##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937025_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 163273 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.995406466470268 31.0 31.0 34.0 31.0 34.0 2 32.03218535826499 33.0 31.0 34.0 30.0 34.0 3 32.1063862365486 33.0 31.0 34.0 30.0 34.0 4 35.74907057504915 37.0 35.0 37.0 35.0 37.0 5 35.699007184286444 37.0 35.0 37.0 33.0 37.0 6 35.8886037495483 37.0 35.0 37.0 35.0 37.0 7 35.83542900540812 37.0 35.0 37.0 35.0 37.0 8 35.86879030825672 37.0 35.0 37.0 35.0 37.0 9 37.43122867834853 39.0 37.0 39.0 34.0 39.0 10-11 37.338981338004444 39.0 37.0 39.0 34.0 39.0 12-13 37.25252491226351 39.0 37.0 39.0 34.0 39.0 14-15 37.95199451225861 39.5 37.0 41.0 33.0 41.0 16-17 38.038956226687816 39.0 37.0 41.0 33.5 41.0 18-19 38.34933516258046 40.0 37.0 41.0 34.0 41.0 20-21 38.41631194379965 40.0 37.5 41.0 34.0 41.0 22-23 38.34904730114593 40.0 37.5 41.0 34.0 41.0 24-25 38.09941937736184 40.0 37.0 41.0 33.5 41.0 26-27 37.61485365002174 39.5 36.0 41.0 33.0 41.0 28-29 37.46223502967423 39.0 36.0 41.0 33.0 41.0 30-31 37.37316641453272 39.0 35.5 41.0 33.0 41.0 32-33 36.985579367072326 39.0 35.0 41.0 33.0 41.0 34-35 36.692245502930675 38.0 35.0 41.0 32.5 41.0 36-37 36.30965009523926 38.0 35.0 40.0 31.5 41.0 38-39 35.93257917720628 38.0 35.0 40.0 30.5 41.0 40-41 35.6510139459678 38.0 35.0 40.0 30.0 41.0 42-43 35.346836280340284 38.0 35.0 40.0 30.0 41.0 44-45 34.90423095061645 38.0 35.0 40.0 27.0 41.0 46-47 34.243031609635395 38.0 34.0 40.0 23.5 41.0 48-49 33.608477825482474 37.0 33.0 40.0 20.0 41.0 50-51 33.15979984443233 37.0 33.0 40.0 14.0 41.0 52-53 32.72838742474261 37.0 33.0 40.0 8.5 41.0 54-55 32.51420014331825 37.0 33.0 40.0 3.5 41.0 56-57 32.16974637570205 36.0 32.5 40.0 2.0 41.0 58-59 31.839149767567203 36.0 32.0 39.0 2.0 41.0 60-61 31.19412272696649 35.0 31.0 39.0 2.0 40.0 62-63 30.742795808247536 35.0 31.0 38.5 2.0 40.0 64-65 30.16123915160498 35.0 29.5 38.0 2.0 40.0 66-67 29.670527276402098 35.0 29.0 37.0 2.0 39.0 68-69 29.368493259755134 35.0 29.0 37.0 2.0 39.0 70-71 28.722553637159848 34.0 28.5 36.0 2.0 38.5 72-73 28.073867081513782 34.0 27.0 35.0 2.0 37.0 74-75 27.566247940565802 33.5 26.0 35.0 2.0 37.0 76-77 26.111889289717222 31.5 25.0 34.0 2.0 35.0 78-79 26.782073582282436 33.0 25.5 35.0 2.0 36.0 80-81 26.509983279537952 33.0 25.0 35.0 2.0 36.0 82-83 26.18407207560344 33.0 24.5 35.0 2.0 35.0 84-85 25.888205643309057 33.0 23.5 35.0 2.0 35.0 86-87 25.539357395282746 33.0 20.0 35.0 2.0 35.0 88-89 24.953562438370092 32.0 18.0 35.0 2.0 35.0 90-91 24.302784293790154 31.0 9.0 34.0 2.0 35.0 92-93 23.93584364836807 31.0 2.0 34.0 2.0 35.0 94-95 23.316953813551535 31.0 2.0 34.0 2.0 35.0 96-97 22.448537112688566 31.0 2.0 34.0 2.0 35.0 98-99 21.693133586079753 30.5 2.0 34.0 2.0 35.0 100-101 18.73510929547445 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.005787734204421469 1101 2 -0.04993609629048734 1101 3 -0.05581077404873014 1101 4 0.22833870984189986 1101 5 0.004739840344264223 1101 6 0.06487236433180499 1101 7 0.10364936580513273 1101 8 0.03672247191634881 1101 9 0.18035129561533836 1101 10-11 0.0603680312500785 1101 12-13 0.29747959261817414 1101 14-15 0.1750575134293797 1101 16-17 0.11496592416256846 1101 18-19 0.29836023551209223 1101 20-21 0.334129332098243 1101 22-23 0.3500520939760392 1101 24-25 0.4471928301305468 1101 26-27 0.22582606988626708 1101 28-29 0.3387534409888886 1101 30-31 0.1383385383339899 1101 32-33 0.4293660002052846 1101 34-35 0.4013873147758247 1101 36-37 0.5007764533589167 1101 38-39 0.13967088778293402 1101 40-41 0.2551496519532108 1101 42-43 0.6609253748235275 1101 44-45 0.966060204796193 1101 46-47 1.1893991909596977 1101 48-49 1.0730423468221844 1101 50-51 0.8290829213319952 1101 52-53 1.025887039669655 1101 54-55 1.2625319320764987 1101 56-57 1.4688245965740805 1101 58-59 1.4659249359868447 1101 60-61 1.6279527537743697 