##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937027_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 211833 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891480553077187 31.0 31.0 34.0 30.0 34.0 2 31.781955597097713 31.0 31.0 34.0 30.0 34.0 3 31.94556561064612 31.0 31.0 34.0 30.0 34.0 4 35.69069030793125 37.0 35.0 37.0 33.0 37.0 5 35.65268867456912 37.0 35.0 37.0 33.0 37.0 6 35.839397072221985 37.0 35.0 37.0 35.0 37.0 7 35.78336708633688 37.0 35.0 37.0 33.0 37.0 8 35.8344686616344 37.0 35.0 37.0 35.0 37.0 9 37.362847148461285 39.0 37.0 39.0 34.0 39.0 10-11 37.29794224695869 39.0 37.0 39.0 33.5 39.0 12-13 37.208789470951174 39.0 37.0 39.0 33.0 39.0 14-15 37.88681886202811 39.5 37.0 41.0 33.0 41.0 16-17 37.95965925988869 39.0 37.0 41.0 33.0 41.0 18-19 38.19984846553653 40.0 37.0 41.0 33.5 41.0 20-21 38.323844726742294 40.0 37.0 41.0 34.0 41.0 22-23 38.28078486354817 40.0 37.0 41.0 34.0 41.0 24-25 38.05275145987642 40.0 37.0 41.0 33.5 41.0 26-27 37.58061539042548 39.5 36.0 41.0 32.5 41.0 28-29 37.377693749321395 39.0 36.0 41.0 33.0 41.0 30-31 37.18429848040674 39.0 35.0 40.5 33.0 41.0 32-33 36.913873192562065 38.5 35.0 41.0 33.0 41.0 34-35 36.65948412192623 38.0 35.0 40.5 32.0 41.0 36-37 36.26560073265261 38.0 35.0 40.0 31.0 41.0 38-39 35.90283619643776 38.0 35.0 40.0 30.5 41.0 40-41 35.63650847601649 38.0 35.0 40.0 30.0 41.0 42-43 35.363732279673144 38.0 35.0 40.0 30.0 41.0 44-45 34.872111521811995 38.0 35.0 40.0 26.0 41.0 46-47 34.31935770158569 38.0 34.0 40.0 23.5 41.0 48-49 33.72963844160259 38.0 33.0 40.0 20.5 41.0 50-51 33.321923401925105 37.0 33.0 40.0 18.5 41.0 52-53 32.93351366406556 37.0 33.0 40.0 13.5 41.0 54-55 32.78834978497213 37.0 33.0 40.0 9.0 41.0 56-57 32.48519352508816 36.5 32.5 40.0 4.5 41.0 58-59 32.1903409761463 36.0 32.0 40.0 2.0 41.0 60-61 31.574688551830924 35.0 31.0 39.0 2.0 40.5 62-63 31.141514778150714 35.0 31.0 39.0 2.0 40.0 64-65 30.53102207871295 35.0 30.0 38.0 2.0 40.0 66-67 30.06902135172518 35.0 29.5 37.0 2.0 39.0 68-69 29.58787582671255 35.0 29.0 37.0 2.0 39.0 70-71 28.91145383391634 34.0 28.5 36.0 2.0 38.5 72-73 28.500394178433012 34.0 28.0 35.0 2.0 37.0 74-75 28.090472211600648 34.0 27.0 35.0 2.0 37.0 76-77 26.647946731623495 31.5 26.0 34.0 2.0 35.5 78-79 27.319978473608927 33.0 26.0 35.0 2.0 36.0 80-81 27.15924336623661 33.0 26.0 35.0 2.0 36.0 82-83 26.807402529350952 33.0 25.5 35.0 2.0 35.0 84-85 26.458922830720425 33.0 25.0 35.0 2.0 35.0 86-87 26.09650998664042 33.0 24.0 35.0 2.0 35.0 88-89 25.54948237526731 32.5 21.5 35.0 2.0 35.0 90-91 24.90531928453074 32.0 18.5 34.5 2.0 35.0 92-93 24.55823691303999 32.0 14.0 34.0 2.0 35.0 94-95 24.053759329282975 32.0 4.0 34.0 2.0 35.0 96-97 23.227535369843224 31.0 2.0 34.0 2.0 35.0 98-99 22.53853035173934 31.0 2.0 34.0 2.0 35.0 100-101 19.94145152077344 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2982719743395883 1101 2 0.3126509602063159 1101 3 0.3410829116964962 1101 4 0.27526427546082743 1101 5 0.17270038464535986 1101 6 -0.009178638468121392 1101 7 0.14964405198892194 1101 8 0.054867026369301186 1101 9 0.28246300481039555 1101 10-11 0.1705670113201876 1101 12-13 0.1567332448823251 1101 14-15 0.5160001403534622 1101 16-17 0.2816850734476688 1101 18-19 0.26738093169230126 1101 20-21 0.3056896207618891 1101 22-23 0.3744396017807219 1101 24-25 0.30985495071875135 1101 26-27 0.22616781329153923 1101 28-29 0.3060336833860333 1101 30-31 0.6989113397531312 1101 32-33 0.7378174727289064 1101 34-35 0.902016987674763 1101 36-37 0.9880955603973902 1101 38-39 1.1135683213129752 1101 40-41 0.8226468238254512 1101 42-43 0.9837189216360613 1101 44-45 1.2086576695414735 1101 46-47 1.1595925350260146 1101 48-49 1.2632985968323638 1101 50-51 1.4258498096717815 1101 52-53 1.166262902392262 1101 54-55 1.5078807008728674 1101 56-57 1.7295012684881286 