282004858 1102 36-37 0.14919053832385742 1102 38-39 0.1444926261606767 1102 40-41 0.1961745317040382 1102 42-43 0.26562579022826327 1102 44-45 0.25637073342770833 1102 46-47 0.13883866497946684 1102 48-49 0.12436216387064292 1102 50-51 0.15066265011818558 1102 52-53 0.09444087768401488 1102 54-55 0.1975319191942333 1102 56-57 0.2657216028322438 1102 58-59 0.2550802098537801 1102 60-61 0.23250607729885076 1102 62-63 0.35796197699770715 1102 64-65 0.31461528108461323 1102 66-67 0.2580452740873582 1102 68-69 0.11108854694738568 1102 70-71 0.06610520869289616 1102 72-73 0.3240016076677179 1102 74-75 0.21857696330051724 1102 76-77 0.20386129799482688 1102 78-79 0.32694724670404085 1102 80-81 0.4232047190384485 1102 82-83 0.26547952598224 1102 84-85 0.31244172283230043 1102 86-87 0.2233387490121963 1102 88-89 0.1763936028535369 1102 90-91 0.3386756389289012 1102 92-93 0.21532444302625464 1102 94-95 0.02578541928372502 1102 96-97 0.13489631822791281 1102 98-99 0.17422111779242044 1102 100-101 0.5179993072462246 1103 1 0.2112950515916694 1103 2 0.2408134111254725 1103 3 0.2043722924240967 1103 4 0.13760172163647866 1103 5 0.13921652290066788 1103 6 0.086738602813206 1103 7 0.04028693005844275 1103 8 0.08827216866070842 1103 9 0.15894483392052905 1103 10-11 0.20634527916408274 1103 12-13 0.20025411483951672 1103 14-15 0.27253553321010315 1103 16-17 0.25990225287734603 1103 18-19 0.20366972730673893 1103 20-21 0.23015176361017353 1103 22-23 0.2439099882696425 1103 24-25 0.1532611483549431 1103 26-27 0.24232590977037916 1103 28-29 0.22866115144964994 1103 30-31 0.19947069691352937 1103 32-33 0.28978842477344813 1103 34-35 0.32975128602895865 1103 36-37 0.25891019833947837 1103 38-39 0.12450430227763576 1103 40-41 0.2780030795270747 1103 42-43 0.30089841529852634 1103 44-45 0.19607849097192087 1103 46-47 0.19710145117636557 1103 48-49 0.3029724230087609 1103 50-51 0.33158389386043297 1103 52-53 0.29941718591842204 1103 54-55 0.3017353213015923 1103 56-57 0.38456683675070025 1103 58-59 0.28792178814789793 1103 60-61 0.14855790869496843 1103 62-63 0.35065827346166856 1103 64-65 0.28754366630970196 1103 66-67 0.36351852465042356 1103 68-69 0.24667976590382068 1103 70-71 0.16863368219622288 1103 72-73 0.21761586573442315 1103 74-75 0.32547577531692085 1103 76-77 0.20759463736428074 1103 78-79 0.3684152873422093 1103 80-81 0.3861538911470568 1103 82-83 0.4087352951912422 1103 84-85 0.32575042125649745 1103 86-87 0.27309475777965986 1103 88-89 0.10106906716309894 1103 90-91 0.13003210568686185 1103 92-93 0.020445806736354655 1103 94-95 -0.08588428430512707 1103 96-97 0.06666280720633821 1103 98-99 0.01872193078214579 1103 100-101 0.21212842736875714 1104 1 0.22113764839779293 1104 2 0.1609015303938719 1104 3 0.09423305152358097 1104 4 0.025834303464741026 1104 5 0.06082935062307371 1104 6 0.025862171703849413 1104 7 0.07247376862398625 1104 8 0.14474544909435139 1104 9 0.07406182902534653 1104 10-11 0.12130395693305474 1104 12-13 0.1036799372408197 1104 14-15 0.03674466478526739 1104 16-17 0.11490376472754349 1104 18-19 0.20698012672650634 1104 20-21 0.11736151097549197 1104 22-23 0.024012432444195042 1104 24-25 0.1138884506616975 1104 26-27 0.03583791265545244 1104 28-29 0.028143807363598228 1104 30-31 0.16171070639649798 1104 32-33 0.21903674893020764 1104 34-35 0.17178273050387105 1104 36-37 0.30673087382007935 1104 38-39 0.2492448014795272 1104 40-41 