##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937030_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 335083 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861117991661768 33.0 31.0 34.0 30.0 34.0 2 31.89550648645261 33.0 31.0 34.0 30.0 34.0 3 31.79622660654226 33.0 31.0 34.0 30.0 34.0 4 35.468415288152485 37.0 35.0 37.0 33.0 37.0 5 35.514681437136474 37.0 35.0 37.0 33.0 37.0 6 35.67749781397445 37.0 35.0 37.0 33.0 37.0 7 35.649474906217264 37.0 35.0 37.0 33.0 37.0 8 35.612087154525895 37.0 35.0 37.0 33.0 37.0 9 37.19459059397224 39.0 37.0 39.0 34.0 39.0 10-11 36.99912558977925 39.0 37.0 39.0 33.0 39.0 12-13 36.93880919055876 39.0 37.0 39.0 33.0 39.0 14-15 37.79984063649901 40.0 37.0 41.0 32.5 41.0 16-17 37.808157978769444 40.0 37.5 41.0 32.5 41.0 18-19 37.96757221345159 40.0 37.5 41.0 33.0 41.0 20-21 37.985940796757816 40.0 37.5 41.0 33.0 41.0 22-23 37.880993664256316 40.0 37.5 41.0 33.0 41.0 24-25 37.63835974967397 40.0 37.0 41.0 32.5 41.0 26-27 37.568314417621906 40.0 36.5 41.0 33.0 41.0 28-29 37.447090720806486 40.0 36.0 41.0 33.0 41.0 30-31 37.05343153785779 39.0 35.5 41.0 32.0 41.0 32-33 36.486283995308625 38.0 35.0 41.0 31.5 41.0 34-35 36.08620401512461 38.0 35.0 40.0 31.0 41.0 36-37 35.70371519892086 38.0 35.0 40.0 30.0 41.0 38-39 35.324204749271075 38.0 35.0 40.0 29.5 41.0 40-41 34.93020684427441 38.0 35.0 40.0 26.5 41.0 42-43 34.29639969798528 38.0 35.0 40.0 23.5 41.0 44-45 33.38463604539771 38.0 33.5 40.0 12.5 41.0 46-47 32.13233437685588 37.0 32.5 40.0 2.0 41.0 48-49 31.099202287194515 37.0 31.0 40.0 2.0 41.0 50-51 30.071552421340385 36.0 29.0 39.0 2.0 40.5 52-53 30.138173228722437 36.0 30.0 39.5 2.0 40.5 54-55 30.34277626737256 36.0 30.0 40.0 2.0 41.0 56-57 30.099027703583886 35.5 29.5 40.0 2.0 41.0 58-59 29.819619318198775 35.0 28.5 40.0 2.0 41.0 60-61 29.43006210401602 35.0 28.0 39.0 2.0 41.0 62-63 29.104314453433926 35.0 27.0 39.0 2.0 41.0 64-65 28.777137306279343 35.0 26.5 39.0 2.0 40.5 66-67 28.347675053643428 35.0 26.0 38.0 2.0 40.0 68-69 27.946696788556864 35.0 25.5 37.0 2.0 40.0 70-71 27.50492266095266 35.0 24.0 36.5 2.0 39.0 72-73 27.16311481036042 34.0 23.5 36.0 2.0 39.0 74-75 26.799039342491263 34.0 22.0 35.5 2.0 38.5 76-77 26.46406711173053 34.0 20.0 35.0 2.0 37.0 78-79 26.043231080060764 34.0 18.0 35.0 2.0 37.0 80-81 25.609414682332435 34.0 13.5 35.0 2.0 36.0 82-83 25.226912436620182 33.0 7.5 35.0 2.0 36.0 84-85 24.90208097695198 33.0 3.5 35.0 2.0 35.5 86-87 24.649780800577766 33.0 2.0 35.0 2.0 35.0 88-89 24.260993544882908 33.0 2.0 35.0 2.0 35.0 90-91 23.841133390831523 32.5 2.0 35.0 2.0 35.0 92-93 23.48079580283094 32.0 2.0 35.0 2.0 35.0 94-95 23.052151258046514 32.0 2.0 35.0 2.0 35.0 96-97 22.594666097653416 31.5 2.0 35.0 2.0 35.0 98-99 22.07574242799545 31.0 2.0 35.0 2.0 35.0 100-101 20.322357445767167 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3692623400518009 1101 2 0.20912856104544986 1101 3 0.6093485907201561 1101 4 0.18627386946372582 1101 5 0.43966289712303563 1101 6 0.22662428637450205 1101 7 0.07220674155270501 1101 8 -0.12457483789374635 1101 9 0.02460847426668522 1101 10-11 0.15202633382076414 1101 12-13 0.0390864515931284 1101 14-15 0.15946601491562973 1101 16-17 0.08508845750652227 1101 18-19 0.12705891734520236 1101 20-21 0.22303700219792688 1101 22-23 0.43918279986657893 1101 24-25 -0.02302370872225623 1101 26-27 0.2654586734923967 1101 28-29 0.40727862210592036 1101 30-31 0.24395198375925276 1101 32-33 0.3282944009476978 1101 34-35 0.2121241010620878 1101 36-37 -0.06081857444485905 1101 38-39 -0.04212316359885193 1101 40-41 0.3819890136283135 1101 42-43 0.25647735322854004 1101 44-45 0.040936603425002716 1101 46-47 -0.2837031162343209 1101 48-49 -0.4986195194906955 1101 50-51 -0.7033782764729821 1101 52-53 -0.8610663643943326 1101 54-55 -0.7458749831567246 1101 56-57 -0.5271233999882767 1101 58-59 -0.6422717266530178 1101 60-61 -0.537895027881067 1101 62-63 -0.4094118839914742 1101 64-65 -0.6080269174720598 1101 66-67 -0.5384061212145355 1101 68-69 -0.3446041801853674 1101 70-71 -0.3855883554486432 1101 72-73 -0.39132134133423335 1101 74-75 -0.8247857962877205 1101 76-77 -0.6491195491532196 1101 78-79 -0.5941952265214567 1101 80-81 -0.5737892471776007 1101 82-83 -0.2752396420966825 1101 84-85 -0.17873434588997839 1101 86-87 -0.17048441564308092 1101 88-89 -0.22920906408574382 1101 90-91 -0.06628893321778051 1101 92-93 -0.20779278250274658 1101 94-95 0.07673286419657543 1101 96-97 -0.3490738132047433 1101 98-99 -0.14428069847000202 1101 100-101 0.06220860014582996 1102 1 0.14942407215040632 1102 2 -0.09189458287583108 1102 3 -0.36692780305982353 1102 4 -0.22916458973369913 1102 5 -0.035983089283355696 1102 6 -0.2372396036012958 1102 7 0.08146720848892386 1102 8 -0.13380632058289876 1102 9 -0.013266690581218654 1102 10-11 0.13007076667151551 1102 12-13 0.2347264962288662 1102 14-15 0.0776108781824476 1102 16-17 0.2375528431129439 1102 18-19 -0.003562215069329966 1102 20-21 0.12953744420769198 1102 22-23 0.06765909799855052 1102 24-25 -0.045808949943655364 1102 26-27 -0.08926503417247744 1102 28-29 0.15688733477058747 1102 30-31 0.4341792202562118 1102 32-33 0.5558642167528021 1102 34-35 0.3929749336345836 1102 36-37 0.2117782691706367 1102 38-39 0.31919083724169894 1102 40-41 0.3846519413271139 1102 42-43 0.07953577097848807 1102 44-45 0.31124731455349774 1102 46-47 0.35288514804404514 1102 48-49 0.5893802776195756 1102 50-51 0.5412994911603093 1102 52-53 0.7295818682912056 1102 54-55 0.8540300934341474 1102 56-57 1.2522702495631926 1102 58-59 1.1905398902332003 1102 60-61 1.0731834759520993 1102 62-63 1.0239569550445111 1102 64-65 1.004576882362727 1102 66-67 1.2166081535271402 1102 68-69 0.8357140668473662 1102 70-71 0.6462435938868936 1102 72-73 0.6849108514689775 1102 74-75 0.5081417575867455 1102 76-77 0.8172152478845902 1102 78-79 1.0183433587574413 1102 80-81 0.9449997909801624 1102 82-83 0.9309361763459982 1102 84-85 0.9152941746091265 1102 86-87 1.4193993575262027 1102 88-89 1.2690953574611399 1102 90-91 1.0435831013314392 1102 92-93 0.6337068624402278 1102 94-95 0.5656934890969403 1102 96-97 0.7341145988932851 1102 98-99 0.8802762048530433 1102 100-101 1.1008953239114625 1103 1 0.0021106013075247176 1103 2 0.14021644335284478 1103 3 0.24549898583755336 1103 4 0.3153986901237005 1103 5 0.2708669234490131 1103 6 0.3996333273892887 1103 7 0.3368784639001774 1103 8 0.28687104127092766 1103 9 0.18930524728483533 1103 10-11 0.38229893066438336 1103 12-13 0.23402621611682406 1103 14-15 0.31373259959828914 1103 16-17 0.4177158173935638 1103 18-19 0.2736850474720498 1103 20-21 0.39901796368820897 1103 22-23 0.4699381914541121 1103 24-25 0.4384665784495212 1103 26-27 0.3060749199909978 1103 28-29 0.18725402690198223 1103 30-31 0.5359744838161831 1103 32-33 0.6569592002007312 1103 34-35 0.693267778045076 1103 36-37 0.6669603419997685 1103 38-39 0.9992901496939481 1103 40-41 0.9299155012965556 1103 42-43 1.0797904182919567 1103 44-45 1.3212422216072284 1103 46-47 1.2860402282579528 1103 48-49 1.1336252483351323 1103 50-51 1.0237288265308209 1103 52-53 1.4586371267582265 1103 54-55 1.5365485553643765 1103 56-57 1.4841266284783963 1103 58-59 1.3822893172767472 1103 60-61 1.3710571708846189 1103 62-63 1.4477537464883596 1103 64-65 1.223700895604388 1103 66-67 1.379518772320118 1103 68-69 1.1025844513648089 1103 70-71 1.0842369730567434 1103 72-73 1.085916708357189 1103 74-75 1.14794313268224 1103 76-77 1.1467288715158617 1103 78-79 