##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937025_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 163273 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04039247150478 33.0 31.0 34.0 31.0 34.0 2 32.05602273492862 33.0 31.0 34.0 30.0 34.0 3 32.0124882864895 33.0 31.0 34.0 30.0 34.0 4 35.57443667967147 37.0 35.0 37.0 33.0 37.0 5 35.5841749707545 37.0 35.0 37.0 33.0 37.0 6 35.77187287549074 37.0 35.0 37.0 35.0 37.0 7 35.77240572538019 37.0 35.0 37.0 35.0 37.0 8 35.76345752206427 37.0 35.0 37.0 35.0 37.0 9 37.34809184617175 39.0 37.0 39.0 35.0 39.0 10-11 37.230439203052555 39.0 37.0 39.0 34.0 39.0 12-13 37.141042915852594 39.0 37.0 39.0 33.5 39.0 14-15 37.994775621198855 40.0 37.5 41.0 33.0 41.0 16-17 38.00118513165067 40.0 37.5 41.0 33.5 41.0 18-19 38.12219411660226 40.0 37.5 41.0 34.0 41.0 20-21 38.08083394070054 40.0 37.5 41.0 34.0 41.0 22-23 37.936887911657166 40.0 37.0 41.0 33.5 41.0 24-25 37.882736276052995 40.0 37.0 41.0 33.5 41.0 26-27 37.58780386224299 40.0 36.5 41.0 33.0 41.0 28-29 37.40087767113975 39.5 36.0 41.0 33.0 41.0 30-31 36.99312501148383 39.0 35.5 41.0 33.0 41.0 32-33 36.464384803366144 38.0 35.0 41.0 32.5 41.0 34-35 36.133788195231304 38.0 35.0 40.0 31.0 41.0 36-37 35.684546740734845 38.0 35.0 40.0 30.5 41.0 38-39 35.29079823363324 38.0 35.0 40.0 30.0 41.0 40-41 34.94717436440808 38.0 35.0 40.0 29.5 41.0 42-43 34.60980382549472 38.0 35.0 40.0 26.5 41.0 44-45 34.090645728320055 37.0 35.0 40.0 23.5 41.0 46-47 33.299672940412684 37.0 33.5 40.0 13.5 41.0 48-49 32.63100451391228 37.0 33.0 40.0 3.5 41.0 50-51 31.677791184090452 36.0 32.0 39.0 2.0 40.5 52-53 31.74781500921769 36.5 32.5 39.0 2.0 40.0 54-55 32.093371224881025 37.0 33.0 40.0 2.0 41.0 56-57 31.88200437304392 36.5 33.0 40.0 2.0 41.0 58-59 31.63351870793089 36.0 32.5 40.0 2.0 41.0 60-61 31.120029031131907 35.5 31.5 39.5 2.0 41.0 62-63 30.714135221377692 35.0 31.0 39.0 2.0 41.0 64-65 30.175301488917334 35.0 30.0 39.0 2.0 40.5 66-67 29.63102595040209 35.0 29.0 38.5 2.0 40.0 68-69 29.22989410374036 35.0 29.0 37.0 2.0 39.0 70-71 28.573181113840008 35.0 27.0 37.0 2.0 39.0 72-73 27.993826290936042 35.0 26.0 36.0 2.0 38.5 74-75 27.570195317045684 34.0 26.0 35.5 2.0 37.0 76-77 27.068814194631077 34.0 25.5 35.0 2.0 37.0 78-79 26.59881915564729 34.0 24.5 35.0 2.0 36.0 80-81 26.112357830137256 34.0 21.0 35.0 2.0 36.0 82-83 25.68738248210053 33.5 19.0 35.0 2.0 35.5 84-85 25.27887648294574 33.0 16.0 35.0 2.0 35.0 86-87 24.92124539881058 33.0 7.5 35.0 2.0 35.0 88-89 24.355603804670707 32.5 2.0 35.0 2.0 35.0 90-91 23.779415457546563 32.0 2.0 35.0 2.0 35.0 92-93 23.472904276885952 32.0 2.0 35.0 2.0 35.0 94-95 23.139793474732503 32.0 2.0 35.0 2.0 35.0 96-97 22.500692092385144 31.0 2.0 35.0 2.0 35.0 98-99 21.820010044526654 31.0 2.0 35.0 2.0 35.0 100-101 19.897487643394804 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08945179926145741 1101 2 0.00778265566904679 1101 3 -0.03315210470759311 1101 4 0.07742085780498797 1101 5 -0.03385807124442408 1101 6 -0.06563812365902066 1101 7 -0.24811935567293375 1101 8 -0.14853251930583866 1101 9 0.07826596374432881 1101 10-11 0.14949491588451025 1101 12-13 0.08489576285598588 1101 14-15 0.1263223465562291 1101 16-17 0.14857084081677385 1101 18-19 0.2908183731973395 1101 20-21 0.4760826196262613 1101 22-23 0.35651412860109843 1101 24-25 0.3344392756423389 1101 26-27 0.6135315768135143 1101 28-29 0.5267289107871775 1101 30-31 0.7697331822497304 1101 32-33 0.9044937714369112 1101 34-35 1.3781464931279785 1101 36-37 1.2910480350375977 1101 38-39 1.2415616374527616 1101 40-41 1.524714331956588 1101 42-43 