0.22895433968685808 1104 42-43 0.1974707847043078 1104 44-45 0.19219579849229262 1104 46-47 0.16489090519251448 1104 48-49 -0.0019161270113770001 1104 50-51 0.10532180031457727 1104 52-53 0.11575591670762009 1104 54-55 0.041678302951446256 1104 56-57 0.14058113754761337 1104 58-59 0.1736923144023521 1104 60-61 0.23314356630962152 1104 62-63 0.24024298177504022 1104 64-65 0.13779977674608546 1104 66-67 0.10484989709897974 1104 68-69 0.1539524182917873 1104 70-71 0.048148463671083164 1104 72-73 0.059554791004821084 1104 74-75 0.19374480227807922 1104 76-77 0.06656121492721923 1104 78-79 0.11167861347290753 1104 80-81 0.38375441913938957 1104 82-83 0.3195262891850277 1104 84-85 0.2704488498363915 1104 86-87 0.2216988830713582 1104 88-89 0.04749020132197046 1104 90-91 0.1728158588725499 1104 92-93 -0.04190450251486766 1104 94-95 0.017777004996702317 1104 96-97 0.05514753149207863 1104 98-99 -0.16172688279215564 1104 100-101 0.20819460321150274 1105 1 0.150067606586191 1105 2 0.2140036600646127 1105 3 0.1938862923653346 1105 4 0.08176669890834631 1105 5 0.047956675093104195 1105 6 0.11412505659514238 1105 7 0.1180337181097002 1105 8 0.0750743226915418 1105 9 0.19350460491834554 1105 10-11 0.1618528111366615 1105 12-13 0.11668930293947 1105 14-15 0.21557931896546023 1105 16-17 0.20887046287437272 1105 18-19 0.11093653835894912 1105 20-21 0.1933353389152117 1105 22-23 0.22102529943308014 1105 24-25 0.06567620642395156 1105 26-27 0.14556094341137538 1105 28-29 0.17244228982892196 1105 30-31 0.2347119970549798 1105 32-33 0.18943721953192494 1105 34-35 0.15133986278204503 1105 36-37 0.1614169034529951 1105 38-39 0.1422458857337432 1105 40-41 0.07770119202188397 1105 42-43 0.09021986620535216 1105 44-45 0.1091228223703169 1105 46-47 0.14401237704866077 1105 48-49 0.027753116479090068 1105 50-51 0.12510375316847444 1105 52-53 0.15513332142257497 1105 54-55 -0.04938093844128133 1105 56-57 0.04611211882716759 1105 58-59 0.02334797733492877 1105 60-61 -0.10740229713837834 1105 62-63 0.002383764645337294 1105 64-65 -0.2055029525922123 1105 66-67 -0.25460228071870716 1105 68-69 -0.016329833216431666 1105 70-71 -0.2571707215628365 1105 72-73 -0.08314089918996359 1105 74-75 0.0074596914389921665 1105 76-77 -0.285744215358271 1105 78-79 0.051839596159382495 1105 80-81 0.0871744534951695 1105 82-83 0.2033617605576019 1105 84-85 0.06835538408980923 1105 86-87 -0.037073929864885 1105 88-89 0.0051805924032883865 1105 90-91 0.156666726318047 1105 92-93 0.15347956105427585 1105 94-95 -0.059856481693589814 1105 96-97 -0.012598769869935467 1105 98-99 0.030944649679444325 1105 100-101 0.43244124872102674 1106 1 0.2534722574287471 1106 2 0.20375061683292017 1106 3 0.1798711652141023 1106 4 0.19454338001245475 1106 5 0.20457201803588987 1106 6 0.23993891780547472 1106 7 0.2227793658150361 1106 8 0.25906063233883714 1106 9 0.3288114375023241 1106 10-11 0.20178998970282436 1106 12-13 0.2533930733126013 1106 14-15 0.4239462470521502 1106 16-17 0.3125635378859357 1106 18-19 0.14144438684827065 1106 20-21 0.2923102384253795 1106 22-23 0.1844734528389509 1106 24-25 0.2526085872063675 1106 26-27 0.32301167689430343 1106 28-29 0.38012490411374245 1106 30-31 0.3157788924377485 1106 32-33 0.16385330497161021 1106 34-35 0.17677328039668083 1106 36-37 0.2039518246329024 1106 38-39 0.3280728103960371 1106 40-41 0.49094530825941973 1106 42-43 0.5069180515451066 1106 44-45 0.3855963561971336 1106 