##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937030_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 335083 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97927976053694 31.0 31.0 34.0 30.0 34.0 2 31.973654288638933 31.0 31.0 34.0 30.0 34.0 3 32.044523297212926 33.0 31.0 34.0 30.0 34.0 4 35.823921237424756 37.0 35.0 37.0 35.0 37.0 5 35.76502836610631 37.0 35.0 37.0 35.0 37.0 6 35.88279620273186 37.0 35.0 37.0 35.0 37.0 7 35.83854448002435 37.0 35.0 37.0 35.0 37.0 8 35.86092997854263 37.0 35.0 37.0 35.0 37.0 9 37.43134984466535 39.0 37.0 39.0 34.0 39.0 10-11 37.33738805012489 39.0 37.0 39.0 34.0 39.0 12-13 37.262039554379065 39.0 37.0 39.0 34.0 39.0 14-15 38.12679992718222 40.0 37.0 41.0 33.0 41.0 16-17 38.13270145008849 40.0 37.5 41.0 33.5 41.0 18-19 38.33997696093206 40.0 38.0 41.0 33.5 41.0 20-21 38.47829492991289 40.0 38.0 41.0 34.0 41.0 22-23 38.375847178161834 40.0 38.0 41.0 34.0 41.0 24-25 38.2104075706616 40.0 37.0 41.0 33.5 41.0 26-27 37.90731251660036 40.0 36.5 41.0 33.0 41.0 28-29 37.6792287284046 39.5 36.0 41.0 33.0 41.0 30-31 37.28992965921876 39.0 35.5 41.0 33.0 41.0 32-33 36.80252206169814 38.5 35.0 41.0 32.5 41.0 34-35 36.418600764586685 38.0 35.0 41.0 31.0 41.0 36-37 36.009294712056416 38.0 35.0 40.5 30.5 41.0 38-39 35.65276065929934 38.0 35.0 40.0 30.0 41.0 40-41 35.209601800151006 38.0 35.0 40.0 29.0 41.0 42-43 34.57331765562562 38.0 35.0 40.0 25.0 41.0 44-45 33.70686814908545 38.0 33.5 40.0 16.0 41.0 46-47 32.46366422647523 37.5 33.0 40.0 2.0 41.0 48-49 31.348183882799187 37.0 31.5 40.0 2.0 41.0 50-51 30.75497264856767 37.0 31.0 40.0 2.0 41.0 52-53 30.39778204206122 36.0 30.0 40.0 2.0 41.0 54-55 30.05297493456845 35.5 29.0 40.0 2.0 41.0 56-57 29.688193671418723 35.0 28.5 39.5 2.0 41.0 58-59 29.317100539269376 35.0 27.5 39.0 2.0 41.0 60-61 28.86507969667217 35.0 26.0 39.0 2.0 40.0 62-63 28.538650125491294 35.0 26.0 38.0 2.0 40.0 64-65 28.125528600376622 35.0 25.5 38.0 2.0 40.0 66-67 27.663431149894205 34.0 24.5 37.0 2.0 39.5 68-69 27.210574693434165 34.0 22.5 36.5 2.0 39.0 70-71 26.804543948812682 34.0 22.0 36.0 2.0 39.0 72-73 26.473894527624502 34.0 21.0 35.5 2.0 38.0 74-75 26.082913785539702 33.0 19.5 35.0 2.0 37.0 76-77 24.784277328303734 31.5 17.5 34.0 2.0 36.0 78-79 25.426491346920017 33.0 17.5 35.0 2.0 36.0 80-81 25.13141072510393 33.0 9.0 35.0 2.0 36.0 82-83 24.914537592178654 33.0 7.5 35.0 2.0 35.5 84-85 24.62207423235437 33.0 2.0 35.0 2.0 35.0 86-87 24.30744919915364 32.5 2.0 35.0 2.0 35.0 88-89 23.925272245980846 32.0 2.0 35.0 2.0 35.0 90-91 23.434238681162576 32.0 2.0 35.0 2.0 35.0 92-93 23.04644670126506 31.0 2.0 34.0 2.0 35.0 94-95 22.543128120495517 31.0 2.0 34.0 2.0 35.0 96-97 21.924761924657474 31.0 2.0 34.0 2.0 35.0 98-99 21.302026662050896 30.5 2.0 34.0 2.0 35.0 100-101 18.812373352273916 25.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19125740056188434 1101 2 0.05062358630740604 1101 3 0.07623791600791208 1101 4 0.16409636036055275 1101 5 0.18347601880067543 1101 6 0.07356546855781687 1101 7 0.1856804424104297 1101 8 0.14482142367486261 1101 9 0.24352256880210632 1101 10-11 0.15357547283783646 1101 12-13 0.22135505611078088 1101 14-15 0.23771487316846418 1101 16-17 0.29964791494546006 1101 18-19 0.2960479923996431 1101 20-21 0.03347349267726685 1101 22-23 0.23247065001784506 1101 24-25 0.4165551845458282 1101 26-27 0.5358218867387308 1101 28-29 0.26677701397220943 1101 30-31 -0.00990797230122098 1101 32-33 -0.038229709719580285 1101 34-35 0.04130449082051513 1101 36-37 0.33640044194803664 1101 38-39 0.22448489139395633 1101 40-41 0.33391498770928507 1101 42-43 -0.018923584936395343 1101 44-45 -0.04807465253605869 1101 46-47 -0.1669077523186857 1101 48-49 -0.3501385447125749 1101 50-51 0.004443704622435263 1101 52-53 -0.0714237180816717 1101 54-55 -0.016010285486235176 1101 56-57 -0.15661075418564963 1101 58-59 -0.2874093671935931 1101 60-61 -0.3684855925866337 1101 62-63 -0.3906816778672315 1101 64-65 -0.4345861645931528 1101 66-67 -0.49802019318372004 1101 68-69 -0.4837238650537792 1101 70-71 -0.37874846471374823 1101 72-73 -0.39934703069178923 1101 74-75 -0.625352654392362 1101 76-77 -0.6816595138019252 1101 78-79 -0.45051242747435083 1101 80-81 -0.3239528207022282 1101 82-83 -0.8943490518384714 1101 84-85 -0.7918901694796396 1101 86-87 -0.734163868481005 1101 88-89 -0.7181260933235727 1101 90-91 -0.5959772526399938 1101 92-93 -1.0199131476610077 1101 94-95 -0.6289555144533807 1101 96-97 -0.45263890476154955 1101 98-99 -0.4045577698898377 1101 100-101 -0.5686031609054645 1102 1 0.21835042550283035 1102 2 0.32848107076460664 1102 3 0.14698121702380718 1102 4 0.3514070569969334 1102 5 0.19478712121651398 1102 6 0.25860090179984496 1102 7 0.1641089165347438 1102 8 0.12343104934330995 1102 9 -0.003321771299049203 1102 10-11 0.17382821547221283 1102 12-13 0.18014670285077727 1102 14-15 0.1941443037729229 1102 16-17 0.23898388584527908 1102 18-19 0.23400358853314884 1102 20-21 0.341753567456621 1102 22-23 0.15340887009523385 1102 24-25 0.2463125863979272 1102 26-27 -0.13446781081065495 1102 28-29 0.06546692600500847 1102 30-31 0.32338550769240015 1102 32-33 0.2793832926136517 1102 34-35 0.4976578377764511 1102 36-37 0.4174331506708455 1102 38-39 0.2725262808987594 1102 40-41 0.10479813771726043 1102 42-43 -0.09507607106018412 1102 44-45 0.5885896953908372 1102 46-47 0.43755872015352537 1102 48-49 1.0981571815601932 1102 50-51 0.9074732830465599 1102 52-53 0.95349548832964 1102 54-55 1.180781882970205 1102 56-57 1.3413096260876713 1102 58-59 1.437819970661284 1102 60-61 1.2632514333397786 1102 62-63 1.4901640824620301 1102 64-65 1.2133675475642924 1102 66-67 1.1988951583227347 1102 68-69 1.2737649397807935 1102 70-71 1.3440824266967688 1102 72-73 1.2757286906536613 1102 74-75 1.644827627624359 1102 76-77 1.9370853233790655 1102 78-79 1.3921139800731481 1102 80-81 1.5245320277826195 1102 82-83 1.4742726022925083 1102 84-85 1.2808675367070457 1102 86-87 1.2570720198137053 1102 88-89 1.167002089509058 1102 90-91 1.1242378104732005 1102 92-93 1.4275311663543206 1102 94-95 1.4174047887218428 1102 96-97 1.7329907055452445 1102 98-99 2.2154225208221625 1102 100-101 2.27021906421518 1103 1 0.19708737482801197 1103 2 -0.10569259870318248 1103 3 -0.10219117920741283 1103 4 0.15252750517620228 1103 5 0.3415912974324513 1103 6 0.13102259775095604 1103 7 0.29257848618078697 1103 8 0.21765055532752342 1103 9 -0.011470485330114855 1103 10-11 0.06623972553078517 1103 12-13 -0.04534349322764797 1103 14-15 0.3506250779007587 1103 16-17 0.21211859427410928 1103 18-19 0.3568072554544628 1103 20-21 0.16649255396031037 1103 22-23 0.13545623449554256 1103 24-25 0.35383741451098416 1103 26-27 0.37126175374243786 1103 28-29 -0.10438283208004151 1103 30-31 0.31288130601173236 1103 32-33 -0.020272933513162172 1103 34-35 -0.1167424678003286 1103 36-37 0.2553501356466512 1103 38-39 0.26475953783790374 1103 40-41 0.015862563487566206 1103 42-43 -0.12913514923691594 1103 44-45 0.19458663962307554 1103 46-47 0.12281464070355952 1103 48-49 0.08434256480440183 1103 50-51 0.25105617074709485 1103 52-53 0.3152219971149748 1103 54-55 0.18835764054596282 1103 56-57 0.09035469866215351 1103 58-59 -0.08675349508865438 1103 60-61 -0.2955084342436187 1103 62-63 0.00442433201639858 1103 64-65 -0.017788551238869132 1103 66-67 0.12396518633394038 1103 68-69 0.0033294928747586994 1103 70-71 0.09936635845128805 1103 72-73 0.3716670660037984 1103 74-75 0.22446857491236827 1103 76-77 0.29989408324665234 1103 78-79 0.7712201680028663 1103 80-81 0.8861312129112164 1103 82-83 0.6093630020887915 1103 84-85 0.4820389143490118 1103 