##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937032_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113874 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76965325274827 33.0 31.0 34.0 30.0 34.0 2 31.864063799450676 33.0 31.0 34.0 30.0 34.0 3 31.887912903039634 33.0 31.0 34.0 30.0 34.0 4 35.47639026734801 37.0 35.0 37.0 33.0 37.0 5 35.477375188871235 37.0 35.0 37.0 33.0 37.0 6 35.54756953347267 37.0 35.0 37.0 33.0 37.0 7 35.48735023941824 37.0 35.0 37.0 33.0 37.0 8 35.45040480179992 37.0 35.0 37.0 33.0 37.0 9 37.07397129630243 39.0 37.0 39.0 33.0 39.0 10-11 36.94591730632952 39.0 37.0 39.0 33.0 39.0 12-13 36.86350298078315 39.0 37.0 39.0 33.0 39.0 14-15 38.024051811981224 40.0 38.0 41.0 33.0 41.0 16-17 37.9585192873369 40.0 38.0 41.0 33.0 41.0 18-19 37.90859554542986 40.0 38.0 41.0 33.0 41.0 20-21 37.839454953322665 40.0 38.0 41.0 32.5 41.0 22-23 37.732605869602445 40.0 37.0 41.0 32.0 41.0 24-25 37.693949355543424 40.0 37.0 41.0 32.0 41.0 26-27 37.72621570632876 40.0 37.0 41.0 32.0 41.0 28-29 37.657564973125176 40.0 37.0 41.0 32.0 41.0 30-31 37.507201706440405 40.0 37.0 41.0 32.0 41.0 32-33 37.33433118530243 40.0 37.0 41.0 31.5 41.0 34-35 37.17331945991104 40.0 36.0 41.0 31.0 41.0 36-37 37.0138539477755 39.5 36.0 41.0 31.0 41.0 38-39 36.85965979996916 39.0 36.0 41.0 30.0 41.0 40-41 36.69161643503823 39.0 36.0 41.0 30.0 41.0 42-43 36.482392517245586 39.0 35.0 41.0 30.0 41.0 44-45 36.239008332568545 39.0 35.0 41.0 30.0 41.0 46-47 35.91635168415904 39.0 35.0 40.0 28.5 41.0 48-49 35.704158336778825 39.0 35.0 40.0 28.0 41.0 50-51 35.022579160347306 38.0 34.0 40.0 27.0 40.5 52-53 35.21281968556309 38.5 34.5 40.0 27.5 40.5 54-55 35.61053047627247 39.0 35.0 40.5 28.0 41.0 56-57 35.49217053618143 39.0 35.0 41.0 27.5 41.0 58-59 35.271553791758635 38.5 35.0 40.5 27.0 41.0 60-61 34.985508360479386 38.0 34.5 40.0 26.0 41.0 62-63 34.683437612648625 38.0 34.0 40.0 26.0 41.0 64-65 34.35462023753544 37.5 34.0 40.0 26.0 41.0 66-67 33.98512092016837 37.0 34.0 39.5 25.0 41.0 68-69 33.59501583348866 36.0 33.0 39.0 24.5 41.0 70-71 33.17942933211724 36.0 33.0 39.0 23.0 40.5 72-73 32.787345414154295 35.0 32.5 38.5 22.5 40.0 74-75 32.390569400068316 35.0 32.0 37.5 22.0 39.5 76-77 31.94570135211465 35.0 32.0 37.0 20.5 39.0 78-79 31.485010696001748 35.0 31.0 36.5 19.5 39.0 80-81 31.048755980725435 35.0 31.0 36.0 18.0 37.5 82-83 30.653300764378578 34.5 31.0 35.5 16.0 37.0 84-85 30.28544487514393 34.0 30.5 35.0 11.0 37.0 86-87 29.95389081846884 34.0 30.0 35.0 7.0 36.0 88-89 29.598083660615536 34.0 30.0 35.0 4.5 36.0 90-91 29.236755123531488 34.0 29.5 35.0 2.0 36.0 92-93 28.867562588877103 34.0 29.0 35.0 2.0 35.0 94-95 28.504790020597255 34.0 29.0 35.0 2.0 35.0 96-97 28.03063025516185 34.0 29.0 35.0 2.0 35.0 98-99 27.479387371243508 34.0 28.0 35.0 2.0 35.0 100-101 25.312821625129978 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31969379101857953 1101 2 0.18655499244336227 1101 3 0.1667457952510354 1101 4 0.24320491799450394 1101 5 0.19343720640539885 1101 6 0.27308805111739787 1101 7 0.22789972019712224 1101 8 0.23887210432184247 1101 9 0.23334917226944185 1101 10-11 0.22999047358451463 1101 12-13 0.32615826416223825 1101 14-15 0.48185706216564483 1101 16-17 0.5576079325251939 1101 18-19 0.5221531295012412 1101 20-21 0.42547014308853903 1101 22-23 0.46180094363162993 1101 24-25 