1.6737826802761901 1101 44-45 1.7757570756454228 1101 46-47 2.1039082771573234 1101 48-49 2.111213717288024 1101 50-51 2.090666992159065 1101 52-53 2.2201621547810646 1101 54-55 2.223382387130698 1101 56-57 2.3471694472239157 1101 58-59 2.412724382464301 1101 60-61 2.864643382356917 1101 62-63 2.5547723503367585 1101 64-65 2.4706036767415007 1101 66-67 2.665220805035176 1101 68-69 3.0185498443035605 1101 70-71 2.513331798119502 1101 72-73 2.8375739218868112 1101 74-75 2.532776370040594 1101 76-77 2.8632158969932 1101 78-79 3.084392175203032 1101 80-81 3.117942653443844 1101 82-83 3.0932228788083727 1101 84-85 2.861835363093281 1101 86-87 2.7785095239395368 1101 88-89 2.6866531713763955 1101 90-91 2.5821039450285994 1101 92-93 2.648164772773086 1101 94-95 2.429570093530966 1101 96-97 2.1758465061035572 1101 98-99 1.4797299266587167 1101 100-101 1.5298351856084942 1102 1 0.2411567659034155 1102 2 0.046638598342632065 1102 3 -0.005230187237863504 1102 4 -0.17737281652167525 1102 5 0.20046364854801624 1102 6 -0.1722850225965047 1102 7 2.1274217732525358E-4 1102 8 0.3360857164243356 1102 9 -0.10694530386130197 1102 10-11 0.07167801447606337 1102 12-13 0.031028700834731637 1102 14-15 -0.16385556300640758 1102 16-17 0.22989651415504397 1102 18-19 0.4434746618061851 1102 20-21 0.4917670772492002 1102 22-23 0.06326604359986021 1102 24-25 0.23805306363343703 1102 26-27 0.2831448706113946 1102 28-29 0.38559967925765193 1102 30-31 0.7000519368827867 1102 32-33 0.887864169262464 1102 34-35 1.1346698437580756 1102 36-37 1.1284152206071525 1102 38-39 1.2426056206501954 1102 40-41 1.4585418585194674 1102 42-43 1.708734496436307 1102 44-45 1.4990644638342019 1102 46-47 2.1494420058452377 1102 48-49 2.546838871723409 1102 50-51 2.1231910840790036 1102 52-53 2.184555532591787 1102 54-55 2.268187180805022 1102 56-57 2.594012787970147 1102 58-59 2.6148494133513758 1102 60-61 2.387042690486396 1102 62-63 2.9074101066994977 1102 64-65 2.9686825005616164 1102 66-67 3.164010031622766 1102 68-69 3.1031310155415177 1102 70-71 3.1819072861353135 1102 72-73 2.924280124011844 1102 74-75 3.169686426872815 1102 76-77 3.0893401865904337 1102 78-79 2.910176706954946 1102 80-81 2.7590657075579976 1102 82-83 2.6959162319094325 1102 84-85 2.854793109572153 1102 86-87 3.0374173569022282 1102 88-89 3.3077737545244013 1102 90-91 2.950963594151407 1102 92-93 2.4861620547103236 1102 94-95 2.6474351787793005 1102 96-97 2.901254215517941 1102 98-99 2.5490775915957045 1102 100-101 2.73698863260627 1103 1 -0.6728703150371622 1103 2 -0.4350303855176101 1103 3 -0.2290710867119614 1103 4 -0.05902971067629892 1103 5 -0.37521790860606785 1103 6 -0.32727504858937095 1103 7 -0.2594136859191494 1103 8 -0.13204279714636158 1103 9 -0.1371846800324903 1103 10-11 -0.13891710128287116 1103 12-13 0.04193966015287032 1103 14-15 0.16131218165901373 1103 16-17 0.11431137240325029 1103 18-19 0.18965129240523027 1103 20-21 0.12255502381270134 1103 22-23 -0.408240031477213 1103 24-25 -0.126640462329199 1103 26-27 -0.1210215441864051 1103 28-29 -0.30673242495560515 1103 30-31 0.25019399092377625 1103 32-33 0.382665595847719 1103 34-35 0.44611577254971024 1103 36-37 0.6246900800643189 1103 38-39 0.6854137570220686 1103 40-41 0.6331474318737662 1103 42-43 0.5800849165535809 1103 44-45 0.897231362220225 1103 46-47 0.818252076570019 1103 48-49 1.3464595572097124 1103 50-51 0.8559708096426917 1103 52-53 1.0169922127586233 1103 54-55 0.8507523140940307 1103 56-57 1.0033173276546101 1103 58-59 1.176597584783405 1103 60-61 1.0182613328124575 1103 62-63 0.942130230014488 1103 64-65 1.2265181410137664 