##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937028_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344519 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905500132068187 31.0 31.0 34.0 30.0 34.0 2 31.966474998476137 31.0 31.0 34.0 30.0 34.0 3 32.01191516287926 33.0 31.0 34.0 30.0 34.0 4 35.78186399008473 37.0 35.0 37.0 35.0 37.0 5 35.64716024370209 37.0 35.0 37.0 33.0 37.0 6 35.82906603119131 37.0 35.0 37.0 35.0 37.0 7 35.78925400340765 37.0 35.0 37.0 35.0 37.0 8 35.7633454178144 37.0 35.0 37.0 33.0 37.0 9 37.34102037913729 39.0 37.0 39.0 34.0 39.0 10-11 37.2576926091159 39.0 37.0 39.0 34.0 39.0 12-13 37.14565814947797 39.0 37.0 39.0 33.0 39.0 14-15 37.963539021069955 40.0 37.0 41.0 33.0 41.0 16-17 38.03637825490031 39.5 37.0 41.0 33.0 41.0 18-19 38.32735785254224 40.0 37.5 41.0 33.5 41.0 20-21 38.3975455635248 40.0 38.0 41.0 34.0 41.0 22-23 38.27441302221358 40.0 37.5 41.0 34.0 41.0 24-25 38.10459945605322 40.0 37.0 41.0 33.0 41.0 26-27 37.774894853404305 40.0 36.5 41.0 33.0 41.0 28-29 37.542586911026675 39.5 36.0 41.0 33.0 41.0 30-31 37.182050917365956 39.0 35.5 41.0 33.0 41.0 32-33 36.63953657127763 38.0 35.0 41.0 31.5 41.0 34-35 36.250306949689275 38.0 35.0 40.0 31.0 41.0 36-37 35.848623733379 38.0 35.0 40.0 30.5 41.0 38-39 35.46335325482775 38.0 35.0 40.0 30.0 41.0 40-41 34.92020323987937 38.0 35.0 40.0 27.0 41.0 42-43 34.30853450753079 38.0 34.0 40.0 23.5 41.0 44-45 33.43966515634841 37.5 33.0 40.0 14.5 41.0 46-47 32.16311437105065 37.0 32.5 40.0 2.0 41.0 48-49 31.038312836157075 37.0 31.0 40.0 2.0 41.0 50-51 30.38838061180951 36.0 29.5 40.0 2.0 41.0 52-53 30.018557757337042 36.0 29.0 40.0 2.0 41.0 54-55 29.56265111648414 35.0 28.0 39.0 2.0 41.0 56-57 29.23516845224792 35.0 27.0 39.0 2.0 40.5 58-59 28.89092909244483 35.0 26.0 39.0 2.0 40.0 60-61 28.39519300822306 35.0 26.0 38.0 2.0 40.0 62-63 28.035482803560907 34.5 25.0 38.0 2.0 40.0 64-65 27.706489337307957 34.0 24.0 37.5 2.0 40.0 66-67 27.355484022651872 34.0 23.0 37.0 2.0 39.0 68-69 26.890458871644235 34.0 21.5 36.0 2.0 39.0 70-71 26.438907578391905 33.0 20.0 36.0 2.0 38.5 72-73 26.04927159314871 33.0 19.0 35.0 2.0 37.5 74-75 25.64109091225738 33.0 17.5 35.0 2.0 37.0 76-77 24.366239597816087 30.5 13.0 34.0 2.0 35.5 78-79 24.959084985153215 32.0 10.0 35.0 2.0 36.0 80-81 24.78611049027775 32.0 7.0 35.0 2.0 36.0 82-83 24.4503423613792 32.0 2.0 35.0 2.0 35.0 84-85 24.048426937266157 32.0 2.0 35.0 2.0 35.0 86-87 23.71630011697468 32.0 2.0 34.0 2.0 35.0 88-89 23.327353498645937 31.0 2.0 34.0 2.0 35.0 90-91 22.83073067087737 31.0 2.0 34.0 2.0 35.0 92-93 22.379664111413305 31.0 2.0 34.0 2.0 35.0 94-95 21.797928416139605 30.0 2.0 34.0 2.0 35.0 96-97 21.047202041106587 29.0 2.0 34.0 2.0 35.0 98-99 20.328108174004917 29.0 2.0 34.0 2.0 35.0 100-101 17.82077331003515 23.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2698938579738943 1101 2 0.3321257910630244 1101 3 0.05787235522077694 1101 4 0.20139629611374943 1101 5 -0.29521595754408025 1101 6 0.0013925107442460671 1101 7 0.021945545645486675 1101 8 0.1440162928217319 1101 9 0.2949745631916514 1101 10-11 0.2144433739425935 1101 12-13 0.22755096457645863 1101 14-15 0.3593909217062219 1101 16-17 0.1838984099375054 1101 18-19 0.051642577761050745 1101 20-21 -0.11386913832455292 1101 22-23 0.15542201454857008 1101 24-25 0.13768145475538063 1101 26-27 0.1827256102012882 1101 28-29 0.29661442205105004 1101 30-31 0.243639530743593 1101 32-33 0.23509673574741896 1101 34-35 0.05636524493112205 1101 36-37 0.05265449572333125 1101 38-39 -0.034341478089665145 1101 40-41 0.27849899076321094 1101 42-43 0.025477276634532586 1101 44-45 -0.1714207899439799 1101 46-47 0.08297161287818966 1101 48-49 0.03843807207027439 1101 50-51 