0.4132660643532162 1101 26-27 0.40442318012272693 1101 28-29 0.5012851430982579 1101 30-31 0.4380528406866304 1101 32-33 0.29287402358606585 1101 34-35 0.3738403588654293 1101 36-37 0.4080577964634742 1101 38-39 0.3585764794999804 1101 40-41 0.29399155642072117 1101 42-43 0.32192100764215326 1101 44-45 0.2518527040413616 1101 46-47 0.28292766695422955 1101 48-49 0.34582879780153775 1101 50-51 0.44709523094755355 1101 52-53 0.46467042337179265 1101 54-55 0.4938210195814108 1101 56-57 0.4391394474298522 1101 58-59 0.45814860793920076 1101 60-61 0.5899821844750548 1101 62-63 0.5473259821505394 1101 64-65 0.384807634937367 1101 66-67 0.4421018839424633 1101 68-69 0.504466004634736 1101 70-71 0.4292672296854718 1101 72-73 0.5837872055181066 1101 74-75 0.4791173011369736 1101 76-77 0.5289228735671188 1101 78-79 0.6114338475993897 1101 80-81 0.557506917539559 1101 82-83 0.46644792291910875 1101 84-85 0.34933141579287863 1101 86-87 0.35640079211162856 1101 88-89 0.4110012408735457 1101 90-91 0.5093037246219261 1101 92-93 0.4599215218256596 1101 94-95 0.5352398390088844 1101 96-97 0.5832228991438093 1101 98-99 0.6054994646642662 1101 100-101 0.933514083710488 1102 1 0.12938896108378017 1102 2 0.11492687942648416 1102 3 0.05125304222972815 1102 4 0.16800661241703096 1102 5 0.11175607346797989 1102 6 0.1480800912076603 1102 7 0.05584038772207833 1102 8 0.06165475033427015 1102 9 0.11173005741140685 1102 10-11 0.07297675485310862 1102 12-13 0.05779406022242739 1102 14-15 -0.06625990005593962 1102 16-17 0.2333111732467117 1102 18-19 0.06898543150713721 1102 20-21 0.003251612418658567 1102 22-23 -0.01557180163742089 1102 24-25 0.035945923705071436 1102 26-27 0.09188943702708485 1102 28-29 -0.1317754768056716 1102 30-31 -0.07147136436064017 1102 32-33 -0.11492045846607368 1102 34-35 -0.09696338436864949 1102 36-37 0.010358210378775823 1102 38-39 0.010136967892350413 1102 40-41 -0.14827591038991272 1102 42-43 0.05819812324772755 1102 44-45 0.07040933014710049 1102 46-47 0.09547283078993729 1102 48-49 0.09594335128520726 1102 50-51 0.0557954122950548 1102 52-53 0.2324421331604043 1102 54-55 0.05616164834506776 1102 56-57 0.13975270656298022 1102 58-59 -0.14373408375618624 1102 60-61 -0.052053752787017515 1102 62-63 -0.05519765532056908 1102 64-65 0.11314906661365853 1102 66-67 -0.1104715894852788 1102 68-69 -0.020618439625131657 1102 70-71 0.03568568560141472 1102 72-73 0.05591367810141179 1102 74-75 0.15826545236141953 1102 76-77 0.07708828741320417 1102 78-79 -0.11484942828416322 1102 80-81 0.06604790069223654 1102 82-83 0.26817764591424975 1102 84-85 0.2123302944838521 1102 86-87 0.03692566163832467 1102 88-89 0.2035064667273332 1102 90-91 -0.015257442093194129 1102 92-93 0.22054419441726836 1102 94-95 0.25257756067506776 1102 96-97 -0.05011943234836025 1102 98-99 0.1679549929946269 1102 100-101 0.12654466360721628 1103 1 0.27010315226056747 1103 2 0.29104251851117624 1103 3 0.23279584545571552 1103 4 0.11034788547731722 1103 5 0.10048903308339163 1103 6 0.13228709336657118 1103 7 0.22774138235823926 1103 8 0.19807887036802896 1103 9 0.0718136814221424 1103 10-11 0.20510439959053173 1103 12-13 0.07430841477627581 1103 14-15 0.3574595183330871 1103 16-17 0.3926392139031165 1103 18-19 0.2672697588362283 1103 20-21 0.2801720045236209 1103 22-23 0.3166920380936631 1103 24-25 0.2244694319287177 1103 26-27 