##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158270 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.050015795792 33.0 31.0 34.0 31.0 34.0 2 32.0609148922727 33.0 31.0 34.0 30.0 34.0 3 32.01972578505086 33.0 31.0 34.0 30.0 34.0 4 35.591552410437856 37.0 35.0 37.0 33.0 37.0 5 35.60365198711063 37.0 35.0 37.0 33.0 37.0 6 35.77646426991849 37.0 35.0 37.0 35.0 37.0 7 35.76419409869211 37.0 35.0 37.0 35.0 37.0 8 35.746742907689395 37.0 35.0 37.0 35.0 37.0 9 37.348714222531115 39.0 37.0 39.0 35.0 39.0 10-11 37.17392114740633 39.0 37.0 39.0 34.0 39.0 12-13 37.14623744234536 39.0 37.0 39.0 34.0 39.0 14-15 37.9671036835787 40.0 37.5 41.0 33.0 41.0 16-17 37.99007076514816 40.0 37.5 41.0 33.5 41.0 18-19 38.12432236052315 40.0 37.5 41.0 34.0 41.0 20-21 38.118869653124406 40.0 37.5 41.0 34.0 41.0 22-23 37.98487710873823 40.0 37.0 41.0 34.0 41.0 24-25 37.716190686801035 40.0 36.5 41.0 33.5 41.0 26-27 37.51383079547608 40.0 36.5 41.0 33.0 41.0 28-29 37.336137612939915 39.5 36.0 41.0 33.0 41.0 30-31 36.94018133569217 39.0 35.5 41.0 33.0 41.0 32-33 36.40912364945979 38.0 35.0 41.0 32.0 41.0 34-35 36.08349339735894 38.0 35.0 40.0 31.0 41.0 36-37 35.64519807923169 38.0 35.0 40.0 30.5 41.0 38-39 35.262396537562395 38.0 35.0 40.0 30.0 41.0 40-41 34.928808365451445 37.5 35.0 40.0 29.0 41.0 42-43 34.58996019460416 37.5 35.0 40.0 26.5 41.0 44-45 34.09295191760915 37.5 35.0 40.0 23.5 41.0 46-47 33.27484362165919 37.0 33.5 40.0 13.0 41.0 48-49 32.599418714854366 37.0 33.0 40.0 2.0 41.0 50-51 31.640857395589816 36.0 32.0 39.0 2.0 40.5 52-53 31.707408226448475 36.5 32.5 39.5 2.0 40.0 54-55 32.02002906425729 37.0 33.0 40.0 2.0 41.0 56-57 31.79911543564794 36.5 33.0 40.0 2.0 41.0 58-59 31.546600745561385 36.0 32.5 40.0 2.0 41.0 60-61 31.042411701522717 35.5 31.5 39.5 2.0 41.0 62-63 30.654394389334684 35.0 31.0 39.0 2.0 41.0 64-65 30.13290895305491 35.0 30.0 39.0 2.0 40.5 66-67 29.58062172237316 35.0 29.0 38.5 2.0 40.0 68-69 29.184905541163836 35.0 29.0 37.5 2.0 39.0 70-71 28.517157389271496 35.0 27.0 37.0 2.0 39.0 72-73 27.956978580906046 35.0 26.0 36.0 2.0 38.5 74-75 27.52697921273773 34.5 26.0 35.5 2.0 37.0 76-77 27.014149870474505 34.0 25.5 35.0 2.0 37.0 78-79 26.519207683073226 34.0 23.0 35.0 2.0 36.0 80-81 26.034188412206987 34.0 20.5 35.0 2.0 36.0 82-83 25.63216023251406 33.5 19.0 35.0 2.0 35.5 84-85 25.25453339230429 33.0 14.0 35.0 2.0 35.0 86-87 24.937101156251973 33.0 6.0 35.0 2.0 35.0 88-89 24.38534466418146 32.5 2.0 35.0 2.0 35.0 90-91 23.803550894041827 32.0 2.0 35.0 2.0 35.0 92-93 23.471267454350162 32.0 2.0 35.0 2.0 35.0 94-95 23.076372654324885 32.0 2.0 35.0 2.0 35.0 96-97 22.459319517280598 31.0 2.0 35.0 2.0 35.0 98-99 21.82442345359196 31.0 2.0 35.0 2.0 35.0 100-101 20.15547798066595 28.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3478111197571998 1101 2 0.39513878529780655 1101 3 0.29512670916740547 1101 4 0.4283654601199629 1101 5 0.361998341129798 1101 6 0.1275061260373107 1101 7 0.11081923335997601 1101 8 0.4312975172799227 1101 9 0.22743320699504466 1101 10-11 0.4301738427081574 1101 12-13 0.4578506070434969 1101 14-15 0.2734282246488249 1101 16-17 0.5170357240713344 1101 18-19 0.5416159922001071 1101 20-21 0.5616319652112551 1101 22-23 0.584939598855243 1101 24-25 0.3372178888768502 1101 26-27 0.7099332459487897 1101 28-29 0.8782309970418254 1101 30-31 0.9156261965659453 1101 32-33 1.1317748847868643 1101 34-35 0.9961693645630945 1101 36-37 1.0917327091107722 1101 38-39 0.8539775493326545 1101 40-41 0.9326958828187557 1101 42-43 1.0091703497663502 1101 44-45 1.2992016384247336 1101 46-47 1.6837824571912066 1101 48-49 2.170791273675949 1101 50-51 1.974641343273401 1101 52-53 2.273700552186668 1101 54-55 2.494831230344989 1101 56-57 2.6640641186978513 1101 58-59 3.0268782301756794 1101 60-61 2.875624702007965 1101 62-63 3.1087607167017346 1101 64-65 3.131049367511512 1101 66-67 2.7283976122845424 