0.43417986479409265 1101 52-53 0.3816799727444753 1101 54-55 -0.35766431623774864 1101 56-57 -0.1826620920282096 1101 58-59 0.05143967632236013 1101 60-61 0.3738162549576529 1101 62-63 0.3349542722768426 1101 64-65 0.4169172602656417 1101 66-67 0.6539628717161889 1101 68-69 0.4328515955865875 1101 70-71 0.10400910876468217 1101 72-73 0.0524352725829722 1101 74-75 0.1312653741172447 1101 76-77 0.24015058174645176 1101 78-79 0.3223439598171396 1101 80-81 0.09536976268237041 1101 82-83 0.5995524914475894 1101 84-85 0.8439489011270851 1101 86-87 0.1992884448243153 1101 88-89 0.6564941035176091 1101 90-91 0.5333761723832886 1101 92-93 0.23518233359884277 1101 94-95 0.5834132481276306 1101 96-97 0.20678490274591255 1101 98-99 0.2698349815007255 1101 100-101 0.21935463987801285 1102 1 -0.1540939189550592 1102 2 -0.09708225579208474 1102 3 0.21129736158695422 1102 4 0.09355315885884607 1102 5 -0.2560002712695706 1102 6 -0.07334647575945041 1102 7 0.046646335052159316 1102 8 0.07462489990093246 1102 9 0.23296794236150475 1102 10-11 0.03803644753566715 1102 12-13 -0.18650556712712785 1102 14-15 5.2919519744421E-4 1102 16-17 0.08846932921431261 1102 18-19 0.17922088180994677 1102 20-21 0.09169490547478887 1102 22-23 0.028671314268457593 1102 24-25 -0.2665711553795802 1102 26-27 -0.21382391870118056 1102 28-29 -0.20497712365813925 1102 30-31 -0.30168769105422655 1102 32-33 -0.6198179574025602 1102 34-35 -0.21585273316920706 1102 36-37 -0.5125352165252082 1102 38-39 -0.025365160289815947 1102 40-41 -0.07883485186474815 1102 42-43 0.031143179359261808 1102 44-45 0.2865624033333276 1102 46-47 0.3572267694862816 1102 48-49 0.6738467810083932 1102 50-51 0.5603576086188973 1102 52-53 0.3384535912827964 1102 54-55 0.7676858238182724 1102 56-57 0.4896068155348168 1102 58-59 0.4771463226172443 1102 60-61 0.6112748747692116 1102 62-63 0.539117755852093 1102 64-65 0.8369580038357967 1102 66-67 0.6160840838374 1102 68-69 0.8495819240816225 1102 70-71 0.6264817341784834 1102 72-73 0.20471436603853732 1102 74-75 0.20250296006070911 1102 76-77 0.24524352801586957 1102 78-79 0.04140431123043342 1102 80-81 0.590658787383159 1102 82-83 0.9978845515443524 1102 84-85 0.7562229016363808 1102 86-87 0.6661842940731049 1102 88-89 0.8214419008183462 1102 90-91 0.38402552303263704 1102 92-93 0.6193814677979752 1102 94-95 0.6066498154818447 1102 96-97 1.0051042304770057 1102 98-99 0.9833567538460244 1102 100-101 0.37131542419173513 1103 1 0.051609225085886834 1103 2 0.066019348485991 1103 3 0.16086797706200073 1103 4 0.08779696898343303 1103 5 -0.25773501342380456 1103 6 -0.07402746958032225 1103 7 -0.16291338452175808 1103 8 -0.011674938094223819 1103 9 0.054784117040000524 1103 10-11 -0.0430269063947506 1103 12-13 -0.30331392829757675 1103 14-15 -0.15778753144446966 1103 16-17 -0.2531064188036325 1103 18-19 -0.4214013314916585 1103 20-21 -0.4352545289419254 1103 22-23 -0.4035554638422525 1103 24-25 -0.5405313853404436 1103 26-27 -0.4550211775218429 1103 28-29 -0.42267651148053176 1103 30-31 -0.3517299843546766 1103 32-33 -0.38125865359329225 1103 34-35 -0.4330610886049797 1103 36-37 -0.06942328981448043 1103 38-39 -0.2160613510254379 1103 40-41 -0.07267364987480818 1103 42-43 -0.43195770808321754 1103 44-45 -0.7237671976759756 1103 46-47 -0.42315630745885 1103 48-49 0.005286473629290356 1103 50-51 -0.2589998199484391 1103 52-53 -0.8149241380687542 1103 54-55 -0.4394187338224782 1103 56-57 -0.9014198364128276 1103 58-59 -0.997932887716658 1103 60-61 -0.9304807989845685 1103 62-63 -0.5789056416619225 1103 64-65 -0.6819592876725693 1103 66-67 -0.7568030432288779 1103 68-69 -0.8643863344379668 1103 70-71 -0.6671663855617354 1103 72-73 -0.5360778806676016 1103 74-75 -0.9804227327608963 1103 