##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158270 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.992639160927528 31.0 31.0 34.0 31.0 34.0 2 31.97016490806849 31.0 31.0 34.0 30.0 34.0 3 32.08168951791243 33.0 31.0 34.0 30.0 34.0 4 35.76813672837556 37.0 35.0 37.0 35.0 37.0 5 35.70821381184052 37.0 35.0 37.0 35.0 37.0 6 35.88458330700701 37.0 35.0 37.0 35.0 37.0 7 35.854735578441904 37.0 35.0 37.0 35.0 37.0 8 35.8984267391167 37.0 35.0 37.0 35.0 37.0 9 37.45137423390409 39.0 37.0 39.0 34.0 39.0 10-11 37.35608769823719 39.0 37.0 39.0 34.0 39.0 12-13 37.290392999304984 39.0 37.0 39.0 34.0 39.0 14-15 38.00307702028179 40.0 37.0 41.0 33.0 41.0 16-17 38.08538573324067 39.0 37.0 41.0 33.5 41.0 18-19 38.27203828899981 40.0 37.0 41.0 34.0 41.0 20-21 38.39957667277437 40.0 37.0 41.0 34.0 41.0 22-23 38.35852972768055 40.0 37.5 41.0 34.0 41.0 24-25 38.15430277374108 40.0 37.0 41.0 34.0 41.0 26-27 37.64670499778859 39.5 36.0 41.0 33.0 41.0 28-29 37.46031465217666 39.0 36.0 41.0 33.0 41.0 30-31 37.28295002211411 39.0 35.5 41.0 33.0 41.0 32-33 36.97458772982877 39.0 35.0 41.0 33.0 41.0 34-35 36.69237379162191 38.5 35.0 41.0 33.0 41.0 36-37 36.29446515448284 38.0 35.0 40.0 31.5 41.0 38-39 35.92534276868642 38.0 35.0 40.0 30.5 41.0 40-41 35.59697352625261 38.0 35.0 40.0 30.0 41.0 42-43 35.31029885638466 38.0 35.0 40.0 29.5 41.0 44-45 34.86234599102799 38.0 35.0 40.0 26.0 41.0 46-47 34.20801478486131 38.0 34.0 40.0 23.0 41.0 48-49 33.55275478612498 37.0 33.0 40.0 19.0 41.0 50-51 33.13371769760536 37.0 33.0 40.0 12.0 41.0 52-53 32.74326467429077 37.0 33.0 40.0 6.5 41.0 54-55 32.536946357490365 37.0 33.0 40.0 2.0 41.0 56-57 32.22023125039489 36.0 32.5 40.0 2.0 41.0 58-59 31.888996651292096 36.0 32.0 40.0 2.0 41.0 60-61 31.259126808618184 35.0 31.0 39.0 2.0 40.5 62-63 30.8344000758198 35.0 31.0 39.0 2.0 40.0 64-65 30.205683325961964 35.0 30.0 38.0 2.0 40.0 66-67 29.676998167688126 35.0 29.0 37.0 2.0 39.0 68-69 29.139442092626524 35.0 29.0 37.0 2.0 39.0 70-71 28.572793327857458 34.0 28.0 36.0 2.0 38.5 72-73 28.116418146205852 34.0 27.0 35.0 2.0 37.0 74-75 27.639094585202503 34.0 26.0 35.0 2.0 37.0 76-77 26.15438807101788 31.5 25.0 34.0 2.0 35.5 78-79 26.792297971820307 33.0 25.5 35.0 2.0 36.0 80-81 26.442247425285906 33.0 25.0 35.0 2.0 36.0 82-83 26.260248309850255 33.0 24.5 35.0 2.0 35.0 84-85 25.952239843305744 33.0 24.0 35.0 2.0 35.0 86-87 25.61082959499589 33.0 20.0 35.0 2.0 35.0 88-89 25.06079800341189 32.5 18.0 35.0 2.0 35.0 90-91 24.43071017880837 32.0 9.0 34.0 2.0 35.0 92-93 24.119798445694066 32.0 2.0 34.0 2.0 35.0 94-95 23.58810576862324 31.0 2.0 34.0 2.0 35.0 96-97 22.814323624186517 31.0 2.0 34.0 2.0 35.0 98-99 22.12392746572313 31.0 2.0 34.0 2.0 35.0 100-101 19.494575725026852 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43693008653341536 1101 2 0.29466094879122906 1101 3 0.5229564645165112 1101 4 0.29635853484584374 1101 5 0.3472065610301911 1101 6 0.19834227915925595 1101 7 0.3048320513894325 1101 8 0.2185031003139315 1101 9 0.45968427932439226 1101 10-11 0.38849707398905053 1101 12-13 0.42324118283710277 1101 14-15 0.5064490394123666 1101 16-17 0.4074668760122222 1101 18-19 0.5980749170424318 1101 20-21 0.340710271491389 1101 22-23 0.42636288902335906 1101 24-25 0.47984288246625795 1101 26-27 0.6324581663353399 1101 28-29 0.8803767561235318 1101 30-31 0.8714940137808327 1101 32-33 0.7219451450561323 1101 34-35 1.069985769305319 1101 36-37 1.092067807062108 1101 38-39 1.39997321183845 1101 40-41 1.785407789272206 1101 42-43 1.5074301901290426 1101 44-45 1.6113136561569092 1101 46-47 1.4353851209441757 1101 48-49 1.5356760891668912 1101 50-51 1.3710263500301778 1101 52-53 1.813080058254215 1101 54-55 1.9866501716300746 1101 56-57 2.0788898161457148 1101 58-59 2.1781927513237314 1101 60-61 2.4497305483611065 1101 62-63 2.5730150643795007 1101 64-65 2.643575679359632 1101 