1101 68-69 2.791191421532158 1101 70-71 2.9099841518090166 1101 72-73 2.911584857944842 1101 74-75 2.9031334529531136 1101 76-77 3.025256727499201 1101 78-79 2.8213562125751075 1101 80-81 2.6917183074152966 1101 82-83 3.3937426226582055 1101 84-85 3.333363976861392 1101 86-87 3.63308820959406 1101 88-89 3.1433177678469626 1101 90-91 3.0756528237655836 1101 92-93 2.9073304743403057 1101 94-95 2.7444333129940226 1101 96-97 2.554290563188381 1101 98-99 2.7959621878523926 1101 100-101 2.2655695208840534 1102 1 0.3530309674604659 1102 2 0.3642372868944683 1102 3 0.4247140341489839 1102 4 0.18912448738599608 1102 5 0.08080207485615176 1102 6 0.08848622920606886 1102 7 0.12638052051300974 1102 8 0.20535794469333268 1102 9 0.08524455555804877 1102 10-11 0.34688963735320755 1102 12-13 0.4685359423419442 1102 14-15 0.3725193570487235 1102 16-17 0.28375765971367883 1102 18-19 0.412225179132065 1102 20-21 0.4138918522169135 1102 22-23 0.5013176386489064 1102 24-25 0.6891400210311147 1102 26-27 0.6714721798716567 1102 28-29 0.7376205819104058 1102 30-31 0.8242727207614777 1102 32-33 1.1665697754771074 1102 34-35 1.2443209243764102 1102 36-37 1.2702699235543946 1102 38-39 1.632926210595251 1102 40-41 1.4802701888860597 1102 42-43 1.7963233834190149 1102 44-45 2.2054286096848656 1102 46-47 2.4148067755405123 1102 48-49 2.4990828482276477 1102 50-51 3.0391033919101 1102 52-53 2.6750147256084347 1102 54-55 2.7637762070091973 1102 56-57 2.8688540965902547 1102 58-59 3.1986419245996522 1102 60-61 2.676201955753971 1102 62-63 2.8604433499613755 1102 64-65 2.6186506939669236 1102 66-67 2.7175096240752588 1102 68-69 2.8142938533670865 1102 70-71 2.8195456846019447 1102 72-73 2.7078449905903845 1102 74-75 2.9940386359548334 1102 76-77 3.102240269626442 1102 78-79 3.1297755198706163 1102 80-81 3.220759083637354 1102 82-83 2.998839650415512 1102 84-85 3.383284143896521 1102 86-87 3.2704377551602626 1102 88-89 3.262256603513876 1102 90-91 3.4773968669982054 1102 92-93 3.5397303760843535 1102 94-95 3.59014075588275 1102 96-97 3.521350253682119 1102 98-99 3.5764153934529865 1102 100-101 3.475622333460816 1103 1 0.14784152182453525 1103 2 0.34720992613538826 1103 3 0.38508642642818103 1103 4 0.12834265856945848 1103 5 0.20010733254120794 1103 6 0.14322399485239146 1103 7 0.22180198018676123 1103 8 0.18469114804681652 1103 9 0.21290101880624945 1103 10-11 0.2451316598397213 1103 12-13 0.23808166275529175 1103 14-15 0.20702250906015962 1103 16-17 0.5562847930080252 1103 18-19 0.5747162430171571 1103 20-21 0.7438619568506937 1103 22-23 0.6782283424898097 1103 24-25 0.5175097487233202 1103 26-27 0.27158103799865074 1103 28-29 0.19892720438392075 1103 30-31 0.28856075611599863 1103 32-33 0.13955021350921726 1103 34-35 0.11813485821666347 1103 36-37 0.5485161703861365 1103 38-39 0.753969948815822 1103 40-41 0.9022254108266594 1103 42-43 0.6237101384719779 1103 44-45 0.7596789508819768 1103 46-47 0.6552045138910714 1103 48-49 0.542471747948202 1103 50-51 0.27650346989800667 1103 52-53 0.8505037748058086 1103 54-55 1.1710112105836465 1103 56-57 1.35608357602095 1103 58-59 1.3231463764096283 1103 60-61 0.7850113402993699 1103 62-63 1.2811556395564878 1103 64-65 1.3103303586189092 1103 66-67 1.342070942070972 1103 68-69 1.596853806574341 1103 70-71 1.501866799829081 1103 72-73 1.7110376207327107 1103 74-75 1.5623186775483866 1103 76-77 1.647115813917079 1103 78-79 1.948429253541896 1103 80-81 1.9515951790425667 1103 82-83 2.0568269123899086 1103 84-85 1.9346180621391937 1103 86-87 1.688176755615192 1103 88-89 1.6449822810338581 1103 90-91 1.545668784850939 1103 92-93 1.5056431596029043 1103 94-95 1.352740677894836 1103 96-97 1.2932356114516637 1103 98-99 1.2504502930435457 1103 100-101 0.8496312370807537 1104 1 0.6324839828882354 1104 2 0.5234238226588452 1104 3 0.6087953805725448 1104 4 0.600882318148031 1104 5 0.4467441038416595 