##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937031_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356961 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.903628127442495 31.0 31.0 34.0 30.0 34.0 2 31.805205610696966 31.0 31.0 34.0 30.0 34.0 3 31.917825756875402 31.0 31.0 34.0 30.0 34.0 4 35.760971086477234 37.0 35.0 37.0 35.0 37.0 5 35.71826053826609 37.0 35.0 37.0 33.0 37.0 6 35.852776633861964 37.0 35.0 37.0 35.0 37.0 7 35.7900134748614 37.0 35.0 37.0 35.0 37.0 8 35.7954790579363 37.0 35.0 37.0 35.0 37.0 9 37.353904208022726 39.0 37.0 39.0 34.0 39.0 10-11 37.29714590669569 39.0 37.0 39.0 34.0 39.0 12-13 37.19249581887097 39.0 37.0 39.0 33.0 39.0 14-15 37.99714954855013 40.0 37.0 41.0 33.0 41.0 16-17 38.00536473172139 39.5 37.0 41.0 33.0 41.0 18-19 38.24684069128 40.0 37.5 41.0 33.5 41.0 20-21 38.3834074310639 40.0 38.0 41.0 34.0 41.0 22-23 38.2781256215665 40.0 37.5 41.0 34.0 41.0 24-25 38.0882561400265 40.0 37.0 41.0 33.0 41.0 26-27 37.7952003160009 40.0 36.5 41.0 33.0 41.0 28-29 37.538493000635924 39.5 36.0 41.0 33.0 41.0 30-31 37.09690274287668 39.0 35.5 41.0 32.0 41.0 32-33 36.616980566504466 38.0 35.0 41.0 31.5 41.0 34-35 36.26353299099902 38.0 35.0 41.0 31.0 41.0 36-37 35.85217012502767 38.0 35.0 40.0 30.5 41.0 38-39 35.455967178487285 38.0 35.0 40.0 30.0 41.0 40-41 35.0484954939055 38.0 35.0 40.0 28.0 41.0 42-43 34.42095635097392 38.0 34.5 40.0 24.0 41.0 44-45 33.42687296371312 38.0 33.0 40.0 13.0 41.0 46-47 32.23213880507954 37.0 33.0 40.0 2.0 41.0 48-49 31.166490176798025 37.0 31.0 40.0 2.0 41.0 50-51 30.53949310989156 36.5 30.0 40.0 2.0 41.0 52-53 30.157927616742445 36.0 29.0 40.0 2.0 41.0 54-55 29.838500564487436 35.5 28.5 40.0 2.0 41.0 56-57 29.450875585848316 35.0 27.5 39.0 2.0 41.0 58-59 29.098300654693368 35.0 27.0 39.0 2.0 40.5 60-61 28.66300380153574 35.0 26.0 39.0 2.0 40.0 62-63 28.307280067010122 35.0 26.0 38.0 2.0 40.0 64-65 27.912348968094555 34.5 24.5 37.5 2.0 40.0 66-67 27.506121116872713 34.0 23.0 37.0 2.0 39.5 68-69 27.056853549827572 34.0 22.0 36.5 2.0 39.0 70-71 26.571824372970717 33.5 20.5 36.0 2.0 39.0 72-73 26.286648961651274 33.0 20.0 35.0 2.0 38.0 74-75 25.927008832897712 33.0 18.5 35.0 2.0 37.0 76-77 24.694926056347892 31.5 16.0 34.0 2.0 36.0 78-79 25.3396995750236 33.0 16.0 35.0 2.0 36.0 80-81 25.146303097537263 33.0 9.5 35.0 2.0 36.0 82-83 24.82010499746471 33.0 6.0 35.0 2.0 35.5 84-85 24.473443877622486 33.0 2.0 35.0 2.0 35.0 86-87 24.164998417194035 32.0 2.0 35.0 2.0 35.0 88-89 23.793516658682602 32.0 2.0 35.0 2.0 35.0 90-91 23.324767971851266 31.5 2.0 34.5 2.0 35.0 92-93 22.893711077680756 31.0 2.0 34.0 2.0 35.0 94-95 22.41438000229717 31.0 2.0 34.0 2.0 35.0 96-97 21.80392675950594 31.0 2.0 34.0 2.0 35.0 98-99 21.193909138533343 30.0 2.0 34.0 2.0 35.0 100-101 18.830372225537243 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4015644780516112 1101 2 0.25129971907049864 1101 3 0.22285417043304534 1101 4 0.35925767668573627 1101 5 0.09114239364683385 1101 6 0.24456971573421527 1101 7 0.44013573000557216 1101 8 0.4004918399715294 1101 9 0.38957283225608563 1101 10-11 0.45754650953904275 1101 12-13 0.4360473050912006 1101 14-15 0.5767714662859547 1101 16-17 0.5999319807953043 1101 18-19 0.3669741124131747 1101 20-21 0.5727734074195681 1101 22-23 0.3744485385683376 1101 24-25 0.5593239471085738 1101 26-27 0.44938066423311795 1101 28-29 0.507232483020907 1101 30-31 0.846633806142485 1101 32-33 0.5817267730386817 1101 34-35 0.24966523941093755 1101 36-37 0.029198202674272977 1101 38-39 0.40584614570590816 1101 40-41 0.2721377581188875 1101 42-43 0.0986412579656033 1101 44-45 0.529660221961187 