66-67 2.824399625114939 1101 68-69 3.0205279364390236 1101 70-71 2.643464558509745 1101 72-73 2.5134229732810134 1101 74-75 2.486018414121215 1101 76-77 2.2627366876244004 1101 78-79 2.635071883275053 1101 80-81 2.8136696267680534 1101 82-83 2.7039599348438053 1101 84-85 2.6315163269639648 1101 86-87 2.5248045440076012 1101 88-89 2.3710432057607598 1101 90-91 2.312496004756241 1101 92-93 2.641703455864839 1101 94-95 2.9717564747508263 1101 96-97 2.5186139143992214 1101 98-99 2.382366546955243 1101 100-101 2.375563110750246 1102 1 0.255611000313813 1102 2 0.15040892226212677 1102 3 0.07864302801442591 1102 4 0.058628247447167325 1102 5 0.003237020376786859 1102 6 0.29896866088364504 1102 7 0.32702561562426524 1102 8 0.3769776765851205 1102 9 0.3769527637262584 1102 10-11 0.4386137529377123 1102 12-13 0.2539830153106948 1102 14-15 0.2649850256565358 1102 16-17 0.08635412730183134 1102 18-19 0.4922655335985624 1102 20-21 0.46826492636733974 1102 22-23 0.586520098554189 1102 24-25 0.6668976601621566 1102 26-27 0.814121148403629 1102 28-29 0.8308496129154719 1102 30-31 1.0836224834443016 1102 32-33 0.9177631258962151 1102 34-35 0.7308000655470295 1102 36-37 1.1153667508105727 1102 38-39 1.3045912418065129 1102 40-41 1.6467195062950495 1102 42-43 1.0215667659089434 1102 44-45 0.6211638158228396 1102 46-47 0.9183377124450303 1102 48-49 1.0770172829767617 1102 50-51 0.909929567912755 1102 52-53 1.2033711415261479 1102 54-55 1.3705853226986164 1102 56-57 1.4587104990246083 1102 58-59 1.469742739041731 1102 60-61 1.2054416758482098 1102 62-63 1.1768777516799176 1102 64-65 1.2976471605681787 1102 66-67 1.300517286623169 1102 68-69 1.489558887065403 1102 70-71 1.3485800092589493 1102 72-73 1.2347322339049391 1102 74-75 1.3804792345584538 1102 76-77 1.6502090531365567 1102 78-79 1.4976486457412754 1102 80-81 1.4558889831915387 1102 82-83 1.8935570854210617 1102 84-85 1.855576754377374 1102 86-87 2.0264994592618386 1102 88-89 1.6698641342795533 1102 90-91 1.4541995174066962 1102 92-93 1.5967022276653786 1102 94-95 1.6601806948441649 1102 96-97 1.6760875081108395 1102 98-99 2.0655893423372085 1102 100-101 2.1139689078516923 1103 1 0.10670283108004952 1103 2 0.047416136143972665 1103 3 0.0489426315758692 1103 4 -0.1006620625547825 1103 5 -0.06873932328993959 1103 6 0.036588079873702384 1103 7 0.21538916927345042 1103 8 0.07765032232502733 1103 9 -0.08174613794398056 1103 10-11 -0.037413087901960296 1103 12-13 0.36246745022328497 1103 14-15 0.34079577499039004 1103 16-17 0.09459160049348725 1103 18-19 0.23917851208688745 1103 20-21 0.41582617937311994 1103 22-23 0.5341382177457135 1103 24-25 0.5415970817518101 1103 26-27 0.5216502313957605 1103 28-29 0.702372651844442 1103 30-31 0.6655504096157472 1103 32-33 1.0038179124043083 1103 34-35 1.180960915615266 1103 36-37 1.0947811915722525 1103 38-39 1.2625102643580206 1103 40-41 1.1841537039944043 1103 42-43 1.4151295136830413 1103 44-45 1.3354680986589926 1103 46-47 1.232466522479477 1103 48-49 1.2464460215222957 1103 50-51 1.6395146072316606 1103 52-53 1.9843957077183845 1103 54-55 1.6914080951688817 1103 56-57 1.8313789854092128 1103 58-59 2.1417710746497143 1103 60-61 1.881401902013149 1103 62-63 1.829790925138031 1103 64-65 2.1190792135999637 1103 66-67 1.9534792025262036 1103 68-69 2.500014141500966 1103 70-71 2.1426513032083854 1103 72-73 2.252945660823766 1103 74-75 1.8829326042861467 1103 76-77 1.4090314356672664 1103 78-79 1.8171878671619588 1103 80-81 2.732169284744799 1103 82-83 2.5855210810017475 1103 84-85 2.7441027828429654 1103 86-87 2.600490490651975 1103 88-89 2.2017416932579117 1103 90-91 2.481113470743928 1103 92-93 2.478907985772871 1103 94-95 3.1114463736639486 1103 96-97 2.7438476302918993 1103 98-99 2.6038684090818656 1103 100-101 1.2495541421403793 1104 1 0.35039828409128404 1104 2 0.3377628259554406 1104 3 0.1653657956628578 1104 4 