1104 6 0.4936989454285978 1104 7 0.5005039426728999 1104 8 0.6525295201959054 1104 9 0.7796110819722628 1104 10-11 0.8800827182321243 1104 12-13 0.7176009259791627 1104 14-15 1.0864043500360978 1104 16-17 1.0818525638745058 1104 18-19 0.8447871239660998 1104 20-21 0.7794832500663702 1104 22-23 0.6745878583777554 1104 24-25 0.9609375899885677 1104 26-27 1.0429658685112102 1104 28-29 1.1784223584414946 1104 30-31 1.3947495791065023 1104 32-33 1.3664295230226955 1104 34-35 1.8448936218986134 1104 36-37 2.029795402750281 1104 38-39 1.839798580861725 1104 40-41 1.2983510678014412 1104 42-43 1.0571929486364127 1104 44-45 1.4260924713024465 1104 46-47 2.002581436597076 1104 48-49 2.2055461488354133 1104 50-51 1.7753703390816717 1104 52-53 1.9795963057860888 1104 54-55 2.0772988811559934 1104 56-57 1.7637816328221447 1104 58-59 1.4703810550344336 1104 60-61 1.5339625915651034 1104 62-63 2.048381638881434 1104 64-65 1.515740455537756 1104 66-67 1.6379747947157455 1104 68-69 1.7808670183974726 1104 70-71 1.7824372227038587 1104 72-73 1.7320969775001558 1104 74-75 1.738954484482182 1104 76-77 1.2905291896825446 1104 78-79 1.320927927537749 1104 80-81 1.745244824637819 1104 82-83 1.896922866871801 1104 84-85 2.2970873229521516 1104 86-87 1.7044022245384731 1104 88-89 1.6549634758841414 1104 90-91 1.8842458618556144 1104 92-93 2.2719650651914094 1104 94-95 2.5151641312918187 1104 96-97 2.217002427595162 1104 98-99 2.1197183387545273 1104 100-101 2.0553909169110263 1105 1 0.10033133688359541 1105 2 0.14570929598795956 1105 3 0.1946060489259125 1105 4 0.17541320577189623 1105 5 0.07419508423381416 1105 6 0.3190204718886491 1105 7 -0.014781741493244738 1105 8 0.35767394355664806 1105 9 0.445645237569984 1105 10-11 0.5433232832753419 1105 12-13 0.5221022964198099 1105 14-15 0.33898710356762507 1105 16-17 0.33174714536301764 1105 18-19 -0.29088650032232977 1105 20-21 -0.006042788305968827 1105 22-23 0.13832389042497795 1105 24-25 0.4287322347281801 1105 26-27 0.26123489655252996 1105 28-29 0.2534276293670743 1105 30-31 -0.4040591917136567 1105 32-33 0.3107158396999026 1105 34-35 0.5011765651834565 1105 36-37 0.6430675933469487 1105 38-39 0.8001612205412556 1105 40-41 1.099373627362894 1105 42-43 0.6290883734730102 1105 44-45 -0.16008716184114036 1105 46-47 0.10236005772294732 1105 48-49 -0.008980522047991712 1105 50-51 0.0968967991280536 1105 52-53 -0.13778720675871625 1105 54-55 -0.06554320191379048 1105 56-57 -0.19210072011903634 1105 58-59 0.21547909425012435 1105 60-61 -0.1533661033537257 1105 62-63 -0.024040878512625596 1105 64-65 -0.14154396360624943 1105 66-67 -0.3279223168170411 1105 68-69 0.024225652173566203 1105 70-71 0.507347616969092 1105 72-73 0.2622993810422187 1105 74-75 0.305526274488507 1105 76-77 0.04938434464775554 1105 78-79 0.2549886486003672 1105 80-81 0.34433822543900305 1105 82-83 -0.23922514071833234 1105 84-85 0.48626084182206597 1105 86-87 0.7435124923014946 1105 88-89 0.208832335255849 1105 90-91 0.4205625390641323 1105 92-93 0.40716430617812804 1105 94-95 0.287249309449102 1105 96-97 0.713734453738951 1105 98-99 0.801301724797117 1105 100-101 0.5199175877944349 1106 1 0.0951590041615411 1106 2 0.31127096430036616 1106 3 -0.03485743910719208 1106 4 -0.30876415821926173 1106 5 -0.07843575227344957 1106 6 0.25888034869308285 1106 7 0.31794031615475404 1106 8 0.4002281354972155 1106 9 0.18651136819489977 1106 10-11 0.3577070703633254 1106 12-13 0.18733481628626691 1106 14-15 0.2734160310139018 1106 16-17 0.0323631231690058 1106 18-19 0.250797799296798 1106 20-21 0.08355612080203656 1106 22-23 -0.0068540848418692235 1106 24-25 0.221110097144134 1106 26-27 -0.1075524265301766 1106 28-29 0.31551913263504616 1106 30-31 0.5794598753856022 1106 32-33 0.40160783198842154 1106 34-35 0.5010590121670475 1106 36-37 0.285635891649477 1106 38-39 0.6322484919006399 1106 40-41 0.33097658029467425 1106 42-43 