76-77 -1.124700988908618 1103 78-79 -1.457445167630084 1103 80-81 -1.6122773712382248 1103 82-83 -1.2424094944191424 1103 84-85 -1.2270891655741245 1103 86-87 -1.5528161503649685 1103 88-89 -1.1608773490848812 1103 90-91 -1.3822860710663711 1103 92-93 -1.3619557173319876 1103 94-95 -1.2997995544196606 1103 96-97 -0.903742954509589 1103 98-99 -1.4529667397239585 1103 100-101 -0.23430671742630693 1104 1 0.0903847662015913 1104 2 0.20443742758203953 1104 3 0.09772897583512119 1104 4 0.06829559820747022 1104 5 0.023826913841766384 1104 6 0.03377156406048698 1104 7 0.028805916912638452 1104 8 0.12322153424033644 1104 9 -0.089775947181991 1104 10-11 -0.0021686946136441065 1104 12-13 0.17538603080634374 1104 14-15 0.1727247298057364 1104 16-17 0.051308085672211234 1104 18-19 -0.023156833528886978 1104 20-21 -0.13723950341974245 1104 22-23 -0.10243536569166167 1104 24-25 -0.20793645342483558 1104 26-27 -0.19216163283151388 1104 28-29 -0.20084557889848043 1104 30-31 -0.28421737473046704 1104 32-33 -0.520517374303374 1104 34-35 -0.459848020035885 1104 36-37 -0.5856567609827437 1104 38-39 -0.4126351684471672 1104 40-41 -0.3063417251841827 1104 42-43 -0.6601919062928729 1104 44-45 -0.8859201490096424 1104 46-47 -0.7576256429733057 1104 48-49 -0.5477913349467691 1104 50-51 -0.23457577305426724 1104 52-53 -0.3089979933572202 1104 54-55 -0.44982830047551303 1104 56-57 -0.44276274245787306 1104 58-59 -0.79853421153787 1104 60-61 -0.36611870207600816 1104 62-63 -0.1316577962793879 1104 64-65 -0.12242898670772462 1104 66-67 -0.8379779724180736 1104 68-69 -0.771048608562868 1104 70-71 -0.5292102806860157 1104 72-73 -0.4981391944919942 1104 74-75 -0.7159311316036892 1104 76-77 -0.20471719587306936 1104 78-79 -0.45694911334432575 1104 80-81 -0.9839280581408758 1104 82-83 -0.7170690724321993 1104 84-85 -1.012161339579368 1104 86-87 -0.9107742243111367 1104 88-89 -0.5107660208711273 1104 90-91 -1.4046312339690807 1104 92-93 -1.2627524335283482 1104 94-95 -1.2834979231205317 1104 96-97 -1.0584550915568904 1104 98-99 -1.2679801445496963 1104 100-101 -1.110156825725177 1105 1 0.1663861627609755 1105 2 0.17837889904778947 1105 3 0.02707576692253255 1105 4 0.14510636441072933 1105 5 -0.09208751515588887 1105 6 -0.024391960347564634 1105 7 -0.02072433129405482 1105 8 0.13244200018333174 1105 9 0.03823141649477435 1105 10-11 -0.004826918444031492 1105 12-13 0.06840640787738295 1105 14-15 0.01615137869801231 1105 16-17 0.010268283729722327 1105 18-19 -0.04992951174890692 1105 20-21 0.14102495414389438 1105 22-23 0.0638662911356036 1105 24-25 -0.028843582225704267 1105 26-27 0.12915811578779568 1105 28-29 0.35925408465767106 1105 30-31 0.4609978223748499 1105 32-33 0.7908201487081143 1105 34-35 0.666627889314718 1105 36-37 0.6758172439686945 1105 38-39 0.6787521863424004 1105 40-41 0.5626435474622937 1105 42-43 0.6090663302335031 1105 44-45 0.4990541416016754 1105 46-47 0.4162380655266631 1105 48-49 0.37127963566200606 1105 50-51 0.7382627338967893 1105 52-53 0.8671668641302404 1105 54-55 0.6539217788744516 1105 56-57 0.6978850706570903 1105 58-59 0.650792900758205 1105 60-61 0.5428827579633477 1105 62-63 1.071676489253523 1105 64-65 0.9099616690403849 1105 66-67 0.896802704278425 1105 68-69 0.7299670709902379 1105 70-71 0.6768933186284016 1105 72-73 0.5469077806873379 1105 74-75 0.31440436004869454 1105 76-77 0.008424745344473905 1105 78-79 0.296248690248639 1105 80-81 0.5358459531585602 1105 82-83 0.4814962801990461 1105 84-85 0.21280374643599487 1105 86-87 0.28312535037305153 1105 88-89 1.1268030135984937 1105 90-91 0.6985872006148064 1105 92-93 0.36601668981323954 1105 94-95 0.27406254155286547 1105 96-97 0.330946927106222 1105 98-99 0.32892996780985584 1105 100-101 