1101 62-63 1.7052434745564895 1101 64-65 1.3959281136714203 1101 66-67 1.6471146521509468 1101 68-69 1.3167508679334183 1101 70-71 1.8007796627377353 1101 72-73 2.0915565702801864 1101 74-75 2.22424969594449 1101 76-77 2.078936800117436 1101 78-79 2.2285740616155856 1101 80-81 2.5560191996663697 1101 82-83 2.532177423874238 1101 84-85 2.3657126199880274 1101 86-87 2.228140053667584 1101 88-89 2.1474276498366542 1101 90-91 2.9939409490672446 1101 92-93 3.142425529827449 1101 94-95 2.1653701222721544 1101 96-97 2.3957185085790407 1101 98-99 2.7350444232495015 1101 100-101 2.78923015643759 1102 1 0.048028900500444394 1102 2 0.052053031458463295 1102 3 -0.05219080864225134 1102 4 0.10850920650609908 1102 5 -0.08426436029818518 1102 6 -0.17117903176406912 1102 7 -0.3542206613754928 1102 8 0.09622333675449823 1102 9 -0.07826002140961918 1102 10-11 -0.040484452070899124 1102 12-13 -0.18308625862966466 1102 14-15 -0.18716141417131382 1102 16-17 -0.14169332960565129 1102 18-19 -0.4178863669094568 1102 20-21 -0.10353436940905425 1102 22-23 0.20797154047961897 1102 24-25 0.18127363072358094 1102 26-27 0.17765458483560792 1102 28-29 0.10900300856980749 1102 30-31 0.38104301078271874 1102 32-33 0.5623163337857093 1102 34-35 0.5050690543359977 1102 36-37 0.4378718326522275 1102 38-39 0.27154635108909275 1102 40-41 0.5712047544983037 1102 42-43 0.662407954512183 1102 44-45 0.3207982823845299 1102 46-47 0.6162771252319956 1102 48-49 0.3510569255224567 1102 50-51 0.5465094100891008 1102 52-53 0.6613392264746949 1102 54-55 1.0283399380068232 1102 56-57 1.32374799662351 1102 58-59 1.416567387185264 1102 60-61 1.4973930799419044 1102 62-63 1.2263578802891288 1102 64-65 1.4669127936813027 1102 66-67 1.8050773405621143 1102 68-69 1.8951113818943774 1102 70-71 2.0757425982455224 1102 72-73 2.548364082017276 1102 74-75 2.4350045761026315 1102 76-77 1.9882338102483992 1102 78-79 2.133441864926681 1102 80-81 2.1796416360389905 1102 82-83 1.6089379872545173 1102 84-85 1.9654964104501005 1102 86-87 1.8400871749920213 1102 88-89 2.0949381514427863 1102 90-91 2.2605952609031696 1102 92-93 1.5263518950362425 1102 94-95 1.7323333048765512 1102 96-97 2.12081734722495 1102 98-99 2.00095126782573 1102 100-101 2.215805397356789 1103 1 0.11659264347330023 1103 2 0.42291986335110465 1103 3 0.34101175437533726 1103 4 -0.1649750466523372 1103 5 -0.15583541754293861 1103 6 0.10776070105343649 1103 7 -0.03293673463910807 1103 8 0.014013338567956168 1103 9 0.24399108177602358 1103 10-11 0.29903981801312085 1103 12-13 0.21809551009426542 1103 14-15 -0.07064687630030875 1103 16-17 0.4525459256684812 1103 18-19 0.5128245077807492 1103 20-21 0.5233244228293614 1103 22-23 0.518525853482096 1103 24-25 0.4081596247996231 1103 26-27 0.026699498091971918 1103 28-29 0.19302646259870215 1103 30-31 0.032156774912564856 1103 32-33 0.2830592461957977 1103 34-35 0.5652153397739497 1103 36-37 0.4665471031593924 1103 38-39 0.2428333002518812 1103 40-41 0.2594490270002652 1103 42-43 0.5746366917424339 1103 44-45 0.9178710624123383 1103 46-47 0.7178684327336597 1103 48-49 0.8808504937990662 1103 50-51 0.7258828610955632 1103 52-53 0.6239895921882876 1103 54-55 0.6743458015140433 1103 56-57 0.6491197550711831 1103 58-59 0.9555717999278137 1103 60-61 0.9515353153284707 1103 62-63 0.6486664095764425 1103 64-65 0.628982853525347 1103 66-67 0.7299171519627095 1103 68-69 0.5623649030211375 1103 70-71 0.6319068368963094 1103 72-73 0.5341889021829047 1103 74-75 0.5908937739506648 1103 76-77 0.4802168242120075 1103 78-79 0.9456887367153506 1103 80-81 0.5910240938761397 1103 82-83 0.5390443766639343 1103 84-85 -0.06730447209841373 1103 86-87 -0.3116235183525937 1103 88-89 0.14886579455467697 1103 90-91 0.5731292632002152 1103 92-93 0.3112004714734091 1103 94-95 0.3581266918075805 1103 96-97 0.6736569168314297 1103 98-99 0.42565507003814673 1103 100-101 