0.2867722399670356 1104 5 0.3922038272959085 1104 6 0.2651911947779908 1104 7 0.2736128059000933 1104 8 0.24525724647759262 1104 9 0.3561486496542656 1104 10-11 0.37766237378857426 1104 12-13 0.24498348281888127 1104 14-15 0.5054011079379777 1104 16-17 0.5366813830287995 1104 18-19 0.4005170530001507 1104 20-21 0.5074771750816964 1104 22-23 0.5889745165065037 1104 24-25 0.3264348270625774 1104 26-27 0.4113628501334077 1104 28-29 0.19603194110622013 1104 30-31 0.9985944329534249 1104 32-33 0.6244601805946743 1104 34-35 0.8653839832655876 1104 36-37 0.7604111640878131 1104 38-39 0.7446333175428421 1104 40-41 1.1362005722137027 1104 42-43 0.8309403049458837 1104 44-45 0.2710001879593449 1104 46-47 0.8385106904721553 1104 48-49 0.9525432296808347 1104 50-51 1.3801843598716204 1104 52-53 1.471545522077804 1104 54-55 2.166985509701888 1104 56-57 1.9993837108058123 1104 58-59 1.725586591309149 1104 60-61 1.8329545323231962 1104 62-63 1.407651477720126 1104 64-65 1.71519040507496 1104 66-67 1.6887243927475275 1104 68-69 1.6756855817825596 1104 70-71 1.5924439643781625 1104 72-73 1.8284676242742677 1104 74-75 1.651400225675797 1104 76-77 1.6564035365400187 1104 78-79 1.8149698238618974 1104 80-81 1.8629132936169697 1104 82-83 2.0934168329666427 1104 84-85 1.703285964288547 1104 86-87 1.5211231151758149 1104 88-89 1.8759183652285927 1104 90-91 1.6712986291411518 1104 92-93 1.4122702501057702 1104 94-95 1.8363728407067192 1104 96-97 2.0374128938050866 1104 98-99 2.1091844802521216 1104 100-101 2.5599717129007793 1105 1 0.2995865615528075 1105 2 0.23772065855084534 1105 3 0.34320682454752927 1105 4 0.0930130158472835 1105 5 0.18041803771125586 1105 6 0.31452705815559767 1105 7 0.24525522630911922 1105 8 0.1073698334478621 1105 9 0.040209370716880244 1105 10-11 0.27176947899625503 1105 12-13 0.17519642821208947 1105 14-15 0.20701401116378548 1105 16-17 0.22495884455104687 1105 18-19 0.15246729546272064 1105 20-21 0.3094485012465711 1105 22-23 0.24125155635470463 1105 24-25 0.07738359366599212 1105 26-27 0.27099810208365227 1105 28-29 0.3450937163539507 1105 30-31 0.1498699969424635 1105 32-33 0.2353604546827981 1105 34-35 0.15396715847115416 1105 36-37 0.3724710418023349 1105 38-39 0.5896153963958852 1105 40-41 0.24362203542263927 1105 42-43 0.404417007454839 1105 44-45 0.5363385813812371 1105 46-47 0.10390179314978099 1105 48-49 -0.4549520264861471 1105 50-51 -0.07369504135123606 1105 52-53 0.055353238712839925 1105 54-55 -0.6230208154088039 1105 56-57 -0.9367853804866151 1105 58-59 0.1406719803034555 1105 60-61 -0.2969737830889905 1105 62-63 -0.9183552690646124 1105 64-65 -1.0816154140268743 1105 66-67 -0.55210419675279 1105 68-69 -0.6630768048925404 1105 70-71 -0.1763205306671658 1105 72-73 -0.1475496643616161 1105 74-75 -0.38628689218209544 1105 76-77 -0.43220072236784546 1105 78-79 -0.2692595668191089 1105 80-81 -0.10588282698180507 1105 82-83 -0.0015125366306563137 1105 84-85 -0.5656366585240136 1105 86-87 -0.10095152113031602 1105 88-89 0.514280161560027 1105 90-91 0.7767698498875149 1105 92-93 0.36282896821667165 1105 94-95 0.4463770574471795 1105 96-97 0.16660327963684196 1105 98-99 0.7849698481627279 1105 100-101 1.4860206270901095 1106 1 -0.03708527744658241 1106 2 0.16784714559650027 1106 3 -0.1451084056212757 1106 4 -0.07649843381651777 1106 5 -0.08064370113295638 1106 6 0.09291611161871316 1106 7 0.05627699712776035 1106 8 0.24208559025418452 1106 9 0.14121092241669686 1106 10-11 0.3630611515405704 1106 12-13 0.16402889165310341 1106 14-15 0.2777574164395631 1106 16-17 -0.03680821239162668 1106 18-19 0.23543621444518692 1106 20-21 0.306251059200207 1106 22-23 0.36813828226808454 1106 24-25 0.1183625751866586 1106 26-27 0.07402626778638677 1106 28-29 0.3491610507217473 1106 30-31 0.08368764870340328 1106 32-33 0.8132510833563344 1106 34-35 0.8712051327975772 1106 36-37 1.0025787203653636 