46-47 0.4882534738350728 1106 48-49 0.5306501543730704 1106 50-51 0.26499457097498436 1106 52-53 0.34527437862001165 1106 54-55 0.24728670360563143 1106 56-57 0.4083229359451863 1106 58-59 0.5314151345302918 1106 60-61 0.4207120950351495 1106 62-63 0.47072369160958516 1106 64-65 0.3238388559802061 1106 66-67 0.49479414612822126 1106 68-69 0.5589308696190187 1106 70-71 0.30005718584790486 1106 72-73 0.2983540235481428 1106 74-75 0.5361324737096602 1106 76-77 0.48105221764895845 1106 78-79 0.6064994300306843 1106 80-81 0.6640175824546297 1106 82-83 0.7188737038321413 1106 84-85 0.6518198124550771 1106 86-87 0.5208487094959686 1106 88-89 0.5550464927170111 1106 90-91 0.6541644456495099 1106 92-93 0.7225173454131024 1106 94-95 0.5978437075543361 1106 96-97 0.722161291116997 1106 98-99 0.4889682318374149 1106 100-101 0.5824360849432111 1107 1 0.16601161218009253 1107 2 0.14403228727396566 1107 3 0.12268606113225644 1107 4 0.1364656112820839 1107 5 0.10969210013966091 1107 6 0.1273110538397333 1107 7 0.1697591392100648 1107 8 0.11383269104445759 1107 9 0.19646165371960223 1107 10-11 0.19107021869535146 1107 12-13 0.1585834238037549 1107 14-15 0.2214735125227847 1107 16-17 0.2631552845687395 1107 18-19 0.24131625561765446 1107 20-21 0.10599811938917725 1107 22-23 0.18482936055061572 1107 24-25 0.19089601631436182 1107 26-27 0.33250882250554525 1107 28-29 0.19966020623885328 1107 30-31 0.1947641456533873 1107 32-33 0.23413305110561566 1107 34-35 0.2512036498814538 1107 36-37 0.3070695138252475 1107 38-39 0.3604305675805577 1107 40-41 0.2515954790416757 1107 42-43 0.39699312624563987 1107 44-45 0.37240271478462716 1107 46-47 0.3286107134166443 1107 48-49 0.445464601286254 1107 50-51 0.3574233359796821 1107 52-53 0.35047724601566443 1107 54-55 0.4956151845968719 1107 56-57 0.4205526420785546 1107 58-59 0.5268190697381598 1107 60-61 0.4938612635741535 1107 62-63 0.5494615911928307 1107 64-65 0.4383660822519815 1107 66-67 0.5000102654067504 1107 68-69 0.4279669655207812 1107 70-71 0.46862336330108434 1107 72-73 0.3749247391587289 1107 74-75 0.3307256164059318 1107 76-77 0.44540275957301745 1107 78-79 0.638455510395449 1107 80-81 0.6754168743809146 1107 82-83 0.7565912351600375 1107 84-85 0.6829637417118484 1107 86-87 0.5778844793345961 1107 88-89 0.6115655504836397 1107 90-91 0.8319590561644219 1107 92-93 0.7766596390089049 1107 94-95 0.6360482177380185 1107 96-97 0.6064066509534989 1107 98-99 0.4919353172784291 1107 100-101 0.7447067271450436 1108 1 0.07925690252773165 1108 2 0.020436527860994147 1108 3 0.023021922042353538 1108 4 0.07265309386629326 1108 5 0.04628679560606486 1108 6 0.06794051333798734 1108 7 0.093948513254837 1108 8 0.0932092268006599 1108 9 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-0.41226248782633945 2316 98-99 -0.7277093693404275 2316 100-101 -0.8144148944765774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 14.0 5 46.0 6 79.0 7 185.0 8 433.0 9 880.0 10 1517.0 11 2451.0 12 3639.0 13 5345.0 14 6746.0 15 9302.0 16 12637.0 17 17721.0 18 16294.0 19 10216.0 20 9221.0 21 9435.0 22 10005.0 23 10959.0 24 12690.0 25 15489.0 26 18924.0 27 23763.0 28 29605.0 29 37362.0 30 46991.0 31 59643.0 32 77122.0 33 101913.0 34 140412.0 35 204669.0 36 322910.0 37 488338.0 38 335083.0 39 20408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03595981347165 19.547307439551673 14.993125222793704 22.423607524182966 2 15.511334157635707 