0.5989432804493333 1104 1 -0.11435940784425114 1104 2 -0.17613869957288486 1104 3 -0.013988476369043212 1104 4 0.034111205597419314 1104 5 -0.013766101930777097 1104 6 0.1733613286781548 1104 7 0.127984396931339 1104 8 0.1976166428274979 1104 9 0.3840298468320924 1104 10-11 0.21053781053700504 1104 12-13 0.29968672334822344 1104 14-15 0.08286736062801481 1104 16-17 0.5384520588541406 1104 18-19 0.4919652157045107 1104 20-21 0.232771097802825 1104 22-23 0.02350993211978647 1104 24-25 -0.15809862431200372 1104 26-27 0.2506138457776075 1104 28-29 0.665833237253743 1104 30-31 0.4011285779491516 1104 32-33 0.3273852418578045 1104 34-35 0.36168725818273373 1104 36-37 0.4113876587918597 1104 38-39 0.6494049002334279 1104 40-41 0.42560805602791163 1104 42-43 0.5973996249650142 1104 44-45 0.07528487938589734 1104 46-47 0.5205819866586197 1104 48-49 -0.06589370297974284 1104 50-51 0.17135796377682055 1104 52-53 -0.02586734629525722 1104 54-55 0.10370340915629583 1104 56-57 0.4447725309260946 1104 58-59 0.4001354623026323 1104 60-61 0.6665775415502644 1104 62-63 0.523268375518569 1104 64-65 0.3760639371009624 1104 66-67 0.89861436918547 1104 68-69 0.7129025374297377 1104 70-71 0.6225963011078584 1104 72-73 0.8501587208178201 1104 74-75 0.5230895374838234 1104 76-77 0.6504138799137031 1104 78-79 0.8375157707270766 1104 80-81 1.1031612483364306 1104 82-83 0.7617473163473569 1104 84-85 0.5082989244588809 1104 86-87 0.4539748018283092 1104 88-89 0.5705742259543705 1104 90-91 1.1135787532551333 1104 92-93 1.5132517890238262 1104 94-95 0.9811595959563668 1104 96-97 1.147755860022162 1104 98-99 0.6126618539229618 1104 100-101 0.7870230257075406 1105 1 0.3916494473107157 1105 2 0.2322104465371737 1105 3 0.3572955211009088 1105 4 0.15403530426914358 1105 5 0.23487399927099517 1105 6 0.32111591438340525 1105 7 0.23285473216220254 1105 8 0.2855356814106713 1105 9 0.437730036585414 1105 10-11 0.2730615297020904 1105 12-13 0.23994605288648785 1105 14-15 0.28052706967292806 1105 16-17 0.38560575904389083 1105 18-19 0.19768944087844886 1105 20-21 0.26297350134488795 1105 22-23 0.42445869872319264 1105 24-25 0.1976572264153873 1105 26-27 0.4653513536839995 1105 28-29 0.5702291891829034 1105 30-31 0.5426213040718579 1105 32-33 -0.010881677813287638 1105 34-35 -0.23834436737071485 1105 36-37 0.021571087001852618 1105 38-39 0.5546863676591016 1105 40-41 0.742559352675606 1105 42-43 0.286931566773994 1105 44-45 -0.023671477350347914 1105 46-47 -0.14981649531481622 1105 48-49 0.12278434888616374 1105 50-51 0.4652542319615165 1105 52-53 0.6133483399792468 1105 54-55 0.1348229537483263 1105 56-57 -0.2000092527551196 1105 58-59 -0.013590027893446432 1105 60-61 -0.49015870133398565 1105 62-63 0.3116934229568997 1105 64-65 -0.05783865619441997 1105 66-67 -0.6672300330915704 1105 68-69 -0.378811567567098 1105 70-71 -0.6475175508845545 1105 72-73 -0.8123649983472667 1105 74-75 -0.1683715630854401 1105 76-77 -0.3055317241343687 1105 78-79 -0.1685879610882317 1105 80-81 -0.24392920074642888 1105 82-83 -0.24723434083164264 1105 84-85 -0.22288387124004316 1105 86-87 -0.008960049531591352 1105 88-89 -0.369084218068398 1105 90-91 -0.7711777299833251 1105 92-93 -1.3750151306472702 1105 94-95 -0.8668796197299109 1105 96-97 -0.6029399021536754 1105 98-99 0.1851476224239086 1105 100-101 -0.04049120579151122 1106 1 0.002717558765823469 1106 2 -0.05432206120276817 1106 3 -0.17598621264521697 1106 4 0.24675976247348075 1106 5 -0.03385665088380563 1106 6 -0.0017943023348649945 1106 7 0.27031603247179703 1106 8 0.1976873841970459 1106 9 0.1654390149135878 1106 10-11 0.0873417154606031 1106 12-13 0.2485760838462454 1106 14-15 -0.09950389007939009 1106 16-17 0.13777294170643728 1106 18-19 0.17292163902086344 1106 20-21 0.32469950753684174 1106 22-23 0.29632402380059375 1106 24-25 0.1820612511831854 1106 26-27 0.20258045585117657 1106 28-29 0.3411657216906363 