1103 66-67 0.888908780338241 1103 68-69 1.2979263972739723 1103 70-71 1.4511979226583165 1103 72-73 1.1979910591505707 1103 74-75 1.354182890633922 1103 76-77 1.1825442608212278 1103 78-79 0.8770358014359942 1103 80-81 1.335132624078586 1103 82-83 1.2974093105529683 1103 84-85 0.8639812858400617 1103 86-87 1.1885991256261583 1103 88-89 0.9300384586288573 1103 90-91 0.9611567271986168 1103 92-93 1.2213143850010795 1103 94-95 0.9915082006012206 1103 96-97 0.1428896504451025 1103 98-99 -0.5669382459186494 1103 100-101 -0.19015577892730917 1104 1 -0.33346840447368464 1104 2 -0.19934807438188784 1104 3 -0.26942827901432764 1104 4 -0.012788536648891125 1104 5 -0.15111896541533554 1104 6 -0.10587581466072038 1104 7 -0.36583299460898644 1104 8 -0.45215447742694437 1104 9 -0.3539921007717979 1104 10-11 -0.3768491701369925 1104 12-13 -0.2673962575175324 1104 14-15 -0.3233663914177356 1104 16-17 -0.2946945808017887 1104 18-19 -0.0232450110697755 1104 20-21 0.2528794504129124 1104 22-23 -0.21049209663942037 1104 24-25 0.30220950767402854 1104 26-27 0.43260345004498646 1104 28-29 0.2821618479688439 1104 30-31 0.4006047159226256 1104 32-33 0.13536530510980072 1104 34-35 0.224439645363411 1104 36-37 0.2744662580143924 1104 38-39 0.4988338502428036 1104 40-41 0.6886645300324261 1104 42-43 1.1184346327379941 1104 44-45 0.9639857117518176 1104 46-47 0.699211050219013 1104 48-49 0.31571286839158574 1104 50-51 0.09576037076687527 1104 52-53 0.15233532965373087 1104 54-55 0.09021883308429324 1104 56-57 0.633445621530651 1104 58-59 0.6436242126850154 1104 60-61 0.39571299186455633 1104 62-63 0.7524237368675983 1104 64-65 0.6909488946249134 1104 66-67 1.0090804541579814 1104 68-69 1.040507965412786 1104 70-71 0.9846673951766611 1104 72-73 1.180754453861912 1104 74-75 1.115373993130575 1104 76-77 0.9073585115942215 1104 78-79 1.1045959103473422 1104 80-81 0.5712533495389174 1104 82-83 1.2401385550958643 1104 84-85 0.35914719105026904 1104 86-87 0.8750290485575327 1104 88-89 0.589482826158509 1104 90-91 0.6834904758718388 1104 92-93 0.17306573485571164 1104 94-95 0.35427297977884464 1104 96-97 0.27296025822580106 1104 98-99 0.6505844824029161 1104 100-101 0.5584712920035138 1105 1 0.3287706847103742 1105 2 0.25504787754726976 1105 3 0.29584686330066035 1105 4 0.39764789598868333 1105 5 0.554067625350001 1105 6 0.18090470399835823 1105 7 0.16085897250044212 1105 8 -0.1506997019683709 1105 9 0.32532478727374325 1105 10-11 0.29655373941392327 1105 12-13 0.05961196512636491 1105 14-15 0.5192016035221769 1105 16-17 0.5844325539230653 1105 18-19 0.35113829063799784 1105 20-21 0.1334613605963355 1105 22-23 0.28897026895197087 1105 24-25 0.41694701558041913 1105 26-27 0.26100319704055863 1105 28-29 0.22213654752608392 1105 30-31 -0.25255783942209575 1105 32-33 0.13612431839253247 1105 34-35 0.2725737377100259 1105 36-37 0.5405320391655621 1105 38-39 1.0656586446767093 1105 40-41 0.652990905743998 1105 42-43 0.25334924374368484 1105 44-45 -0.3611882287921446 1105 46-47 -0.5953177290346261 1105 48-49 -0.42103602471404145 1105 50-51 -0.4226457013393876 1105 52-53 -0.12932184934690127 1105 54-55 0.30176216009251533 1105 56-57 -0.10045697176473212 1105 58-59 -0.25254909534787373 1105 60-61 -0.5555836558267764 1105 62-63 -0.40905633903372873 1105 64-65 0.025041241240980838 1105 66-67 -0.39091954584201716 1105 68-69 0.10043838919520098 1105 70-71 0.25848143692239134 1105 72-73 0.42771840073098133 1105 74-75 0.6944378767485411 1105 76-77 0.7350941219674034 1105 78-79 0.8321733929532904 1105 80-81 0.7507599909051414 1105 82-83 0.8253694216360294 1105 84-85 