1106 38-39 0.5960102804886063 1106 40-41 0.6109046798952988 1106 42-43 0.9563754407082428 1106 44-45 1.151869685909375 1106 46-47 1.6137292253216913 1106 48-49 2.0027288876061036 1106 50-51 1.6586132296789984 1106 52-53 1.4781209069312098 1106 54-55 1.2235724981756206 1106 56-57 1.4181777078662314 1106 58-59 1.3489524147794008 1106 60-61 1.6414754575185277 1106 62-63 1.8120956643063444 1106 64-65 2.3108235759576097 1106 66-67 1.5482625686584122 1106 68-69 1.4630105605092574 1106 70-71 2.068095701053341 1106 72-73 1.9419316917299803 1106 74-75 1.8812872837712575 1106 76-77 1.6921233477877955 1106 78-79 1.5540817601193417 1106 80-81 1.6137062076728235 1106 82-83 1.2425040148340507 1106 84-85 0.8615980243179457 1106 86-87 1.0516793153769477 1106 88-89 0.7447781161375424 1106 90-91 0.8644408895263886 1106 92-93 0.4816985784108674 1106 94-95 0.7552828739704296 1106 96-97 0.5389407038151468 1106 98-99 1.0437980796951507 1106 100-101 0.4993041179444866 1107 1 -0.05147376665521719 1107 2 -0.04078594792867918 1107 3 -0.36093574375077253 1107 4 -0.27074303813306244 1107 5 -0.15978039178044412 1107 6 0.18644129147482857 1107 7 -0.16674458560605387 1107 8 -0.009712970896892159 1107 9 -0.160947350964598 1107 10-11 0.08608273427437751 1107 12-13 0.03812961107756507 1107 14-15 0.11948403514460182 1107 16-17 -0.05119670160026857 1107 18-19 0.19586786912145016 1107 20-21 0.14438055560308527 1107 22-23 0.047994397375994424 1107 24-25 0.05001725144565228 1107 26-27 0.08841475699501444 1107 28-29 0.17649918021815836 1107 30-31 0.3067092314372175 1107 32-33 -0.48531006772279994 1107 34-35 -0.4201617736772434 1107 36-37 -0.2168457400662902 1107 38-39 -0.41118396411570046 1107 40-41 0.14687590291688224 1107 42-43 0.19738263495284514 1107 44-45 -0.15388570977407312 1107 46-47 -0.6704434365488154 1107 48-49 -0.8030265080773447 1107 50-51 -1.4385090724792775 1107 52-53 -1.7017352081767037 1107 54-55 -1.2512476457092703 1107 56-57 -1.2832611410546342 1107 58-59 -1.431623124788942 1107 60-61 -1.6786684273735588 1107 62-63 -2.1843072133915022 1107 64-65 -1.4445721074956275 1107 66-67 -1.4265575752264787 1107 68-69 -1.1700829646705984 1107 70-71 -1.327587752184069 1107 72-73 -0.986125862226853 1107 74-75 -1.1474896946460085 1107 76-77 -0.5632723356654417 1107 78-79 -0.316421837002963 1107 80-81 -0.85391969160775 1107 82-83 -1.3114528196983208 1107 84-85 -0.8614235584158685 1107 86-87 -0.25767320260866455 1107 88-89 -0.2480276392581402 1107 90-91 -0.5923936428477106 1107 92-93 -0.8995963856179081 1107 94-95 -0.39939338502237476 1107 96-97 -0.5473902314366548 1107 98-99 -0.5749069562760738 1107 100-101 0.30146239132578145 1108 1 0.1609923260443935 1108 2 0.2300596706584166 1108 3 -0.03919968997084666 1108 4 -0.068242743286973 1108 5 -0.3283144170192642 1108 6 0.10848398518870539 1108 7 -0.11850776522629047 1108 8 -0.0697436348902869 1108 9 0.0924433790743251 1108 10-11 0.13352347021022837 1108 12-13 -0.18816476325903864 1108 14-15 -0.03752150796190534 1108 16-17 -0.4307520736960342 1108 18-19 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-0.7467623443624909 2316 54-55 -0.33832009301458044 2316 56-57 -0.021838700060058613 2316 58-59 0.6404139823674377 2316 60-61 0.6781724157414217 2316 62-63 0.9480447997348378 2316 64-65 0.8945197768915989 2316 66-67 0.39281324390768546 2316 68-69 -0.08004446915122543 2316 70-71 0.8656471335915192 2316 72-73 0.6626498540422361 2316 74-75 0.39630684706798647 2316 76-77 0.6473486412372473 2316 78-79 1.01071119341481 2316 80-81 1.331079677318158 2316 82-83 0.8730574668093141 2316 84-85 0.45788415330948595 2316 86-87 1.0045696347004345 2316 88-89 0.7715041037684891 2316 90-91 0.1312590139741978 2316 92-93 -0.1695067730973996 2316 94-95 -0.32894973993844445 2316 96-97 -1.4531077626748221 2316 98-99 -1.9677821298214475 2316 100-101 -1.9318930296006336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 