1106 30-31 0.466847310263816 1106 32-33 0.7438396844158106 1106 34-35 0.297878542846 1106 36-37 0.8558203130080457 1106 38-39 0.7594077298882027 1106 40-41 1.258658423883034 1106 42-43 1.3155306379814249 1106 44-45 0.7467619591821588 1106 46-47 0.4659342786789935 1106 48-49 0.6958493643660475 1106 50-51 0.19838116694602803 1106 52-53 0.3041765133538661 1106 54-55 -0.624968067923497 1106 56-57 -0.4346841753557378 1106 58-59 0.4308372166885981 1106 60-61 0.44703170114279445 1106 62-63 0.34033119385473043 1106 64-65 -0.10911574597770013 1106 66-67 0.6640006170632304 1106 68-69 1.549207008284295 1106 70-71 0.9119638732640531 1106 72-73 0.687170605367907 1106 74-75 0.8501268889269475 1106 76-77 0.5984543439770853 1106 78-79 0.8711179212647089 1106 80-81 0.9340893751049641 1106 82-83 0.5446774238742371 1106 84-85 0.5665898129704843 1106 86-87 0.6358154922640757 1106 88-89 0.988655720012094 1106 90-91 0.47464270345320614 1106 92-93 0.7454957052660447 1106 94-95 1.0228262626230311 1106 96-97 0.6354114910351782 1106 98-99 1.2142110899161658 1106 100-101 0.9708091038060083 1107 1 0.22457428391202328 1107 2 0.13792940078553784 1107 3 0.006396828290455403 1107 4 0.16963987943254466 1107 5 0.059710600578185336 1107 6 0.18680218889321054 1107 7 -0.25709624822995636 1107 8 -4.120310076274336E-4 1107 9 -0.08236800263027533 1107 10-11 0.2615010721857516 1107 12-13 0.3372091247819142 1107 14-15 -0.020739270196351356 1107 16-17 -0.007694894550873244 1107 18-19 0.36517310100916944 1107 20-21 0.3563150046128598 1107 22-23 0.32940150918071254 1107 24-25 0.38838435059838616 1107 26-27 0.5558333798277886 1107 28-29 0.5184318035619881 1107 30-31 0.11999058511761973 1107 32-33 0.4214712633631805 1107 34-35 0.9036899463547812 1107 36-37 1.32054984517179 1107 38-39 1.298150419946687 1107 40-41 0.9607783069239701 1107 42-43 1.4134838543556967 1107 44-45 1.5954461697084739 1107 46-47 1.1887047465152492 1107 48-49 1.7870408848338784 1107 50-51 1.034821225425567 1107 52-53 0.7796882092602999 1107 54-55 1.1237526923104184 1107 56-57 1.198356760316777 1107 58-59 1.6848576845248537 1107 60-61 1.897872344417653 1107 62-63 1.5976411353752056 1107 64-65 1.4793345464199597 1107 66-67 1.4211278100456823 1107 68-69 1.5311149030211375 1107 70-71 1.4751948674160964 1107 72-73 1.302667681391295 1107 74-75 1.418474842143322 1107 76-77 1.9670581451466767 1107 78-79 1.8745901434869303 1107 80-81 2.117386158730696 1107 82-83 2.1523163127631264 1107 84-85 1.888227239871071 1107 86-87 1.511546486416119 1107 88-89 1.0023618603629707 1107 90-91 1.1148108321081764 1107 92-93 1.2010878105292022 1107 94-95 1.6894929292896954 1107 96-97 2.380842777584885 1107 98-99 1.6202783413781567 1107 100-101 1.3397784020516212 1108 1 0.10149508769452709 1108 2 0.027704814497120367 1108 3 0.02250202923607958 1108 4 0.20318006855784176 1108 5 0.06916686535360128 1108 6 0.1176532527229952 1108 7 0.26639665957146264 1108 8 0.3370584181649434 1108 9 -0.10453112319764557 1108 10-11 0.30885627313137576 1108 12-13 0.06896148884811026 1108 14-15 0.2509067108911154 1108 16-17 0.523924490791913 1108 18-19 0.08118964947252039 1108 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0.01862191642943145 2316 42-43 0.24276291665498917 2316 44-45 0.7464577304021205 2316 46-47 0.5427710599385023 2316 48-49 0.45505228496233485 2316 50-51 1.1262028888809326 2316 52-53 0.7771191662288288 2316 54-55 0.7268154732995029 2316 56-57 0.6879200230014249 2316 58-59 1.0875150384105332 2316 60-61 0.9959174632358874 2316 62-63 1.2029718996655028 2316 64-65 1.1553541353730736 2316 66-67 0.6990059410668792 2316 68-69 0.3369313770095772 2316 70-71 0.0985892155214323 2316 72-73 0.9275873987965646 2316 74-75 0.49125101426919926 2316 76-77 0.4513026859302336 2316 78-79 0.4195283258889866 2316 80-81 0.3085189461423852 2316 82-83 0.1368017013308851 2316 84-85 -0.24456017181807965 2316 86-87 -0.27871523484271066 