0.600070657210928 1105 86-87 0.33692025665367353 1105 88-89 0.10017226322365858 1105 90-91 0.04949300591611561 1105 92-93 -0.028357415049413248 1105 94-95 0.5205917653575938 1105 96-97 0.23652762065464472 1105 98-99 -0.04147750368184333 1105 100-101 0.38649153235772005 1106 1 0.13024127294567123 1106 2 0.2621686205813276 1106 3 0.4975496496783691 1106 4 0.22829805078744414 1106 5 0.5832471919134719 1106 6 0.3602390693234341 1106 7 0.38345959169548394 1106 8 0.41067800700995605 1106 9 0.40852912163907007 1106 10-11 0.5323896527266214 1106 12-13 0.6068255874173829 1106 14-15 0.38553287287201954 1106 16-17 0.6112901390623833 1106 18-19 0.4166955661797971 1106 20-21 0.6697229705034573 1106 22-23 0.7277860584256572 1106 24-25 0.6291761177475976 1106 26-27 0.45980350663808167 1106 28-29 0.7817727704363122 1106 30-31 0.7434173927760455 1106 32-33 0.6733534205597138 1106 34-35 0.5584096510227141 1106 36-37 0.741925228020051 1106 38-39 0.3266493567510125 1106 40-41 0.32098626178115097 1106 42-43 0.33628268027619157 1106 44-45 0.287379882662961 1106 46-47 0.01443459294679883 1106 48-49 0.0943277523757402 1106 50-51 -0.18235932363040774 1106 52-53 0.07257443548282083 1106 54-55 0.2455315099377131 1106 56-57 0.5594501489783035 1106 58-59 -0.6651264947286784 1106 60-61 -0.6546548632571145 1106 62-63 -0.4397451935228922 1106 64-65 -0.5068086039602555 1106 66-67 -1.0764458616314911 1106 68-69 -1.002502787275386 1106 70-71 -1.0792120615296206 1106 72-73 -0.7902769553061724 1106 74-75 -0.7477060860997575 1106 76-77 -0.4284507696734643 1106 78-79 -0.31670431602504223 1106 80-81 0.04228475870700166 1106 82-83 0.07304744021188014 1106 84-85 -0.3848751632225067 1106 86-87 -0.4453939848323927 1106 88-89 -0.2510661268692225 1106 90-91 -0.4602206163749081 1106 92-93 0.24575249207133254 1106 94-95 0.19733325142570735 1106 96-97 0.37387282189303406 1106 98-99 0.7224930845534523 1106 100-101 1.290361501397971 1107 1 -0.33503650483210734 1107 2 0.05602826970413588 1107 3 -0.1296433327777713 1107 4 -0.29728674453419757 1107 5 -0.21317093674149845 1107 6 -0.1112521587467441 1107 7 0.1422315215200456 1107 8 -0.15511146667425635 1107 9 -0.1064562584778912 1107 10-11 0.14185602699562594 1107 12-13 -0.17241417866448927 1107 14-15 -0.1298180043209669 1107 16-17 0.08680183496882421 1107 18-19 0.08573796384061438 1107 20-21 0.3897522102695419 1107 22-23 0.22577582450752942 1107 24-25 -0.010626513831347495 1107 26-27 0.1412728048836982 1107 28-29 0.43729177628426186 1107 30-31 0.5140679775713721 1107 32-33 0.3043841223141044 1107 34-35 -0.12031841915271713 1107 36-37 0.013489555505429962 1107 38-39 0.04923707604925909 1107 40-41 0.17880789920805285 1107 42-43 0.42802244635806375 1107 44-45 0.6967366078091572 1107 46-47 0.8806626631222372 1107 48-49 1.404086524305562 1107 50-51 0.9441026646736859 1107 52-53 1.1171650787576723 1107 54-55 1.1689598725108112 1107 56-57 1.774910675294091 1107 58-59 1.4467156105344792 1107 60-61 1.9129620958072096 1107 62-63 1.7980106544303212 1107 64-65 1.89816215627366 1107 66-67 2.1028304541579814 1107 68-69 1.716247212724614 1107 70-71 1.2733098682949375 1107 72-73 1.1852347406002615 1107 74-75 1.3794868963563793 1107 76-77 1.595306540268055 1107 78-79 1.5963073798813916 1107 80-81 1.6605230628005678 1107 82-83 1.2720605981066164 1107 84-85 1.6535020297599452 1107 86-87 1.9813238514249178 1107 88-89 1.8057686684524157 1107 90-91 1.982213594151407 1107 92-93 1.6742978137087583 1107 94-95 1.9864414385602096 1107 96-97 0.3163070324193491 1107 98-99 0.024576417886787993 1107 100-101 0.35450623824007366 1108 1 0.10453205308751379 1108 2 