1101 58-59 1.9162989228071332 1101 60-61 1.7359826378843444 1101 62-63 1.8474249552011166 1101 64-65 2.3935302566091643 1101 66-67 2.2931099242194684 1101 68-69 2.41521650158316 1101 70-71 2.350458638225952 1101 72-73 2.8332732226370965 1101 74-75 2.657705371726795 1101 76-77 2.310277955042018 1101 78-79 2.4118817172336406 1101 80-81 2.7085356135080545 1101 82-83 2.9177434692470143 1101 84-85 2.737797606612485 1101 86-87 2.719889985333097 1101 88-89 2.904921881997087 1101 90-91 2.5727680945843865 1101 92-93 2.5847730154937913 1101 94-95 3.004734020259722 1101 96-97 2.429495940559484 1101 98-99 2.4360394971303094 1101 100-101 1.882633911299994 1102 1 0.0626649387325493 1102 2 -0.018708971153618137 1102 3 0.048247218860804963 1102 4 0.3284603286568739 1102 5 0.02272183466681099 1102 6 -0.021619650909137533 1102 7 0.09353079587566526 1102 8 0.1773037488060254 1102 9 0.29327381562120536 1102 10-11 0.4632740040271841 1102 12-13 0.25218584033491 1102 14-15 0.5288701532234796 1102 16-17 0.5363397281023197 1102 18-19 0.5986121629235299 1102 20-21 0.5568264718987379 1102 22-23 0.6032684306095533 1102 24-25 0.42937447023827247 1102 26-27 0.7302862174099474 1102 28-29 0.5653215426738925 1102 30-31 0.7813652222070075 1102 32-33 0.966474701386133 1102 34-35 0.6895759752337511 1102 36-37 0.9637712360730717 1102 38-39 0.9063611141057635 1102 40-41 1.1182710194496508 1102 42-43 0.8097163476334899 1102 44-45 1.1424629033467042 1102 46-47 1.098159673593159 1102 48-49 1.1496992832330406 1102 50-51 1.137261221083186 1102 52-53 1.1658339019632606 1102 54-55 1.0008879938801627 1102 56-57 1.1341773731642277 1102 58-59 1.404244010752219 1102 60-61 1.3390285409302471 1102 62-63 1.5989050066811679 1102 64-65 1.5220587851376948 1102 66-67 1.497957629067173 1102 68-69 1.582397968764628 1102 70-71 1.7906559784232954 1102 72-73 2.1665207558846333 1102 74-75 2.054745268766691 1102 76-77 1.8868545316185923 1102 78-79 1.9112811166330381 1102 80-81 1.9255240304964722 1102 82-83 2.1075332590368028 1102 84-85 2.0854166542315333 1102 86-87 1.5288989943421072 1102 88-89 2.08540236247757 1102 90-91 2.585080406896701 1102 92-93 2.111800042520816 1102 94-95 2.1913921069178066 1102 96-97 2.7040991151626628 1102 98-99 2.7046366657274774 1102 100-101 2.908030736696819 1103 1 -0.09561170525838136 1103 2 -0.3658745040334388 1103 3 -0.04563033215960388 1103 4 -0.1284557711163643 1103 5 -0.021269095038405794 1103 6 0.28563658491852806 1103 7 0.2971589137895023 1103 8 0.1559885560622618 1103 9 0.07399943920397334 1103 10-11 0.09694747341493581 1103 12-13 0.13370511471133284 1103 14-15 -0.061265901198289896 1103 16-17 -0.012072970310377684 1103 18-19 0.1652788295901999 1103 20-21 0.11827771906427387 1103 22-23 0.28093282970252176 1103 24-25 0.060666987245070914 1103 26-27 0.2379959679768433 1103 28-29 0.27019682612061047 1103 30-31 0.3387348367194818 1103 32-33 0.2629599621571117 1103 34-35 -0.0939841154691976 1103 36-37 -0.11457343512874019 1103 38-39 0.22325453814205076 1103 40-41 0.37314630289635886 1103 42-43 0.4618705426448244 1103 44-45 0.30187333418570717 1103 46-47 0.494304798309706 1103 48-49 0.11013012223531149 1103 50-51 0.15018639115121601 1103 52-53 0.44814682713333553 1103 54-55 0.728325635603511 1103 56-57 0.6283950602390505 1103 58-59 0.29846169782704735 1103 60-61 0.4009333028350106 1103 62-63 0.4584288162049752 1103 64-65 0.3959816876320268 1103 66-67 0.5058941370036827 1103 68-69 0.5246882181977313 1103 70-71 0.16968092173394922 1103 72-73 0.27581780803882694 1103 74-75 0.6658563798778019 1103 76-77 0.38628763819455614 1103 78-79 0.5612811166330367 1103 80-81 0.6754106518116636 1103 82-83 0.5629754359075498 1103 84-85 0.6493622324628276 1103 86-87 0.07583776985231339 1103 88-89 0.5577379633846 1103 90-91 0.9338332413638213 1103 92-93 0.90601772959565 1103 94-95 0.8170156896842435 