##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937032_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113874 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19228676827474 33.0 31.0 34.0 31.0 34.0 2 32.32399849754527 33.0 31.0 34.0 31.0 34.0 3 32.454195945453705 34.0 31.0 34.0 31.0 34.0 4 36.09986451415742 37.0 35.0 37.0 35.0 37.0 5 35.942716074846466 37.0 35.0 37.0 35.0 37.0 6 36.03488429300895 37.0 35.0 37.0 35.0 37.0 7 35.99341729923354 37.0 35.0 37.0 35.0 37.0 8 35.940291616245815 37.0 35.0 37.0 35.0 37.0 9 37.65102319248924 39.0 37.0 39.0 35.0 39.0 10-11 37.56891044593954 39.0 37.0 39.0 35.0 39.0 12-13 37.47002967064262 39.0 37.0 39.0 35.0 39.0 14-15 38.693951247803795 40.0 38.0 41.0 34.5 41.0 16-17 38.69461330240118 40.0 38.0 41.0 34.5 41.0 18-19 38.80541011432091 40.0 38.0 41.0 35.0 41.0 20-21 38.799864608770434 40.0 38.0 41.0 35.0 41.0 22-23 38.71128813732512 40.0 38.0 41.0 34.0 41.0 24-25 38.62062142776722 40.0 38.0 41.0 34.0 41.0 26-27 38.43391564492491 40.0 38.0 41.0 34.0 41.0 28-29 38.291557349208134 40.0 38.0 41.0 34.0 41.0 30-31 38.31123070722285 40.0 38.0 41.0 34.0 41.0 32-33 38.208497999407726 40.0 38.0 41.0 34.0 41.0 34-35 38.09573229057172 40.0 38.0 41.0 33.5 41.0 36-37 37.965152369535744 40.0 37.0 41.0 33.0 41.0 38-39 37.81569123798296 40.0 37.0 41.0 33.0 41.0 40-41 37.52117888767259 40.0 37.0 41.0 32.5 41.0 42-43 37.34407135903086 40.0 37.0 41.0 32.0 41.0 44-45 37.135512570758706 40.0 36.5 41.0 31.5 41.0 46-47 36.801367300037754 39.0 36.0 41.0 31.0 41.0 48-49 36.46389898357234 39.0 36.0 40.5 30.0 41.0 50-51 36.19364635735148 39.0 35.5 40.0 30.0 41.0 52-53 35.9452112566785 39.0 35.0 40.0 29.5 41.0 54-55 35.5631229675941 38.0 35.0 40.0 28.0 41.0 56-57 35.368347640398625 38.0 34.5 40.0 28.0 41.0 58-59 35.05134885049913 38.0 34.0 40.0 27.5 41.0 60-61 34.67515211408059 37.5 34.0 40.0 26.5 41.0 62-63 34.3070258681454 37.0 33.5 39.5 26.0 41.0 64-65 33.983901594891655 36.5 33.0 39.0 26.0 40.0 66-67 33.65793656575558 36.0 33.0 39.0 26.0 40.0 68-69 33.209822581667595 35.5 32.5 39.0 25.0 40.0 70-71 32.76123505942171 35.0 32.0 38.0 24.0 40.0 72-73 32.34581980761389 35.0 31.5 37.5 23.0 39.5 74-75 31.864857602676413 35.0 31.0 37.0 22.0 39.0 76-77 30.410295977905967 33.5 29.5 35.5 20.5 37.5 78-79 31.100637265986528 34.0 31.0 36.0 21.0 38.0 80-81 30.965962966572274 34.0 31.0 35.5 20.0 37.0 82-83 30.626219207010447 34.0 30.5 35.0 19.5 37.0 84-85 30.190877743895804 34.0 30.0 35.0 18.0 36.0 86-87 29.8231571512777 34.0 30.0 35.0 16.0 36.0 88-89 29.454890405009948 34.0 29.5 35.0 9.0 35.5 90-91 29.012209573512898 34.0 29.0 35.0 4.5 35.0 92-93 28.540023672177245 33.0 29.0 35.0 2.0 35.0 94-95 27.991872741705514 33.0 28.0 34.5 2.0 35.0 96-97 27.237421199182165 33.0 26.5 34.0 2.0 35.0 98-99 26.399386150735566 32.0 25.0 34.0 2.0 35.0 100-101 23.22134384547045 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09064741388259279 1101 2 0.19687454519474556 1101 3 0.09758013044723413 1101 4 -0.001649568696443282 1101 5 -0.07457553913669557 1101 6 -0.018998115734397913 1101 7 -0.03411687978378097 1101 8 0.050292921483830355 1101 9 0.03156278703648496 1101 10-11 0.06852752521130867 1101 12-13 0.0900082302189702 1101 14-15 0.14801403579087236 1101 16-17 0.14842449273790237 1101 18-19 -0.0516449091397817 1101 20-21 -0.03680672372689031 1101 