1.5068842296512557 1103 80-81 1.4484375297120167 1103 82-83 1.303294210468735 1103 84-85 1.1798090714769671 1103 86-87 1.5298526297441768 1103 88-89 1.516230575184597 1103 90-91 1.4510188028847892 1103 92-93 1.6489857012484812 1103 94-95 1.4473461501613656 1103 96-97 1.3417667506630835 1103 98-99 1.642435614071271 1103 100-101 1.8260035490196884 1104 1 0.3696127745906317 1104 2 0.4144364761935613 1104 3 0.49996629000059656 1104 4 0.2499828686226877 1104 5 0.5216879415114093 1104 6 0.39282370698940383 1104 7 0.48845288677277665 1104 8 0.36766887764686373 1104 9 0.412235798815793 1104 10-11 0.4594625547346993 1104 12-13 0.649111215633873 1104 14-15 0.8085408677331216 1104 16-17 0.5845394433956912 1104 18-19 0.5882074081404554 1104 20-21 0.5853045472871656 1104 22-23 0.5371409346203464 1104 24-25 0.7792494433196069 1104 26-27 0.5542517769406814 1104 28-29 0.7532976857448404 1104 30-31 0.9528343312034124 1104 32-33 1.1985051289170414 1104 34-35 1.031720400473354 1104 36-37 1.0449425693734753 1104 38-39 1.0979572027220499 1104 40-41 0.9806012928545584 1104 42-43 1.0545803342583397 1104 44-45 1.3857525084806 1104 46-47 1.5638461782241464 1104 48-49 1.353018177920756 1104 50-51 1.2743808114627235 1104 52-53 1.2901110956561297 1104 54-55 1.639381067679647 1104 56-57 1.1367514715190588 1104 58-59 1.216117676206526 1104 60-61 1.3072713117133645 1104 62-63 1.4893358965897825 1104 64-65 1.8535304136184934 1104 66-67 1.6389604800376851 1104 68-69 1.5613041461392712 1104 70-71 1.740556397378196 1104 72-73 1.6823984044839122 1104 74-75 1.6891147737524612 1104 76-77 1.6183554531830104 1104 78-79 1.8871966994667702 1104 80-81 1.8166882782873088 1104 82-83 1.5018839912086186 1104 84-85 1.3341966885927796 1104 86-87 1.2631666450054553 1104 88-89 1.1629271848629514 1104 90-91 1.036622009684752 1104 92-93 1.23695537187535 1104 94-95 1.4676542734106626 1104 96-97 1.3297412508079667 1104 98-99 1.8675756700936823 1104 100-101 1.5835617447117585 1105 1 0.12242734970776326 1105 2 0.18057393532323474 1105 3 0.32170424800107256 1105 4 0.12144779598305178 1105 5 0.04785337785482113 1105 6 0.03048508158070007 1105 7 0.3238339499068985 1105 8 0.20015952381937296 1105 9 0.31060772722108965 1105 10-11 0.3763188260125503 1105 12-13 0.4600585645400699 1105 14-15 0.7528308195314182 1105 16-17 0.6747617442687144 1105 18-19 0.6372433740284791 1105 20-21 0.624577982417108 1105 22-23 0.6198843869411022 1105 24-25 0.4136959663923889 1105 26-27 0.6593980674317947 1105 28-29 0.6234648748775413 1105 30-31 0.5814536725591282 1105 32-33 0.7865870044015892 1105 34-35 0.3356608056373389 1105 36-37 0.6754663837126387 1105 38-39 0.8806450376902077 1105 40-41 1.2468651550583516 1105 42-43 1.166994801511521 1105 44-45 1.21296371959059 1105 46-47 1.8979084359515852 1105 48-49 2.164117210515908 1105 50-51 1.8313633172772654 1105 52-53 2.143761616436791 1105 54-55 2.574532077218862 1105 56-57 2.745715134848684 1105 58-59 1.976377392389491 1105 60-61 1.9244427479893602 1105 62-63 2.1883954577183076 1105 64-65 2.1475818849502737 1105 66-67 2.2967789315157567 1105 68-69 2.1681273840804174 1105 70-71 2.328579284761485 1105 72-73 2.1689111964750154 1105 74-75 2.172623386015246 1105 76-77 2.260289251679655 1105 78-79 2.230435820375522 1105 80-81 2.251278900864584 1105 82-83 2.1267702284419094 1105 84-85 2.2645696619301567 1105 86-87 2.3443174185922686 1105 88-89 2.156278383164093 1105 90-91 2.1934882246711 1105 92-93 2.1202516269736087 1105 94-95 1.8647705979463431 1105 96-97 1.9646217577685654 1105 98-99 1.609877722879606 1105 100-101 1.4627204058503978 1106 1 0.0698043454718551 1106 2 0.07797751269285769 1106 3 -0.07051162893467122 1106 4 0.0611847238354315 1106 5 -0.012341096601112156 1106 6 0.2155702284655021 1106 7 