10.0 6 37.0 7 61.0 8 142.0 9 209.0 10 435.0 11 651.0 12 829.0 13 1092.0 14 1412.0 15 1936.0 16 2609.0 17 3419.0 18 3127.0 19 2063.0 20 1825.0 21 1852.0 22 1839.0 23 1957.0 24 2182.0 25 2181.0 26 2457.0 27 2825.0 28 3330.0 29 3924.0 30 4578.0 31 5554.0 32 6800.0 33 8731.0 34 12483.0 35 19089.0 36 23385.0 37 23567.0 38 11056.0 39 619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.41137844516493 19.431962535312746 15.679805851013404 12.476853168508933 2 13.74194363705295 29.52040945279919 35.61228358397573 21.125363326172124 3 26.943054291780406 32.82592761095946 29.438378323728642 10.792639773531493 4 10.50672900739243 18.830479560245152 40.46755544322993 30.195235989132495 5 10.274604458373352 45.45190314916848 30.190062174594352 14.083430217863825 6 22.98098186643078 41.62001642762368 16.33095343400518 19.068048271940356 7 22.68465280849182 42.382005433752454 18.96190054969356 15.971441208062172 8 24.10564225690276 28.5828015416693 19.518544259809186 27.79301194161875 9 25.459025715549377 25.5531686358754 22.23036583054274 26.757439818032474 10-11 23.635875402792696 28.549946294307198 23.87628735704808 23.937890945852025 12-13 33.99317621785556 28.993176217855567 22.10399949453466 14.909648069754217 14-15 26.31136665192393 28.913249510330445 31.709736526189424 13.065647311556203 16-17 15.903835218297846 32.611044417767104 31.239021924559296 20.24609843937575 18-19 15.764980190700054 36.83297632362141 33.1030146784701 14.299028807208437 20-21 20.77691496016226 28.80227716453841 30.90536877555018 19.51543909974916 22-23 17.536804195362354 31.28672521640235 30.48840588867126 20.688064699564034 24-25 21.891616091089748 31.241963459211497 31.359174530760797 15.50724591893796 26-27 21.64424070288731 27.458352157663107 36.552793951782334 14.344613187667246 28-29 16.13303760994844 33.42590486300677 35.29881963401071 15.142237893034071 30-31 17.259086973284205 35.055081183913636 31.892100290100554 15.793731552701601 32-33 16.67419606554684 34.84774291788953 31.22762899755795 17.250432019005675 34-35 18.927474693862077 31.28767486825644 31.910692396153213 17.874158041728275 36-37 19.904389670545637 31.601962760629775 34.51513935537279 13.978508213451798 38-39 20.660642633724052 30.923825471549083 34.194180278664184 14.221351616062684 40-41 15.267276869073987 34.287393327793694 34.91265596021573 15.532673842916585 42-43 14.868374491317645 37.32211915173268 29.380355382554406 18.429150974395267 44-45 17.976317601936042 33.680123340557685 29.20207758070529 19.14148147680098 46-47 19.02025394037235 34.39884245145025 27.62130723373066 18.959596374446736 48-49 20.076263770286197 33.63188040425485 29.010877222911986 17.28097860254697 50-51 18.88138399034543 34.581751091509915 32.295424820082516 14.241440098062135 52-53 17.43042457673437 37.258117001077316 29.204459651151403 16.10699877103691 54-55 13.488513154901813 34.57900523163242 30.977835064574016 20.954646548891752 56-57 18.886379073404207 35.06563255248235 27.23174372502251 18.816244649090937 58-59 20.753155259292022 33.08322934272671 26.952469711090398 19.211145686890866 60-61 17.174448726859165 33.074177039236744 31.425412270171226 18.325961963732862 62-63 19.743160422063564 32.30176281038732 29.8562582927908 18.098818474758325 64-65 17.995406046704137 36.06442908913976 29.86954310069731 16.0706217634588 66-67 16.73711678924356 32.11103950261582 29.66171304369803 21.490130664442592 68-69 15.599805393383376 36.60026031794172 28.296623448833625 19.50331083984128 70-71 19.490973885241655 34.63254203446162 25.644654783493298 20.231829296803422 72-73 17.60092767837113 32.08136775685727 29.15519970678286 21.162504857988747 74-75 16.941007721568578 36.497365062113765 27.552161660074052 19.0094655562436 76-77 18.56139109336299 31.896072385381384 28.31376687054542 21.228769650710205 78-79 16.57525244227634 32.332832029522166 