22-23 -0.06847597741124645 1101 24-25 -0.003819852591909978 1101 26-27 0.053206104260389964 1101 28-29 0.007350558087857451 1101 30-31 0.061884259117157114 1101 32-33 0.07204037119705475 1101 34-35 -0.0307570554288219 1101 36-37 -0.07191272326425491 1101 38-39 0.037686423538623615 1101 40-41 0.07937968273132867 1101 42-43 0.15745779591370734 1101 44-45 -0.11285405656899883 1101 46-47 -0.1958731649982539 1101 48-49 -0.09861162020025915 1101 50-51 -0.11610407726194438 1101 52-53 -0.002652675349352762 1101 54-55 -0.19459316530279835 1101 56-57 0.019997808858377653 1101 58-59 -0.12730683922296038 1101 60-61 -0.067670572104781 1101 62-63 0.11524685282166303 1101 64-65 0.021836963459819003 1101 66-67 0.026330533844003412 1101 68-69 -0.1993736332613949 1101 70-71 -0.187847743040372 1101 72-73 -0.04180020535425655 1101 74-75 -0.22408742407953497 1101 76-77 -0.004967538407068872 1101 78-79 -0.08657768444514957 1101 80-81 -0.09864408746451048 1101 82-83 0.13688807038171547 1101 84-85 -0.10412144698922887 1101 86-87 -0.3968016826865828 1101 88-89 -0.12506174586814112 1101 90-91 -0.020971718281224128 1101 92-93 -0.2694512178780819 1101 94-95 -0.35604106056271334 1101 96-97 -0.49762645789092375 1101 98-99 -0.3964286276774871 1101 100-101 -0.0591534902103561 1102 1 -0.15777513965645085 1102 2 -0.04664470394350673 1102 3 0.05376186556912188 1102 4 0.021405098579329263 1102 5 -0.12751932140893985 1102 6 0.01803553737965302 1102 7 0.1211802671077038 1102 8 0.0815836727608712 1102 9 0.12020295758620136 1102 10-11 0.07283695112877098 1102 12-13 0.07808878450555312 1102 14-15 0.01857067573993021 1102 16-17 0.12217790165576758 1102 18-19 0.017888709365969646 1102 20-21 0.0649115785281893 1102 22-23 -0.13846331077227347 1102 24-25 -0.051272681474621606 1102 26-27 0.09532925444657536 1102 28-29 0.06319832308363971 1102 30-31 -0.014109691351443132 1102 32-33 -0.09127690388357479 1102 34-35 0.12080516337871217 1102 36-37 0.06043046651382866 1102 38-39 0.20562944442820452 1102 40-41 0.29841805641794394 1102 42-43 0.16041680583936824 1102 44-45 0.1829220000225007 1102 46-47 0.015466937719196494 1102 48-49 0.159503334739739 1102 50-51 0.1328947045257891 1102 52-53 0.034719793053263004 1102 54-55 0.2655726776862579 1102 56-57 0.2988064526281917 1102 58-59 0.1766738078138772 1102 60-61 0.14888742448976444 1102 62-63 0.03935741942880355 1102 64-65 0.03857526931416544 1102 66-67 0.15777459990433584 1102 68-69 0.1529274728199823 1102 70-71 0.03231263183677413 1102 72-73 -0.03628606440155835 1102 74-75 0.04141674968183651 1102 76-77 0.13617688896394498 1102 78-79 -0.19407834200291063 1102 80-81 0.09894613638199345 1102 82-83 0.193097838229221 1102 84-85 0.23518839422787963 1102 86-87 0.04424549535237432 1102 88-89 -0.04783781630295891 1102 90-91 -0.15388594506251252 1102 92-93 -0.32402747381340546 1102 94-95 -0.2639109887158746 1102 96-97 -0.04926239238432828 1102 98-99 -0.12305833267631172 1102 100-101 0.0519436622184557 1103 1 0.03970511064632376 1103 2 0.21598321901879558 1103 3 0.15017532624548835 1103 4 0.13144303962981496 1103 5 -0.02747968681569546 1103 6 0.025139522328473163 1103 7 -0.004963407937019326 1103 8 0.1078236235769765 1103 9 0.20178368763121313 1103 10-11 0.09097867219517042 1103 12-13 0.2171110339572948 1103 14-15 0.2420918144268498 1103 16-17 0.12031180253939766 