##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937031_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356961 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78542473827673 33.0 31.0 34.0 30.0 34.0 2 31.797829454758364 33.0 31.0 34.0 30.0 34.0 3 31.69634217743675 33.0 31.0 34.0 30.0 34.0 4 35.36844921433994 37.0 35.0 37.0 33.0 37.0 5 35.427343603362836 37.0 35.0 37.0 33.0 37.0 6 35.58122315883248 37.0 35.0 37.0 33.0 37.0 7 35.552449707391006 37.0 35.0 37.0 33.0 37.0 8 35.52695392493858 37.0 35.0 37.0 33.0 37.0 9 37.07625202753242 39.0 37.0 39.0 33.0 39.0 10-11 36.94765534610224 39.0 37.0 39.0 33.0 39.0 12-13 36.83345939752522 39.0 37.0 39.0 32.5 39.0 14-15 37.65184712055378 40.0 37.0 41.0 32.0 41.0 16-17 37.66275027243873 40.0 37.0 41.0 32.5 41.0 18-19 37.81604292905948 40.0 37.0 41.0 33.0 41.0 20-21 37.84411882530584 40.0 37.5 41.0 33.0 41.0 22-23 37.70460358414505 40.0 37.0 41.0 32.5 41.0 24-25 37.443308932908636 40.0 36.5 41.0 32.0 41.0 26-27 37.285954207882654 39.0 36.0 41.0 32.0 41.0 28-29 37.243386532422306 39.0 36.0 41.0 32.0 41.0 30-31 36.87150416992333 39.0 35.5 41.0 32.0 41.0 32-33 36.34284837839428 38.0 35.0 40.5 31.0 41.0 34-35 35.9416112684579 38.0 35.0 40.0 30.0 41.0 36-37 35.56448883771617 38.0 35.0 40.0 30.0 41.0 38-39 35.17484963343334 38.0 35.0 40.0 28.5 41.0 40-41 34.77221881382 38.0 35.0 40.0 26.0 41.0 42-43 34.1563336050717 38.0 34.5 40.0 23.0 41.0 44-45 33.30582892809018 37.5 33.0 40.0 13.0 41.0 46-47 32.11434862632052 37.0 32.5 40.0 2.0 41.0 48-49 31.087873745311114 36.5 31.0 40.0 2.0 41.0 50-51 30.119606903835432 35.5 29.0 39.5 2.0 40.5 52-53 30.217347273231532 36.0 30.0 39.0 2.0 40.5 54-55 30.357597888845 36.0 30.0 40.0 2.0 41.0 56-57 30.09633405329994 35.5 29.5 40.0 2.0 41.0 58-59 29.822830785435944 35.0 28.5 39.5 2.0 41.0 60-61 29.42868408593656 35.0 28.0 39.0 2.0 41.0 62-63 29.115161040001567 35.0 27.0 39.0 2.0 41.0 64-65 28.793641322161243 35.0 26.5 38.5 2.0 40.5 66-67 28.3641014004331 35.0 26.0 38.0 2.0 40.0 68-69 27.90546586321755 35.0 25.0 37.0 2.0 39.5 70-71 27.51516972442368 34.5 24.5 36.5 2.0 39.0 72-73 27.19041015685187 34.0 23.5 36.0 2.0 39.0 74-75 26.837517263790723 34.0 22.5 35.5 2.0 38.5 76-77 26.48215071114211 34.0 20.5 35.0 2.0 37.0 78-79 26.074110336983594 34.0 19.0 35.0 2.0 37.0 80-81 25.643574508139544 33.5 16.0 35.0 2.0 36.0 82-83 25.259781880933772 33.0 9.5 35.0 2.0 36.0 84-85 24.921936850244144 33.0 6.0 35.0 2.0 35.5 86-87 24.67353296298475 33.0 2.0 35.0 2.0 35.0 88-89 24.25016318309283 33.0 2.0 35.0 2.0 35.0 90-91 23.84194211692594 32.0 2.0 35.0 2.0 35.0 92-93 23.482062466207793 32.0 2.0 35.0 2.0 35.0 94-95 23.03698163104653 32.0 2.0 35.0 2.0 35.0 96-97 22.599538885200346 31.5 2.0 35.0 2.0 35.0 98-99 22.08893828737593 31.0 2.0 35.0 2.0 35.0 100-101 20.336514353108605 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4299610878760163 1101 2 0.46263258081651415 1101 3 0.44928781055313394 1101 4 0.5001776903482025 1101 5 0.38327096672943384 1101 6 0.4396455697808719 1101 7 0.31094519945762045 1101 8 0.3634167920283531 1101 9 0.15510106794674527 1101 10-11 0.17012401899744845 1101 12-13 0.31132931827717414 1101 14-15 0.738645293697239 1101 16-17 0.7450062242200488 1101 18-19 0.6946032957693831 1101 20-21 0.6921775879802041 1101 22-23 0.9542688774041252 1101 24-25 1.0975499636455552 1101 26-27 0.9705395875651206 1101 28-29 0.8562990193552054 1101 30-31 0.924772613867475 1101 32-33 1.2308987978348895 1101 34-35 1.1382447466073344 1101 36-37 1.4554300757947942 1101 38-39 1.8419205705531994 1101 40-41 1.429338814369551 1101 42-43 1.3982783287580887 1101 44-45 1.4809826289958323 1101 46-47 1.928828113902128 1101 48-49 1.8884754829457968 1101 50-51 1.8072524237252985 1101 52-53 2.147533540863744 1101 54-55 2.068548630854526 1101 56-57 1.9061955174092766 1101 58-59 1.872261257363217 1101 