86-87 0.732689539548872 1103 88-89 0.7827392934815549 1103 90-91 0.5522999444176868 1103 92-93 0.5119467507699085 1103 94-95 0.7231088885435888 1103 96-97 0.8517836772793927 1103 98-99 1.2764105523984277 1103 100-101 1.4652152445054796 1104 1 0.11839037878821657 1104 2 0.2010300903724449 1104 3 0.18335407337637832 1104 4 0.16056865266968146 1104 5 -0.0376948998050608 1104 6 0.1429515072457903 1104 7 -0.06924900343575047 1104 8 0.19654556160589465 1104 9 0.24048546946559668 1104 10-11 0.17853809315369773 1104 12-13 0.16138402536599017 1104 14-15 0.2162799605434671 1104 16-17 0.021461296872388402 1104 18-19 0.20397715789820126 1104 20-21 0.3735702126099767 1104 22-23 0.276695488817964 1104 24-25 0.338443325841034 1104 26-27 0.3970030847576069 1104 28-29 0.301598525980431 1104 30-31 0.2692949059344585 1104 32-33 0.3154872326222531 1104 34-35 0.72739691208956 1104 36-37 0.6528194682072623 1104 38-39 0.7518888723303121 1104 40-41 0.4396527692250274 1104 42-43 0.28573252196949994 1104 44-45 0.47700477929274143 1104 46-47 0.9767820897521489 1104 48-49 0.9884162632492952 1104 50-51 0.8516308833907722 1104 52-53 0.9071880938985153 1104 54-55 0.898296788618989 1104 56-57 0.6973164488794836 1104 58-59 0.8637680928568692 1104 60-61 0.971938199582322 1104 62-63 1.1508915395890824 1104 64-65 0.9702825392663001 1104 66-67 1.0625699385796636 1104 68-69 0.9876456331239574 1104 70-71 1.359979224287276 1104 72-73 1.0940180753730644 1104 74-75 1.216028992182725 1104 76-77 0.8743048820489321 1104 78-79 0.7905865352394166 1104 80-81 0.969150627573633 1104 82-83 0.8386081484231873 1104 84-85 0.7641793567328641 1104 86-87 1.1283840242393026 1104 88-89 0.9400366952008632 1104 90-91 1.4927761348938802 1104 92-93 1.3282318855134605 1104 94-95 1.157185967651099 1104 96-97 1.3614789346927054 1104 98-99 1.3452553316894544 1104 100-101 1.1508739907626016 1105 1 0.2905429388718126 1105 2 0.23084436212220183 1105 3 0.31681043903519424 1105 4 0.22478984119565126 1105 5 0.17969310707126596 1105 6 0.20512496597097396 1105 7 0.1369396910732945 1105 8 0.23279796326430358 1105 9 0.3625571315817524 1105 10-11 0.16848062188933 1105 12-13 0.3899966252244198 1105 14-15 0.5399266049631919 1105 16-17 0.3810401218294075 1105 18-19 0.5774139732148811 1105 20-21 0.6937028515690926 1105 22-23 0.20809232705573777 1105 24-25 0.49501881375690004 1105 26-27 0.5614956185493583 1105 28-29 0.36010208212024253 1105 30-31 0.3245499030693253 1105 32-33 0.235351897064227 1105 34-35 -0.012907972125219658 1105 36-37 0.20585819810917627 1105 38-39 0.1698869847110771 1105 40-41 0.39421507751369944 1105 42-43 0.16492910403037797 1105 44-45 0.009022677968033577 1105 46-47 0.35950110414524517 1105 48-49 0.49469849603319815 1105 50-51 0.4709975197207008 1105 52-53 0.36819274553164405 1105 54-55 0.10052485347219076 1105 56-57 0.22918266386582786 1105 58-59 0.3269005295710592 1105 60-61 0.48280465052107857 1105 62-63 0.7132967372834891 1105 64-65 0.8704746719389682 1105 66-67 0.6611601229596147 1105 68-69 0.8057642497687176 1105 70-71 1.1919347641126734 1105 72-73 1.1898653130445531 1105 74-75 1.058619243977649 1105 76-77 0.8162900844623877 1105 78-79 1.1629868453482928 1105 80-81 1.429224109894058 1105 82-83 1.3072896800913725 1105 84-85 1.0600636726394264 1105 86-87 1.0269961184854353 1105 88-89 1.0557376423812315 1105 90-91 1.2828543362623996 1105 92-93 1.6028804849674003 1105 94-95 1.4710532275695236 1105 96-97 1.4459456822573387 1105 98-99 2.2814049255142415 1105 100-101 2.4934810963007443 1106 1 0.12015484743109184 1106 2 0.23026985645431708 1106 3 0.08771987293274464 1106 4 0.06967331086689654 1106 5 -0.001447672541502243 1106 6 -0.023211025522911655 1106 7 -0.03254805566402297 1106 8 0.013242476306444928 1106 9 -0.08417676082276415 1106 10-11 0.1202350078542409 1106 12-13 