0.2833816260947657 1103 28-29 0.32149252704820697 1103 30-31 0.3415536516853237 1103 32-33 0.23810457892706438 1103 34-35 0.3481336314382233 1103 36-37 0.2937359458417106 1103 38-39 0.24738469762000648 1103 40-41 0.1286096146581599 1103 42-43 0.27988038046982666 1103 44-45 0.2571106788617854 1103 46-47 0.19757584800003514 1103 48-49 0.28172321349629215 1103 50-51 0.3033109565982812 1103 52-53 0.3884964062249665 1103 54-55 0.4929366448227839 1103 56-57 0.3363125377210352 1103 58-59 0.3331814340765291 1103 60-61 0.45839764751668355 1103 62-63 0.44910824831258367 1103 64-65 0.3809901482609206 1103 66-67 0.40737376159751904 1103 68-69 0.37314021487649285 1103 70-71 0.4388041880542133 1103 72-73 0.20510874718799244 1103 74-75 0.31591347013365834 1103 76-77 0.40697956623723996 1103 78-79 0.44486823394371555 1103 80-81 0.3703670395755516 1103 82-83 0.448725670659897 1103 84-85 0.41323231947712813 1103 86-87 0.48269066621373824 1103 88-89 0.34049842168763433 1103 90-91 0.36494788929327626 1103 92-93 0.40252212428183043 1103 94-95 0.6333861277244175 1103 96-97 0.648778626339304 1103 98-99 0.7706902424506268 1103 100-101 0.829345164269693 1104 1 0.18077012726974218 1104 2 0.09080383665992997 1104 3 0.06359856593381963 1104 4 0.1814317261513807 1104 5 0.19615165172339033 1104 6 0.0471302273381653 1104 7 0.1114522354602343 1104 8 0.08545986298598507 1104 9 0.18952382064419737 1104 10-11 0.3038187969555963 1104 12-13 0.17338062222375328 1104 14-15 -0.014187788626387032 1104 16-17 0.3066078836230517 1104 18-19 0.35166247169213705 1104 20-21 0.34966363383662724 1104 22-23 0.22241280353074444 1104 24-25 0.216697866547797 1104 26-27 0.22193400442483124 1104 28-29 0.19797418563183555 1104 30-31 0.2937101607737489 1104 32-33 0.062199399662375754 1104 34-35 0.08732333903748923 1104 36-37 0.03876053912156152 1104 38-39 0.23262486253168646 1104 40-41 0.1986692348477277 1104 42-43 0.2166956251709351 1104 44-45 0.1212278286477968 1104 46-47 0.12809011612420562 1104 48-49 0.19693972291729978 1104 50-51 0.2830498760872402 1104 52-53 0.2261258453032795 1104 54-55 0.4025585166190737 1104 56-57 0.41093870787891973 1104 58-59 0.3503741070232351 1104 60-61 0.3690766107588246 1104 62-63 0.30130445392083516 1104 64-65 0.2620165072914773 1104 66-67 0.07467419328991554 1104 68-69 0.15972646499202625 1104 70-71 0.07414350725406393 1104 72-73 0.1489709459304649 1104 74-75 0.2796576698591622 1104 76-77 0.294366359613079 1104 78-79 0.33304605733693293 1104 80-81 0.1709254785374661 1104 82-83 0.09963122697384819 1104 84-85 0.08022318405688722 1104 86-87 0.25119092666776766 1104 88-89 0.09807527496210611 1104 90-91 0.18394655086507328 1104 92-93 0.20832599524358386 1104 94-95 0.3750221116044976 1104 96-97 0.2982747918449782 1104 98-99 0.2698011796249382 1104 100-101 0.3424867812657588 1105 1 0.15028024274368335 1105 2 0.17921829686745383 1105 3 0.14423855533965124 1105 4 0.15262549824598892 1105 5 0.1720116054738483 1105 6 0.19958839515496152 1105 7 0.21407054429941041 1105 8 0.23221554454625704 1105 9 0.22511797902618724 1105 10-11 0.2109772942993473 1105 12-13 0.19683000695823694 1105 14-15 0.33160583913100083 1105 16-17 0.3625393896566038 1105 18-19 0.2815383608381481 1105 20-21 0.3854950574572129 1105 22-23 0.3485651380166388 1105 24-25 0.30324096095291253 1105 26-27 0.2874664759269834 1105 28-29 0.3622582401168515 1105 30-31 