60-61 2.1571576798911174 1101 62-63 2.4279477513157026 1101 64-65 2.456995452191215 1101 66-67 2.4593954078016615 1101 68-69 2.359398797843326 1101 70-71 2.440848048622186 1101 72-73 2.348203863289992 1101 74-75 2.430802528884186 1101 76-77 2.4002562433467887 1101 78-79 2.4874209045878857 1101 80-81 2.3857082497784603 1101 82-83 2.3367569461662185 1101 84-85 2.260740710770971 1101 86-87 2.1345573671682097 1101 88-89 2.5807066551012703 1101 90-91 2.7529397272777096 1101 92-93 2.3805129434509134 1101 94-95 2.1343315327982566 1101 96-97 2.4397781162267442 1101 98-99 2.5467010880425285 1101 100-101 2.6581730092094453 1102 1 0.2962132567597706 1102 2 0.2599522971113011 1102 3 0.2289596622471457 1102 4 -0.021465486419629087 1102 5 0.19078824169823605 1102 6 0.038234653827018406 1102 7 0.010703197692066624 1102 8 0.022011659764494595 1102 9 0.041040613166067885 1102 10-11 0.14647177096846775 1102 12-13 0.0038348736636137915 1102 14-15 0.2309324386852083 1102 16-17 0.24155123046033822 1102 18-19 0.1674846388030744 1102 20-21 0.09863401244216874 1102 22-23 0.4377130647956804 1102 24-25 0.33626796309763307 1102 26-27 0.41596742842232715 1102 28-29 0.3559755893226253 1102 30-31 0.6060570496533018 1102 32-33 0.6415788380163221 1102 34-35 0.21669745733152013 1102 36-37 0.4820426598866092 1102 38-39 0.5347267256169772 1102 40-41 0.5960765088080748 1102 42-43 0.7274007299026479 1102 44-45 0.5861811008840618 1102 46-47 0.8836468408815108 1102 48-49 1.423090106549342 1102 50-51 1.340036813850407 1102 52-53 1.5275866594902716 1102 54-55 1.590401085878728 1102 56-57 1.7543774601203062 1102 58-59 1.6817495530010973 1102 60-61 1.4961465995587098 1102 62-63 1.492413064395972 1102 64-65 1.765749371402201 1102 66-67 1.5331945311160204 1102 68-69 1.6031242552114477 1102 70-71 1.5920458812604643 1102 72-73 1.8766466808602331 1102 74-75 1.898653583646599 1102 76-77 1.7555155961823203 1102 78-79 1.7139018336311445 1102 80-81 1.700626364904334 1102 82-83 1.8227657434631048 1102 84-85 1.7627155364992575 1102 86-87 1.9229123639719639 1102 88-89 1.9727685405842088 1102 90-91 1.4862887881890998 1102 92-93 1.1604092936381214 1102 94-95 1.5235472339945701 1102 96-97 1.8032069311791048 1102 98-99 1.3832737951138512 1102 100-101 1.212235290418544 1103 1 0.6365459537873122 1103 2 0.6747973590434704 1103 3 0.5286022784686359 1103 4 0.5657131911168634 1103 5 0.2603847855531001 1103 6 0.4741926289393277 1103 7 0.48669820293082466 1103 8 0.40179292366873653 1103 9 0.6523965186100682 1103 10-11 0.6665369325088051 1103 12-13 0.5208464850482244 1103 14-15 0.8878296585551766 1103 16-17 0.7497035993837358 1103 18-19 0.7981234802481154 1103 20-21 0.8911612468084158 1103 22-23 0.975414182875852 1103 24-25 1.0061006098905096 1103 26-27 0.7934343094235743 1103 28-29 1.0342512484732396 1103 30-31 1.3135800499547088 1103 32-33 1.3581691958306763 1103 34-35 1.283692563039608 1103 36-37 1.3432909196141836 1103 38-39 1.497994418984561 1103 40-41 1.8643726924086081 1103 42-43 1.9972506000101546 1103 44-45 2.0103554582391325 1103 46-47 2.249060989834483 1103 48-49 2.3781910782313425 1103 50-51 2.1290368569086944 1103 52-53 1.9680155200244833 1103 54-55 2.2546002735123842 1103 56-57 2.276868238470602 1103 58-59 2.2367206321852855 1103 60-61 2.150033327559509 1103 62-63 2.35930913604496 1103 64-65 2.48668143977876 1103 66-67 2.470092612151717 1103 68-69 2.2759207473963663 1103 70-71 2.23332370767816 1103 72-73 2.3858611541856227 1103 74-75 2.394383636895345 1103 76-77 1.9425966749359276 1103 78-79 1.9514005777644527 1103 80-81 2.102392472291701 1103 82-83 2.356720790611927 1103 84-85 2.4557458351802417 1103 86-87 2.700519902042661 1103 88-89 2.8032839482744194 1103 90-91 2.525601003824999 1103 92-93 2.5511201859493475 1103 94-95 2.6311785976505035 1103 96-97 2.5590772109144417 1103 98-99 2.7936264424808392 