20.97602249860596 39.593037069362616 23.919606274395715 3 19.665281907166808 24.96266579779796 29.770001920044685 25.602050374990544 4 13.251036388760939 17.43882275933962 37.161822104778295 32.14831874712114 5 13.922921954660831 37.64576681673055 33.7961313095316 14.635179919077022 6 31.505657093124455 36.590519550729596 17.43965322879772 14.464170127348233 7 28.30539332979386 32.93938534416508 20.72942197526734 18.025799350773713 8 26.37706034798335 34.731521415012686 19.70011466916854 19.19130356783542 9 26.324029529870728 17.116745391654796 20.364720861653158 36.19450421682132 10-11 24.7534263547397 27.019559323028385 27.253652446751996 20.973361875479917 12-13 26.075696197007936 24.351464638016346 27.692846631805303 21.87999253317042 14-15 22.87957797867105 25.64560196464892 26.315071116703155 25.159748939976872 16-17 22.048432571862172 28.992487108809645 26.686158001023053 22.272922318305127 18-19 21.89690508710761 27.659277877901467 29.038027122125204 21.405789912865725 20-21 22.912194302412995 26.503968974529858 29.080584134914346 21.5032525881428 22-23 22.57164302203293 26.636008403748423 28.97133115433233 21.821017419886317 24-25 22.519551020556605 26.69713526481616 29.006137765746587 21.777175948880643 26-27 22.40664393350733 26.7582215767826 29.052129081998174 21.7830054077119 28-29 22.013451975516475 27.153605812122294 29.041774628984584 21.791167583376648 30-31 22.320511210488366 26.682373294401323 29.016184550128273 21.98093094498203 32-33 22.22798691210669 26.868705039459574 28.991996563167316 21.91131148526642 34-35 22.559110380590123 26.81750632135004 28.80918613981881 21.81419715824103 36-37 22.559996353571655 26.57283740614811 28.86979632370198 21.997369916578254 38-39 22.26833543869518 26.802169532949115 29.045895271785643 21.883599756570064 40-41 22.444599493026693 26.862118388158468 28.777474791945057 21.91580732686978 42-43 22.307347311820138 27.020177409931367 28.452984428751172 22.219490849497323 44-45 22.49733933588854 26.92744811401946 28.32134519298603 22.25386735710597 46-47 22.799952192768842 27.129197587625782 28.000685592544205 22.070164627061175 48-49 22.829079735830952 27.347135594769473 27.68339570833181 22.14038896106777 50-51 22.512647145961132 27.44005182505359 27.863821525532128 22.183479503453142 52-53 22.866220111469097 27.577013801513246 27.35439076246512 22.202375324552538 54-55 22.62745444107402 27.357271972629732 27.500964902753317 22.51430868354293 56-57 22.73330832990082 27.576709181391333 27.415124296717853 22.274858191989996 58-59 23.016468508471593 27.317054485887304 27.378122588579657 22.28835441706144 60-61 22.768376429334406 27.29972347897679 27.97485564225559 21.957044449433212 62-63 22.60183538374707 27.401898224741895 27.891510065226626 22.10475632628441 64-65 22.492967720241936 27.61548320968206 27.592331172557344 22.29921789751866 66-67 22.59579893478756 27.605555745463246 27.293953589631137 22.504691730118058 68-69 22.449979987388783 27.470248362444753 27.592821060588392 22.486950589578072 70-71 22.585840239406217 27.468367503282526 27.49442891803673 22.451363339274526 72-73 22.668033969225995 27.278486076059842 27.523145959548206 22.530333995165954 74-75 22.546703454548936 27.785146290499984 27.255752793576782 22.4123974613743 76-77 22.620735967572625 27.54986169395211 27.217539291766368 22.611863046708898 78-79 22.605737110556586 27.56921559657382 27.202921624144906 22.622125668724692 80-81 