1103 18-19 0.05311101887442504 1103 20-21 0.12943449045400257 1103 22-23 0.23123244925129427 1103 24-25 0.17782647474260926 1103 26-27 0.0934161915392977 1103 28-29 -0.020327668528345555 1103 30-31 0.1750127830995396 1103 32-33 0.09606717277271315 1103 34-35 0.20033122757109822 1103 36-37 0.19034494260428403 1103 38-39 0.14167383374948628 1103 40-41 0.36521230804993365 1103 42-43 0.03219711776433343 1103 44-45 0.06309210856578318 1103 46-47 0.12127173343381514 1103 48-49 0.06216500758996801 1103 50-51 0.017222175924125338 1103 52-53 -0.0946875096739106 1103 54-55 0.05983836323663638 1103 56-57 -0.0030152443585862443 1103 58-59 -0.12088450200197798 1103 60-61 -0.08446017587399268 1103 62-63 0.20100803301784964 1103 64-65 0.12842536231670465 1103 66-67 -0.0035702153643057954 1103 68-69 0.03048077316480402 1103 70-71 -0.03488952878223728 1103 72-73 0.19221732766497723 1103 74-75 -0.014542741913778912 1103 76-77 0.026620860449806827 1103 78-79 0.09845359693277445 1103 80-81 0.1043720552712557 1103 82-83 0.2968034948043652 1103 84-85 -0.0016768652328345013 1103 86-87 -0.19491707621403975 1103 88-89 -0.07644623648372928 1103 90-91 -0.37413847505885656 1103 92-93 -0.3742283863340923 1103 94-95 -0.21999023797833317 1103 96-97 -0.007041766456616472 1103 98-99 -0.0968939864478564 1103 100-101 0.4843469345984843 1104 1 0.02423622958544769 1104 2 0.15355062865942415 1104 3 0.08073503363979029 1104 4 0.017142585894532658 1104 5 -0.12367423495405916 1104 6 0.06795723660556519 1104 7 0.08285310558319026 1104 8 0.09538389828539806 1104 9 0.052514911834762756 1104 10-11 0.1030756693748387 1104 12-13 0.18476968178515563 1104 14-15 0.08492095619698858 1104 16-17 0.10446625893297323 1104 18-19 0.16095068515296163 1104 20-21 0.11888764914069583 1104 22-23 0.06099838093615517 1104 24-25 0.11778389331252725 1104 26-27 0.07876067341366166 1104 28-29 0.005185321495510209 1104 30-31 0.09047292435186449 1104 32-33 0.11810647561443233 1104 34-35 0.15037800101553955 1104 36-37 0.14377094755658248 1104 38-39 0.0470129093175089 1104 40-41 0.3287352088619997 1104 42-43 0.14674352363407905 1104 44-45 0.12254291601194467 1104 46-47 -0.024429479581996816 1104 48-49 0.04421678592831313 1104 50-51 0.22126402800557088 1104 52-53 0.10740132390582602 1104 54-55 0.1917483710521921 1104 56-57 0.14366494323019197 1104 58-59 -0.04449519148392511 1104 60-61 0.0706477394921805 1104 62-63 0.18808133410154682 1104 64-65 0.08160667196541027 1104 66-67 -0.08994137337733576 1104 68-69 -0.010674637260080999 1104 70-71 0.19888961857596854 1104 72-73 0.02210671881356774 1104 74-75 -0.08364243335213217 1104 76-77 0.25675007426535856 1104 78-79 0.4236752148879006 1104 80-81 0.19496703514069225 1104 82-83 0.22990625445692103 1104 84-85 0.2107843035270669 1104 86-87 0.20294665934255463 1104 88-89 0.2816590078267929 1104 90-91 0.18486983123972323 1104 92-93 0.051417209015088616 1104 94-95 0.17259848171319092 1104 96-97 0.4232333170839695 1104 98-99 0.5283159526480539 1104 100-101 1.125393619484683 1105 1 -0.04699747886085959 1105 2 0.05940015898044493 1105 3 0.049071211934986536 1105 4 0.05431400112806273 1105 5 0.042811212014505884 1105 6 0.07303798174542919 1105 7 0.09469721138995624 1105 8 0.11432284897129108 1105 9 0.13882802865671096 1105 10-11 0.12496726570869043 1105 12-13 0.05908000930904933 