1103 100-101 3.1565645140776333 1104 1 0.5131723210872536 1104 2 0.49627116445509856 1104 3 0.5648861011514263 1104 4 0.4073510725215854 1104 5 0.3870255454840148 1104 6 0.39831467844997803 1104 7 0.5872883008007221 1104 8 0.695834374445937 1104 9 0.6025980154436894 1104 10-11 0.6648889762624037 1104 12-13 0.7048274867753435 1104 14-15 0.6362032912552351 1104 16-17 0.9899238066376199 1104 18-19 0.9981594618255443 1104 20-21 0.7208100666126782 1104 22-23 0.9214849946202364 1104 24-25 1.046817362912961 1104 26-27 0.798500515526051 1104 28-29 0.9299406554968357 1104 30-31 1.0046566187514827 1104 32-33 1.0137895182256145 1104 34-35 1.0311018894644803 1104 36-37 0.7099935678582838 1104 38-39 0.7978576889903195 1104 40-41 1.2457918058225417 1104 42-43 1.1799938354735886 1104 44-45 1.2568220673352783 1104 46-47 0.5586968562708705 1104 48-49 0.612071331541646 1104 50-51 0.7885863675592368 1104 52-53 0.6033638216940282 1104 54-55 0.3875809873868832 1104 56-57 0.5805239667377258 1104 58-59 0.9541141267160889 1104 60-61 0.6516021243355645 1104 62-63 0.43728841065636104 1104 64-65 0.43971828491405063 1104 66-67 0.5539009023071593 1104 68-69 0.6672132056577382 1104 70-71 0.4994866322607727 1104 72-73 0.3214181490042769 1104 74-75 0.39244782802948563 1104 76-77 0.4729363410268874 1104 78-79 0.4606046652716458 1104 80-81 0.6377839518541641 1104 82-83 0.6300872269965012 1104 84-85 0.38143668146694054 1104 86-87 0.7261171962280422 1104 88-89 0.8553708797654949 1104 90-91 0.33993606427404544 1104 92-93 0.32881880425677323 1104 94-95 0.3639865999533214 1104 96-97 0.17939960584823567 1104 98-99 0.5221742260156681 1104 100-101 0.5804562814927188 1105 1 0.28338707365495 1105 2 0.20756901692942265 1105 3 0.3514640656705659 1105 4 0.3216601294776993 1105 5 0.11398632979773993 1105 6 0.28617648984120336 1105 7 0.339225742444043 1105 8 0.2861705088997155 1105 9 0.44340100330550314 1105 10-11 0.500871702686311 1105 12-13 0.4280067584840239 1105 14-15 0.5393078664186319 1105 16-17 0.43666750411661326 1105 18-19 0.5223074543558894 1105 20-21 0.38416207408233305 1105 22-23 0.4310788321552508 1105 24-25 0.6995484553499338 1105 26-27 0.7166611130984109 1105 28-29 0.39381787305052285 1105 30-31 0.12841945722020398 1105 32-33 0.2619804612694949 1105 34-35 0.1193371835821253 1105 36-37 0.1746177508648188 1105 38-39 -0.004745018759450659 1105 40-41 0.1733594995667005 1105 42-43 0.12161381679946004 1105 44-45 -0.5616536562875041 1105 46-47 -0.8010253659513538 1105 48-49 -0.7502069130895386 1105 50-51 -0.9333207286125607 1105 52-53 -0.812415823497382 1105 54-55 -1.027416678346075 1105 56-57 -0.7185890117870173 1105 58-59 -0.4941468443390029 1105 60-61 -0.686329715066865 1105 62-63 -0.8293782560103082 1105 64-65 -0.7047961875415965 1105 66-67 -0.5219394338272956 1105 68-69 -0.3890659726802639 1105 70-71 -0.3317225180660266 1105 72-73 -0.5099894140209322 1105 74-75 -0.2650334978341924 1105 76-77 -0.011750867189732617 1105 78-79 0.07455191083654 1105 80-81 0.13201831226499294 1105 82-83 -0.013248440603870648 1105 84-85 0.2752041884697398 1105 86-87 0.17813633721776867 1105 88-89 0.5272429619316945 1105 90-91 0.6956783611928152 1105 92-93 0.6562347706433265 1105 94-95 0.8421261891223253 1105 96-97 0.632947691749262 1105 98-99 0.8861214715805623 1105 100-101 1.1050594560958942 1106 1 0.4058754719993587 1106 2 0.5344137846872705 1106 3 0.5252924029756407 1106 4 0.39263299623634396 1106 5 0.19909022210092786 1106 6 0.051216834337211026 1106 7 0.23111085469790282 1106 8 0.2667298968339935 1106 9 0.2505250469528093 1106 10-11 0.3341883129124881 1106 12-13 0.5823150489643965 1106 14-15 0.6050673045222297 1106 16-17 0.855782015592851 1106 18-19 0.6245483507144343 1106 20-21 0.6734634662809995 1106 22-23 0.8589228055655056 1106 24-25 0.7220661191318669 1106 26-27 0.9010709964547416 1106 28-29 