1101 46-47 0.6998201558974984 1101 48-49 -0.1085443595632718 1101 50-51 -0.3207672859110282 1101 52-53 -0.33414676013428135 1101 54-55 -0.11931586119666449 1101 56-57 -0.22510256703127496 1101 58-59 -0.06823434258381411 1101 60-61 0.08992382961034551 1101 62-63 0.06350594978827573 1101 64-65 -0.15886544390740198 1101 66-67 -0.011420131388891974 1101 68-69 -0.008931343472458053 1101 70-71 -6.351244058109273E-4 1101 72-73 0.3732806768833896 1101 74-75 0.3027497147755476 1101 76-77 -0.0679987138563618 1101 78-79 0.15504390661101652 1101 80-81 0.37838564626856197 1101 82-83 0.6791395974237204 1101 84-85 0.5441018440496457 1101 86-87 0.7116768905325443 1101 88-89 0.7175364140299294 1101 90-91 0.8392204636006362 1101 92-93 0.7265108617100111 1101 94-95 1.181783332401082 1101 96-97 0.826610915167052 1101 98-99 1.0311320548598744 1101 100-101 1.1465047699211866 1102 1 0.13002023282315633 1102 2 -0.005587438093666464 1102 3 0.0828356017441223 1102 4 0.1998256959241118 1102 5 -0.10140882245008953 1102 6 0.13454262371266879 1102 7 0.13748132070296748 1102 8 0.014369651910286052 1102 9 0.2739218627270006 1102 10-11 0.3420325221413947 1102 12-13 0.25581687070564385 1102 14-15 0.41968720705487783 1102 16-17 0.5358509786949099 1102 18-19 0.2586402717386065 1102 20-21 0.2997489941596925 1102 22-23 0.25936071780184733 1102 24-25 0.2821634344909967 1102 26-27 0.01511306202922924 1102 28-29 -0.05466061003562572 1102 30-31 0.07611692689984295 1102 32-33 0.33574491556097996 1102 34-35 0.0021423329545129377 1102 36-37 0.1448225367889151 1102 38-39 0.20146880559449443 1102 40-41 -0.1027252597496755 1102 42-43 0.01644056392280646 1102 44-45 0.628340627428301 1102 46-47 0.2775993711420881 1102 48-49 0.33589036084097756 1102 50-51 0.1404896012461343 1102 52-53 0.4077674890514338 1102 54-55 0.36947062932112473 1102 56-57 0.3053721521553534 1102 58-59 0.5946488268783021 1102 60-61 0.24633459336182995 1102 62-63 0.6670644411584021 1102 64-65 0.8065123223706365 1102 66-67 0.8175196269137466 1102 68-69 0.5818954197919766 1102 70-71 0.3436884882696063 1102 72-73 0.4331532835011487 1102 74-75 0.35487141578921566 1102 76-77 0.2725807205596382 1102 78-79 0.9129470145833771 1102 80-81 0.8527091067416066 1102 82-83 0.8192685128296446 1102 84-85 0.6154238738204292 1102 86-87 0.3954308097435266 1102 88-89 0.6099940256703675 1102 90-91 1.066534700610159 1102 92-93 1.2167204443711164 1102 94-95 0.8699967810023423 1102 96-97 1.1728560827418626 1102 98-99 1.3518604953944084 1102 100-101 1.3097341997711176 1103 1 0.1758342497307055 1103 2 -0.06459164345298873 1103 3 0.28937184964502904 1103 4 0.2005433340335685 1103 5 0.3314202505050048 1103 6 0.35323566118772476 1103 7 0.3768208065870269 1103 8 0.44071845273700205 1103 9 0.2069303452094502 1103 10-11 0.4034982262161435 1103 12-13 0.506764153010117 1103 14-15 0.682611869406152 1103 16-17 0.761357990019242 1103 18-19 0.6872568089912079 1103 20-21 0.8200617408477981 1103 22-23 0.7596190675212497 1103 24-25 0.8539774801040707 1103 26-27 0.8552125266711954 1103 28-29 0.6739465979215495 1103 30-31 0.6786243544542785 1103 32-33 0.7691947454807462 1103 34-35 0.6251634458676918 1103 36-37 0.7439750061816497 1103 38-39 0.7764548116513552 1103 40-41 0.6604469877493528 1103 42-43 0.36577244284290344 1103 44-45 0.5609902908560258 1103 46-47 0.6685399076483378 1103 48-49 0.5657211417747696 1103 50-51 0.6444294344670389 1103 52-53 0.716990574034142 1103 54-55 0.9299567573764698 1103 56-57 1.0188264314313997 1103 58-59 1.1475531083072603 1103 60-61 1.180412356627368 1103 62-63 1.3156980581980022 1103 64-65 1.4322138136226314 1103 66-67 1.6561622861935241 1103 