2316 88-89 -0.5785027831823051 2316 90-91 -0.591374941816035 2316 92-93 -0.4071130565228209 2316 94-95 0.08424244759412858 2316 96-97 -0.017900799807534895 2316 98-99 -0.39810637281580696 2316 100-101 -0.12692439178267279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 15.0 6 33.0 7 72.0 8 121.0 9 246.0 10 406.0 11 665.0 12 848.0 13 1042.0 14 1355.0 15 2004.0 16 2675.0 17 3447.0 18 3137.0 19 2007.0 20 1882.0 21 1954.0 22 1908.0 23 2141.0 24 2243.0 25 2303.0 26 2557.0 27 2944.0 28 3571.0 29 4104.0 30 5026.0 31 6046.0 32 7401.0 33 9458.0 34 13583.0 35 20323.0 36 24286.0 37 23281.0 38 9749.0 39 435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.35784611048824 19.521385115885824 15.486977778594666 12.633790995031276 2 13.732191638185537 29.62037411341002 35.50708659488197 21.140347653522472 3 27.029576231220105 32.520379977093576 29.58541828716322 10.864625504523099 4 10.543142731148022 18.707432995247196 40.329633985006616 30.41979028859817 5 10.38138577719525 45.70075885173911 30.054571178333223 13.863284192732417 6 23.030139704666418 41.57454079976481 16.25804633956625 19.137273156002525 7 22.777189124962486 42.15516343792299 19.219956759537705 15.84769067757682 8 24.190160038708175 28.628738370704284 19.342450986997235 27.838650603590303 9 25.120503696263313 25.773397928622614 22.157980805154555 26.948117569959518 10-11 23.73081210873853 28.66300366300366 23.846276354637556 23.759907873620246 12-13 34.32165759188599 28.762869549772468 21.99812583831987 14.917347020021682 14-15 26.24071340638072 29.196805350547855 31.57809313236114 12.984388110710283 16-17 15.846159499733576 32.54824741384063 31.32942985061829 20.276163235807513 18-19 15.744231269809674 36.94345342908328 32.84815262827482 14.464162672832229 20-21 20.74031200044099 28.879946591208373 30.769466340824035 19.610275067526597 22-23 17.51816770328994 31.173727035808675 30.393426859108047 20.914678401793342 24-25 21.86602226020055 31.178982058302857 31.256776375029858 15.69821930646673 26-27 21.955402562162924 27.176506258402984 36.49872443120044 14.36936674823365 28-29 16.211241942151926 33.51421702981212 35.17555007732472 15.098990950711233 30-31 17.456506493784893 34.84809045056853 31.86930970806295 15.826093347583626 32-33 16.88930537946579 34.611591912732976 31.029772951386974 17.46932975641426 34-35 19.126861146668464 31.096690818445182 31.817569347044522 17.958878687841835 36-37 20.21872817924563 31.28897110166481 34.468829243792186 14.023471475297375 38-39 20.766933643700515 30.791283174321197 34.189803825479714 14.251979356498568 40-41 15.37950359062304 33.96928447840267 34.94617893399072 15.70503299698357 42-43 14.842931776840015 37.22415831153957 29.32419934710577 18.608710564514645 44-45 17.999301782903483 33.57995504461852 29.136476943524038 19.28426622895396 46-47 19.265397618697634 34.12220098975942 27.558920084276544 19.053481307266402 48-49 20.388070031451065 33.43306618811229 28.88353305424467 17.29533072619197 50-51 19.01522970113281 34.38764229036575 32.3688570527328 14.228270955768634 52-53 17.500444041574543 36.9678391405805 29.419744844524203 16.111971973320756 54-55 13.646984605302242 34.32034568400293 30.99151408245825 21.041155628236577 56-57 19.059970662362996 35.004578263812114 27.17773769457621 18.757713379248674 58-59 20.763077789959148 32.83733379064512 27.04121318282876 19.358375236566978 60-61 17.43941055055705 32.845392016953404 31.46792755602648 18.247269876463058 62-63 20.032033123352026 32.05394794464333 29.867365719658107 18.046653212346534 64-65 18.005732729845107 36.00901557514102 29.977706050602364 16.007545644411508 66-67 16.716533094056146 32.031702185650104 29.833802394200998 21.41796232609275 68-69 15.682629706075101 36.43131442430775 28.396305574099824 19.489750295517325 70-71 19.635266736081338 34.47755252036504 25.66178722361732 20.2253935199363 72-73 17.77513734665254 