0.10173177364512753 1106 8 0.10713247917942681 1106 9 -0.12365853471919053 1106 10-11 0.28752768883431656 1106 12-13 0.26886822309505476 1106 14-15 0.22061991592832442 1106 16-17 0.3502432143172456 1106 18-19 0.34753388525277984 1106 20-21 0.04681392417766972 1106 22-23 -0.1081000413828832 1106 24-25 0.17380983856058663 1106 26-27 0.057856334311118474 1106 28-29 0.4142676393645104 1106 30-31 0.3910779287029058 1106 32-33 0.07683241264360419 1106 34-35 0.3169593599409879 1106 36-37 -0.07846940884793696 1106 38-39 0.13884246184688465 1106 40-41 0.2800064148549595 1106 42-43 0.04434641612969159 1106 44-45 0.25082107069072634 1106 46-47 0.26350840851250723 1106 48-49 0.40481793593648874 1106 50-51 -0.2661011405437286 1106 52-53 -0.09958440746950004 1106 54-55 -0.0580629734576803 1106 56-57 0.06947480283585605 1106 58-59 0.07513663164109019 1106 60-61 0.15469518749477018 1106 62-63 0.4130100244381403 1106 64-65 0.43608233940998176 1106 66-67 0.4895856060711594 1106 68-69 0.5744872475710103 1106 70-71 0.0018742033874730168 1106 72-73 -0.004357998016587317 1106 74-75 0.2007098534056162 1106 76-77 0.5289220996843227 1106 78-79 0.27222896403623764 1106 80-81 0.4959582924030599 1106 82-83 0.7867700569476774 1106 84-85 0.6502130225310729 1106 86-87 0.6209504723900103 1106 88-89 0.7328505565104599 1106 90-91 0.6790398358593812 1106 92-93 0.4273163317462938 1106 94-95 0.7775957938785005 1106 96-97 0.6006123941836492 1106 98-99 0.5437452607369586 1106 100-101 0.8290749530198873 1107 1 0.13131228197979183 1107 2 0.3723593004956989 1107 3 0.3267314788347555 1107 4 0.3299295024486284 1107 5 0.4196764472585386 1107 6 0.3444862685657526 1107 7 0.3738182398105394 1107 8 0.4771721617191105 1107 9 0.46118900078623426 1107 10-11 0.6124284824851074 1107 12-13 0.6027236951084234 1107 14-15 0.3497970253685949 1107 16-17 0.41561497705743733 1107 18-19 0.4940982127381588 1107 20-21 0.7614129216714076 1107 22-23 0.6879003763280664 1107 24-25 0.455973581250646 1107 26-27 0.3383753401590681 1107 28-29 0.4500341389468048 1107 30-31 0.471461178494053 1107 32-33 0.6576594803127804 1107 34-35 0.6391711393896102 1107 36-37 0.4127586613275014 1107 38-39 0.5413069564166406 1107 40-41 0.9189736245458562 1107 42-43 0.9920803342583469 1107 44-45 0.4324992244083532 1107 46-47 0.15482524226354855 1107 48-49 0.180193875786113 1107 50-51 -0.12201697178850779 1107 52-53 -0.2978404642781882 1107 54-55 -0.4470823970165796 1107 56-57 -0.4957403183003173 1107 58-59 -0.24498659308740045 1107 60-61 -0.3351753221125797 1107 62-63 -0.31931558124272996 1107 64-65 0.02455873874164638 1107 66-67 -0.229112180060838 1107 68-69 -0.6112635878508748 1107 70-71 -0.6054514022933972 1107 72-73 -0.5311176053683013 1107 74-75 -0.4212095283821853 1107 76-77 -0.27501482596312954 1107 78-79 -0.17124952385014325 1107 80-81 -0.007357288215153801 1107 82-83 -0.2842408035368642 1107 84-85 -0.07903196493759879 1107 86-87 -0.09926151591408328 1107 88-89 0.2025926200025232 1107 90-91 0.5631511558259668 1107 92-93 0.8417378020888151 1107 94-95 0.5509876067439983 1107 96-97 0.39455036410846134 1107 98-99 0.5491232891413027 1107 100-101 0.14288029971996608 1108 1 0.24927920938476333 1108 2 0.30009019112284463 1108 3 -0.03802768131345502 1108 4 0.3529756805740618 1108 5 0.2682595404381729 1108 6 0.17398571964301368 1108 7 0.11128736290646657 1108 8 0.08231659701484517 1108 9 0.0797305816837266 1108 10-11 0.11349630875105987 1108 12-13 0.1507715886174097 1108 14-15 0.5911686460629824 1108 16-17 0.4603178726248771 1108 18-19 0.14881723291106397 1108 20-21 0.35491847951414 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-1.9979307121847931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 699.0 3 226.0 4 199.0 5 217.0 6 296.0 7 369.0 8 528.0 9 682.0 10 969.0 11 1633.0 12 2758.0 13 3863.0 14 4499.0 15 6652.0 16 9321.0 17 13557.0 18 10303.0 19 4574.0 20 3233.0 21 2938.0 22 2926.0 23 3098.0 24 3472.0 25 3682.0 26 4254.0 27 4917.0 28 5648.0 29 6652.0 30 8157.0 31 9869.0 32 12364.0 33 16006.0 34 22218.0 35 32720.0 36 42184.0 37 47050.0 38 36699.0 39 5650.