0.21781244996833848 1106 1 -0.2177302825914218 1106 2 -0.30064731818067614 1106 3 -0.348239180302528 1106 4 -0.10787286609659219 1106 5 -0.2846990234977298 1106 6 -0.14054790178440157 1106 7 -0.014850991151050152 1106 8 0.07052507815404851 1106 9 0.15614084788735028 1106 10-11 0.02921291812389626 1106 12-13 0.2724070888443535 1106 14-15 0.04660762656998685 1106 16-17 0.15379909748525478 1106 18-19 -0.2780874960866626 1106 20-21 -0.20465090557690502 1106 22-23 0.06289591319893617 1106 24-25 0.022109771536641176 1106 26-27 -0.024519105866957602 1106 28-29 -0.19401455681321522 1106 30-31 -0.20560925968166543 1106 32-33 -0.22819585401575182 1106 34-35 -0.013802822295765793 1106 36-37 -0.17661721296014576 1106 38-39 -0.10174662196539686 1106 40-41 0.08667316952563198 1106 42-43 0.18595601358386205 1106 44-45 0.12435206465240611 1106 46-47 0.012841742748314289 1106 48-49 0.12411416068754022 1106 50-51 0.28522391877932307 1106 52-53 0.1544072454717451 1106 54-55 0.11768582381827386 1106 56-57 0.4107298101872239 1106 58-59 0.18926753473845537 1106 60-61 0.03159573038418273 1106 62-63 0.2997238164581546 1106 64-65 0.38704713039550853 1106 66-67 0.3560127825896302 1106 68-69 0.36277265491941435 1106 70-71 0.7011697912194848 1106 72-73 0.3474951147016405 1106 74-75 0.2580288067630292 1106 76-77 0.3906089469106995 1106 78-79 0.5122599262036935 1106 80-81 0.2616035289161367 1106 82-83 0.32907884744453497 1106 84-85 0.19829063782175993 1106 86-87 -0.036043869919765825 1106 88-89 0.10379484199481936 1106 90-91 0.024310728023724693 1106 92-93 0.2702727333951245 1106 94-95 0.19105266753175343 1106 96-97 0.020255745628521282 1106 98-99 -0.1604043869739371 1106 100-101 0.6304954598423578 1107 1 0.13053811567697338 1107 2 0.2981622056288451 1107 3 0.07502921796587358 1107 4 0.148350077626354 1107 5 0.13212998083127303 1107 6 0.24018802895152902 1107 7 0.17670745040739178 1107 8 0.1335120911410641 1107 9 -0.03660806986156473 1107 10-11 0.03806032072130705 1107 12-13 -0.018772564835295213 1107 14-15 0.1360015659639302 1107 16-17 0.1569376256237902 1107 18-19 0.12053263378346202 1107 20-21 0.08282203814603406 1107 22-23 0.04390240670543477 1107 24-25 0.3164279533548182 1107 26-27 0.2155512404533937 1107 28-29 0.2468945340958726 1107 30-31 0.3849210433486263 1107 32-33 -0.10392637349627165 1107 34-35 0.07964955865660528 1107 36-37 0.0802280251350922 1107 38-39 0.05967112695234533 1107 40-41 0.02333442493688409 1107 42-43 0.049024195402040505 1107 44-45 -0.3828178487675089 1107 46-47 -0.9423260061694307 1107 48-49 -1.16149189991852 1107 50-51 -1.3133691548137527 1107 52-53 -1.7269509796364808 1107 54-55 -1.5641594142769648 1107 56-57 -1.3241186746612605 1107 58-59 -1.5385463180753973 1107 60-61 -1.2349897674513102 1107 62-63 -1.4143454476111081 1107 64-65 -1.3807017873534093 1107 66-67 -1.6764818061549605 1107 68-69 -1.6840076481108888 1107 70-71 -1.643364840815149 1107 72-73 -1.316087136380613 1107 74-75 -1.4471659984317746 1107 76-77 -1.4574159448641915 1107 78-79 -1.1883353118915458 1107 80-81 -1.1858802373176296 1107 82-83 -0.8769005897849027 1107 84-85 -0.6528998383687146 1107 86-87 -0.4606380257639202 1107 88-89 -0.6350850281350517 1107 90-91 -0.7132974970845005 1107 92-93 -0.8775627644403734 1107 94-95 -0.23862806839898099 1107 96-97 -0.2642410076182351 1107 98-99 -0.28583728740683867 1107 100-101 -0.06495908561218755 1108 1 0.1272293423108941 1108 2 0.11186462069229819 1108 3 -0.007195505734497232 1108 4 -0.06311294597850292 1108 5 -0.2163913044736816 1108 6 -0.1001380728358896 1108 7 -0.29187625814766704 1108 8 -0.01633782017016472 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-1.028322010651923 2316 72-73 -1.4160382358005492 2316 74-75 -1.117205118895825 