0.5479547516825818 1106 30-31 0.6445405325159896 1106 32-33 0.5352862014760262 1106 34-35 0.7430421879719376 1106 36-37 0.8973277303790113 1106 38-39 1.0822067768841848 1106 40-41 0.8448382403167329 1106 42-43 1.0267185452580065 1106 44-45 1.4420003426254908 1106 46-47 1.2566860768347112 1106 48-49 1.0480580645433086 1106 50-51 0.9293616577748267 1106 52-53 0.7448231915116104 1106 54-55 1.0130992295096028 1106 56-57 1.0207395554607785 1106 58-59 1.03199555291841 1106 60-61 1.087381560488133 1106 62-63 0.905945127074272 1106 64-65 1.069776328031793 1106 66-67 1.0833785142474568 1106 68-69 1.0281170199197618 1106 70-71 1.3253158196571206 1106 72-73 1.1917539698997999 1106 74-75 1.484964411777412 1106 76-77 1.4825549148245685 1106 78-79 1.3381958758852477 1106 80-81 1.9403129734130324 1106 82-83 2.1825540595006494 1106 84-85 1.8768346914171907 1106 86-87 1.9437996174552374 1106 88-89 2.325478674127023 1106 90-91 2.397149994622307 1106 92-93 2.353880993311499 1106 94-95 2.487763548543704 1106 96-97 1.8469783786508884 1106 98-99 2.2089694167287703 1106 100-101 2.172081488789569 1107 1 0.11486314234329242 1107 2 0.2666817924744258 1107 3 0.32261556975045735 1107 4 0.1697906860481595 1107 5 0.34698931359995555 1107 6 0.2898605721697862 1107 7 0.3993052090132778 1107 8 0.27735611357637424 1107 9 0.40483231496060057 1107 10-11 0.47823438689042064 1107 12-13 0.4046463273550529 1107 14-15 -0.05923753000080012 1107 16-17 0.520358589246321 1107 18-19 0.4981896550622622 1107 20-21 0.5133523371440774 1107 22-23 0.5569922409970474 1107 24-25 0.6186470730578932 1107 26-27 0.3141104189076884 1107 28-29 0.3494756796514267 1107 30-31 0.501516522133123 1107 32-33 0.5071470061483225 1107 34-35 0.5854497155514338 1107 36-37 0.47134622486311883 1107 38-39 0.833395128603847 1107 40-41 0.8140587140350988 1107 42-43 0.2436109852320456 1107 44-45 0.21385709148987075 1107 46-47 -0.04468478624121275 1107 48-49 -0.6685748037240522 1107 50-51 -0.7981286082861736 1107 52-53 -0.4165939077745726 1107 54-55 -0.4636569353184292 1107 56-57 -0.38662579171638 1107 58-59 -0.38444263656893796 1107 60-61 -0.4202879722827966 1107 62-63 -0.9792876763001637 1107 64-65 -1.0641464493854684 1107 66-67 -0.8681099672580608 1107 68-69 -0.615637035888156 1107 70-71 -0.35292882667642544 1107 72-73 -0.7981470683870278 1107 74-75 -0.6920026550623 1107 76-77 -0.6885068956880858 1107 78-79 -0.6697697308636172 1107 80-81 -0.46550711094776887 1107 82-83 -0.6540009372547075 1107 84-85 -0.2418423040403077 1107 86-87 -0.13979463952075122 1107 88-89 0.004948174451484988 1107 90-91 0.10261722369433812 1107 92-93 0.022115905706048267 1107 94-95 0.2819515757987361 1107 96-97 0.9472136155100692 1107 98-99 0.5244991052427217 1107 100-101 0.736917634149723 1108 1 -0.06830916039422874 1108 2 0.25068711887105266 1108 3 0.1814815427468659 1108 4 0.006282696453212111 1108 5 0.09521642867489533 1108 6 -0.07775369761839812 1108 7 0.1665618050742239 1108 8 -0.1695502409386762 1108 9 -0.21649030364462618 1108 10-11 -0.2942634453900155 1108 12-13 -0.19681068986283634 1108 14-15 0.35621753626948305 1108 16-17 0.15221013142394924 1108 18-19 0.20859393476001742 1108 20-21 0.3161092119118294 1108 22-23 0.2598753080105496 1108 24-25 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0.6979350982322217 2206 84-85 0.700673441591551 2206 86-87 0.8112288265499537 2206 88-89 0.6035276814269714 2206 90-91 0.8633005502553566 2206 92-93 0.8647611718580173 2206 94-95 1.0230078875961084 2206 96-97 1.3364608727225864 2206 98-99 1.561361661114315 2206 100-101 1.1573514009112031 2207 1 0.4844283597345935 2207 2 0.3943991837787699 2207 3 0.2818547001852423 2207 4 0.460551555613371 2207 5 0.3524240962086509 2207 6 0.4855127460828328 2207 7 0.41289216553501973 2207 8 0.524638722272023 2207 9 0.4483105758301633 2207 10-11 0.5176365608034672 2207 12-13 