68-69 1.3262619606327632 1103 70-71 1.3071212384023667 1103 72-73 1.4212010207881889 1103 74-75 1.0359530399477848 1103 76-77 1.0750221254079975 1103 78-79 1.1349519519335693 1103 80-81 1.3791827765993716 1103 82-83 1.1606561378780853 1103 84-85 1.4072801165543432 1103 86-87 1.4479690957366635 1103 88-89 1.6804912053525953 1103 90-91 2.046189660207137 1103 92-93 1.940462065946189 1103 94-95 2.291203704774823 1103 96-97 2.107371605254169 1103 98-99 2.11988397651335 1103 100-101 1.9613854850609727 1104 1 0.19127754276467357 1104 2 0.043943186713967464 1104 3 0.2661997137785903 1104 4 0.010906028334083828 1104 5 -0.13575015824572034 1104 6 0.10394090010539969 1104 7 0.05399409386394183 1104 8 0.0888618033414943 1104 9 -0.07987161218835581 1104 10-11 0.12856299305552454 1104 12-13 0.25710957615347496 1104 14-15 0.045910660425143135 1104 16-17 0.21934590020922684 1104 18-19 0.22259071802977815 1104 20-21 0.13643335857951655 1104 22-23 -0.06553925141945882 1104 24-25 0.12902785431248276 1104 26-27 0.08124879610124935 1104 28-29 0.12309023637865835 1104 30-31 0.2849427194514007 1104 32-33 0.3732102895221985 1104 34-35 0.37242164966735203 1104 36-37 0.12406999754607284 1104 38-39 -0.18685837199861055 1104 40-41 -0.14295686947573927 1104 42-43 0.11234699667134151 1104 44-45 0.09678476408573289 1104 46-47 0.1459434770208219 1104 48-49 -0.15830015931907226 1104 50-51 0.12048729284355275 1104 52-53 -0.033383634371155324 1104 54-55 -0.10051244239324575 1104 56-57 0.010550668621956305 1104 58-59 -0.015731290080761795 1104 60-61 0.11293970262621755 1104 62-63 0.049189710472038684 1104 64-65 -0.03987887492083786 1104 66-67 0.1408845084157484 1104 68-69 0.05602592148480667 1104 70-71 -0.11340984968053647 1104 72-73 -0.04250076389805102 1104 74-75 0.09882724835307855 1104 76-77 0.0933871225294709 1104 78-79 -0.21679675272963905 1104 80-81 -0.11659298621006897 1104 82-83 0.06140455468867856 1104 84-85 0.1422398171876189 1104 86-87 0.058288612144263396 1104 88-89 0.20985936885288226 1104 90-91 0.24546283234300148 1104 92-93 -0.04815336295421346 1104 94-95 -0.09990164928390044 1104 96-97 0.040942416998554165 1104 98-99 -0.025690289462470872 1104 100-101 -0.47460328868687185 1105 1 0.040974088049456014 1105 2 -0.035491920662316545 1105 3 0.07735751023050597 1105 4 0.12687241488870882 1105 5 0.16903508918658616 1105 6 0.3024236265293041 1105 7 0.30772892112818084 1105 8 0.23360970250116253 1105 9 0.28102941488914013 1105 10-11 0.2064691555205087 1105 12-13 0.3800787638285428 1105 14-15 0.4932495959993801 1105 16-17 0.6415447797610483 1105 18-19 0.6300370298878519 1105 20-21 0.4155066545645738 1105 22-23 0.5580835310268526 1105 24-25 0.6630731390930578 1105 26-27 0.7076493563253408 1105 28-29 0.5894884623357086 1105 30-31 0.8344058380321684 1105 32-33 0.8496108497462842 1105 34-35 0.7380028821603517 1105 36-37 0.6367450675740827 1105 38-39 1.0062555402329494 1105 40-41 1.008105222027524 1105 42-43 0.8794805167460424 1105 44-45 0.8007763607243916 1105 46-47 0.26475766936441403 1105 48-49 0.21506585375282228 1105 50-51 0.5794158642721214 1105 52-53 0.21937080073621829 1105 54-55 0.1530123008373785 1105 56-57 0.047548801208325386 1105 58-59 -0.1755118689789903 1105 60-61 0.04720674277561088 1105 62-63 -0.26637985068575887 1105 64-65 -0.07108802524763291 1105 66-67 -0.1498601227700611 1105 68-69 -0.09808238850585482 1105 70-71 -0.3235989253107867 1105 72-73 0.04115936682090293 1105 74-75 0.004161048539824463 1105 76-77 0.21925080133432573 1105 78-79 -0.08377854544673013 1105 80-81 0.019949964303467738 1105 82-83 -0.2110510942375612 1105 84-85 -0.14475831539875017 1105 86-87 -0.16615116376609862 1105 88-89 -0.21547909986793456 1105 90-91 0.18882184261377688 1105 92-93 0.3065851991372881 1105 94-95 0.2883336448337488 1105 96-97 0.5553331733010758 1105 98-99 0.29535312790447676 1105 100-101 0.29728046481453063 1106 1 0.5788811911892182 1106 2 0.31861151175542446 1106 3 0.3762328812744471 1106 4 0.27657767012512835 1106 5 0.2793825117377011 1106 6 0.21384968949180205 1106 7 0.24064417678268057 1106 8 0.2539115545852795 1106 9 0.4246474591217577 1106 10-11 0.6179286646973168 1106 12-13 0.5695473870987442 1106 14-15 0.6314827997286301 1106 16-17 0.6985747559306219 1106 18-19 0.4902109502022469 1106 20-21 0.48293004182993116 1106 22-23 0.5487227717977916 1106 24-25 0.3482857315927461 1106 26-27 0.4435622289370684 1106 28-29 0.566974979330567 1106 30-31 0.3824136978925239 1106 32-33 0.6407476029550381 1106 34-35 0.6702450327519287 1106 36-37 0.7440948731679597 1106 38-39 0.9738796047841376 1106 40-41 0.582146778948804 1106 42-43 0.9095277595237476 1106 44-45 1.0436753113494106 1106 46-47 1.2050645383806824 1106 48-49 0.8862686632348264 1106 50-51 0.6398280888634496 1106 52-53 0.8236644584978308 1106 54-55 1.015615252465789 1106 56-57 1.2799121943267657 1106 58-59 1.1568640664698187 1106 60-61 1.0864057059429655 1106 62-63 1.0542062942199628 1106 64-65 0.8491343920775059 1106 66-67 0.9091473608203877 1106 68-69 0.9098401835080239 1106 70-71 0.9274483592746918 1106 72-73 0.8924909442279869 1106 74-75 0.8785328868273758 1106 76-77 0.598693921866122 1106 78-79 1.1714495159270761 1106 80-81 1.1104177932260804 1106 82-83 1.0935770256671198 1106 84-85 1.055714112378329 1106 86-87 0.6815473186119263 1106 88-89 1.3510077933968319 1106 90-91 1.5344968290262493 1106 92-93 1.5056194396991849 1106 94-95 1.151342131810626 1106 96-97 1.330432466749798 1106 98-99 1.5820833423783256 1106 100-101 1.5876479550942264 1107 1 0.21869300170187245 1107 2 0.11175719637580173 1107 3 -0.09944038283976653 1107 4 0.16199298485582858 1107 5 0.11201554223737276 1107 6 -0.13416296462889932 1107 7 0.12223080883978099 1107 8 -0.12345703723821799 1107 9 0.11511631051695304 1107 10-11 0.06334560175118042 1107 12-13 0.12604073557375983 1107 14-15 0.34065703723673835 1107 16-17 0.21816836397734107 1107 18-19 0.30033830257083594 1107 20-21 0.07299736824135294 1107 22-23 0.06574094181726053 1107 24-25 0.13243969006126832 1107 26-27 0.12608575262298416 1107 28-29 0.26074120256222955 1107 30-31 0.48307073877506923 1107 32-33 0.693318985174372 1107 34-35 0.6485991858992364 1107 36-37 0.34535622943013067 1107 38-39 0.787296217373374 1107 40-41 0.9866325025049321 1107 42-43 0.9357769483621681 1107 44-45 1.1433427351002265 1107 46-47 1.4441016895812027 1107 48-49 1.2947493575891436 1107 50-51 1.1714836696551423 1107 52-53 1.2710849646626556 1107 54-55 1.3582134030173805 1107 56-57 1.5036062241775134 1107 58-59 0.9605670190979829 1107 60-61 1.1584409103556865 1107 62-63 0.9633805317280775 1107 64-65 1.0970172603448631 1107 66-67 0.8721948948891765 1107 68-69 1.2617626364606487 1107 70-71 1.0471879764064234 1107 72-73 0.9933386080826239 1107 74-75 1.0103912580149164 1107 76-77 0.9589064462009702 1107 78-79 0.9141815081399258 1107 80-81 0.8721147432585319 1107 82-83 0.8024673566210438 1107 84-85 1.0567929572842338 1107 86-87 0.47993716286890375 1107 88-89 0.10548134952921373 1107 90-91 0.41735292896136045 1107 92-93 0.6691171684467534 1107 94-95 0.7820729883972604 1107 96-97 1.1726453155492784 1107 98-99 1.304110435175211 1107 100-101 1.1236756968203778 1108 1 0.19419777068490163 1108 2 -0.03319356542278484 1108 3 -0.019412821833942218 1108 4 0.059908877336937394 1108 5 