0.24219848247009423 1108 3 0.04411553247045674 1108 4 -0.05053733555074302 1108 5 0.2911139332348611 1108 6 0.16534358593411014 1108 7 -0.06521528699059331 1108 8 0.43265449077254914 1108 9 0.862663126864895 1108 10-11 0.14311193716110893 1108 12-13 0.2483896038414244 1108 14-15 0.6574160382322276 1108 16-17 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-0.6337830989497633 2316 72-73 -1.0760048226624228 2316 74-75 -0.9248242789808039 2316 76-77 -1.3531921752104275 2316 78-79 -1.4557558828032562 2316 80-81 -1.2384613623760536 2316 82-83 -0.7711113672113008 2316 84-85 -0.4763149259240613 2316 86-87 -0.60680636694374 2316 88-89 -0.7896271247396207 2316 90-91 -0.9052502786809633 2316 92-93 -1.8661846846855923 2316 94-95 -1.3285071805791624 2316 96-97 -1.7619082854997252 2316 98-99 -1.8681861061979923 2316 100-101 -0.9260212184073247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 415.0 3 121.0 4 122.0 5 89.0 6 126.0 7 156.0 8 228.0 9 391.0 10 620.0 11 885.0 12 1168.0 13 1479.0 14 1702.0 15 2231.0 16 2869.0 17 4006.0 18 3395.0 19 1983.0 20 1877.0 21 1932.0 22 1997.0 23 2020.0 24 2163.0 25 2287.0 26 2433.0 27 2704.0 28 3067.0 29 3486.0 30 4168.0 31 5154.0 32 6279.0 33 8406.0 34 11810.0 35 19714.0 36 21402.0 37 23876.0 38 14621.0 39 1891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.05251549979171 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37.69637355839606 18.35453504253612 44-45 15.27258027965432 28.734389641888125 36.39487239163854 19.59815768681901 46-47 16.744960893717884 27.831300949942733 34.655760597281855 20.76797755905753 48-49 17.01725642713868 26.67503713117641 35.814971902800536 20.49273453888438 50-51 17.18716505484679 27.79853374409731 38.50116063280518 16.51314056825072 52-53 15.688496225635056 29.151265522362923 36.46541824250869 18.69482000949333 54-55 13.646330334229596 26.08944638055748 39.210882520472104 21.05334076474083 56-57 17.569959515657825 28.62812589956698 33.571686684265 20.23022790051019 58-59 17.650913812533688 26.430128864716547 35.11624773384291 20.802709588906854 60-61 17.084636138409195 24.437203519334595 38.66643800587373 19.811722336382484 62-63 19.9446933481961 25.08000477731876 36.21391363465148 18.76138823983365 64-65 18.07755793264593 27.23469803364335 37.945691685321684 16.742052348389034 66-67 16.91187730076381 25.70332167510918 36.810077115783926 20.57472390834308 68-69 15.288752496110355 28.578166538032757 37.26922464380658 18.863856322050303 70-71 17.74347193506394 27.47438548127728 33.22520866835133 21.55693391530745 72-73 15.848761887260531 25.301297070488964 35.71982052342231 23.130120518828196 74-75 16.038432206115218 28.09918620998049 35.86434178568256 19.99803979822173 76-77 17.82326219670956 24.519008396102443 35.82939462906749 21.82833477812051 78-79 16.190735811665537 24.487033015501574 38.82009170462168 20.502139468211208 80-81 17.586686519242907 27.103668437467448 35.99964461437596 19.310000428913686 82-83 15.758533753626253 25.7787825596819 36.51861450378171 21.94406918291013 84-85 16.510563746960127 24.547158600158042 35.01411970817228 23.92815794470955 86-87 18.03583952761163 25.197007145241663 35.876892355847126 20.890260971299586 88-89 19.189846434675015 23.135256319945118 34.51146380158405 23.163433443795824 90-91 16.87168428919737 23.166174542692268 37.39049731067521 22.571643857435156 92-93 17.2941198093441 26.96476051609425 36.37992721747761 19.36119245708404 94-95 16.982172225914436 27.74376517933436 34.277042567983514 20.997020026767693 96-97 14.439822840795625 26.563160441795358 34.42383440637577 24.573182311033246 98-99 17.61950854923391 