2316 76-77 -1.499162707308546 2316 78-79 -1.8565786007084846 2316 80-81 -1.6178367663881836 2316 82-83 -1.5367422530791153 2316 84-85 -1.2799907597230913 2316 86-87 -1.4880097038969282 2316 88-89 -1.980276752133431 2316 90-91 -1.3950643256289474 2316 92-93 -1.0514802067714122 2316 94-95 -1.2877352112561233 2316 96-97 -1.0161422284465083 2316 98-99 -1.575345583643184 2316 100-101 -1.8831838259332425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 26.0 6 76.0 7 125.0 8 289.0 9 551.0 10 1126.0 11 1955.0 12 3105.0 13 4065.0 14 5064.0 15 7263.0 16 10046.0 17 14465.0 18 11888.0 19 5174.0 20 3467.0 21 3219.0 22 3336.0 23 3493.0 24 3638.0 25 4303.0 26 4914.0 27 5767.0 28 7074.0 29 8500.0 30 10011.0 31 12041.0 32 14787.0 33 18906.0 34 25599.0 35 34623.0 36 44990.0 37 47863.0 38 21980.0 39 776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.454111048533655 19.572550818377643 15.787808234289994 16.185529898798706 2 17.530629838295354 24.065575864180868 36.52824098665668 21.875553310867097 3 22.159719259136704 28.817445873498258 32.31335463852292 16.709480228842118 4 12.484071996029247 19.130439830604406 36.94542245855816 31.44006571480818 5 11.397604140361265 40.783313159812025 30.677975449259964 17.141107250566744 6 25.48016219715023 38.176994592460794 16.659168289702457 19.68367492068652 7 23.56038418780967 39.46081348198503 19.27556970733109 17.70323262287421 8 28.17087011166293 28.42658895445534 18.696501499191626 24.706039434690105 9 28.851238973757614 16.66381244575771 21.471094482452347 33.01385409803233 10-11 25.9754904664184 27.34769345086918 23.47156470325294 23.20525137945948 12-13 31.401658913941116 26.698354874057518 24.766728833607885 17.13325737839348 14-15 25.560212706669144 26.476436234441763 30.465557077837634 17.49779398105146 16-17 19.30633724119715 28.925690600518404 29.83536466784125 21.9326074904432 18-19 18.644626180092448 25.806961910498018 37.392875543332345 18.155536366077197 20-21 22.08558951458058 21.97282278207285 37.55843334063826 18.383154362708318 22-23 20.709983600133523 23.333091447397063 37.60605488875666 18.350870063712758 24-25 20.714017221286124 23.582973770410913 38.157499444872585 17.545509563430382 26-27 20.159324041124123 22.714488067386903 40.183386644529456 16.942801246959522 28-29 18.528586231818856 25.344320632534057 38.53619103735934 17.59090209828776 30-31 19.081531062147516 23.952234854971714 38.12721504474354 18.839019038137227 32-33 18.391755449997603 24.43424663697305 38.252372514103016 18.921625398926327 34-35 20.315280144201044 23.816103030602086 38.086288419506616 17.78232840569025 36-37 19.72471605174781 23.312927954208355 38.36690206869327 18.595453925350565 38-39 19.249297150429403 24.782831456890683 37.65749708627669 18.310374306403222 40-41 19.32940802545016 25.488329296274635 36.12410436074905 19.058158317526157 42-43 18.68242390114914 26.136439499708292 34.784583723974585 20.396552875167988 44-45 20.29757645860213 24.44367686308203 34.45321196517438 20.805534713141462 46-47 21.502328612251592 26.665766860125075 32.665154411156436 19.166750116466897 48-49 21.277055837268772 26.69722134338019 30.92093614575684 21.1047866735942 50-51 20.081650779644487 27.698133033008627 31.195067863321295 21.02514832402559 52-53 21.6789833912887 29.23098357705548 27.310619474163904 21.779413557491917 54-55 20.784339905781685 27.123612921203183 28.11876848591805 23.973278687097082 56-57 22.704183816230213 27.36141507845744 26.381785567082126 23.552615538230224 58-59 23.66284588077871 26.904031417715714 26.34600704170162 23.08711565980396 60-61 22.724409677372538 27.6506102782172 28.246520470806786 21.37845957360347 62-63 23.25314423877928 27.490356119691512 27.733158403455256 21.523341238073954 64-65 