1103 96-97 1.0043258725322772 1103 98-99 1.3512353051832626 1103 100-101 1.7273051131140527 1104 1 0.2309189069865205 1104 2 0.008275155830506975 1104 3 0.1641202347338222 1104 4 0.1975079477044943 1104 5 0.1465313584763308 1104 6 0.12520574591626144 1104 7 0.266546668891543 1104 8 0.388414859917134 1104 9 0.29168651403390555 1104 10-11 0.2604962262494013 1104 12-13 0.16488425303332832 1104 14-15 0.30267967703300513 1104 16-17 0.47800639476898965 1104 18-19 0.36964390895527544 1104 20-21 0.21238202745429646 1104 22-23 0.34414144648255984 1104 24-25 0.5099300257938211 1104 26-27 0.41004812217185815 1104 28-29 0.3994485268008816 1104 30-31 0.5516033174451067 1104 32-33 0.36131597122741255 1104 34-35 0.4788616895194622 1104 36-37 0.7193267916286246 1104 38-39 0.7337420664867196 1104 40-41 0.9547789559575861 1104 42-43 0.8819385698557127 1104 44-45 1.3095263954101952 1104 46-47 1.2763342767677628 1104 48-49 1.4973183308520888 1104 50-51 1.388848522670493 1104 52-53 1.1836910448203994 1104 54-55 1.0568403748325395 1104 56-57 1.2607646747515346 1104 58-59 1.8455138520220622 1104 60-61 1.8672031441048524 1104 62-63 2.05366691144307 1104 64-65 2.021265134344045 1104 66-67 2.107084613194157 1104 68-69 1.582794794161451 1104 70-71 1.8354972482645628 1104 72-73 1.8605683749322495 1104 74-75 2.1837135227349442 1104 76-77 1.902727547491608 1104 78-79 1.745804926156847 1104 80-81 2.0719526019250445 1104 82-83 2.203565005068551 1104 84-85 2.370734114548995 1104 86-87 2.1038989943421065 1104 88-89 2.718735695810903 1104 90-91 2.740239137055429 1104 92-93 2.572117502838278 1104 94-95 2.1687730592987613 1104 96-97 2.7548927659563134 1104 98-99 2.7951128562036693 1104 100-101 2.2973164509825317 1105 1 -0.11013643406882423 1105 2 -0.29996165240629935 1105 3 0.08132315193673634 1105 4 0.21820721840376933 1105 5 0.13666434860932242 1105 6 0.0673979381084564 1105 7 0.013007415352291218 1105 8 0.1584706299729035 1105 9 -0.07189134954396081 1105 10-11 0.0654619062150843 1105 12-13 -0.003176665027588399 1105 14-15 0.2592433835967114 1105 16-17 -0.03363024186764818 1105 18-19 0.04816171247307466 1105 20-21 0.2441280592003281 1105 22-23 0.493058220399341 1105 24-25 0.3068519477157423 1105 26-27 0.37331496043869095 1105 28-29 0.7820096593620107 1105 30-31 0.5367491775909699 1105 32-33 0.580159815071255 1105 34-35 0.7578728435306203 1105 36-37 0.82217964448148 1105 38-39 0.6233280810727351 1105 40-41 0.48856273974137565 1105 42-43 0.7802654681826127 1105 44-45 1.3798288407126407 1105 46-47 1.4906736161070953 1105 48-49 0.9787640622978202 1105 50-51 0.9791531129750766 1105 52-53 0.7060526921820482 1105 54-55 0.45833115132331415 1105 56-57 0.5320538210406767 1105 58-59 0.7593920159002288 1105 60-61 0.825321977223684 1105 62-63 0.16048801826417858 1105 64-65 0.3559927190716259 1105 66-67 0.2645813956909393 1105 68-69 0.4414927778594375 1105 70-71 0.8707289084962255 1105 72-73 0.8490389884028673 1105 74-75 0.8161137801352005 1105 76-77 0.6428605376245997 1105 78-79 0.40812796347988467 1105 80-81 0.16424131921376173 1105 82-83 0.5701458716494194 1105 84-85 0.782842651657532 1105 86-87 0.5847548501979638 1105 88-89 0.7149819420571468 1105 90-91 1.0937891156054071 1105 92-93 1.3077459884667633 1105 94-95 1.311040326566026 1105 96-97 1.7087752198387633 1105 98-99 1.5711746322654427 1105 100-101 1.814315592981675 1106 1 0.14139509746270917 1106 2 -0.0907724632171103 1106 3 -0.023181352567767988 1106 4 0.31100001119656184 1106 5 0.09288056482554197 1106 6 0.13298352369403688 1106 7 0.22273714508201437 1106 8 -0.04333117182889623 1106 9 0.1088293711767605 1106 10-11 0.08708352783671103 1106 12-13 0.321868380017456 1106 14-15 0.2774415817949105 1106 16-17 0.05745083921343763 1106 18-19 -0.11472117040980834 1106 20-21 -0.06587194079967418 1106 22-23 0.02215731949844013 1106 