0.02500482750927091 1108 6 0.0913340624985608 1108 7 0.30684309671293875 1108 8 0.1345170739967685 1108 9 0.12390331658657061 1108 10-11 0.06143336342589123 1108 12-13 0.3773374963813936 1108 14-15 0.42653744105192715 1108 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-0.6428626442025092 2316 80-81 -1.0615865262617525 2316 82-83 -0.5435050060348345 2316 84-85 -0.7489100536134181 2316 86-87 -0.9323444627911321 2316 88-89 -0.7071625949714075 2316 90-91 -1.0478511211453672 2316 92-93 -1.8098458694141613 2316 94-95 -1.4084287810668457 2316 96-97 -1.131505903414883 2316 98-99 -0.6613815039327555 2316 100-101 -1.1052557434672323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 11.0 5 24.0 6 67.0 7 157.0 8 344.0 9 587.0 10 1153.0 11 2002.0 12 3148.0 13 4247.0 14 5216.0 15 7416.0 16 10403.0 17 15125.0 18 12075.0 19 5280.0 20 3389.0 21 3128.0 22 3173.0 23 3320.0 24 3686.0 25 4272.0 26 4827.0 27 5736.0 28 6862.0 29 8229.0 30 9981.0 31 11942.0 32 14444.0 33 18614.0 34 25104.0 35 34627.0 36 45112.0 37 51071.0 38 30358.0 39 1831.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.39476146979215 19.590970988584846 15.804692709880245 16.20957483174276 2 17.407248654569702 24.10260289285024 36.57637365562027 21.91377479695979 3 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18.342356476001708 22-23 20.621020223497805 23.24945862433151 37.931874910704536 18.197646241466153 24-25 20.68942815199973 23.429323038649887 38.31244326904759 17.56880554030279 26-27 20.165230448570693 22.52616568988895 40.3079090980401 17.000694763500263 28-29 18.628835249557362 25.102674869450237 38.68153141038571 17.586958470606692 30-31 19.10619221070328 23.72008966096946 38.23003642476884 18.943681703558422 32-33 18.314988556108684 24.303771581610214 38.39334489395141 18.987894968329698 34-35 20.13654363078871 23.71107883195524 38.252667349291436 17.899710187964608 36-37 19.742596281261466 23.117167998789743 38.48238824033686 18.657847479611934 38-39 19.272786358931114 24.5990001442773 37.70582277984544 18.42239071694614 40-41 19.197021398194686 25.515344057644572 36.2668302768486 19.02080426731214 42-43 18.76897993029953 25.99353982003384 34.776555093624985 20.46092515604164 44-45 20.327095931051 24.366769899666487 34.43233756210439 20.873796607178125 46-47 21.60617211148012 26.503772826405164 32.62825998955066 19.261795072564052 48-49 21.30908041646064 26.488364959148143 30.871342907688664 21.331211716702548 50-51 20.00672344240251 27.56317234424025 31.275212909009415 21.154891304347824 52-53 21.754410539069777 28.952466330025285 27.20155760839876 22.091565522506183 54-55 20.777951004410973 26.94729381244738 28.12280694381994 24.1519482393217 56-57 22.76557597489915 27.165088525324965 26.41864634692963 23.650689152846258 58-59 23.788893044390562 26.768527817815336 26.351810374653496 23.090768763140606 60-61 22.986317718050863 27.422273768902784 28.21144224250472 21.37996627054163 62-63 23.510971786833856 27.26026092486294 27.723336723059383 21.50543056524382 64-65 23.064176568555904 29.1619100712824 27.14263903148948 20.63127432867222 66-67 23.959200024652553 27.641254927008834 26.087023512372497 22.312521535966116 68-69 23.662388888421983 27.57051890822807 26.914845039093905 21.85224716425604 70-71 24.31991775000315 28.02887154180925 26.714020178703247 20.937190529484354 72-73 23.409280005378736 26.87324385577136 28.58351472569833 21.133961413151578 74-75 21.37595441499666 30.115741421645875 27.46670140450107 21.0416027588564 76-77 20.801490363065888 28.72198565665621 27.831269610040344 22.64525437023756 78-79 20.551042133572388 28.380210667861945 28.834043030031374 