0.23944714527909383 1106 44-45 0.50954184083907 1106 46-47 0.13000852718267453 1106 48-49 0.4975319150611419 1106 50-51 0.37883595368676026 1106 52-53 0.878200003473097 1106 54-55 1.131217036953938 1106 56-57 1.1168991388173453 1106 58-59 1.0902851317730473 1106 60-61 1.5786365298338403 1106 62-63 1.642061533675271 1106 64-65 1.9149049948740284 1106 66-67 1.7182525080289643 1106 68-69 1.4771809350061211 1106 70-71 1.1544299511017861 1106 72-73 1.4830639458609518 1106 74-75 0.9324835322117409 1106 76-77 1.077738132205944 1106 78-79 1.254071735072369 1106 80-81 1.7365421093906654 1106 82-83 1.8305308192196001 1106 84-85 1.6193778776052064 1106 86-87 1.0044331675260203 1106 88-89 1.4134921368263562 1106 90-91 1.4336579450922535 1106 92-93 1.3503861992519042 1106 94-95 0.9122786977032042 1106 96-97 1.5371463124872484 1106 98-99 1.2492727597338735 1106 100-101 0.9486569492464483 1107 1 0.24623814085218854 1107 2 0.2609112520701551 1107 3 0.08744471916618579 1107 4 -0.07135408627681272 1107 5 -0.028076040043231387 1107 6 -1.1245706230766928E-4 1107 7 -0.09213162629128391 1107 8 0.27073173261951666 1107 9 -0.007733236121644893 1107 10-11 0.462023617125908 1107 12-13 0.36359380909202343 1107 14-15 0.014423225258504146 1107 16-17 0.49998902244957577 1107 18-19 -0.05136047408449684 1107 20-21 0.20226115677326106 1107 22-23 0.12623944033799006 1107 24-25 0.4369374352736273 1107 26-27 0.4607928972108297 1107 28-29 0.006166614649437463 1107 30-31 -0.057230772096410476 1107 32-33 -0.26745691621301404 1107 34-35 0.2420662064116499 1107 36-37 0.06980855351998372 1107 38-39 -0.01523352248784704 1107 40-41 0.49644420619395646 1107 42-43 0.11714498700571596 1107 44-45 1.0599015530692881 1107 46-47 0.2631020523625338 1107 48-49 -0.3118206029244668 1107 50-51 -0.34418562904705396 1107 52-53 -0.37359855767797967 1107 54-55 -0.4766966321108086 1107 56-57 -0.45504330722582154 1107 58-59 -0.6507220624715551 1107 60-61 -0.9537375708855826 1107 62-63 -0.8363557325117803 1107 64-65 -0.7469655087230969 1107 66-67 -0.7781503696688752 1107 68-69 -0.8429629498859654 1107 70-71 -1.5793829985385024 1107 72-73 -1.358662672844087 1107 74-75 -1.2941351728242303 1107 76-77 -0.5985208605998089 1107 78-79 -0.5984462505391441 1107 80-81 -1.0440334301776808 1107 82-83 -1.3025627059602556 1107 84-85 -1.5820609713156557 1107 86-87 -1.0711064008192999 1107 88-89 -0.9606085825981054 1107 90-91 -0.7461981700156635 1107 92-93 -0.9553691964315512 1107 94-95 -0.9186565540953566 1107 96-97 -0.7614148385918895 1107 98-99 0.08380513383459132 1107 100-101 -0.447026503990962 1108 1 -0.10592602945091656 1108 2 -0.2931635208983572 1108 3 -0.02949124026299188 1108 4 -0.36165954682640944 1108 5 -0.25817392894522584 1108 6 -0.08738689980319947 1108 7 0.23679867209295935 1108 8 -0.2404724188134395 1108 9 0.243298725194542 1108 10-11 -0.12079864965082976 1108 12-13 0.14387450257002854 1108 14-15 0.5233865464048719 1108 16-17 0.27844167016747434 1108 18-19 0.09267184104700732 1108 20-21 0.12427436587811513 1108 22-23 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2316 98-99 0.20008053456664143 2316 100-101 0.0015567346812588312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 460.0 3 140.0 4 97.0 5 89.0 6 125.0 7 150.0 8 240.0 9 437.0 10 675.0 11 933.0 12 1156.0 13 1444.0 14 1585.0 15 2112.0 16 2843.0 17 3876.0 18 3414.0 19 1951.0 20 1871.0 21 1856.0 22 1928.0 23 2015.0 24 2176.0 25 2179.0 26 2359.0 27 2617.0 28 2909.0 29 3333.0 30 4016.0 31 4815.0 32 5926.0 33 7737.0 34 10727.0 35 19114.0 36 20517.0 37 23473.0 38 14948.0 39 2027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.704091822675046 17.796331643681505 16.94939893058944 14.550177603054015 2 13.557811433339234 24.328082728628857 38.92126621324366 23.19283962478825 3 23.50989401303191 30.977014036795236 33.85958148735677 11.65351046281608 4 9.80014158218087 17.56946375748995 42.23763052107299 