0.3154251876277243 1105 32-33 0.22004845441182397 1105 34-35 0.08439380566814947 1105 36-37 0.14778865323042822 1105 38-39 0.3748131622217272 1105 40-41 0.22439351327614787 1105 42-43 0.3088800936364109 1105 44-45 0.25859867577735685 1105 46-47 0.22155325578775376 1105 48-49 0.2084347500294612 1105 50-51 0.09161992982016187 1105 52-53 0.1657753132419657 1105 54-55 0.11869871611759919 1105 56-57 0.22758129752021006 1105 58-59 0.33235902282581975 1105 60-61 0.36916583248303425 1105 62-63 0.3789621265102028 1105 64-65 0.3430556519955843 1105 66-67 0.34667021131499354 1105 68-69 0.45483215889967 1105 70-71 0.31781626070934266 1105 72-73 0.3718203692270592 1105 74-75 0.4954364016488739 1105 76-77 0.45923863199982407 1105 78-79 0.5760340835108657 1105 80-81 0.38604124190270994 1105 82-83 0.2888712327873151 1105 84-85 0.4280549608164286 1105 86-87 0.5600383277440919 1105 88-89 0.6004680204551072 1105 90-91 0.5499661292554698 1105 92-93 0.5149573244161516 1105 94-95 0.6091988418292367 1105 96-97 0.48365994026953274 1105 98-99 0.5754738697817317 1105 100-101 0.7672564184779418 1106 1 0.4137308986920516 1106 2 0.3201457563371548 1106 3 0.32145018177360285 1106 4 0.32253041853212494 1106 5 0.2758338221353469 1106 6 0.30919072509885837 1106 7 0.3352478714846683 1106 8 0.3708574590344682 1106 9 0.4693133203548001 1106 10-11 0.46269004113256074 1106 12-13 0.4673758008343114 1106 14-15 0.4692941761179128 1106 16-17 0.5313869032738623 1106 18-19 0.6555125590727968 1106 20-21 0.5370417645086434 1106 22-23 0.4192089396759897 1106 24-25 0.38270285651728386 1106 26-27 0.3548059903540164 1106 28-29 0.3610739794022493 1106 30-31 0.5021917040679185 1106 32-33 0.5608086142479181 1106 34-35 0.5203605334159107 1106 36-37 0.4265929930289687 1106 38-39 0.4113112867802471 1106 40-41 0.4802555169271159 1106 42-43 0.5289803343984474 1106 44-45 0.5055467933415159 1106 46-47 0.5656817815265285 1106 48-49 0.8539439430378906 1106 50-51 0.8315306888678577 1106 52-53 0.7645101179084506 1106 54-55 0.7429455202210633 1106 56-57 0.7827743198525994 1106 58-59 0.7885305155938411 1106 60-61 1.0148829010424336 1106 62-63 0.8445199497990501 1106 64-65 0.7242798630459575 1106 66-67 0.7087318589057716 1106 68-69 0.748291399217706 1106 70-71 0.8681021601870711 1106 72-73 0.8716483342883308 1106 74-75 0.9731524466872727 1106 76-77 0.9833235234223139 1106 78-79 1.0394751385194674 1106 80-81 1.0766976594575688 1106 82-83 0.967940706230074 1106 84-85 1.0141903783791122 1106 86-87 1.2816786287531023 1106 88-89 1.2530860359886447 1106 90-91 1.2201336634821658 1106 92-93 1.3493923764480336 1106 94-95 1.2816456217783063 1106 96-97 1.404828622264688 1106 98-99 1.6894137826694937 1106 100-101 1.9282687398287806 1107 1 0.2876544646962884 1107 2 0.2950223065539248 1107 3 0.2816265032386589 1107 4 0.24528058681434572 1107 5 0.28360399976943285 1107 6 0.23283263734546011 1107 7 0.39196353502111947 1107 8 0.29676867048429045 1107 9 0.3345691256827479 1107 10-11 0.38473146214922593 1107 12-13 0.4278723373269173 1107 14-15 0.27188289623408934 1107 16-17 0.48110006441512354 1107 18-19 0.5095002288089461 1107 20-21 0.6276991699307715 1107 22-23 0.6449910140807873 1107 24-25 0.569313594160981 1107 26-27 0.6812933552903147 1107 28-29 0.6288864366309994 1107 30-31 0.6411109561614623 1107 32-33 0.7215705718618182 1107 34-35 0.5554515225977283 1107 36-37 