31.636584125973066 29.48252313609721 21.105755391277185 74-75 17.206764131240316 36.468368928114266 27.486785935212804 18.83808100543262 76-77 18.8561489039829 31.501534240198932 28.458471394535533 21.183845461282637 78-79 16.7153593149955 32.167772204153884 29.637285706410893 21.479582774439727 80-81 18.031843057031473 34.78941259696391 27.118659639430515 20.0600847065741 82-83 17.01908057946158 31.898257327493795 28.59361122170837 22.489050871336254 84-85 16.530383491666818 31.674966158896993 28.21092347929415 23.58372687014204 86-87 17.10735854372619 33.1018093173104 27.940906251148434 21.849925887814976 88-89 17.723383535550887 31.54379474867247 26.587065834522548 24.145755881254097 90-91 15.790924291515335 30.10546882177484 29.14479607523688 24.95881081147295 92-93 16.661664819045402 33.898133800444654 27.123896786363943 22.316304594146 94-95 17.08886343731052 33.529732411360115 25.800040423095062 23.581363728234304 96-97 13.73640676055993 32.93552723853973 27.9970968423567 25.330969158543642 98-99 17.27690328903044 34.162124088932444 24.544925583389478 24.01604703864764 100-101 16.622772901827002 32.530485750858986 24.987597459469725 25.859143887844287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2807.0 1 1836.0 2 851.5 3 796.5 4 701.5 5 794.0 6 1206.5 7 1964.0 8 2556.5 9 2597.5 10 2178.0 11 1723.0 12 1625.5 13 1582.0 14 1399.5 15 1158.0 16 1003.5 17 920.5 18 837.5 19 780.0 20 793.0 21 829.5 22 857.0 23 886.5 24 950.0 25 1062.0 26 1169.0 27 1308.0 28 1453.0 29 1568.0 30 1677.0 31 1731.0 32 1838.0 33 2019.0 34 2247.5 35 2408.5 36 2548.0 37 2668.0 38 2609.5 39 2443.0 40 2260.0 41 2092.0 42 1951.0 43 1901.0 44 1969.5 45 2224.5 46 3656.0 47 9027.0 48 26175.0 49 25414.0 50 8866.5 51 4232.5 52 1799.0 53 1149.0 54 951.5 55 887.0 56 964.5 57 984.5 58 934.5 59 952.0 60 963.0 61 958.0 62 809.5 63 430.5 64 210.5 65 150.0 66 102.5 67 76.5 68 53.0 69 34.0 70 28.5 71 19.5 72 16.0 73 18.5 74 13.5 75 7.0 76 4.5 77 2.5 78 1.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03184849913947805 2 0.004287297961083584 3 0.0 4 6.124711372976549E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011636951608655442 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.0623556864882743E-4 20-21 0.0024498845491906195 22-23 0.001531177843244137 24-25 0.013474365020548407 26-27 0.007655889216220686 28-29 3.0623556864882743E-4 30-31 9.187067059464823E-4 32-33 0.0024498845491906195 34-35 0.0 36-37 0.0067371825102742035 38-39 0.015618014001090197 40-41 3.0623556864882743E-4 42-43 0.0 44-45 0.0 46-47 6.124711372976549E-4 48-49 0.002143648980541792 50-51 0.003981062392434756 52-53 0.0 54-55 0.001531177843244137 56-57 9.187067059464823E-4 58-59 0.0 60-61 0.0012249422745953097 62-63 0.002756120117839447 64-65 0.0 66-67 0.002143648980541792 68-69 0.0 70-71 0.0018374134118929646 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0024498845491906195 80-81 0.002143648980541792 82-83 0.011024480471357788 84-85 0.006124711372976548 86-87 0.004287297961083584 88-89 0.0 90-91 0.0012249422745953097 92-93 0.0 94-95 0.0 96-97 0.001531177843244137 98-99 0.0018374134118929646 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 163273.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.007294531245215 #Duplication Level Percentage of deduplicated Percentage of total 1 82.85459808505901 27.348061222614884 2 9.452237808951235 6.2398559467885075 3 2.931789504935798 2.903113190790884 4 1.3452831589104133 1.7761662981631992 5 0.7199584353892972 1.1881940063574503 6 0.445335114673792 0.881958437708623 7 0.3469902768499963 0.8017247187226302 8 0.250501002004008 0.6614688282814672 9 0.23194537222593334 0.6890300294598617 >10 1.1356045424181698 7.059954799630068 >50 0.11318934164625546 2.7316212723475406 >100 0.10205596377941067 7.603829169550385 >500 0.0222667557336896 4.949379260502349 >1k 0.0445335114673792 27.891935592535198 >5k 