22.23470416853429 80-81 20.86371850004903 28.428049139223127 28.193138999005452 22.515093361722393 82-83 20.21187485292305 27.95470590212799 28.226308004347878 23.607111240601082 84-85 20.040341784563665 28.13209132931783 27.45146379044684 24.376103095671663 86-87 19.613706973108805 28.505114061357528 26.769891051303098 25.11128791423057 88-89 19.244307795745993 28.107687890014915 26.171462989291445 26.47654132494765 90-91 19.629622885445308 26.859834631562872 27.078206132051026 26.432336350940787 92-93 20.402929171534147 27.854163339972715 27.75765419751738 23.985253290975763 94-95 19.769694405963687 27.586945894573766 27.82380683577034 24.819552863692202 96-97 17.869709431974808 28.320017032911622 28.900200584946045 24.910072950167528 98-99 19.22045759525764 28.601384495998072 26.62619448498822 25.551963423756067 100-101 19.03335831296443 27.65119096811245 27.891835862812798 25.423614856110323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2829.0 1 1779.0 2 723.0 3 665.0 4 529.0 5 508.0 6 790.5 7 1356.5 8 1857.0 9 1970.5 10 1544.0 11 1090.5 12 1038.0 13 1049.0 14 1069.0 15 1100.0 16 1122.5 17 1140.0 18 1225.5 19 1375.5 20 1503.5 21 1664.0 22 1836.0 23 2016.0 24 2272.5 25 2602.5 26 3203.0 27 3951.0 28 4203.0 29 4436.0 30 4892.5 31 5282.5 32 6000.0 33 6699.5 34 7538.5 35 8106.5 36 8253.0 37 8648.0 38 8061.5 39 7138.0 40 6908.5 41 7109.0 42 7792.0 43 8567.5 44 9221.5 45 9215.5 46 9420.5 47 14316.5 48 24417.0 49 23377.0 50 13228.0 51 8260.5 52 6350.5 53 5915.5 54 5748.5 55 5837.0 56 6460.0 57 6962.0 58 7589.5 59 8502.5 60 9105.5 61 9205.5 62 7831.0 63 4637.5 64 2275.0 65 1611.5 66 1185.0 67 903.5 68 681.5 69 500.5 70 390.5 71 347.5 72 321.0 73 315.0 74 252.0 75 195.5 76 157.0 77 76.5 78 39.5 79 29.0 80 16.5 81 10.0 82 8.5 83 4.5 84 2.5 85 2.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01876955745865795 2 0.00336171178364023 3 0.003641854432276915 4 0.0 5 5.602852972733716E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.003566215917146E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0023812125134118293 22-23 0.0 24-25 0.003641854432276915 26-27 0.0014007132431834292 28-29 0.002521283837730172 30-31 0.017088701566837835 32-33 5.602852972733716E-4 34-35 0.0015407845675017718 36-37 0.00336171178364023 38-39 0.002661355162048515 40-41 0.004202139729550287 42-43 0.0014007132431834292 44-45 4.2021397295502873E-4 46-47 1.400713243183429E-4 48-49 1.400713243183429E-4 50-51 2.801426486366858E-4 52-53 9.804992702284003E-4 54-55 0.0029414978106852008 56-57 2.801426486366858E-4 58-59 0.001260641918865086 60-61 8.404279459100575E-4 62-63 0.0 64-65 1.400713243183429E-4 66-67 0.0 68-69 0.0 70-71 1.400713243183429E-4 72-73 0.0 74-75 4.2021397295502873E-4 76-77 2.801426486366858E-4 78-79 2.801426486366858E-4 80-81 0.004482282378186973 82-83 0.0014007132431834292 84-85 0.0030815691350035436 86-87 0.0011205705945467432 88-89 9.804992702284003E-4 90-91 1.400713243183429E-4 92-93 0.0 94-95 1.400713243183429E-4 96-97 0.0014007132431834292 98-99 0.0022411411890934864 100-101 9.804992702284003E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 356961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.401890890213394 #Duplication Level Percentage of deduplicated Percentage of total 1 77.68939033900814 30.611088814647974 2 11.9168718739583 9.390945706607155 3 4.161759447370077 4.919435749697717 4 1.8812718576561398 2.9650267388078513 5 1.0072759764905053 1.984428906100602 6 0.6373852230109287 1.5068509807266566 7 0.37528250985471867 1.035078835441074 8 0.2975386932851121 0.9378869702749325 9 0.20283781444605234 0.7192974089891435 >10 1.4930613026613235 