##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344519 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77624456125787 33.0 31.0 34.0 30.0 34.0 2 31.769731132390376 33.0 31.0 34.0 30.0 34.0 3 31.690681791134885 33.0 31.0 34.0 30.0 34.0 4 35.37612729631748 37.0 35.0 37.0 33.0 37.0 5 35.425546921940445 37.0 35.0 37.0 33.0 37.0 6 35.58392135121721 37.0 35.0 37.0 33.0 37.0 7 35.56139138915415 37.0 35.0 37.0 33.0 37.0 8 35.540573959636475 37.0 35.0 37.0 33.0 37.0 9 37.08754524423907 39.0 37.0 39.0 33.0 39.0 10-11 36.95144970233862 39.0 37.0 39.0 33.0 39.0 12-13 36.872332150041075 39.0 37.0 39.0 32.5 39.0 14-15 37.688168141669976 40.0 37.0 41.0 32.5 41.0 16-17 37.693533593212564 40.0 37.0 41.0 32.5 41.0 18-19 37.8506453925618 40.0 37.0 41.0 33.0 41.0 20-21 37.879143095155854 40.0 37.5 41.0 33.0 41.0 22-23 37.754046946612526 40.0 37.0 41.0 33.0 41.0 24-25 37.600451644176374 40.0 37.0 41.0 32.0 41.0 26-27 37.472486277970155 40.0 36.5 41.0 32.0 41.0 28-29 37.346179746254926 39.5 36.0 41.0 32.0 41.0 30-31 36.95125232570628 39.0 35.5 41.0 32.0 41.0 32-33 36.4135998304883 38.0 35.0 40.5 31.0 41.0 34-35 36.03684557310337 38.0 35.0 40.0 30.5 41.0 36-37 35.63656576270104 38.0 35.0 40.0 30.0 41.0 38-39 35.24148885837936 38.0 35.0 40.0 28.5 41.0 40-41 34.842561658428124 38.0 35.0 40.0 26.5 41.0 42-43 34.21017418487804 38.0 34.5 40.0 23.0 41.0 44-45 33.3645052957892 38.0 33.5 40.0 13.0 41.0 46-47 32.172010832493996 37.0 32.5 40.0 2.0 41.0 48-49 31.17360290724169 37.0 31.0 40.0 2.0 41.0 50-51 30.138821080985373 36.0 29.5 39.0 2.0 40.5 52-53 30.17247379680076 36.0 30.0 39.0 2.0 40.5 54-55 30.396494823217296 36.0 30.0 40.0 2.0 41.0 56-57 30.140996577837505 35.5 29.5 40.0 2.0 41.0 58-59 29.878118478226163 35.0 29.0 40.0 2.0 41.0 60-61 29.481879083591906 35.0 28.0 39.0 2.0 41.0 62-63 29.183991303817788 35.0 28.0 39.0 2.0 41.0 64-65 28.8295986578389 35.0 26.5 38.5 2.0 40.5 66-67 28.403578612500326 35.0 26.0 38.0 2.0 40.0 68-69 28.000256879881803 35.0 25.5 37.0 2.0 39.5 70-71 27.55896626891405 34.5 24.5 36.5 2.0 39.0 72-73 27.227260325265078 34.0 24.0 36.0 2.0 39.0 74-75 26.890585134636986 34.0 23.0 35.5 2.0 38.5 76-77 26.542512894789546 34.0 20.5 35.0 2.0 37.0 78-79 26.110243556959123 34.0 19.0 35.0 2.0 37.0 80-81 25.68674441757929 34.0 16.5 35.0 2.0 36.0 82-83 25.309759403690364 33.0 10.0 35.0 2.0 36.0 84-85 24.956672926602018 33.0 6.0 35.0 2.0 35.5 86-87 24.68051544327018 33.0 2.0 35.0 2.0 35.0 88-89 24.27795854510201 33.0 2.0 35.0 2.0 35.0 90-91 23.840624174573826 32.0 2.0 35.0 2.0 35.0 92-93 23.473007294227607 32.0 2.0 35.0 2.0 35.0 94-95 23.063038613255003 32.0 2.0 35.0 2.0 35.0 96-97 22.58245699076103 31.0 2.0 35.0 2.0 35.0 98-99 22.1349272463928 31.0 2.0 35.0 2.0 35.0 100-101 20.38590034221625 28.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27953884084335456 1101 2 0.23453627762856044 1101 3 0.30891354360775836 1101 4 0.04271982301315802 1101 5 0.03761082533804938 1101 6 0.09785739338877875 1101 7 0.23342497947110274 1101 8 0.1621444844612654 1101 9 0.18318794837676933 1101 10-11 0.11803870447960918 1101 12-13 0.24696618946543225 1101 14-15 0.18906089677827964 1101 16-17 0.27366986408451055 1101 18-19 0.22988209171825957 1101 20-21 -0.01038454128132571 1101 22-23 0.03316786212783285 1101 24-25 0.044411165616907056 1101 26-27 0.2561092254791717 1101 28-29 0.037602453379534495 1101 30-31 -0.02486465189512188 1101 32-33 -0.46015121862699715 1101 34-35 0.09681269242005897 1101 36-37 -0.15418545705563247 1101 38-39 0.093623526432971 1101 40-41 0.28826402539373674 1101 42-43 0.34131043013916695 1101 44-45 0.24580385156635742 1101 46-47 0.4387356659743844 1101 48-49 0.2596796327833424 1101 50-51 0.5077424631043783 1101 52-53 0.46929791493878525 1101 54-55 0.6642983358016465 1101 56-57 0.20786443195600413 1101 58-59 0.6599728496425712 1101 60-61 0.7425635340061554 1101 