0.06060765916312505 1106 14-15 0.14618014203167462 1106 16-17 0.16314812889496721 1106 18-19 0.17120845453058564 1106 20-21 -0.042264633125050466 1106 22-23 0.2736202720403824 1106 24-25 0.3920579658894283 1106 26-27 0.09084256852449357 1106 28-29 0.2839286328568207 1106 30-31 0.056701641163328986 1106 32-33 -0.019081228761088198 1106 34-35 0.22127168522930418 1106 36-37 0.5575885748819971 1106 38-39 0.44809778541763023 1106 40-41 0.4012377662002322 1106 42-43 0.7182037863373196 1106 44-45 1.1384536601154878 1106 46-47 0.6787633359711478 1106 48-49 0.0581127746427228 1106 50-51 0.049301784782183944 1106 52-53 0.5325914010168837 1106 54-55 0.7095994706113267 1106 56-57 0.4047927545862784 1106 58-59 0.5574059230645716 1106 60-61 0.5942158765333225 1106 62-63 0.6904408438843035 1106 64-65 0.45954448723968255 1106 66-67 0.21721301177990782 1106 68-69 0.24281300557808194 1106 70-71 0.2533296981731432 1106 72-73 0.661521604310348 1106 74-75 0.5917347796398715 1106 76-77 0.237004014938627 1106 78-79 0.40396624889136135 1106 80-81 0.7067489336837376 1106 82-83 0.38995418593075115 1106 84-85 0.047754674337216585 1106 86-87 0.056771804033616036 1106 88-89 -0.4368538069164991 1106 90-91 -0.23237006393653203 1106 92-93 0.10243031261569868 1106 94-95 0.16978931510017858 1106 96-97 0.770760039754208 1106 98-99 0.8775703145489011 1106 100-101 0.9004454769493329 1107 1 0.3154347137635902 1107 2 0.1921015189438755 1107 3 -0.13037745371721599 1107 4 -0.04530162647645852 1107 5 -0.00854875858995996 1107 6 -0.08518888684287873 1107 7 0.09177316405192926 1107 8 0.11505951891295751 1107 9 0.20158973875952313 1107 10-11 0.2919925266512351 1107 12-13 0.20920832750064022 1107 14-15 0.4039339014301717 1107 16-17 0.4616809192040776 1107 18-19 0.49441229747127124 1107 20-21 0.5330016576017655 1107 22-23 0.401387607044569 1107 24-25 0.36425408117765556 1107 26-27 0.5267239386163922 1107 28-29 0.18824671974068963 1107 30-31 0.10734909312657237 1107 32-33 0.13737762671092213 1107 34-35 -0.044000871161671284 1107 36-37 -0.12140056463346127 1107 38-39 0.07095701682949596 1107 40-41 0.14121270354358728 1107 42-43 -5.462136626803726E-4 1107 44-45 -0.8490933323657188 1107 46-47 -0.3992441828258464 1107 48-49 -0.4991658385569444 1107 50-51 -0.7162057340148102 1107 52-53 -0.6559789832771834 1107 54-55 -0.6539917992299458 1107 56-57 -0.8416305954554915 1107 58-59 -0.5396596575536421 1107 60-61 -0.18390129138647637 1107 62-63 -0.16145451952422007 1107 64-65 0.03324436192639979 1107 66-67 -0.08393047192936365 1107 68-69 -0.18635888247538546 1107 70-71 -0.4895692157784062 1107 72-73 -0.6182664073855548 1107 74-75 -0.6798556547795087 1107 76-77 -0.6938909307589505 1107 78-79 -0.36699761911197726 1107 80-81 -0.31204805879746544 1107 82-83 -0.11638979903165492 1107 84-85 -0.032054222905888 1107 86-87 -0.1825765668937045 1107 88-89 0.08648579208099605 1107 90-91 -0.11287862701088258 1107 92-93 -0.1478046497903165 1107 94-95 0.002783258291490398 1107 96-97 0.07412261285362476 1107 98-99 0.5330632134628566 1107 100-101 0.7169659447855921 1108 1 0.020697510524943397 1108 2 0.08623662255304154 1108 3 0.048528474648364295 1108 4 0.16917278000082803 1108 5 0.1776732806580199 1108 6 0.24678071656043699 1108 7 0.030919932269306116 1108 8 0.13905773491405427 1108 9 0.39512788888988837 1108 10-11 0.3284229850017226 1108 12-13 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-0.385331661848614 2316 54-55 -0.21574775161089477 2316 56-57 -0.47388505974120676 2316 58-59 -0.42098406231554364 2316 60-61 -0.2804414699579034 2316 62-63 -0.3781583885718405 2316 64-65 -0.40202349521645786 2316 66-67 0.06041476616587005 2316 68-69 -0.6389258467610972 2316 70-71 -0.3943311205403113 2316 72-73 -0.16253724071888698 2316 74-75 -0.31378422620808166 2316 76-77 -0.3333998593303775 2316 78-79 -0.2762238095881706 2316 80-81 -0.017963237368896046 2316 82-83 -0.07639723950784472 2316 84-85 -0.26029083004874565 2316 86-87 -0.9225319240365621 2316 88-89 -0.7152255174428142 2316 90-91 -0.3223280317727877 2316 92-93 -0.8805055426474588 2316 94-95 -0.7317777536132724 2316 96-97 -0.6433253038130431 2316 98-99 -0.5631049412990521 2316 100-101 -0.4028257218810758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 18.0 6 64.0 7 133.0 8 288.0 9 482.0 10 952.0 11 1794.0 12 2786.0 13 3818.0 14 4777.0 15 6748.0 16 9509.0 17 13938.0 18 11393.0 19 5001.0 20 3185.0 21 2876.0 22 2943.0 23 3174.0 24 3355.0 25 3804.0 26 4348.0 27 5215.0 28 6321.0 29 7391.0 30 9130.0 31 11096.0 32 13422.0 33 17201.0 34 23677.0 35 33080.0 36 43274.0 37 49312.0 38 28846.0 39 1723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.49502520978301 19.59258363707465 15.620512912364287 16.291878240778054 2 17.286179890642533 24.305174183997515 36.605202836608484 21.80344308875146 3 22.431541090268762 28.853770276521768 32.116518183581796 16.598170449627673 4 12.495411584592473 18.985146963588125 36.90190191683851 31.61753953498089 5 11.47312501865282 40.83743695347241 30.573014593965443 17.116423433909333 6 25.349241829636238 38.32811572058266 16.748387712895013 19.574254736886086 7 23.68278904032732 39.51468740580692 19.07557232088766 17.7269512329781 8 28.259565540478032 28.3825201517236 18.677760435474198 24.68015387232417 9 29.00991399120215 16.706656878017917 21.389689687897288 32.893739442882634 10-11 25.92327871005094 27.42812974695822 23.47537774223103 23.17321380075981 12-13 31.395504994285 26.839469623943916 24.720890048137328 17.044135333633754 14-15 25.68662092675546 26.5541671764906 30.417090690963132 17.342121205790804 16-17 19.156746238991534 29.019675722134515 29.81365810858802 22.00991993028593 18-19 18.72655319682332 25.953913694872966 37.309403790883614 18.0101293174201 20-21 22.22528952430124 22.076964743766702 37.40515197321201 18.29259375872005 22-23 20.740383725823154 23.47090123939442 37.503394681317765 18.285320353464666 24-25 20.793141526890707 23.71858771563302 37.980809407270335 17.507461350205933 26-27 20.21828730716313 22.77646293383632 40.05545225823203 16.94979750076851 28-29 18.55789333878523 25.542491228960323 38.26546068290994 17.634154749344503 30-31 19.1896168256385 23.992954487782306 37.941874524204025 18.875554162375174 32-33 18.45073511966492 24.59759789782486 38.00940965108489 18.942257331425342 34-35 20.218673009686135 24.01433745241616 37.94276658514056 17.82422295275714 36-37 19.77019076643872 23.48253969201563 38.16239175922416 18.58487778232149 38-39 19.375658470051366 24.99619467732356 37.37856543811948 18.24958141450559 40-41 19.390148903593204 25.747577220048413 35.95832251402034 18.903951362338045 42-43 18.690401931611635 26.411774712959286 34.515172045353474 20.3826513100756 44-45 20.32143017026547 24.634772357602255 34.203661976034496 20.840135496097773 46-47 21.59064456633978 26.899110057956655 32.313968687887595 19.19627668781597 48-49 21.400518091418068 26.830181809499937 30.633945731714597 21.135354367367405 50-51 20.054466637319347 27.77141919165541 31.127459392510463 21.046654778514775 52-53 21.695051325172837 29.33719720834036 27.080948877770133 21.88680258871667 54-55 20.76239761605255 27.291244185994458 27.941696548081097 24.004661649871895 56-57 22.692328353398313 27.441670297662036 26.28746142689165 23.578539922048 58-59 23.747491252023785 26.987338749990673 26.332564854399354 22.932605143586184 60-61 22.700793534736167 27.814750375280155 28.177048671523174 21.3074074184605 62-63 23.296501607813 27.53232412913238 27.753463699238246 21.41771056381637 64-65 22.76543607835412 29.549042958665005 27.11174386571276 20.573777097268113 66-67 23.7271266285363 27.80915516811732 26.174911252139427 