26.736649288431728 32.17587345540982 23.46796870692454 100-101 19.952636690327896 24.458891036815324 33.94993459206588 21.6385376807909 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2112.0 1 1466.5 2 931.5 3 941.5 4 676.5 5 637.5 6 1179.5 7 2443.5 8 3956.5 9 4178.0 10 2688.0 11 1449.5 12 1267.0 13 1216.0 14 1110.0 15 1018.0 16 909.0 17 878.5 18 899.0 19 900.5 20 902.0 21 914.0 22 977.0 23 1022.5 24 1052.5 25 1134.0 26 1264.5 27 1397.5 28 1518.5 29 1644.0 30 1724.0 31 1752.0 32 1870.5 33 2007.0 34 2112.5 35 2259.0 36 2386.5 37 2536.0 38 2513.5 39 2270.5 40 2072.0 41 1927.0 42 1841.0 43 1839.0 44 1926.0 45 2283.5 46 4068.0 47 9653.5 48 26232.0 49 25428.0 50 8437.5 51 3390.5 52 1661.5 53 1211.0 54 1058.5 55 942.0 56 888.0 57 830.0 58 761.0 59 716.0 60 689.5 61 695.5 62 624.0 63 388.0 64 186.5 65 123.5 66 95.0 67 66.0 68 37.0 69 22.5 70 18.0 71 18.0 72 19.5 73 17.0 74 12.5 75 11.5 76 8.5 77 3.5 78 4.0 79 4.0 80 2.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02756120117839447 2 0.011636951608655442 3 0.010412009334060132 4 0.02388637435460854 5 0.05328498894489597 6 0.04226050847353818 7 0.02021154753082261 8 0.050222633258407694 9 0.028173672315692122 10-11 0.022661432080013227 12-13 0.035523325963263984 14-15 0.02143648980541792 16-17 0.017149191844334336 18-19 0.01684295627568551 20-21 0.009187067059464822 22-23 0.007962124784869512 24-25 6.124711372976549E-4 26-27 0.0 28-29 0.0018374134118929646 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.0623556864882743E-4 50-51 0.0 52-53 3.0623556864882743E-4 54-55 0.0012249422745953097 56-57 0.0 58-59 6.124711372976549E-4 60-61 0.002143648980541792 62-63 9.187067059464823E-4 64-65 9.187067059464823E-4 66-67 0.007349653647571858 68-69 0.011636951608655442 70-71 0.021742725374066747 72-73 0.012555658314601923 74-75 0.015005542863792543 76-77 0.02603002333515033 78-79 0.019292840824876128 80-81 0.04287297961083584 82-83 0.03276720584542454 84-85 0.015924249569739025 86-87 0.01071824490270896 88-89 0.012249422745953096 90-91 0.02143648980541792 92-93 0.028786143452989778 94-95 0.010105773765411306 96-97 0.018374134118929644 98-99 0.025723787766501506 100-101 0.04072933063029405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 163273.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.46603541308116 #Duplication Level Percentage of deduplicated Percentage of total 1 84.41463369996889 28.25023120785433 2 8.766311011877528 5.8674734953115335 3 2.637213813802822 2.647712726537762 4 1.2463168682857195 1.6683713779988119 5 0.6313940081623689 1.0565127118384545 6 0.4245895938946945 0.8525598231183356 7 0.27451913398363864 0.6430946941625376 8 0.22876594498636554 0.6124711372976549 9 0.15190058747094673 0.4575159395613482 >10 0.9534964587031717 6.184733544431718 >50 0.10065701579400083 2.2930919380424197 >100 0.08601599531487344 5.839912294133139 >500 0.04575318899727311 10.682109105608397 >1k 0.03660255119781849 29.45741181946801 >5k 0.0018301275598909244 3.4867981846355494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5693 3.4867981846355494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3977 2.4357977130327733 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3946 2.416811107776546 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3856 2.361688705419757 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3228 1.9770568311968297 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3151 1.9298965536249106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3147 1.92744666907572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3062 