22.86085818198706 29.4037484144561 27.211271366746104 20.52412203681074 66-67 23.875316020306574 27.595139890688174 26.180704112109925 22.348839976895324 68-69 23.601455942923323 27.661319114475546 26.934363561951592 21.802861380649542 70-71 24.296408315385555 28.10317603144103 26.83502748767844 20.76538816549498 72-73 23.33111284299682 27.01901349274422 28.47931243169815 21.170561232560807 74-75 21.21790015413151 30.386112489876954 27.403624267439934 20.9923630885516 76-77 20.70408308395183 29.030619501391797 27.731561974811257 22.533735439845117 78-79 20.543629070179207 28.590233311467035 28.579638799714385 22.286498818639373 80-81 20.858803996354286 28.631637611243665 28.079410415832157 22.43014797656989 82-83 20.219494425561436 27.99192497365893 28.247063296944436 23.541517303835203 84-85 19.763523319671656 28.28823625027575 27.510623599484497 24.4376168305681 86-87 19.55816596206009 28.68539715574055 26.6286135577625 25.12782332443686 88-89 19.164879119220465 28.281080830041244 26.17869282146102 26.375347229277267 90-91 19.610700228726678 26.893961383506138 27.048671179277594 26.446667208489593 92-93 20.177551891187424 28.093806147121057 27.71530742861787 24.013334533073646 94-95 19.647102191751397 27.714581779234237 27.83213697938285 24.806179049631517 96-97 17.8605793302827 28.521835547292817 28.757091418893328 24.860493703531162 98-99 19.081814659094704 28.712122641440974 26.587986351887356 25.618076347576967 100-101 18.977478222756314 27.688956230715423 27.7081138192975 25.625451727230768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2565.0 1 1697.0 2 759.0 3 650.5 4 541.0 5 503.0 6 697.5 7 1225.5 8 1718.0 9 1790.5 10 1471.5 11 1119.0 12 1010.5 13 985.0 14 1027.0 15 1080.0 16 1106.5 17 1153.5 18 1228.5 19 1375.0 20 1484.5 21 1618.5 22 1832.5 23 1998.0 24 2206.0 25 2535.5 26 3111.0 27 3772.0 28 4173.5 29 4453.5 30 4762.0 31 5149.0 32 5841.0 33 6668.5 34 7411.0 35 7859.5 36 8053.0 37 8320.0 38 7822.5 39 7008.0 40 6775.5 41 6930.0 42 7547.5 43 8268.0 44 8825.0 45 8859.5 46 9224.0 47 13895.0 48 23484.5 49 22353.0 50 12528.0 51 7832.0 52 6070.5 53 5793.5 54 5563.0 55 5518.0 56 6140.5 57 6682.0 58 7335.0 59 8152.0 60 8750.5 61 8857.5 62 7441.5 63 4430.0 64 2203.0 65 1532.0 66 1112.5 67 836.0 68 633.5 69 463.0 70 356.5 71 319.0 72 300.0 73 302.0 74 246.0 75 183.5 76 134.5 77 63.5 78 33.5 79 17.5 80 9.0 81 6.0 82 4.0 83 3.5 84 2.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01654480594684183 2 5.805195069067308E-4 3 5.805195069067308E-4 4 0.0 5 0.0023220780276269233 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00478928593198053 14-15 0.002031818274173558 16-17 0.0 18-19 4.3538963018004807E-4 20-21 7.256493836334135E-4 22-23 0.0011610390138134617 24-25 0.001015909137086779 26-27 2.902597534533654E-4 28-29 0.0 30-31 0.0 32-33 1.451298767266827E-4 34-35 0.0 36-37 5.805195069067308E-4 38-39 0.008272402973420915 40-41 7.256493836334135E-4 42-43 0.0 44-45 7.256493836334135E-4 46-47 1.451298767266827E-4 48-49 0.0 50-51 8.707792603600961E-4 52-53 2.902597534533654E-4 54-55 0.0 56-57 2.902597534533654E-4 58-59 0.0 60-61 0.0011610390138134617 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.902597534533654E-4 72-73 1.451298767266827E-4 74-75 0.0023220780276269233 76-77 0.0 78-79 2.902597534533654E-4 80-81 0.001451298767266827 82-83 0.0 84-85 8.707792603600961E-4 86-87 1.451298767266827E-4 88-89 0.0017415585207201923 90-91 8.707792603600961E-4 92-93 0.0 94-95 0.0 96-97 1.451298767266827E-4 98-99 7.256493836334135E-4 100-101 0.0023220780276269233 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 344519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.15091156125509 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36958565936798 