62-63 0.6300580698942575 1101 64-65 0.622194369988673 1101 66-67 0.8169275264612956 1101 68-69 0.5345815596127359 1101 70-71 0.69479315492406 1101 72-73 1.050952887443625 1101 74-75 0.9108385970507307 1101 76-77 1.1931064698066223 1101 78-79 0.8427666645105916 1101 80-81 0.8249945093032132 1101 82-83 0.8027569509980914 1101 84-85 0.7322319785251352 1101 86-87 0.5039011654246011 1101 88-89 1.0167915968890213 1101 90-91 0.6706717477460984 1101 92-93 0.9883899879225702 1101 94-95 0.8236801026053442 1101 96-97 0.8561364320693805 1101 98-99 0.4825925082977953 1101 100-101 0.3043592252888736 1102 1 -0.0335500310690513 1102 2 0.1599246147816018 1102 3 0.15115953291257256 1102 4 0.17793754646617543 1102 5 0.21089832215496074 1102 6 -0.21752330434485856 1102 7 -0.09723455707078443 1102 8 -0.2368878557475469 1102 9 -0.11322152450328815 1102 10-11 -0.15545251018806994 1102 12-13 -0.16034218843117998 1102 14-15 -0.17126505178296725 1102 16-17 0.09096041666918353 1102 18-19 -0.08982761034439335 1102 20-21 -0.1146626161476334 1102 22-23 -0.5098038720203775 1102 24-25 -0.34227077988856536 1102 26-27 -0.3313621266980604 1102 28-29 -0.3940247429535404 1102 30-31 -0.053830783802432336 1102 32-33 0.054809311039413444 1102 34-35 -0.371038084254252 1102 36-37 -0.16901866306531588 1102 38-39 0.02162199854910085 1102 40-41 -0.11153225675548839 1102 42-43 0.0979949221177705 1102 44-45 0.6543218042019632 1102 46-47 0.3552750089843144 1102 48-49 -0.2345908026635648 1102 50-51 -0.31018470776396967 1102 52-53 -0.2802564522626376 1102 54-55 -0.525222927249029 1102 56-57 -0.3225022601753942 1102 58-59 -0.46792376354816057 1102 60-61 -0.7920685006258807 1102 62-63 -0.45938679896237744 1102 64-65 -0.06013310645644765 1102 66-67 -0.5663803421406932 1102 68-69 -0.4199638949327209 1102 70-71 -0.6100196793005388 1102 72-73 -0.5353725518229915 1102 74-75 -0.39562944471142814 1102 76-77 -0.8707463440461893 1102 78-79 -0.5113204248706182 1102 80-81 -0.6369993791612636 1102 82-83 -0.5230642865878536 1102 84-85 -0.11045964357825255 1102 86-87 -0.37966135049085636 1102 88-89 -0.3747923094007639 1102 90-91 -0.4255849367458815 1102 92-93 -0.6701279647130285 1102 94-95 -0.8475065538754301 1102 96-97 -0.6406804765122338 1102 98-99 -0.4935979678926792 1102 100-101 -0.43787403165026717 1103 1 -0.11732660774895365 1103 2 0.044428062760843545 1103 3 -0.28626071912682605 1103 4 -0.08058204465079655 1103 5 0.1536733361332523 1103 6 0.07842227349631514 1103 7 -0.05307031569790155 1103 8 0.013302105686697985 1103 9 -0.022097382913919716 1103 10-11 0.03034410921523545 1103 12-13 0.08022067088266027 1103 14-15 0.14645082325825598 1103 16-17 -0.3171412592924554 1103 18-19 0.0918687094216395 1103 20-21 0.08179334864140486 1103 22-23 0.19329357461121077 1103 24-25 -0.10026708101686665 1103 26-27 -0.2464816231927287 1103 28-29 -0.13939756914957968 1103 30-31 0.09604161946059975 1103 32-33 0.409983430406875 1103 34-35 0.2809131422514639 1103 36-37 -0.04572652388451104 1103 38-39 0.10555269488319396 1103 40-41 -0.25831868275654557 1103 42-43 -0.3086659142860029 1103 44-45 -0.6674477761624829 1103 46-47 -1.3229625790072532 1103 48-49 -0.986687546796265 1103 50-51 -0.9017317823578814 1103 52-53 -1.0123821565536169 1103 54-55 -0.7064783931204879 1103 56-57 -0.5561655768303524 1103 58-59 -0.5136327856410325 1103 60-61 -0.6775970235144371 1103 62-63 -0.5378552080174437 1103 64-65 -0.6960594731035208 1103 66-67 -1.0333664785401702 1103 68-69 -0.7723100978545396 1103 70-71 -0.8233427857792996 1103 72-73 -0.9920326713911685 1103 74-75 -0.8848410689175346 1103 76-77 -0.9613794532489273 1103 78-79 -1.10619361665281 1103 80-81 -1.2829650283992677 1103 82-83 -1.07056181350503 1103 84-85 -1.181924568812299 1103 86-87 -1.0503865013847502 1103 88-89 -1.0920615958626065 1103 90-91 -0.929785593367292 1103 92-93 -1.2457326155424084 1103 94-95 -0.7490025897745589 1103 96-97 -0.3110221203596346 1103 98-99 -0.2613002230574786 1103 100-101 -0.4285730897282889 1104 1 0.22641250556347714 1104 2 0.09042100440062484 1104 3 0.026427667901543828 1104 4 0.27806141727798206 1104 5 -0.0543513832742093 1104 6 -0.22386790690082847 1104 7 -0.3642818998908126 1104 8 0.08914918464642341 1104 9 0.14323637452689297 1104 10-11 0.17800167271775535 1104 12-13 -0.31542995543320984 1104 14-15 -0.6552751427222674 1104 16-17 0.012038092731906147 1104 18-19 -0.06311985482307136 1104 20-21 -0.004319403361755292 1104 22-23 -0.3950949684281184 1104 24-25 -0.33281430084085883 1104 26-27 -0.13868258955191948 1104 28-29 -0.05357639103958434 1104 30-31 0.031050934678482633 1104 32-33 -0.21468523562363373 1104 34-35 -0.11522975167622462 1104 36-37 0.3811490157165238 1104 38-39 0.41603723768018597 1104 40-41 -0.04946089508293028 1104 42-43 0.214690290557904 1104 44-45 0.51759561912084 1104 46-47 0.2616241433992528 1104 48-49 -0.18838094738092792 1104 50-51 -0.5204705170029165 1104 52-53 -0.4685551924058444 1104 54-55 -0.22060647357973195 1104 56-57 -0.20845802923185985 1104 58-59 -0.9184585229064481 1104 60-61 -0.6735452824017614 1104 62-63 -0.6977323683149237 1104 64-65 -0.7183554092450528 1104 66-67 -1.0345062673952334 1104 68-69 -1.1374855723247883 1104 70-71 -1.0292015276947488 1104 72-73 -1.1149897608021142 1104 74-75 -0.6711060452129516 1104 76-77 -0.6493423741377313 1104 78-79 -0.5911340144050428 1104 80-81 -0.5675184280751289 1104 82-83 -0.7542268594752493 1104 84-85 -0.6503702148946076 1104 86-87 -0.991552948424328 1104 88-89 -0.864421905461068 1104 90-91 -0.5593049887111974 1104 92-93 -0.5430395330383568 1104 94-95 -0.48779024824686346 1104 96-97 -0.2981625756693056 1104 98-99 -0.5262500117610749 1104 100-101 -0.1839615267407524 1105 1 0.05219172222061985 1105 2 0.2710373949294116 1105 3 0.3538573638324749 1105 4 0.18158772972640946 1105 5 0.1934585576894179 1105 6 0.31331548673847465 1105 7 0.019391051293091266 1105 8 0.09394192253761702 1105 9 0.3806197300782159 1105 10-11 0.16459946361472788 1105 12-13 0.306340500987794 1105 14-15 0.5243652432359625 1105 16-17 0.39434622451631896 1105 18-19 0.35514174614182537 1105 20-21 0.5833861130478226 1105 22-23 0.4637811186664038 1105 24-25 0.4830461701805362 1105 26-27 0.6275920882872228 1105 28-29 0.6806012259222598 1105 30-31 0.7417965069504291 1105 32-33 0.7757054836044546 1105 34-35 0.5649184740585582 1105 36-37 0.6028448831703415 1105 38-39 0.5427194569400129 1105 40-41 0.6608720573705682 1105 42-43 0.4482172378060554 1105 44-45 0.4622966284523926 1105 46-47 0.4600863410758862 1105 48-49 0.6458093655753352 1105 50-51 0.6908067000351075 1105 52-53 0.5104964168114492 1105 54-55 0.37239168550107493 1105 56-57 0.7510496835847427 1105 58-59 0.6686201287789473 1105 60-61 0.5367559300657021 1105 62-63 0.49753202576874145 1105 64-65 0.34405333829509743 1105 66-67 0.17281932138151035 1105 68-69 0.1989900196259562 1105 70-71 0.03253274514216997 1105 72-73 0.2721666538490659 1105 74-75 0.26289065382849586 1105 76-77 0.29916743351795105 1105 78-79 0.45222280651795543 1105 80-81 0.341895651782675 1105 82-83 0.16682786026067475 1105 84-85 0.1232046597324512 1105 86-87 0.062055887987781944 1105 88-89 0.5613144622149271 1105 90-91 0.09210267966942709 1105 92-93 -0.16200893569182284 1105 94-95 0.08045865672361785 1105 96-97 -0.11307878210617517 1105 98-99 0.015242084982400428 1105 100-101 0.19961620448504647 1106 1 0.1120827675049263 1106 2 0.12195670034310169 1106 3 0.033155967850181156 1106 4 0.15119957855173993 1106 5 -0.0260000735669621 1106 6 0.29477616089577907 1106 7 0.01738219872243718 1106 8 0.021044408067069753 1106 9 0.048988814177633344 1106 10-11 -0.10759154762122591 1106 12-13 0.18698401477737292 1106 14-15 0.12828931541846345 1106 16-17 0.2856128230862893 1106 18-19 0.21471784420106843 