1105 14-15 0.08402853073616967 1105 16-17 -0.06788647941395709 1105 18-19 -0.02655871071706173 1105 20-21 0.0882843895181864 1105 22-23 0.11951919094031638 1105 24-25 0.019478106622621283 1105 26-27 0.06715966296982856 1105 28-29 0.07054080708032018 1105 30-31 0.06528928584622662 1105 32-33 0.18082372582795614 1105 34-35 0.0665061446498143 1105 36-37 0.029489891421228265 1105 38-39 -0.007012562495717134 1105 40-41 0.06705538763027619 1105 42-43 0.09592232979951376 1105 44-45 0.12088185112307315 1105 46-47 -0.1751709881100112 1105 48-49 -0.054144386942788 1105 50-51 -0.13375150302922378 1105 52-53 -0.09871231104759914 1105 54-55 0.08881533960824584 1105 56-57 -0.08761827546485534 1105 58-59 -0.15160987960766903 1105 60-61 -0.16238014391033317 1105 62-63 -0.06586888746399922 1105 64-65 -0.003948841020985583 1105 66-67 0.036121427056258426 1105 68-69 0.011285671253439489 1105 70-71 -0.2381271317876923 1105 72-73 -0.0660988980115107 1105 74-75 -0.040066834754526326 1105 76-77 0.06668697982691896 1105 78-79 0.1606560266993995 1105 80-81 0.1289239072123607 1105 82-83 0.15793919965666703 1105 84-85 -0.17458941368941439 1105 86-87 -0.07748794311167728 1105 88-89 -0.17882555462862015 1105 90-91 -0.1319393333825829 1105 92-93 -0.14238944990306734 1105 94-95 -0.12831995026654042 1105 96-97 0.01865975111756768 1105 98-99 0.14547344637754378 1105 100-101 0.38279527465698493 1106 1 0.009462997849688293 1106 2 0.07278673625845045 1106 3 0.09632868828670382 1106 4 0.10802409097721721 1106 5 -7.231849813109648E-4 1106 6 0.09936931929970427 1106 7 0.1352618335343294 1106 8 0.17815389328418973 1106 9 0.2393802781873049 1106 10-11 0.24592759427804367 1106 12-13 0.22532723220464135 1106 14-15 0.2725870777881525 1106 16-17 0.3011795098868504 1106 18-19 0.1783902913499631 1106 20-21 0.11583213573015172 1106 22-23 0.20378568348677817 1106 24-25 0.2583529633687789 1106 26-27 0.3853417576081668 1106 28-29 0.2700737495989287 1106 30-31 0.2708156475939987 1106 32-33 0.2497688996831684 1106 34-35 0.26218871933513554 1106 36-37 0.2713944745883978 1106 38-39 0.28408141463058456 1106 40-41 0.3989277272793714 1106 42-43 0.40990260138881496 1106 44-45 0.07968143823921991 1106 46-47 0.030007828306978013 1106 48-49 -0.10115119622467716 1106 50-51 0.06490041207587893 1106 52-53 0.14187594417926874 1106 54-55 0.2556542584294803 1106 56-57 0.054774087574053 1106 58-59 0.15623447916684796 1106 60-61 0.017237365075878586 1106 62-63 0.13436892042857806 1106 64-65 0.08988535726579272 1106 66-67 0.17062016677000003 1106 68-69 0.12327064165561552 1106 70-71 0.09595885440259622 1106 72-73 0.05319147524348722 1106 74-75 0.03141064126701565 1106 76-77 0.0751696924877372 1106 78-79 0.011201976970877325 1106 80-81 0.013097257610169777 1106 82-83 0.26786626726900664 1106 84-85 0.08100997602098303 1106 86-87 0.0983652155106185 1106 88-89 0.1692161143188109 1106 90-91 0.06102109249946608 1106 92-93 0.2930781158633735 1106 94-95 0.6160932585413015 1106 96-97 0.6296694224352564 1106 98-99 0.42841401004394086 1106 100-101 0.817674619951088 1107 1 0.23871687114357343 1107 2 0.25799457706556694 1107 3 0.23676925371496793 1107 4 0.1552323344471418 1107 5 -0.06255995332734443 1107 6 0.029834777884524044 1107 7 0.08102684381810832 1107 8 0.12980189125126174 1107 9 0.16934170757487266 1107 10-11 0.2226805905424385 