29.59008935003223 21.501826178169274 80-81 17.94312865081396 34.81972205636588 26.990670975772336 20.24647831704782 82-83 16.696101631192008 32.38657007364533 28.312855233684132 22.60447306147853 84-85 16.324841408244247 31.70034372077741 28.233123562564767 23.741691308413575 86-87 16.84937132747836 33.391672458457066 27.84671763442219 21.912238579642384 88-89 17.61247500292225 31.561239286402536 26.47368238152254 24.352603329152675 90-91 15.691254940243196 30.285625827322903 29.090173852331354 24.93294538010255 92-93 16.58310666300203 34.25612641728191 26.878836415102086 22.281930504613964 94-95 17.01591273113266 33.68873977614133 25.7298468751086 23.565500617617417 96-97 13.448537309660708 33.15505149428192 27.853035951222594 25.543375244834778 98-99 17.099368796163496 34.51370767490791 24.348419463066044 24.03850406586255 100-101 16.3712816235752 32.82754568200774 24.83666944676119 25.964503247655873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2740.0 1 1806.5 2 842.5 3 791.5 4 704.5 5 755.0 6 1197.0 7 1937.0 8 2450.5 9 2416.0 10 2027.5 11 1725.0 12 1662.5 13 1577.5 14 1415.5 15 1184.0 16 1014.0 17 904.5 18 798.0 19 761.0 20 759.0 21 803.0 22 841.0 23 886.0 24 939.0 25 1042.5 26 1154.0 27 1303.0 28 1435.5 29 1498.0 30 1691.5 31 1731.0 32 1802.5 33 2045.5 34 2200.5 35 2303.5 36 2450.0 37 2631.5 38 2613.5 39 2400.5 40 2178.5 41 2015.5 42 1889.0 43 1824.5 44 1905.5 45 2195.0 46 3611.0 47 8796.5 48 25270.5 49 24335.5 50 8352.0 51 4061.0 52 1729.5 53 1073.5 54 938.5 55 875.0 56 915.5 57 980.5 58 910.5 59 870.5 60 938.0 61 943.5 62 757.5 63 398.5 64 171.0 65 109.5 66 84.5 67 70.0 68 46.0 69 29.5 70 25.0 71 20.0 72 17.0 73 19.5 74 18.5 75 6.5 76 3.0 77 4.0 78 2.5 79 2.0 80 1.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02590509888165793 2 0.006318316800404372 3 0.008845643520566122 4 0.0 5 0.0037909900802426235 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.006950148480444809 20-21 0.0018954950401213117 22-23 0.0 24-25 0.0053705692803437165 26-27 0.0053705692803437165 28-29 0.008845643520566122 30-31 0.030959752321981428 32-33 9.477475200606559E-4 34-35 0.005054653440323497 36-37 0.014848044480950276 38-39 0.009477475200606558 40-41 0.010425222720667215 42-43 0.01137297024072787 44-45 0.005686485120363935 46-47 0.0028432425601819674 48-49 0.0022114108801415304 50-51 0.0018954950401213117 52-53 0.004106905920262842 54-55 0.0012636633600808743 56-57 0.001579579200101093 58-59 0.001579579200101093 60-61 0.0 62-63 0.0 64-65 0.01168888608074809 66-67 0.0012636633600808743 68-69 0.0012636633600808743 70-71 0.004422821760283061 72-73 0.016111707841031147 74-75 0.007581980160485247 76-77 0.0037909900802426235 78-79 0.010109306880646995 80-81 3.159158400202186E-4 82-83 0.007266064320465028 84-85 0.0012636633600808743 86-87 0.0 88-89 9.477475200606559E-4 90-91 0.0022114108801415304 92-93 3.159158400202186E-4 94-95 3.159158400202186E-4 96-97 0.0 98-99 6.318316800404372E-4 100-101 0.0012636633600808743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 158270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.65767359575409 #Duplication Level Percentage of deduplicated Percentage of total 1 83.27388774169326 28.028053326593792 2 9.230336024028533 6.21343274151766 3 2.7726675427069645 2.7996461742591774 4 1.351605030974282 1.8196752385164592 5 0.7133470996808711 1.2004801920768309 6 0.4674300732119392 0.9439565299804131 7 0.33602402853388397 0.7916850950906678 8 0.2665665477754834 0.7177607885259366 9 0.15393279519429323 0.46629177986984266 >10 1.1432325886990802 7.079673974853098 >50 0.11826544021024966 2.8426107285019273 >100 0.10324760653275765 7.691918872812282 >500 0.026281208935611037 6.4750110570544 >1k 0.03942181340341656 25.835597396853476 >5k 0.003754458419373005 7.094206103494029 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6057 3.8270044860049284 