24-25 0.006041136904933353 1106 26-27 0.07520685233058089 1106 28-29 0.16849614584849348 1106 30-31 0.13485728569906996 1106 32-33 0.055204860116297993 1106 34-35 0.17751248317026125 1106 36-37 0.24488234718418056 1106 38-39 0.607472225216874 1106 40-41 -0.18522104404241446 1106 42-43 -0.15568047776333316 1106 44-45 0.06595496683876689 1106 46-47 -0.26977683434335376 1106 48-49 -0.40204674851298705 1106 50-51 -0.3494054455834785 1106 52-53 -0.4051184789891167 1106 54-55 0.015808628800790814 1106 56-57 -0.04677500778815613 1106 58-59 -0.3705178940096836 1106 60-61 0.10045711235882138 1106 62-63 0.6431907209668815 1106 64-65 0.6342810073599132 1106 66-67 0.4016084227179668 1106 68-69 0.011763048129708409 1106 70-71 0.07144962921694287 1106 72-73 0.6825525019163798 1106 74-75 0.49061828463970514 1106 76-77 0.3157434205074807 1106 78-79 0.5812811166330398 1106 80-81 0.25568276065520124 1106 82-83 0.38419992570347006 1106 84-85 0.6477976066124889 1106 86-87 0.03223232767544104 1106 88-89 0.14318014025534964 1106 90-91 0.7973026291189242 1106 92-93 1.4618000425208173 1106 94-95 0.682661948187647 1106 96-97 0.8261626072261521 1106 98-99 1.5589223800131933 1106 100-101 1.3648561335222134 1107 1 0.2414359972582112 1107 2 0.13667129956407464 1107 3 0.10749349405799791 1107 4 0.22163395802682828 1107 5 0.3038008102243097 1107 6 0.10390376909280974 1107 7 0.14457763587956407 1107 8 0.3679162719338862 1107 9 0.311406058293322 1107 10-11 0.2484332210882414 1107 12-13 0.1108867849254338 1107 14-15 0.4308567147192477 1107 16-17 0.9002115754097488 1107 18-19 0.4914546987108537 1107 20-21 0.047809040795421254 1107 22-23 -0.032587056779682655 1107 24-25 0.36107181175155745 1107 26-27 0.23555450059234317 1107 28-29 0.515939908629683 1107 30-31 0.49557303212034043 1107 32-33 0.39514351042304696 1107 34-35 0.2932180046426538 1107 36-37 0.5018762122148601 1107 38-39 0.7669609777731097 1107 40-41 0.6234906123993014 1107 42-43 0.9289002993327813 1107 44-45 1.0897995476158613 1107 46-47 1.5448243926505185 1107 48-49 1.871940981548363 1107 50-51 1.8157888284451573 1107 52-53 1.9075809075139532 1107 54-55 1.3805939048744094 1107 56-57 1.691466301005299 1107 58-59 1.0135925354381676 1107 60-61 0.57242030254287 1107 62-63 0.6837428682061422 1107 64-65 0.8999047292413067 1107 66-67 1.383632962595268 1107 68-69 1.1417221483342033 1107 70-71 0.9283616946566156 1107 72-73 0.6422048536546185 1107 74-75 0.7352399615313203 1107 76-77 0.7972158131455203 1107 78-79 1.3900132229725095 1107 80-81 1.650161288262563 1107 82-83 1.4989443019815916 1107 84-85 1.5435235779826293 1107 86-87 1.7391239432173649 1107 88-89 2.1130778907665935 1107 90-91 2.4600633653152393 1107 92-93 2.2304094494737825 1107 94-95 2.0338889420526804 1107 96-97 2.5358354088621446 1107 98-99 2.681908065084766 1107 100-101 1.880929753154117 1108 1 -0.019282868638985207 1108 2 0.013521322093623667 1108 3 -0.3412604486129638 1108 4 -0.09069490405767766 1108 5 -0.06519853121965724 1108 6 0.15298352369404 1108 7 0.20318912248314547 1108 8 0.08265752873607823 1108 9 0.15730394744794296 1108 10-11 0.257704996763259 1108 12-13 -0.04146495331587374 1108 14-15 -0.01995954814859857 1108 16-17 -0.16441356756622838 1108 18-19 0.23025058100262186 1108 20-21 0.24130320044326226 1108 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90-91 -0.5493011444659821 2316 92-93 -0.38128171848547154 2316 94-95 -0.7692248442651817 2316 96-97 -0.9183028015788786 2316 98-99 -0.7012034061503307 2316 100-101 -1.1045778287419346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 26.0 6 49.0 7 83.0 8 126.0 9 274.0 10 502.0 11 820.0 12 1082.0 13 1375.0 14 1709.0 15 2391.0 16 3254.0 17 4277.0 18 3763.0 19 2439.0 20 2292.0 21 2167.0 22 2238.0 23 2396.0 24 2753.0 25 2869.0 26 3204.0 27 3800.0 28 4592.0 29 5505.0 30 6530.0 31 7955.0 32 9838.0 33 