1106 20-21 0.2905958508755404 1106 22-23 0.13333338288158814 1106 24-25 -0.1808625802450763 1106 26-27 -0.3205778129725658 1106 28-29 -0.36693026880024604 1106 30-31 -0.25623720091473245 1106 32-33 -0.29937963726718664 1106 34-35 -0.1973304193701111 1106 36-37 -0.42062294114018073 1106 38-39 -0.31180046134395667 1106 40-41 -0.45413475229916145 1106 42-43 -0.5690282507877527 1106 44-45 0.2516480074105161 1106 46-47 0.16971351165810944 1106 48-49 -0.10553554419654176 1106 50-51 -0.017671338780008483 1106 52-53 -0.12161117597030469 1106 54-55 0.01033857007717387 1106 56-57 -0.13578211757289793 1106 58-59 -0.3366403410882661 1106 60-61 -0.4247779480412106 1106 62-63 -0.49673082748287456 1106 64-65 -0.5838407713405829 1106 66-67 -0.7972181318020084 1106 68-69 -0.44028475054769345 1106 70-71 -0.06928884151087544 1106 72-73 -0.1405418922864463 1106 74-75 0.18182153568072934 1106 76-77 -0.3252026720319279 1106 78-79 -0.42665019314156183 1106 80-81 -0.043327364901013965 1106 82-83 -0.1322443222384777 1106 84-85 -0.35101222824848577 1106 86-87 -0.2900891579774907 1106 88-89 -0.22122724701217678 1106 90-91 -0.299649107868877 1106 92-93 -0.14686593262747039 1106 94-95 -0.11675789077382603 1106 96-97 -0.44112610931080454 1106 98-99 -0.19217194293724305 1106 100-101 0.17469281326951602 1107 1 0.16749402291618054 1107 2 0.29073375662015977 1107 3 0.32081830551251755 1107 4 0.11764979500195238 1107 5 0.15403239396550106 1107 6 0.08227616089578049 1107 7 0.08724150608174597 1107 8 0.013685100707760967 1107 9 -0.028121575432756174 1107 10-11 0.07365845237877267 1107 12-13 0.12459223988559387 1107 14-15 -0.012619775490627205 1107 16-17 0.455742693216159 1107 18-19 0.5094689264521506 1107 20-21 0.5075601365898237 1107 22-23 0.2943723439205499 1107 24-25 0.1671352552527594 1107 26-27 0.35196006581531236 1107 28-29 0.009503713883738385 1107 30-31 0.2238223228947831 1107 32-33 0.5510532631657128 1107 34-35 0.13121395292426286 1107 36-37 0.11682294630571022 1107 38-39 -0.06131344835694108 1107 40-41 0.21420940354499152 1107 42-43 0.2318916626321581 1107 44-45 0.3559228991854084 1107 46-47 -0.21567609873149962 1107 48-49 -0.1876242887852868 1107 50-51 -0.10211397947265155 1107 52-53 -0.35659494220406884 1107 54-55 -0.684791300052698 1107 56-57 -0.5574541954949765 1107 58-59 -0.6848275705254956 1107 60-61 -0.7875485108117708 1107 62-63 -0.5165630785651238 1107 64-65 -0.18697929947911263 1107 66-67 -0.1870990841829645 1107 68-69 -0.23292544318838537 1107 70-71 -0.26520334367537757 1107 72-73 -0.09400509574965099 1107 74-75 0.15322305083224563 1107 76-77 -0.05864423047349021 1107 78-79 -0.00457227106363689 1107 80-81 0.09539016756651719 1107 82-83 0.2455965868524359 1107 84-85 0.40607651634026 1107 86-87 0.25617707578874516 1107 88-89 0.3437348741999493 1107 90-91 0.20673617351640416 1107 92-93 0.3533234613119234 1107 94-95 0.4670624555465217 1107 96-97 0.24794315475845607 1107 98-99 0.6197598752445721 1107 100-101 0.7253151076418121 1108 1 -0.28631959422121867 1108 2 0.01664281651918742 1108 3 0.04530322842151335 1108 4 -0.1678939353841855 1108 5 -0.16624492899806853 1108 6 -0.10092584483487599 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-1.7293074394089558 2316 46-47 -1.4609715256841511 2316 48-49 -1.8894740796931515 2316 50-51 -1.986072059871951 2316 52-53 -1.7894367403964324 2316 54-55 -2.0869058285836566 2316 56-57 -2.1774165926351365 2316 58-59 -1.7923961999602724 2316 60-61 -2.600411049594989 2316 62-63 -2.1014350939435182 2316 64-65 -2.1896266757100555 2316 66-67 -2.053355997713247 2316 68-69 -2.6024789303056117 2316 70-71 -2.1948918975671923 2316 72-73 -2.5297598509095067 2316 74-75 -2.561336675324508 2316 76-77 -2.5504323054768605 2316 78-79 -3.299257283878962 2316 80-81 -2.7937952162187223 2316 82-83 -3.1308493527131915 2316 84-85 -3.4016345992674566 2316 86-87 -3.3153273758502664 2316 88-89 -2.561393784912422 2316 90-91 -3.18168619259718 2316 92-93 -3.3803580778759894 2316 94-95 -3.6914681663339586 2316 96-97 -3.634408794519839 2316 98-99 -3.888532145529741 2316 100-101 -4.261233017273874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 701.0 3 226.0 4 215.0 5 256.0 6 293.0 7 373.0 8 531.0 9 752.0 10 1107.0 11 1694.0 12 2878.0 13 4087.0 14 4691.0 15 6891.0 16 9529.0 17 13174.0 18 10456.0 19 4199.0 20 3378.0 21 2990.0 22 3029.0 23 3335.0 24 3589.0 25 3890.0 26 4424.0 27 5103.0 28 6056.0 29 7201.0 30 8801.0 31 10492.0 32 12892.0 33 17042.0 34 23035.0 35 33698.0 36 43125.0 37 48340.0 38 36577.0 39 5469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.34657317480365 18.44954488044946 15.128260964243717 17.075620980503174 2 17.220725376566364 22.546657066208322 37.792513965182856 22.44010359204246 3 21.646494587069935 28.793188040168033 32.17245894214886 17.387858430613175 4 11.997734961450785 18.025060618820145 38.15428397194837 31.8229204477807 5 11.42042021103519 39.57403662901501 31.19480081810998 17.810742341839816 6 25.39893771574017 36.920479771271836 17.75839425390221 19.922188259085786 7 23.376034989994977 39.08523002843228 19.7454745053394 17.793260476233346 8 27.24623956402763 27.743273627273833 19.542066337053583 25.468420471644947 9 29.15965971635284 16.09696167016651 22.20237524724006 32.54100336624058 10-11 25.638750397562486 26.539892037277202 24.116757749019346 23.704599816140963 12-13 30.921189255285007 26.112875563281385 25.253158950274397 17.712776231159207 14-15 25.76795451740087 25.519775200946825 30.881261662903075 17.831008618749227 16-17 19.240240920469528 28.82188264129684 29.75364894611815 22.18422749211549 18-19 18.512371011260104 25.52924228826678 37.445580039092334 18.51280666138078 20-21 22.41648454573837 21.87934276731843 36.86921418147231 18.83495850547089 22-23 20.975794591565492 23.07926824842156 37.20051741183747 18.744419748175485 24-25 20.897160125055517 23.225985305686425 37.60475721870492 18.27209735055314 26-27 19.861775095715377 22.742089512381316 39.698732406222156 17.697402985681155 28-29 18.79653523833299 25.01777954525003 37.93777594579924 18.24790927061774 30-31 19.063756786921505 24.03988917416173 37.63738935195332 19.258964686963452 32-33 18.26231716202369 24.372639688359037 37.91883285582831 19.44621029378895 34-35 19.968649990203122 23.891610243905976 37.52392978178361 18.615809984107287 36-37 19.425189261994333 23.356149273141053 38.06357693558125 19.155084529283357 38-39 19.480821060911946 24.644599287964926 36.95935188262597 18.915227768497164 40-41 19.024749198829593 25.569817936928153 36.109998374436856 19.295434489805395 42-43 18.429355997933246 25.833086600368066 34.44696982891246 21.290587572786222 44-45 19.740065600417985 24.598415140344258 34.159966328989 21.50155293024876 46-47 21.025621674653785 26.17869282146102 32.71415011915371 20.08153538473149 48-49 20.80973763420885 26.257477816898344 30.94314101689602 21.989643531996784 50-51 19.883545082740465 27.350360365851888 31.089718470527792 21.676376080879855 52-53 21.273378016319256 28.621252187947206 27.416901547455595 22.688468248277943 54-55 20.741303057265487 26.211374136547118 28.64101696932574 24.406305836861655 56-57 22.716835660935473 26.69794971682695 26.507813021605912 24.07740160063166 58-59 23.485597263284088 26.244374101053552 26.98747897318001 23.282549662482346 60-61 23.07537125407828 26.706056172861008 28.72037804637338 21.498194526687335 62-63 23.544105373925035 26.84814398200225 28.213505569868282 21.394245074204434 64-65 22.89511419183299 28.64693998397715 27.85944012911166 20.5985056950782 66-67 23.689745722485203 27.203353786437077 26.48263228106542 22.624268210012293 68-69 23.244495809788194 26.975032958353417 27.732349132048295 22.04812209981009 70-71 23.92828412196404 27.390359857230393 