11.507607614430675 >50 0.1551410163974551 4.3566670247023005 >100 0.15229439224337332 11.814343293543285 >500 0.015656432847449595 4.0574738911914965 >1k 0.012098152654847413 8.993179173344416 >5k 0.0021349681155613085 5.2006888914947265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6432 1.8018775160311629 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6148 1.7223170038183442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5967 1.6716111844151043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3395 0.9510842921215482 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2891 0.8098923972086586 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2738 0.7670305719672458 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2697 0.7555447233731416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2665 0.7465801586167676 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1954 0.5473987354360841 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1828 0.5121007617078617 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1771 0.49613263073557057 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1576 0.44150481425141685 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1535 0.4300189656573127 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1518 0.42525654063048907 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.4162919758741151 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1349 0.37791243301088917 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1316 0.3686677256058785 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1177 0.32972789744537917 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1119 0.3134796238244514 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1057 0.2961107796089769 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 857 0.24008224988163973 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 854 0.23924182193572965 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 852 0.2386815366384563 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 851 0.2384013939898196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.23784110869254624 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 779 0.21823112328797825 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 730 0.20450413350478064 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 682 0.1910572863702197 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 656 0.1837735775056659 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 622 0.17424872745201858 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 612 0.17144730096565172 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 610 0.17088701566837836 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 601 0.16836573183064818 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 586 0.16416359210109788 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 582 0.16304302150655114 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 570 0.1596813097229109 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 560 0.15687988323654403 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 555 0.15547916999336062 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 531 0.14875574642608017 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 515 0.14427346404789318 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 514 0.1439933213992565 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 502 0.14063160961561627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 497 0.13923089637243286 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 494 0.13839046842652278 