31.466103029014892 2 11.706631646741776 9.400638638570383 3 3.891151423778826 4.686998300627866 4 1.8230432487972732 2.9278739301920997 5 0.9176126795744638 1.8421492772540304 6 0.6184548740943432 1.4898916172633478 7 0.3887886223761345 1.0927152315132467 8 0.2896930309240731 0.9305151411635517 9 0.21020378811681148 0.7595886335867013 >10 1.4460711238674397 11.279261493219604 >50 0.16035306252475967 4.585709083122119 >100 0.15024070723040545 11.949466946109325 >500 0.014446221849077448 3.780338850145258 >1k 0.011556977479261958 8.626190928939053 >5k 0.002166933277361617 5.18255889927854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6153 1.7859682629985572 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6094 1.7688429375448087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5623 1.6321305936682735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3258 0.9456662767510645 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2846 0.8260792583282779 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2541 0.7375500335250016 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2511 0.7288422409214006 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2491 0.7230370458523333 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1959 0.5686188570151429 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1779 0.5163721013935371 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1657 0.4809604114722265 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1522 0.44177534475602215 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1493 0.4333578119058746 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1483 0.4304552143713409 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1391 0.40375131705363126 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1294 0.37559612096865486 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1231 0.3573097565010928 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1172 0.34018443104734425 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1116 0.3239298848539558 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 950 0.2757467657806972 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 844 0.24497923191464044 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 794 0.23046624424197215 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 788 0.22872468572125193 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 719 0.20869676273296972 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.20202078840354235 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 696 0.20202078840354235 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 694 0.2014402688966356 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 670 0.19447403481375483 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 668 0.1938935153068481 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 623 0.18083182640144665 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 588 0.17067273503057886 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 584 0.1695116960167654 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 573 0.16631883872877837 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 565 0.16399676070115146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 535 0.1552889680975505 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 532 0.15441818883719038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 510 0.14803247426121638 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 503 0.14600065598704282 No Hit GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC 503 0.14600065598704282 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 489 0.1419370194386957 