0.5826354577898343 2207 14-15 0.80353420912963 2207 16-17 0.8532390240289303 2207 18-19 0.6272657420187784 2207 20-21 0.4780262501875612 2207 22-23 0.6982965888231334 2207 24-25 0.2545166382752839 2207 26-27 0.25025172325550926 2207 28-29 0.5614322013905593 2207 30-31 0.4566795656113811 2207 32-33 0.7014404844091828 2207 34-35 0.7376236285949176 2207 36-37 0.9645527466022443 2207 38-39 0.6635581720821051 2207 40-41 0.8249282792524895 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56-57 -1.2126284880426361 2316 58-59 -1.2351300593304266 2316 60-61 -1.3218474880675366 2316 62-63 -0.8112904007131512 2316 64-65 -1.2093127228378862 2316 66-67 -1.3371197695274688 2316 68-69 -0.9844017814223669 2316 70-71 -1.1628518690312184 2316 72-73 -0.9723480008665248 2316 74-75 -1.3685663838568196 2316 76-77 -2.264014563365876 2316 78-79 -2.400151148681843 2316 80-81 -1.7135726372931224 2316 82-83 -1.8452680701611222 2316 84-85 -1.796018098894816 2316 86-87 -1.521524922635006 2316 88-89 -1.8105851925859255 2316 90-91 -1.906833961565745 2316 92-93 -2.5161101363117027 2316 94-95 -2.8408260615453855 2316 96-97 -2.8380422546168838 2316 98-99 -2.9865144044752903 2316 100-101 -2.9093370001610275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 924.0 3 258.0 4 258.0 5 268.0 6 327.0 7 471.0 8 598.0 9 833.0 10 1142.0 11 1736.0 12 2936.0 13 4120.0 14 5086.0 15 6963.0 16 9639.0 17 13341.0 18 10476.0 19 4753.0 20 3649.0 21 3097.0 22 3228.0 23 3432.0 24 3791.0 25 3969.0 26 4769.0 27 5276.0 28 6406.0 29 7528.0 30 8960.0 31 10966.0 32 13921.0 33 17854.0 34 24461.0 35 35746.0 36 44493.0 37 48549.0 38 36995.0 39 5741.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.35740213872265 18.538212498773667 15.17540048492663 16.92898487757705 2 17.35925553122468 22.49023571666896 37.75532234051203 22.395186411594334 3 21.47732656087504 28.857792391636462 32.20589842994458 17.45898261754392 4 12.088269610482858 18.215143152037335 37.92547343385388 31.771113803625923 5 11.44906525939157 39.693457797169565 31.136522321891746 17.720954621547126 6 25.484154065716535 36.87225398356386 17.671798639013268 19.97179331170634 7 23.564925032036992 38.98670569992064 19.844427806706875 17.603941461335488 8 27.38814570149195 27.859199712881367 19.403219429856414 25.349435155770273 9 29.14978167530898 15.958875873228653 22.222004100073196 32.66933835138917 10-11 25.67593146952963 26.647466843148003 24.02324169483778 23.65335999248459 12-13 31.067871336749214 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19.225433234396412 38-39 19.588352681999854 24.814174322983526 36.75130190085934 18.84617109415728 40-41 19.130170316301705 25.72964669738863 35.817720071310845 19.322462914998823 42-43 18.711502102974997 25.839613293661085 34.17779353704838 21.271091066315538 44-45 19.863145194176592 24.762367871211303 33.82272776319606 21.55175917141604 46-47 21.20176941798927 26.315398616468705 32.546333726132545 19.936498239409485 48-49 20.737604872149518 26.360167431093025 30.885207123809046 22.017020572948415 50-51 19.88037392605545 27.397005004735725 31.00498231809495 21.717638751113878 52-53 21.35480598386889 28.641270535218844 27.20359502951018 22.800328451402084 54-55 20.736034349390703 26.354805439400902 28.505145682223294 24.4040145289851 56-57 22.78747636858974 26.82299951090513 26.431303192570244 23.958220927934885 58-59 23.610202105280862 26.256168198234437 26.855434671405487 23.278195025079217 60-61 22.95789807078371 26.765178248597866 28.627686201745068 21.649237478873356 62-63 23.56475440738088 26.85927074121362 28.062181946100946 21.513792905304555 64-65 23.074034267105176 28.696843878261674 27.543723699569384 20.685398155063766 66-67 23.824219872320445 27.299178001555703 26.270313452603695 22.606288673520158 68-69 23.331128445021204 27.020766123946487 27.726096815150946 21.92200861588136 