1107 12-13 0.285840631953981 1107 14-15 0.3032978243539617 1107 16-17 0.3151201913959909 1107 18-19 0.25937806712376954 1107 20-21 0.2613376340393927 1107 22-23 0.1857555454369546 1107 24-25 0.19934639604117876 1107 26-27 0.2608020521125596 1107 28-29 0.3417324307427734 1107 30-31 0.23696779807963964 1107 32-33 0.22124045006108872 1107 34-35 0.26183907535538964 1107 36-37 0.2087570518919435 1107 38-39 0.26031846997897645 1107 40-41 0.3344695633376915 1107 42-43 0.275657297525548 1107 44-45 0.19645972471745665 1107 46-47 0.18236595156197666 1107 48-49 0.21034503710300356 1107 50-51 -0.09986855429990982 1107 52-53 0.169621840592157 1107 54-55 0.4323286373501247 1107 56-57 0.2151017345798394 1107 58-59 0.10096632302150255 1107 60-61 0.07521205057147284 1107 62-63 -0.011385205814150368 1107 64-65 0.09812978480026402 1107 66-67 0.09994899046675698 1107 68-69 -0.006298728690929067 1107 70-71 -0.1885697148037906 1107 72-73 0.11305755482591451 1107 74-75 0.16505078310522237 1107 76-77 -0.0963277600393937 1107 78-79 0.21295075742108338 1107 80-81 0.29840908071507144 1107 82-83 0.40519682306980087 1107 84-85 0.24926218587837567 1107 86-87 0.44596294882732224 1107 88-89 0.4673426111664085 1107 90-91 0.4581850056905985 1107 92-93 0.4990332511921771 1107 94-95 0.6584473598394922 1107 96-97 0.532621963812133 1107 98-99 0.5163511058921664 1107 100-101 0.9625139962628353 1108 1 0.01043330106837459 1108 2 0.11296274934829853 1108 3 0.09010189527855772 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-1.1001223559206963 2316 52-53 -1.1754293771207287 2316 54-55 -1.197141191259007 2316 56-57 -1.1612914179943417 2316 58-59 -1.0805460464507846 2316 60-61 -1.0546259814212817 2316 62-63 -1.0304325535162562 2316 64-65 -1.1216429879731606 2316 66-67 -1.1007796003021966 2316 68-69 -1.0041093758708755 2316 70-71 -1.0209650191790658 2316 72-73 -1.2352156881081946 2316 74-75 -0.8803720656776974 2316 76-77 -0.887612441621517 2316 78-79 -1.0675382402513627 2316 80-81 -1.0219867013530646 2316 82-83 -1.0791193283850333 2316 84-85 -0.9737410175893082 2316 86-87 -0.9270342834796459 2316 88-89 -1.4511950373839504 2316 90-91 -1.519725629503359 2316 92-93 -1.420295821767109 2316 94-95 -1.4539507347161837 2316 96-97 -1.322730543169321 2316 98-99 -1.728430678203324 2316 100-101 -2.352074040587631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 46.0 6 86.0 7 214.0 8 505.0 9 991.0 10 1619.0 11 2624.0 12 4100.0 13 5678.0 14 7288.0 15 10081.0 16 13500.0 17 18313.0 18 16686.0 19 10685.0 20 9674.0 21 10071.0 22 10591.0 23 11843.0 24 13651.0 25 16600.0 26 20277.0 27 25488.0 28 32238.0 29 40421.0 30 50832.0 31 64292.0 32 82844.0 33 109264.0 34 151070.0 35 217298.0 36 338538.0 37 493255.0 38 308035.0 39 15160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94475015259121 19.496046823027317 15.119399665955363 22.43980335842611 2 15.53826815787818 21.04023688066129 39.594532518815576 23.826962442644952 3 19.72378610027395 24.927987495913893 29.826919265101104 25.52130713871106 4 13.243883031817413 17.43892966184361 37.155431213024045 32.16175609331493 5 13.899232151701959 37.658769938344115 33.81640615205866 14.62559175789527 6 31.511764655793108 36.54602876046538 17.476065271629246 14.466141312112264 7 28.26777427183318 33.00529360840543 20.700658034789274 18.026274084972112 8 