1.8753866224054192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2905 1.7792286538496873 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2789 1.7081820019231593 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2419 1.481567681123027 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2133 1.3064009358558977 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2092 1.2812896192266938 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2081 1.2745524367164198 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1791 1.0969358069000998 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1735 1.062637423211431 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1429 0.8752212551983487 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1234 0.7557893834253061 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1090 0.6675935396544438 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1025 0.6277829157300963 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1006 0.6161459641214408 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 995 0.6094087816111666 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 971 0.5947094743160228 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 900 0.5512240235678894 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 876 0.5365247162727457 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 842 0.5157006976046253 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 838 0.5132508130554347 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 836 0.5120258707808394 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 803 0.49181432325001684 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 728 0.4458789879526927 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 718 0.43975427657971616 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 713 0.4366919208932279 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 684 0.4189302579115959 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 683 0.4183177867742982 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 656 0.40178106606726155 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 635 0.38891917218401084 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 614 0.3760572783007601 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 575 0.3521709039461515 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 571 0.34972101939696093 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 568 0.34788360598506796 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 566 0.34665866371047266 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 564 0.34543372143587736 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 535 0.32767205845424535 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 535 0.32767205845424535 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 533 0.32644711617965005 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 502 0.30746051092342275 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 440 0.26948730041096813 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 0.2621376467633963 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 426 0.260912704488801 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 396 0.24253857036987134 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 359 0.21987713828985808 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 359 0.21987713828985808 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 297 0.1819039277774035 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.17945404322821287 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 