30.39276413925619 5 9.476419597751658 39.84104804597847 35.11801265798775 15.564519698282131 6 21.56214439246428 38.312049563788094 20.04235680869895 20.08344923504868 7 18.18394481781914 42.21929985395184 21.692894219401012 17.90386110882801 8 21.46158563958598 25.881876869186947 23.419766434618765 29.23677105660831 9 23.62558163058871 21.603150920493626 26.49580214444669 28.27546530447097 10-11 21.5801070391012 25.359352328084643 26.89444351363911 26.166097119175046 12-13 31.874180036355014 25.986880581680232 26.04251956057852 16.09641982138623 14-15 26.090997316170313 24.793971024755088 35.8340261554851 13.281005503589505 16-17 15.401343342552352 29.89111023310944 35.09537732121691 19.612169103121296 18-19 13.766597448078159 30.675845241143023 40.270363381786346 15.287193928992474 20-21 17.021417824586248 25.99028991187589 36.851238415536145 20.13705384800172 22-23 15.023432636305891 25.836575137704266 37.78642961202633 21.35356261396351 24-25 18.333917890286557 25.34702144534077 39.03892517355022 17.280135490822456 26-27 19.051094659879745 22.113425026304064 42.75950974909239 16.0759705647238 28-29 14.783768156239239 26.849274382364 42.84919855034456 15.517758911052201 30-31 14.85312749803317 28.231052862726262 40.5878059646318 16.328013674608766 32-33 14.346150200625573 28.16972607500553 39.559887523301 17.9242362010679 34-35 16.919008410689347 25.184359024587522 38.58585411782548 19.31077844689765 36-37 17.619014520592955 24.521983090902197 42.209872486698934 15.64912990180591 38-39 19.5661377667406 24.245669908311374 40.68832818335197 15.499864141596051 40-41 15.51495352496256 26.73341463568752 42.60917368584012 15.142458153509796 42-43 14.124181333939928 29.574972908590585 38.21452605040424 18.08631970706525 44-45 15.149495153728532 28.57720546421847 36.90843263872215 19.364866743330847 46-47 16.418240523685256 27.928511401906952 35.04893752803877 20.604310546369017 48-49 16.81341281951645 26.638928642511207 36.25877870553781 20.288879832434535 50-51 17.03373257213627 27.8122324410225 38.71110423070969 16.442930756131542 52-53 15.61602547698257 29.047993656029142 36.842737132765286 18.493243734223004 54-55 13.514136860097373 26.206822468587387 39.500431261907096 20.778609409408144 56-57 17.356024747375805 28.792838680729783 33.756216861835576 20.094919710058832 58-59 17.414615651954122 26.521436921424286 35.41847019051531 20.645477236106284 60-61 16.901021259384716 24.451768244900023 38.949733309739884 19.69747718597538 62-63 19.730231852736544 24.953711602747603 36.47399318786454 18.842063356651312 64-65 17.929720575783232 27.01195546400091 38.35637645809901 16.70194750211685 66-67 16.770885008041507 25.569777839568005 37.18461971101134 20.47471744137916 68-69 15.184589472885904 28.385983650330694 37.501224487216355 18.928202389567055 70-71 17.39420309088439 27.42551638497341 33.56526025423488 21.615020269907323 72-73 15.759421874358157 25.14227211972029 35.95558518790031 23.14272081802124 74-75 15.868170758681707 28.058899737731856 36.11147976111479 19.96144974247164 76-77 17.65936402905887 24.48358565510442 36.02845216314174 21.828598152694962 78-79 16.019328495942155 24.473807296538823 39.0024144818345 20.504449725684523 80-81 17.5547648241079 27.077144771270746 36.192817686541694 19.17527271807966 82-83 15.624881495620077 25.6383435931792 36.718977133394844 22.017797777805868 84-85 16.491242483513496 24.33461517800999 35.28032584344221 23.8938164950343 86-87 18.036371680017695 25.01461503215307 36.171967578328676 20.77704570950056 88-89 19.129157121415595 22.950943992416462 34.94936408879059 22.970534797377358 90-91 16.750770203017616 22.949364088790585 37.91199936803855 22.387866340153252 92-93 17.1300904727144 26.902913265644703 36.58314609937146 19.383850162269432 94-95 16.743346836626998 27.532183552103547 34.75634033355874 20.968129277710716 96-97 14.384630942900124 26.54095086401962 