22.288806951206954 68-69 23.49896217729384 27.843790895511923 26.826709721511357 21.83053720568288 70-71 24.190036754446574 28.3022668972711 26.75284984995292 20.75484649832941 72-73 23.251468551506758 27.22416901475715 28.373191174363626 21.15117125937246 74-75 21.11292009831207 30.449880691407845 27.38053844810785 21.05666076217224 76-77 20.6793379722827 28.88887562432568 27.701183215618624 22.730603187772992 78-79 20.48932493217086 28.714484841791705 28.539427459384598 22.256762766652837 80-81 20.987101664667158 28.64373496636644 27.932118108403316 22.437045260563085 82-83 20.156719602933233 28.084475178850166 28.240568022754335 23.518237195462266 84-85 19.713381451267534 28.313877751399296 27.415583466013288 24.557157331319882 86-87 19.44145181939997 28.720496115887705 26.617435083248033 25.220616981464296 88-89 19.171357116850405 28.40293901718406 25.97723515121852 26.448468714747015 90-91 19.55195306146507 27.082770650881216 26.990702123246486 26.374574164407232 92-93 20.258772479572166 28.14848305787837 27.61726980261548 23.975474659933987 94-95 19.650051629769784 27.915142146007792 27.671318319913574 24.76348790430885 96-97 17.864161405508806 28.728682425021972 28.617515440273483 24.78964072919573 98-99 19.049992315302397 28.94086960933385 26.405814704780777 25.603323370582977 100-101 18.971588874298675 27.915124746329234 27.622209621582904 25.491076757789184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2483.0 1 1599.5 2 670.0 3 583.5 4 490.5 5 484.5 6 698.5 7 1217.5 8 1732.5 9 1855.0 10 1462.0 11 1063.0 12 994.0 13 988.0 14 1037.0 15 1033.0 16 1055.0 17 1154.0 18 1224.0 19 1294.5 20 1461.0 21 1628.5 22 1796.5 23 1993.0 24 2181.5 25 2461.5 26 3018.0 27 3741.0 28 4090.0 29 4278.5 30 4512.0 31 4959.5 32 5677.5 33 6323.0 34 7096.0 35 7631.5 36 7821.0 37 8153.0 38 7743.0 39 6851.5 40 6594.5 41 6862.5 42 7345.0 43 7998.0 44 8538.5 45 8583.0 46 8934.5 47 13472.5 48 22858.5 49 21624.0 50 12167.0 51 7713.5 52 5917.5 53 5672.5 54 5526.5 55 5477.0 56 5988.5 57 6446.0 58 7100.0 59 7822.0 60 8348.0 61 8561.0 62 7366.0 63 4412.5 64 2130.0 65 1485.0 66 1064.5 67 778.5 68 590.0 69 448.5 70 349.5 71 318.5 72 286.5 73 296.5 74 248.5 75 170.5 76 140.5 77 76.5 78 26.0 79 17.0 80 9.0 81 5.0 82 7.5 83 6.5 84 3.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028052751109426618 2 0.010445173285424806 3 0.008356138628339844 4 0.0 5 0.003879635791729213 6 0.0 7 0.0 8 0.0 9 2.984335224407087E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0047749363590513395 20-21 0.0022382514183053154 22-23 0.0 24-25 0.006863971016136301 26-27 0.005968670448814174 28-29 0.009400655956882326 30-31 0.03521515564800363 32-33 4.4765028366106313E-4 34-35 0.004924153120271693 36-37 0.019696612481086773 38-39 0.007759271583458426 40-41 0.010146739762984096 42-43 0.007759271583458426 44-45 0.005371803403932757 46-47 0.0014921676122035435 48-49 0.002089034657084961 50-51 0.004625719597830985 52-53 0.0040288525529495675 54-55 0.002536684940746024 56-57 0.0014921676122035435 58-59 0.001641384373423898 60-61 0.0 62-63 1.4921676122035436E-4 64-65 0.011937340897628348 66-67 0.0013429508509831892 68-69 0.0019398178958646066 70-71 0.006416320732475238 72-73 0.017607577824001814 74-75 0.00820692186711949 76-77 0.006416320732475238 78-79 0.014921676122035436 80-81 2.984335224407087E-4 82-83 0.007759271583458426 84-85 0.0010445173285424806 86-87 0.0 88-89 0.0014921676122035435 90-91 0.0019398178958646066 92-93 2.984335224407087E-4 94-95 0.0014921676122035435 96-97 4.4765028366106313E-4 98-99 4.4765028366106313E-4 100-101 8.953005673221263E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 335083.