12479.0 34 17271.0 35 26112.0 36 31155.0 37 31344.0 38 14367.0 39 790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.47142364766928 19.16327118379989 15.724666078063843 12.640639090466996 2 13.84526051919727 29.32723401643794 35.436464662200756 21.391040802164028 3 27.16462018533992 32.047850368461056 29.953216539912287 10.834312906286735 4 10.662172560460363 18.42583544584649 40.50077183441673 30.411220159276407 5 10.270548414050957 45.56033404302527 30.269226593148247 13.899890949775525 6 22.897242587181168 41.0836098928853 16.458558554305597 19.560588965627936 7 22.689571502079467 41.97835087073308 19.10656035650725 16.225517270680207 8 24.20680441668673 28.158030146388903 19.620644564350222 28.01452087257415 9 24.9394570251094 25.193430674163135 22.72828124041108 27.13883106031638 10-11 23.87221949469391 28.02102609615167 24.209751123531856 23.89700328562257 12-13 34.44183494034201 28.12741198824543 22.387499557433348 15.043253513979204 14-15 26.3639281887147 28.493671901922742 32.007052725496024 13.135347183866536 16-17 15.950536507531876 31.9690510921339 31.890451440521545 20.189960959812684 18-19 15.833463152577737 36.053636591088264 33.49029660156822 14.622603654765781 20-21 20.609319419430523 28.365903227405447 31.115559195851322 19.909218157312704 22-23 17.445818168085236 30.339937592348694 30.965194280400127 21.249049959165948 24-25 21.818276234852025 30.37573113908992 31.84650681924401 15.95948580681405 26-27 22.081594501199085 26.23236777007761 37.0555356232415 14.630502105481805 28-29 16.325466008577816 32.335342062216306 35.924853240049 15.414338689156876 30-31 17.37805381849772 33.975670960720855 32.428489479685275 16.21778574109615 32-33 17.06710286241659 33.516497782435565 31.809716685195543 17.6066826699523 34-35 19.387991143716334 29.922059038743882 32.42480892426367 18.265140893276115 36-37 20.501115294284265 30.025610461342367 35.12480674133434 14.34846750303903 38-39 21.05986182283393 29.545695948864537 34.99394560247747 14.400496625824072 40-41 15.784057084992527 32.70041945100779 35.62005339325716 15.895470070742526 42-43 15.05247156904862 36.05785798922716 30.09101681072941 18.798653630994814 44-45 18.199799841383737 32.51255712073719 29.714585142943466 19.57305789493561 46-47 19.432524677458186 33.15441881104455 28.104450203698196 19.308606307799067 48-49 20.561577098833038 32.209486763095285 29.598455379734883 17.630480758336795 50-51 19.277685724131747 33.54387654425892 32.89761274211289 14.280824989496443 52-53 17.747166373193725 36.01762725946627 29.956427529492853 16.278778837847153 54-55 13.752292560184868 33.23073364286338 31.776713489577556 21.240260307374196 56-57 19.360861437365458 34.11359001472865 27.617640394274705 18.90790815363118 58-59 21.188739056646973 31.76776605713557 27.686653653055878 19.356841233161575 60-61 17.720730204738683 31.76659695700818 32.213887485778756 18.29878535247438 62-63 20.556759334003672 31.08415591527288 30.44473712783183 17.91434762289162 64-65 18.583978889030508 35.05143202428328 30.433879518299793 15.930709568386417 66-67 17.130947491656162 31.343086299112983 30.191471583747575 21.334494625483284 68-69 15.937790618081223 35.60210165554942 29.07101348703932 19.38909423933004 70-71 20.01387885740182 33.83325544178669 26.28202404724476 19.870841653566725 72-73 18.164823622437538 31.016015011860787 29.841030059126904 20.97813130657477 74-75 17.29692091628999 35.96851285803642 28.075012096821784 18.659554128851806 76-77 19.401124956037965 30.85863461588393 28.84759276874671 20.8926476593314 78-79 17.128708430993502 31.445276080280788 30.625992829206233 20.800022659519477 80-81 18.26862247065822 34.371901762867424 28.062356595881294 19.297119170593067 82-83 17.25953027263071 31.820370588929542 29.141508320547622 21.778590817892127 84-85 16.832332884055678 31.345023994259318 