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 486 0.13614932723742929 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 435 0.12186205215695832 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.11878048302195478 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 417 0.11681948448149797 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.112337202103311 No Hit CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC 396 0.11093648886012758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 394 0.11037620356285421 No Hit GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 392 0.10981591826558082 No Hit ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA 384 0.10757477707648735 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 379 0.10617406383330392 No Hit GTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAG 377 0.10561377853603054 No Hit GGTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCA 376 0.10533363588739386 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 367 0.10281235204966369 No Hit GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT 363 0.10169178145511694 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.2021397295502873E-4 0.0 12-13 0.0 0.0 0.0 5.602852972733716E-4 0.0 14-15 0.0 0.0 0.0 5.602852972733716E-4 0.0 16-17 0.0 0.0 0.0 5.602852972733716E-4 0.0 18-19 0.0 0.0 0.0 7.003566215917145E-4 0.0 20-21 0.0 0.0 0.0 9.804992702284003E-4 0.0 22-23 0.0 0.0 0.0 0.0019609985404568007 0.0 24-25 0.0 0.0 0.0 0.0029414978106852008 0.0 26-27 0.0 0.0 0.0 0.007423780188872174 0.0 28-29 0.0 0.0 0.0 0.016248273620927775 0.0 30-31 0.0 0.0 0.0 0.03557811637685909 0.0 32-33 0.0 0.0 0.0 0.055888458403018815 0.0 34-35 0.0 0.0 0.0 0.07745944234804361 0.0 36-37 0.0 0.0 0.0 0.09608892848238323 0.0 38-39 0.0 0.0 0.0 0.11345777269785776 0.0 40-41 0.0 0.0 0.0 0.12550390658923524 0.0 42-43 0.0 0.0 0.0 0.1410518235885713 0.0 44-45 0.0 0.0 0.0 0.1567398119122257 0.0 46-47 0.0 0.0 0.0 0.18461400545157594 0.0 48-49 0.0 0.0 0.0 0.2131885556125179 0.0 50-51 0.0 0.0 0.0 0.23882160796277463 0.0 52-53 0.0 0.0 0.0 0.28378450306896275 0.0 54-55 0.0 0.0 0.0 0.3732900793083838 0.0 56-57 0.0 0.0 0.0 0.5280688926801527 0.0 58-59 0.0 0.0 0.0 0.6780852810250979 0.0 60-61 0.0 0.0 0.0 0.8163356781273023 0.0 62-63 0.0 0.0 0.0 0.9854017665795423 0.0 64-65 0.0 0.0 0.0 1.1808012640036307 0.0 66-67 0.0 0.0 0.0 1.4774723289098808 0.0 68-69 0.0 0.0 0.0 1.8490815523264446 0.0 70-71 0.0 0.0 0.0 2.1662030305831728 0.0 72-73 0.0 0.0 0.0 2.4812234389751264 0.0 74-75 0.0 0.0 0.0 2.8356038895005335 0.0 76-77 0.0 0.0 0.0 3.2116953952952842 0.0 78-79 0.0 0.0 0.0 3.5107476727149463 0.0 80-81 0.0 0.0 0.0 3.8330517899714533 0.0 82-83 0.0 0.0 0.0 4.202419872198924 0.0 84-85 0.0 0.0 0.0 4.781334655606635 0.0 86-87 0.0 0.0 0.0 5.465723146226058 0.0 88-89 0.0 0.0 0.0 6.172803191385054 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATAGG 30 1.297831E-4 63.354725 1 GATAGTC 30 1.3005335E-4 63.3281 7 TGATCGT 40 5.3853507E-4 47.49608 6 GATCGTC 45 9.6200523E-4 42.21874 7 CGTCACT 160 0.0 41.576538 1 GCAGTAC 60 8.2832936E-5 39.58006 3 CGACCGT 25 0.0016149497 38.002182 18-19 CGCTCCG 25 0.0016149497 38.002182 34-35 CATAGGC 50 0.0016149788 37.99686 2 ATAGTCA 50 0.0016149788 37.99686 8 CATTCCG 90 5.6869794E-7 36.941395 9 GTCCTAG 70 2.0464415E-4 33.940033 1 TATATGG 70 2.0515021E-4 33.92577 2 GTTCGAC 30 0.003948847 31.668486 16-17 CTTTAGG 125 2.1484266E-7 30.39749 2 CTTTGCG 145 2.392153E-8 29.480326 2 GAGTATA 65 0.0058291163 29.240644 1 AGATAGT 65 0.005841104 29.228354 6 CTGATCG 65 0.005841104 29.228354 5 CACGCAT 85 6.4015505E-4 27.93887 9 >>END_MODULE