26.350435267191898 34.758173855206124 19.71205473930802 19.179336138293955 9 26.356089678088363 17.07179999545857 20.317928427076 36.254181899377066 10-11 24.733646549805368 26.994496122875848 27.287205218287657 20.98465210903112 12-13 26.062490405078943 24.304901931331944 27.7380208667209 21.894586796868218 14-15 22.854208415885612 25.61133629669999 26.35213065305713 25.18232463435727 16-17 22.042349733238595 28.99903210882011 26.729265793514656 22.229352364426642 18-19 21.961283168571633 27.642333152463667 29.02106619120179 21.375317487762914 20-21 22.92774436858518 26.50121389536468 29.046656915135184 21.524384820914953 22-23 22.590826894624364 26.61061550233757 28.932571844874914 21.865985758163156 24-25 22.524266057606518 26.739189671207807 29.017890511093935 21.718653760091737 26-27 22.40855266674772 26.745320306339043 29.078650643583536 21.767476383329697 28-29 22.009292895079316 27.106689947787782 29.143874773933913 21.74014238319899 30-31 22.32318482558563 26.620910234006377 29.06398394606301 21.99192099434498 32-33 22.254440674630583 26.861563730564065 28.995992181157952 21.8880034136474 34-35 22.585286540257364 26.81299831494214 28.825842978342138 21.77587216645836 36-37 22.55164353618755 26.568548304118355 28.919741617335077 21.96006654235902 38-39 22.282845376734727 26.814269131953967 29.0306479812253 21.87223751008601 40-41 22.50169003703653 26.779789948146966 28.79510885068493 21.923411164131572 42-43 22.345986563059103 26.985808993345866 28.4586971598118 22.209507283783232 44-45 22.45674372177846 26.90570072214036 28.411811595402714 22.225743960678475 46-47 22.75190657509997 27.19463694297045 28.017724844822457 22.035731637107126 48-49 22.788274586422062 27.360315440400782 27.729590750613802 22.121819222563353 50-51 22.52154313440394 27.432746226595622 27.87417026024433 22.17154037875611 52-53 22.868078801514567 27.632212607409734 27.37626001245111 22.12344857862459 54-55 22.576700408822852 27.30834477362416 27.578914353457208 22.536040464095777 56-57 22.676157947272067 27.60675916683618 27.45878412579771 22.258298760094046 58-59 23.070742287841732 27.261420995614678 27.398657438726126 22.269179277817464 60-61 22.7337312677329 27.280803942601885 27.985566500656983 21.999898289008236 62-63 22.585320877838925 27.376337178250537 27.870075449187343 22.168266494723195 64-65 22.508891851853953 27.56004557510182 27.619935630017594 22.31112694302663 66-67 22.51767786373114 27.60734150238484 27.404751232631106 22.470229401252915 68-69 22.45186915202569 27.435470342825703 27.62647046440349 22.486190040745115 70-71 22.602767915642673 27.421760613851877 27.54769107771783 22.427780392787618 72-73 22.6658116773221 27.2907726197468 27.545211582661437 22.49820412026966 74-75 22.563541055359504 27.771505534673047 27.24965175449739 22.415301655470063 76-77 22.56789375896607 27.551425168736525 27.28733639327821 22.593344679019193 78-79 22.61407873430189 27.524458705813842 27.227018325165847 22.634444234718423 80-81 22.68167601261875 27.704174183600117 27.14438685831486 22.469762945466268 82-83 22.60680873770976 27.655908210147068 27.2950774692129 22.44220558293028 84-85 22.55153803977336 27.653303699877046 27.16790446900338 22.627253791346217 86-87 22.589011568127347 27.55696487269667 27.176690508420936 22.67733305075505 88-89 22.624451085883106 