FastQCFastQC Report
Thu 26 May 2016
SRR937032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113874
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.34216798162993634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.28421750776063287No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51320.24277700563042076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.16822194700346377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144550.025.1075631
GTACATG91900.022.3821431
GGTATCA108250.022.3367081
GAGTACT75800.022.3097512-13
TACATGG93400.021.3589732
TCAACGC168800.021.2166524
ATCAACG168600.021.2136483
CAACGCA170500.021.0051065
TATCAAC171500.020.8826272
AGAGTAC127000.020.75908910-11
ACATGGG91700.020.718993
AACGCAG174300.020.5758746
GTACTTT83250.020.19818514-15
AGTACTT81950.019.88195412-13
ACGCAGA191400.018.6631377
CGCAGAG192400.018.5171958
CATGGGA54550.017.9370254
GCAGAGT200900.017.4736469
GTGTAGC9850.017.3618341
CACCGTT2202.9181861E-517.273337
Produced by FastQC (version 0.11.5)