27.282033856957405 21.399322163848158 72-73 22.640660535226274 26.622129787799143 29.203053045135313 21.53415663183927 74-75 20.72556157432405 29.66023955085666 28.153902426279764 21.46029644853953 76-77 20.289272333760582 28.143376189031517 28.638367486765155 22.92898399044275 78-79 20.249013613440987 27.578613339372136 29.58216113738572 22.590211909801155 80-81 20.634056155607936 27.85487204288426 28.53325316497979 22.977818636528013 82-83 19.837119837410178 27.3490600275822 28.99150758510561 23.822312549902012 84-85 20.15637995073756 27.377812741758266 27.962459522499756 24.50334778500442 86-87 19.54366231533959 28.177262380377215 27.614425578370344 24.66464972591285 88-89 19.592734558759474 27.224351714725444 26.980718413334493 26.202195313180592 90-91 19.551108942976114 26.29913874708899 28.071298732221777 26.078453577713123 92-93 20.566982544220195 26.67120616636347 28.923630418906775 23.83818087050956 94-95 20.01956583584438 26.68544870790007 28.94800248491921 24.346982971336338 96-97 18.22116808240096 27.339590433777893 30.05571006380965 24.383531420011494 98-99 19.5799119638564 27.421139127303977 27.938699898955875 25.06024900988374 100-101 19.76699266006547 26.608351898594464 29.55754165928413 24.067113782055937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1726.0 1 1150.5 2 595.5 3 583.0 4 484.0 5 479.0 6 697.5 7 1269.5 8 1955.0 9 2131.5 10 1638.5 11 1120.5 12 1017.0 13 1067.0 14 1084.0 15 1102.5 16 1134.0 17 1152.0 18 1230.5 19 1322.0 20 1443.0 21 1639.0 22 1835.5 23 1988.0 24 2177.0 25 2482.5 26 3009.0 27 3678.5 28 4071.0 29 4334.5 30 4651.5 31 5089.5 32 5762.5 33 6405.5 34 7214.0 35 7825.5 36 8061.5 37 8417.0 38 7863.5 39 6832.5 40 6509.5 41 6735.0 42 7293.0 43 7895.5 44 8401.5 45 8420.0 46 8922.0 47 13438.0 48 23334.5 49 22899.5 50 13098.5 51 8413.0 52 6757.0 53 6398.5 54 6112.0 55 5941.5 56 6341.0 57 6706.5 58 7072.0 59 7727.0 60 8540.0 61 9306.5 62 8171.0 63 4725.5 64 2210.5 65 1481.0 66 1097.5 67 824.0 68 607.0 69 430.5 70 324.5 71 304.0 72 281.5 73 276.5 74 217.5 75 171.0 76 132.0 77 64.0 78 26.0 79 17.0 80 13.5 81 8.0 82 5.0 83 3.5 84 2.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030187014359150006 2 0.02641363756425625 3 0.01915714372792212 4 0.04470000203181827 5 0.09027078332399664 6 0.10304221247594472 7 0.05572987266304615 8 0.08040195170658222 9 0.06327662625283366 10-11 0.06777565243136083 12-13 0.06371201588301371 14-15 0.06138993785538678 16-17 0.05021493734743221 18-19 0.05979350921139327 20-21 0.0570360415535863 22-23 0.03265422226350361 24-25 0.009288312110507693 26-27 0.0023220780276269233 28-29 0.006385714575974038 30-31 0.004208766425073798 32-33 0.005805195069067308 34-35 0.006240584699247357 36-37 0.006675974329427405 38-39 0.003918506671620433 40-41 0.006675974329427405 42-43 0.004934415808707212 44-45 0.0026123377810802887 46-47 0.0017415585207201923 48-49 0.0 50-51 0.0023220780276269233 52-53 0.0052246755621605774 54-55 0.012916559028674762 56-57 0.008707792603600963 58-59 0.00536980543888726 60-61 0.003192857287987019 62-63 0.009143182233781012 64-65 0.003192857287987019 66-67 0.02104383212536899 68-69 0.04208766425073798 70-71 0.05456883364923269 72-73 0.003047727411260337 74-75 0.011900649891587982 76-77 0.01988279311155553 78-79 0.023220780276269233 80-81 0.07067824996589447 82-83 0.027284416824616346 84-85 0.010884740754501203 86-87 0.029896754605696636 88-89 0.04324870326455144 90-91 0.03976558622311106 92-93 0.039039936839477644 94-95 0.011900649891587982 96-97 0.01654480594684183 98-99 0.03337987164713702 100-101 0.06908182132190097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 344519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.98823033895828 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52106616509803 32.18439546435622 2 11.751051647654393 9.63309623318107 3 3.89674887389422 