34.54119104504026 24.533227148039995 98-99 17.586689718604116 26.650273241315226 32.35120279910363 23.41183424097703 100-101 19.95871635034835 24.369681490004677 34.14341168586493 21.52819047378204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2085.0 1 1448.5 2 911.0 3 920.0 4 675.0 5 641.0 6 1135.5 7 2437.0 8 3980.5 9 4086.5 10 2665.0 11 1484.0 12 1227.5 13 1212.0 14 1111.0 15 979.0 16 907.5 17 903.0 18 886.0 19 857.0 20 845.0 21 934.5 22 973.5 23 960.5 24 1057.5 25 1190.5 26 1287.0 27 1362.5 28 1451.0 29 1537.0 30 1683.0 31 1807.5 32 1899.5 33 1969.5 34 2085.0 35 2240.5 36 2338.5 37 2486.5 38 2439.5 39 2151.0 40 1962.5 41 1870.5 42 1782.5 43 1733.0 44 1811.5 45 2221.0 46 4015.0 47 9257.0 48 25195.5 49 24425.5 50 8010.0 51 3246.5 52 1574.0 53 1122.5 54 1002.0 55 936.0 56 876.0 57 789.0 58 735.5 59 686.5 60 674.5 61 656.0 62 534.0 63 321.0 64 168.5 65 125.0 66 97.0 67 63.0 68 37.0 69 28.0 70 22.0 71 20.5 72 14.5 73 11.0 74 10.0 75 6.5 76 7.0 77 4.5 78 2.5 79 2.5 80 1.5 81 0.5 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032855247362102734 2 0.04170089088266886 3 0.0271687622417388 4 0.03664623744234536 5 0.06886965312440765 6 0.05686485120363935 7 0.06507866304416504 8 0.0713969798445694 9 0.0593921779238011 10-11 0.06539457888418525 12-13 0.067921905604347 14-15 0.06349908384406394 16-17 0.05212611360333608 18-19 0.04770329184305301 20-21 0.05307386112339673 22-23 0.03190749984204208 24-25 0.019270866241233336 26-27 0.015479876160990714 28-29 0.016111707841031147 30-31 0.012952549440828964 32-33 0.009477475200606558 34-35 0.012004801920768308 36-37 0.007581980160485247 38-39 0.010741138560687433 40-41 0.008213811840525683 42-43 0.006002400960384154 44-45 0.0025273267201617487 46-47 0.004422821760283061 48-49 0.0022114108801415304 50-51 0.006634232640424591 52-53 0.006002400960384154 54-55 0.008529727680545903 56-57 0.019586782081253556 58-59 0.009477475200606558 60-61 0.021482277121374866 62-63 0.014532128640930056 64-65 0.010109306880646995 66-67 0.01800720288115246 68-69 0.025589183041637705 70-71 0.019270866241233336 72-73 0.021798192961395086 74-75 0.022114108801415303 76-77 0.024957351361597272 78-79 0.03664623744234536 80-81 0.030327920641940988 82-83 0.030327920641940988 84-85 0.021798192961395086 86-87 0.02685284640171858 88-89 0.02053452960131421 90-91 0.02053452960131421 92-93 0.028748341441839895 94-95 0.02337777216149618 96-97 0.03538257408226449 98-99 0.04833512352309345 100-101 0.060655841283881975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 158270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.61154988311114 #Duplication Level Percentage of deduplicated Percentage of total 1 85.13825967629754 28.616288620711444 2 8.320018046130421 5.59297403171795 3 2.4869823486286817 2.5077399380804954 4 1.1316427618098766 1.5214506855373728 5 0.6278549542267422 1.0551589056675301 6 0.43423501325262703 0.8757187085360459 7 0.2594131248002707 0.6103494029190624 8 0.19361994097411508 0.5206293043533203 9 0.16166325168712523 0.4890377203512984 >10 0.9774987311314548 6.248183483919884 >50 0.08647104160009023 1.9795286535666898 >100 0.09962967836532136 6.816831995956277 >500 0.04323552080004511 10.046123712642952 >1k 0.03759610504351749 29.442724458204335 >5k 0.0018798052521758748 3.6772603778353443 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5820 3.6772603778353443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3934 2.4856258292790803 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3706 2.3415682062298604 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3658 2.3112402855879193 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3151 1.9909016238074178 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3064 1.9359322676438997 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3050 1.9270866241233335 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2948 