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 471 0.1367123438765351 No Hit TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC 462 0.13410000609545483 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 456 0.13235844757473464 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 455 0.13206818782128127 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 450 0.13061688905401445 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 433 0.12568247324530724 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.11900649891587983 No Hit ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA 409 0.11871623916242646 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 406 0.11784545990206635 No Hit CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC 395 0.11465260261407935 No Hit GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 388 0.11262078433990577 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 381 0.11058896606573224 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 379 0.1100084465588255 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 379 0.1100084465588255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 369 0.10710584902429185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.902597534533654E-4 0.0 10-11 0.0 0.0 0.0 5.805195069067308E-4 0.0 12-13 0.0 0.0 0.0 5.805195069067308E-4 0.0 14-15 0.0 0.0 0.0 5.805195069067308E-4 0.0 16-17 0.0 0.0 0.0 5.805195069067308E-4 0.0 18-19 0.0 0.0 0.0 7.256493836334135E-4 0.0 20-21 0.0 0.0 0.0 0.001015909137086779 0.0 22-23 0.0 0.0 0.0 0.002467207904353606 0.0 24-25 0.0 0.0 0.0 0.005224675562160578 0.0 26-27 0.0 0.0 0.0 0.010594481001047838 0.0 28-29 0.0 0.0 0.0 0.020608442495188943 0.0 30-31 0.0 0.0 0.0 0.03845941733257092 0.0 32-33 0.0 0.0 0.0 0.05747143118376635 0.0 34-35 0.0 0.0 0.0 0.07459675663751492 0.0 36-37 0.0 0.0 0.0 0.09433441987234376 0.0 38-39 0.0 0.0 0.0 0.111604875202819 0.0 40-41 0.0 0.0 0.0 0.1268435122591207 0.0 42-43 0.0 0.0 0.0 0.1433883182059625 0.0 44-45 0.0 0.0 0.0 0.1577561760019041 0.0 46-47 0.0 0.0 0.0 0.1814123459083534 0.0 48-49 0.0 0.0 0.0 0.20666494445879618 0.0 50-51 0.0 0.0 0.0 0.2300308546117921 0.0 52-53 0.0 0.0 0.0 0.2697964408349032 0.0 54-55 0.0 0.0 0.0 0.3686298868857741 0.0 56-57 0.0 0.0 0.0 0.5324815177101988 0.0 58-59 0.0 0.0 0.0 0.6898023040819229 0.0 60-61 0.0 0.0 0.0 0.8359480899456924 0.0 62-63 0.0 0.0 0.0 1.0132967993056987 0.0 64-65 0.0 0.0 0.0 1.2154627175859676 0.0 66-67 0.0 0.0 0.0 1.5317007189734093 0.0 68-69 0.0 0.0 0.0 1.912811775257678 0.0 70-71 0.0 0.0 0.0 2.236741660111634 0.0 72-73 0.0 0.0 0.0 2.5677829089251976 0.0 74-75 0.0 0.0 0.0 2.923496236782296 0.0 76-77 0.0 0.0 0.0 3.301414435778578 0.0 78-79 0.0 0.0 0.0 3.620409904823827 0.0 80-81 0.0 0.0 0.0 3.942307971403609 0.0 82-83 0.0 0.0 0.0 4.295844351109809 0.0 84-85 0.0 0.0 0.0 4.8676560654129375 0.0 86-87 0.0 0.0 0.0 5.573567785811523 0.0 88-89 0.0 0.0 0.0 6.3125691181037915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 60 8.280898E-5 39.581516 7 GTTATCA 75 3.0669876E-4 31.679008 1 CGCTCCG 40 4.4853654E-4 29.694761 34-35 CATGGGC 305 0.0 29.588808 4 ACTGGGC 115 3.8556227E-6 28.911718 8 ATAGTCA 85 6.399718E-4 27.939896 8 CCGATAA 70 0.008385432 27.141615 9 CGTCACT 195 5.293259E-10 26.805315 1 AGATAGT 90 8.937174E-4 26.387678 6 CGACCGT 45 8.9462893E-4 26.387678 18-19 GTTCGAC 45 8.9462893E-4 26.387678 16-17 CGCAGAA 90 8.937174E-4 26.387678 9 TAGTCAA 90 8.937174E-4 26.387678 9 ATCGCGT 65 1.0017035E-5 25.57575 24-25 CATGGGT 245 1.0913936E-11 25.202927 4 GTCACTA 250 1.4551915E-11 24.69887 2 TCCGCCA 60 1.7070153E-4 23.755808 36-37 GCTCCGC 60 1.7070153E-4 23.755808 34-35 TTGCCGC 60 1.7086967E-4 23.752357 80-81 CTTTGCG 140 1.7754219E-5 23.748913 2 >>END_MODULE