70-71 24.024065257535803 27.427752386770273 27.205258040200302 21.342924315493622 72-73 22.786815884152833 26.64661346543975 29.12374889319779 21.442821757209625 74-75 20.873563991441745 29.690470886104325 28.00306012215268 21.432905000301247 76-77 20.482785390879318 28.194011006027107 28.38949620870813 22.93370739438544 78-79 20.421360676554105 27.790967879377376 29.323978483471574 22.463692960596944 80-81 20.77758470894874 27.892716011322555 28.322636697401975 23.00706258232673 82-83 19.98444321422815 27.472302614521066 28.799674849162244 23.743579322088536 84-85 20.294323756131742 27.454099509460406 27.73679046951647 24.51478626489138 86-87 19.68745182132896 28.28028423663962 27.399823403271245 24.63244053876018 88-89 19.6576271946795 27.373562348243972 26.767239203943273 26.201571253133256 90-91 19.71842830012824 26.547768379596214 27.67811967848408 26.05568364179146 92-93 20.608559870595524 26.954733663946445 28.688770461241734 23.7479360042163 94-95 20.067929944966373 26.91671502785324 28.7231805680547 24.292174459125686 96-97 18.25330290251671 27.596759903099905 29.82649948409672 24.32343771028666 98-99 19.679770246791183 27.570385058188535 27.654523196183494 25.095321498836793 100-101 20.06983103133983 26.464278202341724 29.283460702517 24.182430063801444 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2001.0 1 1317.0 2 637.5 3 597.5 4 503.0 5 495.0 6 721.0 7 1349.5 8 2078.5 9 2250.5 10 1691.5 11 1138.0 12 1045.0 13 1093.0 14 1171.5 15 1145.0 16 1105.5 17 1171.0 18 1224.0 19 1310.5 20 1467.0 21 1628.5 22 1817.0 23 1984.0 24 2187.0 25 2569.5 26 3119.0 27 3787.5 28 4182.5 29 4418.0 30 4782.5 31 5210.5 32 5893.0 33 6649.5 34 7512.0 35 8009.0 36 8113.5 37 8637.0 38 8150.5 39 7007.0 40 6755.0 41 7025.5 42 7404.0 43 8077.0 44 8767.0 45 8718.0 46 9270.0 47 14033.5 48 23851.0 49 23456.0 50 13828.5 51 8899.0 52 7152.0 53 6784.0 54 6338.0 55 6178.0 56 6686.0 57 6909.5 58 7318.5 59 8046.5 60 8913.5 61 9817.5 62 8540.0 63 4902.5 64 2338.0 65 1613.0 66 1159.0 67 859.0 68 645.5 69 453.5 70 352.0 71 343.0 72 311.0 73 300.5 74 243.5 75 176.5 76 132.0 77 70.0 78 38.0 79 23.5 80 16.0 81 9.5 82 7.5 83 10.0 84 6.0 85 3.5 86 3.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05770938561915727 2 0.08516336518555248 3 0.06219166799734425 4 0.11681948448149797 5 0.0946882152391998 6 0.08628393578009923 7 0.09580878583374654 8 0.08796479167191934 9 0.10701449177921397 10-11 0.10099142483352523 12-13 0.08222186737486728 14-15 0.08530343650987082 16-17 0.09987085423897848 18-19 0.08012079751009214 20-21 0.06695409302416791 22-23 0.07059594745644482 24-25 0.06051081210552413 26-27 0.05084589072755847 28-29 0.0610710974027975 30-31 0.04552318040346144 32-33 0.04818453556550996 34-35 0.044402609808914695 36-37 0.04706396497096321 38-39 0.049024963511420015 40-41 0.05967038415961407 42-43 0.05728917164620225 44-45 0.044682752457551385 46-47 0.03375718916072064 48-49 0.04412246716027802 50-51 0.027734122215031895 52-53 0.03781925756595258 54-55 0.04398239583595967 56-57 0.0502856054302851 58-59 0.053647317213925336 60-61 0.05154624734915019 62-63 0.056448743700292195 64-65 0.04104089802527447 66-67 0.058409742240748984 68-69 0.04986539145733007 70-71 0.027453979566395205 72-73 0.021570983945024807 74-75 0.03151604797162715 76-77 0.04384232451164133 78-79 0.05939024151097739 80-81 0.04230153994413956 82-83 0.057009028997565554 84-85 0.0591100988623407 86-87 0.06051081210552413 88-89 0.0848832225369158 90-91 0.05981045548393241 92-93 0.07003566215917145 94-95 0.07563851513190516 96-97 0.08544350783418918 98-99 0.11331770137353941 100-101 0.10813506237376072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 356961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.99520510882805 #Duplication Level Percentage of deduplicated Percentage of total 1 