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.792346381876456 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87740426371093 30.989246457830077 2 12.035038030015487 9.578048040188646 3 4.040983528013214 4.82400648810477 4 1.8016136730045456 2.8676174129008634 5 0.9376404847593431 1.8655457475607164 6 0.6314882419467025 1.5077039315775236 7 0.38169185004952005 1.0631890015816825 8 0.2726992479723943 0.8681074346715788 9 0.20958476380459642 0.7505882565908639 >10 1.4700674571690764 11.43673599821291 >50 0.16375798721497722 4.653485546573009 >100 0.14873431866314446 11.75955645476161 >500 0.015023668551832773 3.8730223681234888 >1k 0.01201893484146622 8.67865489203499 >5k 0.0022535502827749163 5.28449196928727 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6107 1.8225335215454082 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6042 1.8031353425867622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5530 1.6503373790971194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3201 0.9552857053327085 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2717 0.8108438804714057 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2558 0.7633929504033329 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2421 0.7225075578289558 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2361 0.7046015464825133 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1917 0.5720970625188386 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1842 0.5497145483357855 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1695 0.5058448205370013 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1521 0.4539173876323179 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1499 0.44735185013862233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1371 0.40915235926621163 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1361 0.4061680240418046 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1221 0.3643873309001054 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1212 0.36170142919813897 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1106 0.3300674758194238 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1031 0.3076849616363707 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 960 0.2864961815430804 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 817 0.24382018783405904 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 791 0.2360609162506006 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 786 0.23456874863839705 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 728 0.21725960433683594 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 726 0.21666273729195454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 703 0.20979876627581825 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 702 0.2095003327533775 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 637 0.19010215379473147 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 633 0.1889084197049686 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 622 0.18562565095812084 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 583 0.17398674358293317 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 579 0.17279300949317034 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 565 0.16861494017900044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 544 0.16234783620774554 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 530 0.15816976689357562 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 522 0.15578229871404994 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 515 0.15369326405696498 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 511 0.15249952996720215 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 503 0.15011206178767647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 476 0.14205435668177735 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 457 0.13638411975540388 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 