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5171 3.2672016174891008 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4432 2.8002780059392176 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3366 2.1267454350161117 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3348 2.1153724647753838 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2674 1.689517912428129 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 1.6250710810640046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2478 1.5656789031402034 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2408 1.5214506855373728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 1.2522903898401467 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1968 1.2434447463195804 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1816 1.1474063309534341 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1661 1.0494724205471662 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1342 0.8479181146142668 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1315 0.8308586592531749 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1305 0.8245403424527706 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1292 0.8163265306122449 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1195 0.7550388576483225 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1189 0.7512478675680799 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1181 0.7461932141277563 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1179 0.7449295507676754 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1138 0.7190244518860176 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1049 0.6627914323624187 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 972 0.614140392999305 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 956 0.604031086118658 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 945 0.5970809376382131 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 890 0.5623301952359891 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 867 0.5477980665950591 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 792 0.5004106905920263 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 741 0.46818727490996404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.43217286914765907 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 668 0.4220635622670121 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 624 0.39426296834523283 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 560 0.35382574082264484 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 525 0.33171163202122955 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 523 0.33044796866114867 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 501 0.31654767170025905 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 490 0.30959752321981426 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 487 0.30770202817969294 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 483 0.3051747014595312 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 463 0.29253806785872244 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 0.2704239590573071 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 415 0.26221014721678143 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 395 0.2495735136159727 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 369 0.23314588993492133 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 335 0.21166361281354648 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 332 0.20976811777342513 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.20597712769318252 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 289 0.18259935553168635 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 281 0.17754470209136286 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 277 0.1750173753712011 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 