28.904053648944984 22.91858947274002 86-87 17.793435541550117 32.69954797058858 28.45967732417472 21.047339163686576 88-89 18.414598740006564 30.865826513679828 27.365320203090736 23.354254543222876 90-91 16.40332905321198 29.501444540956985 30.037954605536466 24.057271800294572 92-93 17.191885097895742 33.80430292802096 27.866828744408906 21.13698322967439 94-95 17.422491827269187 33.2994228930877 26.56131613420981 22.716769145433304 96-97 13.91563378519685 32.205838268583015 28.747583517642976 25.130944428577163 98-99 17.64544812918546 33.39022139132611 25.468146228274545 23.496184251213887 100-101 17.197605581939897 31.972704011783254 26.18328250545259 24.64640790082426 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3980.0 1 2531.5 2 1122.0 3 1105.0 4 939.5 5 961.0 6 1534.0 7 2663.0 8 3524.0 9 3508.5 10 2839.5 11 2254.0 12 2106.5 13 1926.0 14 1696.0 15 1458.0 16 1244.0 17 1097.0 18 988.5 19 958.0 20 972.0 21 1018.5 22 1051.5 23 1051.5 24 1114.5 25 1264.5 26 1394.0 27 1566.0 28 1745.5 29 1876.0 30 1988.0 31 2092.5 32 2296.0 33 2496.5 34 2814.5 35 2982.5 36 3132.0 37 3308.5 38 3238.0 39 3033.0 40 2708.5 41 2510.0 42 2370.5 43 2371.5 44 2547.5 45 2837.0 46 4531.5 47 11869.0 48 35869.0 49 34520.5 50 11237.5 51 5184.0 52 2248.0 53 1469.5 54 1245.5 55 1147.0 56 1279.0 57 1329.0 58 1232.0 59 1263.0 60 1290.0 61 1269.5 62 1046.5 63 584.5 64 267.0 65 179.5 66 137.5 67 106.5 68 78.5 69 46.0 70 39.5 71 28.5 72 20.5 73 25.0 74 16.0 75 11.0 76 12.5 77 6.5 78 3.5 79 4.5 80 5.0 81 2.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015106239348921085 2 0.0028324198779227034 3 0.0028324198779227034 4 0.0 5 0.0018882799186151357 6 0.0018882799186151357 7 0.0 8 0.0 9 0.0 10-11 4.720699796537839E-4 12-13 2.3603498982689196E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0030684548677495952 22-23 0.0 24-25 0.0028324198779227034 26-27 0.002360349898268919 28-29 0.003540524847403379 30-31 0.01345399442013284 32-33 7.081049694806758E-4 34-35 0.0028324198779227034 36-37 0.002596384888095811 38-39 0.0016522449287882436 40-41 0.004012594827057163 42-43 0.0018882799186151357 44-45 4.720699796537839E-4 46-47 0.0 48-49 4.720699796537839E-4 50-51 0.0 52-53 9.441399593075678E-4 54-55 0.0030684548677495952 56-57 4.720699796537839E-4 58-59 0.0016522449287882436 60-61 9.441399593075678E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.3603498982689196E-4 74-75 2.3603498982689196E-4 76-77 0.0011801749491344596 78-79 7.081049694806758E-4 80-81 0.008969329613421893 82-83 0.0037765598372302713 84-85 0.005428804766018515 86-87 0.00495673478636473 88-89 0.0030684548677495952 90-91 4.720699796537839E-4 92-93 2.3603498982689196E-4 94-95 2.3603498982689196E-4 96-97 0.0030684548677495952 98-99 0.006372944725326082 100-101 0.003304489857576487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 211833.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.014053523294294 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2779688879418 25.20759277355275 2 10.262108435568814 6.365391605651621 3 3.331912691406131 3.100083556386399 4 1.4307893695394076 1.7749831234982274 5 0.855429389022497 1.3265166428271327 6 0.5114310937928095 0.9516930789820284 7 0.36835215683886874 0.7996865455335098 8 0.26484824499984777 0.6571214116780671 9 0.1933087765228774 0.539575986744275 >10 1.211604615056775 7.02015266743142 >50 0.11415872629303783 2.414165875949451 >100 0.10654814454016866 6.801112196872064 >500 0.025875977959755242 5.554375380606421 >1k 0.041097141465493627 27.70767538579919 >5k 0.004566349051721513 9.779873768487441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8206 3.8738062530389508 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7142 3.371523794687324 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5369 2.5345437207611656 