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.372417417058784 18.531600066878447 15.368607734014855 16.727374782047914 2 17.34554650249747 22.56858711586289 37.839128916011575 22.24673746562807 3 21.381982525216646 28.82622829458174 32.44566369251068 17.346125487690934 4 11.980592670000746 18.158393670224676 38.09509591699634 31.76591774277823 5 11.215201667572524 39.615299619540224 31.198298980463363 17.97119973242389 6 25.14499202618517 37.13737061216201 17.754131750117967 19.963505611534853 7 23.220839567226 38.97952296909482 20.05118511152322 17.748452352155955 8 27.244709634646743 27.719842080429082 19.53627551111828 25.499172773805896 9 29.05508918732474 15.902609173468077 22.31161361416963 32.73068802503755 10-11 25.54111907680867 26.616114933019947 24.157914935110508 23.684851055060875 12-13 31.009242806587928 26.133998297770678 25.317824132833618 17.53893476280778 14-15 25.67538310157362 25.596993419746138 31.01618410973958 17.711439368940667 16-17 19.153599352643546 28.87529281084979 29.865288346837342 22.105819489669322 18-19 18.344908098553702 25.44398132113554 37.74378665010569 18.467323930205062 20-21 22.01635707268715 21.868402901155864 37.294603479834336 18.820636546322646 22-23 20.66491330722201 22.95418179212839 37.716433416125334 18.664471484524263 24-25 20.58111994460123 23.173609173033096 38.12888869653205 18.116382185833615 26-27 19.722030286252394 22.591568381355465 40.19606818958532 17.49033314280683 28-29 18.411273255866014 24.97828507296514 38.44601713912179 18.164424532047054 30-31 18.827137463250107 23.77244168519707 38.18660736937932 19.213813482173503 32-33 18.093310889056028 24.217882597328174 38.4301541253298 19.258652388285995 34-35 19.827308325971206 23.800171915665818 37.928296841049644 18.44422291731334 36-37 19.404107616385698 23.13044957552442 38.46156141950484 19.003881388585047 38-39 19.318339528935276 24.529062123656356 37.42760398090431 18.724994366504056 40-41 18.83520094552064 25.53636956486054 36.48954569941815 19.13888379020067 42-43 18.35295416797862 25.671483209748686 34.79103027662169 21.184532345651007 44-45 19.55923892688153 24.661013281922646 34.389749298300856 21.38999849289497 46-47 20.941816184984898 26.122282706012967 32.887762776205996 20.048138332796142 48-49 20.551150318878122 26.23768581328097 31.194696207782595 22.016467660058314 50-51 19.679819973348174 27.39482154768602 31.27082640052647 21.65453207843933 52-53 21.232501607889777 28.44390591121464 27.5243869500532 22.799205530842386 54-55 20.658451198147194 26.186880519550286 28.728820894230566 24.425847388071954 56-57 22.667327359239646 26.63357233688157 26.675363132160406 24.02373717171838 58-59 23.506096787527962 26.13672144896263 27.10328350547002 23.25389825803939 60-61 22.986592957534167 26.607374992724107 28.88133357014183 21.524698479599895 62-63 23.546697310688593 26.777602005790524 28.25970808584306 21.415992597677818 64-65 22.93993782915404 28.542327959420316 27.8879372740039 20.629796937421744 66-67 23.62216945722528 27.120528552048555 26.630868717138988 22.626433273587175 68-69 23.33941317125739 26.802234116547808 27.946159104681673 21.912193607513128 70-71 23.932828091373377 27.223517331749353 27.501261114974884 21.342393461902386 72-73 22.559838103567152 26.49740768709574 29.41700569204457 21.525748517292534 74-75 20.702557168399405 29.42206262928371 28.375256328745536 21.50012387357135 76-77 