277 0.1750173753712011 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 272 0.17185821697099893 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 261 0.16490806849055412 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 259 0.16364440513047326 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 244 0.1541669299298667 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 237 0.1497441081695836 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 235 0.14848044480950273 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 227 0.14342579136917927 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 223 0.1408984646490175 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 215 0.135843811208694 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.13331648448853226 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 211 0.13331648448853226 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 209 0.13205282112845138 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 204 0.12889366272824918 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 176 0.11120237568711694 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 175 0.11057054400707653 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 166 0.10488405888671257 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 163 0.10298856384659126 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 162 0.10235673216655082 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.318316800404373E-4 0.0 14-15 0.0 0.0 0.0 0.0012636633600808746 0.0 16-17 0.0 0.0 0.0 0.0012636633600808746 0.0 18-19 0.0 0.0 0.0 0.0022114108801415304 0.0 20-21 0.0 0.0 0.0 0.004738737600303279 0.0 22-23 0.0 0.0 0.0 0.008845643520566122 0.0 24-25 0.0 0.0 0.0 0.015163960320970494 0.0 26-27 0.0 0.0 0.0 0.02938017312188033 0.0 28-29 0.0 0.0 0.0 0.05591710368357869 0.0 30-31 0.0 0.0 0.0 0.0935110886459847 0.0 32-33 0.0 0.0 0.0 0.11594111328742024 0.0 34-35 0.0 0.0 0.0 0.135843811208694 0.0 36-37 0.0 0.0 0.0 0.15511467744992735 0.0 38-39 0.0 0.0 0.0 0.19934289505275793 0.0 40-41 0.0 0.0 0.0 0.27263536993744864 0.0 42-43 0.0 0.0 0.0 0.40152903266569784 0.0 44-45 0.0 0.0 0.0 0.511467744992734 0.0 46-47 0.0 0.0 0.0 0.7957920010109307 0.0 48-49 0.0 0.0 0.0 1.0469450938270044 0.0 50-51 0.0 0.0 0.0 1.1944777911164466 0.0 52-53 0.0 0.0 0.0 1.3559107853667784 0.0 54-55 0.0 0.0 0.0 1.5091299677765844 0.0 56-57 0.0 0.0 0.0 1.8809629114803816 0.0 58-59 0.0 0.0 0.0 2.3737916219119226 0.0 60-61 0.0 0.0 0.0 2.950969861628862 0.0 62-63 0.0 0.0 0.0 3.4832880520629304 0.0 64-65 0.0 0.0 0.0 3.9483161685726924 0.0 66-67 0.0 0.0 0.0 4.609843937575031 0.0 68-69 0.0 0.0 0.0 5.292538067858722 0.0 70-71 0.0 0.0 0.0 5.719972199406079 0.0 72-73 0.0 0.0 0.0 6.2213306375181645 0.0 74-75 0.0 0.0 0.0 6.752385164592153 0.0 76-77 0.0 0.0 0.0 7.2300499147027235 0.0 78-79 0.0 0.0 0.0 7.727301446894547 0.0 80-81 0.0 0.0 0.0 8.42452770581917 0.0 82-83 0.0 0.0 0.0 9.200732924748849 0.0 84-85 0.0 0.0 0.0 10.617931383079547 0.0 86-87 0.0 0.0 0.0 11.895179124281292 0.0 88-89 0.0 0.0 0.0 13.000252732672017 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAACGC 25 0.0016145741 37.997475 78-79 TTGCGTT 75 6.8876943E-6 37.997475 4 TTTGCGT 75 6.8876943E-6 37.997475 3 GGGATGT 50 0.001612134 37.997475 7 CTTTGCG 70 2.0461687E-4 33.926315 2 CCGGGCG 30 0.0039479514 31.66456 60-61 GCACAAG 30 0.0039479514 31.66456 94-95 TGGTATG 30 0.0039479514 31.66456 74-75 GCTTTGC 85 6.3732907E-4 27.948149 1 AGCACAA 35 0.008385268 27.141052 92-93 CATGGGT 70 0.008372821 27.141052 4 AGGAGGG 70 0.008372821 27.141052 5 GGGTAAG 35 0.008385268 27.141052 62-63 AGGACAA 70 0.008372821 27.141052 5 TGGGATG 165 8.3200575E-8 25.907366 6 GAGTACT 1675 0.0 25.378908 12-13 AGTACTT 1660 0.0 24.17761 12-13 GTACTTT 1780 0.0 23.881838 14-15 ATGGGAC 180 1.9302388E-7 23.748419 5 ACTTTTT 1950 0.0 22.286978 16-17 >>END_MODULE