0.0037111259556149336 7.273707226546949 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6360 3.8953164332130847 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5516 3.3783907933338644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4570 2.7989930974502824 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3464 2.1216000195990765 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3335 2.042591242887679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2768 1.6953201080399087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 1.623660984976083 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2537 1.5538392753241503 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2441 1.4950420461435754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2119 1.2978263399337306 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2114 1.2947639842472425 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1865 1.1422586710601261 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1693 1.0369136354449298 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1536 0.9407556668891979 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1429 0.8752212551983487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1338 0.8194863817042621 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1321 0.8090743723702021 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1302 0.7974374207615466 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1247 0.7637515082101756 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1217 0.7453773740912459 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1211 0.74170254726746 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1180 0.7227159420112327 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1090 0.6675935396544438 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1073 0.6571815303203836 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1030 0.6308452714165845 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1009 0.6179833775333338 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 932 0.5708230999614143 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 877 0.5371371874100433 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 766 0.46915289117000364 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 728 0.4458789879526927 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.4085182485775358 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 626 0.3834069319483319 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 620 0.37973210512454597 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 617 0.37789469171265305 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 595 0.36442032669210467 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 574 0.3515584328088539 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 555 0.33992148120019844 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 524 0.32093487594397113 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 490 0.30011085727585085 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 470 0.28786143452989776 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.2805117808823259 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 458 0.2805117808823259 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 442 0.27071224268556343 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 427 0.2615251756260986 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.24437598378176428 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 379 0.2321265610358112 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 325 0.1990531196217378 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 322 0.19721570620984488 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 320 0.19599076393524953 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 320 0.19599076393524953 