22.762959098376076 27.654658719984177 27.103144279610476 22.479237902029276 82-83 22.67248500459821 27.654966224431927 27.268925095364672 22.403623675605193 84-85 22.545982819115817 27.71923180616518 27.162327158326754 22.572458216392246 86-87 22.52345122058839 27.580531292699746 27.169837943410624 22.726179543301235 88-89 22.584552979357795 27.569596249508173 26.942671808449127 22.903178962684905 90-91 22.60438775868903 27.44837734870184 27.10145858611816 22.84577630649097 92-93 22.64318265676842 27.53735479063038 27.076520046759857 22.742942505841345 94-95 22.483787525061153 27.459096077247747 27.168907927688117 22.888208470002983 96-97 22.32677383927987 27.487908533968834 27.24081836496711 22.94449926178418 98-99 22.286314001067684 27.62284553921704 26.77992268257429 23.310917777140983 100-101 22.104484830559734 27.65227320386773 26.852788124817422 23.39045384075512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 260.0 1 193.0 2 160.0 3 206.0 4 210.5 5 213.5 6 330.0 7 666.0 8 1172.0 9 1559.0 10 1637.5 11 1621.5 12 1760.0 13 1960.5 14 2120.0 15 2291.0 16 2463.0 17 2638.5 18 2813.0 19 3014.0 20 3355.0 21 3818.0 22 4505.5 23 5467.5 24 6534.0 25 8164.0 26 10571.5 27 13231.0 28 15810.0 29 18810.0 30 22520.5 31 26630.5 32 30867.0 33 34778.5 34 39911.5 35 44294.0 36 48081.5 37 53037.5 38 56516.5 39 58465.0 40 60520.0 41 64119.5 42 67351.5 43 70906.5 44 75754.5 45 78763.0 46 79727.0 47 83643.5 48 92572.0 49 91402.5 50 82063.5 51 76910.5 52 72945.5 53 69861.0 54 66684.5 55 61537.5 56 55643.5 57 50079.0 58 45687.0 59 40958.5 60 34612.5 61 27893.5 62 21474.0 63 16525.0 64 12254.0 65 8953.5 66 6601.0 67 4915.5 68 3409.5 69 2068.0 70 1345.5 71 914.5 72 623.0 73 409.0 74 295.5 75 202.5 76 144.5 77 76.0 78 30.0 79 14.5 80 9.5 81 8.5 82 7.5 83 4.5 84 1.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027636966624726356 2 0.00509102016771275 3 1.4545771907750713E-4 4 2.4242953179584527E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 4.848590635916905E-5 10-11 0.011709346385739325 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.9091543815501427E-4 20-21 9.939610803629657E-4 22-23 0.0020364080670851 24-25 0.010788114164915113 26-27 0.0059395235289982085 28-29 3.394013445141834E-4 30-31 0.002715210756113467 32-33 0.004412217478684383 34-35 0.0 36-37 0.007903202736544554 38-39 0.012606335653383953 40-41 9.212322208242118E-4 42-43 0.0 44-45 0.0 46-47 2.666724849754298E-4 48-49 0.003078855053807235 50-51 0.006400139639410314 52-53 0.0 54-55 0.0034424993515010023 56-57 0.0034667423046805873 58-59 1.697006722570917E-4 60-61 0.0021333798798034384 62-63 0.005187991980431088 64-65 7.272885953875357E-5 66-67 0.002400052364778868 68-69 2.666724849754298E-4 70-71 9.212322208242118E-4 72-73 0.0 74-75 2.4242953179584523E-5 76-77 0.0 78-79 0.0019636792075463467 80-81 0.002084893973444269 82-83 0.011612374573020987 84-85 0.007903202736544554 86-87 0.004315245665966045 88-89 2.666724849754298E-4 90-91 6.060738294896131E-4 92-93 9.69718127183381E-5 94-95 0.0 96-97 0.0024242953179584525 98-99 0.002327323505240114 100-101 1.2121476589792263E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2062455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.85652492600871 #Duplication Level Percentage of deduplicated Percentage of total 1 76.99989850757774 43.779466487962345 2 13.270836448855757 15.090672866867047 3 4.198396874243912 7.1611876958917815 4 1.8411518000171145 