436 0.130117015784149 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 427 0.1274311140821826 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 427 0.1274311140821826 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 425 0.1268342470373012 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 422 0.12593894646997908 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 422 0.12593894646997908 No Hit ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA 422 0.12593894646997908 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 419 0.12504364590265696 No Hit GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 409 0.12205931067824988 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.11579220670699499 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 384 0.11459847261723216 No Hit GGTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCA 374 0.11161413739282505 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 356 0.10624233398889231 No Hit GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT 355 0.10594390046645158 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 350 0.10445173285424805 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 347 0.10355643228692592 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 345 0.1029595652420445 No Hit GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG 340 0.10146739762984097 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 339 0.10116896410740026 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.984335224407087E-4 0.0 16-17 0.0 0.0 0.0 4.476502836610631E-4 0.0 18-19 0.0 0.0 0.0 5.968670448814174E-4 0.0 20-21 0.0 0.0 0.0 5.968670448814174E-4 0.0 22-23 0.0 0.0 0.0 0.0025366849407460245 0.0 24-25 0.0 0.0 0.0 0.004476502836610631 0.0 26-27 0.0 0.0 0.0 0.008057705105899135 0.0 28-29 0.0 0.0 0.0 0.019248962197425713 0.0 30-31 0.0 0.0 0.0 0.039691658484614256 0.0 32-33 0.0 0.0 0.0 0.06237260619010812 0.0 34-35 0.0 0.0 0.0 0.0837106030446188 0.0 36-37 0.0 0.0 0.0 0.10116896410740026 0.0 38-39 0.0 0.0 0.0 0.11758280784163924 0.0 40-41 0.0 0.0 0.0 0.1305646660678101 0.0 42-43 0.0 0.0 0.0 0.14861989417547294 0.0 44-45 0.0 0.0 0.0 0.1632431367750677 0.0 46-47 0.0 0.0 0.0 0.19069902083961288 0.0 48-49 0.0 0.0 0.0 0.21860255518781913 0.0 50-51 0.0 0.0 0.0 0.24068663584843158 0.0 52-53 0.0 0.0 0.0 0.2806767278554866 0.0 54-55 0.0 0.0 0.0 0.37080365163258056 0.0 56-57 0.0 0.0 0.0 0.5471778633950395 0.0 58-59 0.0 0.0 0.0 0.7169865376638027 0.0 60-61 0.0 0.0 0.0 0.8559073423599526 0.0 62-63 0.0 0.0 0.0 1.0313862535550893 0.0 64-65 0.0 0.0 0.0 1.2296953292169404 0.0 66-67 0.0 0.0 0.0 1.544841128914329 0.0 68-69 0.0 0.0 0.0 1.9262391705935544 0.0 70-71 0.0 0.0 0.0 2.2586941145925037 0.0 72-73 0.0 0.0 0.0 2.60651838499715 0.0 74-75 0.0 0.0 0.0 2.9798587215704764 0.0 76-77 0.0 0.0 0.0 3.3633457979067876 0.0 78-79 0.0 0.0 0.0 3.689235204412041 0.0 80-81 0.0 0.0 0.0 4.022883882500754 0.0 82-83 0.0 0.0 0.0 4.396821086118962 0.0 84-85 0.0 0.0 0.0 4.98563042589448 0.0 86-87 0.0 0.0 0.0 5.697543593676791 0.0 88-89 0.0 0.0 0.0 6.471829367649209 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACGC 25 0.005011252 57.01989 3 TTACGCC 25 0.0050201453 56.994358 4 CGTCACT 170 0.0 44.73484 1 TAAGGTG 45 9.61998E-4 42.218044 5 GTCACTA 220 0.0 38.883003 2 TGGACGG 25 0.0016161206 37.99624 82-83 GTAAGGT 50 0.0016149668 37.99624 4 TACGCCG 55 0.00257828 34.542034 5 TACATAG 60 0.003948905 31.663534 5 ACTACCT 290 0.0 31.117609 5 TCACTAC 275 0.0 31.101757 3 ATATATA 95 3.4681703E-5 30.019434 1 CTACATA 65 0.0058410643 29.227877 4 TAATACC 70 0.008387283 27.14017 4 GGTATAG 90 8.9083036E-4 26.40204 2 ATGGGTG 180 6.670234E-9 26.386278 5 CACTACC 325 0.0 26.305088 4 TATCAAG 95 0.0012211876 25.012457 2 TACCTCC 380 0.0 24.993795 7 AGTACTT 6000 0.0 23.310537 12-13 >>END_MODULE