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4882 2.3046456406697726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4461 2.10590417923553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3533 1.6678232381168185 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3458 1.6324179896427846 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3352 1.5823785717994836 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3344 1.5786020119622532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 1.3213238730509411 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2522 1.190560488686843 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2362 1.1150292919422375 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2092 0.9875703974357158 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1953 0.92195267026384 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1893 0.893628471484613 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1888 0.891268121586344 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1751 0.8265945343737755 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1680 0.793077565818357 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1673 0.7897730759607804 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1661 0.784108236204935 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1643 0.775610976571167 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1628 0.7685299268763601 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1506 0.7109373893585985 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1436 0.6778924907828336 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1379 0.650984501942568 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1330 0.6278530729395325 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1322 0.6240765131023023 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1101 0.519749047598816 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1041 0.49142484881958903 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1004 0.473958259572399 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 953 0.44988269061005604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.4357205912204425 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 827 0.39040187317367925 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 787 0.3715190739875279 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 771 0.3639659543130674 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 704 0.3323372656762638 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 703 0.33186519569661005 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 698 0.3295048457983411 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.3252562159814571 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 686 0.32384000604249574 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.3153427464087276 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 666 0.31439860644942 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 560 0.26435918860611896 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 551 0.26011055878923495 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 543 0.25633399895200465 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 532 0.25114122917581305 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 505 0.23839533972516086 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 487 0.22989808009139276 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 460 0.21715219064074057 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 451 0.21290356082385656 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 420 0.19826939145458924 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 419 0.19779732147493545 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 400 0.18882799186151356 