20.34041486195646 28.07945003477581 28.69436990116506 22.885765202102668 78-79 20.1903829303208 27.459087971124607 29.767298141698994 22.58323095685559 80-81 20.57184652242382 27.632232977826206 28.745326039093342 23.050594460656633 82-83 19.810789873168314 27.17514773644922 29.235770804381865 23.7782915860006 84-85 20.053072884173954 27.21403177806102 28.09788335308393 24.6350119846811 86-87 19.53575911533932 28.06072265995221 27.697586705612114 24.705931519096357 88-89 19.565275791974734 27.033105782691624 27.14921929334929 26.25239913198435 90-91 19.482721309762777 26.25883336193838 28.08109903066212 26.177346297636728 92-93 20.462178226097755 26.66107427629792 29.054209368348467 23.822538129255864 94-95 19.801791044776117 26.596268656716415 29.194179104477612 24.40776119402985 96-97 18.18466797530993 27.34671333995119 30.03799046133407 24.43062822340481 98-99 19.536545455631266 27.308613368846636 28.037342713303754 25.117498462218347 100-101 19.981575132102122 26.194070298187228 29.60595568177237 24.21839888793828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1765.0 1 1165.0 2 561.5 3 526.0 4 456.5 5 456.5 6 681.0 7 1305.5 8 1987.0 9 2126.0 10 1624.0 11 1137.0 12 1017.0 13 1014.0 14 1079.5 15 1096.5 16 1086.5 17 1154.5 18 1244.0 19 1319.0 20 1427.5 21 1586.5 22 1752.5 23 1930.0 24 2109.0 25 2424.0 26 2989.0 27 3677.5 28 4060.5 29 4275.5 30 4622.5 31 5010.0 32 5603.5 33 6311.0 34 7140.0 35 7649.5 36 7780.5 37 8180.0 38 7674.5 39 6620.5 40 6298.0 41 6548.5 42 7091.5 43 7672.5 44 8168.0 45 8200.5 46 8704.5 47 13281.0 48 22676.5 49 22097.5 50 12805.0 51 8211.5 52 6570.0 53 6185.5 54 5984.5 55 5879.5 56 6154.5 57 6456.5 58 6854.5 59 7363.0 60 8157.5 61 8841.5 62 7664.0 63 4449.0 64 2090.0 65 1450.0 66 1033.5 67 735.0 68 536.5 69 386.5 70 296.0 71 286.5 72 290.5 73 279.5 74 214.0 75 173.5 76 129.5 77 50.0 78 23.5 79 15.0 80 9.0 81 5.0 82 3.0 83 3.0 84 3.0 85 2.5 86 2.5 87 1.5 88 1.0 89 0.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04386972779878418 2 0.04297442723146205 3 0.025665282929900952 4 0.04715249654563198 5 0.06714754254915946 6 0.07072874481844797 7 0.06565537493695592 8 0.0683412766389223 9 0.06744597607160016 10-11 0.07296699623675329 12-13 0.06804284311648158 14-15 0.06490929113085414 16-17 0.054464117845429344 18-19 0.04745093006807269 20-21 0.054314901084208986 22-23 0.03193238690115584 24-25 0.017159927540340753 26-27 0.013877158793492954 28-29 0.017906011346442523 30-31 0.01432480907715402 32-33 0.00925143919566197 34-35 0.010445173285424806 36-37 0.006714754254915946 38-39 0.009997523001763741 40-41 0.008803788912000907 42-43 0.006714754254915946 44-45 0.0010445173285424806 46-47 0.002089034657084961 48-49 5.968670448814174E-4 50-51 0.006416320732475238 52-53 0.004924153120271693 54-55 0.00716240453857701 56-57 0.024173115317697405 58-59 0.008803788912000907 60-61 0.021636430376951385 62-63 0.015816976689357564 64-65 0.011489690613967285 66-67 0.016264626973018625 68-69 0.02864961815430804 70-71 0.01850287839132394 72-73 0.01611541021179827 74-75 0.019099745436205358 76-77 0.023874681795256696 78-79 0.037751840588749655 80-81 0.03446907184190186 82-83 0.03327533775213902 84-85 0.021487213615731027 86-87 0.025217632646239888 88-89 0.019696612481086773 90-91 0.018055228107662878 92-93 0.031037086333833706 94-95 0.02476998236257882 96-97 0.038945574678512486 98-99 0.05521020165153111 100-101 0.06252182295132848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 335083.