78.55605658814282 32.204216523716184 2 11.62387890853383 9.530466000310495 3 3.91958697088362 4.820528154397921 4 1.8095297893873046 2.967281794658679 5 0.9481587688615005 1.943498160260555 6 0.5638554044355535 1.386922077393403 7 0.3866572027552562 1.1095763933630212 8 0.2829464960846217 0.9279559713450625 9 0.22739560452765123 0.838991650361131 >10 1.364177168589651 11.010832320262516 >50 0.14723658626426436 4.22014244702871 >100 0.1438124796069559 11.55120815480284 >500 0.0130116052977722 3.73950887403023 >1k 0.012326783966310506 10.183423827905296 >5k 0.0013696426629233895 3.565447650163958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6831 1.913654432837201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5869 1.644157204848709 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4894 1.3710181224279403 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3850 1.0785491972512404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3276 0.9177473169337826 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2778 0.7782362779127132 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2328 0.6521720860262046 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2155 0.6037074078120579 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1790 0.5014553410596676 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1585 0.444026098089147 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1554 0.43534167598140977 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1489 0.41713240382002514 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1480 0.414611119982295 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1460 0.4090082670095613 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1457 0.40816783906365117 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1403 0.3930401360372702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.3921997080913601 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1218 0.3412137460394833 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1128 0.3160009076621816 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1028 0.287986642798513 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 955 0.26753622944803496 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 951 0.26641565885348817 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 867 0.2428836763680066 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 838 0.2347595395575427 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 838 0.2347595395575427 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 825 0.23111768512526581 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 825 0.23111768512526581 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 737 0.20646513204523745 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 723 0.20254313496432383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 681 0.19077714372158303 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 655 0.1834934348570292 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 593 0.1661245906415547 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 581 0.16276287885791446 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 565 0.15828059647972748 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 559 0.15659974058790735 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 551 0.15435859939881388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 542 0.1518373155610837 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 523 0.14651460523698667 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 511 0.14315289345334645 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 489 0.13698975518333936 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 470 0.13166704485924233 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 459 