4.187259728409556 5 0.9881382454213603 2.8091053390571044 6 0.581387057749403 1.9833388644352679 7 0.37798709386436735 1.50437228368063 8 0.2775934743487036 1.262640023488354 9 0.21357722503599713 1.0928932936998248 >10 1.1254837687855839 11.753263382943665 >50 0.08226932239669008 3.1968327867802215 >100 0.040891263980567576 4.0321957610002 >500 0.0013650958416156374 0.52404411616594 >1k 7.678664109087836E-4 0.7465069798831391 >5k 2.559554703029279E-4 0.8762203897348197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7165 0.34740151906344624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5757 0.2791333629097362 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5141 0.24926604459248805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.16834306687903494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2077 0.1007052275079941 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.848590635916905E-5 0.0 7 0.0 0.0 0.0 4.848590635916905E-5 0.0 8 0.0 0.0 0.0 1.4545771907750713E-4 0.0 9 0.0 0.0 0.0 1.4545771907750713E-4 0.0 10-11 0.0 0.0 0.0 2.1818657861626071E-4 0.0 12-13 0.0 0.0 0.0 2.4242953179584524E-4 0.0 14-15 0.0 0.0 0.0 2.9091543815501427E-4 0.0 16-17 0.0 0.0 0.0 2.9091543815501427E-4 0.0 18-19 0.0 0.0 0.0 3.394013445141833E-4 0.0 20-21 0.0 0.0 0.0 3.6364429769376785E-4 0.0 22-23 0.0 0.0 0.0 5.09102016771275E-4 0.0 24-25 0.0 0.0 0.0 8.485033612854584E-4 0.0 26-27 0.0 0.0 0.0 0.0013091194716975643 0.0 28-29 0.0 0.0 0.0 0.002084893973444269 0.0 30-31 0.0 0.0 0.0 0.00332128458560308 0.0 32-33 0.0 0.0 0.0 0.004969805401814827 0.0 34-35 0.0 0.0 0.0 0.006278924873512392 0.0 36-37 0.0 0.0 0.0 0.00867897723829126 0.0 38-39 0.0 0.0 0.0 0.01120024436896805 0.0 40-41 0.0 0.0 0.0 0.015030630971342405 0.0 42-43 0.0 0.0 0.0 0.02031559476449183 0.0 44-45 0.0 0.0 0.0 0.03132189550802321 0.0 46-47 0.0 0.0 0.0 0.05474058827950186 0.0 48-49 0.0 0.0 0.0 0.07020759240807678 0.0 50-51 0.0 0.0 0.0 0.08773524755691639 0.0 52-53 0.0 0.0 0.0 0.10848721547864075 0.0 54-55 0.0 0.0 0.0 0.1376999740600401 0.0 56-57 0.0 0.0 0.0 0.1677612360027249 0.0 58-59 0.0 0.0 0.0 0.20759240807678228 0.0 60-61 0.0 0.0 0.0 0.2555207265128209 0.0 62-63 0.0 0.0 0.0 0.3109643604345307 0.0 64-65 0.0 0.0 0.0 0.3560804963017375 0.0 66-67 0.0 0.0 0.0 0.43053060551624156 0.0 68-69 0.0 0.0 0.0 0.5214174369865039 0.0 70-71 0.0 0.0 0.0 0.5835521259857791 0.0 72-73 0.0 0.0 0.0 0.6917726689794443 0.0 74-75 0.0 0.0 0.0 0.7849625810017673 0.0 76-77 0.0 0.0 0.0 0.8601157358584793 0.0 78-79 0.0 0.0 0.0 0.9553905418542465 0.0 80-81 0.0 0.0 0.0 1.0563381988940366 0.0 82-83 0.0 0.0 0.0 1.184631907120398 0.0 84-85 0.0 0.0 0.0 1.3751087902523933 0.0 86-87 0.0 0.0 0.0 1.633514428193585 0.0 88-89 0.0 0.0 0.0 1.9409878033702554 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14515 0.0 23.597942 1 GAGTACT 7290 0.0 22.313238 12-13 GGTATCA 11005 0.0 21.80004 1 GTACATG 9190 0.0 21.453024 1 GTATACG 135 3.7936788E-4 21.11414 1 TACATGG 9280 0.0 20.728514 2 GTACTTT 7810 0.0 20.463722 14-15 TCAACGC 16685 0.0 20.352108 4 ATCAACG 16725 0.0 20.303432 3 CAACGCA 16880 0.0 20.178158 5 AGAGTAC 12615 0.0 20.052439 10-11 TATCAAC 17085 0.0 19.988262 2 AGTACTT 7905 0.0 19.916418 12-13 AACGCAG 17165 0.0 19.898481 6 ACATGGG 9170 0.0 19.73264 3 GTGTAGC 995 0.0 18.143305 1 ACGCAGA 18820 0.0 18.047676 7 CGCAGAG 18835 0.0 17.907196 8 ACTTTTT 9230 0.0 17.598902 16-17 TACTTTT 8850 0.0 17.54911 14-15 >>END_MODULE