1.8626397927592089 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2840 1.7944019713148418 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2747 1.7356416250710809 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2362 1.4923864282555126 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2143 1.354015290326657 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2043 1.2908321223226134 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1970 1.2447084096796612 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1790 1.1309787072723827 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1607 1.0153535098249826 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1285 0.8119037088519618 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1199 0.7575661843684842 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1042 0.6583686106021356 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1033 0.6526821254817716 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1027 0.648891135401529 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 991 0.6261451949200734 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 969 0.6122448979591837 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 886 0.5598028685158274 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 863 0.5452707398748974 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 821 0.518733809313199 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 807 0.5098881657926329 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 779 0.4921968787515006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.49030138371137927 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 745 0.4707146016301257 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 709 0.44796866114867 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 693 0.43785935426802297 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 661 0.41764074050672895 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 638 0.40310861186579894 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 593 0.3746761862639793 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 581 0.367094206103494 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 580 0.3664623744234536 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 579 0.36583054274341315 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 578 0.3651987110633727 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 563 0.35572123586276616 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 544 0.34371643394199786 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 522 0.32981613698110823 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 513 0.3241296518607443 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 509 0.32160232514058257 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 497 0.31402034498009734 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 452 0.2855879193782776 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 445 0.2811650976179946 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 442 0.27926960257787325 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 418 0.26410564225690275 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 412 0.26031465217666017 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 334 0.21103178113350604 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 325 0.20534529601314208 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 320 0.2021861376129399 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.18575851393188852 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 294 0.18575851393188852 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 274 0.1731218803310798 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 248 0.15669425665002845 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 247 0.15606242496998798 