0.5335964296766136 1107 38-39 0.6402881542224321 1107 40-41 0.7309438274203046 1107 42-43 0.7221287371303831 1107 44-45 0.8042309221207447 1107 46-47 0.7407945574755033 1107 48-49 0.7673020714312742 1107 50-51 0.6774110326167033 1107 52-53 0.8093478125657967 1107 54-55 0.7605214947981906 1107 56-57 0.8055921513685789 1107 58-59 0.9842568711824669 1107 60-61 0.9384763489431478 1107 62-63 0.9126753010335875 1107 64-65 1.044194995553319 1107 66-67 0.9869695224525046 1107 68-69 0.9414673623969492 1107 70-71 0.911962977125448 1107 72-73 0.8544849489498318 1107 74-75 0.7625552444930861 1107 76-77 0.8754989956518955 1107 78-79 0.7943293974727546 1107 80-81 0.9446303316204485 1107 82-83 0.9050597939886131 1107 84-85 0.863574402381925 1107 86-87 0.9678223569529045 1107 88-89 0.8094650182981056 1107 90-91 0.851365185720411 1107 92-93 0.886323492613446 1107 94-95 0.8569697256316751 1107 96-97 0.7650644556108652 1107 98-99 0.9617871435819616 1107 100-101 1.1713609939884222 1108 1 0.17158500240400798 1108 2 0.1730998932810266 1108 3 0.10152818435047095 1108 4 0.05935447895660673 1108 5 0.14731185145252113 1108 6 0.09382833597760509 1108 7 0.04178338170208917 1108 8 0.07263237878132145 1108 9 0.05957172538610678 1108 10-11 -0.007761173509088337 1108 12-13 0.10783783197086194 1108 14-15 0.16784198859359378 1108 16-17 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2316 64-65 -2.8191475139366773 2316 66-67 -2.6095111169048906 2316 68-69 -2.7587119252512196 2316 70-71 -2.347332657333851 2316 72-73 -2.8444453072380718 2316 74-75 -3.311161408975895 2316 76-77 -3.370202259863788 2316 78-79 -3.672567557322605 2316 80-81 -3.597347583162904 2316 82-83 -4.202027351554229 2316 84-85 -3.9557964577861533 2316 86-87 -4.207041065026512 2316 88-89 -3.973764709392949 2316 90-91 -4.560178563456777 2316 92-93 -4.765288207717685 2316 94-95 -4.703942445034794 2316 96-97 -4.669126585290169 2316 98-99 -5.044758318346815 2316 100-101 -5.365381511877398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3763.0 3 1477.0 4 1380.0 5 1500.0 6 1732.0 7 2102.0 8 2575.0 9 3196.0 10 3979.0 11 5120.0 12 6645.0 13 8107.0 14 9064.0 15 10945.0 16 13877.0 17 18220.0 18 16120.0 19 10597.0 20 10803.0 21 11552.0 22 12554.0 23 13940.0 24 16098.0 25 18574.0 26 21671.0 27 25883.0 28 30900.0 29 37182.0 30 46281.0 31 57641.0 32 72685.0 33 96271.0 34 132188.0 35 189402.0 36 290779.0 37 435319.0 38 402656.0 39 71066.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.903640547783404 18.679841811192752 14.685938809495303 22.730578831528543 2 15.197380406519645 20.147257494777136 40.82075232869841 23.834609770004803 3 19.345292875991944 24.75438901146634 29.954533044573132 25.94578506796858 4 12.695806668099118 16.74417371928785 37.99006685515726 32.56995275745577 5 13.792638111756492 37.18592132034617 34.20059651570056 14.82084405219678 6 31.208102797590602 36.02329454855091 18.117871703810486 14.650730950047997 7 28.050987250820636 32.742505390060515 21.16311707613771 18.043390282981136 8 25.990285695618148 34.3836567883898 20.253884325067006 19.372173190925047 9 26.22327651542945 17.08423652292796 20.71291660137823 35.97957036026436 10-11 24.50236900638544 26.639386266745596 27.786195073006393 21.072049653862567 12-13 25.890085294811023 24.06863538716876 28.06321416297188 21.978065155048338 14-15 22.80999555751947 25.213786979153475 