4.7916252124875784 4 1.736283379978386 2.8466873244903645 5 0.9856481202986246 2.0199986093980646 6 0.588361737713456 1.4469543856817335 7 0.36719493212782595 1.053546932014744 8 0.2324115225696045 0.7620909616408761 9 0.21749939010686156 0.8023423590254687 >10 1.3835326245779056 11.107069604384604 >50 0.15018305413253685 4.313952535746772 >100 0.14239053717282976 11.518488466079202 >500 0.014168212654012911 4.161801285243218 >1k 0.012042980755910974 9.863698434362108 >5k 0.0014168212654012912 3.4942521919079663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6523 1.8933643717763027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5511 1.599621501281497 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4669 1.3552227888737631 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3733 1.083539659641413 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3106 0.901546794226153 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2826 0.8202740632592107 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2171 0.6301539247472563 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2053 0.5959032738397592 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1773 0.5146305428728168 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1506 0.43713118870076834 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1462 0.4243597595488202 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1460 0.42377924004191353 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1452 0.4214571620142866 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1443 0.41884482423320635 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1372 0.39823638173801734 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1362 0.3953337842034837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1303 0.37820845874973513 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1220 0.3541168992131058 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1059 0.307385078907114 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 985 0.2859058571515649 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 894 0.25949221958730867 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 879 0.2551383232855082 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 878 0.25484806353205486 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 849 0.24643053068190723 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 844 0.24497923191464044 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 815 0.23656169906449281 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 802 0.23278832226959909 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 777 0.2255318284332649 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 752 0.21827533459693077 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.2107285810071433 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 643 0.18663702147051395 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 610 0.1770584496065529 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 585 0.16980195577021875 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 584 0.1695116960167654 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 574 0.16660909848223174 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 561 0.16283572168733798 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 541 0.1570305266182707 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 519 0.15064481204229666 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 515 0.1494837730284832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 500 0.1451298767266827 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 483 0.1401954609179755 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 442 0.1282948110263875 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 437 0.1268435122591207 