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.1026446971016 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62827533935133 32.31830064419234 2 11.727789405425568 9.640863220272793 3 3.8930033037176375 4.800381947920463 4 1.7430923391704003 2.8658282036464273 5 0.8850874769152919 1.818971804475168 6 0.5742141616690001 1.4161032400274978 7 0.3766655108274034 1.0837364062833728 8 0.25443225305524064 0.8366270797450078 9 0.1904431124110209 0.7044944025996336 >10 1.4050029239290414 11.234667549688561 >50 0.16063366218893746 4.691636328683611 >100 0.13446709278259472 11.221507395675314 >500 0.012356435552995192 3.508868357131241 >1k 0.013083284703171378 10.236514720199647 >5k 0.0014536983003523754 3.621498699458911 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6557 1.956828606643727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5565 1.6607825523825441 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4514 1.347128920297359 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3713 1.1080836688223514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3284 0.9800556876952875 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2608 0.7783146265253683 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2134 0.6368571368884723 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.6129824550932157 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1759 0.5249445659732066 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1489 0.4443675149142153 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1439 0.4294458387921798 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1428 0.426163070045332 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1350 0.4028852552949568 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.4013930876827532 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1341 0.4001993535929904 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1336 0.3987071859807868 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1258 0.37542937123041153 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1122 0.3348424121784752 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1068 0.3187270019666769 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1022 0.3049990599344043 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 864 0.2578465633887723 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 825 0.2462076560135847 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 814 0.24292488726673692 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 809 0.24143271965453333 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 807 0.24083585260965193 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 803 0.23964211851988912 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 757 0.22591417648761652 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 733 0.21875177194903947 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 732 0.21845333842659878 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.21009719979825894 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 624 0.18622251800300224 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 600 0.17906011346442524 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 588 0.17547891119513673 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 545 0.16264626973018625 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 525 0.1566775992813721 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 511 0.15249952996720215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 504 0.15041049531011721 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 496 0.1480230271305915 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 479 0.1429496572490995 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 477 0.14235279020421804 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 448 0.1336982180534375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 445 0.1328029174861154 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 