26.74304640557883 25.233171057748223 16-17 21.921956388855428 28.573338668985198 27.120170254939875 22.384534687219496 18-19 21.81082990453808 27.286275707089175 29.36551608249936 21.537378305873386 20-21 22.83589793471076 26.213319266069373 29.28338602640736 21.667396772812502 22-23 22.40945411008658 26.244880285639244 29.31424812661636 22.031417477657815 24-25 22.40118730413456 26.304847054637943 29.297424984482156 21.996540656745342 26-27 22.16090607654305 26.396443022267302 29.358927475746484 22.083723425443168 28-29 21.874167645582485 26.749168414364537 29.341714398176116 22.034949541876863 30-31 22.154914968379995 26.314752415408943 29.314247394173215 22.216085222037847 32-33 22.065551899590538 26.43282420537955 29.309424741395013 22.1921991536349 34-35 22.483799987983343 26.415753550708626 29.032758631293547 22.06768783001448 36-37 22.369017095685074 26.17432153399657 29.18865100660187 22.26801036371649 38-39 22.14790517306429 26.40544733772628 29.35274544279134 22.093902046418087 40-41 22.29472783821402 26.419355388119254 29.13352188052643 22.152394893140297 42-43 22.157664118930516 26.456377509021788 28.845589779787034 22.54036859226066 44-45 22.332446393939705 26.44615314381735 28.726475980610488 22.494924481632452 46-47 22.524131621175652 26.674642732791376 28.457548972856777 22.343676673176198 48-49 22.61306021560059 26.774210716165314 28.16713480654489 22.445594261689205 50-51 22.266297381486115 26.88926908641656 28.38064947546224 22.46378405663508 52-53 22.552395073246302 27.08735220696926 27.892636907033058 22.46761581275138 54-55 22.393330817880848 26.78942705641198 28.032943753614976 22.7842983720922 56-57 22.48320504453067 27.09088590832328 27.87675191155796 22.549157135588086 58-59 22.83244001724894 26.747513368990283 27.86349023194994 22.55655638181084 60-61 22.56691363024155 26.705536503569157 28.44800873495073 22.279541131238567 62-63 22.42342293965737 26.761561576621535 28.393437674681238 22.421577809039864 64-65 22.280368897605108 27.006523523975527 28.150097584387257 22.563009994032107 66-67 22.316234608734657 27.082546676546603 27.80922827667985 22.79199043803889 68-69 22.193685944233177 26.91403541569545 28.160612994905122 22.731665645166252 70-71 22.371323990569195 26.848021750871297 27.98536057304306 22.795293685516445 72-73 22.41267645546337 26.761716112910683 28.072000972666466 22.75360645895948 74-75 22.289456751104762 27.228091673842485 27.780921469733816 22.701530105318938 76-77 22.369931474893843 26.893596342551717 27.799133712440604 22.937338470113836 78-79 22.373971698492653 26.97229234638471 27.84078568504932 22.81295027007332 80-81 22.416887850111124 27.108265045188645 27.704389243726602 22.770457860973632 82-83 22.32945504876938 27.014083673940174 27.985756620391456 22.670704656898987 84-85 22.359278249915725 27.002584437781284 27.729538881890605 22.908598430412386 86-87 22.327863585095702 26.919958291326147 27.880644293485396 22.871533830092755 88-89 22.40771342169736 26.841714553323566 27.658250778495884 23.09232124648319 90-91 22.38026658538986 26.72434764006997 27.8592097092966 23.036176065243566 92-93 22.477117192158794 26.821277461735782 27.83339801414143 22.868207331963994 94-95 22.287947584989162 26.75491449867595 27.911830707236447 23.04530720909844 96-97 22.152466813279986 26.811004137220984 27.9707650262882 23.065764023210832 98-99 