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 436 0.12655325250566732 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 428 0.12423117447804041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 426 0.12365065497113366 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.12103831719005337 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 412 0.11958701842278655 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 407 0.11813571965551974 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 397 0.11523312212098608 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 0.1114597453260923 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 384 0.1114597453260923 No Hit GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA 381 0.11058896606573224 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 379 0.1100084465588255 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 370 0.10739610877774519 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 369 0.10710584902429185 No Hit GTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAG 367 0.10652532951738511 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 357 0.10362273198285146 No Hit GAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCC 355 0.10304221247594472 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 349 0.10130065395522453 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.902597534533654E-4 0.0 10-11 0.0 0.0 0.0 5.805195069067308E-4 0.0 12-13 0.0 0.0 0.0 5.805195069067308E-4 0.0 14-15 0.0 0.0 0.0 5.805195069067308E-4 0.0 16-17 0.0 0.0 0.0 5.805195069067308E-4 0.0 18-19 0.0 0.0 0.0 7.256493836334135E-4 0.0 20-21 0.0 0.0 0.0 0.001015909137086779 0.0 22-23 0.0 0.0 0.0 0.002176948150900241 0.0 24-25 0.0 0.0 0.0 0.004644156055253847 0.0 26-27 0.0 0.0 0.0 0.009868831617414425 0.0 28-29 0.0 0.0 0.0 0.01988279311155553 0.0 30-31 0.0 0.0 0.0 0.037878897825664185 0.0 32-33 0.0 0.0 0.0 0.05674578180013294 0.0 34-35 0.0 0.0 0.0 0.07300032799352141 0.0 36-37 0.0 0.0 0.0 0.09244773147489688 0.0 38-39 0.0 0.0 0.0 0.1091376672984654 0.0 40-41 0.0 0.0 0.0 0.12408604460131371 0.0 42-43 0.0 0.0 0.0 0.1400503310412488 0.0 44-45 0.0 0.0 0.0 0.1545633187139171 0.0 46-47 0.0 0.0 0.0 0.17763896911345964 0.0 48-49 0.0 0.0 0.0 0.20289156766390243 0.0 50-51 0.0 0.0 0.0 0.22538669855653826 0.0 52-53 0.0 0.0 0.0 0.2651522847796493 0.0 54-55 0.0 0.0 0.0 0.36296982169343345 0.0 56-57 0.0 0.0 0.0 0.5239188549833246 0.0 58-59 0.0 0.0 0.0 0.6771760048067015 0.0 60-61 0.0 0.0 0.0 0.8212899723962974 0.0 62-63 0.0 0.0 0.0 0.9958812140984967 0.0 64-65 0.0 0.0 0.0 1.1935481062002387 0.0 66-67 0.0 0.0 0.0 1.5078994191902333 0.0 68-69 0.0 0.0 0.0 1.8882848260908687 0.0 70-71 0.0 0.0 0.0 2.209166983533564 0.0 72-73 0.0 0.0 0.0 2.5373056348125935 0.0 74-75 0.0 0.0 0.0 2.8867783779704457 0.0 76-77 0.0 0.0 0.0 3.2603426806649267 0.0 78-79 0.0 0.0 0.0 3.5767258119290952 0.0 80-81 0.0 0.0 0.0 3.895140761467437 0.0 82-83 0.0 0.0 0.0 4.2453391540089225 0.0 84-85 0.0 0.0 0.0 4.815409309791333 0.0 86-87 0.0 0.0 0.0 5.522191809450277 0.0 88-89 0.0 0.0 0.0 6.260032102728732 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 70 7.8649464E-8 47.5263 9 GTATAGC 60 8.2516846E-5 39.60525 1 CGTCACT 85 3.6150777E-7 39.139305 1 TCGCGCG 25 0.0016109789 38.021038 80-81 TAATACT 65 1.3219396E-4 36.55869 4 CTAGGTA 55 0.002570139 34.564583 4 TGCGTAC 55 0.002570139 34.564583 6 CTTTGCG 115 1.0278018E-7 33.061775 2 GCTAGTA 75 3.0640356E-4 31.684202 2 GTGTGCG 60 0.003936459 31.6842 3 GTGCGTA 60 0.003936459 31.6842 5 GATAGTC 60 0.003936459 31.6842 7 GTCACTA 185 9.094947E-12 30.827871 1 GTATATG 95 3.4706754E-5 30.016611 1 GCGTACT 65 0.0058226883 29.246952 7 GATAGAG 65 0.0058226883 29.246952 7 CATTCCG 85 6.3773134E-4 27.956648 9 CATGGGT 275 0.0 27.651665 4 CGTACTT 70 0.008360941 27.157885 8 CCTATGC 70 0.008360941 27.157885 8 >>END_MODULE