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 392 0.18505143202428329 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 358 0.16900105271605464 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 350 0.16522449287882435 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 350 0.16522449287882435 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 332 0.15672723324505625 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 318 0.15011825352990327 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 315 0.1487020435909419 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 311 0.1468137636723268 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 310 0.146341693692673 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 309 0.14586962371301923 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 298 0.1406768539368276 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 289 0.13642822411994354 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 287 0.13548408416063595 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.128875104445483 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 260 0.1227381947099838 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 241 0.11376886509656191 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 233 0.10999230525933165 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 217 0.10243918558487111 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.7206997965378386E-4 0.0 14-15 0.0 0.0 0.0 7.081049694806758E-4 0.0 16-17 0.0 0.0 0.0 0.0018882799186151354 0.0 18-19 0.0 0.0 0.0 0.0025963848880958115 0.0 20-21 0.0 0.0 0.0 0.004720699796537838 0.0 22-23 0.0 0.0 0.0 0.006608979715152974 0.0 24-25 0.0 0.0 0.0 0.011329679511690813 0.0 26-27 0.0 0.0 0.0 0.019118834175978247 0.0 28-29 0.0 0.0 0.0 0.038001633362129604 0.0 30-31 0.0 0.0 0.0 0.06184116733464569 0.0 32-33 0.0 0.0 0.0 0.08025189654114326 0.0 34-35 0.0 0.0 0.0 0.09606624085954502 0.0 36-37 0.0 0.0 0.0 0.11612921499483084 0.0 38-39 0.0 0.0 0.0 0.1428011688452696 0.0 40-41 0.0 0.0 0.0 0.20369819622060775 0.0 42-43 0.0 0.0 0.0 0.3186472362663041 0.0 44-45 0.0 0.0 0.0 0.40409190258363903 0.0 46-47 0.0 0.0 0.0 0.6250206530616098 0.0 48-49 0.0 0.0 0.0 0.8209296946179301 0.0 50-51 0.0 0.0 0.0 0.928325614989166 0.0 52-53 0.0 0.0 0.0 1.063809699149802 0.0 54-55 0.0 0.0 0.0 1.1910325586664967 0.0 56-57 0.0 0.0 0.0 1.476634896357036 0.0 58-59 0.0 0.0 0.0 1.8658565945815808 0.0 60-61 0.0 0.0 0.0 2.3280131046626353 0.0 62-63 0.0 0.0 0.0 2.7177068728668337 0.0 64-65 0.0 0.0 0.0 3.082144897159555 0.0 66-67 0.0 0.0 0.0 3.5853714954704885 0.0 68-69 0.0 0.0 0.0 4.11456194266238 0.0 70-71 0.0 0.0 0.0 4.489857576487138 0.0 72-73 0.0 0.0 0.0 4.876718924813414 0.0 74-75 0.0 0.0 0.0 5.32730972039295 0.0 76-77 0.0 0.0 0.0 5.715351243668361 0.0 78-79 0.0 0.0 0.0 6.1088215717097905 0.0 80-81 0.0 0.0 0.0 6.692536101551694 0.0 82-83 0.0 0.0 0.0 7.300798270335594 0.0 84-85 0.0 0.0 0.0 8.446512110955329 0.0 86-87 0.0 0.0 0.0 9.533689274097991 0.0 88-89 0.0 0.0 0.0 10.443368124890833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACCT 30 1.3630215E-6 79.171196 5 TCACTAC 45 1.5112231E-5 52.780796 3 CGTCACT 45 1.5112231E-5 52.780796 1 GTCACTA 50 2.818173E-5 47.502716 2 GTAAGCG 25 2.8229928E-5 47.502716 94-95 CACTACC 60 8.2650586E-5 39.585598 4 TACCTCC 65 1.3259241E-4 36.531925 7 CATGGGT 115 1.02909326E-7 33.04537 4 ATGGGGG 165 7.4578566E-11 31.668476 5 ATGGGCA 75 3.0690062E-4 31.668476 5 CTACCTC 75 3.0690062E-4 31.668476 6 TGGGGGA 80 4.4799066E-4 29.689196 6 ATGGGTG 65 0.0058318228 29.23244 5 GGGAGCT 65 0.0058385576 29.225542 7 GGGATGT 65 0.0058385576 29.225542 7 CTTTGCG 100 4.9323833E-5 28.501629 2 GAGTACT 2305 0.0 26.166685 12-13 TTTGCGT 110 9.437212E-5 25.910574 3 GCTTTGC 110 9.437212E-5 25.910574 1 TGGGAGC 130 9.966378E-6 25.578386 6 >>END_MODULE