22.147391993533606 26.87366944783668 27.644984488278833 23.333954070350877 100-101 22.029108044786412 26.74170835430142 27.906129561712927 23.32305403919924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 158.5 2 149.0 3 200.0 4 190.5 5 205.5 6 338.5 7 735.5 8 1330.0 9 1694.0 10 1703.0 11 1657.0 12 1784.0 13 1976.5 14 2128.0 15 2287.5 16 2408.0 17 2518.0 18 2654.0 19 2876.5 20 3156.0 21 3643.5 22 4401.0 23 5313.5 24 6463.0 25 8132.0 26 10565.5 27 13364.0 28 16012.0 29 18986.0 30 22926.5 31 27626.0 32 31895.5 33 35660.0 34 40750.0 35 45336.5 36 49717.5 37 55117.0 38 58502.5 39 60145.5 40 62383.0 41 66160.5 42 69424.0 43 73360.5 44 78105.0 45 80874.5 46 81988.0 47 85703.0 48 94094.0 49 93220.5 50 84040.5 51 78216.0 52 74259.0 53 71944.5 54 68941.5 55 63522.5 56 57742.5 57 51670.5 58 46563.0 59 41682.5 60 35303.5 61 28366.5 62 21919.5 63 16796.5 64 12441.5 65 9112.5 66 6632.5 67 4912.5 68 3411.0 69 2104.0 70 1385.0 71 891.0 72 591.0 73 418.5 74 299.5 75 207.5 76 138.0 77 73.5 78 38.0 79 21.5 80 17.5 81 13.5 82 11.0 83 9.0 84 9.0 85 11.5 86 9.5 87 4.5 88 3.0 89 2.0 90 1.5 91 2.0 92 1.5 93 0.0 94 0.5 95 1.0 96 1.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03325647602458803 2 0.02691740378092545 3 0.02228136587138117 4 0.044846570798448726 5 0.09191654753310746 6 0.10913611691141477 7 0.05624743953518516 8 0.08188756756552187 9 0.06873635798538608 10-11 0.07051035208342597 12-13 0.067932147327608 14-15 0.062231712959239766 16-17 0.04931703592550928 18-19 0.05920409636525167 20-21 0.05523034958564228 22-23 0.034013380173085056 24-25 0.0084205586520294 26-27 0.002696471029020651 28-29 0.006670217808630032 30-31 0.004801610692028002 32-33 0.006102539697257263 34-35 0.006670217808630032 36-37 0.007261549174643332 38-39 0.005085449747714386 40-41 0.007947493559218761 42-43 0.004825263946668534 44-45 0.002388978718693735 46-47 0.0023653254640532027 48-49 5.440248567322366E-4 50-51 0.0028620438115043753 52-53 0.007072323137519077 54-55 0.012843717269808892 56-57 0.010336472277912496 58-59 0.005700434368368219 60-61 0.0031695361218312917 62-63 0.009579568129415472 64-65 0.0029566568300665036 66-67 0.02365325464053203 68-69 0.04584000749335107 70-71 0.057855860850741334 72-73 0.004588731400263214 74-75 0.013908113728632833 76-77 0.02308557652915926 78-79 0.026444338688114806 80-81 0.07391642075166259 82-83 0.028927930425370667 84-85 0.01237065217699825 86-87 0.030489045231645782 88-89 0.04662056489648862 90-91 0.04399505363138957 92-93 0.04418427966851383 94-95 0.013293129107979 96-97 0.01788186050824221 98-99 0.03758502162380539 100-101 0.07271010476499545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.52675472302764 #Duplication Level Percentage of deduplicated Percentage of total 1 78.11404196147537 45.71761374303558 2 12.710151597829208 14.877678501172959 3 3.9718591508774237 6.973800789534475 4 1.749733044664758 4.096247869434827 5 0.9289726548708268 2.7184877358012347 6 0.5469936680139473 1.9208258545740904 7 0.3629983103330026 1.487157915161318 8 0.2619204582913778 1.2263483535489967 9 0.17838230309862854 0.9396123570343986 >10 1.059456878027806 11.303235264406668 >50 0.07612688282191588 3.0428812736653916 >100 0.03725879693131828 3.661170711269062 >500 0.0011330807192144639 0.4464930392273992 >1k 8.093433708674716E-4 0.9616700278373259 >5k 1.6186867417349434E-4 0.6267765642963007 