FastQCFastQC Report
Thu 26 May 2016
SRR937032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113874
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76370.36127981137948617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55950.2646799194275534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41080.19433514012661113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.16046367948136928No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28630.1354385360716864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135800.026.8833081
GTACATG92350.024.1411061
GGTATCA97700.024.1328351
TATCAAC157400.022.9552272
TCAACGC157350.022.902094
ATCAACG157250.022.8864213
CAACGCA158400.022.6897145
GAGTACT69550.022.61586812-13
TACATGG93650.022.5396882
AACGCAG160950.022.356066
ACATGGG93900.021.2645573
GTACTTT76750.020.31018314-15
ACGCAGA176100.019.997577
AGTACTT75850.019.95428812-13
AGAGTAC123150.019.91050310-11
CGCAGAG176000.019.9004528
GCAGAGT183050.018.6138829
GTACCGT1801.2274948E-418.484856
GTATACG1559.5794967E-418.4009741
CATGGGA55250.017.3816814
Produced by FastQC (version 0.11.5)