27.54078196829692 26.913934760677584 22.92083218514239 90-91 22.60000141921475 27.38734391594464 27.138058845374186 22.87459581946642 92-93 22.650645840444096 27.460843801674983 27.125700757401045 22.762809600479876 94-95 22.49431612290988 27.431625536810618 27.19700890403118 22.877049436248328 96-97 22.32039923003743 27.437603749333565 27.286435441363682 22.955561579265318 98-99 22.24059829898818 27.60786608570721 26.843576879163443 23.307958736141167 100-101 22.04105373184672 27.67106963418935 26.92671321545463 23.361163418509303 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 198.5 2 159.5 3 193.5 4 197.5 5 222.0 6 340.0 7 696.0 8 1226.0 9 1638.5 10 1672.5 11 1598.0 12 1736.0 13 1967.5 14 2193.5 15 2363.5 16 2576.5 17 2738.0 18 2911.5 19 3174.0 20 3523.5 21 4000.0 22 4694.0 23 5724.5 24 6780.5 25 8403.5 26 10818.0 27 13524.5 28 16139.5 29 19088.0 30 23028.5 31 27420.5 32 31599.5 33 35421.5 34 40681.5 35 45477.0 36 49543.5 37 54628.5 38 58052.0 39 60084.0 40 62137.5 41 65608.0 42 69091.5 43 72794.0 44 77587.0 45 80645.0 46 81808.0 47 85995.5 48 94960.0 49 93861.5 50 84139.5 51 77986.5 52 74027.0 53 71557.5 54 68551.5 55 63303.5 56 57075.5 57 51249.0 58 46758.0 59 42004.0 60 35516.5 61 28391.5 62 21919.0 63 16890.0 64 12543.0 65 9255.5 66 6850.0 67 5073.5 68 3453.0 69 2092.0 70 1405.5 71 932.0 72 612.0 73 415.5 74 306.0 75 227.0 76 136.5 77 59.0 78 35.5 79 19.5 80 8.5 81 7.0 82 7.0 83 4.0 84 2.0 85 1.5 86 1.5 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0181656995639286 2 0.0031695361218312917 3 2.3653254640532026E-4 4 0.0 5 0.0021287929176478826 6 0.0 7 1.8922603712425622E-4 8 0.0 9 9.461301856212811E-5 10-11 2.3653254640532027E-5 12-13 0.005936966914773539 14-15 0.002933003575425971 16-17 0.0 18-19 6.149846206538328E-4 20-21 8.515171670591531E-4 22-23 0.0014901550423535178 24-25 7.569041484970249E-4 26-27 3.311455649674484E-4 28-29 4.7306509281064055E-5 30-31 0.0 32-33 2.8383905568638435E-4 34-35 0.0 36-37 0.0015374615516345817 38-39 0.008444211906669935 40-41 7.095976392159608E-4 42-43 0.0 44-45 8.988236763402171E-4 46-47 2.3653254640532026E-4 48-49 1.8922603712425622E-4 50-51 0.0015847680609156459 52-53 2.8383905568638435E-4 54-55 0.0 56-57 1.8922603712425622E-4 58-59 1.8922603712425622E-4 60-61 0.0019632201351641584 62-63 4.7306509281064055E-5 64-65 2.3653254640532027E-5 66-67 7.095976392159609E-5 68-69 4.7306509281064055E-5 70-71 4.2575858352957653E-4 72-73 3.547988196079804E-4 74-75 0.0030039633393475675 76-77 7.095976392159609E-5 78-79 2.3653254640532026E-4 80-81 0.001679381079477774 82-83 9.461301856212811E-5 84-85 0.0022234059362100105 86-87 2.601858010458523E-4 88-89 0.0019632201351641584 90-91 0.0013718887691508577 92-93 2.3653254640532027E-5 94-95 0.0 96-97 2.3653254640532026E-4 98-99 6.859443845754289E-4 100-101 0.00163207457019671 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.9206010719804 #Duplication Level Percentage of deduplicated Percentage of total 1 77.07321183086813 43.870535439610855 2 13.19836295564344 15.02517505202769 3 4.174415318328016 7.128306871299395 4 1.8522321996391373 4.217206805133444 5 0.9589858404869416 2.729302523001752 6 0.6054992503626461 2.067922876756522 7 0.4050378658575185 1.6138499147065488 8 0.2849630008431213 1.297621223301258 9 0.20072786119123112 1.0282995459808133 >10 