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 311 0.19047852369957066 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.18986605256227299 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 294 0.18006651436551052 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 270 0.1653672070703668 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 258 0.15801755342279494 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 253 0.15495519773630667 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 251 0.15373025546171137 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 244 0.14944295750062778 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 242 0.14821801522603248 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 236 0.14454318840224656 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 222 0.13596859248007936 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 219 0.13413117906818642 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 208 0.1273939965579122 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 202 0.12371916973412628 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 201 0.12310669859682863 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 191 0.11698198722385207 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 181 0.11085727585087553 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 173 0.1059575067524943 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 170 0.10412009334060132 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 165 0.10105773765411305 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0012249422745953097 0.0 18-19 0.0 0.0 0.0 0.0012249422745953097 0.0 20-21 0.0 0.0 0.0 0.0024498845491906195 0.0 22-23 0.0 0.0 0.0 0.006430946941625376 0.0 24-25 0.0 0.0 0.0 0.017149191844334333 0.0 26-27 0.0 0.0 0.0 0.024192609923257367 0.0 28-29 0.0 0.0 0.0 0.0413418017675917 0.0 30-31 0.0 0.0 0.0 0.07012794522058148 0.0 32-33 0.0 0.0 0.0 0.09064572832005291 0.0 34-35 0.0 0.0 0.0 0.11085727585087553 0.0 36-37 0.0 0.0 0.0 0.1350498857741329 0.0 38-39 0.0 0.0 0.0 0.1687357983255039 0.0 40-41 0.0 0.0 0.0 0.2391699791147342 0.0 42-43 0.0 0.0 0.0 0.36105173543696756 0.0 44-45 0.0 0.0 0.0 0.4633344153656759 0.0 46-47 0.0 0.0 0.0 0.718734879618798 0.0 48-49 0.0 0.0 0.0 0.983322410931385 0.0 50-51 0.0 0.0 0.0 1.1223533590979526 0.0 52-53 0.0 0.0 0.0 1.2619967784018178 0.0 54-55 0.0 0.0 0.0 1.423076687511101 0.0 56-57 0.0 0.0 0.0 1.8021963214983494 0.0 58-59 0.0 0.0 0.0 2.3080974809062123 0.0 60-61 0.0 0.0 0.0 2.87034598494546 0.0 62-63 0.0 0.0 0.0 3.3584854813716905 0.0 64-65 0.0 0.0 0.0 3.8016083492065436 0.0 66-67 0.0 0.0 0.0 4.434291034035021 0.0 68-69 0.0 0.0 0.0 5.058705358509981 0.0 70-71 0.0 0.0 0.0 5.4880476257556365 0.0 72-73 0.0 0.0 0.0 5.979861949005652 0.0 74-75 0.0 0.0 0.0 6.524961261200566 0.0 76-77 0.0 0.0 0.0 6.9959515657824625 0.0 78-79 0.0 0.0 0.0 7.474291524011931 0.0 80-81 0.0 0.0 0.0 8.142191299235023 0.0 82-83 0.0 0.0 0.0 8.901961745052764 0.0 84-85 0.0 0.0 0.0 10.303295707189799 0.0 86-87 0.0 0.0 0.0 11.592241215632715 0.0 88-89 0.0 0.0 0.0 12.668353003864691 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGT 50 0.0016117826 37.999878 4 GGACAAT 50 0.0016117826 37.999878 6 GAGGGTT 75 6.8860427E-6 37.999874 9 ATGGGGT 55 0.0025732156 34.54534 5 GAGTGGG 55 0.0025732156 34.54534 7 GTGGGTA 55 0.0025732156 34.54534 9 TCGGGAG 55 0.0025732156 34.54534 3 GGGATGT 55 0.0025732156 34.54534 7 GTCGGGA 55 0.0025732156 34.54534 2 AGTGGGT 60 0.003941178 31.666567 8 TATAGGA 60 0.003941178 31.666567 5 GGGAGTG 75 3.0670344E-4 31.666563 5 GGAGGGT 110 2.7146198E-6 30.227173 8 GGGAGGG 130 3.0100637E-7 29.230675 7 TGCCATT 35 0.008383059 27.14277 12-13 TGGGAGT 70 0.008370997 27.14277 6 ATGGGGA 175 4.8949005E-9 27.142769 5 ATGGGAG 380 0.0 26.249916 5 TGGGAGA 130 9.952793E-6 25.57684 6 AGTACTT 1880 0.0 25.392204 12-13 >>END_MODULE