290 0.1776166298163199 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 288 0.1763916875417246 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 271 0.16597967820766446 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 260 0.15924249569739024 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 243 0.14883048636333013 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 232 0.14209330385305594 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 231 0.1414808327157583 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 223 0.13658106361737704 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.13229376565629344 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 208 0.1273939965579122 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 199 0.12188175632223332 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 190 0.11636951608655441 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 188 0.11514457381195911 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 184 0.11269468926276849 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176 0.10779492016438726 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.10718244902708961 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 166 0.1016702087914107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0012249422745953097 0.0 18-19 0.0 0.0 0.0 0.0012249422745953097 0.0 20-21 0.0 0.0 0.0 0.0024498845491906195 0.0 22-23 0.0 0.0 0.0 0.005206004667030067 0.0 24-25 0.0 0.0 0.0 0.015924249569739028 0.0 26-27 0.0 0.0 0.0 0.023580138785959714 0.0 28-29 0.0 0.0 0.0 0.04072933063029405 0.0 30-31 0.0 0.0 0.0 0.06890300294598617 0.0 32-33 0.0 0.0 0.0 0.08850207933951112 0.0 34-35 0.0 0.0 0.0 0.10687621345844077 0.0 36-37 0.0 0.0 0.0 0.13015011667575166 0.0 38-39 0.0 0.0 0.0 0.16199861581522973 0.0 40-41 0.0 0.0 0.0 0.23273903217310887 0.0 42-43 0.0 0.0 0.0 0.35308961065209804 0.0 44-45 0.0 0.0 0.0 0.45322864160026455 0.0 46-47 0.0 0.0 0.0 0.6985233320879753 0.0 48-49 0.0 0.0 0.0 0.9551487386156927 0.0 50-51 0.0 0.0 0.0 1.0892799176838792 0.0 52-53 0.0 0.0 0.0 1.2264734524385539 0.0 54-55 0.0 0.0 0.0 1.3835722991554023 0.0 56-57 0.0 0.0 0.0 1.760548284162109 0.0 58-59 0.0 0.0 0.0 2.2606309677656444 0.0 60-61 0.0 0.0 0.0 2.819817116118403 0.0 62-63 0.0 0.0 0.0 3.303975550152199 0.0 64-65 0.0 0.0 0.0 3.742198648888671 0.0 66-67 0.0 0.0 0.0 4.371512742462011 0.0 68-69 0.0 0.0 0.0 4.993783417956429 0.0 70-71 0.0 0.0 0.0 5.418225916103704 0.0 72-73 0.0 0.0 0.0 5.909734003785072 0.0 74-75 0.0 0.0 0.0 6.455139551548633 0.0 76-77 0.0 0.0 0.0 6.924904913855935 0.0 78-79 0.0 0.0 0.0 7.403551107654051 0.0 80-81 0.0 0.0 0.0 8.075431945269578 0.0 82-83 0.0 0.0 0.0 8.833364977675426 0.0 84-85 0.0 0.0 0.0 10.225818108321645 0.0 86-87 0.0 0.0 0.0 11.511701261078072 0.0 88-89 0.0 0.0 0.0 12.583525751348967 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGGGA 20 0.0020708805 71.25 1 ACTACCT 30 1.2978981E-4 63.333336 4 TGTTACA 40 7.516619E-6 59.375004 2 CACTACC 35 2.7808457E-4 54.285717 3 GTCACTA 40 5.374523E-4 47.500004 1 GATATGA 40 5.374523E-4 47.500004 2 TCACTAC 40 5.374523E-4 47.500004 2 GATTGGC 40 5.374523E-4 47.500004 9 GGGATGG 60 1.4843663E-6 47.5 7 GGATATC 45 9.600789E-4 42.22222 8 TGGGACT 95 1.9108484E-8 40.0 6 TGGGATG 145 0.0 39.310345 6 GGGACTC 50 0.0016117566 38.000004 7 GGGATAT 50 0.0016117566 38.000004 7 GGAGGCA 25 0.0016141213 38.000004 20-21 ATGGGAC 145 1.6370905E-11 36.03448 5 CATGGGT 80 1.0719596E-5 35.625 4 ATATGAG 55 0.0025731742 34.545456 3 GGATATG 60 0.0039411164 31.666668 1 AACGCCG 30 0.003946847 31.666668 92-93 >>END_MODULE