1.1219137128270804 11.668250718593045 >50 0.08133048264665278 3.1549310729392035 >100 0.04115511565011484 4.113627870565231 >500 0.0010822828279614554 0.41729729937856075 >1k 8.325252522780107E-4 0.7970160434076551 >5k 2.497575756834032E-4 0.8706567432979873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7233 0.34216798162993634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6008 0.28421750776063287 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5132 0.24277700563042076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3556 0.16822194700346377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.7306509281064055E-5 0.0 2 0.0 0.0 0.0 4.7306509281064055E-5 0.0 3 0.0 0.0 0.0 4.7306509281064055E-5 0.0 4 0.0 0.0 0.0 4.7306509281064055E-5 0.0 5 0.0 0.0 0.0 4.7306509281064055E-5 0.0 6 0.0 0.0 0.0 9.461301856212811E-5 0.0 7 0.0 0.0 0.0 9.461301856212811E-5 0.0 8 0.0 0.0 0.0 9.461301856212811E-5 0.0 9 0.0 0.0 0.0 9.461301856212811E-5 0.0 10-11 0.0 0.0 0.0 1.1826627320266014E-4 0.0 12-13 0.0 0.0 0.0 1.4191952784319218E-4 0.0 14-15 0.0 0.0 0.0 2.1287929176478824E-4 0.0 16-17 0.0 0.0 0.0 2.3653254640532029E-4 0.0 18-19 0.0 0.0 0.0 2.3653254640532029E-4 0.0 20-21 0.0 0.0 0.0 2.8383905568638435E-4 0.0 22-23 0.0 0.0 0.0 5.440248567322366E-4 0.0 24-25 0.0 0.0 0.0 9.934366949023452E-4 0.0 26-27 0.0 0.0 0.0 0.0011590094773860694 0.0 28-29 0.0 0.0 0.0 0.0020105266444452223 0.0 30-31 0.0 0.0 0.0 0.0035716414507203365 0.0 32-33 0.0 0.0 0.0 0.005629474604446623 0.0 34-35 0.0 0.0 0.0 0.007640001248891845 0.0 36-37 0.0 0.0 0.0 0.010194552750069304 0.0 38-39 0.0 0.0 0.0 0.012441611940919847 0.0 40-41 0.0 0.0 0.0 0.016391705465888695 0.0 42-43 0.0 0.0 0.0 0.021524461722884147 0.0 44-45 0.0 0.0 0.0 0.032499571876091 0.0 46-47 0.0 0.0 0.0 0.05298329039479174 0.0 48-49 0.0 0.0 0.0 0.07051035208342597 0.0 50-51 0.0 0.0 0.0 0.08938564928657053 0.0 52-53 0.0 0.0 0.0 0.1116670151579517 0.0 54-55 0.0 0.0 0.0 0.13922305681417152 0.0 56-57 0.0 0.0 0.0 0.16737042983640463 0.0 58-59 0.0 0.0 0.0 0.20845613314700878 0.0 60-61 0.0 0.0 0.0 0.25826988741996926 0.0 62-63 0.0 0.0 0.0 0.31350023700561147 0.0 64-65 0.0 0.0 0.0 0.3560051355946475 0.0 66-67 0.0 0.0 0.0 0.4268702864976815 0.0 68-69 0.0 0.0 0.0 0.5167763073863438 0.0 70-71 0.0 0.0 0.0 0.5805927884064992 0.0 72-73 0.0 0.0 0.0 0.6942466769542555 0.0 74-75 0.0 0.0 0.0 0.7858557321770361 0.0 76-77 0.0 0.0 0.0 0.8559403256769325 0.0 78-79 0.0 0.0 0.0 0.9481170590110858 0.0 80-81 0.0 0.0 0.0 1.0490218433075955 0.0 82-83 0.0 0.0 0.0 1.1699136277753546 0.0 84-85 0.0 0.0 0.0 1.3591633181542515 0.0 86-87 0.0 0.0 0.0 1.6174332055742204 0.0 88-89 0.0 0.0 0.0 1.9287336898982628 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14455 0.0 25.107563 1 GTACATG 9190 0.0 22.382143 1 GGTATCA 10825 0.0 22.336708 1 GAGTACT 7580 0.0 22.30975 12-13 TACATGG 9340 0.0 21.358973 2 TCAACGC 16880 0.0 21.216652 4 ATCAACG 16860 0.0 21.213648 3 CAACGCA 17050 0.0 21.005106 5 TATCAAC 17150 0.0 20.882627 2 AGAGTAC 12700 0.0 20.759089 10-11 ACATGGG 9170 0.0 20.71899 3 AACGCAG 17430 0.0 20.575874 6 GTACTTT 8325 0.0 20.198185 14-15 AGTACTT 8195 0.0 19.881954 12-13 ACGCAGA 19140 0.0 18.663137 7 CGCAGAG 19240 0.0 18.517195 8 CATGGGA 5455 0.0 17.937025 4 GCAGAGT 20090 0.0 17.473646 9 GTGTAGC 985 0.0 17.361834 1 CACCGTT 220 2.9181861E-5 17.27333 7 >>END_MODULE