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7637 0.36127981137948617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5595 0.2646799194275534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4108 0.19433514012661113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3392 0.16046367948136928 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2863 0.1354385360716864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.7306509281064055E-5 0.0 10-11 0.0 0.0 0.0 7.095976392159609E-5 0.0 12-13 0.0 0.0 0.0 9.461301856212811E-5 0.0 14-15 0.0 0.0 0.0 1.655727824837242E-4 0.0 16-17 0.0 0.0 0.0 1.8922603712425622E-4 0.0 18-19 0.0 0.0 0.0 2.1287929176478824E-4 0.0 20-21 0.0 0.0 0.0 3.311455649674484E-4 0.0 22-23 0.0 0.0 0.0 5.913313660133007E-4 0.0 24-25 0.0 0.0 0.0 9.461301856212811E-4 0.0 26-27 0.0 0.0 0.0 0.0011353562227455374 0.0 28-29 0.0 0.0 0.0 0.0019395668805236262 0.0 30-31 0.0 0.0 0.0 0.003453375177517676 0.0 32-33 2.3653254640532027E-5 0.0 0.0 0.005440248567322366 0.0 34-35 4.7306509281064055E-5 0.0 0.0 0.007498081721048653 0.0 36-37 4.7306509281064055E-5 0.0 0.0 0.010170899495428772 0.0 38-39 4.7306509281064055E-5 0.0 0.0 0.012488918450200911 0.0 40-41 4.7306509281064055E-5 0.0 0.0 0.016509971739091356 0.0 42-43 4.7306509281064055E-5 0.0 0.0 0.021619074741446274 0.0 44-45 4.7306509281064055E-5 0.0 0.0 0.03238130560288835 0.0 46-47 4.7306509281064055E-5 0.0 0.0 0.052959637140151214 0.0 48-49 4.7306509281064055E-5 0.0 0.0 0.07043939231950438 0.0 50-51 4.7306509281064055E-5 0.0 0.0 0.08950391555977319 0.0 52-53 4.7306509281064055E-5 0.0 0.0 0.11214008025076234 0.0 54-55 4.7306509281064055E-5 0.0 0.0 0.1397197751616227 0.0 56-57 4.7306509281064055E-5 0.0 0.0 0.16770157540137207 0.0 58-59 4.7306509281064055E-5 0.0 0.0 0.20873997220269513 0.0 60-61 4.7306509281064055E-5 0.0 0.0 0.258482766711734 0.0 62-63 4.7306509281064055E-5 0.0 0.0 0.3130744784220819 0.0 64-65 4.7306509281064055E-5 0.0 0.0 0.3556030302657585 0.0 66-67 4.7306509281064055E-5 0.0 0.0 0.4265627941873546 0.0 68-69 4.7306509281064055E-5 0.0 0.0 0.5167999606409843 0.0 70-71 4.7306509281064055E-5 0.0 0.0 0.5805691351518586 0.0 72-73 4.7306509281064055E-5 0.0 0.0 0.6938918781346476 0.0 74-75 4.7306509281064055E-5 0.0 0.0 0.7853353605749444 0.0 76-77 4.7306509281064055E-5 0.0 0.0 0.8553963008202004 0.0 78-79 4.7306509281064055E-5 0.0 0.0 0.9474074613718699 0.0 80-81 4.7306509281064055E-5 0.0 0.0 1.0487616575065495 0.0 82-83 4.7306509281064055E-5 0.0 0.0 1.16970074848359 0.0 84-85 4.7306509281064055E-5 0.0 0.0 1.3589740921171272 0.0 86-87 4.7306509281064055E-5 0.0 0.0 1.6168891807174883 0.0 88-89 4.7306509281064055E-5 0.0 0.0 1.9284025443332953 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13580 0.0 26.883308 1 GTACATG 9235 0.0 24.141106 1 GGTATCA 9770 0.0 24.132835 1 TATCAAC 15740 0.0 22.955227 2 TCAACGC 15735 0.0 22.90209 4 ATCAACG 15725 0.0 22.886421 3 CAACGCA 15840 0.0 22.689714 5 GAGTACT 6955 0.0 22.615868 12-13 TACATGG 9365 0.0 22.539688 2 AACGCAG 16095 0.0 22.35606 6 ACATGGG 9390 0.0 21.264557 3 GTACTTT 7675 0.0 20.310183 14-15 ACGCAGA 17610 0.0 19.99757 7 AGTACTT 7585 0.0 19.954288 12-13 AGAGTAC 12315 0.0 19.910503 10-11 CGCAGAG 17600 0.0 19.900452 8 GCAGAGT 18305 0.0 18.613882 9 GTACCGT 180 1.2274948E-4 18.48485 6 GTATACG 155 9.5794967E-4 18.400974 1 CATGGGA 5525 0.0 17.381681 4 >>END_MODULE