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 235 0.14848044480950273 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 233 0.14721678144942188 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 212 0.1339483161685727 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 211 0.13331648448853226 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 208 0.13142098944841094 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 208 0.13142098944841094 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 201 0.12699816768812788 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 186 0.11752069248752134 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 185 0.11688886080748088 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.1156251974474 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 0.11499336576735958 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.10425222720667215 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.10298856384659126 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 162 0.10235673216655082 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 161 0.1017249004865104 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 161 0.1017249004865104 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.1591584002021864E-4 0.0 14-15 0.0 0.0 0.0 6.318316800404373E-4 0.0 16-17 0.0 0.0 0.0 6.318316800404373E-4 0.0 18-19 0.0 0.0 0.0 0.001579579200101093 0.0 20-21 0.0 0.0 0.0 0.003475074240222405 0.0 22-23 0.0 0.0 0.0 0.007266064320465029 0.0 24-25 0.0 0.0 0.0 0.0135843811208694 0.0 26-27 0.0 0.0 0.0 0.028116509761799458 0.0 28-29 0.0 0.0 0.0 0.05338977696341694 0.0 30-31 0.0 0.0 0.0 0.09098376192582297 0.0 32-33 0.0 0.0 0.0 0.11341378656725848 0.0 34-35 0.0 0.0 0.0 0.13363240032855248 0.0 36-37 0.0 0.0 0.0 0.15321918240980603 0.0 38-39 0.0 0.0 0.0 0.19555190497251534 0.0 40-41 0.0 0.0 0.0 0.26726480065710495 0.0 42-43 0.0 0.0 0.0 0.3895242307449296 0.0 44-45 0.0 0.0 0.0 0.4972515321918241 0.0 46-47 0.0 0.0 0.0 0.7762052189296771 0.0 48-49 0.0 0.0 0.0 1.021355910785367 0.0 50-51 0.0 0.0 0.0 1.166045365514627 0.0 52-53 0.0 0.0 0.0 1.3262146964048775 0.0 54-55 0.0 0.0 0.0 1.4765906362545018 0.0 56-57 0.0 0.0 0.0 1.845264421558097 0.0 58-59 0.0 0.0 0.0 2.3314588993492134 0.0 60-61 0.0 6.318316800404373E-4 0.0 2.9045302331458895 0.0 62-63 0.0 6.318316800404373E-4 0.0 3.4324256018196753 0.0 64-65 0.0 6.318316800404373E-4 0.0 3.8949263916092756 0.0 66-67 0.0 6.318316800404373E-4 0.0 4.549504012131168 0.0 68-69 0.0 6.318316800404373E-4 0.0 5.229038984014659 0.0 70-71 0.0 6.318316800404373E-4 0.0 5.659948189802236 0.0 72-73 0.0 6.318316800404373E-4 0.0 6.162254375434384 0.0 74-75 0.0 6.318316800404373E-4 0.0 6.6955203133885135 0.0 76-77 0.0 6.318316800404373E-4 0.0 7.1779238010993875 0.0 78-79 0.0 6.318316800404373E-4 0.0 7.682441397611676 0.0 80-81 0.0 6.318316800404373E-4 0.0 8.376824413976117 0.0 82-83 0.0 6.318316800404373E-4 0.0 9.14860681114551 0.0 84-85 0.0 6.318316800404373E-4 0.0 10.562330195235988 0.0 86-87 0.0 6.318316800404373E-4 0.0 11.83515511467745 0.0 88-89 0.0 6.318316800404373E-4 0.0 12.935174069627852 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 5.2448067E-5 76.048065 1 GATTGGC 25 5.253038E-5 76.024025 9 GGATATG 30 1.2937181E-4 63.37339 1 ACTACCT 30 1.2937181E-4 63.37339 5 GAGATTG 30 1.2957449E-4 63.353355 7 AGATTGG 35 2.7762412E-4 54.30288 8 GATATGA 40 5.3572794E-4 47.530045 2 TACCTCC 45 9.584953E-4 42.23557 7 ATGAGAT 50 0.0016066049 38.024033 5 GTCACTA 50 0.0016066049 38.024033 2 ATATGAG 50 0.0016091041 38.012012 3 TGAGATT 50 0.0016091041 38.012012 6 CACTACC 55 0.0025689462 34.556377 4 TCACTAC 55 0.0025689462 34.556377 3 TTATTGT 70 2.0415682E-4 33.9393 4 GGGATGG 60 0.003934653 31.676678 7 ATGGGGT 65 0.005811056 29.249256 5 TATGAGA 65 0.0058200476 29.24001 4 CTACCTC 65 0.0058200476 29.24001 6 ATGGGAC 100 4.905314E-5 28.518024 5 >>END_MODULE