441 0.13160918339635255 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 441 0.13160918339635255 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 433 0.12922171521682688 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 0.12772954760462335 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 416 0.12414834533533482 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 410 0.12235774420069057 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 409 0.12205931067824988 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 401 0.1196718424987242 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.1190749754538428 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 396 0.11817967488652065 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 389 0.11609064022943569 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 381 0.11370317204991003 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 380 0.11340473852746932 No Hit GAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCC 378 0.1128078714825879 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 372 0.11101727034794363 No Hit GTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAG 370 0.11042040330306221 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 362 0.10803293512353655 No Hit CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC 343 0.1023626981971631 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.984335224407087E-4 0.0 16-17 0.0 0.0 0.0 4.476502836610631E-4 0.0 18-19 0.0 0.0 0.0 5.968670448814174E-4 0.0 20-21 0.0 0.0 0.0 5.968670448814174E-4 0.0 22-23 0.0 0.0 0.0 0.002089034657084961 0.0 24-25 0.0 0.0 0.0 0.0035812022692885046 0.0 26-27 0.0 0.0 0.0 0.007013187777356655 0.0 28-29 0.0 0.0 0.0 0.018204444868883233 0.0 30-31 0.0 0.0 0.0 0.038199490872410716 0.0 32-33 0.0 0.0 0.0 0.05938827096570103 0.0 34-35 0.0 0.0 0.0 0.08027861753655065 0.0 36-37 0.0 0.0 0.0 0.09758776183811176 0.0 38-39 0.0 0.0 0.0 0.11415082233357109 0.0 40-41 0.0 0.0 0.0 0.1268342470373012 0.0 42-43 0.0 0.0 0.0 0.14548634218984552 0.0 44-45 0.0 0.0 0.0 0.1601095847894402 0.0 46-47 0.0 0.0 0.0 0.1880131191376465 0.0 48-49 0.0 0.0 0.0 0.21606587024707313 0.0 50-51 0.0 0.0 0.0 0.2393436849974484 0.0 52-53 0.0 0.0 0.0 0.2781400429147405 0.0 54-55 0.0 0.0 0.0 0.36737166612451244 0.0 56-57 0.0 0.0 0.0 0.5412091929462253 0.0 58-59 0.0 0.0 0.0 0.7072874481844796 0.0 60-61 0.0 0.0 0.0 0.8432239176562224 0.0 62-63 0.0 0.0 0.0 1.0154200601045114 0.0 64-65 0.0 0.0 0.0 1.212236968154159 0.0 66-67 0.0 0.0 0.0 1.5245476493883605 0.0 68-69 0.0 0.0 0.0 1.9023644887982978 0.0 70-71 0.0 0.0 0.0 2.2345209992748067 0.0 72-73 0.0 0.0 0.0 2.5857772551875207 0.0 74-75 0.0 0.0 0.0 2.9604605426118304 0.0 76-77 0.0 0.0 0.0 3.3440968357093617 0.0 78-79 0.0 0.0 0.0 3.6704338924982762 0.0 80-81 0.0 0.0 0.0 4.003485703542108 0.0 82-83 0.0 0.0 0.0 4.37533387250323 0.0 84-85 0.0 0.0 0.0 4.959815926203358 0.0 86-87 0.0 0.0 0.0 5.665611206775635 0.0 88-89 0.0 0.0 0.0 6.437509512568528 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 90 0.0 63.39103 1 ACGAATC 20 5.350581E-4 47.564606 10-11 GTCACTA 160 0.0 38.62891 2 CATGGGT 285 0.0 36.700073 4 GATTCGA 65 1.3156088E-4 36.588158 9 ATGGGTT 85 1.6186403E-5 33.55996 5 GGGATTC 175 3.6379788E-12 32.605976 7 TAAAACG 60 0.003929429 31.695515 4 CGCAAAG 30 0.0039466247 31.671846 88-89 CCTAGCT 65 0.005812307 29.2574 2 TAAGGTG 65 0.005812307 29.2574 5 ATGGGTA 135 4.176636E-7 28.17379 5 TCACTAC 225 3.6379788E-12 27.469448 3 GGTGTGC 105 6.827974E-5 27.179771 8 GTACATG 4200 0.0 27.167585 1 TACATGG 4200 0.0 26.827991 2 GGGATTG 125 7.3099836E-6 26.628214 7 TATCAAA 90 8.886866E-4 26.412928 2 ACTAATC 45 8.919523E-4 26.40109 18-19 GGGAACT 255 0.0 26.106092 7 >>END_MODULE