0.12858547572423878 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 451 0.1263443345351453 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 444 0.1243833359946885 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 441 0.12354290804877843 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 419 0.11737976977877135 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 417 0.11681948448149797 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.1162591991842246 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 411 0.11513862858967785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 406 0.11373791534649443 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 405 0.11345777269785776 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 400 0.11205705945467433 No Hit GTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAG 396 0.11093648886012758 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 385 0.10785491972512404 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 385 0.10785491972512404 No Hit GAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCC 377 0.10561377853603054 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 365 0.10225206675239032 No Hit GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA 364 0.10197192410375364 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 363 0.10169178145511694 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.2021397295502873E-4 0.0 12-13 0.0 0.0 0.0 5.602852972733716E-4 0.0 14-15 0.0 0.0 0.0 5.602852972733716E-4 0.0 16-17 0.0 0.0 0.0 5.602852972733716E-4 0.0 18-19 0.0 0.0 0.0 7.003566215917145E-4 0.0 20-21 0.0 0.0 0.0 9.804992702284003E-4 0.0 22-23 0.0 0.0 0.0 0.0019609985404568007 0.0 24-25 0.0 0.0 0.0 0.002661355162048515 0.0 26-27 0.0 0.0 0.0 0.0070035662159171445 0.0 28-29 0.0 0.0 0.0 0.015687988323654405 0.0 30-31 0.0 0.0 0.0 0.034877759755267385 0.0 32-33 0.0 0.0 0.0 0.05406753118688036 0.0 34-35 0.0 0.0 0.0 0.07535837248326849 0.0 36-37 0.0 0.0 0.0 0.09384778729328974 0.0 38-39 0.0 0.0 0.0 0.11093648886012758 0.0 40-41 0.0 0.0 0.0 0.12298262275150507 0.0 42-43 0.0 0.0 0.0 0.13825039710220444 0.0 44-45 0.0 0.0 0.0 0.15295788615563044 0.0 46-47 0.0 0.0 0.0 0.1799916517490706 0.0 48-49 0.0 0.0 0.0 0.2063250607209191 0.0 50-51 0.0 0.0 0.0 0.2315378990982208 0.0 52-53 0.0 0.0 0.0 0.2755202949341805 0.0 54-55 0.0 0.0 0.0 0.3646056572006466 0.0 56-57 0.0 0.0 0.0 0.5161628301130936 0.0 58-59 0.0 0.0 0.0 0.6653387905121289 0.0 60-61 0.0 0.0 0.0 0.8019083317225131 0.0 62-63 0.0 0.0 0.0 0.9706942775261163 0.0 64-65 0.0 0.0 0.0 1.1663739175988415 0.0 66-67 0.0 0.0 0.0 1.459963413370088 0.0 68-69 0.0 0.0 0.0 1.827090354408465 0.0 70-71 0.0 0.0 0.0 2.1415504775031446 0.0 72-73 0.0 0.0 0.0 2.451808460868274 0.0 74-75 0.0 0.0 0.0 2.802406985637086 0.0 76-77 0.0 0.0 0.0 3.175977207594107 0.0 78-79 0.0 0.0 0.0 3.4715277019058104 0.0 80-81 0.0 0.0 0.0 3.7899098220814036 0.0 82-83 0.0 0.0 0.0 4.1549356932550054 0.0 84-85 0.0 0.0 0.0 4.724325626609069 0.0 86-87 0.0 0.0 0.0 5.4022708363098495 0.0 88-89 0.0 2.801426486366858E-4 0.0 6.10206717260429 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTGTA 25 0.0050112065 57.020786 9 CGTCACT 185 0.0 46.22659 1 CGCAGAA 90 5.6670797E-7 36.957916 9 GGATAAT 70 2.0478015E-4 33.936188 1 CTTTGCG 145 6.5847416E-10 32.770565 2 ATACTTA 60 0.0039402894 31.678215 6 CCTAAGA 90 2.4023368E-5 31.678213 2 CTAGAAG 60 0.0039429963 31.673775 3 TTTGCGT 160 1.891749E-9 29.69416 3 GCTTTGC 160 1.891749E-9 29.69416 1 GGATGAC 65 0.0058283443 29.241428 8 GTCACTA 315 0.0 28.66124 2 TTGCGTT 170 3.608875E-9 27.951366 4 TTAATAC 170 3.614332E-9 27.947445 3 GATGTAA 70 0.008369045 27.152756 9 ACTACCT 340 0.0 26.553797 5 TAATACC 130 9.967565E-6 25.586248 4 TCACTAC 355 0.0 25.428238 3 TCCTAGG 95 0.0012222945 25.009117 2 TAAGAGG 115 1.2761683E-4 24.791645 4 >>END_MODULE